Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_3_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_1.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_3_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_2_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_2_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_2_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_2_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_1.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_1.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_1.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_3.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_3.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_2_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_3.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_2.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_1_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_1_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_1_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_3.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_3.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_3.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_1_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_1_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_2_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_1_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_1_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_1_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_3.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_3_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_2.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_3_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_3_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_3_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_2_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_2.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_2.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_2_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_1.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_2.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_3.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_3_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_1.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_2.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_1.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_2.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_1.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_2.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_3_ProteinPilot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_3.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_3.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_3.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_1.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_3.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_3.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_1.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_2.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_3.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_3.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_1.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_2.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_3.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_3.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_1.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_1.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_3.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_2.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_1.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_3.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_2.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_2.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_2.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_3.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_3.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_1.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_1.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_2.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_2.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_3.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_3.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_3.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_decoy.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_3.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_1.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_1.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_2.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_1.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_3.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_2.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_3.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_1.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_1.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_1.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_3.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_2.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_2.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_1.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_1.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_2.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_2.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_3.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_3.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_2.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_3.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_3.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_2.dat-pride.pride.mztab.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_2.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_1.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_1_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_1_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_3.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_3_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_1.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_3.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_3_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_1.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_2_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_3.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_3.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_1_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_2_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_3.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_2.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/NC_000962.faaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_1.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_2.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_3_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_2_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_2.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_2_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_1_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_1_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_1.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp45_1.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_1_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_1.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_3.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_3.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp7_3_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_3.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp1_3_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_3_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_3_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_2_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_1_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_1.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_1_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_2.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp13_2_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_3_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp27_2.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp10_2_decoy.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_2.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/Sp22_2.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002542/H37Rv_2_decoy.datprimaryOK200103280ilinaen@gmail.comIlya AltukhovMass SpectrometryShotgun proteomicsMatrix Science Mascot 2.2.07BeijingTuberculosisLc-ms/msMtbhttp://www.ebi.ac.uk/pride/archive/projects/PXD002542Protein Extraction from M. tuberculosis. The bacterial cells pellet were suspended in 50 μL lysozyme and 100 μL 100 mМ TrisHСl pH 7.6 with 3 µL of Protease inhibitor Mix (GE Healthcare, USA). Cells disrupted on a bead-beating homogenizer (MPBio, FastPrep-24, USA) with 0.5-mm silica-zirconium beads, time at 4 min followed by 5 min on ice. To reduce disulfide bonds and to block cysteine residues the collected suspension was added by sodium-dodecyl-sulfate (SDS) (Panreac, Spain) to a final concentration of 10 % and dithiotreitol (DTT) (BioRad, USA) to a final concentration of 10 mM and incubated at 60 °C for 30 min. The sample was by centrifugation at 13,000 g, 4 °С for 5 min. Protein concentration was measured by the Bradford method using the Bradford Protein Assay Kit (BioRad, USA). Trypsin Digestion. Two hundred micrograms of protein was loaded onto a 7.5% SDS-PAGE gel and separated on a PROTEAN II (Bio-Rad, USA) at 20 mA for 20 min and 40 mA overnight. The gel was stained with Colloidal blue. Proteins were excised in 1x1 mm pieces that were destained in 10 mM DTT in 100 mM ammonium bicarbonate for 30 min at 56oC, followed by a second wash in 50 mM ammonium bicarbonate in 50% (v/v) acetonitrile (ACN) (1-2 h, 50oC). Proteolytic digestion was performed with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega, USA) in a 1:50 enzyme:protein (v/v) ratio at 37 °C overnight (approx 16 h). The cleavage was stopped by adding 5% formic acid (FA) and peptides were extracted in a solution containing 50% ACN and 5% FA (2v/v) followed by extraction in 75% ACN and 5% FA (2v/v). Peptides were concentrated by speedvac and dissolved in 20 μL 1% acetic acid. The supernatant peptides were removed and cleaned using C18 Sep-Pak columns (Waters, USA). LC-MS/MS analysis. Analysis was performed on a TripleTOF 5600+ mass-spectrometer with a NanoSpray III ion source (ABSciex, Canada) coupled to a NanoLC Ultra 2D+ nano-HPLC system (Eksigent, Singapore). The HPLC system was configured in a trap-elute mode. For a sample loading buffer and buffer A, the mix of 98.9% water, 1% methanol, 0.1% formic acid (v/v) was used. Buffer B was 99.9% acetonitrile, 0.1% formic acid (v/v). Samples were loaded on a trap column Chrom XP C18 3 mm 120 Å 350 mm*0.5 mm (Eksigent, Singapore) at a flow rate of 3 ul/min over 10 min and eluted through the separation column 3C18-CL-120 (3 mm 120 Å) 75 mm*150 mm (Eksigent, Singapore) at a flow rate of 300 nl/min. The gradient was from 5 to 40% of buffer B in 120 min. The column and the pre-column were regenerated between runs by washing with 95% of buffer B for 7 min and equilibrated with 5% of buffer B for 25 min. Between the samples to ensure the absence of carryover both the column and the precolumn were thoroughly washed with a blank trap-elute gradient that included five 7 minute 5-95-95-5% B waves followed by 25 min 5% B equilibration. Mass spectra were acquired in a positive ion mode. Information-dependent mass-spectrometer experiment included 1 survey MS1 scan followed by 50 dependent MS2 scans. MS1 acquisition parameters were as follows: mass range for analysis and subsequent ion selection for MS2 analysis was 300-1250 m/z, signal accumulation time was 250 ms. Ions for MS2 analysis were selected on the basis of intensity with the threshold of 200 cps and the charge state from 2 to 5. MS2 acquisition parameters were as follows: resolution of quadrupole was set to UNIT (0.7 Da), measurement mass range was 200-1800 m/z, optimization of ion beam focus was to obtain maximal sensitivity, signal accumulation time was 50 ms for each parent ion. Collision activated dissociation was performed with nitrogen gas with collision energy ramping from 25 to 55 V within 50 ms signal accumulation time. Analyzed parent ions were sent to dynamic exclusion list for 15 sec in order to get the next MS2 spectra of the same compound around its chromatographic peak apex (minimum peak width throughout the gradient was about 30 s).PrideNot availableS-methyl-L-methionineS-carboxamidoethyl-L-cysteinePeptide identification. Raw data (.wiff files) were converted to Mascot Generic Format (.mgf files, peak lists) using command-line program AB SCIEX MS Data Converter v.1.3 (AB SCIEX, Framingham, MA, USA) with “-proteinpilot” parameter. Mascot v. 2.2.07 was used for the identification against Mycobacterium tuberculosis H37Rv sequence database (3932 amino acid sequences, including 26 contaminants sequences) downloaded from RefSeq database [Tatusova T, Ciufo S, Fedorov B, O'Neill K, Tolstoy I. RefSeq microbial genomes database: new representation and annotation strategy. Nucleic Acids Res. 2014 Jan 1;42(1):D553-9] (RefSeq: NC_000962.3). The Mascot searches were conducted with the following parameters: tryptic digestion, the peptide mass tolerance of 10 ppm, fragment mass tolerance of 0.5 Da, variable modifications caused by Oxidation (M) and Propionamide (C), maximum 1 missed cleavages, a peptide charge state limited to 1+,2+ and 3+.ProteomicsElena IlinaTripleTOF 5600MD, PhD, DSc Head of laboratory of Molecular microbial genetics Research Institute of Physical-Chemical ( lab head )COMPLETEMycobacterium Tuberculosis Sp1Mycobacterium Tuberculosis Sp13Mycobacterium TuberculosisMycobacterium Tuberculosis Sp7Mycobacterium Tuberculosis Sp22Mycobacterium Tuberculosis Sp10Mycobacterium Tuberculosis H37rvilya.altukhov@gmail.com27356881 Bespyatykh J, Shitikov E, Butenko I, Altukhov I, Alexeev D, Mokrousov I, Dogonadze M, Zhuravlev V, Yablonsky P, Ilina E, Govorun V. Proteome analysis of the Mycobacterium tuberculosis Beijing B0/W148 cluster. Sci Rep. 2016 Jun 30;6:28985 10.1038/srep28985BiologicalBiomedicalResearch Institute of Physical Chemical Medicine10.6019/PXD002542Beijing B0/W148, a "successful" clone of Mycobacterium tuberculosis, is widespread in the Russian Federation and some countries of the former Soviet Union. Here, we used label-free gel-LC-MS/MS shotgun proteomics to discover features of Beijing B0/W148 strains that could explain their success. Qualitative and quantitative proteome analyses of Beijing B0/W148 strains allowed us to identify 1,868 proteins, including 266 that were differentially abundant compared with the control strain H37Rv. To predict the biological effects of the observed differences in protein abundances, we performed Gene Ontology analysis together with analysis of protein-DNA interactions using a gene regulatory network. Our results demonstrate that Beijing B0/W148 strains have increased levels of enzymes responsible for long-chain fatty acid biosynthesis, along with a coincident decrease in the abundance of proteins responsible for their degradation. Together with high levels of HsaA (Rv3570c) protein, involved in steroid degradation, these findings provide a possible explanation for the increased transmissibility of Beijing B0/W148 strains and their survival in host macrophages. Among other, we confirmed a very low level of the SseA (Rv3283) protein in Beijing B0/W148 characteristic for all «modern» Beijing strains, which could lead to increased DNA oxidative damage, accumulation of mutations, and potentially facilitate the development of drug resistance.Proteome analysis of the Mycobacterium tuberculosis Beijing B0/W148 cluster.Bespyatykh Julia J, Shitikov Egor E, Butenko Ivan I, Altukhov Ilya I, Alexeev Dmitry D, Mokrousov Igor I, Dogonadze Marine M, Zhuravlev Viacheslav V, Yablonsky Peter P, Ilina Elena E, Govorun Vadim V328liquid chromatography tandem mass spectroscopy, multicellular organismal catabolic process, single-organism catabolic process, host organism, Gene Ontology Projects, DNS, Mycobacterium tuberculosis variant tuberculosis, single-organism developmental process, determination, (Deoxyribonucleotide)n, Peptidomics, Monocyte Derived Macrophages, Biocatalysts, vif Gene, drug susceptibility/resistance, number, vif, Gene, protein, A Genes, sor Gene, protein-containing complex, steroid catabolism, presence, supernumerary, Gene Ontology Project, Gene Ontology, Deoxyribonucleic acids, LC-MS-MS, MAGE-E1 antigen, Mutations, protein polypeptide chains, Drug resistance., polypeptide chain, Deoxyribonucleic Acid, Mycobacterium tuberculosis var. hominis, LC-MSMS, Monocyte-Derived Macrophage, Gene Products, symptoms, Project, Low, protein aggregate, Sprains, drug resistance, Bacillus tuberculosis, A, Bacterium tuberculosis, increased, thymus nucleic acid, me75, LCMSMS, reference sample, enzymes, catabolism, sor Genes, Q, Double Stranded, long-chain fatty acid biosynthesis, proteins, Deoxyribonucleic acid, Bone Marrow-Derived Macrophage, AI847422, free, ecotype, D17Mit170, T1, Bone Marrow-Derived, Enzyme, Peking, HCA1, time of survival, long-chain fatty acid anabolism, Double-Stranded DNA, Strains, (Deoxyribonucleotide)m, deoxyribonucleic acids, DNAn, A Gene, long-chain fatty acid formation, Controlled, LC-MS2, screening, Controlling, findings, cou, degradation, steroid degradation, protein complex, DNAn+1, Sprain, Proteins, Alpha-dystrobrevin-associated MAGE Protein, LC-MS/MS, total expressed protein, long-chain fatty acid synthesis, enzyme activity, sor, Monocyte Derived, Double-Stranded, Tl3, Tl2, results, strain, (Deoxyribonucleotide)n+m, development, count in organism, LC/MS/MS, Monocyte-Derived, Lr, survival, native protein, natural protein, Protein, chemical analysis, DAMAGE, Hepatocellular carcinoma-associated protein 1, Strain, Resistance, Ontology Projects, cultivar, ds-DNA, desoxyribose nucleic acid, Q Gene, Monocyte-Derived Macrophages, Macrophage, Ontology Project, Gene Ontologies, Ontology, vif Genes, Q Genes, Genes, breakdown, Ontologies, Projects, RGD1560259, Macrophages, increased number, Mycobacterium tuberculosis H37Rv, response to drug, signs, pooled, Bone Marrow Derived Macrophages, Protein Gene Products, Drug, Gene Proteins, present in greater numbers in organism, liquid chromatography-tandem mass spectroscopy, ds DNA, steroid breakdown, Biocatalyst, Bone Marrow-Derived Macrophages, Desoxyribonukleinsaeure, Bra, liquid chromatography tandem mass spectrometry, assay, quantitative, DNA, Strains and Sprains, mMage-e1, Proteomes, accessory, Mycobacterium tuberculosis typus humanus, presence or absence in organismBru, Clo, Raw, AW549739, Slf, SLF, Aminosaeure, Mycobacterium tuberculosis str. H37Rv, Somatomedin-C, Aminocarbonsaeure, Amino acid, FBN, FPH2, alpha-amino acid, contrasted, peptide, Polypeptides, acides nucleiques, Drug Tolerance, ClvPrd, peptido, ECTOL1, Del(8)44H, WMS, C79691, Svc, amino acids, peptides, Genomes, somatomedin-C, SF, Kitl, Mast cell growth factor, Lccp, OCTD, mKIAA0989, Con, acidos nucleicos, 10^[-6], IGF1, ppm, Mechano growth factor, blz, peptidos, Soluble KIT ligand, Sl, GPHYSD2, drug tolerance, SGS, IGF-I, data, steel factor, acide nucleique, immune system tolerance, hematopoietic growth factor KL, Aminokarbonsaeure, somatomedin, alpha-amino acids, IBP1, Mycobacterium sp. H37Rv, Peptide, alpha-amino carboxylic acids, sKITLG, Programs, ACMICD, polypeptide, Immune Tolerance, nucleic acids, Nucleic Acid, mechano growth factor, sequence, Immunologic Tolerance, NA, MFS1, Igf-1, Data Base, WMS2, Amino Acid, Acid, SHEP7, Mycobacterium tuberculosis strain H37Rv, Col4a-1, Nukleinsaeure, mast cell growth factor, Amino acids, Mycobacterium tuberculosis H37Rv, Stem cell factor, MASCOT, acido nucleico, MASS, STAT5, Amino, primary structure of sequence macromolecule, Nucleic, stem cell factor, Kitlg, KL-1, Self Tolerance, Tolerance, MGF, Mgf, Nukleinsaeuren, c-Kit ligand, SSKS, KITLG, Peptid, Acids, z., Polypeptide, SCF, variable, Gb, Immunological ToleranceKoch's Disease, Koch Disease, Peking., determination, Infections, Kochs Disease, chemical analysis, TB, Infection, Kochs disease, total expressed protein, Tuberculosis, Mycobacterium tuberculosis, assay, Mycobacterium tuberculosis Infection, Tuberculoses, Mycobacterium tuberculosis Infections, Proteomes, active tuberculosis, tuberculosis diseaseBacillus tuberculosis, total expressed protein, Bacterium tuberculosis, Peking., assay, Mycobacterium tuberculosis variant tuberculosis, determination, Proteomes, Mycobacterium tuberculosis var. hominis, chemical analysis, Mycobacterium tuberculosis H37Rv, Mycobacterium tuberculosis typus humanussodium salt, 4-Dithiothreitol, CPD photolyase activity, ammonium formate, 13C-labeled, IL1BC, MeCN, cadmium salt, PLXN5, PhrB photolyase activity, ion, Vinegar, CASP-1, rad, SDH, magnesium formate, fs(1)M34, zinc salt, Long Term, RP11-508D10.1, SeP, 5730420M11Rik, dmTAF[[II]]230, SDS, Polypeptides, protein polypeptide chains, KL receptor activity, M(3L)i, E260, CEH, cobalt(II) formate dihydrate, Chloride(1-), CH3-C#N, B1, SCO5, SEC, 1, NEPII, CG4601, 3, SCO1, NUP96, germacrene A synthase activity, Analysis, Sodium Ion, SEH, outer pigmented layer of retina, Il1bc, Gsfsow3, epithelium, WMS, Sep, SEP, (R*, SET, Divorced, type 5 acid phosphatase, C79325, TFIID TAF250, Analyses, DHO, cel, DmelCG13176, M, N, phosphatase 2A inhibitor I2PP2A, sec, CG18572, ORF19, proteins, Divorces, W, 4-dithiothreitol, T6G21.3, Acetic Acid Glacial, IL-1BC, HPLC, sEP, SUPPRESSOR OF AUXIN RESISTANCE 3, AI047805, DmelCG4299, allergic reaction, PA1, set, T-cell antigen Gp39, pigment epithelium of retina, column, ACN, sample, Acn, D1, MeCO2H, n, pigmented retina epithelium, Bs, ACT, s, TNFSF5, D9, Half Cystine, M(3)RpS17, z, Inorganic, nickel salt, SGS, LC-MS2, sulfate, dTAF[[II]]230, Sulfate dianion, Stars, Caspase-1 subunit p10, Zinc Cysteinate, cobalt (+2) salt, Tnfrsf5, Cr, TAF200, Longterm Effect, GAST, Spectrum Analysis, not genetically inherited, tuberculosis disease, DmelCG7788, ACMICD, hCD40L, Inorganic Sulfates, PBT, chrome, sodium (4:1:1) salt, Glacial, Dm1, magnesium salt, Stickstoff, M(3)i(55), Su(b), tetraoxosulfate(2-), DmelCG42628, sodium, IMD3, dipyrimidine photolyase (photosensitive), Raps, 1110049F14Rik, 6-trans-farnesyl-diphosphate diphosphate-lyase (germacrene-A-forming) activity, natrium, HAP1, Hydrogen Oxide, rpS17, pbt, reticulate acropigmentation of Dohi, Cl-, HLA-DR-associated protein II, Ethylic acid, Chrom, DI-2, Sulfates, I-2Dm, proportionality, formate, NKTL, dyschromatosis symmetrica hereditaria 1, Spectrometry, Dmel_CG15720, pooled, rate, cromium (+3), inhibitor of granzyme A-activated DNase, beta Trypsin, breadth., I-2PP1, APE1, nitrogen, TAF-IBETA, Taf250, liquid chromatography-tandem mass spectroscopy, spirit of wood, Drice, ammonium (4:1) salt, TAF-Ibeta, methyl cyanide, Mycobacterium tuberculosis Infection, DRICE, lead (+2) salt, STARS, 4-Dimercapto-2, Methyl Alcohol, TAF230, krk1, l(1)Ab, F10B6_15, rac-Dithiothreitol, APEN, nickel formate dihydrate, lead formate, Effects, threo-1, Ly62, DrIce, DrICE, Cleland's reagent, TAPK, apex, aluminum salt, dyschromatosis symmetrica hereditaria, 2-trans, CH3OH, precursor, protein-containing complex, thomson, TR-AP, body system, SO4(2-), DmelCG5692, CG7826, F10B6.15, deoxyribodipyrimidine photolyase activity, stratum pigmentosum retinae, mass-to-charge ratio, Ion Level, period, M(3)i, sensitive, LC-MSMS, GP39, CG7835, Gene Products, CG42273, system, tetraoxosulfate(VI), Half-Cystine, APEX, gp39, CD154, T6K12_14, proportion, anatomical systems, dTAF[[II]]250, FAM39E, GKLP, CATC4, pins, Acinus, Longterm, thallium (+1) salt, cell, L Cysteine, absent from organism, PCE-2, beta-Trypsin, rubidium salt, F23A5.3, 4733401P19Rik, 2pp2a, uL/min, Long-Term, methanoic acid, l(3)dtOA4, ions, PINS, CG10574, Apex, Solution, CD156, acinusL, fs(1)829, dTAF250, PCTAIRE2-binding protein, MGC:45012, 2PP2A, Caspase-1 subunit p20, acinusS, DmelCG18572, M(3)i[55], dSET, dSet, peptidos, Mycobacterium tuberculosis, Cl(-), PTHB1, 3-butanediol, proto-oncogene c-Kit, NTKL, AcOH, CPS, CG5692, 1-(14)C-labeled, Kochs Disease, caspase 3, Proteins, Mell1, Methyl alcohol, BG:DS00004.13, potassium formate, CE-2, DSH, DSH1, copper (+2) salt, ACP5, chloride, acetonitrile, SPENCDI, buffer, (+)-(10R)-germacrene A synthase activity, ribosome-associated ubiquitin-dependent protein breakdown, Cell, Dithiothreitol, mKIAA0670, dTAF230, Sulfate(2-), polypeptide, Wash2, Wash1, Experiment, native protein, I-2PP2A, KIT ligand receptor activity, A-7, C18, cupric formate, Long Term Effects, chemical analysis, Dm I-2, TAF[[II]]250/230, rel-(2R, MFS1, BcDNA:RE44119, MIXL, Sodium, MeOH, aluminum formate, Pins, Mass Spectrum Analyses, DTL, XKrk1, Sodium Ion Level, T6K12.14, Taf[[II]]250, AU020952, hypersensitivity, DTT, lithium formate, RPE, PYR1, Separations, M(3)q, Striated muscle activator of Rho-dependent signaling, Na, Chloride, Natrium, SWDS, IL-1 beta-converting enzyme, ethanenitrile, azote, Interleukin-1 beta-converting enzyme, CD117, CD156a, retinal pigment epithelium, anon-EST:Posey48, Longterm Effects, ME-IV, p. pigmentosa retinae, plan specification, Gene Proteins, Ref-1, 4732433M03Rik, C-Kit, MeCOOH, ACETIC ACID, Ssm, xkl-1, CG4346, quotient, M(3L)i[55], liquid chromatography tandem mass spectrometry, Chlorine anion, DRORUD, deoxyribonucleate pyrimidine dimer lyase (photosensitive), REF-1, TRACP, APE, Sodium 23, Sulfate, NEP2, Ethanoic acid, Methoxide, sulphate ion, liquid chromatography tandem mass spectroscopy, D1Ucla3, Rps17, IPP2A2, NCMe, APX, determination, selection process, cleavage, zinc formate, Nl1, RPS17, CG7788, crice, lead salt, Essigsaeure, protein, Xkl-1, CG15720, pigmented epithelium, csp2, peptide, M(3)67, Tnfsf5, sodio, Gsfsco1, ammonium tetraformate, peptido, BANF, Glacial Acetic Acid, cromo, NL1, Tuberculosis, Min, NL2, protein aggregate, REF1, Effect, Gsfsco5, chloride(1-), MIX, DmelCG42273, SOW3, wood alcohol, Koch's Disease, Mass Spectrum Analysis, T-BAM, Koch Disease, rsh, 24Cr, LCMSMS, tartrate-resistant acid ATPase, [SO4](2-), peptides, TAF-I, SULFATE ION, 2610036I19Rik, l(3)67BDo, 2610510L13Rik, E430039A18Rik, Acetic Acid, pigmented retina, min, mAPC, copper, 3R)-1, microlitres per minute, SCAN, DNA cyclobutane dipyrimidine photolyase activity, TRAP3, IGAAD, fSAP152, lithium salt, Tudor repeat associator with PCTAIRE-2, SOLO DANCERS, DmelCG10574, ICE, TB, C16orf53, ethoic acid, Sodium Methoxide, T5ap, Sl, GPHYSD2, MGC - 45012, Long-Term Effects, ammonium (2:1) salt, Dmel_CG4346, ratio, symmetric dyschromatosis of the extremities, CD40L, phapii, (+)-germacrene A synthase activity, PRE, Alcohol, ice, iCE, drice, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, LC-MS/MS, Cd40l, Dmel_CG7826, M(3)67C, AI836084, StF-IT-1, Tr-kit, Th, 4-dimercapto-2, 4-disulfanylbutane-2, TAFII-250, pigmented retinal epithelium, TAF250/230, Spectroscopy, Sodium-23, TAFII250, batch, retinal pigment, Ionen, MMEL2, drIce, drICE, kl1-A, TNF-related activation protein, Sulfate anion(2-), KIT, WASH, GAS, nickel (+2) salt, Ly-62, Carbinol, retinal pigment layer, GAT, Dmel_CG7835, Acetic acid, carbinol, Mnb, MNB, parent ion, tyrosine-protein kinase Kit, MILD1, ammonium salt, absence, MS/MS, ribosome-associated degradation, retinal pigmented epithelium, P45, Rp S17, familial reticulate acropigmentation of Dohi, cobaltous formate, AW743063, ribosome-associated ubiquitin-dependent protein degradation, L-Cysteine, kit, MASS, CG4299, gas, AW124434, AI326936, CG17603, TAF[[II]], REM3, organ system, R*)-1, METHANOL, nanospray, Wood, phr A photolyase activity, DNA-photoreactivating enzyme, precursor ion, SR3-5, TrATPase, copper salt, IGM, hypersensitivity reaction disease, p45, CD40LG, Eph2, Polypeptide, 7N, RAD1, i2pp2a, time, active tuberculosis, CHLORIDE ION, d230, E-260, Cysteine Hydrochloride, Balearic Islands, TRAP, p50, cesium salt, SCF receptor activity, rapsyn, FBN, Gene, CH3-COOH, dTAFII250, PLEXIN-B1, Methyl, antibiotic A 7, ACINUS, Spectrum Analyses, photoreactivating enzyme activity, Methylalkohol, EfW1, PHAPII, LC-MS-MS, formic acid, Buffer, method, Methanecarboxylic acid, potassium salt, M(3)S33, polypeptide chain, scfr, template-activating factor I, dmTAF1, Ms1, Taf230, ECTOL1, p63, 14C-labeled, method used in an experiment, p65, Mass, Kochs disease, Wood Alcohol, stratum pigmentosa retinae, Disulfide, CD40-L, Level, SCFR, Separated, sensitivity, CH3CO2H, Mass Spectroscopy, Pro-Mega, TAF250, Taf200, Fdc, CG2916, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, FOCUS, Infections, PRECOCIOUS, MS1, MS2, iones, Selb, ipp2a2, Minute, Taf1p, methanol, Mycobacterium tuberculosis Infections, calcium formate, OCTD, DmelCG3922, Suspension, TEIF, Trap, wood naphtha, taf-ibeta, male sterility 1, SELP, ribosome-associated ubiquitin-dependent protein catabolism, HIGM1, Long-Term Effect, cromium (+3) salt, hypersensitivity reaction, TAF, sodium formate, F23A5_3, C78062, P105, DYRK1, TAF[[II]]250, CG42628, bead, nickel formate, 3H-labeled, protein complex, PCTAIRE2BP, 6-trans-farnesyl-diphosphate diphosphate-lyase [(+)-germacrene-A-forming] activity, igaad, Canary Islands, strontium formate, l(3)84Ab, sulphate, Bp50, acropigmentation of Dohi, strontium salt, Peptide, Methanol, Dithiotreitol, sep5, stratum pigmentosum (retina), LC/MS/MS, MODIFIER OF SNC1, secret agent, CAD, HOAc, natural protein, p230, Protein, DL-threo-1, Chloride ion, deoxyribonucleic photolyase activity, I2PP2A, Infection, TFIID, Dyrk1, CES2A1, Tuberculoses, connected anatomical system, ACETONITRILE, tandem MS, cyanomethane, WMS2, CG13176, Mass Spectrum, Separation, c-KIT, 10^[-9], hypersensitive, TAF[[II]]230, tetraoxidosulfate(2-), E 260, photolyase activity, MALE STERILITY 1 PROTEIN, 3-diol, CC1, DmelCG2916, Tripcellim, TAF[II]250, acide acetique, CGI-97, Sulfuric acid ion(2-), sample population, chromic formate, CG3922, nitrogeno, Interleukin-1 beta convertase, Protein Gene Products, Trypure, chromium, dSET/TAF-Ibeta, 11Na, c-kit, 2610030F17Rik, DmelCG17603, Ion, concentration, wood spirit, SSKS, 3.4.22.36, calcium salt, Peptid, INS No. 260, RAD, Rad, assay, acetic acid, Chromium, SCH, AA407739, TAF1host organism, lung neoplasms, d230, human being, Luzp5, Infestations and Infections, Gene, dTAFII250, EfW1, dmTAF[[II]]230, Sec-dependent secretion system-associated complex, dmTAF1, parenchyma of lung, Taf230, responsivity, lung, Gene Products, Kochs disease, Tuberculosis, CAPG2, neoplasm, neoplasm of the lung, Sprains, TAF250, Koch's Disease, reactivity, portion of tissue, Koch Disease, tumor of lung, Taf200, dTAF[[II]]250, pattern, TFIID TAF250, Infections, cel, 5830426I05Rik, cell, distribution, Tissue, Taf1p, proteins, Mycobacterium tuberculosis Infections, man, dTAF250, Infestation and Infection, mCAP-G2, tumor of the lung, Clients, TB, main terminal branch, Mycobacterium tuberculosis, Infections and Infestations, Strains, TAF, Mtb, MTB, lung neoplasm (disease), tumour of lung, neoplasm of lung, dTAF[[II]]230, TAF[[II]]250, pulmo, Kochs Disease, lung tumor, Proteins, Sprain, lung parenchyma, TAF200, total expressed protein, l(3)84Ab, BG:DS00004.13, TAFII-250, TAF250/230, Client, Cell, results, tuberculosis disease, dTAF230, T2SS-associated complexes, TAFII250, p230, Protein, Proteomes., proteomic analysis, Strain, Infection, tissue portion, TAF[[II]]250/230, TFIID, simple tissue, Tuberculoses, hCAP-G2, neoplasms, LUZP5, Taf[[II]]250, TAF[[II]]230, CAP-G2, general secretion pathway-associated complex, Infection and Infestation, TAF[II]250, tumour of the lung, CG17603, TAF[[II]], human, Protein Gene Products, Gene Proteins, DmelCG17603, pulmonary, Taf250, Patient, SR3-5, Lungs, lung tumour, response, lung neoplasm, Mycobacterium tuberculosis Infection, Strains and Sprains, active tuberculosis, TAF230, TAF11trueProteome analysis of the M. tuberculosis Beijing B0/W148 clusterThe main project purpose is to investigate the fundamental physiological state of M. tuberculosis (MTB) during the infection and the mycobacterial response within the infected host tissue, human lung. High-throughput proteomic analysis of MTB cells will be involved to solve this problem. The description of pattern of proteins expressed in MTB cells extracted directly from clinical material of patients with tuberculosis defines the main novelty of the given project. Undoubtedly, the intermediate project results describing the features of MTB strains proteome in vitro will also be essential for the global scientific community.2016-07-062015-07-22PXD00254244544100904054998638804333038783884019961575748782721513519850765557148021589103066239803100226190269157463601355881134506146479145943NCBITaxon:6157118259040813803942725631459537227243230746992867NCBITaxon:245306279129249703612518028481235551504751029999034694176945347515597293632997966287157546224295486317447575584628228723074111481736231489693529372512646903994672721659554440426071012803218184318357559312811836575930797219080214321382426191065922261260705260707885318702945586265281736309698936160488209285678251249668619235554NCBITaxon:10359NCBITaxon:1313456586068879557959704483006412354436763107622430816996328104215745771216979423213562233474726970498030102392976010117381899131639101165691569313076749409833440817287613837089901198839370210005899940343055542853217731193501872631714505118723872487279564818822954767091115104580023055148015490313847562360385962982510151098231174673493211676275967767373153NCBITaxon:961529722573493760833341911079276280863632666891031298382375617492003197196627960615729517110125673727356881