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Quantitative profiling of post-translational modifications by immunoaffinity enrichment and LC-MS/MS in cancer serum without immunodepletion. Mol Cell Proteomics. 2015 Dec 3. pii: mcp.O115.052266"],"submitter_affiliation":["Cell Signaling Technology"],"labhead_mail":["hongbo.gu@cellsignal.com"],"technology_type":["Affinity purification coupled with mass spectrometry proteomics","Mass Spectrometry"],"disease":["Acute Leukemia","Breast Cancer","Non-small Cell Lung Carcinoma"],"submitter_keywords":["Ptm","Cancer serum"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD002931"],"quantification_method":[""],"modification":[""],"data_protocol":["Raw data was searched by Sequest and identified peptides were quantified by label-free quantification"],"omics_type":["Proteomics"],"species":["Homo Sapiens (human)"],"submitter_mail":["hongbo.gu@cellsignal.com"],"curator_keywords":["Technical","Biomedical"],"submitter_country":["United States"],"pubmed_abstract":["A robust method was developed and optimized for enrichment and quantitative analysis of posttranslational modifications (PTMs) in serum/plasma samples by combining immunoaffinity purification and LC-MS/MS without depletion of abundant proteins. The method was used to survey serum samples of patients with acute myeloid leukemia (AML), breast cancer (BC), and nonsmall cell lung cancer (NSCLC). Peptides were identified from serum samples containing phosphorylation, acetylation, lysine methylation, and arginine methylation. Of the PTMs identified, lysine acetylation (AcK) and arginine mono-methylation (Rme) were more prevalent than other PTMs. Label-free quantitative analysis of AcK and Rme peptides was performed for sera from AML, BC, and NSCLC patients. Several AcK and Rme sites showed distinct abundance distribution patterns across the three cancer types. The identification and quantification of posttranslationally modified peptides in serum samples reported here can be used for patient profiling and biomarker discovery research."],"pubmed_title":["Quantitative Profiling of Post-translational Modifications by Immunoaffinity Enrichment and LC-MS/MS in Cancer Serum without Immunodepletion."],"pubmed_authors":["Gu Hongbo H, Ren Jian Min JM, Jia Xiaoying X, Levy Tyler T, Rikova Klarisa K, Yang Vicky V, Lee Kimberly A KA, Stokes Matthew P MP, Silva Jeffrey C JC"],"additional_accession":[]},"is_claimable":false,"name":"Serum PTM Identification_Technical Triplicates","description":"A technical triplicate experiment was performed to validate the robustness of the method using pooled serum of non-small cell lung cancer 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