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KhanMass SpectrometryShotgun proteomicsWounds And InjuriesNot availableHypocotylProteomicsSoybeanFloodingRecoveryhttp://www.ebi.ac.uk/pride/archive/projects/PXD001567Plant CellHypocotylThe liquid nitrogen frozen hypocotyls were homogenized in acetone solution containing 10% trichloro acetic acid and 0.07% 2-mercaptoethanol, and proteins were extracted following the standard protocol. The extracted proteins were purified with methanol and chloroform to remove any detergent from the sample solutions. The purified samples were digested with trypsin and lysyl endopeptidase, then desalted and acidified in formic acid before applying to nanoLC-MS/MS. A nanospray LTQ Orbitrap mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) was operated in data-dependent acquisition mode with the installed XCalibur software (version 2.0.7, Thermo Fisher Scientific). Peptides in 0.1% formic acid were loaded onto a C18 PepMap trap column (300 μm × 5mm) (Dionex, Germering, Germany) of an Ultimate 3000 NanoLC system. The peptides were eluted from the trap column with a linear acetonitrile gradient (8-30% in 120 min) in 0.1% formic acid at a flow rate of 200 nL/min. The peptides eluted from the trap column were separated and sprayed on a C18 NANO HPLC NTTC-360/75-3 capillary tip column (75 μm ID × 120 mm, Nikkyo Technos) with a spray voltage of 1.5 KV. Full-scan mass spectra were acquired in the LTQ Orbitrap mass spectrometer over 400-1500 m/z with a resolution of 30000.PrideGel-free quantification methodiodoacetamide derivatized residuemonohydroxylated residueProteins were identified using the Mascot search engine (version 2.4.0.2, Matrix Science, London, UK) of a soybean peptide database (73,320 sequences) constructed from soybean genome database (Phytozome version 9.1, http://www.phytozome.net/soybean). The acquired raw data files were processed using proteome discoverer (version 2.3.2), and the parameters used in Mascot searches were as follows: Carbamidomethylation of cysteine was set as a fixed modification and oxidation of methionine was set as a variable modification. Trypsin was specified as the proteolytic enzyme and one missed cleavage was allowed. Peptide mass tolerance was set at 10 ppm, fragment mass tolerance was set at 0.8 Da, and peptide charge was set at +2, +3, and +4. An automatic decoy database search was performed as part of the search. Mascot results were filtered with the Mascot percolator package to improve the accuracy and sensitivity of peptide identification. For differential analyses, a commercial label-free quantification package, SIEVE software was used for comparing the relative abundance of peptides and proteins between control and experimental groups. The chromatographic peaks detected by MS were aligned and the peptide peaks were detected as frames using the following settings: frame time width, 5 min; and frame m/z width, 10. Frames were generated for all parent ions scanned by MS/MS and were matched to exported Mascot results to identify peptides. In the differential analyses, total ion current was used as a normalization factor.ProteomicsSetsuko KomatsuLTQ XLNational Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, Tsukuba 305-8518, JapanPARTIALGlycine Maxmnkhan@affrc.go.jp25818724 Khan MN, Sakata K, Komatsu S. Proteomic analysis of soybean hypocotyl during recovery after flooding stress. J Proteomics. 2015 May 21;121:15-27 10.1016/j.jprot.2015.03.020BiologicalUniversity of TsukubaSoybean is a nutritionally important crop, but exhibits reduced growth and yields under flooding stress. To investigate soybean responses during post-flooding recovery, a gel-free proteomic technique was used to examine the protein profile in the hypocotyl. Two-day-old soybeans were flooded for 2 days and hypocotyl was collected under flooding and during the post-flooding recovery period. A total of 498 and 70 proteins were significantly changed in control and post-flooding recovering soybeans, respectively. Based on proteomic and clustering analyses, three proteins were selected for mRNA expression and enzyme activity assays. Pyruvate kinase was increased under flooding, but gradually decreased during post-flooding recovery period at protein abundance, mRNA, and enzyme activity levels. Nucleotidylyl transferase was decreased under flooding and increased during post-flooding recovery at both mRNA expression and enzyme activity levels. Beta-ketoacyl reductase 1 was increased under flooding and decreased during recovery at protein abundance and mRNA expression levels, but its enzyme activity gradually increased during the post-flooding recovery period. These results suggest that pyruvate kinase, nucleotidylyl transferase, and beta-ketoacyl reductase play key roles in post-flooding recovery in soybean hypocotyl by promoting glycolysis for the generation of ATP and regulation of secondary metabolic pathways.<h4>Biological significance</h4>This study analyzed post-flooding recovery response mechanisms in soybean hypocotyl, which is a model organ for studying secondary growth, using a gel-free proteomic technique. Mass spectrometry analysis of proteins extracted from soybean hypocotyls identified 20 common proteins between control and flooding-stressed soybeans that changed significantly in abundance over time. The hypocotyl proteins that changed during post-flooding recovery were assigned to protein, development, secondary metabolism, and glycolysis categories. The analysis revealed that three proteins, pyruvate kinase, nucleotidylyl transferase, and beta-ketoacyl reductase, were increased in hypocotyl under flooding conditions and during post-flooding recovery. The proteins are involved in glycolysis, nucleotide synthesis and amino acid activation, and complex fatty acid biosynthesis.Proteomic analysis of soybean hypocotyl during recovery after flooding stress.Khan Mudassar Nawaz MN, Sakata Katsumi K, Komatsu Setsuko SMethane, sodium salt, Nup32D, ammonium formate, 13C-labeled, PLIP, cadmium salt, MeCN, NCMe, Vinegar, zinc formate, atado, Essigsaeure, lead salt, magnesium formate, zinc salt, Type 2A-interacting protein, trichloro-, thomson., 2-Propanone, peptide, Polypeptides, Ethanol, ammonium tetraformate, E260, peptido, Glacial Acetic Acid, cobalt(II) formate dihydrate, dJ69E11.3, Detergents, CH3-C#N, B1, Min, Software Engineering, Computer Program, WMS, DmelCG42273, HTATIP, wood alcohol, nup154, 2810441M03Rik, Divorced, peptides, N, Open, Acetic Acid, 2 Mercaptoethanol, Computer Programs and Programming, min, achromobacter beta-lytic protease (blp), mAPC, proteins, Divorces, CG12352, Acetic Acid Glacial, copper, SCAN, HPLC, AI047805, hNAT5, lithium salt, hSAN, cPLA2, column, sample, D1, MeCO2H, n, ethoic acid, blood capillary, Sodium Methoxide, GPHYSD2, TIP, ammonium (2:1) salt, DmAAF34715, nickel salt, Mak3p, SGS, Alcohol, NAT13, cobalt (+2) salt, ZC2HC5, detergent, Dmel_CG7826, Th, HTATIP1, tip, Nat13, ANAC091, not genetically inherited, Source Softwares, Software Tools, Programs, ACMICD, Program, Computer Applications, Putative MAPK-activating protein PM10, Glacial, sodium (4:1:1) salt, Stickstoff, magnesium salt, Dm1, Computer Applications Software, lysyl endoprotease, Computer Applications Softwares, Hypocotyls, Softwares, TIP60, nickel (+2) salt, Carbinol, Acetic acid, Dmel_CG7835, carbinol, Mnb, MNB, ammonium salt, Software Applications, Ethylic acid, Source Software, formate, cobaltous formate, cromium (+3), MASS, AW124434, study protocol, beta Trypsin, Trichloromethane, organ system, METHANOL, nanospray, Wood, Applications, nitrogen, spirit of wood, 2-Mercaptoethanol, copper salt, ammonium (4:1) salt, Polypeptide, methyl cyanide, 7N, lead (+2) salt, tlp, TIP41, 2600005K24Rik, Methyl Alcohol, Computer Software Applications, 2-ME, E-260, nat5, nickel formate dihydrate, lead formate, aluminum salt, cesium salt, FBN, CH3-COOH, Gene, Methyl, NAT5P, CH3OH, Computer, NAT13P, Methylalkohol, body system, CG7826, ESA1, formic acid, mass-to-charge ratio, Methanecarboxylic acid, method, potassium salt, ECTOL1, 14C-labeled, method used in an experiment, Arabidopsis NAC domain containing protein 91, Gene Products, CG7835, CG42273, zk, Wood Alcohol, system, nat13, Separated, CH3CO2H, Application, Open Source Softwares, l(2)10432, anatomical systems, thallium (+1) salt, Software Application, beta-Trypsin, rubidium salt, Open Source Software, methanol, calcium formate, methanoic acid, Solution, Computer Software Application, OCTD, DmelCG4579, ms(2)zk, Tools, wood naphtha, DmelCG12352, Nat5, NAT5, peptidos, liquid, cromium (+3) salt, l(2)01501, sodium formate, PTHB1, span, Applications Software, Open Source, AcOH, data, DYRK1, Computer Software, nickel formate, 1-(14)C-labeled, 3H-labeled, BcDNA:LD21772, mak3, Proteins, strontium formate, Methyl alcohol, potassium formate, copper (+2) salt, strontium salt, acetonitrile, Peptide, Methanol, Tool, polypeptide, AW112078, Software Tool, capillary vessel, HOAc, C18, cupric formate, Protein, MFS1, Sodium, MeOH, Dyrk1, connected anatomical system, aluminum formate, Software, ACETONITRILE, cyanomethane, WMS2, Achromobacter protease I, San, SAN, AU020952, Separation, 10^[-9], nup32D, lithium formate, E 260, Separations, CC1, Engineering, Tripcellim, CG4579, acide acetique, azote, ethanenitrile, sample population, chromic formate, nitrogeno, ME-IV, Protein Gene Products, plan specification, achromopeptidase, san, Computer Programs, Gene Proteins, Trypure, Applications Softwares, MeCOOH, ACETIC ACID, wood spirit, SSKS, 2-mercapto-, calcium salt, TCV-interacting protein, INS No. 260, Peptid, acetic acid, Mak3, Ethanoic acid, Methoxide, MAK3soybean, cv. Wye, Phaseolus max, Glycine max, proteomic analysis, soybeans, Hypocotyls.Bru, IPP2A2, Raw, ion, NetrinA, D430049E23Rik, cleavage, Long Term, 5730420M11Rik, peptide, Polypeptides, L-Isomer Methionine, Methionine, Drug Tolerance, ClvPrd, peptido, Min, Software Engineering, Effect, WMS, Computer Program, DmelCG42273, C79691, Svc, SET, Elkh, peptides, reference sample, Genomes, TAF-I, M, phosphatase 2A inhibitor I2PP2A, Open, EK6, Computer Programs and Programming, min, mAPC, proteins, Sap-2, free, Lccp, DmelCG4299, AI047805, allergic reaction, neuroendocrine tumour, set, IGAAD, Solute carrier family 6 member 2, DmelCG10574, ppm, CT27014, NET1, SLC6A5, Half Cystine, netA, GPHYSD2, Racemethionine, Long-Term Effects, drug tolerance, SGS, phapii, Elk, ELK, Zinc Cysteinate, Neuronally-expressed EPH-related tyrosine kinase, immune system tolerance, T5E21.12, 2-Amino-4-(methylthio)butyric acid, Longterm Effect, Dmel_CG7826, StF-IT-1, Th, SAP2, Search, 9330129L11, not genetically inherited, results, Source Softwares, neuroendocrine neoplasm, Software Tools, ACMICD, Programs, Program, Computer Applications, Dm1, Ionen, Norepinephrine transporter, Computer Applications Software, Computer Applications Softwares, Immunologic Tolerance, EPH-like kinase 6, Methionin, methionine, Hmet, Softwares, Dmel_CG7835, T5E21_12, Mnb, MNB, Software Applications, HLA-DR-associated protein II, DI-2, Phaseolus max, Source Software, I-2Dm, MASCOT, L-Cysteine, CG4299, MASS, hEK6, inhibitor of granzyme A-activated DNase, AW124434, beta Trypsin, experimental procedures, I-2PP1, cv. Wye, DmelCG18657, Applications, TAF-IBETA, Self Tolerance, Tolerance, hypersensitivity reaction disease, TAF-Ibeta, Polypeptide, i2pp2a, Liquimeth, time, Computer Software Applications, AW488255, breadth, Cysteine Hydrochloride, 2-amino-4-(methylsulfanyl)butanoic acid, experimental, AW549739, Effects, L Isomer, FBN, Gene, Computer, thomson, aligned, PHAPII, CG7826, netrin, mass-to-charge ratio, period, template-activating factor I, Hek6, ECTOL1, sensitive, Gene Products, CG7835, L-Methionine, CG42273, Cek6, Del(8)44H, Half-Cystine, sensitivity, Pedameth, Application, ENSMUSG00000074119, Erp, APUDoma, ERP, Open Source Softwares, Search Engines, methods, Longterm, L Cysteine, Software Application, experimental section, iones, soybeans, ipp2a2, Tyrosine-protein kinase receptor EPH-2, beta-Trypsin, Open Source Software, 2pp2a, Sciences, Long-Term, ions, CG10574, Computer Software Application, soybean, OCTD, mKIAA0989, 2.7.10.1, 2PP2A, 2-amino-4-(methylthio)butanoic acid, 10^[-6], Tools, taf-ibeta, Etrp, dSET, dSet, peptidos, NAT1, hypersensitivity reaction, Long-Term Effect, metionina, Net, NET, Controlled, Applications Software, Open Source, data, Controlling, DYRK1, C130099E04Rik, Computer Software, DL-Methionine, Proteins, EPH tyrosine kinase 2, igaad, aligned to, neuroendocrine tumor, L-Isomer, net, Peptide, Engine, Tool, polypeptide, Software Tool, TIC., Immune Tolerance, I-2PP2A, Protein, Dm I-2, Long Term Effects, I2PP2A, MFS1, Dyrk1, Software, Data Base, Met, WMS2, AU020952, hypersensitivity, hypersensitive, Col4a-1, CC1, Engineering, Tripcellim, CG18657, Longterm Effects, ME-IV, Protein Gene Products, Computer Programs, Gene Proteins, Trypure, dSET/TAF-Ibeta, 2610030F17Rik, Applications Softwares, Ion, EPHT2, Glycine max, SSKS, alpha-amino-gamma-methylmercaptobutyric acid, Peptid, AA407739, netrin A, Immunological Tolerancesodium salt, Hypochlorous acid, Antiformin, postnatal development, growth and development, Visible Light, CG11121, Ly113, period, profilin, surface, old, Caucasian, Abc8, DmelCG11121, Sodium Hypochlorite (Solution), hnu, light, Elaiosome, whitish, foton, integumentum commune, l(2)27/7, umol, D88, somda, CG9553, Soybean, Lichtquant, soybeans, Soy Beans, Plant Embryo, Zygote, Visible, SO, Solution, soybean, µmol, Diaspores, Humidities, Clorox, photon, Zygotes, Beans, GH4, Quartz (SiO2), TR2, Soy, So, gamma, Chic, Drl, Soybeans, Elaiosomes, Chi, Bonin Islands, Radiation, external covering of organism, AW413978, white, time., Hypochlorite, organism surface, Light, CD258, Mdu, Soy Bean, development, chi, LIGHT, mda, Embryos, sand, Hypocotyls, Sodium, Seed, ami, Hydrogen Oxide, Visible Radiations, Radiations, Embryo, Diaspore, Visible Radiation, HVEML, growth pattern, Phaseolus max, body surface, HVEM-L, non-developmental growth, med, Bean, postnatal growth, Photoradiation, Plant, Plant Zygote, Plant Embryos, DmelCG9553, LTg, cv. Wye, light quantum, Photoradiations, Plant Zygotes, integumentary system, Whites, Glycine max, Sands, White, dermal system, growth, timesingle-organism developmental process, determination, Metabolisms, postnatal development, LUC7A, DELTA AND GAMMA, Embden-Meyerhof, growth and development, protein, betaTub1, Embden Meyerhof Parnas Pathway, Long Term, CG11121, element, secondary metabolite metabolism, protein polypeptide chains, B1t, old, responsivity, DGS, Embden-Meyerhof Pathway, protein aggregate, present in fewer numbers in organism, Effect, IKKg, KEY, Key, organ, increased, kus, reference sample, beta1tub, CG9277, Soybean, Soy Beans, nucleotide formation, hypoplasia, T, beta-particle, Pathways, proteins, Pyruvate Kinase L, free, SO, 1t, e, ATPsyn b, decreased, L-Type Pyruvate Kinase, e-, L-Type, beta1Tub, DmelCG1106, nucleotide synthesis, Long-Term Effects, So, ATP, ATPsyn-&bgr, Soybeans, BETA 56D, Pathway, ATPsyn-b, CROP, Crop, fatty acid anabolism, anaerobic glycolysis, Longterm Effect, Embden-Meyerhof-Parnas, results, Soy Bean, Embden-Meyerhof-Parnas Pathway, DmIKKgamma, L Type Pyruvate Kinase, l(1)G0108, Playthings and Play, dIKK, Kenny, Plaything, Hypocotyls, FLOWERING TIME CONTROL PROTEIN FCA ALPHA, secondary metabolism, CG11154, beta-tub, protein_coding_transcript, Beta <eudicots>, growth pattern, Phaseolus max, craw, non-developmental growth, CREAP-1, IKK-gamma, Toys, FCAALL.331, common, beta56D, DmelCG9277, M-Type, Embden-Meyerhof pathway, CG1106, M1-Type Pyruvate Kinase, beta1-tub, beta(-), DTB2, cv. Wye, beta-Tub, metastatic, DmelCG16910, glycolysis, i6, DL4180C, M2 Type Pyruvate Kinase, fatty acid biosynthesis, beta-Tub56D, Embden-Meyerhof Pathways, time, accessory, betaTub, Specbeta, beta1-Tubulin, hLuc7A, secondary metabolite metabolic process, R-Type Pyruvate Kinase, Plays, Effects, e(-), fatty acid formation., Gene, electron, IB, protein-containing complex, supernumerary, dIKK-gamma, period, Transferase, reduced, polypeptide chain, B-spec, DmelCG11121, subnumerary, betaspec, Metabolism, DmIKK-gamma, ATP-synbeta, Gene Products, tiny, dmIKKgamma, IKK[[gamma]], RGD1307981, Toy, study, reactivity, nucleotide anabolism, somda, betatub(56D), Playthings, DmelCG11154, Longterm, soybeans, gel, Elektron, beta[[1]] tubulin, beta-ATPase, beta1t, messenger RNA, Long-Term, decreased number, M Type Pyruvate Kinase, soybean, anon-EST:fe1B3, template RNA, Puppets, IKK, ATPasebeta, SPEC8, anatomical unit, ATPase beta, body organ, Play, Beans, Long-Term Effect, fatty acid synthesis, CG5870, R-Type, ATPB, Soy, M1-Type, modifed Embden-Meyerhof pathway, Puppet, Controlled, Drl, small, cambial secondary growth, beta-tubulin56D, Spec-beta, M-Type Pyruvate Kinase, Controlling, Dmbeta1, Embden Meyerhof Pathway, nucleotide biosynthesis, protein complex, OA48-18, Proteins, Tubulin, enzyme activity, M2-Type, i168, Mdu, Pyruvate Kinase, ATP-syn-B, Secondary Metabolisms, development, IKKgamma, ML-1, native protein, natural protein, mda, M1 Type Pyruvate Kinase, Protein, Long Term Effects, chemical analysis, INSDC_feature:mRNA, betaSpec, CRA, b-Spec, M2-Type Pyruvate Kinase, ami, DmelCG5870, l(1)G0074, l(1)G0198, negatron, underdeveloped, mRNA, beta[[1]]-tubulin, Dmikkgamma, med, R Type Pyruvate Kinase, increased number, Bean, postnatal growth, b spectrin, beta1, CG16910, Embden-Meyerhof-Parnas pathway, beta, Longterm Effects, Protein Gene Products, Tub, Gene Proteins, present in greater numbers in organism, Secondary, beta-spec, Glycine max, ATPIB, BETA, regulation, assay, response, growth, Specsoybean, cv. Wye, assay, determination, Peptidomics, Phaseolus max, Glycine max, chemical analysis, soybeans, Hypocotyls.380trueProteomics Analysis of Soybean Hypocotyl during Recovery after Flooding StressSoybean (Glycine max L. cv Enrei) seeds were sterilized with 2% sodium hypochlorite solution and then thoroughly rinsed in water. The sterilized seeds were sown 4 cm inside surface of sand in 450 mL of quartz seedling cases wetted with 150 mL water and grown at 25°C and 70% humidity in a growth chamber (Sanyo, Tokyo, Japan) under white fluorescent light (160 μmol m-2 s-1, 16 h light period/day). Two-day-old soybeans were flooded for 2 days and hypocotyl samples were collected at the day of removal of flooding and then at 2 more points during the 4-day recovery period.2015-05-262014-12-03PXD00156728065516460991804982953585111454224942024527872842188090368093270429330845811363336351524260119834300662758385NCBITaxon:128068888561896111430118726355557114526236493461364031952065126957366649274670738538836411354615821582310665688954762721123294422291180992323122586365458333460792519365914320597129386558195851149315242592637371250232NCBITaxon:127211195774818661488584310430024513586722175543740816918045426683429531945305861266835149822505814963674452158554528113208147956410039237514636942049433208964208963445974137979138712728384400667453293529367156759388036478434716052165994048321659721659545501315282693042165915627161696833218321729204277944762376454317203094980197525556978159509439843948840479NCBITaxon:2157525338551531051067439899037122857759515205968762262311210884555588845587077918178081736309431794571698936160488409859NCBITaxon:3716210478267940150590714836432042106132049138169363035554320466326333119945654535110254111032174592161292642012886764100264203694569410167634102107612773515248671316582392547279334281461358430141729876091746334577444916754760489321287356756840301350621917265181301941119294393671104113102393202555986325827883965910475934749516049016529983552566317585439801102432608663102459808757417555183201964471452680457966313670190832331156114155355104219325552113834397634981812359871069955529NCBITaxon:4751850298335476113615741465477180066556484337677299767982510216998238976484074137351155711089725482444474153NCBITaxon:505578996984410003646534933414714749354801946751299114359741276473137086326547876565893639835322159589366983819201110677577271034819852348780265554479464098609958615718717668599191572951372211711012929210768783497419860202809148840633424923423515233598598865597464998703920211107294331171139363527215987419085037117082711708284182350241451139692931902197822474636046147123749402375939723287581722532566489050794526591972211915853101774208588596NCBITaxon:615750521028307989515586411051989972375059576131720002634196102641088878176179212190659407121906772275885815689964975151950582873757244NCBITaxon:22023717NCBITaxon:17036122019998570506050617476792745062724915961533355418400524643213686150596487844612286662941786661347515363291018194301086731018510184NCBITaxon:1763573039635586331492862876705801321650852718481865368595105135553482260710185687294296110091416333NCBITaxon:619184023210007348824123363630710470058127187878645159812894555663577553470951451225786425011260704NCBITaxon:13356262607052607076584574054163677483167233150561007133705654195NCBITaxon:305550445791399878168119011928752829126806315327341478281055524NCBITaxon:9606135858469008111167815022830519302836728593008529005NCBITaxon:569382688306401794699151011849943525875913151590144824283811139442258441604193704759272370860423281677285035158199011951953267386370137024924053608838581622488186156227748817562615835583715333760572671193501998086119350216787910857913711371272407190650371475917262981288057125627457572709030903113335503728142433747924910078711082NCBITaxon:96157243359463318586260799141262200298492670NCBITaxon:1263719281249212528303856838569591169266100658137352375612750454266625515197881356638375064422336009412934979054632245352472140125751511649908515151438992374537474454464152432359376059202228870515805955365550881579327160293851578114978633892894535936479437NCBITaxon:68544127542251177529915901090134167387158955210129661030612967158515845158493765939343271591002263767338899046142345128768959119833296646704146479293657616428034116022164133159928038280353690910891768814989462181214108034817939228788929502711313329528282944568617493468191126526997227321890382107601247190400727159736997828114311402064119810871355477436581424507229533796982042321242231291632180334708714537209164573321535815754699287770202060328114319338293550833291292211486437457252649997283035739087183087267271037908550717629970767892683704155737292140532941281607839563924314262654898227377561155962073220865958827355516936053307972551811354914321371597495350261159556143213840607258781414471509493349194963401614334728005936046316461046603815184547448500356709747777195124017484185560133575554792042741580536076994688103762014887100594120037582890931643542826885007123038399001389464940838283594144242322197857742171598921409212205853939621157781154756929793244704749906604162103591905158114452749907312987779913112273170187546991103589915NCBITaxon:37296103609833418710491130798133679531640729946710340157865826453033373306383011306413992530642363650591169654788546981121162034317652616103372861299404458620734011535711195296526117455311147161338654131316634305521323120179836025323731276125523299351299385057316317642619794011513458445772678728623219334151346186208621160791392227983516811961255228149539322323116761757312108077213487999948861856962814084341033517787996321849620994887321392488209711533820961153392095330439375569927612686266447NCBITaxon:2697049339862436941103121958832050920290610130685999758644458828643249952529724642606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