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/PXD002167/v130422v05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130522v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130503v04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130526v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130527v01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131108v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131104v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131105v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130429v05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130508v05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131113v04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130526v04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131106v04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131028v02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131108v04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130523v01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131030v05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131107v01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130517v05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131101v01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131105v01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130513v05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131112v02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131111v02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130517v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130518v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131113v02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130510v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130513v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130514v03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v131111v04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/v130423v01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/SEARCH_MAXQUANT.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/OTHER_MIAPE_PXD002167.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/OTHER_FASTA.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/OTHER_MATHEMATICA.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002167/OTHER_EXCEL_FINAL.zipprimaryOK200003890gillespp@ohsu.eduPeter Barr-GillespieMass SpectrometryShotgun proteomicsNot availableStereociliaHair bundlesAuditoryHair cellsKinociliaVestibularhttp://www.ebi.ac.uk/pride/archive/projects/PXD002167UtricleInner EarVestibular Hair CellWe collected four sets of samples, including purified mouse hair bundles from postnatal day 4-6 mice (referred to as "P5") and P21-P25 utricles ("P23"), as well as utricular epithelium sensory epithelium from the same ages. Four biological replicates were prepared for each of the four conditions. Proteins were partially separated by SDS-PAGE, then digested into peptides. LC-MS/MS was used to identify and quantify peptides from the four conditions.PrideNot availableiodoacetamide derivatized residueWe used MaxQuant, with its built-in Andromeda search engine, to match peptides to mouse protein database entries, to assemble peptides into proteins, and the quantify those proteins.ProteomicsPeter Barr-GillespieLTQ Orbitrap ElitePARTIALOregon Hearing Research Center Oregon Health & Science University Portland OR 97239 USAMus Musculus (mouse)27251877 Krey JF, Drummond M, Foster S, Porsov E, Vijayakumar S, Choi D, Friderici K, Jones SM, Nuttall AL, Barr-Gillespie PG. Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and Function. Sci Rep. 2016 Jun 2;6:27221 10.1038/srep27221gillespp@ohsu.eduBiologicalOregon Hearing Research CenterUnited StatesThe phospholipid- and Ca(2+)-binding protein annexin A5 (ANXA5) is the most abundant membrane-associated protein of ~P23 mouse vestibular hair bundles, the inner ear's sensory organelle. Using quantitative mass spectrometry, we estimated that ANXA5 accounts for ~15,000 copies per stereocilium, or ~2% of the total protein there. Although seven other annexin genes are expressed in mouse utricles, mass spectrometry showed that none were present at levels near ANXA5 in bundles and none were upregulated in stereocilia of Anxa5(-/-) mice. Annexins have been proposed to mediate Ca(2+)-dependent repair of membrane lesions, which could be part of the repair mechanism in hair cells after noise damage. Nevertheless, mature Anxa5(-/-) mice not only have normal hearing and balance function, but following noise exposure, they are identical to wild-type mice in their temporary or permanent changes in hearing sensitivity. We suggest that despite the unusually high levels of ANXA5 in bundles, it does not play a role in the bundle's key function, mechanotransduction, at least until after two months of age in the cochlea and six months of age in the vestibular system. These results reinforce the lack of correlation between abundance of a protein in a specific compartment or cellular structure and its functional significance.Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and Function.Krey Jocelyn F JF, Drummond Meghan M, Foster Sarah S, Porsov Edward E, Vijayakumar Sarath S, Choi Dongseok D, Friderici Karen K, Jones Sherri M SM, Nuttall Alfred L AL, Barr-Gillespie Peter G PGliquid chromatography tandem mass spectroscopy, Epithelial Tissues, setulae, Cyclin-dependent kinase inhibitor 1A (P21, 1110014C03Rik, WEX9, NCK5A, Laboratory, pp21, HSP 25, Mus domesticus, 21 kDa polypeptide, CASP-14, SDH, p21[dacapo], House Mouse, p35nck5a, Histamine-releasing factor, Hair, peptide, SDS, Polypeptides, Epithelial Tissue, peptido, Ptges, HSP 27, Tau protein kinase II 23 kDa subunit, S31I125, neuroepithelium, Divorced, Fetal Hairs, Alpha(B)-crystallin, LCMSMS, peptides, SV-40-induced 24P3 protein, vibrissa, cPGES, P21 protein, C4, Tissue, E(Sev-CycE)2B, Swiss Mice, proteins, Divorces, Melanoma differentiation-associated protein 6, TEBP, p21WAF, D4S234, CG10846, SOLO DANCERS, Fetal, p16Xic2, Fortilin, p02, P15, house mouse, D4S234E, HRF, LC-MS2, Mesothelium, cip1, P21, P23, mouse, LC-MS/MS, P25, AI836084, Hairs, p24 family protein alpha-2, portion of epithelium, Mini-ICE, p21, p23, p25, p24, p27, l(2)br2, Tebp, CAP20, Mus musculus, whisker, Caspase-14 subunit p10, TPPP|p25, Tmp-21-I, mice, Swiss Mouse, Cdkn1, p35, Caspase-14 subunit p19, Cyclin-dependent kinase 5 regulatory subunit 1, CIP1, Growth-related 25 kDa protein, MICE, Waf1, WAF1, CDK5 activator 1, Crya2, Mesothelial Tissues, domesticus, CDKN2B, P24(DELTA), p27[Dap], Melanoma differentiation-associated protein, DS00929.1, Oncogene 24p3, liquid chromatography-tandem mass spectroscopy, MDA-6, TPPP1, Mouse, Polypeptide, BC011290, CDKN1, 3.4.22.-, F10B6_15, l(2)04454, DmelCG1772, sensory epithelium, CIB1, TCTP, Kras2, Gene, mini-ICE, 5730442A20Rik, F10B6.15, LC-MS-MS, cdi4, Major prostatic secretory glycoprotein, p21Cip1, p21CIP1, House, Alpha-2-microglobulin-related protein, Alpha-2U globulin-related protein, LC-MSMS, Gene Products, Mus musculus domesticus, Cdi4, CDI4, Lipocalin-2, Decapo, Mice, Separated, Lanugo, SDI1, Epithelial, Fetal Hair, S31III125, Swiss, Tissues, coat/ hair, 4733401P19Rik, CDKI, NEEP21, Dac, quill, dacapo/cyclin-dependent kinase interactor 4, 21 kDa transmembrane-trafficking protein, Crya-2, DmelCG10846, Dap, peptidos, Mesothelial, TPPP/p25, Transmembrane protein Tmp21, CDK-interacting protein 1, NGAL, fur, D11Bwg0379e, Proteins, Trt, CDK5R, c-Ki-ras, Siderocalin LCN2, l(2)35Bf, p24alpha2, Peptide, Cyclin-dependent kinase 5 activator 1, p24delta, polypeptide, Cip1), Tmp21, LC/MS/MS, Mus, Protein, CG1772, HSGP25L2G, HspB5, TMP21, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Cdk5r, HspB1, p24 family protein delta-1, TPKII regulatory subunit, Protein Cdkn1a, p24delta1, sid3177, Heat shock 25 kDa protein, Mesothelial Tissue, Separation, BG:DS00929.1, Glycoprotein 25L2, epithelial tissue, Separations, 0610011M09Rik, hair, SWDS, House Mice, SIIR, CGI-97, microchaeta, Laboratory Mice, SBP, Protein Gene Products, GMP25, CDK5P35, Gene Proteins, br2, liquid chromatography tandem mass spectrometry, Peptid, coat hair, AV082259, AV082137, Heat shock 27 kDa protein, Laboratory Mouse, Peptide., p25alpha, mda6fur, setulae, membrane, Stereovillus, transmembrane, membrane of organ, whisker, Fetal Hairs, Fetal Hair, single-organism developmental process, function., protein complex, coat/ hair, membrane region, vibrissa, Stereocilium, hair, integral to membrane, Hairs, proteins, Endonexin II, protein, membranous organ component, protein-containing complex, Stereovilli, microchaeta, Hair, development, Lipocortin-V, protein polypeptide chains, Calphobindin I, native protein, natural protein, polypeptide chain, quill, Fetal, integral component of membrane, Protein, region of membrane, whole membrane, associated, protein aggregate, coat hair, Lipocortin V, Annexin V, Anchorin CII, Placental Anticoagulant Protein I, LanugoSearch Engines, peptides, Protein., protein complex, mice, Proteins, mouse, Gene, proteins, Search, protein, protein-containing complex, Peptide, Engine, Protein Gene Products, polypeptide, peptide, Gene Proteins, Polypeptides, protein polypeptide chains, peptido, native protein, natural protein, polypeptide chain, Mus, Protein, Gene Products, Peptid, peptidos, Mouse, Polypeptide, house mouse, protein aggregate, Data BaseEpithelial Tissues, setulae, DmelCG5178, SCS, Cyclin-dependent kinase inhibitor 1A (P21, act 87E, DmelCG7478, WEX9, NCK5A, single-organism developmental process, Tandem, pp21, Ska10, HSP 25, 21 kDa polypeptide, Stereocilium, p21[dacapo], protein, Mass Spectrometry Mass Spectrometry, Act5, p35nck5a, Hair, l(1)G0420, DmelCG12051, protein polypeptide chains, act 88F, Epithelial Tissue, HSP 27, AA960487, protein aggregate, Tau protein kinase II 23 kDa subunit, A, rsd, C, D, E, Fetal Hairs, cyt5C, SV-40-induced 24P3 protein, M, vibrissa, P21 protein, C4, Tissue, E(Sev-CycE)2B, proteins, Mass Spectrometry, present in organism, Melanoma differentiation-associated protein 6, act88F, p21WAF, D4S234, free, CG10846, Fetal, act 42A, p16Xic2, Ac5C, CG4027, Act, bHLHa38, P15, house mouse, DmelCG18290, D4S234E, act87E, Mesothelium, l(1)G0330, cip1, Actin/BAP47, P21, mouse, P25, act, Hairs, act42A, p24 family protein alpha-2, ACTIN, portion of epithelium, AFFX-Dros-ACTIN_M_r_at, p21, p23, actin, p25, p24, p27, CG7478, CG5178, l(2)br2, CG10067, Act57A, CAP20, whisker, DmelCG4027, Xtwi, TPPP|p25, MS/MS, mice, l(1)G0117, Pde, Cdkn1, p35, Actin, Cyclin-dependent kinase 5 regulatory subunit 1, CIP1, Growth-related 25 kDa protein, beta-actin/Bap47, Waf1, Act57b, WAF1, CDK5 activator 1, Mesothelial Tissues, l(1)G0486, act79B, l(1)G0245, CDKN2B, actin5C, l(1)G0009, p27[Dap], Melanoma differentiation-associated protein, DS00929.1, Ska-lt-m10Jus-gt-, Oncogene 24p3, MDA-6, TPPP1, CRS1, Mouse, BC011290, CDKN1, act57b, pdt, Act5c, l(2)04454, DmelCG1772, l(1)G0010, Act87e, CIB1, Xtwist, TCTP, single-organism developmental process., Kras2, ACT5C, CG12051, Gene, protein-containing complex, anon-EST:CL2c12, act57B, CG30294, l(1)G0025, cdi4, act57A, Major prostatic secretory glycoprotein, method, Act88f, p21Cip1, p21CIP1, polypeptide chain, Alpha-2-microglobulin-related protein, method used in an experiment, Alpha-2U globulin-related protein, actin-12, Gene Products, Cdi4, CDI4, Lipocalin-2, Decapo, Lanugo, 143060_f_at, SDI1, Epithelial, act5C, Fetal Hair, DmelCG10067, Tissues, coat/ hair, MS2, mus79B, Act57, twist1, twist2, CDKI, anon-EST:fe2D2, Act42a, M-Twist, NEEP21, Dac, CG18290, quill, dacapo/cyclin-dependent kinase interactor 4, DmelCG10846, T10, Dap, liquid, T11, Mesothelial, TPPP/p25, Chromatographies, Act-5C, CDK-interacting protein 1, l(1)G0177, NGAL, Ifm(3)7, fur, 42A, data, D11Bwg0379e, act 79B, protein complex, Proteins, Trt, CDK5R, c-Ki-ras, BEST:RE38067, Stereovilli, Siderocalin LCN2, l(2)35Bf, p24alpha2, Cyclin-dependent kinase 5 activator 1, development, Cip1), native protein, natural protein, Mus, beta-actin, Protein, M32055, CG1772, Act42, HSGP25L2G, Vertebrate, Ska<m10Jus>, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Cdk5r, HspB1, TPKII regulatory subunit, Protein Cdkn1a, tandem MS, twist, X-twi, l(1)G0079, Heat shock 25 kDa protein, Mesothelial Tissue, Stereovillus, BG:DS00929.1, CRS, Glycoprotein 25L2, epithelial tissue, Act88-F, act 57A, TWIST, 0610011M09Rik, Twist, hair, act 5C, Mass Spectrometry-Mass Spectrometry, SIIR, microchaeta, mus87E, CSO, SBP, plan specification, Protein Gene Products, GMP25, CDK5P35, Gene Proteins, br2, Act(88F), BPES3, BPES2, BAP47, coat hair, AV082259, AV082137, Heat shock 27 kDa protein, Bap47, ACS3, Actin5C, p25alpha, mda6setulae, 1110014C03Rik, Materials, NCK5A, phospholipids, Laboratory, Mus domesticus, 21 kDa polypeptide, Stereocilium, A4, CASP-14, protein, Organelle, Chromobindins, House Mouse, Histamine-releasing factor, p35nck5a, MAGE-E1 antigen, Hair, Lipocortins, protein polypeptide chains, Roles, calcium(2+) ion, Cellular., Ptges, Concepts, PP4, Annexin IX, Analysis, protein aggregate, Tau protein kinase II 23 kDa subunit, S31I125, Annexin, Mass Spectrum Analysis, average, Cochleas, Alpha(B)-crystallin, Fetal Hairs, vestibular organ, Analyses, CALCIUM ION, membrane region, cPGES, vibrissa, Swiss Mice, proteins, present in organism, internal ear, TEBP, hearing, Estimated, Structures, allergic reaction, cochlear duct, cochlear part of bony labyrinth, Annexin X, Audition, Cellular Structure, Fetal, HCA1, cochleae, Role Concepts, Fortilin, p02, house mouse, associated, Lipocortin V, Annexin V, Annexin XI, HRF, vestibulomotor system, vestibular system, Noise, PLATEST, P23, mouse, Pollution, integral to membrane, Hairs, Normalcy, membranous organ component, Spectrum Analysis, results, doubly charged positive ion, Spectroscopy, Role Concept, Playthings and Play, p21, Mini-ICE, DAMAGE, Hepatocellular carcinoma-associated protein 1, p23, Role, auris interna, Plaything, p25, Genetic Materials, Cellular, Individual, Tebp, Genetic Material, Placental Anticoagulant Protein I, Mus musculus, membrane of organ, whisker, Caspase-14 subunit p10, Tmp-21-I, mice, System, Swiss Mouse, Spectrometry, Toys, p35, Cyclin-dependent kinase 5 regulatory subunit 1, Caspase-14 subunit p19, Ca(2+), MICE, Component, CDK5 activator 1, Crya2, domesticus, calcium, Health, P24(DELTA), DMDA, Cell Component, calcium(II) cation, Calphobindin I, Material, hypersensitivity reaction disease, vestibular apparatus, Cistron, Annexin VIII, Mouse, Noise Pollution, Calcimedins, labyrinth, mMage-e1, inner ear, Platelets, 3.4.22.-, Plays, TCTP, Components, number, Calpactins, Gene, 5730442A20Rik, mini-ICE, Vestibular Systems, Spectrum Analyses, protein-containing complex, equilibrioception system, Normalcies, presence, TYPE, Calelectrins, DAGA4, Ca2+, polypeptide chain, House, sensitive, integral component of membrane, Mass, Mus musculus domesticus, MAM, Mice, SCG3, vestibular organ system, Anchorin CII, sensitivity, Mass Spectroscopy, Annexin B9, permanent structure, Lanugo, Toy, Normalities, Phospholipid, Fetal Hair, Genetic, R74653, S31III125, Playthings, Swiss, coat/ hair, ligand, calcium(2+), Calcimedin, AI847422, Structure, Annexin A8, Calelectrin, perception of sound, ENX2, Puppets, quill, 21 kDa transmembrane-trafficking protein, Crya-2, region of membrane, Play, hypersensitivity reaction, Cell Components, Transmembrane protein Tmp21, Puppet, fur, Individual Health, membrane, protein complex, Annexin B10, Alpha-dystrobrevin-associated MAGE Protein, otocyst, Trt, CDK5R, function, Cistrons, HEL-S-7, Stereovilli, cochlear organ, Cell, Cyclin-dependent kinase 5 activator 1, LGMD2C, Concept, p24delta, Tmp21, count in organism, native protein, natural protein, Mus, Protein, lagenas, whole membrane, HspB5, TMP21, p24 family protein delta-1, TPKII regulatory subunit, Mass Spectrum Analyses, p24delta1, sid3177, a phospholipid derivative, Mass Spectrum, hypersensitivity, Stereovillus, transmembrane, hypersensitive, DMDA1, RGD1560259, Normality, Annexin A11, hair, ANX5, lagena, Anx5, House Mice, Endonexin II, Lipocortin, microchaeta, Noises, Laboratory Mice, RPRGL3, CDK5P35, Lipocortin-V, SCARMD2, Calpactin, quantitative, coat hair, Laboratory Mouse, presence or absence in organism, VestibularHair, fur, setulae, development, whisker, Fetal Hairs, Fetal Hair, Mus, quill, Fetal, mice, coat/ hair, single-organism developmental process., vibrissa, mouse, hair, total expressed protein, Mouse, Hairs, house mouse, coat hair, Proteomes, microchaeta, Lanugo389trueThe Proteome of Mouse Vestibular Hair Bundles Over DevelopmentDevelopment of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundles from young (postnatal days P4-P6) and mature (P21-P25) mouse utricles using the twist-off method, then characterized their constituent proteins using liquid-chromatography tandem mass spectrometry with data-dependent acquisition. Using label-free quantitation, we measured relative abundances of proteins in both bundles and in the whole utricular epithelium; comparison of protein abundance between the two fractions allows calculation of enrichment in bundles. These data will be useful for examining the proteins present in mammalian vestibular bundles and how they change over development.2015-08-172015-07-21PXD00216744664499175582580388043330295358511145120678110806124619624619728421880903206375811363350906836351524260275838538791187947398007NCBITaxon:12805723071114301555714683514526236491508472746707364113546138858035823290313888691568895399784475187043511232941262469692812397713310343043654813583334607925198183659219813143205965581914936329572637371922226938124781866132838828139558434513586722294914081691804541619341390363453058611204162326442104117061274432108595614963674283166694369455972945281132081003923751463308793694478420896431744712704459741194669400667145458793299352932010198126469041066114575512165994048339251216597216595160621213312831282128033952321832172920435315228112454349825747874980197525555362312951053948824261914548140479NCBITaxon:215755153439899037120596876231935163098005888455818178081736309431796989361604885661409859566416242515059071187021187053204963063863235554320466322261866331299456586068845351216129567171360441001602358700264203694569410167634102107612773512793342814610029357861746331325291118700215764180969974577444915666675476044524676756411163366411363367102398709NCBITaxon:4055932582359092788396526498217498216347256569156934982116544872056971089448663661410NCBITaxon:4932871556891024598087574171000589452680873231156126373006930391141554219325552187268723872487298727123598755529547624223141463055180066556484198822445299767982510216998231174673897641041051155744689444474153NCBITaxon:5055796016776744685960065349118226370824812991145784586326547876569835566369838192011164034624136810893161001428099612NCBITaxon:548681NCBITaxon:582054641430774794640986099586522831966272905876689605960615729513722187594467087562270861711013354833090349741387834306154319474703342499859961588655974649987039202111072878272154078211908570752061894804189853502471587796271902528674636096469644375939146247274612874601134506253452659197221963852638588596NCBITaxon:61575052110517237576131263419610526419407722774694163481123869NCBITaxon:27036125061747679274596153840052573131368626979699034694171344346360347515363296440421018510976063314961612862879669968583558595968912801726071018568729411009118696141633316780781186941436183NCBITaxon:6191118698840231719118691443906NCBITaxon:1029821000712571189694836419080218787870384766455347095145122578656870861822607046183260705260707NCBITaxon:780NCBITaxon:1502451516658457702921158612184520928574707856100761921263854504457383379192875NCBITaxon:10239NCBITaxon:71428291268063130957044830064175158510555241590841228233446900815025754122810430085210977970671356225833136185509924NCBITaxon:5693826883064052779617946995833424090615101515NCBITaxon:631240674482428130767070876138370828167728503515819901195195337024924053608881756261583558333760212671928532177357267119350184645177217743737111049056371215348112153239576769014726647091571256274579031133355085962859634792491308126387121203511082332648373153214693NCBITaxon:96153832331858626079914126220029813144937608333411149703965583332101841385691006581237561275045426662551519788749200375036009412934971530094114903524725175014012573983649908515151438992NCBITaxon:11552374539883747445446415259311792866432359839062887051580119459932716011497861603293338922853422999810894535936457428412754135122511775058NCBITaxon:1040750277916797181673871349158910306502780291595412629158515849327159570210022612876891355889309451464792031242164133280382803519284343985808946210803481793921182590287889295027906901749342725632273218903829286757221247190400727NCBITaxon:5796537457114311233521140206411981087135547714245072295333555714154293157546224213921390114319338293550833292307411292211484896160106772526499975744727580240550717629936658121405357622941281607839576320667240054029654318431832246596205755499229575757592845933079725518113549706671436733115955644421432138184922311240607210969762261111795752981818431921949633347986626528104660104421454747962678253567097477317513565050NCBITaxon:13136076993894246887955201488751979596214114082241408223421972890922853164351867631854315206494089414424232NCBITaxon:1773214092115778929793244704207559101177499067499073129899136645112273231663170187367451011610358991513284262658721036098334139269611307981336795358893164071034015303337330158191306413992530688391033721233681994044586449811535711471612249111313166343053120175549935129938505731766696426884515134584215726787286238621115104160791298315392227468911983511255228562532326604691361676336316897041328881885511676108077232642486181408434996339376520965731116234273068808637721666898626NCBITaxon:2697049339862410312NCBITaxon:138305959997586448643319729724629176343863725673786391233435105231100905141405449317998686554055348654514712454667605681042986357124679151641358884019373345087711982159739757429719133734275779850762554702563181313686571303443623951655865886223864946454634076NCBITaxon:11071111170828835649184588NCBITaxon:59201108743914594326767111167810774084408797789534967944081226900380394351281459536123517861624323039966235612869597854096409795418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