Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT3BME4_200ngul_4uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT2BME4_200ngul_4uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT3TAT2_200ngul_10uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT2BME1_200ngul_2p9uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT1BME4_200ngul_1p8uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT1TAT3_200ngul_1p8uLinjectin.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT1TAT1_200ngul_1p9uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT1BME2_200ngul_2p2uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT3BME2_200ngul_2uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT1BME3_200ngul_3p4uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT2TAT4_200ngul_2p1uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT1TAT2_200ngul_4p2uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT2TAT1_200ngul_2p4uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT1BME1_200ngul_1p8uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT3BME1_200ngul_1p2uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT3TAT1_200ngul_3uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT3BME3_200ngul_1p8uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT2BME2_200ngul_2p9uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT3TAT3_200ngul_10uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/150430_Kim_DionexQE_HT2TAT3_200ngul_1p7uLinjection.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006314/txt.zipprimaryOK200002540jonathan.blackburn@uct.ac.zaKim GurwitzMass SpectrometryShotgun proteomicsHuman Immunodeficiency Virus Infectious DiseaseNot availableLcmsmsHiv associated neurocognitive disordersHuman neuronal cellsHiv-tathttp://www.ebi.ac.uk/pride/archive/projects/PXD006314NeuronCell CultureNeural Stem CellEmbryonic Stem CellCells were lifted using tryp-LE (Gibco), washed with phosphate buffered saline (PBS) and pelleted at 600g. Supernatants were aspirated and cell pellets flash frozen in liquid nitrogen. Cell pellets were lysed in the presence of 1x protease inhibitors (Sigma Aldrich) and 1x phosphatase inhibitors (PhosStop, Roche) using a 1x Tris/ HCl pH7.6 radioimmunoprecipitation assay (RIPA) buffer excluding dithiothreitol (DTT), which otherwise interferes with bicinchoninic acid (BCA) protein quantitation. Cell lysates were incubated at 4°C for 1 hour with 1μl Benzonase nuclease ultrapure (Sigma Aldrich). Subsequently, lysates were centrifuged at 14 000g for 10 minutes and supernatants containing the cell protein content were transferred to new 1.5ml plastic tubes. Protein quantitation was then performed using the Pierce BCA Protein Assay Kit microplate procedure (ThermoScientific) with BSA as the standard. Tryptic peptides for MS analysis were generated on 30kDa molecular weight cutoff (MWCO) filters (Millipore) using the Filter-Aided Sample Preparation (FASP) protocol (Wiśniewski et al. 2009). Samples were desalted using reverse-phase C18 chromatography (C18 columns manufactured in-house from C18 discs, Millipore) prior to MS analysis. Briefly, C18 columns were equilibrated with 80% mass spectrometry (MS) grade acetonitrile (ACN, Sigma Aldrich) acidified with 0.1% formic acid (FA). 10μg peptide from each sample was loaded on separate C18 columns - one column per sample – and desalted by the addition of 2% ACN acidified with 0.1% FA. Finally, peptides were eluted in to glass vials with 60% ACN acidified with 0.1 % FA. Desalted peptides were dried at room temperature using a SpeedyVac (Savant) and resuspended in 0.1% FA (Sigma Aldrich) in ultrapure dH2O to a final concentration of 250ng/μl, which was estimated from BCA quantitation at the protein level on the assumption of no protein loss during FASP. Liquid chromatography tandem MS (LC-MS2) analysis was performed on a Q Exactive MS instrument coupled to a Dionex Ultimate 3500 RSLCnano ultra high-pressure nano liquid chromatography system (both Thermo Fischer Scientific). All samples were injected into the sample loop at a flow rate of 5μl/min in 2% buffer B (0.1%FA/ACN) and 98% buffer A (0.1% FA/ultrapure dH2O). Samples were then loaded onto a 40cm, 75μm diameter, C18 reverse phase analytical column (3.6μm diameter Aeris Peptide C18 packing material, packed in house; Phenomenex 04A-4507) and subsequently eluted during a 120-minute segmented gradient (4-30% buffer B) at a flow rate of 400nl/min. At the end of each sample run, buffer B was ramped to 80% for 10min, which was then followed by a 20-minute wash cycle, from 2% to 50%, and a 20-minute column equilibration. After every fourth sample, a longer wash (80 minutes) was run. Mass spectra were collected with settings essentially as described by Nel et al. (2015) with the following amendments: intensity threshold for ion selection was set at 6.3e3; isolation window for tandem mass spectrometry (MS2) was set at 2.0 m/z; and MS2 spectra were acquired at a target value of 5e4.PrideLabel freeNo PTMs are included in the datasetRaw mass spectra were processed for peptide peak identification and quantitation by MaxQuant software (version 1.5.03) (Cox et al. 2009). Default MaxQuant settings were used with the following modifications: labeling was set to singlet, label free quantitation (LFQ) was selected for relative label free quantitation, ‘match between runs’ was selected, and only peptides unique to a protein group were used for quantitation. MaxQuant’s inbuilt search engine, Andromeda (Cox et al. 2011), was used for protein group identification using the UniProt human proteome database as the reference (FASTA, downloaded on 12/01/15, containing 121996 sequence entries). The datasets generated by MaxQuant was filtered to exclude protein groups that mapped to a sequence in the decoy database or that mapped to a known contaminant. In addition, only protein groups that had an LFQ measurement in each experimental sample, and had a satisfactory posterior error probability (PEP) score (< 0.01), were taken forward for downstream data analyses.ProteomicsProf. Jonathan BlackburnQ ExactiveDepartment of Integrative Biomedical Science, Blackburn Group, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South AfricaPARTIALHomo Sapiens (human)28611588 Gurwitz KT, Burman RJ, Murugan BD, Garnett S, Ganief T, Soares NC, Raimondo JV, Blackburn JM. Time-Dependent, HIV-Tat-Induced Perturbation of Human Neurons In Vitro: Towards a Model for the Molecular Pathology of HIV-Associated Neurocognitive Disorders. Front Mol Neurosci. 2017 May 29;10:163. eCollection 2017 10.3389/fnmol.2017.00163kgurwitz@gmail.comBiomedicalUniversity of Cape TownA significant proportion of human immunodeficiency virus type 1 (HIV)-positive individuals are affected by the cognitive, motor and behavioral dysfunction that characterizes HIV-associated neurocognitive disorders (HAND). While the molecular etiology of HAND remains largely uncharacterized, HIV transactivator of transcription (HIV-Tat) is thought to be an important etiological cause. Here we have used mass spectrometry (MS)-based discovery proteomics to identify the quantitative, cell-wide changes that occur when non-transformed, differentiated human neurons are treated with HIV-Tat over time. We identified over 4000 protein groups (false discovery rate <0.01) in this system with 131, 118 and 45 protein groups differentially expressed at 6, 24 and 48 h post treatment, respectively. Alterations in the expression of proteins involved in gene expression and cytoskeletal maintenance were particularly evident. In tandem with proteomic evidence of cytoskeletal dysregulation we observed HIV-Tat induced functional alterations, including a reduction of neuronal intrinsic excitability as assessed by patch-clamp electrophysiology. Our findings may be relevant for understanding <i>in vivo</i> molecular mechanisms in HAND.Time-Dependent, HIV-Tat-Induced Perturbation of Human Neurons <i>In Vitro</i>: Towards a Model for the Molecular Pathology of HIV-Associated Neurocognitive Disorders.Gurwitz Kim T KT, Burman Richard J RJ, Murugan Brandon D BD, Garnett Shaun S, Ganief Tariq T, Soares Nelson C NC, Raimondo Joseph V JV, Blackburn Jonathan M JMsodium salt, TAFII30, ammonium formate, 13C-labeled, MeCN, cadmium salt, Molecular Weights, ion, Tandem, fond, magnesium formate, monosomy 2q32, CycEI, zinc salt, Bca, 5730420M11Rik, dmTAF[[II]]230, Polypeptides, protein polypeptide chains, KL receptor activity, M(3L)i, hydrogen chloride, AU041226, cobalt(II) formate dihydrate, Protease Inhibitor, FLASH, 30kDa, CH3-C#N, B1, SCO5, HCL-C, 1, 2, AGM4, SCO1, Analysis, Gsfsow3, WMS, (R*, bca, SET, C79325, TFIID TAF250, sarcoma of breast, Analyses, cel, Molecular, Radioimmunoprecipitation Analyses, M, DmelCG13176, N, Assays, phosphatase 2A inhibitor I2PP2A, Weights, ORF19, composition, proteins, W, RIP25, SURGICAL AND MEDICAL PROCEDURES, Acdc, AI047805, DmelCG4299, isolation and purification, set, Wasserstoffchlorid, Peptidohydrolase Inhibitor, h, column, ACN, D1, Acn, sample, n, Bs, monosomy 2q32-q33, D9, phosphatase, Farr, M(3)RpS17, nickel salt, SGS, LC-MS2, dTAF[[II]]230, BZRP, cobalt (+2) salt, Liquid Chromatography, TAF200, Procedure, breast sarcoma, Cyc E, Spectrum Analysis, not genetically inherited, PBS, adipo, ACMICD, PBR, PBT, sodium (4:1:1) salt, BG:DS07108.3, Stickstoff, magnesium salt, Dm1, M(3)i(55), Weight, SATB2-associated syndrome, Hydrolase Inhibitor, Chlorwasserstoff, Del(2)(q32), 1110049F14Rik, leukemic reticuloendotheliosis, l(2)05206, END, rpS17, pbt, 2q32q33 microdeletion syndrome, HLA-DR-associated protein II, Antagonists, instrument, DI-2, LY57, content, Acrp30, I-2Dm, formate, Spectrometry, cromium (+3), inhibitor of granzyme A-activated DNase, study protocol, I-2PP1, Speed, nitrogen, Painful bladder syndrome, TAF-IBETA, Taf250, liquid chromatography-tandem mass spectroscopy, ammonium (4:1) salt, TAF-Ibeta, Endopeptidase, Peptide Peptidohydrolase Inhibitor, methyl cyanide, chlorane, lead (+2) salt, TAF230, krk1, BPBS, Cleland Reagent, nickel formate dihydrate, cycline, lead formate, sarcoma of the breast, Endopeptidase Inhibitors, chlorure d'hydrogene, DmcyclinE, aluminum salt, number, Pierce, 4-dimercapto-, R*)-, protein-containing complex, thomson, Chalk, body system, CG7826, mass-to-charge ratio, cdi7, SATB2 syndrome, M(3)i, cyclinE, Radioimmunoprecipitation, Radioimmunoprecipitation Assays, isolation, Ly57, LC-MSMS, CG7835, Cdi7, CDI7, CG42273, system, 5E4., Peptide Hydrolase, anatomical systems, dTAF[[II]]250, FAM39E, Acinus, Hydrogen chloride, interventionDescription, thallium (+1) salt, cell, rubidium salt, 2pp2a, pk18, methanoic acid, l(3)dtOA4, DmcycE, ions, Antagonist, MBR, CG10574, CD156, acinusL, dTAF250, hairy cell leukemia, 2PP2A, Protease Antagonists, acinusS, M(3)i[55], dSET, dSet, PTBR, Peptide Peptidohydrolase Inhibitors, peptidos, Chromatographies, PTHB1, proto-oncogene c-Kit, 1-(14)C-labeled, Assay, Clelands Reagent, BG:DS00004.13, chloridohydrogen, potassium formate, copper (+2) salt, compositionality, C79664, acetonitrile, buffer, Cell, mKIAA0670, dTAF230, polypeptide, Wash2, Wash1, l(2)k02514, native protein, Temperatures, DmCycE, I-2PP2A, KIT ligand receptor activity, C18, cupric formate, chemical analysis, Dm I-2, TAF[[II]]250/230, BcDNA:RE44119, MFS1, aluminum formate, Mass Spectrum Analyses, XKrk1, Cleland's, Intervention, Taf[[II]]250, AU020952, lithium formate, l(2)k02602, Radioimmunoprecipitation Analysis, M(3)q, azote, ethanenitrile, Mass Spectrometry-Mass Spectrometry, Peptidohydrolase Inhibitors, CD117, mDRC, CD156a, anon-EST:Posey48, apM1, ME-IV, plan specification, Cleland's Reagent, D-CycE, Peptidase, Antiprotease, C-Kit, Ssm, structure, xkl-1, M(3L)i[55], liquid chromatography tandem mass spectrometry, Peptide Hydrolase Inhibitors, phosphates, quantitative, hydrochloric acid, APN, liquid chromatography tandem mass spectroscopy, Rps17, IPP2A2, Endopeptidase Inhibitor, Meth, NCMe, determination, selection process, instrument configuration, classic hairy cell leukaemia, Peptide Hydrolase Inhibitor, zinc formate, RPS17, lead salt, Hydrogenchlorid, protein, Xkl-1, Pi, composed of, Mass Spectrometry Mass Spectrometry, peptide, M(3)67, Gsfsco1, ammonium tetraformate, peptido, ClvPrd, Ccne, BANF, Inhibitors, Min, Hydrolase Inhibitors, protein aggregate, monosomy 2q32q33, Gsfsco5, DmelCG42273, SOW3, Mass Spectrum Analysis, LCMSMS, peptides, TAF-I, 2610036I19Rik, l(3)67BDo, 2610510L13Rik, E430039A18Rik, min, mAPC, IBP, purification, Mass Spectrometry, copper, Estimated, IGAAD, fSAP152, lithium salt, chromosome 2q32-q33 deletion syndrome, mKIAA1315, Sputolysin, DmelCG10574, Ice, Lyw-57, Bladder pain syndrome, Sl, GPHYSD2, ammonium (2:1) salt, phapii, PLATEST, Peptidase Inhibitor, LC-MS/MS, Dmel_CG7826, M(3)67C, StF-IT-1, Tr-kit, Th, TAFII-250, CED-4, classic hairy cell leukemia, TAF250/230, Peptide Peptidohydrolase, br37, Spectroscopy, glass, SLP65, Ly-57, TAFII250, AA387232, Cleland, Ionen, HCL, kl1-A, KIT, WASH, nickel (+2) salt, Dmel_CG7835, Crystal, Mnb, MNB, tyrosine-protein kinase Kit, SLP-65, ammonium salt, Painful Bladder Syndrome, BASH, MS/MS, Xylella fastidiosa str. Temecula1, HCl, Rp S17, cobaltous formate, Del(2)(q32q33), 3-Butanediol, kit, MASS, CG4299, AW124434, CG17603, TAF[[II]], organ system, Peptidase Inhibitors, l35Dd, phosphate, phosphate ions, SR3-5, copper salt, Antiproteases, Polypeptide, 7N, Inhibitor, i2pp2a, Platelets, Bladder Pain Syndrome, d230, Reagent, cesium salt, SCF receptor activity, FBN, D4Ertd659e, dTAFII250, Protease, cycE, Spectrum Analyses, ACINUS, EfW1, presence, l(2)br37, PHAPII, LC-MS-MS, CYCLE, Buffer, Intervention or Procedure, formic acid, method, potassium salt, M(3)S33, polypeptide chain, scfr, template-activating factor I, dmTAF1, glass syndrome, Taf230, ECTOL1, 14C-labeled, p63, method used in an experiment, p65, Tina, Mass, Protease Antagonist, SCFR, cloruro de hidrogeno, Mass Spectroscopy, TAF250, Taf200, Fdc, Glass, CYCE, Plastic, DmelCG3938, iones, MS2, CyclE, ipp2a2, 3938, Interventional, Minute, Taf1p, calcium formate, Farr Assay, Xylella fastidiosa (strain Temecula1 / ATCC 700964), OCTD, l(2)k05007, Taf2h, dm-cycE, DmelCG3922, microplate, taf-ibeta, liquid, cromium (+3) salt, TAF, sodium formate, HHT1, BLNK-S, Intervention Strategies, 2q32-q33 microdeletion syndrome, DYRK1, TAF[[II]]250, nickel formate, DBI, 3H-labeled, 2q32q33 microdeletion syndromes, protein complex, igaad, strontium formate, l(3)84Ab, strontium salt, Peptide, [HCl], count in organism, LC/MS/MS, PKBS, natural protein, p230, Protein, I2PP2A, Hydrochloride, TFIID, Dyrk1, CyeE, connected anatomical system, ACETONITRILE, tandem MS, cyanomethane, WMS2, CG13176, Mass Spectrum, c-KIT, SAS, 10^[-9], TAF[[II]]230, CC1, l(2)35Dd, phosphoric monoester hydrolase activity, TAF[II]250, GBP28, chromic formate, sample population, nitrogeno, CG3922, dSET/TAF-Ibeta, c-kit, 2610030F17Rik, DmelCG17603, Pressures, Ion, concentration, SSKS, calcium salt, Peptid, assay, ORW1, SCH, AA407739, CG3938, TAF1, presence or absence in organismOrganic Mental Disorders, Delirium, Mild Neurocognitive Disorders, Human T-Cell Lymphotropic Virus Type III, Human Immunodeficiency Virus, Alpha-TAT, Traumatic Psychoses, TAT, c6orf134, Human T Lymphotropic Virus Type III, human being, Disorders, Neurocognitive Disorders, Effects, Alpha-TAT1, Nerve, Mild, Amnestic, Organic Mental, Longterm Effect, AIDS Virus, HTLV-III, Psychoses, Neurocognitive Disorder, Organic Mental Disorder, Cell, Long Term, Human Immunodeficiency, mec17, Human, period, Lymphadenopathy Associated Virus, Traumatic, Organic, Mild Neurocognitive Disorder, AIDS, Immunodeficiency Virus, Long Term Effects, Virus, 2610110G12Rik, Human T-Cell Leukemia Virus Type III, Effect, AIDS virus, 0610011P08Rik, Dementia, 3110080J08Rik, Acquired Immune Deficiency Syndrome Virus, Human T-Lymphotropic Virus Type III, Nerve Cells, Lymphadenopathy-Associated, Viruses, AIDS Viruses, Longterm, MEC17, Mild Neurocognitive, Neurocognitive., Immunodeficiency Viruses, Cognitive Disorders, Long-Term, Clerambault Syndrome, Human T Cell Leukemia Virus Type III, man, LAV-HTLV-III, human, Longterm Effects, Acetyltransferase mec-17 homolog, Psychotic, Nbla00487, Kandinsky Syndrome, Neurocognitive, Disorder, Acquired Immunodeficiency Syndrome Virus, Cells, Organic Brain Syndrome, Lymphadenopathy-Associated Viruses, Nonpsychotic, Neuron, Long-Term Effect, HIV, 2610008K08Rik, Mental Disorders, associated, Human Immunodeficiency Viruses, Nerve Cell, Nonpsychotic Organic Brain Syndrome, time, Long-Term Effects, Human T Cell Lymphotropic Virus Type III, C6orf134, Mec17, 2.3.1.108, Mental Disorder, Lymphadenopathy-Associated Virus70zpep, Bru, IPP2A2, PEP/X4, cox4, PP14, Raw, DmelCG30170, LYP2, b(2)gcn, LYP1, protein, DmelCG6143, 5730420M11Rik, peptide, Polypeptides, protein polypeptide chains, ClvPrd, peptido, posterior, Pop, Software Engineering, Analysis, DmelCG10664, protein aggregate, WMS, Computer Program, Svc, LyP, SET, peptides, Analyses, TAF-I, COXIV, Open, phosphatase 2A inhibitor I2PP2A, Computer Programs and Programming, X4/PEP, proteins, PEPPER, COXA_DROME, free, DmelCG4299, LYP, set, IGAAD, Lymphoid phosphatase, Cox4, DmelCG10574, PrEP, sample, 1.3.3.3, PAEG, F12B17_170, GPHYSD2, COX-VA, fs(2)eo-C, posterior end of organism, SGS, phapii, CG6143, T5E21.12, StF-IT-1, ben(2)gcn, Search, PEPINO, Source Softwares, Software Tools, ACMICD, Programs, Program, Computer Applications, VA, AL024441, Computer Applications Software, 3.1.3.48, Computer Applications Softwares, Softwares, COX IV, T5E21_12, PEST-domain phosphatase, Software Applications, bcgn, HLA-DR-associated protein II, F20B18.110, DI-2, Va, Coprogen oxidase, Source Software, I-2Dm, COX IV-1, F20B18_110, AI047692, MASS, CG4299, inhibitor of granzyme A-activated DNase, human, GD, experimental procedures, I-2PP1, Applications, GdA, TAF-IBETA, GdF, PNBP, TAF-Ibeta, Polypeptide, DmelCG14724, GdS, i2pp2a, PEP, Proteomes, cg10331, Cox4a, Gruppe, Computer Software Applications, human being, experimental, FBN, gcn, Computer, protein-containing complex, pep, PHAPII, fs(2)eo6, CG10664, CG30170, template-activating factor I, polypeptide chain, ECTOL1, IV, Del(8)44H, Application, Open Source Softwares, Search Engines, Coproporphyrinogenase, methods, Software Application, experimental section, ipp2a2, Open Source Software, 2pp2a, labeling, man, CG10331, CG10574, Probabilities, Computer Software Application, CG10330, OCTD, 2PP2A, Tools, taf-ibeta, grupos, PASTICCINO 2, COX, BGCN, dSET, dSet, peptidos, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, Applications Software, Open Source, Computer Software, grupo, protein complex, Bgcn, igaad, D10Wsu136e, total expressed protein, b(2)cgn, Peptide, Engine, Tool, group, polypeptide, Software Tool, native protein, natural protein, I-2PP2A, Dm I-2, Protein, I2PP2A, sequence, p100, MFS1, IV-1, Data Analyses., Software, WMS2, Data Base, F12B17.170, Col4a-1, Ptpn8, Engineering, tend, Rest, fs(2)eoQS2, primary structure of sequence macromolecule, sample population, Computer Programs, dSET/TAF-Ibeta, 2610030F17Rik, CG14724, Applications Softwares, Data, SSKS, Peptid, PE, AI450383, PTPN8, AA407739, groupeliquid chromatography tandem mass spectroscopy, Human T-Cell Lymphotropic Virus Type III, d230, c6orf134, human being, Peptidomics, Effects, Alpha-TAT1, Nerve, number, AIDS Virus, HTLV-III, dTAFII250, broad, EfW1, presence, Human Immunodeficiency, mec17, Long Term, LC-MS-MS, Human, dmTAF[[II]]230, Lymphadenopathy Associated Virus, period, dmTAF1, Taf230, LC-MSMS, Virus, Human T-Cell Leukemia Virus Type III, Effect, TAF250, 0610011P08Rik, 3110080J08Rik, Taf200, Human T-Lymphotropic Virus Type III, LCMSMS, dTAF[[II]]250, TFIID TAF250, Viruses, cel, Longterm, cell, MEC17, Immunodeficiency Viruses, Taf1p, Long-Term, man, Human T Cell Leukemia Virus Type III, dTAF250, Acetyltransferase mec-17 homolog, Lymphadenopathy-Associated Viruses, Neuron, HIV, Long-Term Effect, 2610008K08Rik, TAF, Human Immunodeficiency Viruses, Nerve Cell, Long-Term Effects, Mec17, 2.3.1.108, Lymphadenopathy-Associated Virus, LC-MS2, Human Immunodeficiency Virus, dTAF[[II]]230, TAF[[II]]250, Alpha-TAT, TAT, Human T Lymphotropic Virus Type III, wide/broad, HAND., LC-MS/MS, TAF200, Longterm Effect, l(3)84Ab, BG:DS00004.13, TAFII-250, TAF250/230, Cell, dTAF230, count in organism, LC/MS/MS, TAFII250, AIDS, Immunodeficiency Virus, p230, Long Term Effects, TAF[[II]]250/230, TFIID, 2610110G12Rik, AIDS virus, Taf[[II]]250, Acquired Immune Deficiency Syndrome Virus, TAF[[II]]230, Nerve Cells, Lymphadenopathy-Associated, AIDS Viruses, TAF[II]250, CG17603, TAF[[II]], human, LAV-HTLV-III, Longterm Effects, wide, Nbla00487, DmelCG17603, Taf250, liquid chromatography-tandem mass spectroscopy, SR3-5, Cells, Acquired Immunodeficiency Syndrome Virus, liquid chromatography tandem mass spectrometry, quantitative, time, Human T Cell Lymphotropic Virus Type III, C6orf134, TAF230, presence or absence in organism, TAF1Organic Mental Disorders, Human T-Cell Lymphotropic Virus Type III, c6orf134, Disorders, Mild, AIDS Virus, protein, spef1a, Human Immunodeficiency, Long Term, Lymphadenopathy Associated Virus, dysfunctional, dmTAF[[II]]230, protein polypeptide chains, Readability, forelimb autopod, Virus, symptoms, pathogenesis, Analysis, protein aggregate, Effect, Human immunodeficiency virus, 3110080J08Rik, Mass Spectrum Analysis, SPEF1A, Gene Expressions, TFIID TAF250, Analyses, cel, HAND, Mild Neurocognitive, Cognitive Disorders, bHLHa28, proteins, hand, Acetyltransferase mec-17 homolog, DNA-dependent, Kandinsky Syndrome, C20orf28, Mental Disorders, 2610008K08Rik, associated, hand., transcription from bacterial-type RNA polymerase promoter, Nerve Cell, Long-Term Effects, 4931426K16Rik, ratio, forefoot of quadruped, screening, dTAF[[II]]230, wide/broad, DNA-dependent transcription, TAF200, Longterm Effect, Psychoses, Neurocognitive Disorder, terminal segment of free upper limb, Organic Mental Disorder, TAFII-250, TAF250/230, Spectrum Analysis, partial functionality, Spectroscopy, TAFII250, Mild Neurocognitive Disorder, AIDS, Dementia, Acquired Immune Deficiency Syndrome Virus, Nerve Cells, Lymphadenopathy-Associated, proportionality, Spectrometry, signs, rate, lacks function of type, CG17603, TAF[[II]], human, low functionality, organ system, Psychotic, wide, Taf250, SR3-5, hand region, bacterial transcription, Acquired Immunodeficiency Syndrome Virus, Cells, fore paw, forefoot, time, C6orf134, TAF230, Mental Disorder, Delirium, forelimb autopodium, Mild Neurocognitive Disorders, transcription, d230, human being, Peptidomics, Effects, Alpha-TAT1, Amnestic, Nerve, number, HTLV-III, impaired, Gene, dTAFII250, Spectrum Analyses, broad, protein-containing complex, EfW1, presence, body system, mec17, Human, period, polypeptide chain, dmTAF1, Taf230, CG18144, Mass, Gene Products, system, Human T-Cell Leukemia Virus Type III, Mass Spectroscopy, 0610011P08Rik, TAF250, proportion, Human T-Lymphotropic Virus Type III, Taf200, anatomical systems, dTAF[[II]]250, CLAMP, Viruses, Longterm, MEC17, cell, dysfunction, dHand, Immunodeficiency Viruses, PDZD1, Taf1p, causes, Clerambault Syndrome, Long-Term, Maintenances, Human T Cell Leukemia Virus Type III, man, Expressions, Dhand, dTAF250, hand-C, Disorder, Organic Brain Syndrome, causality, Lymphadenopathy-Associated Viruses, dHAND, Nonpsychotic, Neuron, HIV, Long-Term Effect, Expression, Human Immunodeficiency Viruses, TAF, Clamp, Mec17, 2.3.1.108, Lymphadenopathy-Associated Virus, Human Immunodeficiency Virus, cellular transcription, Traumatic Psychoses, Alpha-TAT, TAF[[II]]250, TAT, Human T Lymphotropic Virus Type III, findings, Neurocognitive Disorders, protein complex, Proteins, Organic Mental, l(3)84Ab, BG:DS00004.13, Cell, dTAF230, Traumatic, count in organism, Organic, native protein, natural protein, Immunodeficiency Virus, fore-paw, p230, Long Term Effects, Protein, TAF[[II]]250/230, TFIID, 2610110G12Rik, connected anatomical system, forefeet, Mass Spectrum Analyses, AIDS virus, Taf[[II]]250, Mass Spectrum, CAP70, TAF[[II]]230, AIDS Viruses, DmelCG18144, NHERF-3, DNA-templated, TAF[II]250, Understanding, LAV-HTLV-III, Longterm Effects, Hands, Protein Gene Products, Gene Proteins, having decreased function, Nbla00487, NHERF3, DmelCG17603, fore foot, Neurocognitive, quotient, forepaw, quantitative, Nonpsychotic Organic Brain Syndrome, Human T Cell Lymphotropic Virus Type III, presence or absence in organism, TAF1Organic Mental Disorders, Delirium, Mild Neurocognitive Disorders, Human T-Cell Lymphotropic Virus Type III, c6orf134, human being, Disorders, Effects, Alpha-TAT1, Nerve, Mild, Amnestic, AIDS Virus, HTLV-III, Human Immunodeficiency, mec17, Long Term, Human, Lymphadenopathy Associated Virus, period, Virus, cytopathology, Human T-Cell Leukemia Virus Type III, Effect, 0610011P08Rik, 3110080J08Rik, Human T-Lymphotropic Virus Type III, Viruses, Longterm, MEC17, Mild Neurocognitive, Immunodeficiency Viruses, Cognitive Disorders, Long-Term, Clerambault Syndrome, Human T Cell Leukemia Virus Type III, man, Acetyltransferase mec-17 homolog, Kandinsky Syndrome, Disorder, Pathologies, Organic Brain Syndrome, Lymphadenopathy-Associated Viruses, Nonpsychotic, Neuron, HIV, Long-Term Effect, 2610008K08Rik, Mental Disorders, associated, Human Immunodeficiency Viruses, Nerve Cell, Long-Term Effects, Mec17, 2.3.1.108, Lymphadenopathy-Associated Virus, Data Set., Human Immunodeficiency Virus, Alpha-TAT, Traumatic Psychoses, TAT, Human T Lymphotropic Virus Type III, Neurocognitive Disorders, Organic Mental, Longterm Effect, Psychoses, Neurocognitive Disorder, Organic Mental Disorder, Cell, Traumatic, Organic, Mild Neurocognitive Disorder, AIDS, Immunodeficiency Virus, Long Term Effects, 2610110G12Rik, AIDS virus, Dementia, Acquired Immune Deficiency Syndrome Virus, Nerve Cells, Lymphadenopathy-Associated, histopathology, AIDS Viruses, LAV-HTLV-III, human, Longterm Effects, Psychotic, Nbla00487, Neurocognitive, Acquired Immunodeficiency Syndrome Virus, Cells, biopsy, Nonpsychotic Organic Brain Syndrome, time, Human T Cell Lymphotropic Virus Type III, C6orf134, Mental Disorder254trueHIV-Tat treatment of human neurons in vitro over time: towards a model for the molecular pathology of HIV-associated neurocognitive disorders (~90% cell culture confluence dataset)We have used LC-MSMS discovery proteomics to identify the quantitative, cell-wide changes that occur when non-transformed, differentiated human neurons are treated with HIV-Tat over time. This may represent a novel cell culture model for initial HIV-associated neurocognitive disorder 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