Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2_120917_005.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH5_120917_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH5_120917_005.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_007.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH3_130214_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH4_130214_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH6_120917_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH1_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_130214_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH4_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH2_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH3_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH1_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH3_130214_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH5_120917_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2plus_130214_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2_120917_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2plus_130214_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH6_120917_004.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_004.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_130214_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH3_130214_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH4_130214_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH6_120917_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_006.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_130214_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH5_120917_006.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH4_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH2_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH3_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH1_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH4_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH3_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH2_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH6_120917_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH4_120917_001.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2plus_130214_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH3_120917_002.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH4_130214_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2_120917_003.raw.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH4_130214_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH3_130214_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH6_120917_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH2_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_130214_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH3_130214_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_130214_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH4_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH6_120917_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH4_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH5_120917_006.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH2_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH6_120917_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH4_120917_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH3_130214_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH4_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_004.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH2_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH5_120917_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH6_120917_004.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2plus_130214_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_007.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH3_120917_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH3_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2plus_130214_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2_120917_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH5_120917_005.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_120917_006.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH5_120917_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH1_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH1_130214_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH4_130214_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2_120917_003.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH3_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2plus_130214_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH3_001.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH1_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH2_120917_005.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH4_130214_002.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD001356/MH_20131105_MH1_001.zipprimaryOK200003790sacha.baginsky@biochemtech.uni-halle.deSacha BaginskyMass SpectrometryShotgun proteomicsNot availableZinc cupriavidushttp://www.ebi.ac.uk/pride/archive/projects/PXD001356Prokaryotic CellStrains used for the experiments were Cupriavidus metallidurans CH34(pMOL28, pMOL30) wild type stain, its plasmid-free derivative strain AE104 34, the AE104 ΔzupT deletion mutant 10, and the Δe4 (ΔzntA ΔcadA ΔdmeF ΔfieF) quadruple deletion mutant. The bacteria were cultivated in Tris-buffered mineral salts medium 34 containing 2 g sodium gluconate L-1 (TMM) aerobically with shaking at 30°C. TMM contained 34 nM zinc chloride as part of an added trace element solution. Solid Trisbuffered media contained 20 g agar L-1.PrideAPEX - Absolute Protein Expressioniodoacetamide derivatized residuemonohydroxylated residuewas carried out by ProteinLynx Global Server (PLGS 3.0, Apex3D algorithm v. 2.128.5.0, 64 bit, Waters, Eschborn, Germany) with automated determination of chromatographic peak width as well as MS TOF resolution with following values: Lock mass: 785.8426 Da/e, Lock mass window: 0.25 Da, Low/high energy threshold: 200/20 counts, elution start time: 5 min, intensity threshold: 750 counts. Databank search query (PLGS workflow) was carried out as follows: Peptide and fragment tolerances was set to automatic, two fragment ions match per peptide, at least five fragment ions for protein identification, and min 2 peptides per protein. A maximum protein mass was set to 250 kDa. Primary digest reagent was trypsin with one missed cleavage allowed. According to digest protocol fixed (carbamidomethyl on Cys) as well as variable (oxidation on Met) modifications were set. The false discovery rate (FDR) was set to 4% at the protein level. Rabbit glycogen phosphorylase B (P00489, 10 fmol/injection) was defined as internal calibration standard. The modified NCBI data base (http://www.ncbi.nlm.nih.gov) to identify proteins contained 5804 proteins encoded by the open reading frames Rmet_0001 to Rmet_5941 of the two chromosomes of C. metallidurans, glycogen phosphorylase B (P00489, added as internal standard at a concentration of 10 fmol, Waters, Eschborn, Germany) as well as common contaminants (ftp://ftp.thegpm.org/fasta/cRAP/crap.fasta).ProteomicsBaginsky, SachaSynapt MSPARTIALMartin-Luther-University Halle-Wittenberg, Plant BiochemistryCupriavidus Metallidurans25315396 Herzberg M, Dobritzsch D, Helm S, Baginsky S, Nies DH. The zinc repository of Cupriavidus metallidurans. Metallomics. 2014 Nov;6(11):2157-65 10.1039/c4mt00171kpflanzen@biochemtech.uni-halle.deBiologicalInstitut fuer Biochemie und BiotechnologieGermanyZinc is a central player in the metalloproteomes of prokaryotes and eukaryotes. We used a bottom-up quantitative proteomic approach to reveal the repository of the zinc pools in the proteobacterium Cupriavidus metallidurans. About 60% of the theoretical proteome of C. metallidurans was identified, quantified, and the defect in zinc allocation was compared between a ΔzupT mutant and its parent strain. In both strains, the number of zinc-binding proteins and their binding sites exceeded that of the zinc ions per cell, indicating that the totality of the zinc proteome provides empty binding sites for the incoming zinc ions. This zinc repository plays a central role in zinc homeostasis in C. metallidurans and probably also in other organisms.The zinc repository of Cupriavidus metallidurans.Herzberg Martin M, Dobritzsch Dirk D, Helm Stefan S, Baginsky Sacha S, Nies Dietrich H DHgluconate, (65)Zn-labeled, bacteria, AtRLP17, F18B13.16, Ralstonia metallidurans CH34, sales, Sprain, A4, eubacteria, F18B13_16, TYPE, LGMD2C, element, strain, hexite, DAGA4, Ion Level, Sodium-23, sodio, ionic compounds, Strain, sodium, prokaryotes, atomo, atomus, cultivar, Sodium, Sodium Ion, MAM, atome, Sprains, SCG3, Level, natrium, Sodium Ion Level, Wautersia metallidurans CH34, Eubacteria, Element, DMDA1, ionic compound, Receptor Like Protein 17, atoms, Salt, TOO MANY MOUTHS, Na, Natrium, Salze, Salz, sel, free, ecotype, Monera, zinc chloride, Solution, Cupriavidus metallidurans str. CH34, sal, salts, Mineral, salt, 11Na, DMDA, Bacteria <bacteria>, DEL, Prokaryotae, prokaryote, anatomical unit, sels, SCARMD2, body organ, solid., Cupriavidus metallidurans strain CH34, Strains, fungi, Procaryotae, TMM, elements, Sodium 23, Strains and Sprains, atom, ProkaryotaZn(II), Wautersia metallidurans, 30Zn, Zink, Ralstonia metallidurans, Zn2+, Ralstonia metallidurans Goris et al. 2001, Zn, cinc, zincum, Wautersia metallidurans (Goris et al. 2001) Vaneechoutte et al. 2004, zinc, Cupriavidus metallidurans (Goris et al. 2001) Vandamme and Coenye 2004.IPP2A2, rabbits, ion, Hgfr, Brain Ig-like molecule with tyrosine-based activation motifs, FEZL, cleavage, 5730555F13Rik, protein, rabbit, Reading Frame, Long Term, 5730420M11Rik, peptide, Polypeptides, protein polypeptide chains, Methionine, ClvPrd, peptido, HGFR, Plgs, Min, ORFs, Work Flow, protein aggregate, Gpi-1, Unassigned, Effect, Injectables, Unassigned Reading Frame, WMS, DmelCG42273, ASD2, SET, Unidentified Reading Frames, me75, F, peptides, kDa, TAF-I, MUB3_18, TACHD, Nlk, M, phosphatase 2A inhibitor I2PP2A, Open, min, mAPC, proteins, Inhibitory receptor SHPS-1, MUB3.18, D17Mit170, T1, AI047805, DmelCG4299, set, IGAAD, Protein Coding, Sirp-alpha-2, Sirp-alpha-3, DmelCG10574, Sirp-alpha-1, SHP substrate 1, Lepus cuniculus, RG7MT1, GPHYSD2, Racemethionine, Protein Coding Regions, Long-Term Effects, reagents, SGS, ratio, NK/GPI, phapii, Gpi, proto-oncogene c-Met, European rabbit, PRO, 2-Amino-4-(methylthio)butyric acid, Longterm Effect, Dmel_CG7826, StF-IT-1, Tl3, 9130215G10Rik, Tl2, CYS, ACMICD, Workflows, mOC-X, Dm1, Ionen, SHPS1, Algorithm, Methionin, methionine, NK|GPI, Hmet, reactif, Region, fixed, and GLY protein 2, Dmel_CG7835, Mnb, MNB, HGF receptor, HGF/SF receptor, ORF, and GLY protein 1, ORG, Org, HLA-DR-associated protein II, DI-2, I-2Dm, proportionality, Phosphorylase ab, fmol, common, Gpi1-r, reagent, rate, Gpi1-s, MASS, CG4299, inhibitor of granzyme A-activated DNase, hCMT1c, AW124434, Signal-regulatory protein alpha-1, study protocol, c-Met, Gpi1-t, Signal-regulatory protein alpha-2, beta Trypsin, CD172a, Signal-regulatory protein alpha-3, Crap, I-2PP1, MyD-1 antigen, 1110030K05Rik, TAF-IBETA, Chromosome, Bit, BIT, Phosphorylase, MARP, TAF-Ibeta, Polypeptide, mSIRP-alpha1, i2pp2a, time, CD172A, Work Flows, Bglap-rs1, Gpi1s, SHPS-1, CARP, breadth, 2-amino-4-(methylsulfanyl)butanoic acid, Effects, False, Open Reading Frame, AI461847, FBN, Gene, Crap., protein-containing complex, CD172 antigen-like family member A, CG7826, PHAPII, period, Unidentified Reading, Carp, method, HGF, template-activating factor I, polypeptide chain, TOF, ECTOL1, Injection, method used in an experiment, AI835480, CG7835, Gene Products, Unassigned Reading Frames, CG42273, SIRP, F15E12.6, Pgi, Low, RCCP2, ZNF312, Japanese white rabbit, Frame, F15E12_6, proportion, sORF, reactivo, Longterm, scatter factor receptor, Gpi-1r, P84, iones, Gpi-1s, ipp2a2, Phi, beta-Trypsin, Coding Region, Gpi-1t, 2pp2a, Long-Term, ions, CG10574, AUTS9, OCTD, Unidentified Reading Frame, Injectable, Glycogen, 2PP2A, 2-amino-4-(methylthio)butanoic acid, taf-ibeta, p84, dSET, dSet, peptidos, Long-Term Effect, AI838057, metionina, FEZ, Protein Coding Region, DYRK1, PTPNS1, cou, Macrophage fusion receptor, DL-Methionine, AI447866, protein complex, Proteins, igaad, MF, Amf, Peptide, polypeptide, Lr, native protein, natural protein, I-2PP2A, Long Term Effects, Dm I-2, Protein, I2PP2A, MFS1, Dyrk1, Small Open Reading Frames, WMS2, ATCMPG1, MET, Met, Data Base, NK, ATCMPG2, AU020952, VSD1, MARP1, SHP-1, tyrosine-protein kinase Met, CC1, Tripcellim, Alrp, MFR, MYD-1, Ptpns1, Unidentified, Longterm Effects, ME-IV, plan specification, Protein Gene Products, Trypure, domestic rabbit, Gene Proteins, ZFP312, dSET/TAF-Ibeta, 2610030F17Rik, Ion, concentration, Macrophage membrane protein MFP150, SSKS, Calibrations, Bra, alpha-amino-gamma-methylmercaptobutyric acid, quotient, Small Open Reading Frame, Peptid, Par4, variable, AA407739, 5730455C01Rik, SF receptord230, ion, number, Eukaryotes, Gene, dTAFII250, eubacteria, cellular zinc ion homeostasis, EfW1, presence, zinc, Cupriavidus metallidurans (Goris et al. 2001) Vandamme and Coenye 2004, Mendosicutes, dmTAF[[II]]230, dmTAF1, Roles, Taf230, Sites, Gene Products, Concepts, Sprains, TAF250, Site, zinc homeostasis., Taf200, dTAF[[II]]250, TFIID TAF250, Metabacteria, cel, cell, iones, ligand, Zn, Taf1p, proteins, number of, Eucarya, ions, Monera, ecotype, eucaryotes, Wautersia metallidurans, dTAF250, Combining Site, 30Zn, Zink, Prokaryotae, has or lacks parts of type, Role Concepts, Procaryotae, Strains, TAF, Combining Sites, Zn(II), bacteria, Archaebacteria, dTAF[[II]]230, TAF[[II]]250, Eukaryote, Ralstonia metallidurans Goris et al. 2001, Sprain, Proteins, TAF200, total expressed protein, l(3)84Ab, zincum, Eukaryotas, extra or missing physical or functional parts, BG:DS00004.13, Binding Site, TAFII-250, TAF250/230, Wautersia metallidurans (Goris et al. 2001) Vaneechoutte et al. 2004, Cell, eukaryotes, strain, Binding, dTAF230, Concept, mereological quality, count in organism, TAFII250, Ralstonia metallidurans, Zn2+, Role Concept, Ionen, p230, Protein, Strain, Role, TAF[[II]]250/230, TFIID, prokaryotes, cultivar, cinc, Taf[[II]]250, TAF[[II]]230, not Bacteria Haeckel 1894, archaea, Eukarya, Combining, TAF[II]250, CG17603, TAF[[II]], Protein Gene Products, Gene Proteins, Eukaryotae, DmelCG17603, Ion, Taf250, prokaryote, SR3-5, cardinality, Eucaryotae, quantitative, Strains and Sprains, Proteomes, Prokaryota, TAF230, presence or absence in organism, TAF1d230, ion, number, Eukaryotes, Gene, dTAFII250, eubacteria, cellular zinc ion homeostasis, EfW1, presence, zinc, Cupriavidus metallidurans (Goris et al. 2001) Vandamme and Coenye 2004, Mendosicutes, dmTAF[[II]]230, dmTAF1, Roles, Taf230, Sites, Gene Products, Concepts, Sprains, TAF250, Site, zinc homeostasis., Taf200, dTAF[[II]]250, TFIID TAF250, Metabacteria, cel, cell, iones, ligand, Zn, Taf1p, proteins, number of, Eucarya, ions, Monera, ecotype, eucaryotes, Wautersia metallidurans, dTAF250, Combining Site, 30Zn, Zink, Prokaryotae, has or lacks parts of type, Role Concepts, Procaryotae, Strains, TAF, Combining Sites, Zn(II), bacteria, Archaebacteria, dTAF[[II]]230, TAF[[II]]250, Eukaryote, Ralstonia metallidurans Goris et al. 2001, Sprain, Proteins, TAF200, total expressed protein, l(3)84Ab, zincum, Eukaryotas, extra or missing physical or functional parts, BG:DS00004.13, Binding Site, TAFII-250, TAF250/230, Wautersia metallidurans (Goris et al. 2001) Vaneechoutte et al. 2004, Cell, eukaryotes, strain, Binding, dTAF230, Concept, mereological quality, count in organism, TAFII250, Ralstonia metallidurans, Zn2+, Role Concept, Ionen, p230, Protein, Strain, Role, TAF[[II]]250/230, TFIID, prokaryotes, cultivar, cinc, Taf[[II]]250, TAF[[II]]230, not Bacteria Haeckel 1894, archaea, Eukarya, Combining, TAF[II]250, CG17603, TAF[[II]], Protein Gene Products, Gene Proteins, Eukaryotae, DmelCG17603, Ion, Taf250, prokaryote, SR3-5, cardinality, Eucaryotae, quantitative, Strains and Sprains, Proteomes, Prokaryota, TAF230, presence or absence in organism, TAF1Zn(II), Wautersia metallidurans, 30Zn, Zink, Ralstonia metallidurans, Zn2+, Ralstonia metallidurans Goris et al. 2001, Zn, cinc, zincum, Wautersia metallidurans (Goris et al. 2001) Vaneechoutte et al. 2004, zinc, Cupriavidus metallidurans (Goris et al. 2001) Vandamme and Coenye 2004.379trueThe zinc repository of Cupriavidus metalliduransZinc is a central player in the metalloproteomes of prokaryotes and eukaryotes. We used a top-down quantitative proteomic approach to reveal the repository of the zinc pools in the proteobacterium Cupriavidus metallidurans. About 60% of the theoretical proteome of C. metallidurans were identified, quantified, and compared between a ΔzupT mutant defect in zinc allocation and its parent strain. In both strains, the number of zinc-binding proteins and their binding sites exceeded that of the zinc ions per cell, indicating that the totality of the zinc proteome provides empty binding sites for incoming zinc ions. This zinc repository plays a central role in zinc homeostasis in C. metallidurans and probably also in other organisms.2016-05-042014-09-23PXD0013562806551646099180498295358511145278728421880905811363336351524260119832758385NCBITaxon:1280688885618961114301187263555571145262364934613126957274670738538836411354615821582368895476272112329442229118099232336545833346079251936591432059712938655819149315242592637371250232NCBITaxon:1272111957748186614885843451358672217554374081691804542668344530586126683514982250581496367458554528113208147956410039237514636942049433208964208963445974137979128384400667935293671521659940483216597216595455013152826930421659156271616968332183217292042779447623764543172030949801975255569781439843948840479NCBITaxon:2157525338551531051067439899037122857759515205968762311210884555588845587077918178081736309431794571698936160488409859NCBITaxon:371621047826794015059071483643204210613204913816936303555432046632633311994565453511025411103217459216129288676410026420369456941016763410210761277351131658239254727933428146135843017463345774449167547604893212873567568403019172651813019411192943936711041131023932025559863258278839659104759347495165299835525663175854310243260866310245980875741755518320196447145268045796190832331156114155421932555217634123598755529NCBITaxon:475185025476113615741465477180066556484337677299767982510216998238976413735115571108972444474153NCBITaxon:5055789969844100036465349334147147493548019467512991143597412764731370863265478765698353221599838192011103481985247946409860995861571871766859919157295137221171101107687834974198601488406334249235152335985988655974649987039202111072943311711397215987419085037117082841823502414511391902197822474636046147123743759392532566489050794526591972211915853101774208588596NCBITaxon:615750521028307989511051989972375059576131720002634196102641761792940772275689964975157244NCBITaxon:22023717NCBITaxon:170361220199985705061747679274506272491596153335541840052464321368615059686662941786661347515363291018194301086731018557303963558633149286287132161865368595105135260710185687294296110091416333NCBITaxon:619184023210007348824187878645159812863577553470951451225786260704NCBITaxon:1335626260705260707658457405416233150561007133705654195NCBITaxon:305550445791399878168119011928752829126806315327341478281055524NCBITaxon:9606469008150251930283672859300852NCBITaxon:56938268830640179469915101184991515482428381116041937047592723708281677285035158199011951953267386370249240536088385816224888175626158355837153337605726711935019980861193502167879371137127240719065037145712562745757270903113335503728142433747924910078711082NCBITaxon:961559463318586260799141262200298492670NCBITaxon:1263719281249212528303856838569591169266100658123756127504542666255151978837503600941293497905435247214012576499085151514389923745374744544641524323592887051580595536555088157932716029385114978633892894535936479437NCBITaxon:68544127542251171590109013416738715895521012966103061296715851584515849376593934327159100226376733889423451287689467041464792936576164280342164133159928038280353690910891768946218121410803481793922878892950271131174934681922732189038210760124719040072711431140206411981087135547743658142450722953379698204232124223129163218033470871453720916457331575469928777020206032811431933829355083329129221148725264999728303572672710379085507176299707678926837041521405329412816078395639242273775962073220865955516936053307972551811354915974953502611595561432138406072587814144715094933491949634016143347936046316461046603815184547448500356709747751240174841833572042746076994688103762014887289093164358500713894649408941442423277422140921157781154756929793244704749906604162103591905158114452749907312989913112273170187103589915NCBITaxon:37296103609833418710491130798133679531640729946710340157863033373306383011306413992530642363650591169654788546981343171033729940445862073401153571119529652611745531114716133865413131663430552132312017983602532371255232993512993850573163176426197940115134584457726787286232193348621160791392227983516811961255228149539322116761757312108077213487999948861814084341033519963218496209913924882097209620953304393755699286266447NCBITaxon:2697049339862436941103121958832050920290610997586448643249952529724674115834389427863789426256737863912334355141998349317998686554055343529148654514712454661042986357124679151641358884019373345087718238039129039129529719748693102868899951337310548771251632138985076255470119380625631886571303443516558658862238NCBITaxon:44664946454636493NCBITaxon:11071142800624212428835649137514151678458810874391459438543311167810778543144080439334NCBITaxon:1108295346939773821899318226900351283871459531168853399669527869595418694200308954486972443662066563954530055862335518042643086922003022848124264281504751499105102991029841445238626290319541835120895574974311700893062186441930615755841310165382352557722399463601066016236010439947544404237610212790568813377518236355721162296298176771870931281105023824518557966946543186320518240974417394493235124501288531838891988531911137825591037164735166012615572283313NCBITaxon:10114284503163119857385952396939343237956026087121346395984275295952664373343232235443142752422430814336195931334565943274169963112223360520121697952528386972864368012187073347472790104081882976038181249885340188439733127441416395888587252029554648038343691224326408172124193559552128161405592042546551945822886091838272759212744234081701933298297212744201274426235455552857943284139961660187625482964718762474801199150956481882297254317610121480154120455138473848188294519069515030341663360364849850569310151052835352835269373529454052018649729722258594109767745180481231006191107932271027943447256848479291146387033384991722617561094616143512032581205136521850523525944732054937347446644991759189158258038804333024480461206781122026049910806124619629022246197320637522991867051186705750906812193879118794739800718680157230746835741258117571508476918388580329031160861138886915216778672127399784459724751295139870435126246969281239771333399510343048138178182198131882682108022815610054762411229265716329571922221979116938197912124714854513283882813951080230294911040145954161934139036312041626658903264460711210411706127443210859562102694128316669436945597299495141065818790101058413308794784475174150808206542317447127070996428141119466914545869537932920101981264690410661145755139251923239232014081576973160621213312383911283128212803395228110108328252535315285128112353153498257419481478753623142410135674295105242619145481NCBInr 2013051911506212945912928299633941346959753121170519351626539003098006978103690188532256601736548566156641624251036951187023483348246882211870563863222618612992501646860688565651262321721956581010284283865671713604160235870015048022160100298823578613252911187002157641809699756655666452467223926291613633666336721751117645592321870933114767468342808NCBITaxon:40559235909322646523652621263374982178707498216347256569156925693872249821165448720569734507699300166690510894491089448NCBITaxon:2759661410NCBITaxon:493265358715568952071089444100058965508732287535135248126373006930398725121177687268723872487296549872724223130554417725784541581419198822445446117467310410590988446899601677674468587529600NCBITaxon:5811118226370824855784578458194439158059656636164034624136810893161001428099612NCBITaxon:548681NCBITaxon:58205464143945033077522836373791966272905896059606875944670875622708626659649604323259335483309029829387834810626756726756643061512793654319474704721917016108961548313969169287827573640782112931457075298352061892577712850248048541898548771587796273002515630415286964696439644491146247274612874601134506963852638526393024095264110909467469416348112386935231NCBITaxon:5659955767841195623305917365822573132697969903461344346360644042134511249515109760616125732924620096692729422729435133896858355943146968912801796811186961678078118694143618311869817194759321718118691443906NCBITaxon:102981257118387271566338404330618596948364190802703847664257310568708618218678661833568851350NCBITaxon:7803906NCBITaxon:150299031645151694312970298207621158612184519307991374209285201319747078529507619212638543915383379NCBITaxon:10239NCBITaxon:714173613095705470448300641751585923890037159084128108406972282334575412NCBITaxon:1024528104NCBITaxon:102433920174810977970671356225833136185509924286706527796583342409069102631034809NCBITaxon:63124067413076707070748761381080492672947472768917812126719285321773846451772177437104905615348119922512153239576769014NCBITaxon:7227156974726647091317047202697130327697859628596341147613091308130712638712120353326483731532146933832341147958370131413134937608333413118333357047111497039655397283332101841833312146874550451080187492002627241530091076620350541149028014851750130373983NCBITaxon:11552398813992743562359311792866839061194599160329328534229998103365786185336583517457428316273121536024271831627713519948875058NCBITaxon:1040731627524271550277916797181349130627450278029159541264432392915866002557021272113558867551229658793094520312410515192843435815398580156269811825902471494560990690272560272563354422841013356263416639286757227901NCBITaxon:5796537457574011233523562355522221045775126216131091575596915429310136413962242139213901857323271489623074116402784896160106757445802402512296833529441393665814036657625763206672400540296543NCBITaxon:117092823177298184318344322622469765755499229575757592845935561827066714367334442403531849223112105421152763910969762261111795752981882633184319229438152764098662652810442179626782531751327804267608565050176946996NCBITaxon:1313171250389427955880119873251979596214114082241408223264561421971014122851867631854315207677376777421926129454458057041744726724042649329NCBITaxon:17732088932088952075591011766455315231663367451011613284262658721392696358895423019531882915819883919436612336814498224911554382641310337998666940068845421575353115104298315468911129859356253232273075660469136165105612149165238976336316812149159704126308213494091328881885513613NCBITaxon:1865381889083264241916073NCBITaxon:18653662101736604315750393765573111623427306855743680863772166689NCBITaxon:138305959755284003197291761608994291703002731052311009040546465322432327605683406217860610645391982159739757441994742757758042277791955249131366239407313001464076204104411117086937451547922119856119857299204072NCBITaxon:5920165636713926767114501344084408797781074016269941279131478196794408140823803945281353403816596688722926123517861624323124323013599235612710876717978540964097978893052432776279129249115574840904092409344713512025316376163317831362538865856944155401813397174168709796628773239669202979362899315634301922344713763433872227954866343662821736231259248586110921386388420493211354724847947314473116968111290255161398128481317333672214407721316931555778122152250255183610036NCBITaxon:11266NCBITaxon:4394888485145494137499010041715916922596345910659213334991065901249615334542NCBITaxon:6622432754427124327421340112390171657167849641028968833329549124966842528265215810943432652157716019712790857162NCBITaxon:103572702NCBITaxon:103581194881654360507601435590NCBITaxon:10359170721272634185934783413223438022262762618903641969471825286349559839642711913645272621272623272624116071911771891238993121410246052312189481821314269704940324803113180803049031417981305212483431114778749093238481437113153688918413684174940804980404342714918256136491154373413156713164163159403021009714629772823525675060189519295214505111524962748492496731460519518766260201153348580021538186256226912328013603275614231422275119030491102214505204932101755275914282664179906914174609111020594082511211143919042762167539131463977851866139077527142735071061981353008559292148691714511414448340732036962232834959NCBITaxon:1167612296211634342375451367830495237109458015802362725315396