Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/proteinGroups_AHA.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/peptides_AHA.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/modificationSpecificPeptides_AHA.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/peptides_SS.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/msms_AHA.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/modificationSpecificPeptides_SS.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/proteinGroups_SS.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/msms_SS.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150904_SEM_RPE1_2h_125mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20160128_SEM_Chrom_8_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150904_SEM_RPE1_32h_125mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150928_SEM_Chromosome_Light.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150908_SEM_RPE1_32h_Exp2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150904_SEM_chrom_32h_125mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20150908_SEM_chrom_8h_Exp2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150902_SEM_chrom_048_500mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20150908_SEM_RPE1_8h_Exp2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150908_SEM_RPE1_8h_Exp2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20160129_SEM_RPE_32_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20160127_SEM_Chrom_2_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150904_SEM_RPE1_32h_500mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20160127_SEM_Chrom_32_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20160128_SEM_RPE_32_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150907_SEM_Chrom_8h_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20160129_SEM_RPE_8_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20160129_SEM_RPE_2_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20160128_SEM_RPE_32_Exp4_160129160256.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20150908_SEM_chrom_2h_Exp2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150904_SEM_RPE1_8h_125mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20150908_SEM_RPE1_2h_Exp2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20160128_SEM_RPE_2_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150907_SEM_Chrom_32h_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150928_SEM_Chromosome_Heavy.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20160127_SEM_Chrom_8_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20150908_SEM_RPE1_32h_Exp2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150907_SEM_Chrom_2h_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150902_SEM_chrom_012_125mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20150908_SEM_chrom_32h_Exp2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20160128_SEM_RPE_8_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20160128_SEM_Chrom_2_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20160128_SEM_Chrom_32_Exp4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20150928_SEM_Chromosome_Heavy.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150904_SEM_RPE1_2h_500mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150904_SEM_RPE1_8h_500mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150902_SEM_chrom_012_500mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150902_SEM_chrom_048_125mM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Waldorf_20150928_SEM_Chromosome_Light.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004915/Gladys_20150908_SEM_RPE1_2h_Exp2.rawprimaryOK200003230matthias.selbach@mdc-berlin.deErik McShaneMass SpectrometryShotgun proteomicsNot availableHumanAneuploidyPre-1http://www.ebi.ac.uk/pride/archive/projects/PXD004915Epithelial CellAHA labelled cells where lyzed and newly synthesized proteins were enriched by click chemistry covalently linking the AHA-bearing proteins to alkyne-agarose beads. After stringent washing proteins where digested "on bead" by Lys-C and trypsin. Peptides where SCX fractionated before being separated on a HPLC system and analyzed on a Q-Exactive mass spectrometer. Denatured and alkylated proteins from standard SILAC experiments were precipitated by Wessel-Flügge precipitation. Protein pellets were re-suspended in Urea buffer and digested by Lys-C and trypsin. Resulting peptides were separated on a 15 cm column packed with C18 material before being analyzed on a Q-Exactive.PrideSILACmonohydroxylated residuedeamidated residueacetylated residueRaw files were analyzed using MaxQuant software version 1.5.1.2. Default settings were kept except that `match between runs` was turned on. Lys8, Arg10, Lys4, Arg6 were set as labels for the AHA p-c data and only Lys8 and Arg10 for the steady state measurements. Oxidation of methionines, n-terminal acetylation and deamidation of aspargine and glutamine residues were defined as variable modifications. Carbamidomethyl of c-termini was set as fixed modification. The in silico digests of the human Uniprot database (2014-10) was done with Trypsin/P. The false discovery rate was set to 1 % and both peptide and protein level.ProteomicsMatthias SelbachQ ExactiveMax Delbrueck centrum, Berlin Charité, BerlinPARTIALHomo Sapiens (human)27720452 McShane E, Sin C, Zauber H, Wells JN, Donnelly N, Wang X, Hou J, Chen W, Storchova Z, Marsh JA, Valleriani A, Selbach M. Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation. Cell. 2016 Oct 20;167(3):803-815.e21 10.1016/j.cell.2016.09.015erik.mcshane@mdc-berlin.deBiologicalMax-Delbrück-Centrum für Molekulare Medizin (MDC)Do young and old protein molecules have the same probability to be degraded? We addressed this question using metabolic pulse-chase labeling and quantitative mass spectrometry to obtain degradation profiles for thousands of proteins. We find that >10% of proteins are degraded non-exponentially. Specifically, proteins are less stable in the first few hours of their life and stabilize with age. Degradation profiles are conserved and similar in two cell types. Many non-exponentially degraded (NED) proteins are subunits of complexes that are produced in super-stoichiometric amounts relative to their exponentially degraded (ED) counterparts. Within complexes, NED proteins have larger interaction interfaces and assemble earlier than ED subunits. Amplifying genes encoding NED proteins increases their initial degradation. Consistently, decay profiles can predict protein level attenuation in aneuploid cells. Together, our data show that non-exponential degradation is common, conserved, and has important consequences for complex formation and regulation of protein abundance.Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation.McShane Erik E, Sin Celine C, Zauber Henrik H, Wells Jonathan N JN, Donnelly Neysan N, Wang Xi X, Hou Jingyi J, Chen Wei W, Storchova Zuzana Z, Marsh Joseph A JA, Valleriani Angelo A, Selbach Matthias MANT-C, anemia hemolytic autoimmune, Click Chemical, Chemical Reaction, Bhlha41, Scx, SCX, Sepharose C1 4B, Antp1, Chemical Technique, bHLHa48, DMANTPE1, Gene, Click Chemical Reaction, Carbamide, protein, Basodexan, Antp P1, Antp P2, Click Chemical Reactions, protein-containing complex, SCXB, body system, SCXA, Buffer, peptide, Karbamid, Polypeptides, Techniques, protein polypeptide chains, peptido, scx, polypeptide chain, idiopathic autoimmune hemolytic anemia, Hu, Harnstoff, familial auto-immune hemolytic anemia (subtype), AntP1, Gene Products, Urea, system, Carmol, protein aggregate, 3.4, Separated, Technique, AIHA, ANTC, Sepharose, antp, Divorced, anatomical systems, peptides, E927b, beta-Trypsin, autoimmune haemolytic anemia, proteins, Divorces, acquired autoimmune hemolytic anemia, xscleraxis, CG1028, HPLC, BG:DS07700.1, C1 4B, column, immuno-hemolytic anemia, Reaction, 6-anhydro-alpha-L-galactopyranosyl-(1->3)-beta-D-galactopyranan, bHLHa41, Click Chemical Technique, D1, Agarose, ANT-P, peptidos, AntP, ANTP, 6-An-alpha-L-Galp-(1->3)-beta-D-Galp-(1->]n, PTHB1, bead, Click, protein complex, 4B, Trypsin/K, Proteins, Chemical Reactions, DmAntp, buffer, Cell, Peptide, ur, Aus, Chemical Techniques, polypeptide, carbamide, native protein, natural protein, carbonyldiamide, C18, [4)-3, BB114693, anemia, Protein, Sepharose C1, Chemistry, familial auto-immune hemolytic anaemia (subtype), autoimmune hemolytic, l(3)84Ba, connected anatomical system, Separation, Ant, Reactions, uree, acquired autoimmune hemolytic anaemia, Separations, urea, Tripcellim, idiopathic autoimmune hemolytic anaemia, autoimmune hemolytic anemia, (1->4)-3, beta Trypsin, UREA, B1., Protein Gene Products, organ system, Click Chemical Techniques, Gene Proteins, Trypure, Click Chemistries, H2NC(O)NH2, autoimmune haemolytic anaemia, Ns, anaemia hemolytic autoimmune, DmelCG1028, Sepharose 4B, Peptid, DRO15DC96Z, Polypeptide, Chemistries, immuno-hemolytic anaemia, AHA, 1728, SclStabilities, multicellular organismal catabolic process, single-organism catabolic process, Stability, breakdown, determination, degradation, chemical analysis, Protein, Protein Stabilities, assay, catabolism., macromolecular stability of proteinBru, IPP2A2, GLUTAMINE, human being, anemia hemolytic autoimmune, Raw, False, 5-diamino-5-oxopentanoic acid, homoaconitase activity, Levoglutamide, protein, Computer, (2S)-2-amino-4-carbamoylbutanoic acid, protein-containing complex, 2-hydroxybutane-1, PHAPII, L-(+)-glutamine, 5730420M11Rik, peptide, Polypeptides, protein polypeptide chains, L-glutamine, L-Glutaminsaeure-5-amid, ClvPrd, peptido, template-activating factor I, polypeptide chain, idiopathic autoimmune hemolytic anemia, familial auto-immune hemolytic anemia (subtype), 2, L-2-aminoglutaramic acid, Del(8)44H, Software Engineering, protein aggregate, Computer Program, Application, AIHA, Svc, Open Source Softwares, SET, proportion, F, peptides, TAF-I, Software Application, (2S)-2, Open, phosphatase 2A inhibitor I2PP2A, ipp2a2, D-Glutamine, Q, autoimmune haemolytic anemia, Open Source Software, Computer Programs and Programming, Acetylations, 2pp2a, proteins, cis-homoaconitase activity, Pyrroline-5-carboxylate reductase-like protein, acquired autoimmune hemolytic anemia, man, CG10574, Computer Software Application, DmelCG4299, set, IGAAD, 2PP2A, Tools, (1R, DmelCG10574, taf-ibeta, immuno-hemolytic anemia, dSET, dSet, L-Glutamine, peptidos, ratio, Applications Software, phapii, HACN activity, Open Source, LysF, 4-tricarboxylate hydro-lyase [(Z)-but-1-ene-1, data, Computer Software, protein complex, acetylation, igaad, StF-IT-1, L-2-Aminoglutaramic acid, Source Softwares, Peptide, Software Tools, Tool, Programs, polypeptide, Program, Computer Applications, Software Tool, P5CR 2, native protein, P5CR 3, natural protein, Lys4, I-2PP2A, P5CR 1, Computer Applications Software, Dm I-2, anemia, I2PP2A, Computer Applications Softwares, Glutamic acid 5-amide, familial auto-immune hemolytic anaemia (subtype), P5C reductase 1, autoimmune hemolytic, Softwares, P5C reductase 3, L-Glutamin, P5C reductase 2, Software, Data Base, L Glutamine, Software Applications, Col4a-1, HLA-DR-associated protein II, DI-2, Glutamic acid amide, Protein., 2S)-1-hydroxybutane-1, Source Software, acquired autoimmune hemolytic anaemia, I-2Dm, 4-tricarboxylate-forming], Engineering, proportionality, idiopathic autoimmune hemolytic anaemia, rate, CG4299, autoimmune hemolytic anemia, inhibitor of granzyme A-activated DNase, (S)-2, human, I-2PP1, L-glutamic acid gamma-amide, Computer Programs, dSET/TAF-Ibeta, Applications, 2610030F17Rik, autoimmune haemolytic anaemia, Applications Softwares, TAF-IBETA, anaemia hemolytic autoimmune, D Glutamine, 1.5.1.2, quotient, Peptid, TAF-Ibeta, Polypeptide, 4-tricarboxylate hydro-lyase activity, immuno-hemolytic anaemia, variable, AA407739, i2pp2a, AHA, Computer Software Applicationsprotein levels, PRE, data, Radiation, human being, anemia hemolytic autoimmune, Cell Lines, high weight, Visible Light, Light, pigmented retinal epithelium, pigmented epithelium, CD258, not genetically inherited, Cell, stratum pigmentosum retinae, Ly113, method, stratum pigmentosum (retina), LIGHT, retinal pigment, idiopathic autoimmune hemolytic anemia, method used in an experiment, hnu, anemia, heavy, familial auto-immune hemolytic anemia (subtype), Line, familial auto-immune hemolytic anaemia (subtype), Cell., stratum pigmentosa retinae, light, autoimmune hemolytic, outer pigmented layer of retina, epithelium, foton, retinal pigment layer, Pulses, AIHA, Lines, Visible Radiations, Radiations, Visible Radiation, RPE, HVEML, HVEM-L, Lichtquant, acquired autoimmune hemolytic anaemia, retinal pigmented epithelium, Photoradiation, autoimmune haemolytic anemia, pigmented retina, idiopathic autoimmune hemolytic anaemia, labeling, autoimmune hemolytic anemia, Visible, acquired autoimmune hemolytic anemia, retinal pigment epithelium, RPE2-1, man, human, LTg, p. pigmentosa retinae, plan specification, azido homoalanine, autoimmune haemolytic anaemia, pigment epithelium of retina, light quantum, Photoradiations, anaemia hemolytic autoimmune, immuno-hemolytic anemia, photon, pigmented retina epithelium, TR2, immuno-hemolytic anaemia, AHA, gammamulticellular organismal catabolic process, single-organism catabolic process, d230, lifespan, Materials, number, Gene, dTAFII250, protein, biosynthesis, Spectrum Analyses, protein-containing complex, EfW1, presence, CG11121, dmTAF[[II]]230, protein polypeptide chains, old, polypeptide chain, DmelCG11121, dmTAF1, Taf230, Mass, Gene Products, Analysis, protein aggregate, Pulses, Mass Spectroscopy, multicellular organismal biosynthetic process, TAF250, Mass Spectrum Analysis, Taf200, single-organism biosynthetic process, somda, dTAF[[II]]250, Genetic, TFIID TAF250, Analyses, cel, formation, catabolism, entire lifespan, cell, entire life cycle, anabolism, Taf1p, proteins, labeling, SO, Probabilities, synthesis, dTAF250, TAF, So, Drl, data, dTAF[[II]]230, TAF[[II]]250, degradation, protein complex, Proteins, TAF200, l(3)84Ab, BG:DS00004.13, TAFII-250, TAF250/230, Cistrons, Spectrum Analysis, Cell, Mdu, dTAF230, Spectroscopy, count in organism, TAFII250, native protein, natural protein, mda, p230, Protein, TAF[[II]]250/230, TFIID, Genetic Materials, ami, Mass Spectrum Analyses, Genetic Material, Taf[[II]]250, Mass Spectrum, breakdown, TAF[[II]]230, Protein., med, life, Spectrometry, common, TAF[II]250, CG17603, TAF[[II]], Protein Gene Products, Gene Proteins, DmelCG17603, Taf250, Material, SR3-5, Cistron, regulation, quantitative, TAF230, presence or absence in organism, TAF1PRE, RPE, anemia hemolytic autoimmune, retinal pigmented epithelium, acquired autoimmune hemolytic anaemia, pigmented retina, autoimmune haemolytic anemia, idiopathic autoimmune hemolytic anaemia, autoimmune hemolytic anemia, pigmented retinal epithelium, pigmented epithelium, retinal pigment epithelium, RPE2-1, acquired autoimmune hemolytic anemia, p. pigmentosa retinae, stratum pigmentosum retinae, stratum pigmentosum (retina), pigment epithelium of retina, autoimmune haemolytic anaemia, anaemia hemolytic autoimmune, retinal pigment, idiopathic autoimmune hemolytic anemia, data., immuno-hemolytic anemia, anemia, familial auto-immune hemolytic anemia (subtype), pigmented retina epithelium, familial auto-immune hemolytic anaemia (subtype), stratum pigmentosa retinae, autoimmune hemolytic, outer pigmented layer of retina, immuno-hemolytic anaemia, epithelium, AHA, retinal pigment layer, AIHA323trueRPE-1 and RPE-1 trisomic AHA p-c and steady state dataWe developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling was also acquired. These datasets contain data for the human RPE-1 and RPE-1 trisomic cell lines.2016-10-062016-09-12PXD00491544664499175582580388043330295358511145120678110806124619624619728421880903206375811363350906836351524260275838538791187947398007NCBITaxon:128057230711143015557146835145262364927467073641135461388580358233888691568895399784475187043511232946928123977133103430436548135833925198183659219813143205965581914936329572637371922226938124781866132838828139558434513586722294914081691804541619341390363453058611204162326442104117061274432108595614963674283166694369455972945281132081003923751463308793694478420896431744712704459741194669400667145458793299352932010198126469041066114575512165994048339251216597216595160621213312831282128033952321832172920435315228112454349825747874980197525555362312951053948824261914548140479NCBITaxon:21575515343989903712059687623193516309800588845581817808173630943179698936160488566140985956641624251505907118702118705320496306386323555432046632226186633129945658606884535121612956717136044100160235870026420369456941016763410210761277351279334281461002935786174633132529111870021576418096997457744491566667547604452467675663366411363367102398709NCBITaxon:4055932582359092788396526498217498216347256569156934982116544872056971089448663661410NCBITaxon:49328715568910245980810005894526808732311561263730391141554219325552187268723872487298727123598755529547624223141463055180066556484198822299767982510216998231174673897641155744689444474153NCBITaxon:5055796016776744685960065349118226370824812991145784586326547876569835566369838192011164034624136810893161001428099612NCBITaxon:548681NCBITaxon:5820546414307747946409860995865228319662776689605960615729513722187594467087562270861711013354833090349741387834306154319474703342499859961588655974649987039202111072878272154078211908570752061894804189853502471587796271902528674636096469644375939146247274612874601134506253452659197221963852638588596NCBITaxon:61575052110517237263419610526419407722774694163481123869NCBITaxon:2703612506174767927459615384005257313136862697969903469417134434636034751536329644042101851097606331492862879669968583558595968912801726071018568729411009118696141633316780781186941436183NCBITaxon:6191118698840231719118691443906NCBITaxon:102982100071257118969419080218787870384766455347095145122578656870861822607046183260705260707NCBITaxon:780NCBITaxon:1502451516658457702921158612184520928574707856100761921263854504457383379192875NCBITaxon:10239NCBITaxon:71428291268063130957044830064110555241590841228233446900815025754122810430085210977970671356225833136185509924NCBITaxon:569382688306405277965833424090615101515NCBITaxon:6312406744824281307670708761383708281677285035158199011951953370249240536088817562615835583337602126719285321773572671193501846451772177437371137121534811215323957676901472664709157125627457903113335508596285963479249130821203511082332648373153214693NCBITaxon:961531858614126220029849376083334111497083332101841385691006581237561275045426662551519788749200375036009412934971530094114903524725175014012573983649908515151438992NCBITaxon:11552374539883747445446415259311792866432359839062887051580119459932716011497861603293338922853422999810894535936457428135122511775058NCBITaxon:1040750277916797181673871349158910306502780291595412629158515849327159570210022612876891355889309451464792031242164133280382803519284343985808946210803481793921182590287889295027906901749342725632273218903829286757221247190400727NCBITaxon:579653745711431123352114020641198108713554771424507229533355571415429315754622421392139011431933829355083329230741129221148489616010677252649997574472758024055071762993665812140535762294128160783957632066724005402965431843183224659620575549922957575759284593307972113549706671436733115955644421432138184922311240607210969762261111795752981818431923347986626528104660104421454747962678253567097477317513565050NCBITaxon:131360769938942468879552014887519795962141421972890922853164351867631854315206494089414424232NCBITaxon:177321409211577892979324470420755910117749906749907312989913112273170187367451011610358132842626587210360983341392696113079813367953588931640710340153033373301581913064139925306883910337212336819940445861153571147161224911131316634305312017554993512993850573176669642688451513458421572678728623862111510416079129831539222746891198351125522856253232136167633631689704132888188551167610807723264248618140843439376520965731116234273068808637721666898626NCBITaxon:2697049339862410312NCBITaxon:1389975864486433197291763438637256737863912334351052311009051414054493179986865540553486545147124546676056810429863571246791884019373345087711982159739757429719133734275779850762554702563188657623951655865886223864946454634076NCBITaxon:1107128835649184588NCBITaxon:59201108743914594326767111167810774084408797789534967944081226900380394351281459536123517861624323039966869597854096409795418694200308954424327786976279129249954511557484090518042643040928692409320030228481231362542642815047510299388653862629034155405120897966287732396692029793628995579315713711700893062186441954866343657558462823823521736231557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