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rchive/2016/09/PXD004106/Thuricin17Salt1.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004106/LCOSalt1.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004106/LCO1.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004106/ControlSalt3.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004106/ControlSalt2.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004106/LCOSalt2.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004106/Control3.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004106/Thuricin173.fastaprimaryOK200003740donald.smith@mcgill.caSowmyalakshmi SubramanianMass SpectrometryShotgun proteomicsNot availableThuricin17Salt stressGerminationLc-ms/msSoybeanLipo-chitooligosaccharidehttp://www.ebi.ac.uk/pride/archive/projects/PXD004106Plant CellSeedFor the proteome analysis, germinated seeds from the unstressed condition, including the water-only control, 10-6 M LCO, 10-9 M Th17, and the salt stressed condition, including 100 mM NaCl as the salt-stressed control, and combinations of 100 mM NaCl with 10-6 M LCO and 10-9 M Th17, were sampled and total proteins extracted using a protein extraction kit (Cat. no. PE-0230, Plant total protein extraction kit, Sigma-Aldrich, Co., St. Louis, MO, USA). The total proteins extracted were then digested with trypsin and subjected to LC-MS/MS using a Velos Orbitrap instrument (Thermo Fisher, MA, USA). Tandem mass spectra were extracted, charge state deconvoluted and deisotoped and all MS/MS samples were analyzed using Mascot (Matrix Science, London, UK; version 2.3.02). Mascot was set up to search the Soybean_20120801 database (unknown version, 80416 entries) assuming the digestion enzyme trypsin. Mascot searched with a fragment ion mass tolerance of 0.60 Da and a parent ion tolerance of 15 PPM. Carbamidomethyl of cysteine was specified in Mascot as a fixed modification. Oxidation of methionine was specified in Mascot as a variable modification.PrideSpectrum count/molecular weightiodoacetamide derivatized residueScaffold (version Scaffold 4), Proteome Software Inc., Portland, OR), was used to validate MS/MS based peptide and protein identifications. Peptide identifications were accepted if they could be established at greater than 95.0 % probability, as specified by the Peptide Prophet algorithm. Protein identifications were accepted if they could be established at greater than 99.0 % probability and contained at least 2 identified peptides. Protein probabilities were assigned by the Protein Prophet algorithm. Proteins that contained similar peptides, and could not be differentiated based on MS/MS analysis alone, were grouped to satisfy the principle of parsimony. Scaffold 4 was used to analyze the proteomics data for fold change and Fisher’s exact test of the identified proteins, after subjecting the quantitative value of the spectra to the embedded normalization. The FASTA file generated was analyzed using Blast2GO-Pro 3.1.3, for the functional annotation and analysis of the protein sequences. Apart from these, Enzyme code (EC), KEGG maps and InterPro motifs were queried directly using the InterProScan web service.ProteomicsDonald L SmithLTQ Orbitrap VelosPARTIALJames McGill Professor Director and CEO, BioFuelNet Canada Director, McGill Network for Innovation in Biofuels and Bioproducts Director, Eastern Canadian Oilseeds Development Initiative Plant Science Department, McGill University 21111 Lakeshore Road Ste. Anne de Bellevue, Quebec Canada H9X3V9 Phone: +1 514 398 7866 Fax: +1 514 398 7990 Website: www.mcgill.ca/plant/faculty/smith/Glycine Max27560934 Subramanian S, Ricci E, Souleimanov A, Smith DL. A Proteomic Approach to Lipo-Chitooligosaccharide and Thuricin 17 Effects on Soybean GerminationUnstressed and Salt Stress. PLoS One. 2016 Aug 25;11(8):e0160660. eCollection 2016 10.1371/journal.pone.0160660sowmyalakshmi.subramanian@gmail.comBiologicalMcGill UniversityCanadaSalt stress is an important abiotic stressor affecting crop growth and productivity. Of the 20 percent of the terrestrial earth's surface available as agricultural land, 50 percent is estimated by the United Nations Environment Program to be salinized to the level that crops growing on it will be salt-stressed. Increased soil salinity has profound effects on seed germination and germinating seedlings as they are frequently confronted with much higher salinities than vigorously growing plants, because germination usually occurs in surface soils, the site of greatest soluble salt accumulation. The growth of soybean exposed to 40 mM NaCl is negatively affected, while an exposure to 80 mM NaCl is often lethal. When treated with the bacterial signal compounds lipo-chitooligosaccharide (LCO) and thuricin 17 (Th17), soybean seeds (variety Absolute RR) responded positively at salt stress of up to 150 mM NaCl. Shotgun proteomics of unstressed and 100 mM NaCl stressed seeds (48 h) in combination with the LCO and Th17 revealed many known, predicted, hypothetical and unknown proteins. In all, carbon, nitrogen and energy metabolic pathways were affected under both unstressed and salt stressed conditions when treated with signals. PEP carboxylase, Rubisco oxygenase large subunit, pyruvate kinase, and isocitrate lyase were some of the noteworthy proteins enhanced by the signals, along with antioxidant glutathione-S-transferase and other stress related proteins. These findings suggest that the germinating seeds alter their proteome based on bacterial signals and on stress, the specificity of this response plays a crucial role in organ maturation and transition from one stage to another in the plants' life cycle; understanding this response is of fundamental importance in agriculture and, as a result, global food security. The mass spectrometry proteomics data have been deposited to the ProteomeXchange with identifier PXD004106.A Proteomic Approach to Lipo-Chitooligosaccharide and Thuricin 17 Effects on Soybean GerminationUnstressed and Salt Stress.Subramanian Sowmyalakshmi S, Ricci Emily E, Souleimanov Alfred A, Smith Donald L DLliquid chromatography tandem mass spectroscopy, IPP2A2, determination, Pflanze, instrument configuration, Cs-1, protein, Xkl-1, viridiplantae, 5730420M11Rik, protein polypeptide chains, KL receptor activity, L-Isomer Methionine, halite, Gsfsco1, Drug Tolerance, Methionine, diseases, domestic cat, ionic compounds, SCO5, Msal-1, diseases and disorders, SCO1, Elaiosome, protein aggregate, Gsfsow3, Gsfsco5, 2210418N07, bacterial catalase-peroxidase activity, WMS, SOW3, C79691, SET, human disease, LCMSMS, reference sample, TAF-I, CT21282, M, phosphatase 2A inhibitor I2PP2A, Salt, Plant Embryo, Msal, proteins, W, Salz, sel, salts, Lccp, DmelCG4299, cloruro sodico, salt, set, IGAAD, haem catalase activity, DmelCG10574, Diaspores, ppm, Bs, Homo sapiens disease, Half Cystine, Sl, GPHYSD2, z, Racemethionine, drug tolerance, SGS, AI315345, catalase-peroxidase activity, LC-MS2, phapii, equilase activity, rock salt, Zinc Cysteinate, immune system tolerance, LC-MS/MS, 2-Amino-4-(methylthio)butyric acid, StF-IT-1, Tr-kit, 2.3.1.7, AI195249, A330009E03Rik, ACMICD, PBT, common salt, catalase reaction, kl1-A, Immunologic Tolerance, Methionin, methionine, Seed, Hmet, KIT, GAT, Hydrogen Oxide, ACGNAT, parent ion, tyrosine-protein kinase Kit, pbt, Diaspore, HLA-DR-associated protein II, Spalt, instrument, DI-2, Phaseolus max, ionic compound, I-2Dm, hydrogen-peroxide:hydrogen-peroxide oxidoreductase activity, Plant, Plant Zygote, MASCOT, L-Cysteine, kit, MASS, CG4299, inhibitor of granzyme A-activated DNase, natrii chloridum, Felis silvestris catus, beta Trypsin, I-2PP1, cv. Wye, disease, TAF-IBETA, Self Tolerance, Tolerance, precursor ion, liquid chromatography-tandem mass spectroscopy, TAF-Ibeta, DROCATHPO, i2pp2a, Liquimeth, Proteomes, bs36h11.y1, U00145, krk1, other disease, Cas1, Carnitine acetylase, Cysteine Hydrochloride, 2-amino-4-(methylsulfanyl)butanoic acid, AW549739, SCF receptor activity, L Isomer, FBN, Gene, precursor, protein-containing complex, PHAPII, LC-MS-MS, CrAT, manganese catalase activity, polypeptide chain, scfr, template-activating factor I, ECTOL1, LC-MSMS, Gene Products, disease or disorder, L-Methionine, caperase activity, Half-Cystine, SCFR, Pedameth, plantae, Fdc, L Cysteine, soybeans, ipp2a2, beta-Trypsin, chlorure de sodium, CatA, CATA, 2pp2a, Zygote, Sciences, CG10574, C79691., non-neoplastic, OCTD, soybean, mKIAA0989, DmelCG6871, 2PP2A, 2-amino-4-(methylthio)butanoic acid, 10^[-6], Catl, taf-ibeta, sels, Kochsalz, dSET, dSet, Zygotes, disorder, cats, metionina, Controlled, Elaiosomes, proto-oncogene c-Kit, Controlling, DMCATHPO, heme catalase activity, DL-Methionine, protein complex, sales, Proteins, disorders, igaad, total expressed protein, medical condition, L-Isomer, Carnitine acetyltransferase, LC/MS/MS, native protein, Immune Tolerance, Natriumchlorid, natural protein, I-2PP2A, KIT ligand receptor activity, Embryos, Felis domesticus, chemical analysis, Protein, Dm I-2, I2PP2A, condition, table salt, MFS1, CAT, NaCl, WMS2, Data Base, Met, XKrk1, Sal, optidase activity, CS1, Embryo, c-KIT, Cas-1, Tripcellim, B130022O04Rik, Salze, CG6871, cat, CD117, Plant Embryos, sal, Protein Gene Products, Gene Proteins, Trypure, dSET/TAF-Ibeta, c-kit, 2610030F17Rik, C-Kit, Plant Zygotes, Cat01, Dietary Sodium, Ssm, Glycine max, SSKS, xkl-1, alpha-amino-gamma-methylmercaptobutyric acid, liquid chromatography tandem mass spectrometry, assay, AA407739, sodium chloride, Immunological ToleranceSalinity Stresses, Stress Response, chitooligosaccharide, Phaseolus max, soybeans, Salinity Stress, Salt, Salt Stresses, thuricin S, BABL, Stress Reponse, Reponse, STQTL9, Salt Stress, LIPO, soybean, cv. Wye, Salinity Stress Reponses, Salinity Stress Reaction., HMGI-C, HMGIC, Stress Reaction, Reaction, thuricin 17, Glycine max, Salt Stress Reaction, Stress, Salt Stress Reactions, Salinity, Response, Salt Stress Response, Salt Stress Responses, Salinity Stress Reponse, Salinity Stress Reactions, ThuricinApplications Software, Open Source, data, Computer Software, determination, Peptidomics, protein complex, Ass-1, Proteins, total expressed protein, Gene, protein, Computer, protein-containing complex, Source Softwares, Peptide, Software Tools, Tool, Programs, polypeptide, peptide, Program, Computer Applications, Polypeptides, protein polypeptide chains, Software Tool, ClvPrd, peptido, native protein, natural protein, polypeptide chain, Algorithm, AA408052, Computer Applications Software, Protein, chemical analysis, Computer Applications Softwares, Gene Products, fold, Software Engineering, Softwares, protein aggregate, Software, Computer Program, Application, Maps., Open Source Softwares, Software Applications, peptides, Source Software, Software Application, Open, Engineering, Open Source Software, Computer Programs and Programming, proteins, Probabilities, Computer Software Application, Protein Gene Products, Computer Programs, ASS, Gene Proteins, Applications, Applications Softwares, Tools, Peptid, peptidos, Polypeptide, assay, Proteomes, Computer Software Applications70zpep, PEP/X4, Salinity Stresses, PP14, DmelCG30170, LYP2, b(2)gcn, LYP1, postnatal development, Mbp1, growth and development, Reponse, DmelCG6143, LIPO, Readability, halite, diseases, Carbon-12, Roles, responsivity, ionic compounds, gamma-L-Glutamyl-L-cysteinyl-glycine, Pop, symptoms, Salt Stress Response, Concepts, Msal-1, diseases and disorders, Elaiosome, myd, oxidoreductase activity, LyP, C, miscellaneous, human disease, Glycine, entire life cycle, N, Reduced, 5-L-Glutamyl-L-cysteinylglycine, Salt, Salt Stresses, acting on single donors with incorporation of molecular oxygen, Plant Embryo, Mbp-1, Msal, X4/PEP, proteins, plants, PEPPER, Pyruvate Kinase L, Salz, sel, salts, Glutathione, Reduced glutathione, cloruro sodico, LYP, salt, reaction, HMGIC, Lymphoid phosphatase, L-Type Pyruvate Kinase, L-Type, Diaspores, PrEP, Reaction, Carbon, Role Concepts, Homo sapiens disease, stage, Salinity Stress Reponse, PAEG, F12B17_170, fs(2)eo-C, screening, rock salt, Germinations, CG6143, gyltl1b-b, land plants, isocitrate lyase, ben(2)gcn, gamma-L-Glu-L-Cys-Gly, PEPINO, predicted, Salinity Stress Reaction, Vitreous, common salt, L Type Pyruvate Kinase, Role Concept, Stickstoff, MDDGA6, thuricin 17, mKIAA0609, 3.1.3.48, Role, Seed, fg, Diaspore, PEST-domain phosphatase, antioxidants, Stress Response, bcgn, gyltl1b, chitooligosaccharide, F20B18.110, Spalt, growth pattern, Phaseolus max, ionic compound, non-developmental growth, 6C, life, mdc1d, Plant, F20B18_110, expanded, signs, Plant Zygote, Isocitrate glyoxylate-lyase, AI047692, BABL, STQTL9, exposed, natrii chloridum, Foods., GD, M-Type, GSH, M1-Type Pyruvate Kinase, cv. Wye, Salinity Stress Reponses, disease, MDC1D, Vitreous Carbon, GdA, higher plants, nitrogen, GdF, enr, Agricultural, PNBP, enlarged, carbonium, Salt Stress Reactions, M2 Type Pyruvate Kinase, 7N, GdS, Salinity Stress Reactions, Thuricin, PEP, cg10331, Proteomes, big, R-Type Pyruvate Kinase, Agronomies, other disease, lifespan, Peptidomics, Salinity Stress, antoxidant, developmental stage, gcn, Gene, antioxydant, carbon, gamma-L-Glutamyl-L-Cysteinylglycine, Agronomy, Development, pep, froggy, Gyltl1a, fs(2)eo6, N-(N-L-gamma-glutamyl-L-cysteinyl)-, large, Transferase, CG30170, Salt Stress Reaction, Gene Products, disease or disorder, Farming, study, oxygenase, reactivity, entire lifespan, MDDGB6, soybeans, chlorure de sodium, Zygote, N-(N-gamma-L-Glutamyl-L-cysteinyl)glycine, M Type Pyruvate Kinase, Salt Stress, CG10331, CG10330, soybean, non-neoplastic, gamma L Glutamyl L Cysteinylglycine, BPFD#36, Kohlenstoff, extruding from, Stress Reaction, PASTICCINO 2, sels, great, gamma L Glu L Cys Gly, Kochsalz, BGCN, Zygotes, disorder, L-gamma-glutamyl-L-cysteinylglycine, R-Type, M1-Type, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, Elaiosomes, M-Type Pyruvate Kinase, findings, sales, exits through, Bgcn, Proteins, disorders, D10Wsu136e, total expressed protein, b(2)cgn, Isocitrase, M2-Type, medical condition, carbone, Pyruvate Kinase, Concept, carbono, development, HMGI-C, Natriumchlorid, Embryos, M1 Type Pyruvate Kinase, Protein, Oxygenase, condition, p100, table salt, Isocitrate, NaCl, Reduced Glutathione, M2-Type Pyruvate Kinase, Sal, Isocitratase, Lyase, Embryo, F12B17.170, Ptpn8, R Type Pyruvate Kinase, postnatal growth, B130022O04Rik, fs(2)eoQS2, Salze, thuricin S, Stress Reponse, azote, Understanding, Plant Embryos, sal, Carbon 12, nitrogeno, Protein Gene Products, Gene Proteins, Plant Zygotes, Dietary Sodium, Glycine max, Stress, Salinity, Response, Salt Stress Responses, Glutathione-SH, PE, response, AI450383, PTPN8, growth, Agricultural Development, sodium chloride70zpep, PEP/X4, Salinity Stresses, PP14, DmelCG30170, LYP2, b(2)gcn, LYP1, postnatal development, LUC7A, Mbp1, growth and development, Reponse, DmelCG6143, binding site, LIPO, Productivity, Readability, %, halite, Carbon-12, Roles, UNICEF, responsivity, ionic compounds, gamma-L-Glutamyl-L-cysteinyl-glycine, Pop, symptoms, Salt Stress Response, Concepts, Impacts, Msal-1, Analysis, Environmental Impacts, Elaiosome, myd, oxidoreductase activity, integumentum commune, Mass Spectrum Analysis, LyP, increased, C, miscellaneous, Malted Grain, Grain, Analyses, Glycine, entire life cycle, N, Reduced, 5-L-Glutamyl-L-cysteinylglycine, Salt, Salt Stresses, acting on single donors with incorporation of molecular oxygen, Plant Embryo, Mbp-1, Msal, X4/PEP, plants, proteins, PEPPER, Pyruvate Kinase L, Estimated, Economic and Social Commission for Asia and the Pacific, Salz, sel, salts, Glutathione, Reduced glutathione, cloruro sodico, Environmental Impact, LYP, salt, reaction, HMGIC, Lymphoid phosphatase, L-Type Pyruvate Kinase, L-Type, Diaspores, PrEP, data., Reaction, Carbon, Role Concepts, Sprouted, stage, Salinity Stress Reponse, PAEG, F12B17_170, fs(2)eo-C, screening, rock salt, Germinations, CG6143, external covering of organism, PLATEST, gyltl1b-b, CROP, land plants, Crop, isocitrate lyase, ben(2)gcn, gamma-L-Glu-L-Cys-Gly, organism surface, UN, PEPINO, Spectrum Analysis, predicted, Salinity Stress Reaction, Programs, Spectroscopy, Vitreous, common salt, Plant Sprouts, L Type Pyruvate Kinase, Role Concept, soluble, Stickstoff, MDDGA6, thuricin 17, mKIAA0609, 3.1.3.48, Role, Seed, region, fg, Seeds, Diaspore, PEST-domain phosphatase, antioxidants, Stress Response, bcgn, gyltl1b, positional polypeptide feature, chitooligosaccharide, F20B18.110, growth pattern, Spalt, craw, Phaseolus max, ionic compound, non-developmental growth, 6C, life, CREAP-1, mdc1d, Plant, F20B18_110, expanded, Spectrometry, signs, pooled, Plant Zygote, Isocitrate glyoxylate-lyase, AI047692, BABL, exposed, STQTL9, natrii chloridum, GD, M-Type, GSH, M1-Type Pyruvate Kinase, Salinity Stress Reponses, cv. Wye, Seedling, MDC1D, Grains, Vitreous Carbon, higher plants, GdA, nitrogen, GdF, enr, Agricultural, PNBP, enlarged, integumentary system, carbonium, Salt Stress Reactions, M2 Type Pyruvate Kinase, 7N, GdS, Salinity Stress Reactions, Thuricin, dermal system, PEP, cg10331, Proteomes, Platelets, accessory, United Nations Children's Fund, hLuc7A, big, Plant Sprout, R-Type Pyruvate Kinase, Agronomies, lifespan, region or site annotation, Peptidomics, Salinity Stress, antoxidant, developmental stage, gcn, Gene, antioxydant, carbon, gamma-L-Glutamyl-L-Cysteinylglycine, Agronomy, Development, Spectrum Analyses, pep, supernumerary, froggy, Gyltl1a, fs(2)eo6, N-(N-L-gamma-glutamyl-L-cysteinyl)-, surface, large, Transferase, CG30170, Salt Stress Reaction, Peat, Sprouted Seed, Gene Products, Mass, Malted, RGD1307981, Mass Spectroscopy, Farming, oxygenase, reactivity, positional, entire lifespan, MDDGB6, soybeans, United Nations High Commissioner for Refugees (UNHCR), chlorure de sodium, Humus, Zygote, N-(N-gamma-L-Glutamyl-L-cysteinyl)glycine, M Type Pyruvate Kinase, Salt Stress, CG10331, CG10330, soybean, gamma L Glutamyl L Cysteinylglycine, BPFD#36, Kohlenstoff, extruding from, Stress Reaction, PASTICCINO 2, sels, great, gamma L Glu L Cys Gly, Kochsalz, BGCN, Soils, Zygotes, site, L-gamma-glutamyl-L-cysteinylglycine, R-Type, M1-Type, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, Elaiosomes, M-Type Pyruvate Kinase, findings, Malted Grains, Latin American Demographic Center, sales, exits through, Bgcn, OA48-18, Proteins, D10Wsu136e, total expressed protein, b(2)cgn, Isocitrase, M2-Type, Sprout, carbone, Pyruvate Kinase, Impact, Concept, carbono, development, Environmental, HMGI-C, Natriumchlorid, UNHCR, Embryos, M1 Type Pyruvate Kinase, Protein, Oxygenase, p100, CRA, table salt, Isocitrate, ESCAP, NaCl, Reduced Glutathione, M2-Type Pyruvate Kinase, Foods, Mass Spectrum Analyses, Sal, Isocitratase, Lyase, Embryo, Mass Spectrum, F12B17.170, Sprouts, Ptpn8, body surface, increased number, INSDC_feature:misc_binding, R Type Pyruvate Kinase, postnatal growth, B130022O04Rik, fs(2)eoQS2, Salze, thuricin S, Stress Reponse, azote, Understanding, Plant Embryos, sal, Carbon 12, nitrogeno, Protein Gene Products, present in greater numbers in organism, Gene Proteins, binding_or_interaction_site, Plant Zygotes, Dietary Sodium, Glycine max, Stress, Salinity, Sprouted Seeds, Response, Environments, Salt Stress Responses, Glutathione-SH, PE, response, earth, AI450383, PTPN8, growth, Agricultural Development, sodium chloridesoybean, liquid chromatography tandem mass spectroscopy, LC-MS2, cv. Wye, LC/MS/MS, LCMSMS, Phaseolus max, liquid chromatography-tandem mass spectroscopy, Glycine max, LC-MSMS, soybeans, LC-MS/MS, liquid chromatography tandem mass spectrometry, LC-MS-MS.374trueSoybean seed germination LC-MS/MSThis study aims at addressing soybean seeds (variety Absolute RR) germination, at 48h, during optimal and salt stressed condition when treated with bacterial signal compounds lipo-chitooligosaccharide (LCO) and thuricin 17 (Th17). Soybean growth is negatively affected when exposed to 40 mM NaCl and exposure to 80 mM NaCl is often lethal. When treated with the bacterial signal compounds lipo-chitooligosaccharide (LCO) and thuricin 17 (Th17), soybean seeds (variety Absolute RR) responded positively at salt stress of up to 150 mM NaCl. Shotgun proteomics of unstressed and 100 mM NaCl stressed seeds (48 h) in combination with the LCO and Th17 revealed many known, predicted, hypothetical and unknown proteins. In all, carbon, nitrogen and energy metabolic pathways were affected under both unstressed and salt stressed conditions when treated with signals. PEP carboxylase, Rubisco oxygenase large subunit, pyruvate kinase, and isocitrate lyase were some of the noteworthy proteins enhanced by the signals, along with antioxidant glutathione-S-transferase and other stress related proteins. These findings suggest that the germinating seeds alter their proteome based on bacterial signals and on stress, the specificity of this response plays a crucial role in organ maturation and transition from one stage to another in the plants life cycle; understanding this response is of fundamental importance in agriculture and, as a result, global food security.2016-09-122016-05-05PXD00410644664499175582580388043330295358511145108061246196246197320637581136335090683635275838538791187947398007NCBITaxon:1280572307111430155571468351452623649236411354613885803582338886915688953997844751870435112329469281239771331034304365481358339251981836592198131432059655819149363295726373769381247818661328388281395451358672229491408169180454161934139036345301204162326442104117061274432108595614963674283166694369455972911320810039237514633087936944784208964317447127044597411946691454587932993529320101981264690410661145755121659939251216597216595160621213312831282128033952321832172920435315228112454349825747874980197525555362312951053948824261914548140479NCBITaxon:215755153439899037120596876231935163098005888455818178081736309431796989361604885661409859566416242515059071187021187053204963063863235554320466322261866331299456586068845351216129567171360441001602358700264203694569410167634102107612773512793342814610029357861187002157641809699745774449156666754760445246763366411363367102398709NCBITaxon:405592359092788396526498217498216347256569156934982116544872056971089448663661410NCBITaxon:493287155689102451000589452680873231156126373039114155421932555218726872387248729872755529547624223141463055180066556484198822299767982510216998231174673897641155744689444474153NCBITaxon:50557960167767446859600653491182263129911457845863267656983556636983819201124136810893161001428099612NCBITaxon:548681NCBITaxon:5820546414307747946409860995865228319662776689605960615729513722187594467087562270861711013354834974138783430615470334249961588655974649987039202187827215407821190857075206189480418985350247158779627190252867463609646375939146247274612874601134506253452659197221963852638588596NCBITaxon:6157110517237263419610526419407722774694163481123869NCBITaxon:270361274767927459615325731326979699034694171344346360347515363296440421097606331492862879669968583558595968912801726071018568729411009118696141633316780781186941436183NCBITaxon:6191118698840231719118691443906NCBITaxon:10298210007969419080218787870384766455347095145122578656870861822607046183260705260707NCBITaxon:780NCBITaxon:1502451516658457702921158612184520928574707861921263854504457383379192875NCBITaxon:10239NCBITaxon:71428291268063130957044830064110555241590841228233446900815025754122810430085210977970671356225833136185509924NCBITaxon:56938268830640527796583342409061510151540674482428130767070876138370828167728509901195195337024924053608881756261533376021267192853217735726711935018464517723711121532369014726647091571256274579031133355085962859634792491308212035332648373153NCBITaxon:96153185861412622002984937608333483332101841100658123756126662557492003600941530094114903524725175014012573983649908515151438992NCBITaxon:11552374537474454464152593117928664323598390628870515801194599327160114978616032932853422999810894535936457428135122511775058NCBITaxon:1040716797181673871349158910306502780291595412629158515849327159570210022612876891355889309451464792031242164133280382803519284343985808946217939211825902878892950279069017493427256322732189038292867124719040072753745711431123352114020641198108713554771424507229533355571415429315754622421392139011431933829355083329230741129221148489672526499975744727580240550717629936658121405357622941281607839576320667240054029654318431832246575549922957575759284593307972113549706671436733115955644421432138184922311210969762261111795752981818431923347986626528104660454747962678253567097477317513565050NCBITaxon:131360769938942468879552014887519795962141421972890922853164351867631854315206494089414424232NCBITaxon:177321409211577892979324470420755910117749906749907312989913112273170187367451011610358132842626587210360983341392696113079813367953588931640710340153033373301581999253068839103372123368199404458611535711471612249113430531201755499351299385057317666964268845151345842157267872862386211151041607912983154689119835112552285625323213616763363168970413288818855116761080772326424861839376520965731116234273068808637721666898626NCBITaxon:2697049339862410312NCBITaxon:13899758644864331972917634386372567378639123343510523110090514140544931799868655405534865451471245466760568104298635712467918840193733450877119821597397574297194275779850762554702563188657623951655865886223864946454634076NCBITaxon:1107128835649184588NCBITaxon:5920114594326767111167810774084408797789534967944081226900380394351281459536123517861624323039966869597854096409795418694200308954424327786976279129249954540905180426430409286924093200302284812313625426428150475102993886538626290341554051208979662877323966920297936289955771371170089306218644195486575584628238235217362315577223994636010688420436010439947932184795444044731169681121279011622961731440772131693193128118361003610502318557984854318637499010041715916922596345910659210659033454288531844271716591037164784964102896883331249668425281094343197NCBITaxon:10357NCBITaxon:10114507601NCBITaxon:1035927263495602959880224275295952664373235443224308143361943274983964271191364516996311222336052012169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