Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4043_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4039_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4055_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4031_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4047_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4051_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4027_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4035_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4015_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150724_QE5_UPLC8_RJC_COLLAB_DB_4127_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150724_QE5_UPLC8_RJC_COLLAB_DB_4099_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150724_QE5_UPLC8_RJC_COLLAB_DB_4119_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4023_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150724_QE5_UPLC8_RJC_COLLAB_DB_4131_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150724_QE5_UPLC8_RJC_COLLAB_DB_4107_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150724_QE5_UPLC8_RJC_COLLAB_DB_4115_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150724_QE5_UPLC8_RJC_COLLAB_DB_4123_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4011_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150724_QE5_UPLC8_RJC_COLLAB_DB_4103_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150724_QE5_UPLC8_RJC_COLLAB_DB_4095_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4071_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4079_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4075_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4019_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4063_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4087_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4083_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4091_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4067_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/20150722_QE5_UPLC8_RJC_COLLAB_DB_4059_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004319/Saliva_txt.rarprimaryOK200104650jesper.olsen@cpr.ku.dkRosa Jersie-ChristensenMass SpectrometryShotgun proteomicsNot availableHumanMetaproteomicsQ exactive hfSalivahttp://www.ebi.ac.uk/pride/archive/projects/PXD004319SalivaThe saliva samples were prepared as described in (Jersie-Christensen et al., 2016) with a few modifications. In short, 1ml of saliva was mixed with 1.5ml lysis buffer (9M Guanidine hydrochloride, 10mM Chloroacetamide, 5mM tris(2-carboxyethyl)phosphine in 100mM Tris pH 8.5) and heated for 10min (99°C) followed by 4min of sonication. All samples were digested with Lysyl Endoproteinase in a ratio of 1:100 for 2hrs and diluted and digested overnight with Trypsin in a 1:100 ratio. 1.5µg peptide mixture was separated on an in-house packed 50 cm capillary column with 1.9 μm Reprosil-Pur C18 beads using an EASY-nLC 1000 system (Thermo Scientific). The column temperature was maintained at 50°C using an integrated column oven. The gradient was 155min The Q Exactive HF instrument (Thermo Scientific, Bremen, Germany) was run in a data dependent top 12 mode with the following settings. Spray voltage was set to 2 kV, S-lens RF level at 50, and heated capillary at 275 °C. Full scan resolutions were set to and 60 000 at m/z 200 and scan target was 3 × 10E6 with a maximum fill time of 20 ms. Mass range was set to 300–1750 and dynamic exclusion to 20 s. Target value for fragment scans was set at 1 × 105 with a resolution of 30 000 and a maximum fill time of 60 ms. Normalized collision energy was set at 28.PrideNot availableNo PTMs are included in the datasetRaw data were processed using MaxQuant version 1.5.0.38 (Cox and Mann, 2008) with default settings and 1% FDR at both peptide and protein level and with match between runs. The data was searched against a reduced combined fasta consisting of the full SwissProt database and the Human Oral Microbiome database (both downloaded August 2014). Intensities were Quantile normalized using the limma package version 3.24.15 with R version 3.2.2. Only proteins identified with more than one peptide (“razor+unique”) and present in more than five out of the 30 samples were considered for further analysis.ProteomicsJesper Velgaard OlsenQ ExactivePARTIALUniversity of Copenhagen Faculty of Health and Medical Sciences Novo Nordisk Foundation Center for Protein Research Proteomics ProgramHomo Sapiens (human)rosa.rjc@cpr.ku.dk27672500 Belstrøm D, Jersie-Christensen RR, Lyon D, Damgaard C, Jensen LJ, Holmstrup P, Olsen JV. Metaproteomics of saliva identifies human protein markers specific for individuals with periodontitis and dental caries compared to orally healthy controls. PeerJ. 2016 Sep 14;4:e2433. eCollection 2016 10.7717/peerj.2433BiologicalBiomedicalUniversity of CopenhagenDenmark<h4>Background</h4>The composition of the salivary microbiota has been reported to differentiate between patients with periodontitis, dental caries and orally healthy individuals. To identify characteristics of diseased and healthy saliva we thus wanted to compare saliva metaproteomes from patients with periodontitis and dental caries to healthy individuals.<h4>Methods</h4>Stimulated saliva samples were collected from 10 patients with periodontitis, 10 patients with dental caries and 10 orally healthy individuals. The proteins in the saliva samples were subjected to denaturing buffer and digested enzymatically with LysC and trypsin. The resulting peptide mixtures were cleaned up by solid-phase extraction and separated online with 2 h gradients by nano-scale C18 reversed-phase chromatography connected to a mass spectrometer through an electrospray source. The eluting peptides were analyzed on a tandem mass spectrometer operated in data-dependent acquisition mode.<h4>Results</h4>We identified a total of 35,664 unique peptides from 4,161 different proteins, of which 1,946 and 2,090 were of bacterial and human origin, respectively. The human protein profiles displayed significant overexpression of the complement system and inflammatory markers in periodontitis and dental caries compared to healthy controls. Bacterial proteome profiles and functional annotation were very similar in health and disease.<h4>Conclusions</h4>Overexpression of proteins related to the complement system and inflammation seems to correlate with oral disease status. Similar bacterial proteomes in healthy and diseased individuals suggests that the salivary microbiota predominantly thrives in a planktonic state expressing no disease-associated characteristics of metabolic activity.Metaproteomics of saliva identifies human protein markers specific for individuals with periodontitis and dental caries compared to orally healthy controls.Belstrøm Daniel D, Jersie-Christensen Rosa R RR, Lyon David D, Damgaard Christian C, Jensen Lars J LJ, Holmstrup Palle P, Olsen Jesper V JVscale tissue, DENT WHITE SPOT, Inflammations, Activity, Feature, WHITE SPOTS DENT, mouth disease, Cavity, composed of, peptide, Polypeptides, solid, Techniques, ClvPrd, peptido, diseases, Method, Pericementitides, B1, WHITE SPOT DENT, diseases and disorders, Dentin, average, Dental Cavity, Divorced, human disease, inflammatory response, peptides, plant peltate hair, Microbial Community Structure, composition, proteins, Divorces, procedures, dental caries of smooth surface, Methodological Studies, White Spot, D1, Dental White Spots, Microbial Community Structures, Dental caries, Homo sapiens disease, Decay, associated, inflammation of periodontium, Tooth Decay, oral cavity disorder, Salivas, Normalcy, Procedure, Microbial, Periodontitis (disorder), results, disease of the mouth, DENT CARIES, Dental Cavities, Diseases, CARIES DENT, NOS, Individual, periodontitis, Lesion, Microbial Communities, Carious Lesions, Microbial Community Compositions, content, DECAY DENT, DENT DECAY, Methodological, Features, Methodological Study, beta Trypsin, organ system, Composition, disease, Health, Patient, disease or disorder of mouth, DENT WHITE SPOTS, oral cavity disease, White, Dental Caries, Polypeptide, Innate, Proteomes, Human Microbiomes, oral disease, Community, caries, Microbial Community Composition, periodontosis, other disease, Inflammatory Response, Spot, Procedures, Periodontitides, peltate hair, Gene, Normalcies, body system, oral disorder, Human, Buffer, Pericementitis, Carious Dentins, saliva molecularis, Studies, Gene Products, disease or disorder, system, Periodontitis, Separated, Technique, chronic pericementitis, Normalities, Caries, periodontium inflammation, anatomical systems, saliva atomaris, beta-Trypsin, General activity., inflammation, Spots, dental caries pit and fissure, Lesions, Dental, non-neoplastic, Study, White Spots, Innate Inflammatory Response, Clients, disease of mouth, scale (sensu Metazoa), disorder, Dental White Spot, peptidos, Characteristics, Chromatographies, Dental Decay, PTHB1, Human Microbiome, Individual Health, Community Composition, mouth disease or disorder, disorder of mouth, Proteins, Cavities, disorders, total expressed protein, medical condition, compositionality, backward, buffer, Client, Peptide, Dentins, polypeptide, Characteristic, C18, Protein, condition, background, techniques, scales, connected anatomical system, Microbiome, Carious Dentin, DDA, Separation, Microbiomes, Carious, scale, Normality, Microbial Community, Separations, Community Structure, Tripcellim, dental caries extending into pulp, introduction, mouth disorder, Protein Gene Products, Gene Proteins, Trypure, Carious Lesion, salivary gland secretion, Innate Inflammatory Responses, structure, dental caries, Microbiotas, Peptid, sailva normalis, methodology, reversed, Dental Whitesmall, Bru, data, cox4, human being, Raw, determination, protein complex, Proteins, Gene, protein, protein-containing complex, Peptide, polypeptide, peptide, CG10664, Polypeptides, protein polypeptide chains, ClvPrd, peptido, native protein, natural protein, polypeptide chain, reduced, VA, subnumerary, AL024441, IV, Protein, Gene Products, IV-1, Del(8)44H, tiny, COX IV, DmelCG10664, protein aggregate, present in fewer numbers in organism, Data Base, Svc, Coproporphyrinogenase, Col4a-1, peptides, underdeveloped, Va, COXIV, Coprogen oxidase, COX IV-1, tend, hypoplasia, chemical analysis., proteins, present in organism, decreased number, COXA_DROME, man, human, Protein Gene Products, Gene Proteins, CG14724, decreased, Cox4, COX, Peptid, peptidos, 1.3.3.3, DmelCG14724, Polypeptide, assay, COX-VA, Cox4aperiodontosis, inflammation of periodontium, Tooth Decay, Spot, DENT WHITE SPOT, Periodontitides, Cavities, WHITE SPOTS DENT, Salivas, Cavity, Periodontitis (disorder), DENT CARIES, Dentins, Dental Cavities, Pericementitides, Pericementitis, saliva molecularis, CARIES DENT, Carious Dentins, WHITE SPOT DENT, NOS, Periodontitis, periodontitis, Lesion, chronic pericementitis, Carious Dentin, Dentin, Carious Lesions., Caries, Dental Cavity, periodontium inflammation, Carious, saliva atomaris, DECAY DENT, DENT DECAY, dental caries extending into pulp, Spots, dental caries pit and fissure, Lesions, Dental, dental caries of smooth surface, White Spots, Carious Lesion, salivary gland secretion, White Spot, DENT WHITE SPOTS, Dental White Spots, dental caries, Dental caries, White, Dental White Spot, Dental Caries, sailva normalis, Decay, Dental Decay, Dental White, cariesperiodontosis, inflammation of periodontium, Tooth Decay, Spot, DENT WHITE SPOT, Periodontitides, Cavities, WHITE SPOTS DENT, Salivas, Cavity, Periodontitis (disorder), DENT CARIES, Dentins, Dental Cavities, Pericementitides, Pericementitis, saliva molecularis, CARIES DENT, Carious Dentins, WHITE SPOT DENT, NOS, Periodontitis, periodontitis, Lesion, chronic pericementitis, Carious Dentin, Dentin, Carious Lesions., Caries, Dental Cavity, periodontium inflammation, Carious, saliva atomaris, human protein, DECAY DENT, DENT DECAY, dental caries extending into pulp, Spots, dental caries pit and fissure, Lesions, Dental, dental caries of smooth surface, White Spots, Carious Lesion, salivary gland secretion, White Spot, DENT WHITE SPOTS, Dental White Spots, dental caries, Dental caries, White, Dental White Spot, Dental Caries, sailva normalis, Decay, Dental Decay, Dental White, cariesIPP2A2, Effects, chlorure d'hydrogene, instrument configuration, Lens <eudicots>, FBN, Monohydrate, Guanidine Sulfate (2:1), Hydrogenchlorid, lysis, thomson, body system, PHAPII, lens crystallina, Long Term, Buffer, mass-to-charge ratio, 5730420M11Rik, peptide, lenses, period, Polypeptides, Guanidium, hydrogen chloride, ClvPrd, peptido, template-activating factor I, ECTOL1, B1, saliva molecularis, system, camera-type eye lens, Separated, Effect, cloruro de hidrogeno, WMS, Guanidium Chloride, SET, proportion, Divorced, Monohydroiodine, anatomical systems, i2pp2a., peptides, Hydrogen chloride, TAF-I, Longterm, phosphatase 2A inhibitor I2PP2A, saliva atomaris, ipp2a2, beta-Trypsin, ocular lens, stubby, crystalline lens, 2pp2a, Phosphate, Divorces, Long-Term, SCAN, CG10574, DmelCG4299, OCTD, set, IGAAD, Wasserstoffchlorid, Monohydrobromide, Guanidinium Chloride, 2PP2A, column, DmelCG10574, taf-ibeta, D1, dSET, dSet, peptidos, Long-Term Effect, autolysin activity, necrosis, Guanidine Sulfate (1:1), blood capillary, GPHYSD2, eye lens, Guanidine Hydrochloride, Long-Term Effects, TCEP, PTHB1, SGS, ratio, phapii, Guanidine Monohydrate, data, Guanidine Nitrate, lens capsule, Monohydrochloride, igaad, Longterm Effect, Salivas, StF-IT-1, Th, Guanidine Monohydroiodine, chloridohydrogen, Mischung, buffer, Thermo Scientific, Guanidinium, Peptide, ACMICD, shortened, polypeptide, [HCl], capillary vessel, Temperatures, I-2PP2A, C18, Dm I-2, Long Term Effects, I2PP2A, Hydrochloride, Chlorwasserstoff, Guanidine Sulfite (1:1), bacteriocin activity, Nitrate, MFS1, connected anatomical system, WMS2, Guanidine Monohydrobromide, Separation, Guanidine, holin, Guanidine Sulfate, HLA-DR-associated protein II, instrument, DI-2, lens, Sonications, I-2Dm, Separations, HCl, proportionality, Tripcellim, Chloride, Guanidine Phosphate, Guanidine Monohydrochloride, rate, CG4299, MASS, inhibitor of granzyme A-activated DNase, beta Trypsin, Longterm Effects, chloracetamide, organ system, I-2PP1, Trypure, dSET/TAF-Ibeta, 2610030F17Rik, lysin activity, TAF-IBETA, salivary gland secretion, SSKS, bacteriolytic toxin activity, quotient, Peptid, TAF-Ibeta, sailva normalis, Polypeptide, chlorane, short, AA407739, i2pp2a, Sulfate, time, hydrochloric acid, Lens <bivalves>scale tissue, Biological Markers, Viral Marker, Bogalusa Heart Study, DENT WHITE SPOT, Surrogate Endpoints, Activity, Laboratory, WHITE SPOTS DENT, Biochemical, Mbp1, mouth disease, Cavity, Endpoint, Serum, Heart Study, composed of, Bogalusa Heart, Laboratory Markers, diseases, Biological, California, Pericementitides, WHITE SPOT DENT, California Teachers Study, diseases and disorders, myd, Bogalusa Heart Studies, Dentin, average, Dental Cavity, human disease, plant peltate hair, Microbial Community Structure, Mbp-1, composition, present in organism, proteins, dental caries of smooth surface, Immune, Markers, Viral Markers, White Spot, Dental White Spots, Microbial Community Structures, Dental caries, Homo sapiens disease, Syphilis Study, Decay, associated, inflammation of periodontium, Tooth Decay, Viral, Surrogate Endpoint, gyltl1b-b, Jackson, oral cavity disorder, Salivas, Longitudinal Survey, Biochemical Markers, Normalcy, Jackson Heart, Microbial, Biologic Marker, Periodontitis (disorder), Teachers Study, disease of the mouth, DENT CARIES, Longitudinal, Dental Cavities, Marker, MDDGA6, mKIAA0609, Diseases, CARIES DENT, NOS, Individual, periodontitis, Lesion, Microbial Communities, Carious Lesions, fg, gyltl1b, End Points, Microbial Community Compositions, content, mdc1d, expanded, DECAY DENT, DENT DECAY, Immunologic, Laboratory Marker, human, organ system, Composition, disease, MDC1D, Health, enr, Patient, enlarged, Biochemical Marker, disease or disorder of mouth, DENT WHITE SPOTS, oral cavity disease, Surveys, White, medical condition., Dental Caries, Oral Health, Human Microbiomes, oral disease, Tuskegee Syphilis Studies, Community, caries, big, Microbial Community Composition, periodontosis, other disease, Spot, human being, Periodontitides, Peptidomics, Clinical Markers, Clinical Marker, peltate hair, Gene, Normalcies, body system, oral disorder, froggy, Gyltl1a, Human, Surrogate End Points, Surrogate Markers, large, Pericementitis, Carious Dentins, saliva molecularis, Gene Products, Studies, Bogalusa, disease or disorder, Survey, system, Periodontitis, Longitudinal Surveys, chronic pericementitis, Normalities, Jackson Heart Studies, Caries, study, Biomarker, periodontium inflammation, anatomical systems, Framingham Heart Studies, Clinical, Oral, MDDGB6, Biological Marker, saliva atomaris, Spots, dental caries pit and fissure, man, Lesions, Dental, non-neoplastic, Jackson Heart Study, Study, BPFD#36, White Spots, Immunologic Markers, Clients, great, disease of mouth, disorder, scale (sensu Metazoa), Dental White Spot, Immunologic Marker, Dental Decay, Biologic, Human Microbiome, Oral health, Individual Health, data, Community Composition, mouth disease or disorder, California Teachers, Longitudinal Study, disorder of mouth, Teachers Studies, Proteins, Serum Markers, Cavities, disorders, End Point, California Teachers Studies, medical condition, Tuskegee, compositionality, Client, Immune Marker, Dentins, Heart Studies, Syphilis Studies, Surrogate End Point, Protein, proteomic analysis, condition, connected anatomical system, scales, Microbiome, Biologic Markers, Carious Dentin, Serum Marker, Microbiomes, Carious, scale, Normality, Microbial Community, Surrogate, Endpoints, Tuskegee Syphilis Study, Community Structure, dental caries extending into pulp, Framingham, Surrogate Marker, mouth disorder, Protein Gene Products, Gene Proteins, Framingham Heart Study, Carious Lesion, salivary gland secretion, structure, dental caries, Microbiotas, sailva normalis, General activity, Dental White, Immune Markers4651trueMetaproteomic profiling of saliva in subjects with periodontitis, dental caries and orally healthy controlsThe composition of the salivary microbiota has been reported to differentiate between patients with periodontitis, dental caries and orally healthy individuals. Thus, the purpose of the present investigation was to compare metaproteomic profiles of saliva in oral health and disease. Stimulated saliva samples were collected from 10 patients with periodontitis, 10 patients with dental caries and 10 orally healthy individuals. Samples were analyzed by means of shotgun proteomics. 4161 different proteins were recorded out of which 1946 and 2090 were of bacterial and human origin respectively. The human proteomic profile displayed significant overexpression of the complement system and inflammatory mediators in periodontitis and dental caries. Bacterial proteomic profiles and functional annotation were very similar in health and disease. Data revealed multiple potential salivary proteomic biomarkers of oral disease. In addition, comparable bacterial functional profiles were observed in periodontitis, dental caries and oral health, which suggest that the salivary microbiota predominantly thrives in a planktonic state expressing no characteristic disease-associated metabolic activity. Future large-scale longitudinal studies are warranted to reveal the full potential of proteomic analysis of saliva as a biomarker of oral health and disease.2016-08-152016-06-13PXD00431944664499175582580388043330295358108061246196246197320637581136335090683635275838538791187947398007NCBITaxon:128057230711143015557146835364923641135461388580338886915475187043511232946928123977133103430436548135833925198183659219813143205914936329572637376938124781866281395451329491408169180454161934139036345303264421041170612744321496367428316669436945597291003923751463308794784208964317447127011946697932993529312646904106611457551216599216595213312831282128033952321832172920435315228112454347874980197525555362313948824261914548140479NCBITaxon:2157551534398920596876233098005888455818178081736309431796989361604885661409859566416242515059071187021187053204963063863235554320466326331299456586068845351216129567171360441001602358700264203694569410167634102107612773512793310029118700215764180945774449156666754760445246763366411363367102398709NCBITaxon:4055923590927883965264982174982165691569349821187201089448663NCBITaxon:4932871510005898732311561263730391141554219328726872387248729872755529547624223141463055180066556484198822299767982510216998231174673897641155744689444474153960167767446859600118226312991145784586326983819201124136810893161001428099612NCBITaxon:548681546414307747946409860995865228319662776689605960615729513722187594467087562270861711013354834974138783430615470334249961574649987087827215407821190857075206189480418985350247158779627190252867463609646375939146247274612874601134506253197221963852638588596NCBITaxon:6157110517237263526419407722774691123869NCBITaxon:2703612747679274596153257313269796990346941713443347515363296440421097606331492862879669968583558595968926071029411009118696141633316780781186941436183NCBITaxon:6191118698840231719118691969419080218787870384766455347012257865687082607046183260705260707NCBITaxon:780NCBITaxon:1502451516658457702921158612184520928574707861921263854504457383379192875NCBITaxon:10239NCBITaxon:71428291268063704483006411055524159084122823344690081502575412281043008521097791356225833136185NCBITaxon:56938268830640527796583342409064067413076876138370828509901195195337024924081756261533376028532177311935018464537111215323690147091571256903113335508596285963332648373153NCBITaxon:9615141262200298493760833348333210065812375612666255749200360094352472517503983649908515151438992NCBITaxon:11552374537474454464152593117928664323598390628870511945993271601603293285342299981089453135122511775058167971816738713491589103062915954126291585158493271595702100226128768913558893094514647920312421641332803828035398580894621793921182590287889295027906901749342725632273218903829286712471904007275374571143114020641198108713554771424507229533355571415754622421392139011431933829355083329230741114848967252649997727580240550736658121405357622941282965431843183224657555759284593307972706671436733115955644421432138184922311210969762261111795752981833479866265284547467825317513565050NCBITaxon:1313607699389424688795520148875197959621412890922853164351867631854319414424232NCBITaxon:177321409292979320755910117749907991311227317018736745101161035813284262658721036098334139269613367953588931640710340153031581999253068839123368199404458611535711471612249113430531201755499351299331766696426884515134584215726787286238621115104160791298315468911983511255228562136167633631681328881885511676108077232642486183937652096573273068808637721666898626NCBITaxon:2697049339862410312NCBITaxon:1389975864486433197291768637256737863912334351052311009040544931799868655865410429863571246791884019373345087711982159739757429719985076255470256318623951655865886223864946454634076NCBITaxon:1107128835649184588NCBITaxon:59201145943111674084408797789534967944081226900380394351281459536123517861624323086959785409640979541869420030895442432778697627912924995454090518042643040928692409320030228481231362542642815047510299388653862629034155405120897966287732396289955711700893062186441954865755846282382352173623139946360106884204360104399479321847954440447311696811212790173144077293128118361003610502318557984853749901004116922596345910659210659033454288531844271716591037164784961249668425281094343197NCBITaxon:10357NCBITaxon:10114NCBITaxon:10359272634956029598802242752959526643732354432243081433619432749839642711913645169963112223312169792726232726241238993869728643680334747182131426970494032480302976038181147787127441415368163958885874940295546804922432640817212816140559204254638271274423434271408170127442012744264911543734131641660100971482962823525675060189519295214505114924956481882299673146051951876658002148015412045513847360338481423188294516631015101450520493227591428693732664179297221097677112111419110792762518665592924847914869179172261756614353407312032581205259447375451367830580127672500