{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Mgf":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004953/Glycine_max_all.mgf.gz"],"Other":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004953/Rawdata_2.tar.gz","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004953/Rawdata_1.tar.gz","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004953/Rawdata_4.tar.gz","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004953/Rawdata_5.tar.gz","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004953/Glycine_max_all.dat","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004953/Rawdata_3.tar.gz","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004953/protein.fa.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":383,"searchCount":0},"additional":{"labhead_mail":["zengweiying_1981@163.com"],"submitter":["weiying zeng"],"technology_type":["Mass Spectrometry","Shotgun proteomics"],"software":[""],"submitter_keywords":["lamprosema indicate (fabricius)","itraq","mrm","Soybean","Soybean; lamprosema indicate (fabricius); itraq; mrm; differential expressed protein","differential expressed protein"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD004953"],"tissue":["Leaf"],"sample_protocol":["1.Protein extracted by TCA method 2.Protein digested by trypsin 3.Peptide labelled by iTRAQ Reagent 8-plex Kit 4.The iTRAQ labeled peptides were fractionated using SCX 5.Each fraction analyzed by LC-MSMS base on AB5600."],"repository":["Pride"],"quantification_method":[""],"modification":[""],"data_protocol":["All mass spectra data were searched by mascot_v2.3.02 against Glycine_max proteome  database."],"omics_type":["Proteomics"],"labhead":["weiying zeng"],"instrument_platform":[""],"labhead_affiliation":["Guangxi Academy of Agricultural Sciences"],"submission_type":["PARTIAL"],"species":["Glycine Max"],"submitter_mail":["zengweiying_1981@163.com"],"publication":["28587595 Zeng W, Sun Z, Cai Z, Chen H, Lai Z, Yang S, Tang X. Proteomic analysis by iTRAQ-MRM of soybean resistance to Lamprosema Indicate. BMC Genomics. 2017 18(1):444 10.1186/s12864-017-3825-0"],"curator_keywords":["Biological"],"submitter_affiliation":["Guangxi Academy of Agricultural Sciences"],"submitter_country":["China"],"pubmed_abstract":["<h4>Background</h4>Lamprosema indicate is a major leaf feeding insect pest to soybean, which has caused serious yield losses in central and southern China. To explore the defense mechanisms of soybean resistance to Lamprosema indicate, a highly resistant line (Gantai-2-2) and a highly susceptible line (Wan 82-178) were exposed to Lamprosema indicate larval feedings for 0 h and 48 h, and the differential proteomic analyses of these two lines were carried out.<h4>Results</h4>The results showed that 31 differentially expressed proteins (DEPs) were identified in the Gantai-2-2 when comparing 48 h feeding with 0 h feeding, and 53 DEPs were identified in the Wan 82-178. 28 DEPs were identified when comparing Gantai-2-2 with Wan 82-178 at 0 h feeding. The bioinformatic analysis results showed that most of the DEPs were associated with ribosome, linoleic acid metabolism, flavonoid biosynthesis, phenylpropanoid biosynthesis, peroxisome, stilbenoid, diarylheptanoid and gingerol biosynthesis, glutathione metabolism, pant hormone signal transduction, and flavone and flavonol biosynthesis, as well as other resistance related metabolic pathways. The MRM analysis showed that the iTRAQ results were reliable.<h4>Conclusions</h4>According to the analysis of the DEPs results, the soybean defended or resisted the Lamprosema indicate damage by the induction of a synthesis of anti-digestive proteins which inhibit the growth and development of insects, reactive oxygen species scavenging, signaling pathways, secondary metabolites synthesis, and so on."],"pubmed_title":["Proteomic analysis by iTRAQ-MRM of soybean resistance to Lamprosema Indicate."],"pubmed_authors":["Zeng Weiying W, Sun Zudong Z, Cai Zhaoyan Z, Chen Huaizhu H, Lai Zhenguang Z, Yang Shouzhen S, Tang Xiangmin X"],"sample_synonyms":["liquid chromatography tandem mass spectroscopy, ANT-C, Procedures, Bhlha41, Scx, SCX, Antp1, SCF receptor activity, bHLHa48, DMANTPE1, Gene, protein, Xkl-1, Antp P1, Antp P2, protein-containing complex, SCXB, SCXA, LC-MS-MS, peptide, Polypeptides, method, Techniques, KL receptor activity, Gsfsco1, peptido, scfr, scx, Method, method used in an experiment, Hu, LC-MSMS, AntP1, Gene Products, Studies, SCO5, SCO1, protein aggregate, Gsfsow3, 3.4, SCFR, Gsfsco5, Technique, SOW3, ANTC, Fdc, antp, LCMSMS, peptides, bases, beta-Trypsin, Basen, W, xscleraxis, CG1028, BG:DS07700.1, Study, Methodological Studies, bHLHa41, Bs, ANT-P, peptidos, AntP, ANTP, Sl, LC-MS2, proto-oncogene c-Kit, Nucleobase, protein complex, Proteins, LC-MS/MS, Tr-kit, Procedure, DmAntp, Peptide, Aus, polypeptide, PBT, LC/MS/MS, native protein, KIT ligand receptor activity, BB114693, Protein, Base2, kl1-A, l(3)84Ba, KIT, XKrk1, tyrosine-protein kinase Kit, pbt, c-KIT, Ant, PRSS, Tripcellim, Methodological, kit, CD117, Methodological Study, beta Trypsin, Protein Gene Products, plan specification, Gene Proteins, Trypure, Base1., c-kit, Ns, DmelCG1028, C-Kit, liquid chromatography-tandem mass spectroscopy, Ssm, Base, xkl-1, liquid chromatography tandem mass spectrometry, Peptid, DRO15DC96Z, Polypeptide, nucleobases, Scl, krk1, base"],"name_synonyms":["Multiple Reaction Monitoring, soybean, Soy Bean, cv. Wye, Soybeans, soybeans., MRM, Soybean, Phaseolus max, Glycine max, proteomic analysis, Bean, Soy Beans, Beans, Soy"],"data_synonyms":["Monoammonium Salt, Cobalt Salt Glycine, Glycine Carbonate (2:1), Glycine Phosphate (1:1), Calcium Salt Glycine, Sodium Hydrogen Carbonate, dmMOF, H2N-CH2-COOH, AW549739, Glycine Phosphate, Monopotassium Salt Glycine, Aminoessigsaeure, Monopotassium, Aminoacetic acid, CG3025, Copper Salt, CG9648, FBN, total expressed protein, Gly, Monosodium Salt Glycine, Monoammonium, Data Base., aminoethanoic acid, Monosodium, Copper Salt Glycine, ACMICD, AI875693, bHLHd7, DmelCG9648, bHLHd6, bHLHd9, bHLHd8, Glycine Carbonate (1:1), DmelCG3025, bHLHd5, bHLHd4, Calcium Salt (2:1), ECTOL1, dMOF, dmax, Hydrochloride, Monoammonium Salt Glycine, MFS1, Glycine Hydrochloride (2:1), WMS, WMS2, C79691, Acid, Aminoacetic Acid, MAX, G, Glycine Hydrochloride, Glycine Sulfate (3:1), Glycine, Hgly, dMax, Monosodium Salt, Monopotassium Salt, MASCOT, Phosphate, MASS, Cobalt Salt, Monolithium Salt, Glyzin, OCTD, Lccp, mKIAA0989, max, MOF, Mof, Salt Glycine, Glycin, Glycocoll, Aminoacetic, Glykokoll, SSKS, AA960152, Leimzucker, 2-aminoacetic acid, GPHYSD2, Proteomes, Calcium Salt, SGS"],"description_synonyms":["DNA (cytosine-5)-methyltransferase 1, Soybeans, insensitive, determination, HSN1E, exits through, Proteins, DNMT, Gene, MCMT, Soy Bean, Mechanisms, Aim, AIM, exact), DNA methyltransferase HsaI, chemical analysis, Protein, susceptible, resistance, Gene Products, CXXC9, study, DNMT1, ADCADN, resistant, UNQ203/PRO229, CT-2, DNMT1_HUMAN, Soybean, Phaseolus max, DNA (cytosine-5-)-methyltransferase 1, Bean, soybeans, Soy Beans, 2-2, exposed, hoja (Spanish, 葉 (Japanese, CXXC-type zinc finger protein 9, Protein Gene Products, soybean, Gene Proteins, cv. Wye, API6, extruding from, Glycine max, Beans, assay, Defense., CXXC finger protein 9, Soy, CLEC2C, RCB1375, m.HsaI, DNA MTase HsaI"],"pubmed_abstract_synonyms":["Metabolic Networks, Networks, black death, Signal Transductions, Acyclic, determination, postnatal development, ribosomal RNA, Linear Diarylheptanoids, growth and development, phenylpropanoid synthesis, 2-phenylchromone, Molecular Biologies, MAGE-E1 antigen, Mechanisms, Receptor Mediated Signal Transduction, flavonoid formation, primary metabolites, exact), Bio Informatics, Stilbenoids, insects, Atelocerata, 2-PHENYL-4H-CHROMEN-4-ONE, Pro Oxidant, Peroxisome, Cell Signaling, peroxisomal, Sinkiang, multicellular organismal biosynthetic process, signal transduction by protein phosphorylation, single-organism biosynthetic process, Biology, Soybean, Hormone Receptor, Stilbene Derivative, anabolism, Soy Beans, Pathways, Bio-Informatic, infection by Yersinia pestis, Computational, 2-Phenyl-4-benzopyron, endocrine, Oxygen, phenylpropanoid biosynthesis, Curcuminoid, HCA1, Bio-Informatics, Derivatives, peroxisome vesicle, Signal Transduction Pathways, Transductions, Computational Molecular Biologies, single organism signaling, 6-gingerol, Soybeans, Diarylheptanoids, Pathway, Oxygen Radical, Biologies, signal transduction by conformational transition, Receptor Agonists, Diaryl Heptanoids, Metabolic Network, Computational Molecular, signal transduction by trans-phosphorylation, flavone, Multiple Reaction Monitoring, Soy Bean, Manchuria, signaling cascade, 3-hydroxy-2-phenylchromone, Computational Molecular Biology, DAMAGE, Hepatocellular carcinoma-associated protein 1, Uniramia, oxidized glutathione reduction, Transduction, Oxygen Radicals, Mainland China, Bioinformatic, Phaseolus max, Cyclic Diarylheptanoids, Radical, System, hexapods, Diaryl, 葉 (Japanese, cv. Wye, Curcuminoids, flavonol, 3-hydroxy-2-phenyl-4H-1-benzopyran-4-one, signaling pathway, metabolites, Linear, pestilential fever, Heptanoids, flavonoid synthesis, 10-gingerol, mMage-e1, Signal Transduction Systems, bioformation, multicellular organismal biosynthetic process., Receptor-Mediated, secondary metabolites, Gene, flavonoid anabolism, Signal Transduction System, Reactive Oxygen Intermediates, Network, Hormone, pest, Signal Transduction, Diarylheptanoid Derivatives, 2-Phenyl-4-chromone, Flavon, 14C-labeled, Gene Products, Agonists, 2-phenyl-4H-benzopyran-4-one, Reactive, Stilbene, hormones, MRM, soybeans, Signal, Hormone Receptor Agonists, 2-2, Signal Pathways, AI847422, Signal Transduction Pathway, Oxygen Species, soybean, Diarylheptanones, Acyclic Diarylheptanoids, signalling pathway, Active Oxygen Species, Defense, Beans, signal transduction by cis-phosphorylation, Pro-Oxidant, Cyclic Diarylheptanoid, Active Oxygen, flavonoid biosynthesis, Signal Pathway, Soy, Molecular Biology, Pro Oxidants, synthesize, Stilbenoid, Proteins, Alpha-dystrobrevin-associated MAGE Protein, 2-phenyl-gamma-benzopyrone, Receptor-Mediated Signal Transductions, People's Republic of China, Bioinformatics, development, linoleic acid metabolism, Stilbene Derivatives, Inner Mongolia, Receptor-Mediated Signal Transduction, Protein, chemical analysis, Systems, Active, Insects, Derivative, signalling cascade, membrane bound ribosome, Diarylheptanoid, Metabolic Pathways, free ribosome, 2-Phenylbenzopyran-4-one, Ribosome, 2-phenyl-4H-1-benzopyran-4-one, Metabolic, RGD1560259, Bean, Diarylheptanoid Derivative, postnatal growth, metabolite, hoja (Spanish, Insect, Protein Gene Products, Gene Proteins, phenylpropanoid formation, Development and Growth, signalling process, Glycine max, Pro-Oxidants, Metabolic Pathway, phenylpropanoid anabolism, assay, Cyclic, (6)-gingerol, growth, RCB1375, Tracheata"],"pubmed_title_synonyms":["Multiple Reaction Monitoring, soybean, Soy Bean, cv. Wye, Soybeans, soybeans., MRM, Soybean, Phaseolus max, Glycine max, proteomic analysis, Bean, Soy Beans, Beans, Soy"],"view_count":["383"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic Analysis by iTRAQ-MRM of Soybean Resistance to Lamprosema Indicate","description":"This study was conducted to: (1)comparative analysis of expression profiles of proteins in soybean leaf(a highly resistant line (Gantai-2-2) and a highly susceptible line (Wan 82-178) were exposed to Lamprosema indicate larval feedings for 0 h and 48 h);(2)explore how these proteins participated in the responses to resistance;(3) aim at resolving the soybeans’ constitutive and inducible defense mechanisms in response to Lamprosema indicate at the proteomic level.","dates":{"publication":"2018-10-26","submission":"2016-09-13"},"accession":"PXD004953","cross_references":{"TAXONOMY":["NEWT:6945","NEWT:3555","NEWT:2","NEWT:157546","NEWT:35554","NEWT:38942","NEWT:307972","NEWT:32046","NEWT:544496","NEWT:2042546","NEWT:43179","NEWT:4513","NEWT:376741","NEWT:55153","NCBITaxon:10407","NEWT:1736309","NEWT:1211601","NEWT:876138","NEWT:237561","NEWT:6928","NEWT:10036","NEWT:36745","NEWT:1351","NEWT:1438992","NEWT:2649997","NEWT:272563","NEWT:224326","NCBITaxon:79857","NEWT:95648","NEWT:3885","NEWT:3888","NEWT:1589","NEWT:135622","NEWT:6915","NEWT:3649","NEWT:101510","NEWT:3880","NEWT:272559","NEWT:3641","NEWT:383379","NEWT:10029","NEWT:1000589","NEWT:85962","NEWT:317447","NEWT:7955","NEWT:7959","NEWT:2261","NEWT:4565","NEWT:1264690","NEWT:515619","NEWT:192875","NEWT:34305","NEWT:59729","NCBITaxon:183674","NEWT:224308","NEWT:626528","NEWT:139927","NEWT:4558","NEWT:209285","NEWT:1283","NEWT:931281","NEWT:4550","NEWT:1000561","NCBITaxon:79824","NEWT:4787","NCBITaxon:4563","NEWT:5755","NEWT:3218","NEWT:5759","NEWT:1736231","NEWT:1270","NEWT:2242","NEWT:4784","NEWT:11320","NEWT:360106","NEWT:287","NEWT:10117","NEWT:10239","NEWT:10116","NEWT:1280","NEWT:1735272","NEWT:83334","NEWT:83332","NEWT:44685","NEWT:1148","NEWT:580240","NEWT:11676","NEWT:55571","NEWT:100226","NEWT:4530","NEWT:4896","NEWT:75058","NEWT:13616","NEWT:1094343","NEWT:296543","NEWT:1773","NEWT:1895","NEWT:1182590","NEWT:935293","NEWT:64152","NEWT:4924","NEWT:749200","NEWT:990346","NEWT:145953","NEWT:257309","NEWT:100816","NEWT:263","NEWT:230741","NEWT:284812","NCBITaxon:1313","NEWT:43330","NEWT:44544","NEWT:4911","NEWT:645463","NEWT:3702","NEWT:129249","NEWT:990119","NEWT:408172","NEWT:408170","NEWT:493760","NEWT:260710","NEWT:257313","NEWT:400772","NEWT:3708","NEWT:128161","NEWT:332648","NEWT:106592","NEWT:536231","NEWT:1432138","NEWT:10312","NEWT:1424507","NCBITaxon:1773","NEWT:9598","NEWT:8030","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:480","NEWT:4909","NEWT:67767","NEWT:46835","NEWT:376686","NEWT:95486","NEWT:9103","NEWT:10306","NCBITaxon:2759","NEWT:8022","NEWT:145943","NCBITaxon:4932","NEWT:595536","NEWT:240906","NEWT:593117","NEWT:3635","NEWT:5811","NEWT:235443","NEWT:411483","NEWT:884019","NEWT:198215","NEWT:411490","NEWT:983964","NEWT:169963","NEWT:499175","NEWT:476272","NEWT:367830","NEWT:178616","NEWT:410289","NEWT:373153","NEWT:360094","NEWT:470","NEWT:1313","NEWT:411469","NEWT:84023","NCBITaxon:5811","NEWT:411464","NEWT:411460","NEWT:2762","NEWT:1174673","NEWT:562","NEWT:411470","NEWT:33952","NEWT:2094720","NCBITaxon:2697049","NEWT:28038","NEWT:1423","NEWT:4932","NEWT:3603","NEWT:2759","NEWT:3847","NEWT:327159","NEWT:178876","NEWT:327160","NEWT:573","NEWT:9031","NEWT:7091","NEWT:241368","NEWT:42528","NEWT:190802","NEWT:9778","NEWT:150475","NEWT:9417","NEWT:7111","NEWT:347515","NEWT:1216979","NEWT:5180","NEWT:256737","NEWT:115104","NEWT:1081927","NEWT:1238993","NEWT:67825","NEWT:185579","NEWT:13076","NEWT:1249668","NEWT:317","NEWT:7227","NEWT:7469","NEWT:885318","NEWT:9402","NEWT:415540","NEWT:4081","NEWT:554","NEWT:98334","NEWT:426428","NEWT:7574","NEWT:7215","NEWT:575412","NEWT:29204","NEWT:2172103","NEWT:507601","NCBITaxon:6157","NEWT:746360","NEWT:6239","NEWT:470150","NEWT:216257","NEWT:9986","NEWT:4054","NEWT:226186","NEWT:1268063","NEWT:8782","NEWT:1263854","NEWT:435590","NEWT:1902","NEWT:160488","NEWT:28104","NCBITaxon:2","NEWT:985076","NEWT:1215323","NEWT:6192","NEWT:28532","NCBITaxon:38727","NEWT:2829","NEWT:216599","NEWT:216595","NEWT:243230","NEWT:8355","NEWT:9685","NEWT:7029","NEWT:1283300","NEWT:6183","NEWT:6063","NEWT:334747","NEWT:61235","NEWT:6289","NEWT:436486","NEWT:6287","NEWT:300641","NEWT:727","NEWT:9796","NEWT:725","NEWT:170187","NEWT:260707","NCBITaxon:6191","NEWT:1836","NEWT:185431","NEWT:29760","NEWT:260704","NEWT:703612","NEWT:260705","NEWT:80863","NEWT:2697049","NCBITaxon:6073","NEWT:884204","NEWT:6279","NEWT:1123869","NEWT:9544","NEWT:7370","NEWT:83906","NEWT:607699","NEWT:6282","NEWT:208964","NEWT:1134506","NEWT:575584","NEWT:38783","NEWT:8727","NEWT:4006","NEWT:8726","NEWT:6426","NEWT:6669","NEWT:10090","NEWT:4120","NEWT:51515","NEWT:5693","NEWT:8724","NEWT:51511","NEWT:92867","NEWT:8723","NEWT:5334","NCBITaxon:10359","NEWT:242619","NEWT:632957","NEWT:373995","NEWT:5689","NEWT:544404","NEWT:9925","NEWT:8839","NEWT:4232","NEWT:2758385","NEWT:4113","NEWT:837","NEWT:11298","NEWT:171101","NEWT:714","NEWT:421932","NEWT:196627","NEWT:5691","NEWT:627025","NEWT:1097677","NEWT:61674","NEWT:1117957","NEWT:9913","NEWT:4100","NEWT:1076","NEWT:6763","NEWT:803","NEWT:29722","NEWT:380394","NEWT:1692259","NEWT:180066","NEWT:135588","NEWT:1843183","NEWT:58002","NEWT:4577","NEWT:1416333","NEWT:5664","NEWT:2157","NEWT:418985","NEWT:146479","NEWT:1911079","NEWT:1480154","NEWT:1274414","NEWT:27606","NEWT:59202","NEWT:9975","NEWT:9612","NEWT:38865","NEWT:51953","NEWT:3197","NEWT:9615","NEWT:10299","NEWT:860688","NEWT:36329","NEWT:1147787","NCBITaxon:3044782","NEWT:72407","NEWT:349741","N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