Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K9me3_zebrafinch_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_K9me3_chicken_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K27cont_01_stagetip497.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K27_cont_4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K27_cont_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K27me3_04_stagetip496.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_K4me3_chicken_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K4K9cont_zebrafinch_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K36me3_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K4K9cont_zebrafinch_08.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K36me3_03_stagetip487.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K36me3_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_H3_stagetip482.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K36me3_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K4me3_zebrafinch_06.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K36me3_zebrafinch_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K36_cont_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_K4me3_stagetip476.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K36_cont_4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K9me3_zebrafinch_05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_K9me3_chicken_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K36me3_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K4K9cont_zebrafinch_06.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_K4me3_chicken_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K4K9cont_zebrafinch_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K36cont_01_stagetip489.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K36_cont_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_control_chicken_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_K9me3_stagetip478.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_K4me3_stagetip474.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K36_cont_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K36me3_02_stagetip486.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K36_cont_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K27me3_03_stagetip495.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K4me3_zebrafinch_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K4K9cont_zebrafinch_05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K27_zebrafinch_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K27cont_02_stagetip498.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_H3_stagetip484.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K36_zebrafinch_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K27me3_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K9me3_zebrafinch_06.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K27me3_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_control_chicken_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K27_cont_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K4me3_zebrafinch_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K27me3_zebrafinch_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K36_zebrafinch_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K27me3_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K27cont_03_stagetip499.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K36me3_zebrafinch_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K9me3_zebrafinch_08.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K9me3_zebrafinch_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K36me3_01_stagetip485.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K27me3_zebrafinch_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K36_cont_4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_H3_stagetip481.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K36me3_zebrafinch_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K4me3_zebrafinch_07.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_K4me3_stagetip475.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K27_cont_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K9me3_zebrafinch_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K4K9cont_zebrafinch_07.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K36cont_04_stagetip492.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K27_cont_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K36me3_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K36me3_4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K27_zebrafinch_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K36_cont_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K4me3_zebrafinch_05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_control_chicken_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K36_cont_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_K9me3_stagetip477.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_K4me3_stagetip473.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_K9me3_chicken_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_K9me3_chicken_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K36me3_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K27_cont_4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K27me3_02_stagetip494.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K4K9cont_zebrafinch_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_K4me3_chicken_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K27me3_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K27me3_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K9me3_zebrafinch_07.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K27me3_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K27me3_01_stagetip493.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_control_chicken_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K36cont_03_stagetip491.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_K9me3_stagetip479.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K36me3_04_stagetip488.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K4K9cont_zebrafinch_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K27me3_4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K27_zebrafinch_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150312_QEP2_FBU_NC_K4me3_chicken_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K36_zebrafinch_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K4me3_zebrafinch_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K27cont_04_stagetip500.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K27me3_zebrafinch_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K36me3_4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_H3_stagetip483.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K9me3_zebrafinch_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150224_QEP2_FBU_NC_K9me3_stagetip480.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K36me3_zebrafinch_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150310_QEP2_FBU_AB_K27_cont_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K27_cont_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K27me3_zebrafinch_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150228_QEP2_FBU_NC_K36cont_02_stagetip490.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_AB_K4me3_zebrafinch_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K27_zebrafinch_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150424_QEP2_FBU_NC_K36_zebrafinch_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150427_QEP2_FBU_AB_K27me3_4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/20150428_QEP2_FBU_NC_K4me3_zebrafinch_08.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/txt_gg.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002282/txt_tg.zipprimaryOK200003600f.butter@imb.deF ButterMass SpectrometryShotgun proteomicsNot availableLc-msmsInteractomeBirdsHistonehttp://www.ebi.ac.uk/pride/archive/projects/PXD002282Cell CultureProteins were eluted in 1x LDS sample buffer supplemented with 0.1 M DTT, boiled for 10 minutes at 70°C and separated on a 4-12% NuPAGE Novex Bis-Tris precast gel (Life Technologies) for 10 min at 180 V in 1xMOPS buffer. Gels were cut into one slice per sample and destained with 50% EtOH and 25 mM ammonium bicarbonate (ABC). After dehydration of the gel pieces with 100% acetonitrile (ACN), samples were dried for 5 min in a concentrator (Eppendorf) and afterwards incubated with reduction buffer (10 mM DTT in 50 mM ABC) for 30 min. The reduction buffer was removed, substituted with alkylation buffer (50 mM IAA in 50 mM ABC) and then subjected to 30 min incubation. Gel pieces were completely dehydrated with ACN and covered in trypsin solution (1 μg trypsin in 50 mM ABC per sample). Proteins were digested over night at 37°C. Tryptic peptides were extracted twice by incubation with extraction buffer (3% TFA and 30% ACN) for 15 min and afterwards with 100% ACN. After reduction of the volume of the elution fraction to about 10-20% in a concentrator (Eppendorf), the peptides were passed through a StageTip. StageTips were prepared using two layers of C18 material (Empore) which was activated with methanol, washed with buffer B (80% ACN, 0.1% formic acid) and equilibrated with two washes of buffer A (50 mM ABC, 0.1% formic acid).PrideNot availableiodoacetamide derivatized residuemonohydroxylated residueacetylated residueRaw files were processed with MaxQuant (version 1.5.2.8) and searched against the ENSEMBL annotated protein database of chicken (gallus gallus: Galgal4.79, 16354 entries) or the zebra finch protein database (taeniopygia guttata: taeGut3.2.4.79, 18204 entries) using the Andromeda search engine integrated into MaxQuant. Carbamidomethyl (Cys) was set as fixed modification, while acetyl (N-term protein) and oxidation (Met) were considered as variable modifications. Trypsin (specific) was selected as enzyme specificity with maximal two miscleavages. Standard MaxQuant instrument settings for the orbitrap were applied. Proteins were quantified with at least 2 ratio counts and based on unmodified unique and razor peptides. LFQ quantification using at least 2 LFQ ratio counts (but without fast LFQ) and the match between runs option were activated.ProteomicsFalk ButterQ ExactivePARTIALInstitute of Molecular Biology, Ackermannweg 4, 55128 Mainz, GermanyGallus Gallus (chicken)Taeniopygia Guttata (zebra Finch) (poephila Guttata)26703087 Bluhm A, Casas-Vila N, Scheibe M, Butter F. Reader interactome of epigenetic histone marks in birds. Proteomics. 2015 Dec 25 10.1002/pmic.201500217f.butter@imb-mainz.deBiologicalQuantitative ProteomicsInstitute of Molecular Biology (IMB)GermanyLysine methylation is part of the posttranscriptional histone code employed to recruit modification specific readers to chromatin. Unbiased, quantitative mass spectrometry approaches combined with peptide pull-downs have been used to study histone methylation-dependent binders in mammalian cells. Here, we extend the study to birds by investigating the interaction partners for H3K4me3, H3K9me3, H3K27me3 and H3K36me3 in chicken (Gallus gallus) and zebra finch (Taeniopygia guttata) using label-free quantitative proteomics. In general, we find very strong overlap in interaction partners for the trimethyl marks in birds compared to mammals, underscoring the known conserved function of these modifications. In agreement with their epigenetic role, we find binding of PHF2 and members of the TFIID, SAGA, SET1 and NURF complex to the activation mark H3K4me3. Our data furthermore supports the existence of a LID complex in vertebrates recruited to the H3K4me3 mark. The repressive marks are bound by the HP1 proteins and the EED subunit of the PRC2 complex as well as by WIZ. Like reported in the previous mammalian screens, we found ZNF462, ZNF828 and POGZ enriched at H3K9me3. However, we noted some unexpected differences. N-PAC (also known as GLYR1), an H3K36me3 interactor in mammals, is reproducible not enriched at this modification in our screen in birds. This initial finding suggests that despite strong conservation of the histone tail sequence, a few species-specific differences in epigenetic readers may have evolved between birds and mammals. All MS data have been deposited in the ProteomeXchange with identifier PXD002282 (http://proteomecentral.proteomexchange.org/dataset/PXD002282).Reader interactome of epigenetic histone marks in birds.Bluhm Alina A, Casas-Vila Nuria N, Scheibe Marion M, Butter Falk FWater, sodium salt, ammonium formate, 13C-labeled, MeCN, cadmium salt, NCMe, zinc formate, lead salt, 2-(indol-3-yl)ethanoic acid, magnesium formate, 1-hydroxyethane, zinc salt, alcohol, Alkylations, peptide, Cut, Polypeptides, Ethanol, ammonium tetraformate, IAA, peptido, BANF, l(1)7Ba, cobalt(II) formate dihydrate, l(1)7Bb, CH3-C#N, Alkohol, B1, Min, DmelCG42273, wood alcohol, ABC, Divorced, C79325, peptides, entire life cycle, 2610036I19Rik, 2610510L13Rik, min, mAPC, proteins, Divorces, ABC14, Aethanol, copper, [CH2Me(OH)], CG11387, AI047805, Dehydrated ethanol, fSAP152, lithium salt, dehydrated, 3-propanediol, ACN, sample, Acn, D1, CT, MCOPCB7, Sodium Methoxide, MTABC3, ammonium (2:1) salt, nickel salt, spiritus vini, Water Stress, Alcohol, cobalt (+2) salt, PRP, Ct, Dmel_CG7826, Ethyl alcohol, (Indol-3-yl)acetate, sodium (4:1:1) salt, Dm1, magnesium salt, IES, BcDNA:GH10590, ETHANOL, nickel (+2) salt, Carbinol, [OEtH], Dmel_CG7835, carbinol, Mnb, MNB, ammonium salt, EtOH, life, formate, cobaltous formate, cromium (+3), AW124434, beta Trypsin, alcohol etilico, Methylcarbinol, METHANOL, Indole-3-acetic acid, Wood, heteroauxin, spirit of wood, copper salt, ammonium (4:1) salt, 2-(bis(2-hydroxyethyl)amino)-2(hydroxymethyl)-1, Polypeptide, methyl cyanide, lead (+2) salt, Methyl Alcohol, umat, lifespan, l(1)VE614, nickel formate dihydrate, lead formate, etanol, aluminum salt, cesium salt, Gene, Methyl, CH3OH, ACINUS, Methylalkohol, DUH3, CG7826, Buffer, formic acid, Aethylalkohol, potassium salt, 14C-labeled, Gene Products, CG7835, CG42273, Wood Alcohol, Separated, Acinus, thallium (+1) salt, entire lifespan, ethanol, beta-Trypsin, rubidium salt, Indoleacetic acid, methanol, calcium formate, methanoic acid, kf, Solution, acinusL, C2H5OH, DmelCG11387, (indol-3-yl)acetic acid, acinusS, wood naphtha, peptidos, cromium (+3) salt, LAN, bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, sodium formate, PTHB1, 1H-indol-3-ylacetic acid, DYRK1, 1-(14)C-labeled, nickel formate, 3H-labeled, Proteins, strontium formate, Methyl alcohol, potassium formate, copper (+2) salt, acetonitrile, strontium salt, buffer, rubidium salt., mKIAA0670, Peptide, Methanol, polypeptide, dry, C18, cupric formate, Protein, alcool ethylique, Sodium, Dyrk1, MeOH, aluminum formate, ACETONITRILE, cyanomethane, AU020952, Separation, lithium formate, Separations, CC1, Bis-Tris, 3-Indolylessigsaeure, Tripcellim, ethanenitrile, sample population, chromic formate, ME-IV, Protein Gene Products, incised, Gene Proteins, Trypure, hydroxyethane, wood spirit, Stress, calcium salt, Peptid, SCH, MethoxideBird, Epigenetics, Epigenetic, avian., Aves, birds, Epigenomiczebra finch, Bru, IPP2A2, 2-amino-4-(methylsulfanyl)butanoic acid, Raw, Hgfr, instrument configuration, STK10, 5730555F13Rik, Gene, protein, protein-containing complex, PHAPII, 5730420M11Rik, peptide, Polypeptides, protein polypeptide chains, HGF, Methionine, peptido, polypeptide chain, template-activating factor I, HGFR, chickens, Gene Products, F15E12.6, Del(8)44H, RCCP2, protein aggregate, Chicken, Fast., Svc, Search Engines, SET, F15E12_6, proportion, peptides, MUB3_18, TAF-I, scatter factor receptor, M, phosphatase 2A inhibitor I2PP2A, ipp2a2, beta-Trypsin, 2pp2a, proteins, MUB3.18, Phasianus gallus, CG10574, AUTS9, DmelCG4299, set, IGAAD, 2PP2A, 2-amino-4-(methylthio)butanoic acid, DmelCG10574, taf-ibeta, ATP:Fas-activated serine/threonine protein phosphotransferase activity, dSET, dSet, peptidos, Poephila guttata, AI838057, RG7MT1, metionina, Racemethionine, FAST, ratio, phapii, proto-oncogene c-Met, DL-Methionine, protein complex, PRO, Gallus gallus domesticus, Proteins, igaad, 2-Amino-4-(methylthio)butyric acid, StF-IT-1, FASTK, Search, 9130215G10Rik, CYS, Peptide, Engine, Gallus gallus, Taenopygia guttata, polypeptide, native protein, natural protein, I-2PP2A, Protein, Dm I-2, I2PP2A, Methionin, methionine, Hmet, and GLY protein 2, Data Base, ATCMPG1, MET, Met, ATCMPG2, HGF receptor, HGF/SF receptor, chicken, and GLY protein 1, Col4a-1, HLA-DR-associated protein II, DI-2, instrument, I-2Dm, tyrosine-protein kinase Met, proportionality, Tripcellim, rate, CG4299, inhibitor of granzyme A-activated DNase, hCMT1c, c-Met, beta Trypsin, I-2PP1, Protein Gene Products, Trypure, Gene Proteins, dSET/TAF-Ibeta, 2610030F17Rik, TAF-IBETA, Gallus domesticus, alpha-amino-gamma-methylmercaptobutyric acid, quotient, Peptid, bantam, TAF-Ibeta, Polypeptide, Par4, variable, AA407739, i2pp2a, 5730455C01Rik, SF receptortype 1, Taf[[II]]150, Set1, Hp1, l(3)72Dj, SET1, Lysine Hydrochloride, alpha 1, Su(Var)2-5, l(2)esg, DmelCG8201, taf40/e(y)1, peroxisomal alanine-glyoxylate aminotransferase deficiency, E(y)1, DmelCG4079, RBP, dmTAF[[II]]230, TFIID 22, Polypeptides, Lysine Acetate, HP1, hp1, HP2, type I, 2, EWSR1, Analysis, TFIID 28, serine pyruvate aminotransferase deficiency, L Lysine, CG3758, Zfp828, strong, DmelCG9874, dgl, TFIID TAF250, Analyses, SETDOMAIN 1, K, cel, TAFII16, nageoire caudale, proteins, Taf[[II]]80, TFIID 28 beta, CG3606, dTAFII85, dTAFii80, Epigenomic, TFIID 42, flg, tail, LYS, Lysin, Role Concepts, TFIID 110, s, SY3-2 TAF110, TAF[[II]]30alpha/28/22, E(var)29A, Bip2, BIP2, Pen19, CG17358, INCURVATA 1, DmelCG6474, Znf828, Su(var), TAF30a, close to, PHF2, Phf2, TAF60/62, Suvar(2)5, dTAF[[II]]110, dTAF[[II]]230, i31, Taf30alpha, TAF30b, TafII24, HP-2, mKIAA1802, HP-1, DmelCG8409, TAF80, Modifications, dTBP, dTbp, ZNF280E, TAF200, dMARK, Taf[[II]]60, Su(var)[205], TAF30alpha/28/22, dTAF[[II]]24, Spectrum Analysis, CG11960, TAFII60, CG30131, CG30132, EMK, AU044539, DmelCG3069, TAFII62, Role Concept, GR1, Histone Modifications, CDYL1, Taf150, DPAR-1, taf110, Role, TAF6L, glycolic aciduria, TAF[[II]]16, dTAFII30 alpha, dTAF[[II]]16, Su-var(2)5, BcDNA:RE73934, activation, Defensin, dmTAF10, Taf6L, Bird, ms(3)570, CHAMP, TAF[[II]]24, l(3)76BDf, Spectrometry, dmTaf10, Taf[[II]]40, hPHF1, alpha, dmTAF10b, Su(var)2-501, dmTAF12, 2610029O15Rik, JHDM1E, TAFII40, DmelCG3169, serine:pyruvate aminotransferase deficiency, Histone Marks, Taf250, BcDNA:RH48823, H3, TAFII40(42), TafII16, dtbp, Mammalia, DEF1, l(1)16Fg, TAFII30alpha/22, Epigenetics, d30beta, Taf85, Taf80, TAF110, TAF230, GLYOXYLATE REDUCTASE 1, pds, dTAF[[II]]155, GTF2D, DmelCG17358, dTAF[[II]]150, Peptidomics, taf24, DmHP1, number, Par-1c, Extra Sex Combs|Enhancer of Zeste complex, HP1B, HP1A, ZNF635m, MTF2L2, TAF[[II]]42, CG7704, TAF[[II]]40, DmelCG5444, dTAF[[II]]262, tough, dTAF150, Gene Products, Set1A, ICU1, Lysine, Gtf2d, TAF[II]80, TAFII60(62), PH1, TBP38, SU(VAR)205, e(y)[1], KMT2F, BM045, 27C1, hyperoxaluria, dTAF[[II]]250, T2DA_DROME, dTAF6L, cell, dTAF[[II]]30beta, ligand, bip-II, CG6711, Su(var)29A, Esg, TAF[[II]]60, SPT3, Hp1a, CENP-35, Hp1b, AA675043, Phasianus gallus, TAF[[II]]62, CG32211, TAF[[II]]30alpha/28 TAF[[II]]22, dTAF250, TAFII80, SETDOMAIN GROUP 1, PAR-1, Prodos, TAFII85, HEL25, SCA17, DEFA2, hp1a, dHP1, GTF2D1, Par-1, peptidos, dTAF62, species, HP1a, Methylations, CG2009, e(y)4, Taf[[II]]110, Mammal, Su[var]205, Proteins, dmTAF6b, dHP-1a, lusk, dTAF10b, TAF40/42/e(y)1, i163, CG5444, BG:DS00004.13, function, TAF[[II]]80, BcDNA:GM09207, Histone, TAF[[II]]85, Cell, dTAF230, Concept, 2810419J22Rik, near to, Taenopygia guttata, Npac, polypeptide, H3K4me3, TAF40/42, Acetate, DmelCG7704, F26B6_3, vertebrates, TAF[[II]]250/230, WAIT1, ZNF635, L-Lysine, d28/22, HP1-VS, Mass Spectrum Analyses, TAF30alpha/28, alanine-glyoxylate aminotransferase deficiency, CURLY LEAF, Taf[[II]]250, chicken, TFIID-p28, DmelCG2009, primary hyperoxaluria type 1, TAF30alpha, 1700029M19Rik, primary structure of sequence macromolecule, AGXT primary hyperoxaluria, dTAF[[II]]30alpha, d40, Gene Proteins, DmelCG6711, TAF30beta, vicinity of, DPar1, bantam, quantitative, dTAF[[40]], Enisyl, l(7)5Rn, TFIID-p42, zebra finch, dTAFII30beta, Par1c, Taf60-2, Taf[[II]]30alpha, CG3069, N-PAC, dTAF12, CG10390, dTAF10, PCCP1, Su(var)2-05, dtafII24, d60, l(2)4B7, peptide, l7Rn5, Mia, ClvPrd, peptido, CG6474, Roles, Code, Taf-6, Concepts, DmTAF3, primary hyperoxaluria type I, Chicken, C13orf8, Chromatins, Histone Mark, dTAF[[II]]30alpha/22, CG8409, Mass Spectrum Analysis, TAF[[II]]150, FBgn0003607, dSpt3, tail fin, peptides, TAF[[II]]155, DmelCG32211, CG3169, hepatic AGT deficiency, epsilon-diaminocaproic acid, ZNF828, uroptère, free, GRC5, d80, F26B6.3, TAFII150, C75991, dme-TAFII40, TAFII155, chromosome scaffold, P19, Gm20194, CG16701, TAF[II]30beta, TAF[[II]]80/85, HEED, nuclear chromatin, E(y)1/dTAF9, glyoxylate reductase 1, taf60, CG8201, DmelCG3758, l(2)07082, Taf[[II]]30beta, anon-Pen19, Gallus gallus domesticus, ZNF803, CG4079, TAFII-250, p19, TAF250/230, uroptérygie, CAMP, Gallus gallus, Spectroscopy, ATGHBDH, TAFII250, dTAFII3, Neutrophil defensin 2, pro, lysine, p22, dTAF42, TAF60-2, TAF[II]40, overlap, TAF[[II]]30beta, p28, dTAF II 150, AI325931, HP1alpha, PCL1, D17Ertd455e, Pcl1, TAF[II]30alpha, su(var)205, AI116001, peroxisomal alanine glyoxylate aminotransferase deficiency, TafII150, Mark, Epigenetic, HP 1-56, Tails, shof, l35Ce, CG17603, TAF[[II]], TAF[[II]]60/62, Taf30beta, br43, l(2)27C1, TAFII110, Gallus domesticus, SR3-5, dTFIID, SR3-3, MARK, TAF[[II]]60-2, p42, DmelCG3606, dtafII16, Polypeptide, dTAF22, CG9874, Su[Var]205, dTAF28, Tctex3, SDG1, DmHP-1, dTAF[[II]]60, TDRD19C, TAF10b, d110, 9530006B08Rik, cytoplasmic chromatin, d230, TAF42, Extra Sex Combs/Enhancer of Zeste complex, TAF40, kmt2, Gene, dTAFII250, TAF[II]150, Spectrum Analyses, EfW1, presence, YTX1, dTAF[[II]]62, dTAFII24, DmelCG10390, dmTAF9, dmTAF8, dmTaf9, dmTAF3, resilient, dmTaf5, AU015913, dmTAF1, Taf110, Taf230, dPAR-1, CG7041, p62, dmTAF6, dTAF[[II]]60-2, dmTAF5, Epigenomic., HL-VIII, DmelCG2859, dmTAF4, Mass, chickens, mammals, HNP-2, HNP-1, SARFH, Taf62, dPar-1, Sarfh, TAF[[II]]30alpha, Mass Spectroscopy, Taf60, TAF250, primary, study, primary hyperoxaluria caused by mutation in AGXT, Taf200, peroxisomal alanine:glyoxylate aminotransferase deficiency, HDL4, PRC2 complex, 3930401K13Rik, Marks, birds, TAF60, l(2)k06323, MRS, CG7128, Taf1p, Taf32, nucleosome remodeling factor complex, pen19, dTAFII150, dTAF[[II]]42, dTAF[[II]]40, prod, CBX5, 4B7, p85, dBIP2, TAF80/85, TAF155, Poephila guttata, AW557215, TAF, TAF150, TFIIDtau, Taf40, l(2)br43, DmelCG7041, SR3-4B, Taf42, dTAF[[II]]30, E(var)29, D8Ertd457e, TFIID-p28beta, AW545332, par1, data, TAF[[II]]250, taf6, avian, TAF[II]110, dPAR1, Taf16, p110, dPar1, l(3)84Ab, dTAF4, dTAFII62, CG2859, Peptide, TfIID, dme-TAFII60, D8Ertd569e, TBP, count in organism, TFIID 62, Zfp462, p230, dTAFII30 beta, anon-WO0210402.19, Protein, HIBDL, sequence, TFIID, Taf28, Vertebrata, Oxalosis 1, Taf22, methylation, Taf24, Vertebrate, Caz, TAF[[II]]40/42, Hp1alpha, Mass Spectrum, dTAF[[II]]80, TAF[[II]]110, TAF[[II]]230, Taf9, TAF9, caudal subdivision, GHBDH, 4930453I21Rik, ENSMUSG00000039373, BG:DS07851.7, cas, CG9348, TAF[II]250, Su(var)2-5, dTAFII110, NP60, dTAFII42, Aves, SUHW5, Protein Gene Products, TAF12, DmelCG17603, TAF10, DmelCG7128, TAF11, TFIID 85, dTAF5, dTAF6, wiz, approaches, dTAF9, Peptid, l(2)35Ce, Suvar2-5, PAR1, Par1, TAF8, 6-diaminohexanoic acid, dTAFII30, TAF6, TAF5, TAF4, TAF3, TAF2, presence or absence in organism, TAF1type 1, Taf[[II]]150, Set1, Hp1, l(3)72Dj, SET1, Lysine Hydrochloride, alpha 1, Su(Var)2-5, l(2)esg, DmelCG8201, taf40/e(y)1, peroxisomal alanine-glyoxylate aminotransferase deficiency, E(y)1, DmelCG4079, RBP, dmTAF[[II]]230, TFIID 22, Polypeptides, Lysine Acetate, HP1, hp1, HP2, type I, 2, EWSR1, Analysis, TFIID 28, serine pyruvate aminotransferase deficiency, L Lysine, CG3758, Zfp828, strong, DmelCG9874, dgl, TFIID TAF250, Analyses, SETDOMAIN 1, K, cel, TAFII16, nageoire caudale, proteins, Taf[[II]]80, TFIID 28 beta, CG3606, dTAFII85, dTAFii80, Epigenomic, TFIID 42, flg, tail, LYS, Lysin, Role Concepts, TFIID 110, SY3-2 TAF110, TAF[[II]]30alpha/28/22, E(var)29A, Bip2, BIP2, Pen19, CG17358, INCURVATA 1, DmelCG6474, Znf828, Su(var), NK/GPI, TAF30a, Data Set., close to, PHF2, Phf2, Gpi, TAF60/62, Suvar(2)5, dTAF[[II]]110, dTAF[[II]]230, i31, Taf30alpha, TAF30b, TafII24, HP-2, mKIAA1802, HP-1, DmelCG8409, TAF80, Modifications, dTBP, dTbp, ZNF280E, TAF200, dMARK, Taf[[II]]60, Su(var)[205], TAF30alpha/28/22, dTAF[[II]]24, Spectrum Analysis, CG11960, TAFII60, CG30131, CG30132, EMK, AU044539, DmelCG3069, TAFII62, Role Concept, GR1, Histone Modifications, Taf150, DPAR-1, taf110, Role, TAF6L, glycolic aciduria, TAF[[II]]16, dTAFII30 alpha, dTAF[[II]]16, NK|GPI, Su-var(2)5, BcDNA:RE73934, activation, Defensin, dmTAF10, Taf6L, Bird, ms(3)570, CHAMP, TAF[[II]]24, l(3)76BDf, Spectrometry, dmTaf10, Taf[[II]]40, hPHF1, alpha, dmTAF10b, Su(var)2-501, dmTAF12, 2610029O15Rik, JHDM1E, TAFII40, DmelCG3169, serine:pyruvate aminotransferase deficiency, Histone Marks, Taf250, BcDNA:RH48823, H3, TAFII40(42), TafII16, dtbp, Mammalia, DEF1, l(1)16Fg, TAFII30alpha/22, Epigenetics, d30beta, Taf85, Taf80, TAF110, Bglap-rs1, TAF230, GLYOXYLATE REDUCTASE 1, pds, dTAF[[II]]155, GTF2D, DmelCG17358, dTAF[[II]]150, Peptidomics, taf24, DmHP1, number, Par-1c, Extra Sex Combs|Enhancer of Zeste complex, HP1B, HP1A, ZNF635m, MTF2L2, TAF[[II]]42, CG7704, TAF[[II]]40, DmelCG5444, dTAF[[II]]262, tough, dTAF150, Gene Products, Set1A, Pgi, ICU1, Lysine, Gtf2d, TAF[II]80, TAFII60(62), PH1, TBP38, SU(VAR)205, e(y)[1], KMT2F, BM045, 27C1, hyperoxaluria, dTAF[[II]]250, T2DA_DROME, dTAF6L, cell, dTAF[[II]]30beta, Gpi-1r, Gpi-1s, ligand, Phi, bip-II, Gpi-1t, CG6711, Su(var)29A, Esg, TAF[[II]]60, SPT3, Hp1a, CENP-35, Hp1b, AA675043, Phasianus gallus, TAF[[II]]62, CG32211, TAF[[II]]30alpha/28 TAF[[II]]22, dTAF250, TAFII80, SETDOMAIN GROUP 1, PAR-1, Prodos, TAFII85, HEL25, SCA17, DEFA2, hp1a, dHP1, GTF2D1, Par-1, peptidos, dTAF62, species, HP1a, Methylations, CG2009, e(y)4, Taf[[II]]110, Mammal, Su[var]205, Proteins, dmTAF6b, dHP-1a, lusk, dTAF10b, TAF40/42/e(y)1, i163, CG5444, BG:DS00004.13, function, MF, TAF[[II]]80, BcDNA:GM09207, Amf, Histone, TAF[[II]]85, Cell, dTAF230, Concept, 2810419J22Rik, near to, Taenopygia guttata, Npac, polypeptide, H3K4me3, TAF40/42, Acetate, DmelCG7704, F26B6_3, vertebrates, TAF[[II]]250/230, WAIT1, ZNF635, L-Lysine, d28/22, HP1-VS, Mass Spectrum Analyses, TAF30alpha/28, NK, alanine-glyoxylate aminotransferase deficiency, CURLY LEAF, Taf[[II]]250, chicken, TFIID-p28, DmelCG2009, primary hyperoxaluria type 1, TAF30alpha, primary structure of sequence macromolecule, AGXT primary hyperoxaluria, dTAF[[II]]30alpha, d40, Gene Proteins, DmelCG6711, TAF30beta, vicinity of, DPar1, bantam, quantitative, dTAF[[40]], Enisyl, l(7)5Rn, TFIID-p42, zebra finch, dTAFII30beta, Par1c, Taf60-2, Taf[[II]]30alpha, CG3069, N-PAC, dTAF12, CG10390, dTAF10, Su(var)2-05, dtafII24, d60, l(2)4B7, peptide, l7Rn5, Mia, ClvPrd, peptido, CG6474, Roles, Code, Taf-6, Concepts, DmTAF3, primary hyperoxaluria type I, Gpi-1, Chicken, C13orf8, Chromatins, Histone Mark, dTAF[[II]]30alpha/22, CG8409, Mass Spectrum Analysis, TAF[[II]]150, FBgn0003607, dSpt3, tail fin, peptides, TAF[[II]]155, DmelCG32211, Nlk, CG3169, hepatic AGT deficiency, epsilon-diaminocaproic acid, ZNF828, uroptère, free, GRC5, d80, F26B6.3, TAFII150, C75991, dme-TAFII40, TAFII155, chromosome scaffold, P19, CG16701, TAF[II]30beta, TAF[[II]]80/85, HEED, nuclear chromatin, E(y)1/dTAF9, glyoxylate reductase 1, taf60, CG8201, DmelCG3758, l(2)07082, Taf[[II]]30beta, anon-Pen19, Gallus gallus domesticus, ZNF803, CG4079, TAFII-250, p19, TAF250/230, uroptérygie, CAMP, Gallus gallus, Spectroscopy, ATGHBDH, TAFII250, dTAFII3, mOC-X, Neutrophil defensin 2, pro, lysine, p22, dTAF42, TAF60-2, TAF[II]40, overlap, TAF[[II]]30beta, p28, dTAF II 150, HP1alpha, PCL1, D17Ertd455e, Pcl1, TAF[II]30alpha, su(var)205, AI116001, peroxisomal alanine glyoxylate aminotransferase deficiency, ORG, Org, TafII150, Mark, Epigenetic, HP 1-56, Tails, Gpi1-r, Gpi1-s, shof, l35Ce, CG17603, Gpi1-t, TAF[[II]], TAF[[II]]60/62, Taf30beta, br43, l(2)27C1, TAFII110, Gallus domesticus, SR3-5, dTFIID, SR3-3, MARK, TAF[[II]]60-2, p42, DmelCG3606, dtafII16, Polypeptide, dTAF22, CG9874, Su[Var]205, dTAF28, Tctex3, SDG1, DmHP-1, Gpi1s, dTAF[[II]]60, TDRD19C, TAF10b, d110, 9530006B08Rik, cytoplasmic chromatin, d230, TAF42, Extra Sex Combs/Enhancer of Zeste complex, TAF40, AI461847, kmt2, Gene, dTAFII250, TAF[II]150, Spectrum Analyses, EfW1, presence, YTX1, dTAF[[II]]62, dTAFII24, DmelCG10390, dmTAF9, dmTAF8, dmTaf9, dmTAF3, resilient, dmTaf5, AU015913, dmTAF1, Taf110, Taf230, dPAR-1, CG7041, p62, dmTAF6, dTAF[[II]]60-2, dmTAF5, HL-VIII, DmelCG2859, dmTAF4, Mass, chickens, mammals, HNP-2, HNP-1, SARFH, Taf62, dPar-1, Sarfh, TAF[[II]]30alpha, Mass Spectroscopy, Taf60, TAF250, primary, study, primary hyperoxaluria caused by mutation in AGXT, Taf200, peroxisomal alanine:glyoxylate aminotransferase deficiency, HDL4, PRC2 complex, 3930401K13Rik, Marks, birds, TAF60, l(2)k06323, MRS, CG7128, Taf1p, Taf32, nucleosome remodeling factor complex, pen19, dTAFII150, dTAF[[II]]42, dTAF[[II]]40, prod, CBX5, 4B7, p85, dBIP2, TAF80/85, TAF155, Poephila guttata, AW557215, TAF, TAF150, TFIIDtau, Taf40, l(2)br43, DmelCG7041, SR3-4B, Taf42, dTAF[[II]]30, E(var)29, D8Ertd457e, TFIID-p28beta, AW545332, par1, data, TAF[[II]]250, taf6, avian, TAF[II]110, dPAR1, Taf16, p110, dPar1, l(3)84Ab, dTAF4, dTAFII62, CG2859, Peptide, TfIID, dme-TAFII60, D8Ertd569e, TBP, count in organism, TFIID 62, Zfp462, p230, dTAFII30 beta, anon-WO0210402.19, Protein, HIBDL, sequence, TFIID, Taf28, Vertebrata, Oxalosis 1, Taf22, methylation, Taf24, Vertebrate, Caz, TAF[[II]]40/42, Hp1alpha, Mass Spectrum, dTAF[[II]]80, TAF[[II]]110, TAF[[II]]230, Taf9, TAF9, caudal subdivision, GHBDH, ENSMUSG00000039373, BG:DS07851.7, cas, CG9348, TAF[II]250, Su(var)2-5, dTAFII110, NP60, dTAFII42, Aves, SUHW5, Protein Gene Products, TAF12, DmelCG17603, TAF10, DmelCG7128, TAF11, TFIID 85, dTAF5, dTAF6, wiz, approaches, dTAF9, Peptid, l(2)35Ce, Suvar2-5, PAR1, Par1, TAF8, 6-diaminohexanoic acid, dTAFII30, TAF6, TAF5, TAF4, TAF3, TAF2, presence or absence in organism, TAF1Bird, Epigenetics, Epigenetic, avian., Aves, birds, Epigenomic360trueReader interactome of epigenetic histone marks in birdsLysine methylation is part of the posttranscriptional histone code employed to recruit modification specific readers to chromatin. Unbiased, quantitative mass spectrometry approaches combined with peptide pull-downs have been used to study histone methylation-dependent binders in mammalian cells. Here, we extend the study to birds by investigating the interaction partners for H3K4me3, H3K9me3, H3K27me3 and H3K36me3 in chicken (gallus gallus) and zebra finch (taeniopygia guttata) using label free quantitative proteomics. In general, we find very strong overlap in interaction partners for the trimethyl marks in birds compared to mammals, underscoring the known conserved function of these modifications. In agreement with their epigenetic role, we find binding of PHF2 and members of the TFIID, SAGA, SET1 and NURF complex to the activation mark H3K4me3. Our data furthermore supports the existence of a LID complex in vertebrates recruited to the H3K4me3. The repressive marks are bound by the HP1 proteins and the EED subunit of the PRC2 complex as well as by WIZ. Like the screens in mammals, we found ZNF462, ZNF828 and POGZ enriched at H3K9me3. However, we noted some unexpected differences. First, we did not observe the enrichment of CDYL and CDYL2 at the repressive marks. Second N-PAC (also known as GLYR1), an H3K36me3 interactor in mammals, is not binding to this modification in our screen. This suggests that despite strong conservation of the histone tail sequence, species-specific differences in epigenetic readers may have evolved.2016-01-042015-06-02PXD0022824466449917558258038804333029535851114510806124619624619728421880903206375811363350906836351524260275838538791187947398007NCBITaxon:128057230711143015557146835145262364927467073641135461388580358233888691568895399784475187043511232946928123977133103430436548135833925198183659219813143205965581914936329572637371922226938124781866132838828139558434513586722294914081691804541619341390363453058611204162326442104117061274432108595614963674283166694369455972945281132081003923751463308793694478420896431744712704459741194669400667145458793299352932010198126469041066114575512165994048339251216597216595160621213312831282128033952321832172920435315228112454349825747874980197525555362312951053948824261914548140479NCBITaxon:21575515343989903712059687623193516309800588845581817808173630943179698936160488566140985956641624251505907118702118705320496306386323555432046632226186633129945658606884535121612956717136044100160235870026420369456941016763410210761277351279334281461002935786174633132529111870021576418096997457744491566667547604452467675663366411363367102398709NCBITaxon:4055932582359092788396526498217498216347256569156934982116544872056971089448663661410NCBITaxon:4932871556891024510005894526808732311561263730391141554219325552187268723872487298727123598755529547624223141463055180066556484198822299767982510216998231174673897641155744689444474153NCBITaxon:5055796016776744685960065349118226370824812991145784586326547876569835566369838192011164034624136810893161001428099612NCBITaxon:548681NCBITaxon:58205464143077479464098609958652283196627766896059606157295137221875944670875622708617110133548330903497413878343061543194747033424996158865597464998703920211107287827215407821190857075206189480418985350247158779627190252867463609646375939146247274612874601134506253452659197221963852638588596NCBITaxon:61575052110517237263419610526419407722774694163481123869NCBITaxon:2703612747679274596153840052573131368626979699034694171344346360347515363296440421097606331492862879669968583558595968912801726071018568729411009118696141633316780781186941436183NCBITaxon:6191118698840231719118691443906NCBITaxon:102982100071257118969419080218787870384766455347095145122578656870861822607046183260705260707NCBITaxon:780NCBITaxon:1502451516658457702921158612184520928574707861921263854504457383379192875NCBITaxon:10239NCBITaxon:71428291268063130957044830064110555241590841228233446900815025754122810430085210977970671356225833136185509924NCBITaxon:569382688306405277965833424090615101515NCBITaxon:63124067448242813076707087613837082816772850351581990119519533702492405360888175626153337602126719285321773572671193501846451772177437371137121534811215323957676901472664709157125627457903113335508596285963479249130821203511082332648373153214693NCBITaxon:961531858614126220029849376083334111497083332101841385691006581237561275045426662551519788749200375036009412934971530094114903524725175014012573983649908515151438992NCBITaxon:11552374539883747445446415259311792866432359839062887051580119459932716011497861603293338922853422999810894535936457428135122511775058NCBITaxon:1040716797181673871349158910306502780291595412629158515849327159570210022612876891355889309451464792031242164133280382803519284343985808946210803481793921182590287889295027906901749342725632273218903829286757221247190400727NCBITaxon:579653745711431123352114020641198108713554771424507229533355571415429315754622421392139011431933829355083329230741129221148489616010677252649997574472758024055071762993665812140535762294128160783957632066724005402965431843183224659620575549922957575759284593307972113549706671436733115955644421432138184922311240607210969762261111795752981818431923347986626528104660104421454747962678253567097477317513565050NCBITaxon:131360769938942468879552014887519795962141421972890922853164351867631854315206494089414424232NCBITaxon:177321409211577892979324470420755910117749906749907312989913112273170187367451011610358132842626587210360983341392696113079813367953588931640710340153033373301581913064139925306883910337212336819940445861153571147161224911131316634305312017554993512993850573176669642688451513458421572678728623862111510416079129831539222746891198351125522856253232136167633631689704132888188551167610807723264248618140843439376520965731116234273068808637721666898626NCBITaxon:2697049339862410312NCBITaxon:1389975864486433197291763438637256737863912334351052311009051414054493179986865540553486545147124546676056810429863571246791884019373345087711982159739757429719133734275779850762554702563188657623951655865886223864946454634076NCBITaxon:1107128835649184588NCBITaxon:592011087439145943267671111678107740844087977895349679440812269003803943512814595361235178616243230399668695978540964097954186942003089544243277869762791292499545115574840905180426430409286924093200302284812313625426428150475102993886538626290341554051208979662877323966920297936289955793157137117008930621864419548663436575584628238235217362315577223994636010621386388420436010439947932184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