Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_06.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_06.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_06.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_06.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_09.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_09.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_09.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_09.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_08.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_08.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_08.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_08.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_07.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g4_07.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g1_11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g2_07.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/ch_g3_07.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/Uniprot_HUMAN_042015.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007694/Hales-CTETMT-ProteomeDiscovererProcessedOutput.xlsbprimaryOK2000013050Eric DammerNot availableBrainBrain detergent insoluble fractions were generated as described previously from fresh frozen human lateral pre-frontal cortex [Bai B et al, PNAS, 2013; Hales CM et al, Mol Neurodegen, 2014]. All attempts were made to utilize the same region for each brain. In brief, approximately 200mg of frozen human frontal cortex was dounce homogenized (buffer: 50mM HEPES pH 7.0, 250mM sucrose, 1 mM EDTA, and 1x HALT (Pierce) protease inhibitor cocktail) followed by the addition of N-lauroylsarcosine (final 1% w/v) and NaCl (final 0.5 M). The sample was sonicated followed by ultracentrifugation at 180,000 x g. The pellet was solubilized in 8M urea buffer and protein concentrations were determined (BCA method, Pierce). Proteins were digested with trypsin and LC-MS/MS was performed as previously described except isobaric tandem mass tags were added to peptides to allow for multiplexing and quantification [Gokhale A et al, J. Neurosci, 2016]. Four batches of TMT 10-plex peptide mixtures, were prepated and mixed such that batch-confounding by disease status or CTE stage was avoided, and each peptide batch was offline fractionated via ERLIC solid phase for liquid chromatography. 11 ERLIC fractions for each batch were run on a Fusion Orbitrap (Thermo) mass spectrometer. At least one TMT-labeled channel in each batch was comprised of a multi-brain mixture representing all cases analyzed as a standard by which abundances across batches could be normalized.PrideChadwick M HalesOrbitrap Fusion ETDPARTIALDepartment of Neurology, Emory University, Atlanta, GA 3032229145658 Cherry JD, Zeineddin A, Dammer EB, Webster JA, Duong D, Seyfried NT, Levey AI, Alvarez VE, Huber BR, Stein TD, Kiernan PT, McKee AC, Lah JJ, Hales CM. Characterization of Detergent Insoluble Proteome in Chronic Traumatic Encephalopathy. J Neuropathol Exp Neurol. 2017 10.1093/jnen/nlx100Emory Universitycmhales@emory.eduMass SpectrometryShotgun proteomicsEncephalopathyDisease FreeAlzheimer's DiseaseProteostasisAdDetergent insoluble proteomeDementia pugilisticaHuman brain cteAlzheimer's diseasehttp://www.ebi.ac.uk/pride/archive/projects/PXD007694TMTiodoacetamide derivatized residuemonohydroxylated residuedeamidated residueAnalysis of MS/MS spectra for identification, and MS3 multinotch accumulated reporter ion intensities was performed as previously reported [Gokhale A et al, J. Neurosci, 2016], using Proteome Discover v2.1 (Thermo). Normalized log2 reporter ion intensity ratio versus the global multi-brain mixture was used to compare across cases and batches. Student’s t-test (two-tailed) was utilized to calculate p-values and significance for the differentially enriched or depleted proteins. Fold change comparison was calculated by subtracting the control log2 values from the CTE or AD log2 values.ProteomicsHomo Sapiens (human)edammer@emory.eduBiologicalUnited StatesQuantitative proteomics of postmortem human brain can identify dysfunctional proteins that contribute to neurodegenerative disorders like Alzheimer disease (AD) and frontotemporal dementia. Similar studies in chronic traumatic encephalopathy (CTE) are limited, therefore we hypothesized that proteomic sequencing of CTE frontal cortex brain homogenates from varying CTE pathologic stages may provide important new insights into this disorder. Quantitative proteomics of control, CTE and AD brains was performed to characterize differentially expressed proteins, and we identified over 4000 proteins in CTE brains, including significant enrichment of the microtubule associated protein tau. We also found enrichment and pathologic aggregation of RNA processing factors as seen previously in AD, supporting the previously recognized overlap between AD and CTE. In addition to these similarities, we identified CTE-specific enrichment of proteins which increase with increasing severity of CTE pathology. NADPH dehydrogenase quinone 1 (NQO1) was one of the proteins which showed significant enrichment in CTE and also correlated with increasing CTE stage. NQO1 demonstrated neuropathologic correlation with hyperphosphorylated tau in glial cells, mainly astrocytes. These results demonstrate that quantitative proteomic analysis of CTE postmortem human brain can identify disease relevant findings and novel cellular pathways involved in CTE pathogenesis.Characterization of Detergent Insoluble Proteome in Chronic Traumatic Encephalopathy.Cherry Jonathan D JD, Zeineddin Ahmad A, Dammer Eric B EB, Webster James A JA, Duong Duc D, Seyfried Nicholas T NT, Levey Allan I AI, Alvarez Victor E VE, Huber Bertrand R BR, Stein Thor D TD, Kiernan Patrick T PT, McKee Ann C AC, Lah James J JJ, Hales Chadwick M CMliquid chromatography tandem mass spectroscopy, CPD photolyase activity, PhrB photolyase activity, Ethylenediaminetetraacetic acid, nip, HEPES Monosodium Salt, Basodexan, protein, pigmented epithelium, Lobus Frontalis, (ethylenedinitrilo)tetraacetic acid, Bca, peptide, Polypeptides, solid, protein polypeptide chains, halite, peptido, ClvPrd, diseases, Detergents, B1, l(2)SH1330, Urea, 2, 3, AGM4, diseases and disorders, synganglion, NUP96, 2', outer pigmented layer of retina, Frontal, protein aggregate, epithelium, WMS, Edetic acid, insoluble, bca, HEPES Monosodium, 2-diyldinitrilo)tetraacetate, human disease, Frontal Cortex, LCMSMS, ion(4-), peptides, dermoid cyst with malignant transformation, N, E927b, Salt, pigmented retina, 35Bb, proteins, suprasegmental levels of nervous system, lobus frontalis, Frontal Cortices, DNA cyclobutane dipyrimidine photolyase activity, SUPPRESSOR OF AUXIN RESISTANCE 3, cloruro sodico, Brodmann Area 8, salt, pigment epithelium of retina, 1-Piperazineethanesulfonic acid, sample, pigmented retina epithelium, Homo sapiens disease, stage, Lyw-57, (ethane-1, GPHYSD2, acidum edeticum, SGS, LC-MS2, Brodmann's, PRE, ethylenediaminetetraacetate, rock salt, suprasegmental structures, lateral, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, detergent, LC-MS/MS, Liquid Chromatography, 2'', {[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID, pigmented retinal epithelium, Frontal Eye Fields, beta-D-fructofuranosyl, regio frontalis, ur, ACMICD, SLP65, carbamide, Ly-57, common salt, S-adenosyl-L-methionine:thiol S-methyltransferase activity, l(2)br3, l35Bb, batch, retinal pigment, frontal lobe cortex, Diseases, dipyrimidine photolyase (photosensitive), retinal pigment layer, region, SLP-65, frontal region, table sugar, positional polypeptide feature, BASH, 1-alpha-D-Glucopyranosyl-2-beta-D-fructofuranoside, LY57, retinal pigmented epithelium, Xylella fastidiosa str. Temecula1, Encephalon, alpha-D-Glucopyranoside, Brodmanns Area 8, frontal neocortex, MASS, N'-1, Supplementary, natrii chloridum, beta Trypsin, human, l(2)SH2 1330, disease, phr A photolyase activity, H2NC(O)NH2, Area 8, DNA-photoreactivating enzyme, Supplementary Eye Fields, liquid chromatography-tandem mass spectroscopy, BG:DS01219.1, Polypeptide, HEPES, TMT, 1728, cortex of frontal lobe, gray matter of frontal lobe, the brain, Lobe, other disease, Sucrose, human being, mol, region or site annotation, developmental stage, Pierce, FBN, beta-D-Fruf-(2<->1)-alpha-D-Glcp, Gene, Carbamide, ethylenediaminetetraacetic acid, photoreactivating enzyme activity, protein-containing complex, DmelCG4482, deoxyribodipyrimidine photolyase activity, EDTA tetraanion, stratum pigmentosum retinae, LC-MS-MS, Buffer, Karbamid, method, polypeptide chain, Ly57, ECTOL1, lobi frontales, Acide ethylenediaminetetracetique, method used in an experiment, Harnstoff, LC-MSMS, Brodmann's Area 8, Gene Products, disease or disorder, Sacharose, stratum pigmentosa retinae, Carmol, acide edetique, l(2)br23, positional, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, 2'''-(ethane-1, Saccharose, 2-Ethane diylbis-(N-(carboxymethyl)glycine), PRECOCIOUS, ethylenediamine tetraacetic acid tetraanion, Monosodium Salt, beta-Trypsin, frontal cortex, F23A5.3, chlorure de sodium, Frontal Eye Field, Eye Field, man, Xylella fastidiosa (strain Temecula1 / ATCC 700964), OCTD, non-neoplastic, edta, 2-diyldinitrilo)tetraacetic acid, EDTA, Kochsalz, site, disorder, peptidos, N-2-Hydroxyethylpiperazine-N'-2'-ethanesulfonic Acid, SUCROSE, 4-(2-hydroxyethyl)-, F23A5_3, Supplementary Eye Field, thiol methyltransferase activity, BLNK-S, CG4482, protein complex, Proteins, l(2)35Bb, disorders, medical condition, buffer, encephalon, Peptide, acido edetico, polypeptide, stratum pigmentosum (retina), MODIFIER OF SNC1, LC/MS/MS, Cortex, sacarosa, native protein, Natriumchlorid, natural protein, carbonyldiamide, Brodmann, sucrose, Protein, deoxyribonucleic photolyase activity, sequence, condition, table salt, MFS1, NaCl, grey matter of frontal lobe, WMS2, teratoma with malignant transformation, RPE, photolyase activity, uree, urea, H4edta, Tripcellim, N 2 Hydroxyethylpiperazine N' 2' ethanesulfonic Acid, CG15268, beta-D-fructofuranosyl alpha-D-glucopyranoside, retinal pigment epithelium, primary structure of sequence macromolecule, sample population, UREA, p. pigmentosa retinae, plan specification, Protein Gene Products, Mischung., br3, Gene Proteins, Trypure, Eye Fields, Cane sugar, SSKS, liquid chromatography tandem mass spectrometry, Peptid, deoxyribonucleate pyrimidine dimer lyase (photosensitive), NIP, edetic acid, Frontal Lobes, White sugar, ethylenediaminetetraacetate tetraanion, sodium chlorideinsoluble, Encephalopathies, Post Concussive, Traumatic Encephalopathy, Post Traumatic, Post-Concussive Encephalopathy, detergent, Post-Traumatic Encephalopathies, Post-Concussive Chronic Encephalopathy, total expressed protein, Post-Concussive Encephalopathies, Encephalopathy, Chronic Post-Concussive Encephalopathy., Post-Traumatic, Chronic Post Traumatic Encephalopathy, Chronic Post Concussive Encephalopathy, Chronic Post-Traumatic Encephalopathy, Chronic Post-Traumatic Encephalopathies, Post-Concussive Chronic Encephalopathies, Chronic Encephalopathy, Post Traumatic Encephalopathy, Post Concussive Encephalopathy, Detergents, Chronic Encephalopathies, Chronic, Chronic Post-Concussive Encephalopathies, Post-Traumatic Encephalopathy, Proteomes, Post-Concussivethe brain, proportion, Controlling, suprasegmental structures, ion, reference sample, determination, MS3, Ass-1, iones, Encephalon, Proteins, proportionality, total expressed protein, Gene, rate, tailed, proteins, Mischung, suprasegmental levels of nervous system, ions, encephalon, Protein Gene Products, ASS, Gene Proteins, caudal, Ionen, Ion, AA408052, chemical analysis, Protein, quotient, Gene Products, fold, synganglion, assay, Controlled., Proteomes, accumulated, ratioFamilial Pick's Disease, Disinhibition-Dementia-Parkinsonism-Amyotrophy, deceased, frontotemporal lobar Degeneration with Tau inclusions, frontotemporal dementia, naphthoquinone reductase activity, positive regulation by symbiont of host non-apoptotic programmed cell death, Wilhelmsen-Lynch Diseases, protein, Cardiac Death, neurotubule, Lobus Frontalis, dysfunctional, Disinhibition-Dementia-Parkinsonism-Amytrophy, protein polypeptide chains, Hereditary Dysphasic Disinhibition Dementia, diseases, Pick's Disease, DDPAC, Detergents, hemolysis by symbiont of host RBCs, symptoms, Complices, benzoquinone, 1, QR1, pathogenesis, Chronic Encephalopathies, 2, diseases and disorders, synganglion, cytopathology, Frontal, protein aggregate, dementia, with Parkinsonism, microtubulus, Frontotemporal Dementia with Parkinsonism, insoluble, NAD(P)H:quinone oxidoreductase activity, modification by symbiont of host biological process, human disease, Frontal Cortex, reference sample, stimulation by symbiont of host programmed cell death, Frontotemporal Lobe, NAD(P)H2 dehydrogenase (quinone), Wilhemsen-Lynch disease, Post-Concussive Chronic Encephalopathy, GRN-Related Frontotemporal, proteins, Familial, number of, suprasegmental levels of nervous system, lobus frontalis, Frontal Cortices, activation by organism of programmed cell death in other organism during symbiotic interaction, Chronic Post-Traumatic Encephalopathies, Brodmann Area 8, Ubiquitin-Positive Frontotemporal Dementia, Chronic Encephalopathy, Hereditary Dysphasic Disinhibition, NSC36324, Frontotemporal Lobe Dementias, Pathologies, PPP1R103, Homo sapiens disease, Wilhelmsen Lynch Disease, microtubuli, stage, p-benzoquinone reductase activity, cytolysis by organism of host cells, associated, AI413597, Brodmann's, screening, pallidopontonigral degeneration, quinone reductase activity, suprasegmental structures, Mtapt, Post Traumatic, detergent, Frontotemporal Lobe Dementias (FLDEM), extra or missing physical or functional parts, positive regulation by symbiont of host programmed cell death, Frontal Eye Fields, Frontotemporal Dementia with Parkinsonism-17, regio frontalis, Neuroglial Cell, results, partial functionality, Ubiquitin-Positive Frontotemporal, Semantic Dementias, Disinhibition-Dementia-Parkinsonism-Amytrophy Complex, enhancement of host programmed cell death, NAD(P)H(2) dehydrogenase (quinone) activity, Post Concussive Encephalopathy, frontal lobe cortex, Astrocyte, Ubiquitin-Positive Frontotemporal Dementias, AI551861, Diseases, modulation by symbiont of host system process, MSTD, overlap, Chronic Post-Concussive Encephalopathies, Diaphorase, Familial Pick's, Lobe Dementias, Wilhelmsen-Lynch disease, Microtubule, upregulation by symbiont of host programmed cell death., TPN Diaphorase, haemolysis in host, Dementia, GRN-Related Frontotemporal Dementia, NADPH, Frontotemporal Lobar Degeneration With Ubiquitin-Positive Inclusions, Disinhibition Dementia Parkinsonism Amyotrophy Complex, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complices, FTLD with TDP-43 Pathology, frontal region, histopathology, NMORI, dehydrogenase, Traumatic Encephalopathy, Encephalon, Brodmanns Area 8, perturbation by symbiont of host defense response, frontal neocortex, glial cell, signs, neuroglia, Dementias, Encephalopathy, Astroglial Cells, FTDP-17, lacks function of type, Semantic Dementia, Post-Traumatic, Supplementary, induction by organism of programmed cell death in other organism during symbiotic interaction, human, NAD(P)H:(quinone-acceptor)oxidoreductase activity, low functionality, Disinhibition Dementia Parkinsonism Amytrophy Complex, modification by symbiont of host morphology or physiology, Familial Pick's Diseases, NMOR1, NQO1, disease, regulation of cytolysis of host cells by symbiont, Area 8, menadione oxidoreductase activity, p-benzoquinone, induction by symbiont of host programmed cell death, Supplementary Eye Fields, hemolysis by symbiont of host red blood cells, diaphorase activity, activation by symbiont of host programmed cell death, MAPTL, frontotemporal lobe dementia (FLDEM), NAD(P)H: menadione oxidoreductase activity, reduced nicotinamide-adenine dinucleotide (phosphate) dehydrogenase activity, Lobe Dementia, FTLD-TDP, Post-Concussive, Proteomes, cortex of frontal lobe, viologen accepting pyridine nucleotide oxidoreductase activity, Glia, hyperphosphorylated, Frontotemporal Lobar Degeneration With Ubiquitin Positive Inclusions, gray matter of frontal lobe, Kolliker Reticulum, the brain, semantic variant PPA, Lobe, other disease, human being, Familial Picks Disease, Frontotemporal Lobe (FLDEM), Multiple System Tauopathy with Presenile Dementia, Encephalopathies, Dehydrogenase, regulation by symbiont of host system process, Post-Concussive Encephalopathy, developmental stage, modulation by organism of defense response of other organism involved in symbiotic interaction, number, impaired, Gene, Post-Concussive Encephalopathies, Astroglia Cells, 4-benzoquinone, protein-containing complex, with or without parkinsonism, presence, AW045860, HDDD2, HDDD1, Post-Concussive Chronic Encephalopathies, GRN-Related Frontotemporal Dementias, NAD(P)H-quinone dehydrogenase activity, flavoprotein NAD(P)H-quinone reductase activity, polypeptide chain, induction by organism of non-apoptotic programmed cell death in other organism during symbiotic interaction, Old Yellow, lobi frontales, Brodmann's Area 8, Gene Products, disease or disorder, Familial Pick Disease, FTD, Wilhelmsen-Lynch, Pick's Diseases, 5-cyclohexadiene-1, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complex, Ubiquitin Positive, Chronic Post-Concussive Encephalopathy, Astroglial Cell, induction of non-apoptotic programmed cell death by other organism, Disinhibition-Dementia-Parkinsonism-Amytrophy Complices, NADPH Diaphorase, reduced NAD(P)H dehydrogenase activity, dysfunction, Complex, frontal cortex, TPN, Frontal Eye Field, Eye Field, CK1epsilon, man, NADP, Chronic Post Traumatic Encephalopathy, Chronic Post Concussive Encephalopathy, FTLD-17 GRN, non-neoplastic, AW457082, Enzyme, AI426939, NADP Diaphorase, Glial Cells, FTD-PGRN, frontotemporal dementia with Parkinsonism, has or lacks parts of type, KC1epsilon, Naphthoquinone reductase activity, disorder, Chronic, Pallidopontonigral Degeneration, DT-diaphorase activity, NAD(P)H menadione reductase activity, Dia4, DIA4, Post-Traumatic Encephalopathy, Supplementary Eye Field, MTBT1, MTBT2, Controlled, Astroglia Cell, Disease, Controlling, phylloquinone reductase activity, Semantic, findings, disruption by symbiont of host cell, activation by organism of non-apoptotic programmed cell death in other organism, DHQU, Post Concussive, Tau, TAU, NADP Dehydrogenase, hemolysin activity, protein complex, quinone) activity, Proteins, disorders, Post-Traumatic Encephalopathies, multiple system tauopathy with presenile dementia, total expressed protein, PPND, medical condition, Frontotemporal Lobe Dementia (FLDEM), NADH-menadione reductase activity, vitamin K reductase activity, GRN-Related, encephalon, Cell, Wilhelmsen-Lynch Disease, disinhibition-dementia-Parkinsonism-amyotrophy Complex, mereological quality, menadione reductase activity, Frontotemporal Dementia, frontotemporal lobe dementia, count in organism, Ftdp17, Cortex, native protein, 4-dione, natural protein, tau, Frontotemporal Dementia with Parkinsonism 17, Astroglial, Brodmann, Astroglias, Protein, Frontotemporal Lobe Dementia, post-mortem, Old Yellow Enzyme, modulation by symbiont of host defense response, condition, FTD-GRN, vitamin-K reductase activity, DTD, induction by organism of programmed cell death in other organism involved in symbiotic interaction, mitigation by symbiont of host defense response, Ubiquitin-Positive, Death, frontotemporal, grey matter of frontal lobe, CKIe, Neuroglial Cells, frontotemporal lobar degeneration, GRN Related Frontotemporal Dementia, Neuroglia, enhancement of host programmed cell death by organism, Frontotemporal Dementias, semantic primary progressive aphasia, NSC-36324, Glial Cell, Frontotemporal, azoreductase activity, NAD(P)H dehydrogenase activity, Ftld with Tau inclusions, FTLD with TDP 43 Pathology, Chronic Post-Traumatic Encephalopathy, Protein Gene Products, NAD(P)H-quinone oxidoreductase activity, Gene Proteins, para-benzoquinone, having decreased function, Eye Fields, Pick Complex, Post Traumatic Encephalopathy, activation by organism of host programmed cell death, cardinality, NADPH:(acceptor) oxidoreductase, reduced nicotinamide adenine dinucleotide (phosphate, Astroglia, quantitative, biopsy, Frontal Lobes, presence or absence in organismFamilial Pick's Disease, Presenile Alzheimer Dementia, frontotemporal lobar Degeneration with Tau inclusions, Early Onset, Wilhelmsen-Lynch Diseases, Early Onset Alzheimer Disease, Lobus Frontalis, AD, protein polypeptide chains, DDPAC, symptoms, 1, Complices, benzoquinone, Chronic Encephalopathies, 2, Frontal, dementia, with Parkinsonism, NAD(P)H:quinone oxidoreductase activity, modification by symbiont of host biological process, stimulation by symbiont of host programmed cell death, early-onset, Frontotemporal Lobe, GRN-Related Frontotemporal, proteins, Familial, suprasegmental levels of nervous system, Focal Onset Alzheimer's Disease, lobus frontalis, Chronic Encephalopathy, AD1, Homo sapiens disease, Wilhelmsen Lynch Disease, stage, Brodmann's, screening, pallidopontonigral degeneration, quinone reductase activity, suprasegmental structures, Acute Confusional Senile Dementia, Post Traumatic, familial, Frontotemporal Lobe Dementias (FLDEM), Frontal Eye Fields, Neuroglial Cell, partial functionality, Alzheimer Type, Semantic Dementias, enhancement of host programmed cell death, NAD(P)H(2) dehydrogenase (quinone) activity, Post Concussive Encephalopathy, AI551861, Wilhelmsen-Lynch disease, TPN Diaphorase, haemolysis in host, Dementia, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complices, frontal region, NMORI, Alzheimer, perturbation by symbiont of host defense response, glial cell, signs, neuroglia, FTDP-17, Alzheimer-Type (ATD), Semantic Dementia, Supplementary, human, late-onset, Disinhibition Dementia Parkinsonism Amytrophy Complex, Familial Pick's Diseases, NMOR1, regulation of cytolysis of host cells by symbiont, Area 8, menadione oxidoreductase activity, p-benzoquinone, Senile Dementia, induction by symbiont of host programmed cell death, Supplementary Eye Fields, hemolysis by symbiont of host red blood cells, activation by symbiont of host programmed cell death, MAPTL, Alzheimer's Disease, Lobe Dementia, FTLD-TDP, Post-Concussive, cortex of frontal lobe, viologen accepting pyridine nucleotide oxidoreductase activity, Glia, hyperphosphorylated, Lobe, Familial Picks Disease, Alzheimers Diseases, Multiple System Tauopathy with Presenile Dementia, Encephalopathies, Peptidomics, Dehydrogenase, Sclerosis, regulation by symbiont of host system process, developmental stage, modulation by organism of defense response of other organism involved in symbiotic interaction, number, impaired, Astroglia Cells, Alzheimer disease 1, Focal Onset, protein-containing complex, HDDD2, HDDD1, Post-Concussive Chronic Encephalopathies, NAD(P)H-quinone dehydrogenase activity, flavoprotein NAD(P)H-quinone reductase activity, induction by organism of non-apoptotic programmed cell death in other organism during symbiotic interaction, Old Yellow, lobi frontales, Brodmann's Area 8, Gene Products, disease or disorder, FTD, Wilhelmsen-Lynch, Pick's Diseases, 5-cyclohexadiene-1, Disinhibition-Dementia-Parkinsonism-Amyotrophy Complex, NADPH Diaphorase, reduced NAD(P)H dehydrogenase activity, dysfunction, frontal cortex, TPN, Frontal Eye Field, man, NADP, Chronic Post Concussive Encephalopathy, FTLD-17 GRN, Presenile Dementia, Enzyme, NADP Diaphorase, Glial Cells, FTD-PGRN, KC1epsilon, Naphthoquinone reductase activity, Chronic, DT-diaphorase activity, NAD(P)H menadione reductase activity, Supplementary Eye Field, Astroglia Cell, Semantic, findings, disruption by symbiont of host cell, DHQU, Post Concussive, hemolysin activity, Proteins, disorders, vitamin K reductase activity, encephalon, Cell, Wilhelmsen-Lynch Disease, disinhibition-dementia-Parkinsonism-amyotrophy Complex, menadione reductase activity, Frontotemporal Dementia, frontotemporal lobe dementia, Cortex, native protein, 4-dione, Frontotemporal Dementia with Parkinsonism 17, proteomic analysis, condition, FTD-GRN, vitamin-K reductase activity, DTD, Ubiquitin-Positive, frontotemporal, Neuroglia, enhancement of host programmed cell death by organism, Frontotemporal Dementias, semantic primary progressive aphasia, Glial Cell, Frontotemporal, azoreductase activity, NAD(P)H dehydrogenase activity, Alzheimer Type Senile Dementia, Ftld with Tau inclusions, FTLD with TDP 43 Pathology, Alzheimer Sclerosis, NAD(P)H-quinone oxidoreductase activity, Gene Proteins, para-benzoquinone, having decreased function, Eye Fields, Pick Complex, activation by organism of host programmed cell death, Familial (FAD), reduced nicotinamide adenine dinucleotide (phosphate, protection against, Astroglia, quantitative, biopsy, Familial Alzheimer Diseases (FAD), Disinhibition-Dementia-Parkinsonism-Amyotrophy, frontotemporal dementia, naphthoquinone reductase activity, positive regulation by symbiont of host non-apoptotic programmed cell death, protein, neurotubule, dysfunctional, Primary Senile Degenerative, Disinhibition-Dementia-Parkinsonism-Amytrophy, Hereditary Dysphasic Disinhibition Dementia, diseases, Pick's Disease, hemolysis by symbiont of host RBCs, QR1, pathogenesis, diseases and disorders, synganglion, cytopathology, with cerebral amyloid angiopathy, protein aggregate, microtubulus, Frontotemporal Dementia with Parkinsonism, human disease, Frontal Cortex, Familial Alzheimer Disease (FAD), Alzheimer Diseases, reference sample, NAD(P)H2 dehydrogenase (quinone), Wilhemsen-Lynch disease, Post-Concussive Chronic Encephalopathy, Late Onset Alzheimer Disease, Frontal Cortices, activation by organism of programmed cell death in other organism during symbiotic interaction, Chronic Post-Traumatic Encephalopathies, Brodmann Area 8, Ubiquitin-Positive Frontotemporal Dementia, Hereditary Dysphasic Disinhibition, NSC36324, Frontotemporal Lobe Dementias, Alzheimer Type Dementia, early-onset familial form of Alzheimer disease, Pathologies, PPP1R103, microtubuli, p-benzoquinone reductase activity, cytolysis by organism of host cells, Presenile, associated, AI413597, Senile, Alzheimer disease, Mtapt, positive regulation by symbiont of host programmed cell death, Frontotemporal Dementia with Parkinsonism-17, regio frontalis, results, Ubiquitin-Positive Frontotemporal, Disinhibition-Dementia-Parkinsonism-Amytrophy Complex, frontal lobe cortex, Astrocyte, Ubiquitin-Positive Frontotemporal Dementias, Diseases, modulation by symbiont of host system process, MSTD, overlap, Chronic Post-Concussive Encephalopathies, Diaphorase, Familial Pick's, Lobe Dementias, Microtubule, upregulation by symbiont of host programmed cell death., GRN-Related Frontotemporal Dementia, NADPH, Frontotemporal Lobar Degeneration With Ubiquitin-Positive Inclusions, Disinhibition Dementia Parkinsonism Amyotrophy Complex, FTLD with TDP-43 Pathology, histopathology, dehydrogenase, Traumatic Encephalopathy, Encephalon, susceptibility to, Brodmanns Area 8, frontal neocortex, Dementias, Encephalopathy, Astroglial Cells, lacks function of type, Post-Traumatic, induction by organism of programmed cell death in other organism during symbiotic interaction, NAD(P)H:(quinone-acceptor)oxidoreductase activity, low functionality, modification by symbiont of host morphology or physiology, NQO1, disease, diaphorase activity, Acute Confusional, Alzheimer-Type Dementia (ATD), frontotemporal lobe dementia (FLDEM), NAD(P)H: menadione oxidoreductase activity, reduced nicotinamide-adenine dinucleotide (phosphate) dehydrogenase activity, Alzheimer Type Dementia (ATD), Frontotemporal Lobar Degeneration With Ubiquitin Positive Inclusions, gray matter of frontal lobe, Kolliker Reticulum, the brain, semantic variant PPA, other disease, human being, Frontotemporal Lobe (FLDEM), Post-Concussive Encephalopathy, Gene, Post-Concussive Encephalopathies, 4-benzoquinone, presence, with or without parkinsonism, AW045860, GRN-Related Frontotemporal Dementias, polypeptide chain, Familial Pick Disease, Ubiquitin Positive, Chronic Post-Concussive Encephalopathy, Astroglial Cell, induction of non-apoptotic programmed cell death by other organism, presenile and senile dementia, Disinhibition-Dementia-Parkinsonism-Amytrophy Complices, Complex, Eye Field, CK1epsilon, Chronic Post Traumatic Encephalopathy, non-neoplastic, AW457082, AI426939, Alzheimer Disease, frontotemporal dementia with Parkinsonism, disorder, Pallidopontonigral Degeneration, Dia4, DIA4, Post-Traumatic Encephalopathy, MTBT1, MTBT2, Controlled, Disease, Controlling, phylloquinone reductase activity, activation by organism of non-apoptotic programmed cell death in other organism, Tau, TAU, NADP Dehydrogenase, Alzheimer Syndrome, protein complex, quinone) activity, Post-Traumatic Encephalopathies, multiple system tauopathy with presenile dementia, PPND, medical condition, Frontotemporal Lobe Dementia (FLDEM), NADH-menadione reductase activity, GRN-Related, Primary Senile Degenerative Dementia, Alzheimer Dementias, count in organism, Ftdp17, natural protein, tau, Astroglial, Brodmann, Alzheimer's Diseases, Astroglias, Protein, Frontotemporal Lobe Dementia, Old Yellow Enzyme, modulation by symbiont of host defense response, induction by organism of programmed cell death in other organism involved in symbiotic interaction, mitigation by symbiont of host defense response, grey matter of frontal lobe, CKIe, Neuroglial Cells, frontotemporal lobar degeneration, GRN Related Frontotemporal Dementia, NSC-36324, Alzheimer Dementia, Chronic Post-Traumatic Encephalopathy, Protein Gene Products, Post Traumatic Encephalopathy, Late Onset, NADPH:(acceptor) oxidoreductase, Frontal Lobes, presence or absence in organisminsoluble, Encephalopathies, Post Concussive, Traumatic Encephalopathy, Post Traumatic, Post-Concussive Encephalopathy, detergent, Post-Traumatic Encephalopathies, Post-Concussive Chronic Encephalopathy, total expressed protein, Post-Concussive Encephalopathies, Encephalopathy, Chronic Post-Concussive Encephalopathy., Post-Traumatic, Chronic Post Traumatic Encephalopathy, Chronic Post Concussive Encephalopathy, Chronic Post-Traumatic Encephalopathy, Chronic Post-Traumatic Encephalopathies, Post-Concussive Chronic Encephalopathies, Chronic Encephalopathy, Post Traumatic Encephalopathy, Post Concussive Encephalopathy, Detergents, Chronic Encephalopathies, Chronic, Chronic Post-Concussive Encephalopathies, Post-Traumatic Encephalopathy, Proteomes, Post-Concussive1305trueCharacterization of detergent insoluble proteome in chronic traumatic encephalopathyProteomic sequencing of postmortem human brain can identify dysfunctional proteins that contribute to neurodegenerative disorders like Alzheimer’s disease and frontotemporal dementia. Similar studies in chronic traumatic encephalopathy are limited, but may provide important new insights into this disorder. Given our previous success with identifying pathology associated proteins, we performed proteomic sequencing of detergent insoluble brain homogenates from frontal cortex of deceased subjects with CTE covering a range of CTE pathologic stages. We compared the insoluble proteome of CTE brain to control and AD brains to identify differentially expressed proteins. We identified over 4000 proteins in CTE brains, including significant enrichment of the microtubule associated protein tau. We also found enrichment and pathologic aggregation of RNA processing factors as seen previously in AD, supporting the previously recognized overlap between AD and CTE. In addition to these similarities, we identified CTE-specific enrichment of a number of proteins which increase with increasing severity of CTE pathology. NADPH dehydrogenase quinone 1 (NQO1) was one of the proteins which showed significant enrichment in CTE and also correlated with increasing CTE stage. NQO1 demonstrated neuropathologic correlation with hyperphosphorylated tau in glial cells, mainly astrocytes. These results demonstrate that quantitative proteomic sequencing of CTE postmortem human brain can identify disease relevant findings and novel cellular pathways involved in CTE pathogenesis.2017-11-272017-09-08PXD007694280655164609918049851114542249215684354407128421832704236333635119834300663636275838569636312176666888811143015557114526314526236493461312695736664923916193641364510665688951136364762723661112329442229555832681304232312258636543460792519365910632143205971293865581912502321195774818661968215867225680761755437359253668408169180454278775120108922505836741479564100392369420494334459741420012935293NCBITaxon:776436715675936438677160540483131528269304562716391624292042779447623763696121810032962922113763948840479NCBITaxon:215713152731051067205968720165163684071736309698936104782148364320429294101061320491381693743277320464535111103217459216129288671288676410069456941016763410210761277351131658213584301417298174633675412873567568403013506218130194111929439367110411364459139498432025301270320221498751104759412047886134749515372110971652998279051355513980317585439801260866398081375755518320196447111415542193255521138343911931819813981810269701069955529NCBITaxon:47518502983354764146547798251021691572739823897648407413735186287054824153NCBITaxon:5055798446752510003643341475480210359741370811546654789835322159983810677103481985234878042253148841422286347946157187159919157295292921175271986014884063342495186912342352381523359858985988655974649987039202111072363527215987419082874341823502414511396929319025939071901746360123747463617226253600131972211915853105885969895110519899723798977200026341961026426672275885815689962873756269072442023717201999857072407476792745961533355431931167183355418400513686150596866628666134751536329NCBITaxon:17632862873488376705802718481865368595986075105135294296110098402321000734882412336363071047005187878945556449447NCBITaxon:13356265419591399878168119011478281055524NCBITaxon:96061358584690081979153519307285930085264114090051184995262254121337591390143811139442370416744312044143708351581990119519532590841370137023943222774862615835583715371633376057267998086371137121906503714759172073127262981274575727090309031372814243371421044792491108239412NCBITaxon:961514780269266100658137352750454135663816475203750644223669502129349790546322455525365199764990814389923745374726473237602647312887055955361416915252740327160293851149786479436375529382479437225117752991673871938441376593934327159100226376742344423454670012876895911984670414647929365280342803828035108034817939211262122950271131681911265269971028621076015973611431140981087135547743658142450722953379698204232124223321803369822347081645733185889716546759928777020382931412529550811481744888643745264999728303571830855071762997076789268370415156288765138856392456115208659555165518113549143213715974953502614321385878170768319496340161428005316461046603815184547448500974769747711805124017484185560155547920427420148871105325329726100594128909316435121160499001389438283542324233108293412205856041629913112273170187991542913126781818710493164072994673033373304852646383011306413992530642369923121162065261610337286129940207340115357652611114716133865431201725323712552329935316317NCBITaxon:11084993841063267872862321933486208621160791392227681196125522814953932132232334774121827532917573129948861814084341033519963139248811533811533933043937556992862633911209488624157183997586444237486435332444260665332413439097682127178637197985686399983998686553529148654104298635735825763288401986498238011191102868899958665220341632138119380625631886571303443112130886581144889198466645463NCBITaxon:11072NCBITaxon:11071142800628835374375116597145943116598854331116781077854314408043933434NCBITaxon:11082693977381382189931824431935128387145953116885311213278695berry8694100333586972443664701501114286921504751499105484204714725120847014649744497431170089306218644193061107632330805660396016217974825853946055396513212790568813377512981766017112147861050236694657349632051124501897441739412450128853183889198679488531911137910371647351660152943631631198573896939343237956026087121346334323223544314275241433611334565560291699636436802790105603140818838185733703401881274414437552162038713985584081724055986091838272759212744234081702759812744201274426235455382117627594328247480119915095648188229120455148167238473848190695148498101510693731263753405203860186497405224052141856451804NCBITaxon:8134780091522754847938703338493871917226175610946161435120325812055052352594473205493864651213880376686120678112202230743220118670511867057387334869121938791868023980071868012154514683514081861508471206777691838851608611388869152167786721279010087043512317196928123929486771332261262198131882682411229265716329576938124712461485451328388348392949113903631204162665890771514880036942694169436945187901015080817053942065421055687317447127015329242814111946691169601454586953201019812646905505371408157697392830312383911283128212802811010832825253531522811235315349825741948153623142410145481NCBInr 20130519294597064731292128857577398031309800891496978103690122020756601736548566156641036951187021187052261861299526945656217219565810102865606156596560644329428386567171360416023587001504802100291325291118700144771664180913252926997566556661348879379651431340223926633636336613833663367111764510378905923213143448709767468NCBITaxon:405597674651419842359093226411320498217870749821634725656905691569256934362872249821187205697111327949928993002014538661410NCBITaxon:49328715568910005898732135248304611308126373039114398872532897187268723872487298727135293324223110677410677330554831791988224454461041059098824974736776787524875217082485578428196194439105402104547815805968628606100264462NCBITaxon:5486815464143945033077348751526008759875632325933548130573710010772675672675664306154319474492394704721122984476119152361088767NCBITaxon:129788483139878286585974541293142898648012628348541898548756304113013259949529120491146247274601735272855525263852639119813330240976392465732252641109094674694163485131535231523845NCBITaxon:5659951736582200879325731399034651329196114644042479096109760616125732927294227294327294451338510516943146128017646571186961186941436183118698118691443906NCBITaxon:7771566338404330618519080256870325731025731156870861822305479186786618351350NCBITaxon:78039064515159903164515161040610943129257309193079109446091374944006198258741274707861923915NCBITaxon:71413095133434704483006419238900371590841281084069722823342810439201097797481140686251093928670620049291026339321034809109314140674109784257546013076376619108049267NCBITaxon:9874276891214822126719411462104905619922595767748371NCBITaxon:72271569743700312634065910901303276974114761309411470130813073832341147939811052685131413131311396553972101841455045399110098452627241530091076620411490130371344414398327470039884356239286683906144042119459999490336578618533658431275316273316277135280869135199488316275502779994871679718207954134966002713062744894831549675502780443239660025304292570212721349163349161135913581716741928434239549414717414718156269857301182590247149456092828513356269286757226404595374575740574113467611233523785481168511262161310915154293139613921390271489612881016010675744580240251229683352944139366581120866057625763443220667214082721200911408274282317729818431834432265755575757592810817066713640018399364442184922311210542111096976529818826331488373184319229438143124110497774438667825317513278042676085111022358801198732518519687916621411408224430491408223264561101411867631854312525985207677376777544580NCBITaxon:55710447226493298830NCBITaxon:1773101171346349231663367451011610688731328426265872542318829158195465488839194366123368144985505543826413103388451066541196327562532323168569134940988553264246210173660420845211856503157501851651571573545715751116234808637721631574962048867862962755231975825845853366851052311009110090100926465323406334062120079359610645391312575746924203069025937458046580422777919552491313662392410731300146625315684569374515479221198561198571462594NCBITaxon:592012676716248NCBITaxon:104385626994127915422231314781340388722921008810654976717589554930562791292491155748634291202531637616131477115200228569439717265072561177628766920262899315634301922344634338722276343642402062823667421386388420493211354724473144731169681112902551613983367225279310036NCBITaxon:11266NCBITaxon:439488186794414549413749901004163459106592106590NCBITaxon:662442711239014427010049801741028968833329549425282652158109434326521571279085256223727021654360507601435590763200359391305498008179536179535179534132234317953080221890364395019825289839642711305311925231177189587753460523NCBITaxon:289011218948932172697049803113180803014179813052114505338918789184NCBITaxon:9704749408049804057882259319140243427128373425613614179971408140629002813156737543313164163159100971462977467144675060139172614505111524962748749684605192602015800215381862562269275614231422275111927671450520101755275914282664179906914174609111797779408252824582762167539131463977852714273507145134073203696223283580712296213754513678303086783710945801580229535814621333524179819310946194202452787809036809933084581058111524260NCBITaxon:128056189618726351472640319520657467073853881354615821582390341390539034033815211809958335851149315242591130832263737NCBITaxon:127211488148558431043002451358472668342953195738261639945305861266835149814964521585545281132083751462089643795042089631379791387127283844006672032754532602644710880365867478434216599216597216595455032309821659621659116968331414532183217546271454361517203094980194547752555697815950943984525338551534398990371228577595156226231121088624455513108058884558707791817808431796758144571160488409859NCBITaxon:371626794015059076303555463263331199456545671025426420126420352486769674939254727933428146760945774449176048932191726525792501673428102395598632582788396591604903552527313366366666810242669102431024575741745268012675777641457966313670190832331156180895835510676657192763412359874445622066835070164002113615718006655648433767760207214413792997671400820140082111557110897244447899665349148624614749312659219292951946751299114127647363267656589363589366192011577271208370265554809664098614098609958676686337137221171101107687834974120280913485851348584502539249910861913249943311711395037117082711708281144522197822446147940210195375939723287581721874202566481219071NCBITaxon:57390507911654564526591774208321387NCBITaxon:615750521028307155864505912466635761311887631088878176179212190659407121906712111044975151950585066NCBITaxon:25068NCBITaxon:120651187036126344695060506150627249124643248784461229417101819430108673101851018457303963558633149246437151458430801321650855534825534812607101856871416333NCBITaxon:6191251643411218778127645159812847421635775534709514512257864250112607042607052607075730616584573677340541636774831672331505610071337056NCBITaxon:30555044571928751298722282912680631532734171929111167815022830NCBITaxon:208964283661742NCBITaxon:569382688306401794699138995915104352581515140129448242825844160419759272604232816772850120310267386492405360883858115589227054156224882936534036771861561088818817511935015075221193502167879108579172407132912122663060828805712561329013335503857917851310078752824NCBITaxon:610172433594633185862607991612432141262200298492670NCBITaxon:12637192812492125283038568385695911309632237561105025826662551519788360094338187146042235247214012575151111672085151546170445446415243235965489592022158010019995550884606157915781928650338921162089453593650917389440NCBITaxon:68544127541590109013410304158955210129661030612967142368715851584515849157592141038419286481928649369113388914792359046133296610914947616436902116022164133159936909108917688149229990894621812144640287889332952828294456861749342273218903821247190400727NCBITaxon:99139978282064111291671453720921535888167157546266940265619401372060328114319333291292479200129227257390872672710379085737292140532941281607839314262654898227377925390596207328827351431297936053307972115955640607241447335215094933493347936046747762903186256735670265669771933571580536076994688103761037741627638705200375842826831425685007123038364940813430749414422197857742171598921409239396211577811547569297932447047499061035919051581144527499073129877754699110358NCBITaxon:3729610360983341130798196432013367941336795783103401578658264536505911696547885469813431744586111952974553113131663430552132983603127612993850578505664261509901979401151345844577151346198351205623411676108077213487995696287782754466778721849620994887322184932097209620951441467761266447NCBITaxon:2697049509169369411031219588320509202906101580181306859458822499525297246741158933852894278942614774053560825673712334355141493174055345144514551471245466553341889251246791516413733450877111008323912903912952971962231274869317978911337310548763632712519850762554702225231187813865451655862238NCBITaxon:44664946493139458624212418730364926491141516784588108743952703195346232222690051671399669527106788954120030895442066563954530055862335518042643020030228481242642827283110299102981303873414452386262903195418329025862319557575584131016538235255772238235939946360106360104399483994786790054440423761082363557211622967718705473693128182451855794318682406672824932358255233271170621517271501261557228331314685349390NCBITaxon:101142845029780595231273541143733095988267427529595266437322430895938260943274959211222332951605201651087121697952528386972852904427421218707334747132342927816111921929760582181249885469584631220439733163910724373854588858725204936929554648038343691224326124193559552174939912816120425465519458228193329829725955711431792728435528571648120724139961660187625482964718761437868149049451472545725427254317610121480154107103187993618829455030341663360361659840505693528353528352527021529456698742972257247725859410976771679812310061911079450950412694322710279434472568291146136521871448542334937347373495270004466449917591891582580433302448046260499NCBITaxon:129391080612461962902224619732063752299169450906811879475723077412581175780329031114727279093110368399784459724028747512951391262469339952713252103430431962917264481381781893608108022815610054762557599553501112349192222197911119686619791210407281395108023010401459541619342110326441610511607111170227210411706343512744321085956210283128316683759729949514106588827114113161058413308794784475174293387709964326303396284179327793294172934106611457551392519232392320640131160620135666160621213324260533952851853847004787339591073996135674295105650024261911506218299633941548681346959753863121170519351626539002134188532216242515934433483348246882263863212196902501646860688512623477701666912151330030589910588450063434942160882357865222421574524672916132175126793406560464293311434280830018165236526212633761186175831678733450736544345076166690510894491089448NCBITaxon:2759653552071089444655066897447094287535NCBITaxon:5833300692906526659581211776266595311786654913497675192964417725784544417711581419NCBITaxon:12066381666117467329073651668446896573960169944446859600NCBITaxon:58111182263477154119165784584840215530756636164034624136810893112686341428099612NCBITaxon:5820NCBITaxon:582126531155319921150857975522836801986373791966272905896059606446709603227086266596496042929195705091210454330905042929829387834810612793654483851074454191701134899615266599369169275736407821963257075298351036171206189257771285023308352701427869991417158779627300251102856312673615286964696439644441768746128113450696381715229965696577575011238693517469662227909637875767842181921195623305952167426979613443463601345196789676673840967712495153304394032246200966996858355476789689342054557600968110428039320111678078330381719117630247593217181717475933NCBITaxon:102981257118387279694836470384766438467635688NCBITaxon:150270298207621158612184512107496866111495720928520131913840405295071263854614666383379NCBITaxon:10239173610629943630870547515851744575412NCBITaxon:1024528586NCBITaxon:1024317481565633788067067135622583313618550992452779658334240906112563049495NCBITaxon:63121770707010094086929257074876138176514894232903217602399352146752947462947474376031781446560285321773846451772177437272557272559153481121532369014726647091317047258520920269785962859631263871214697212035332648373153214693583706903074937608333483333570472851111149708333283331214687108018749200637373505104287828014851750NCBITaxon:1155213992751562259311726231656777351116032932853422NCBITaxon:5759345089998103517307796407148457425457428121536024271875058NCBITaxon:104075678024271535242915954126291589203985781355886755122965879309458041920312410515358153985801331678906902725602725612214272563354422841027256434166379017904NCBITaxon:5796356235552222104577575596995926367140370101364CP010395.1224218573232239230741964164027848964472020693771756614403666610400540296543NCBITaxon:1170922469764992297936941638284593556182284590143673340353143673452763922611117957527640985986661362652816230610442179622490541565050176946996NCBITaxon:13131712507953389427955795942197228722854219253066129457041767240418891220889320889520755966452315217531536879830200465672139269635889NCBITaxon:13156730195388399227166665553256657123051120755534131359022491179986669400666684215767114353531151042983154689111298593273075660469136165105612149161522178523897633612149159704126308213288818831313613NCBITaxon:1865381889081916073NCBITaxon:1865366873039376527306855743615295266891057675358231223NCBITaxon:13830595940458400358574291761608994291703002734054243232760568178606477816198215973997559980241994742757741559336237130368140734076667013388812041044111170711117082992040726563671396122114501344084408771117454797787454610740196794967957018640814082380394528135165966861235178616243231243230135992356127108978540964097978821035224327745157409097804092409344713529838615733178100816313625388654155401813416870436486979673239979371379548629916713033456433169173623125924851221282861109464722641988479451332103721284813173144077217913169315557781221119778219512012215225025518365231818600781131323848546456715916922591333499124961533454224327512047392432745365521340143649571657167849641673124966871601977162NCBITaxon:10357NCBITaxon:103581194881415784NCBITaxon:103591045856170721597652726341859347834113445826276261969471634955913645272621272623272624116071912389931214102132474403217142118213144032449032483431114778749093238481437113153681286640136841491859799491154373440302780832823525174299218951929524929492249241262673967315187661533489673012328013603190304143401191102249324934110205319795112111439190451866361713907710619813530085592921486917198252157728141444818759449593625NCBITaxon:11676224135163434291104549526798953627291456580000-0003-2947-7606