Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/mqpar.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/Animal_20140915_YP_COLLAB_NOA_B-FQ2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/Animal_20140915_YP_COLLAB_NOA_B-FQ1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/Animal_20140915_YP_COLLAB_NOA_B-FQ3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/Animal_20140915_YP_COLLAB_NOA_A-FQ3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/Animal_20140915_YP_COLLAB_NOA_C-FQ1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/Animal_20140915_YP_COLLAB_NOA_A-FQ2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/Animal_20140915_YP_COLLAB_NOA_C-FQ3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/Animal_20140915_YP_COLLAB_NOA_C-FQ2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/Animal_20140915_YP_COLLAB_NOA_A-FQ1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003120/combined.rarprimaryOK200003120geiger@tauex.tau.ac.ilYair PozniakAffinity purification coupled with mass spectrometry proteomicsMass SpectrometryBreast CancerNot availableCox-2Nfkb2HomeostasisCell-migrationTp53Cancerhttp://www.ebi.ac.uk/pride/archive/projects/PXD003120Epithelial CellCell CultureCell pellets were lysed using NP-40 lysis buffer. For MS analysis of p53 immunoprecipitant a mixture of PAb1801, DO-1, PAb421 antibodies was used. Lysates were incubated with the bound antibodies for 16 hours at 4oC and washed with NP-40 lysis buffer.For MS analysis the beads were additionally washed with 150mM NaCl, 50mM Tris pH=8.0 and proteins were released from beads using on-beads trypsin digestion as previously described (Hubner et al. 2010). Peptides were then purified on C18 stageTips.PrideMS1 intensity based label-free quantification methodphosphorylated residueMS raw files were analyzed by MaxQuant (version 1.5.0.25 (Cox and Mann 2008)). MS/MS spectra were searched against the reference Uniprot human proteome by the Andromeda search engine (Cox et al. 2011). False Discovery Rate (FDR) of 0.01 was used on both the peptide and protein levels and determined by a decoy database. Peptides and proteins were quantified using a label-free algorithm in MaxQuant. Prior to bioinformatics analysis, the resulting protein list was filtered to eliminate common contaminants and decoy database hits.ProteomicsMultiomicsTamar GeigerQ ExactiveThe Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, IsraelPARTIALHomo Sapiens (human)26588988 Furth N, Bossel Ben-Moshe N, Pozniak Y, Porat Z, Geiger T, Domany E, Aylon Y, Oren M. Down-regulation of LATS kinases alters p53 to promote cell migration. Genes Dev. 2015 29(22):2325-2330 10.1101/gad.268185.115yair.poz@gmail.comBiomedicalTel Aviv Universityp53 is a pivotal tumor suppressor and a major barrier against cancer. We now report that silencing of the Hippo pathway tumor suppressors LATS1 and LATS2 in nontransformed mammary epithelial cells reduces p53 phosphorylation and increases its association with the p52 NF-κB subunit. Moreover, it partly shifts p53's conformation and transcriptional output toward a state resembling cancer-associated p53 mutants and endows p53 with the ability to promote cell migration. Notably, LATS1 and LATS2 are frequently down-regulated in breast cancer; we propose that such down-regulation might benefit cancer by converting p53 from a tumor suppressor into a tumor facilitator.Down-regulation of LATS kinases alters p53 to promote cell migration.Furth Noa N, Bossel Ben-Moshe Noa N, Pozniak Yair Y, Porat Ziv Z, Geiger Tamar T, Domany Eytan E, Aylon Yael Y, Oren Moshe Mbeta[[3]]-Tub, DmelCG17117, d230, dp53, determination, p50, antigen NY-CO-13, p53, Gene, dTAFII250, 143391_i_at, beta3 TU, lysis, EfW1, betaTub3, LFS1, Tp53, l(3)05745, Buffer, dmTAF[[II]]230, peptide, Polypeptides, halite, DMP53, peptido, dmTAF1, Taf230, bbl, Gene Products, Dmp53, anon-EST:Liang-2.13, tumor suppressor p53, TP53, TAF250, 1323/07, Taf200, dTAF[[II]]250, clone 2.13, TFIID TAF250, peptides, BCC7, cel, cell, D.m.BETA-60D, beta-Tub6D, dmp53, beta-Trypsin, chlorure de sodium, T, Taf1p, DmP53, 1422/04, proteins, cloruro sodico, dTAF250, B3t, salt, DmelCG3401, Trp53, Dp53, D1, betaTub60C, Kochsalz, phosphoprotein p53, beta3-tubulin, peptidos, autolysin activity, necrosis, beta[[3]]-tubulin, TRP53, TAF, CG17117, p50/tubulin, PTHB1, beta-Tub60D, CG10873, dTAF[[II]]230, TAF[[II]]250, rock salt, Dmbeta3, Dm-HTH, BETA 60D, prac, Proteins, beta3t, beta3-Tub, TAF200, l(3)84Ab, BG:DS00004.13, Mischung, TAFII-250, TAF250/230, antibodies, buffer, Cell, Peptide, Xp53, dTAF230, 3t, polypeptide, TAFII250, common salt, Natriumchlorid, C18, p230, p53/tubulin, chemical analysis, Protein, TAF[[II]]250/230, TFIID, bacteriocin activity, table salt, bfy, beta[[3]] tubulin, NaCl, Tub60D, HTH, Hth, Taf[[II]]250, CG33336, beta-tub, TAF[[II]]230, holin, DmelCG33336, D-p53, beta60C, Tripcellim, CG3401, beta3Tub, beta3TUB, Dm-P53, l(3)86Ca, TAF[II]250, beta3, CG17603, natrii chloridum, TAF[[II]], beta Trypsin, dtl, B1., Tub, Protein Gene Products, DTB3, Gene Proteins, Trypure, Meis1, hth1, DmelCG17603, lysin activity, hth2, Taf250, SR3-5, betatub60D, P53, bacteriolytic toxin activity, p44, bhy, Peptid, assay, Polypeptide, CG31325, TAF230, betaTub, TAF1, sodium chloridebeta[[3]]-Tub, DmelCG17117, dp53, Dlats, p50, antigen NY-CO-13, p53, Lats/Warts, 143391_i_at, Wts, beta3 TU, betaTub3, LFS1, Downregulation, Tp53, l(3)05745, DMP53, bbl, l(3)100Aa, Dmp53, anon-EST:Liang-2.13, tumor suppressor p53, TP53, 1323/07, clone 2.13, BCC7, D.m.BETA-60D, beta-Tub6D, dmp53, p50/tubulin., T, CG12072, DmP53, 1422/04, WTS/LATS, DmelCG12072, B3t, DmelCG3401, Trp53, Dp53, betaTub60C, phosphoprotein p53, beta3-tubulin, dmLATS, beta[[3]]-tubulin, TRP53, CG17117, beta-Tub60D, CG10873, lats, Dmbeta3, Dm-HTH, BETA 60D, prac, beta3t, beta3-Tub, MENE (3R)-G, LATS, Down Regulation, Xp53, 3t, Down-Regulation, Receptor Down-Regulation, Down-Regulation (Physiology), p53/tubulin, Warts, bfy, beta[[3]] tubulin, wart, Tub60D, HTH, Hth, CG33336, beta-tub, DmelCG33336, D-p53, beta60C, CG3401, beta3Tub, beta3TUB, Dm-P53, l(3)86Ca, beta3, dtl, Tub, DTB3, Meis1, hth1, hth2, wts/lats, lts, betatub60D, warts/lats, Warts/Lats, P53, Lats, p44, bhy, Receptor, CG31325, Wts/Lats, betaTubprotein levels, Bru, cox4, human being, Raw, determination, False, protein complex, T5E21.12, Proteins, total expressed protein, Gene, Search, protein, Data Base., protein-containing complex, Peptide, Engine, polypeptide, peptide, CG10664, Polypeptides, protein polypeptide chains, ClvPrd, peptido, native protein, natural protein, polypeptide chain, VA, AL024441, IV, Algorithm, Protein, chemical analysis, Gene Products, IV-1, Del(8)44H, COX IV, DmelCG10664, protein aggregate, Data Base, T5E21_12, Svc, Search Engines, proportion, Coproporphyrinogenase, F, Col4a-1, peptides, Va, COXIV, Coprogen oxidase, COX IV-1, tend, proportionality, common, rate, proteins, COXA_DROME, man, free, human, Protein Gene Products, Gene Proteins, CG14724, Cox4, COX, quotient, Peptid, peptidos, 1.3.3.3, DmelCG14724, Polypeptide, assay, COX-VA, Proteomes, Cox4a, ratiomalignant Growth, FKBP52, FKBP59, dp53, Tfb2, TFB2, Epithelial Cell, tumor suppressor, Nbla10317, CDP, Clox, Lymphocyte signal transduction molecule alpha 4, Tumor, Glandular, betaTub3, FKBP51, Tp53, phosphorylation, CDP1, cell cycle regulator, DMP53, CUX, Hsp56, Associations, bbl, anon-WO0118547.648, Alpha4 phosphoprotein, Dmp53, cancer of the breast, Dmp52, tumor suppressor p53, TP53, Cux/CDP, BC, 1323/07, AI662457, LEDGF, AW545633, BCC7, neoplasm (disease), beta-Tub6D, dmp53, T, DmP53, 1422/04, CG7764, Adenomatous Epithelial, CA, CVID10, B3t, DmelCG3401, Dp53, regulation of cell cycle progression, malignant neoplasm, cancer of breast, NF-kappaB2, phosphoprotein p53, associated, CG17117, COY1, p50/tubulin, Transitional Epithelial Cell, CG10873, malignancy, lyt, PPIase, HBI, Adenomatous, Squamous, Transitional Epithelial, LYT10, 4932411G09Rik, 3t, p50B, nc16, arrest of mitotic cell cycle progression, organ system cancer, modulation of cell cycle progression, NOS, bfy, breast carcinoma, Tub60D, HTH, Hth, Adenomatous Epithelial Cell, beta-tub, Adenomatous Epithelial Cells, Tumor., Squamous Cell, DmelCG33336, CG3401, beta3Tub, beta3TUB, C81413, H2TF1, CUTL1, DTB3, hth1, DmelCG7764, hth2, AV277261, breast cancer, CDP/Cut, P52, Cells, P53, regulation of cell cycle arrest, p44, bhy, p49, cancer, carcinoma of the breast, CG31325, cell cycle regulation, betaTub, PAIP, beta[[3]]-Tub, DmelCG17117, p50, GOLIM6, conformation, antigen NY-CO-13, p52, cell type cancer, p49/p100, p53, 143391_i_at, beta3 TU, Glandular Epithelial, LFS1, 154538_at, l(3)05745, CASP, Squamous Epithelial Cells, anon-EST:Liang-2.13, wts, neoplasm, Squamous Epithelial, malignant tumour, Epithelial, clone 2.13, AW208785, NF-kB2, D.m.BETA-60D, p75, mammary carcinoma, Trp53, control of cell cycle progression, regulation of progression through cell cycle, Epithelial Cells, Transitional Epithelial Cells, betaTub60C, p200, ms(3)nc16, beta3-tubulin, beta[[3]]-tubulin, DFS70, TRP53, Squamous Epithelial Cell, relational structural quality, negative regulation of cell cycle arrest, Glandular Epithelial Cell, Transitional, beta-Tub60D, cell cycle modulation, Squamous Cells, Dmbeta3, PSIP2, Dm-HTH, KPM, cell cycle arrest, Tap42, malignant, ASC1p100, carcinoma of breast, BETA 60D, prac, fkbp52, beta3t, beta3-Tub, Phosphorylations, Protein phosphatase 2|4|6 regulatory subunit, p110, fkbp59, Cell, Xp53, positive regulation of cell cycle arrest, MT, Cuboidal Glandular Epithelial Cells, AW208599, p53/tubulin, malignant neoplasm (disease), WARTS, p100, p105, Columnar Glandular Epithelial Cells, TFIIH, BTF2 p52, beta[[3]] tubulin, p49|p100, CG33336, primary cancer, hsp56, cell cycle control, CD79a-binding protein 1, l(3)E35, D-p53, beta60C, Glandular Epithelial Cells, Dm-P53, l(3)86Ca, Pc52, beta3, AI115586, malignant tumor, dtl, warts, mitotic cell cycle arrest, Tub, Meis1, malignant neoplastic disease, betatub60D, AW228608, LYT-10, breastmalignant Growth, FKBP52, FKBP59, dp53, Tfb2, TFB2, Epithelial Cell, tumor suppressor, Nbla10317, CDP, Clox, Lymphocyte signal transduction molecule alpha 4, Tumor, Glandular, betaTub3, FKBP51, Downregulation, Tp53, phosphorylation, CDP1, cell cycle regulator, DMP53, CUX, Hsp56, Associations, bbl, anon-WO0118547.648, Alpha4 phosphoprotein, Dmp53, cancer of the breast, Dmp52, tumor suppressor p53, TP53, Cux/CDP, BC, 1323/07, AI662457, LEDGF, AW545633, BCC7, neoplasm (disease), beta-Tub6D, dmp53, T, DmP53, 1422/04, CG7764, Adenomatous Epithelial, CA, CVID10, B3t, DmelCG3401, Dp53, regulation of cell cycle progression, malignant neoplasm, cancer of breast, NF-kappaB2, phosphoprotein p53, associated, CG17117, COY1, p50/tubulin, Transitional Epithelial Cell, CG10873, malignancy, lyt, PPIase, HBI, Adenomatous, Squamous, Transitional Epithelial, LYT10, 4932411G09Rik, 3t, p50B, nc16, Receptor Down-Regulation, arrest of mitotic cell cycle progression, organ system cancer, modulation of cell cycle progression, NOS, bfy, breast carcinoma, Tub60D, HTH, Hth, Adenomatous Epithelial Cell, beta-tub, Adenomatous Epithelial Cells, Tumor., Squamous Cell, DmelCG33336, CG3401, beta3Tub, beta3TUB, C81413, H2TF1, CUTL1, DTB3, hth1, DmelCG7764, hth2, AV277261, breast cancer, CDP/Cut, P52, Cells, P53, regulation of cell cycle arrest, p44, bhy, p49, cancer, carcinoma of the breast, CG31325, cell cycle regulation, betaTub, PAIP, beta[[3]]-Tub, DmelCG17117, p50, GOLIM6, conformation, antigen NY-CO-13, p52, cell type cancer, p49/p100, p53, 143391_i_at, beta3 TU, Glandular Epithelial, LFS1, 154538_at, l(3)05745, CASP, Squamous Epithelial Cells, anon-EST:Liang-2.13, wts, neoplasm, Squamous Epithelial, malignant tumour, Epithelial, clone 2.13, AW208785, NF-kB2, D.m.BETA-60D, p75, mammary carcinoma, Trp53, control of cell cycle progression, regulation of progression through cell cycle, Epithelial Cells, Transitional Epithelial Cells, betaTub60C, p200, ms(3)nc16, beta3-tubulin, beta[[3]]-tubulin, DFS70, TRP53, Squamous Epithelial Cell, relational structural quality, negative regulation of cell cycle arrest, Glandular Epithelial Cell, Transitional, beta-Tub60D, cell cycle modulation, Squamous Cells, Dmbeta3, PSIP2, Dm-HTH, KPM, cell cycle arrest, Tap42, malignant, ASC1p100, carcinoma of breast, BETA 60D, prac, fkbp52, beta3t, beta3-Tub, Phosphorylations, Protein phosphatase 2|4|6 regulatory subunit, p110, fkbp59, Cell, Down Regulation, Xp53, positive regulation of cell cycle arrest, Down-Regulation, MT, Cuboidal Glandular Epithelial Cells, AW208599, Down-Regulation (Physiology), p53/tubulin, malignant neoplasm (disease), WARTS, p100, p105, Columnar Glandular Epithelial Cells, TFIIH, BTF2 p52, beta[[3]] tubulin, p49|p100, CG33336, primary cancer, hsp56, cell cycle control, CD79a-binding protein 1, l(3)E35, D-p53, beta60C, Glandular Epithelial Cells, Dm-P53, l(3)86Ca, Pc52, beta3, AI115586, malignant tumor, dtl, warts, mitotic cell cycle arrest, Tub, Meis1, malignant neoplastic disease, betatub60D, AW228608, Receptor, LYT-10, breastbeta-Tub60D, beta[[3]]-Tub, CG10873, DmelCG17117, dp53, lats, Dmbeta3, Dm-HTH, Dlats, p50, antigen NY-CO-13, p53, BETA 60D, Lats/Warts, prac, beta3t, beta3-Tub, MENE (3R)-G, 143391_i_at, LATS, Wts, beta3 TU, betaTub3, LFS1, Tp53, l(3)05745, Xp53, 3t, DMP53, p53/tubulin, bbl, Warts, l(3)100Aa, Dmp53, bfy, beta[[3]] tubulin, anon-EST:Liang-2.13, tumor suppressor p53, wart, Tub60D, HTH, Hth, TP53, CG33336, beta-tub, 1323/07, clone 2.13, BCC7, D.m.BETA-60D, beta-Tub6D, dmp53, DmelCG33336, D-p53, beta60C, CG3401, p50/tubulin., beta3Tub, beta3TUB, T, CG12072, DmP53, Dm-P53, l(3)86Ca, 1422/04, beta3, dtl, WTS/LATS, DmelCG12072, Tub, DTB3, B3t, Meis1, DmelCG3401, hth1, Trp53, hth2, wts/lats, lts, Dp53, betatub60D, warts/lats, Warts/Lats, betaTub60C, P53, Lats, phosphoprotein p53, p44, bhy, beta3-tubulin, dmLATS, beta[[3]]-tubulin, TRP53, CG17117, CG31325, Wts/Lats, betaTub3120trueDownregulation of LATS kinases alters p53 to promote cell migrationp53 is a pivotal tumor suppressor and a major barrier against cancer. We now report that silencing of the Hippo pathway tumor suppressors LATS1 and LATS2 in non-transformed mammary epithelial cells reduces p53 phosphorylation and increases its association with the p52 NF-κB subunit. Moreover, it partly shifts p53’s conformation and transcriptional output towards a state resembling cancer-associated p53 mutants, and endow p53 with the ability to promote cell migration. Notably, LATS1 and LATS2 are frequently downregulated in breast cancer; we propose that such downregulation might benefit cancer by converting p53 from a tumor suppressor into a tumor facilitator.2018-10-252015-10-29PXD003120280655164609918049829535851114513335244224942024527872842188090368093270429330845811363336351524260119834300662758385NCBITaxon:12806888856189611143011872635555711452623649346136403195206512695736664923916197467073853883641135461582158231066568895390531136364762721123294422291180992323122586365458333460792519365914320597129386558195851149315242592637371250232NCBITaxon:12721119577481866148858431043002451358672256807617554373592540816918045426683429531945305861266835149822505814963674452158554528113208147956410039237514636942049433208964208963445974137979138712728384400667203275453293529367156759388036478434643867716052165994048321659721659545501315282693042165915627161696833916243218321729204277944762376454317203094980197525556978159509439843948840479NCBITaxon:2157525338551531051067439899037122857759515205968762262311210884555588845587077918178081736309431796758144571698936160488409859NCBITaxon:371621047826794015059071483643204210613204913816936303555432046632633311994565453511025411103217459216129264201288676410026420369456941016763410210761277351524867131658239254727933428146135843014172987609174633457744491675476048932128735675684030135062191726518130194111929439367110411310239320255598632582788396591047594120347495109716049016529983552566366817585439801102432608663102459808757417555183201964471452680457966313670190832331156114155355104219325552113834397634981812359871069955529NCBITaxon:4751850298335476113615741465477180066556484337677144137929976798251021699823897641400820840741400821137351155711089725482444474153NCBITaxon:50557899698446752510003646534933414714749354801946751299114359741276473137086326547876565893639835322159589366983819201110677577271034819852348780265554479464098609958615718717668599191572951372211711012929210768783497419860202809392499148840633424923423515233598598865591324974649987039202111072943311711393635272159874190850371170827117082841823502414511396929319021978224746360461471237472269402375939723287581722532566481219071905079452659600131972211915853101774208588596NCBITaxon:615750521028307989515586411051989972375059576131720002634196102641088878176179212190659407121906772275885815689964975151950582873757244NCBITaxon:22023717NCBITaxon:170361220199985705060506172407476792745062724915961533355433355418400524643213686150596487844612286662941786661347515363291018194301086731018510184NCBITaxon:1763573039635586331492862876705801321650852718481865368595105135553482260710185687294296110091416333NCBITaxon:619184023210007348824123363630710470058127187878645159812894555663577553470951451225786425011449447260704NCBITaxon:13356262607052607076584574054163677483167233150561007133705654195NCBITaxon:305550445791399878168119011928752829126806315327341478281055524NCBITaxon:960613585846900811116781502283051930283672859300852900561742NCBITaxon:569382688306401794699151011849952622543525875913151590144824283811139442258441604193704759272120441437086042328167728503515819901195195326738637013702492405360883858162248839432186156227748817562615835583715333760572671193501998086119350216787910857913711371272407190650371475917262981288057125627457572709030903113335503728142433747924910078711082NCBITaxon:96157243359463318586260799141262200298147802492670NCBITaxon:12637192812492125283038568385695911692661006581373523756127504542666255151978813566383750644223360094129349790546322455525363524721401257515115199764990851515143899237453747445446415243235937605920222887051580595536555088157932716029385157811497863389229382894535936479437509173NCBITaxon:6854412754225117752991590109013416738715895521012966103061296715851584515849376593934327159100226376733889147923590461423451287689591198332966467041464792936576164280341160221641331599280382803536909108917688149894621812141080348179392287889295027113133295282829445686174934681911265269972273218903821076012471904007271597369978281143114020641198108713554774365814245072295337969820423212422312916321803347087145372091645733215358157546266940401379928777020206032811431933829355083329129221148643745725264999728303573908718308726727103790855071762997076789268370415573729214053651388294128160783956392431426265489822737756115596207322086595882735551693605330797255181135491432137159749535026115955614321384060725878141447335215094933491949634016143347280059360463164610466076290338151845474485003567097477265669771951240174841855601335755547920427415805360769946881037620148873297261005941200375828909316435428268314256850071230383990013894649408382835941442423221978542337742171598921409212205853939621157781154756929793244704749906604162103591905158114452749907312987779913112273170187546991103589915NCBITaxon:372961036098334187104911307981336795316407299467103401578658264530333733063830113064139925306423636505911696547885469811211620343176526161033728612994044586207340115357111952965261174553111471613386541313166343055213231201798360253237312761255232993512993850573163176426197940115134584457726787286232193341513461862086211607913922279835168119612552281495393223231167617573121080772134879999488618569628140843410335177879963218496209948873213924882184932097115338209611533920953304393751441467569927612686266447NCBITaxon:2697049339862436941103121958832050920290610130685999758644458828643249952529724642606674115834321271789427863789426147740525673786391233435514199834931799868655405534352914865451471245466889251042986357124679151641358257632884019373345087718238039129039129529719748693179789110286889995133731054878665220341712516321389850762554701193806222523256318118781865713034431121308516558658862238NCBITaxon:446114488964946454636493NCBITaxon:1107114280062421241873032883564913743751415167845881087439145943854331116781077854314408043933452703134NCBITaxon:1108295346232269397738218993182269003512838714595311688533996695271067888695berry954186942003089544869724436620665634701509545300558623355180426430869220030228481242642815047514991052728311029910298471472414452386262903195418329025120858623195574974311700893062186441930615755841310165382352308056557722382359603939946360106601623601043994839947179748286790039651354440423761021279056881337751823635572116229629817677187093128112147861050238245185579669465431863205182406672829744173944932351245012885318388919867948853191113782559103716472332713516601261557228331349390NCBITaxon:101142845031631297801985738595239693934323795602608712134639598427529595266437334323223544314275242243081433619593133456582609432749592560291699631122233605201216979525283869728529046436801218707334747279010132342956031278161119219408188297603818124988563122034018843973312744141639437552107243139855858885872520493692955464803834369122432640817212419355955217493991281614055920425465519458228860918382727592127442340817019332982972275985955712744201143179127442623545555285712072943284139961660187625482964718761437868149024748011991509564818822972543176101214801541204551481672384738481882945190695150303416633603616598404849850569310151052835352835269373529451263753405206698741864972972240521572477258594109767741856451804812310061911079450950NCBITaxon:813478009412694322710152275279434472568484792911463870333849917226175610946161435120325812051365218505235259447320549423349373473864527000651214466449917591891582580388043330376686244804612067811220260499108061246196290222461973206375229918670511694186705750906812193879118794718680239800718680157230721545146835741258140818611757150847691838858032903116086113888691521677811472767212711036839978445972475129513987043512317191262469692812397713333995226126103430481381781821981318826821080228156100547624112292657163295755350192222197911119686669381979121247124614854510407132838828139510802302949110401459541619341390363120416221106658903264460711117022721041170648800312744321085956210283169412831666943694559729949514106588827114113161879010105841330879478447517415080817053942065421055687317447127070996415329242814132630119466984114545879327695379329201019812646904106615505371457551392519232392320140815764013169731606212133928303123839112831282242605128033952281101083282525353152851281128533531534982574194814787536231424101356742951056500242619145481NCBInr 201305191150621294597064731292829963394134695975386312117051288193516265390021343980313098006978103690122020718853225660173654856615664162425103695118702348334824688221187056386321219690226186129925016468606885656512623477702172195658101028656061656064428386567171360416023587003494150480221601002988235786132529111870014477162157641809699756655666452467134887937965223926291613633661383366336721751117645126793406560103789059232146429314344870933114767468342808NCBITaxon:40559767465235909322646523652621263374982178707498216175831634725656905691569256938722498211654487205697345076111327999300166690510894491089448NCBITaxon:2759661410NCBITaxon:4932653587155689520710894441000589655087324470942875351352481263730069303926659581143988725121177687268723872487291178665498727134976724223110677410677330554417725784541581419198822445446NCBITaxon:120661174673104105909882497473446899601677674468587529600NCBITaxon:581111822637082484119165578457845848402119443955307158059656636164034624136810893161001428092644629612NCBITaxon:548681NCBITaxon:582054641439450330775531995797552283637379196627290581526009605960687594467087562270862665964960432325957050933548330902982938783481062675672675664306151279365431947448385449239470472112298411915231917016108961548313969169287827573674544078211293145707529835206189257771285024803308352701262834854189854871427867158779627300251563041126736125994952869646964396444417684911462472746128746011345069638526385263996563024097639245264110909467469416348112386935174635231NCBITaxon:565995576784218192119562330591736582200879325731352167426979699034613443463605132964404213451124951533043479096109760616194032257329246200966927294227294351338968583554767894314696891280175576009681118696932011167807811869414361833303811869817194759321718118691443906NCBITaxon:10298NCBITaxon:7771257118387271566338404330618596948364190802703847664568703257310568708618218678661833568851350NCBITaxon:7803906451515NCBITaxon:150299031645151694312970298207621158612184512107257309193079496866109446091374944002092852013197470781384040529507619212638546146663915383379NCBITaxon:10239NCBITaxon:714173613095705470448300641751585923890037159084128108406972282334575412NCBITaxon:1024528104NCBITaxon:10243392017481097797067748111356225833136185509924286706527796583342409062004929102634949539321034809NCBITaxon:6312406741307670703766191009408707487613810804148942329032176023993592672947474376032768917812126719285321773846451772177437272557104905615348119922512153239576769014NCBITaxon:722715697472664709131704725852092026971303276978596285963411476130913081307126387121203533264837315321469338323411479398158370131413134937608333413118333357047111497039655397283332101841833312146874550451080187492002627241530091076620637373505411490280148517501303713444143983NCBITaxon:115523988139927515622435623593117928662623168390614404211945995677716032932853422NCBITaxon:5759345089998103365786185336583517431275307796457425457428316273121536024271831627713528086913519948875058NCBITaxon:104073162755678024271550277916797182079541349660027130627415496753524502780291595412644323929158660025570212721135588675512349161296587930945203124135810515192843435815398580414717414718156269811825902471491331678456099069027256027256122142725633544228410133562634166392867572279017904NCBITaxon:57965374575740574113467611233523562378548355522221045775126216131091575596915429310136413962242139213901857323271489622392307411640278128810489616010675744580240251229683352944139366581403661208660576257634432206672400540296543NCBITaxon:11709140827428231772981843183443226224697657554992295757793657592845935561822845907066714367334442403531849223112105421152763910969762261111795752981882633184319229438152764098666134312416265281044214438679626782531751327804267608565050176946996NCBITaxon:131317125079533894279558801198732519795962141140822414082232645614219710141228518676318543152076773767774219253066129454458057041744726724042649329188912NCBITaxon:17732088932088952075591011766455315231663302004367451011613284266567226587213926963588954230195318829388399158196655665788391943665341123368131359044982249115505543826413103379986669400688456668421576711435353115104298315468911129859356253232273075660469136165105612149165238976336316812149159704126308213494091328881885513613NCBITaxon:1865381889083264241916073NCBITaxon:1865366210173660411856503157503937651851651573111623427306855743680863772163157491529526689535862048867NCBITaxon:138862962305959755284003197291761608994291703002731052311009110090405410092646532243232760568340621786061064539477816198215973975749980241994742757758042277793623719552491313662392410734073130014640766253388812041044156845111170869374515479221198561198572992014625944072NCBITaxon:5920165636713926767162481450134408440877111745479778745461074016269941279131478196794967954081408238039452813534038165966887229261235178616243231243230135992356127108767175895549785409640979788210352930524327762791292491155748409097804092409344713512025316376162983863317810081631362515200223886585694415540181339717416870979662877323966920297936289931563430192234471376343387222795486634362991662823345643316917362312592485861109366742138638842049321135472484794731447311696811129025516139812848131733367221440772131693155577812211122152250255183610036NCBITaxon:112661860078NCBITaxon:43948811313231867944848514549414645637499010041715916922596345910659213334991065901249615334542NCBITaxon:662243275442712432745365521340112390110049716571678496410289688333295491249668425282652158109434326521577160197127908571622562237NCBITaxon:103572702NCBITaxon:1035811948816543605076011415784435590NCBITaxon:1035917072127263480081859347834132234380221134458262762618903641969471395019825286349559839642711913645272621272623272624116071911771891238993121410246052312189484032118213142697049403248031131808030490314179813052124834311147787490932384814371131536889187891841368417494080498040578822593194342714918283734256136491154373413156713164163159403021009714629772823525675060139172618951929524929145051115249649222748492496731460519518766260201153348580021538186256226912328013603275614231422275119030414340119110221450520493210175527591428493426641799069141746091110205117977731979594082511211143919042824582762167539131463977851866139077527142735071061981353008559292148691719825214511577281414448340732036962232834959NCBITaxon:1167612296211634342911045375451367830308678495237109467989558015802362726588988