Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/proteinGroups.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/peptides.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/50mM-A-108-TG2575-18meses-06032017-5.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/50mM-A-108-TG2575-18meses-06032017-5.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/40mM-A-108-TG2575-18meses-06032017-4.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/100mM-A-108-TG2575-18meses-06032017-10.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/40mM-A-108-TG2575-18meses-06032017-4.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/30mM-A-108-TG2575-18meses-06032017-3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/20mM-A-108-TG2575-18meses-06032017-2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/10mM-A-108-TG2575-18meses-06032017-1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/60mM-A-108-TG2575-18meses-06032017-6.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/150mM-A-108-TG2575-18meses-06032017-11.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/100mM-A-108-TG2575-18meses-06032017-10.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/20mM-A-108-TG2575-18meses-06032017-2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/70mM-A-108-TG2575-18meses-06032017-7.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/10mM-A-108-TG2575-18meses-06032017-1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/90mM-A-108-TG2575-18meses-06032017-9.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/30mM-A-108-TG2575-18meses-06032017-3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/80mM-A-108-TG2575-18meses-06032017-8.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/300mM-A-108-TG2575-18meses-06032017-12.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/90mM-A-108-TG2575-18meses-06032017-9.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/70mM-A-108-TG2575-18meses-06032017-7.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/300mM-A-108-TG2575-18meses-06032017-12.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/80mM-A-108-TG2575-18meses-06032017-8.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/60mM-A-108-TG2575-18meses-06032017-6.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007795/150mM-A-108-TG2575-18meses-06032017-11.wiffprimaryOK200002810esantamma@navarra.esEnrique SantamarÃaMass SpectrometryShotgun proteomicsAlzheimer's DiseaseNot availableNeurodegenerationProteomicsOlfactory bulbAlzheimerhttp://www.ebi.ac.uk/pride/archive/projects/PXD007795BrainSample preparation for proteomic analysis – Murine OB specimens were homogenized in lysis buffer containing 7 M urea, 2 M thiourea, 50 mM DTT. The homogenates were spinned down at 100.000 x g for 1 h at 15ºC. Protein concentration was measured in the supernatants with the Bradford assay kit (Bio-rad). Protein Digestion and Peptide iTRAQ Labeling. A shotgun comparative proteomic analysis of OB proteomes using iTRAQ (isobaric Tags for Relative and Absolute Quantitation) was performed. Protein extracts were precipitated with Clean-up kit (Bio-Rad), and pellets dissolved in 7M urea, 2 M thiourea. Protein quantitation was performed with the Bradford assay kit (Bio-Rad). iTRAQ labeling of each sample was performed according to the manufacturer's protocol (Sciex). Briefly, equal amounts of OB proteins (50 μg) from each sample were reduced with 50 mM tris (2-carboxyethyl) phosphine (TCEP) at 60 °C for 1 h. Cysteine residues were alkylated with 200 mM methylmethanethiosulfonate (MMTS) at room temperature for 15 min. Protein enzymatic cleavage was carried out with trypsin (Promega; 1:20, w/w) at 37 °C for 16 h. Each tryptic digest was labelled according to the manufacturer's instructions with one isobaric amine-reactive tags as follows: Tag113, WT-1; Tag114, WT-2; Tag115, WT-3; Tag116, Tg2576-1; Tag117, Tg2576-2; Tag118, tg2576-3. After 2h incubation, the set of labelled samples were pooled and evaporated in a vacuum centrifuge. Liquid Chromatography tandem mass Spectrometry and data Analysis. To increase the proteome coverage, the peptide pool was submitted to cation exchange chromatography using spin Columns (Pierce). 12 fractions were collected (from 10mM to 150 mM KCl), evaporated under vacuum and reconstituted into10 μl of 2% acetonitrile, 0.1% formic acid, 98% MilliQ-H20 prior to mass spectrometric analysis. Peptide mixtures were separated by reverse phase chromatography using an Eksigent nanoLC ultra 2D pump fitted with a 75 μm ID column (Eksigent 0.075 x 250). Samples were first loaded for desalting and concentration into a 0.5 cm length 100 μm ID precolumn packed with the same chemistry as the separating column. Mobile phases were 100% water 0.1% formic acid (FA) (buffer A) and 100% Acetonitrile 0.1% FA (buffer B). Column gradient was developed in a 146 min two step gradient from 5% B to 25% B in 120 min and 25%B to 70% B in 15 min. Column was equilibrated in 95% B for 9 min and 5% B for 14 min. During all process, precolumn was in line with column and flow maintained all along the gradient at 300 nl/min. Eluting peptides from the column were analyzed using an Sciex 5600 Triple-TOF system. Information data acquisition was acquired upon a survey scan performed in a mass range from 350 m/z up to 1250 m/z in a scan time of 250 ms. Top 25 peaks were selected for fragmentation. Minimum accumulation time for MS/MS was set to 100 ms giving a total cycle time of 2.75 s. Product ions were scanned in a mass range from 100 m/z up to 1500 m/z and excluded for further fragmentation during 15 s.PrideiTRAQiTRAQ8plex-116 reporter+balance reagent acylated residuemonohydroxylated residuemethylthiolated residueData analysis. The raw MS/MS spectra search were processed using the freely available MaxQuant software (v.1.5.8.3) [20]. Initial maximum precursor and fragment mass deviations were set to 25 ppm and 0.5 Da, respectively. Variable modification (methionine oxidation and N-terminal acetylation) and fixed modification (MMTS) were set for the search and trypsin with a maximum of 1 missed cleavages was chosen for searching. The minimum peptide length was set to 7 amino acids and the false discovery rate (FDR) for PSM and protein identification was set to 1%. Matching against the protein sequence database was carried out after improving the precursor ion mass accuracy using the mass-dependent recalibration option of the software. The false-discovery rate was controlled at various levels by using a target-decoy search strategy, which integrates multiple peptide parameters such as length, charge, number of modifications and the identification score into a single quality that acts as the statistical evidence on the quality of each single peptide spectrum match. The frequently observed laboratory contaminants were removed and the protein identification was considered valid only when at least one unique or “razor” peptide was present. Following protein identification, the intensity for each identified protein was calculated using ion reporter intensities. The protein quantification was calculated using at least 2 razor + unique peptides, and calculation of statistical significance was carried out using two-way Student-t test (p < 0.05). A 1.3-fold change cutoff for all iTRAQ ratios (ratio ≤0.77 or ≥1.3) was selected to classify proteins as up- or down-regulated. Proteins with iTRAQ ratios below the low range (0.77) were considered to be underexpressed, whereas those above the high range (1.3) were considered to be overexpressed. The freely available software Perseus (version 1.5.6.0) [21] was used for statistical analysis and data visualizationProteomicsMultiomicsEnrique SantamariaTripleTOF 5600Proteomics Unit, Navarrabiomed-proteored (Spain)PARTIALMus Musculus (mouse)esantamma@navarra.es29077059 Palomino-Alonso M, Lachén-Montes M, González-Morales A, Ausín K, Pérez-Mediavilla A, Fernández-Irigoyen J, Santamaría E. Network-Driven Proteogenomics Unveils an Aging-Related Imbalance in the Olfactory IκBα-NFκB p65 Complex Functionality in Tg2576 Alzheimer's Disease Mouse Model. Int J Mol Sci. 2017 18(11) 10.3390/ijms18112260BiologicalBiomedicalProteomics unitSpainOlfaction is often deregulated in Alzheimer's disease (AD) patients, and is also impaired in transgenic Tg2576 AD mice, which overexpress the Swedish mutated form of human amyloid precursor protein (APP). However, little is known about the molecular mechanisms that accompany the neurodegeneration of olfactory structures in aged Tg2576 mice. For that, we have applied proteome- and transcriptome-wide approaches to probe molecular disturbances in the olfactory bulb (OB) dissected from aged Tg2576 mice (18 months of age) as compared to those of age matched wild-type (WT) littermates. Some over-represented biological functions were directly relevant to neuronal homeostasis and processes of learning, cognition, and behavior. In addition to the modulation of CAMP responsive element binding protein 1 (CREB1) and APP interactomes, an imbalance in the functionality of the IκBα-NFκB p65 complex was observed during the aging process in the OB of Tg2576 mice. At two months of age, the phosphorylated isoforms of olfactory IκBα and NFκB p65 were inversely regulated in transgenic mice. However, both phosphorylated proteins were increased at 6 months of age, while a specific drop in IκBα levels was detected in 18-month-old Tg2576 mice, suggesting a transient activation of NFκB in the OB of Tg2576 mice. Taken together, our data provide a metabolic map of olfactory alterations in aged Tg2576 mice, reflecting the progressive effect of APP overproduction and β-amyloid (Aβ) accumulation on the OB homeostasis in aged stages.Network-Driven Proteogenomics Unveils an Aging-Related Imbalance in the Olfactory IκBα-NFκB p65 Complex Functionality in Tg2576 Alzheimer's Disease Mouse Model.Palomino-Alonso Maialen M, Lachén-Montes Mercedes M, González-Morales Andrea A, Ausín Karina K, Pérez-Mediavilla Alberto A, Fernández-Irigoyen Joaquín J, Santamaría Enrique Esodium salt, projections, Kation, ammonium formate, 13C-labeled, MeCN, cadmium salt, chemical properties, muriate of potash, ion, fond, FEZL, rad, Basodexan, magnesium formate, CycEI, zinc salt, Long Term, thomson., l(2)k09905, 5730420M11Rik, Polypeptides, KL receptor activity, protein polypeptide chains, cobalt(II) formate dihydrate, CH3-C#N, GRP1, Grp1, SCO5, Urea, SCO1, Analysis, Gsfsow3, sylvite, WMS, ASD2, DmelCG8428, SET, Spin, Divorced, Analyses, weight-weight percentage, phosphatase 2A inhibitor I2PP2A, PTPSTEP, proteins, Divorces, W, AI047805, DmelCG4299, set, SPIN, decreased, column, scientific observation, BTKAP1, sample, Bs, autolysin activity, Half Cystine, GTFII-I, nickel salt, SGS, GPH, LC-MS2, 6030441I21Rik, Degradations, Klor-con, anatomical protrusion, chemical characterization, Zinc Cysteinate, cobalt (+2) salt, Longterm Effect, BAP135, Cyc E, not genetically inherited, ACMICD, PBT, sodium (4:1:1) salt, BG:DS07108.3, Dm1, magnesium salt, DmelCG42628, shelf, Raps, BAP-135, l(2)05206, Hydrogen Oxide, pbt, reticulate acropigmentation of Dohi, HLA-DR-associated protein II, DI-2, CG11628, I-2Dm, Protein Digestions, formate, dyschromatosis symmetrica hereditaria 1, Dmel_CG15720, pooled, cromium (+3), Striatum-enriched protein-tyrosine phosphatase, inhibitor of granzyme A-activated DNase, GtfII-I, study protocol, beta Trypsin, projection, ridge, w/w, I-2PP1, TAF-IBETA, l(2)W5, liquid chromatography-tandem mass spectroscopy, bacteriolytic toxin activity, ammonium (4:1) salt, TAF-Ibeta, Monopotassium chloride, methyl cyanide, lead (+2) salt, krk1, nickel formate dihydrate, GRP1/cytohesin 1, cycline, lead formate, Effects, lamellae, DmcyclinE, aluminum salt, Pierce, dyschromatosis symmetrica hereditaria, Carbamide, protein-containing complex, thomson, process of organ, body system, DmelCG5692, CG7826, mass-to-charge ratio, CG11633, cdi7, lamella, period, cyclinE, TOF, LC-MSMS, Gene Products, CG7835, Cdi7, CDI7, CG42273, system, Half-Cystine, Carmol, PH3, ZNF312, anatomical systems, pins, thallium (+1) salt, Longterm, L Cysteine, beta-Trypsin, rubidium salt, 2pp2a, Long-Term, methanoic acid, DmcycE, ions, study assay, PINS, CG10574, 2PP2A, dSET, dSet, peptidos, Chromatographies, laminae, chemical structure, chemical composition, WBSCR6, measuring, proto-oncogene c-Kit, mass percentage, CG5692, 1-(14)C-labeled, anatomical process, Proteins, Kationen, stepk, potassium formate, DSH, DSH1, copper (+2) salt, acetonitrile, buffer, ribosome-associated ubiquitin-dependent protein breakdown, Digestions, bnch, Kaliumchlorid, polypeptide, l(2)k02511, l(2)k02514, native protein, Temperatures, DmCycE, Proteolyses, carbonyldiamide, I-2PP2A, KIT ligand receptor activity, cupric formate, Dm I-2, chemical analysis, Long Term Effects, proteomic analysis, MFS1, aluminum formate, Pins, organ process, XKrk1, AU020952, lithium formate, holin, l(2)k02602, underdeveloped, Separations, hydrogen phosphorus, ethanenitrile, CD117, DmelCG11628, Longterm Effects, ME-IV, plan specification, Gene Proteins, processes, D-CycE, ZFP312, C-Kit, hydrogen phosphide, Ssm, xkl-1, CG4346, liquid chromatography tandem mass spectrometry, Data Analyses, liquid chromatography tandem mass spectroscopy, DIWS, IPP2A2, NCMe, determination, cation, zinc formate, KCl, lead salt, Xkl-1, protein, CG15720, lysis, peptide, Gsfsco1, ammonium tetraformate, ClvPrd, peptido, Ccne, Klotrix, Min, Williams-Beuren syndrome chromosomal region 6 protein, protein aggregate, Gsfsco5, present in fewer numbers in organism, Effect, DmelCG42273, SOW3, rsh, LCMSMS, peptides, TAF-I, TACHD, E927b, hypoplasia, min, mAPC, copper, SCAN, Protein Degradation, IGAAD, lithium salt, DmelCG10574, Diws1t, l(2)k08110, papilla, necrosis, Sl, GPHYSD2, ammonium (2:1) salt, Long-Term Effects, Dmel_CG4346, symmetric dyschromatosis of the extremities, phapii, [KCl], LC-MS/MS, lamina, Dmel_CG7826, StF-IT-1, flanges, Tr-kit, Th, br37, ur, any method, carbamide, Ionen, Digestion, 3.1.3.48, SRF-Phox1-interacting protein, kl1-A, bacteriocin activity, Kaon-Cl 10, KIT, nickel (+2) salt, Dmel_CG7835, Mnb, MNB, tyrosine-protein kinase Kit, l(2R)W5, ammonium salt, Step, ribosome-associated degradation, Xylella fastidiosa str. Temecula1, shelves, familial reticulate acropigmentation of Dohi, cobaltous formate, ribosome-associated ubiquitin-dependent protein degradation, L-Cysteine, kit, CG4299, MASS, chemical content, AW124434, REM3, organ system, H2NC(O)NH2, lysin activity, l35Dd, WBS, STEP, spine, copper salt, CG8428, Polypeptide, RAD1, i2pp2a, 1728, Proteomes, time, Cysteine Hydrochloride, Degradation, cesium salt, SCF receptor activity, rapsyn, FBN, Gene, cycE, TFII-I, l(2)br37, PHAPII, LC-MS-MS, CYCLE, Buffer, Bruton tyrosine kinase-associated protein 135, formic acid, protrusion, Karbamid, cytohesin/GRP1, method, potassium salt, scfr, polypeptide chain, reduced, template-activating factor I, subnumerary, ECTOL1, 14C-labeled, method used in an experiment, Harnstoff, IB291, tiny, AA673430, SCFR, Separated, Pro-Mega, Fdc, CYCE, DmelCG3938, iones, CyclE, ipp2a2, phosphane, l(2)SH2 0323, 3938, labeling, ridges, decreased number, calcium formate, Protein Degradations, Xylella fastidiosa (strain Temecula1 / ATCC 700964), OCTD, l(2)k05007, cations, dm-cycE, Neural-specific protein-tyrosine phosphatase, taf-ibeta, ribosome-associated ubiquitin-dependent protein catabolism, Cation, cromium (+3) salt, Long-Term Effect, BTK-associated protein 135, AA553322, FEZ, sodium formate, TCEP, small, DYRK1, data, CG42628, potassium chloride, nickel formate, 3H-labeled, protein complex, igaad, total expressed protein, strontium formate, acropigmentation of Dohi, Protein Digestion, strontium salt, Peptide, cationes, LC/MS/MS, natural protein, Protein, I2PP2A, Dyrk1, 25/11, Vacuums, CyeE, connected anatomical system, l(2)SH0323, ACETONITRILE, flange, cyanomethane, WMS2, CYH1, VSD1, Separation, c-KIT, uree, urea, CC1, l(2)35Dd, Tripcellim, Amine, sample population, chromic formate, UREA, Protein Gene Products, l(2)10403, Trypure, process, dSET/TAF-Ibeta, c-kit, 2610030F17Rik, Ion, Data, concentration, SSKS, calcium salt, processus, Peptid, RAD, Rad, assay, AA407739, CG3938Alzheimer Senile Dementia, Profiling, Protein kinase with no lysine 1, Presenile Alzheimer Dementia, Senile, Disease, Protein kinase lysine-deficient 1, ALZHEIMERS DIS, Researchs, Early Onset, Acute Confusional Senile Dementia, P65, 5430411C10Rik, GRASP65, mouse, Proteogenomic Profiling, KDP, Erythrocyte 65 kDa protein, Aging, Focal Onset, Alzheimer's disease, Alzheimer's Dementia, unspecified (disorder), Primary Senile Degenerative Dementia, [X]Dementia in Alzheimer's disease (disorder), Alzheimer Type, ALZHEIMER DIS, AD, 2.7.11.1, unspecified, Abnormal retropulsion test, HSAN2, hWNK1, PSK, [X]Dementia in Alzheimer's disease, Mus, Biological, p63, PPP1R167, p65, SYT, NOS, Proteogenomic Research, Imbalance, Balance impairment, Analysis, Kinase deficient protein, CG13176, Dementia, mice., Senescence, LATE ONSET ALZHEIMER DIS, Alzheimers, DAT - Dementia Alzheimer's type, Research, Dementia in Alzheimer's disease, DmelCG13176, Alzheimer, HSN2, Proteogenomic Analysis, PRKWNK1, Alzheimer's Disease Pathway, Dementias, Alzheimer Dementia, FOCAL ONSET ALZHEIMERS DIS, Biological Aging, NFKB3, Senile Dementia, Late Onset, Proteogenomic, AD - Alzheimer's disease, Alzheimer Disease, Alzheimer Type Dementia, Dementia in Alzheimer's disease (disorder), ALZHEIMER DIS EARLY ONSET, Mouse, SVP65, Alzheimer's Disease, Alzheimer's disease (disorder), house mouse, Presenile, Alzheimers Dementia, GOLPH5, Synaptotagmin I, SytI, Alzheimers disease, Dementia of the Alzheimer's typeSH2-Bb, Bru, IPP2A2, Raw, ion, Laboratory, Ass-1, Aminosaeure, Irip, Amino acid, protein, NAALAdase, 5730420M11Rik, peptide, Polypeptides, NAALADase I, L-Isomer Methionine, FOLH, protein polypeptide chains, Methionine, ClvPrd, peptido, AA408052, AA959943, fold, C530001K22Rik, Software Engineering, 3.4.17.21, Analysis, protein aggregate, Computer Program, WMS, Svc, SH2-B, Visualization, somitogenic mesoderm, SET, me75, F, amino acids, Data., peptides, reference sample, Analyses, Membrane glutamate carboxypeptidase, TAF-I, M, Open, phosphatase 2A inhibitor I2PP2A, Computer Programs and Programming, proteins, present in organism, NAALAD1, number of, D17Mit170, Folylpoly-gamma-glutamate carboxypeptidase, T1, Acts, DmelCG4299, ASS, set, IGAAD, DmelCG10574, ppm, GPHYSD2, Racemethionine, SGS, ratio, Acta-2, phapii, visualization, unsegmented mesenchyme, Aminokarbonsaeure, T5E21.12, acetylation, 2-Amino-4-(methylthio)butyric acid, StF-IT-1, Actsk-1, extra or missing physical or functional parts, Tl3, SH2-B PH domain-containing signaling mediator 1, Tl2, Source Softwares, alpha-amino carboxylic acids, Software Tools, Programs, ACMICD, Program, Computer Applications, Ionen, PSM, Psm, Computer Applications Software, Computer Applications Softwares, Methionin, methionine, laboratory, Softwares, Hmet, presumptive somite mesoderm, Prostate-specific membrane antigen, T5E21_12, parent ion, Amino Acid, Acid, Pteroylpoly-gamma-glutamate carboxypeptidase, Software Applications, HLA-DR-associated protein II, Amino acids, DI-2, Source Software, I-2Dm, FGCP, proportionality, rate, MASS, CG4299, inhibitor of granzyme A-activated DNase, beta Trypsin, I-2PP1, Pro-rich, data encoding as image, Applications, TAF-IBETA, precursor ion, PSMA, TAF-Ibeta, Acids, Polypeptide, i2pp2a, Liquimeth, Computer Software Applications, SH2 domain-containing protein 1B, 2-amino-4-(methylsulfanyl)butanoic acid, False, Aminocarbonsaeure, unsegmented paraxial mesoderm, number, L Isomer, FBN, Gene, Computer, precursor, alpha-amino acid, protein-containing complex, somitomeric mesoderm, GCPII, PHAPII, template-activating factor I, polypeptide chain, ECTOL1, Gene Products, L-Methionine, N-acetylated-alpha-linked acidic dipeptidase I, mKIAA1299, Del(8)44H, Low, Pedameth, Application, Open Source Softwares, SH2B, proportion, Software Application, iones, ipp2a2, beta-Trypsin, Open Source Software, Acetylations, 2pp2a, mGCP, 1.5.8.3, ions, CG10574, Computer Software Application, OCTD, 2PP2A, 2-amino-4-(methylthio)butanoic acid, Tools, 10^[-6], taf-ibeta, has or lacks parts of type, dSET, dSet, peptidos, metionina, statistical analysis, Controlled, Applications Software, Open Source, Controlling, Computer Software, cou, DL-Methionine, protein complex, Proteins, igaad, alpha-amino acids, L-Isomer, Peptide, Tool, GCP2, Glutamate carboxypeptidase II, mereological quality, polypeptide, Software Tool, Cell growth-inhibiting gene 27 protein, Lr, native protein, natural protein, I-2PP2A, Dm I-2, Protein, I2PP2A, sequence, MFS1, segmental plate, Sh2bpsm1, Software, WMS2, Met, Data Base, PH and SH2 domain-containing signaling mediator, Col4a-1, Engineering, Tripcellim, Amino, primary structure of sequence macromolecule, Folate hydrolase 1, Protein Gene Products, Computer Programs, Trypure, Gene Proteins, dSET/TAF-Ibeta, 2610030F17Rik, Applications Softwares, Ion, Data, SSKS, AI425885, cardinality, alpha-amino-gamma-methylmercaptobutyric acid, quotient, Bra, Peptid, SarDH, AA407739, Data AnalysesAmyloid intracellular domain 57, Forms, APP, Amyloid intracellular domain 59, ABETA, Bulbus Olfactorius, olfaction, Laboratory, Mus domesticus, A4, Gene Expression Profile, CTFgamma, CASP-14, protein, Profiles, Amyloid intracellular domain 50, House Mouse, Abpp, AAA, dysfunctional, Cvap, protein polypeptide chains, Personal, systemic amyloidosis, CG42318, AG, scent perception, diseases, Beta-amyloid protein 42, cerebral vascular amyloid peptide, Beta-amyloid protein 40, diseases and disorders, protein aggregate, amyloidogenic glycoprotein, AID(50), Main Olfactory Bulb, amyloidosis, human disease, Ag, Swiss Mice, proteins, Substance, Amyloid, olfactory lobe, Signatures, Alzheimer disease amyloid A4 protein homolog, N-APP, Gamma-secretase C-terminal fragment 50, Ongoing loss of nerve cells, Lateral Olfactory Tract, Gamma-secretase C-terminal fragment 57, Expression Signature, Gamma-secretase C-terminal fragment 59, AD1, Transcriptomes, Homo sapiens disease, TG, Abeta, Tracts, house mouse, APPI, C80, Neuro-degenerative disease, C83, close to, wide/broad, AICD-50, S-APP-alpha, Expression Profiles, mouse, CG17144, CR32097, Accessory Olfactory Bulbs, Gamma-CTF(57), partial functionality, Amyloid Fibrils, C99, Gene Expression, Expression Signatures, Mini-ICE, Diseases, AICD-57, Progressive neurodegenerative disorder, smell perception, Main Olfactory Bulbs, Expression Profile, AICD-59, Transcriptome Profiles, parent ion, Cerebral vascular amyloid peptide, Mus musculus, Bulbus, Amyloid Substance, amyloidoses, Caspase-14 subunit p10, mice, Swiss Mouse, Dignity, amyloid precursor protein, Caspase-14 subunit p19, alzheimer disease amyloid A4 protein homolog, olfactory lobe (Barr & Kiernan), sense of smell, Alzheimer disease amyloid protein, lacks function of type, Neurodegenerative disease, Protease nexin-II, MICE, Dmel_CG5620, human, low functionality, Lateral Olfactory, domesticus, disease, Lateral Olfactory Tracts, wide, DMDA, precursor ion, Patient, Olfactory Tracts, Mouse, Proteomes, amyloid-beta (A4) precursor protein, Elderly, 3.4.22.-, other disease, human being, Respect, amyloid-beta A4 protein, DmelCG42318, Transcriptome Profile, P3(40), impaired, amyloid beta A4 protein, Gene, mini-ICE, Amyloid Fibril, precursor, broad, protein-containing complex, betaApp, TYPE, AID(59), DAGA4, polypeptide chain, House, APP-C57, APP-C59, alzheimer disease amyloid protein, disease or disorder, protease nexin-II, Mus musculus domesticus, amyloid, Bulb, MAM, Mice, SCG3, PreA4, Swiss, Olfactory Bulbs, Profile, dysfunction, amyloidosis (disease), S-APP-beta, Dmel_CG17144, CG5620, Main Olfactory, Gamma-CTF(59), man, non-neoplastic, AID(57), Olfactory, Olfactory Glomerulus, Clients, Personal Respect, disorder, Olfactory Tract, Amyloidogenic glycoprotein, Transcriptome, protein complex, E030013M08Rik, disorders, total expressed protein, Accessory, medical condition, Client, Gamma-CTF(50), near to, APP-C99, native protein, natural protein, Mus, Adap, Protein, Gene Expression Signatures, condition, Bulbs, Gene Expression Signature, LGMD2C., Glomerulus, amyloid disease, Fibril, P3(42), PN-II, bulbus olfactorius (Morgagni), DMDA1, Olfactorius, House Mice, Soluble APP-beta, Accessory Olfactory Bulb, bulbus olfactorius, Laboratory Mice, C31, Tract, having decreased function, Fibrils, Lateral, Accessory Olfactory, Olfactory Bulb, Gene Expression Profiles, approaches, vicinity of, SCARMD2, Soluble APP-alpha, Beta-APP42, PN2, Beta-APP40, Signature, Laboratory Mouse, CVAP, Main, ABPPAmyloid intracellular domain 57, projections, Forms, Amyloid intracellular domain 59, Presenile Alzheimer Dementia, type 2, Bulbus Olfactorius, Protein kinase lysine-deficient 1, Laboratory, FALL39, Early Onset, GRASP65, A4, Profiles, Amyloid intracellular domain 50, House Mouse, Alzheimer's disease, AAA, Cvap, AD, protein polypeptide chains, unspecified, systemic amyloidosis, CG42318, AG, Abnormal retropulsion test, Biological, CAP-18, 2, AID(50), adenomas, Senescence, amyloidosis, L-methionine aminopeptidase activity, Ag, DmelCG13176, PRKWNK1, beta-amyloid, Nonmigrant, proteins, inhibition of homeostatic process, Transient, Signatures, Alzheimer disease amyloid A4 protein homolog, N-APP, Biological Aging, Gamma-secretase C-terminal fragment 50, Cognitions, Gamma-secretase C-terminal fragment 57, Expression Signature, Functions, Gamma-secretase C-terminal fragment 59, AD1, Tracts, Alzheimers disease, close to, anatomical protrusion, 3526402H21Rik, S-APP-alpha, Acute Confusional Senile Dementia, MYH-associated polyposis, CG17144, Alzheimer's Dementia, 5'-phosphate, Acceptance Processes, partial functionality, Alzheimer Type, HSAN2, Cyclic AMP, Expression Signatures, shelf, atomo, smell perception, atome, Expression Profile, Migrants and Transients, activation, Dementia, Cerebral vascular amyloid peptide, MUTYH-associated polyposis, MUTYH-related AFAP, RUTBC3, Amyloid Substance, amyloidoses, Caspase-14 subunit p10, Alzheimer, CHAMP, atoms, Swiss Mouse, pooled, Caspase-14 subunit p19, Neurodegenerative disease, Creb-1, projection, ridge, human, Lateral Olfactory, Lateral Olfactory Tracts, Senile Dementia, multiple colorectal, Migrants, MUTYH-related attenuated familial polyposis coli, Mouse, Alzheimer's Disease, adenosine 3', Elderly, 3.4.22.-, CRAMP, Protein kinase with no lysine 1, amyloid-beta A4 protein, DmelCG42318, lamellae, Processes, 5430411C10Rik, impaired, mini-ICE, Amyloid Fibril, precursor, Focal Onset, protein-containing complex, betaApp, process of organ, unspecified (disorder), AID(59), 5'-Cyclic AMP, ALZHEIMER DIS, lamella, AV083133, [X]Dementia in Alzheimer's disease, PPP1R167, Gene Products, Mus musculus domesticus, protease nexin-II, amyloid, Bulb, MAM, MAP, Phenomenography, PreA4, Olfactory Bulbs, Creb, Profile, ligand, familial adenomatous polyposis 2, dysfunction, CREB, S-APP-beta, beta amyloid, Dmel_CG17144, CG5620, Gamma-CTF(59), Worker, man, Cognitive Functions, AID(57), NFKB3, anatomical unit, transient structure, body organ, negative regulation of homeostatic process, SVP65, laminae, atom, Drl, Amyloidogenic glycoprotein, 2310001E10Rik, anatomical process, Proteins, Mdu, β-amyloid, near to, native protein, 5'-cyclic phosphate, mda, MUTYH-related attenuated FAP, Gene Expression Signatures, Balance impairment, Glomerulus, regulation of homeostatic process, amyloid disease, ami, organ process, Fibril, bulbus olfactorius (Morgagni), DMDA1, Olfactorius, increased number, med, Function, House Mice, C31, Tract, processes, Gene Proteins, having decreased function, Fibrils, Accessory Olfactory, Gene Expression Profiles, Nomads, Dementia in Alzheimer's disease (disorder), vicinity of, ADENOSINE-3', MAP syndrome, Beta-APP42, PN2, Beta-APP40, Main, ABPP, APP, ABETA, Cognitive Function, ALZHEIMERS DIS, olfaction, methionine aminopeptidase activity, 5'-cyclic monophosphate, Mus domesticus, Gene Expression Profile, CTFgamma, CASP-14, KDP, protein, Erythrocyte 65 kDa protein, Aging, Abpp, element, CG11121, dysfunctional, old, scent perception, Beta-amyloid protein 42, cerebral vascular amyloid peptide, Beta-amyloid protein 40, protein aggregate, amyloidogenic glycoprotein, C13orf8, peptidase M activity, Kinase deficient protein, Main Olfactory Bulb, increased, rabGAPLP, LATE ONSET ALZHEIMER DIS, Migrant Worker, DAT - Dementia Alzheimer's type, cAMP, ZNF828, 3', RabGAP-5, Swiss Mice, behavioral response to stimulus, Substance, Amyloid, olfactory lobe, SO, Ongoing loss of nerve cells, Lateral Olfactory Tract, AD - Alzheimer's disease, RUSC3, papilla, Alzheimer Type Dementia, Transcriptomes, Squatters, homeostasis, TG, Abeta, ALZHEIMER DIS EARLY ONSET, APPI, Presenile, C80, Neuro-degenerative disease, So, C83, single-organism behavior, Senile, wide/broad, AICD-50, Process, Expression Profiles, mouse, lamina, autosomal recessive familial adenomatous polyposis, flanges, Maps, CR32097, CAP18, Accessory Olfactory Bulbs, Gamma-CTF(57), CAMP, Amyloid Fibrils, C99, Gene Expression, Acceptance Process, positive regulation of homeostatic process, PSK, Migrant, Mini-ICE, SYT, AICD-57, Progressive neurodegenerative disorder, NOS, Workers, Main Olfactory Bulbs, AICD-59, autosomal recessive, Transcriptome Profiles, parent ion, Mus musculus, Bulbus, Alzheimers, Element, mice, shelves, Dementias, amyloid precursor protein, alzheimer disease amyloid A4 protein homolog, olfactory lobe (Barr & Kiernan), sense of smell, Alzheimer disease amyloid protein, lacks function of type, MICE, Protease nexin-II, Dmel_CG5620, low functionality, domesticus, RABGAP5, wide, DMDA, precursor ion, Patient, spine, Olfactory Tracts, Adenosine 3', Proteomes, amyloid-beta (A4) precursor protein, Synaptotagmin I, accessory, Dementia of the Alzheimer's type, human being, Transcriptome Profile, P3(40), P65, amyloid beta A4 protein, Gene, broad, supernumerary, TYPE, Migrant Workers, protrusion, DAGA4, hWNK1, polypeptide chain, House, Cyclic adenylic acid, DmelCG11121, APP-C57, p63, Elderly., APP-C59, p65, alzheimer disease amyloid protein, Imbalance, Mice, SCG3, familial adenomatous polyposis, somda, Swiss, Transients, amyloidosis (disease), Nonmigrants, Main Olfactory, ridges, FOCAL ONSET ALZHEIMERS DIS, autosomal recessive multiple colorectal adenomas, Squatter, Olfactory, Olfactory Glomerulus, Alzheimer Disease, Clients, Alzheimer's disease (disorder), Olfactory Tract, elements, Behaviors, Alzheimers Dementia, behaviour, SytI, Autoregulation, Alzheimer Senile Dementia, MUTYH-related attenuated familial adenomatous polyposis, Disease, data, Transcriptome, MUTYH-Associated Polyposis, protein complex, E030013M08Rik, total expressed protein, Accessory, Client, Primary Senile Degenerative Dementia, LGMD2C, cognitive function, [X]Dementia in Alzheimer's disease (disorder), Gamma-CTF(50), APP-C99, 2.7.11.1, natural protein, Mus, Adap, Protein, Nomad, Bulbs, atomus, Gene Expression Signature, flange, CG13176, Acceptance, P3(42), PN-II, LL37, FALL-39, Dementia in Alzheimer's disease, HSN2, Alzheimer's Disease Pathway, 5'-(hydrogen phosphate), Soluble APP-beta, Accessory Olfactory Bulb, MYH-Associated Polyposis, Cognitive, Alzheimer Dementia, bulbus olfactorius, 5'-CYCLIC-MONOPHOSPHATE, Laboratory Mice, Protein Gene Products, process, present in greater numbers in organism, Lateral, activation of homeostatic process, behavioural response to stimulus, Late Onset, Olfactory Bulb, approaches, SCARMD2, Soluble APP-alpha, processus, Signature, responsive, Laboratory Mouse, GOLPH5, CVAP, FAP2, colorectal adenomatous polyposis3.4.22.-, Mus musculus, Caspase-14 subunit p10, Peptidomics, Laboratory, Swiss, mice, Mus domesticus, Swiss Mouse, mouse, mini-ICE, House Mice, Swiss Mice, Caspase-14 subunit p19, CASP-14, MICE, House Mouse, Laboratory Mice, domesticus, House, Mus, Mini-ICE, Mus musculus domesticus, Mouse, Mice, Laboratory Mouse, Mus musculus., Elderly281trueolfactory proteomics in aged Tg2576 miceOlfaction is often deregulated in Alzheimer´s disease (AD) patients, being also impaired in transgenic Tg2576 AD mouse model, which overexpress the Swedish mutated form of human amyloid precursor protein (APP). However, little is known about the molecular mechanisms that accompany the neurodegeneration of olfactory structures in Tg2576 mice. For that, we have applied proteome- and transcriptome-wide approaches to probe molecular disturbances in the olfactory bulb (OB) dissected from aged Tg2576 mice (18 months of age) respect to age matched wild-type (WT) littermates2017-11-022017-09-20PXD0077952806551646099180498511145422492156843544071284218327042363336351198343006636362758385696363121766668888111430155571145263145262364934613126957366649239161936411066568895113636476272366111232944222955583232312258636543460792519365910632143205971293865581912502321195774818661968215867225680761755437359253668408169180454278775120108922505836741479564100392369420494334459741420012935293NCBITaxon:776436715675936438677160540483131528269304562716391624292042779447623763696121810032962922113763948840479NCBITaxon:2157131527310510672059687201651636840717363096989361047821483643204292941010613204913816937432773204645351111032174592161292886712886764100694569410167634102107612773511316582135843014172981746336754128735675684030135062181301941119294393671104113644591394984320253012703202214987511047594120478861347495153721109716529982790513980317585439801260866398081375755518320196447111415542193255521138343911931819813981810269701069955529NCBITaxon:47518502983354764146547798251021691572739823897648407413735186287054824153NCBITaxon:50557984467525100036433414754803597413708115466547898353221599838106771034819852348780422531488414222863479461571871599191572952929211752719860148840633424951869123423515233598589859886559746499870392021110723635272159874190828743418235024145113969293190259390719017463601237474636172262536001319722119158531058859698951105198997237989772000263419610264266722758858156899628737562690724420237172019998570724074767927459615333554319313355418400513686150596866628666134751536329NCBITaxon:17632862873488376705802718481865368595986075105135294296110098402321000734882412336363071047005187878945556449447NCBITaxon:13356265419591399878168119011478281055524NCBITaxon:96061358584690081979153519307285930085264114090051184995262254121337591390143811139442370416744312044143708351581990119519532590841370137023943222774862615835583715371633376057267998086371137121906503714759172073127262981274575727090309031372814243371421044792491108239412NCBITaxon:96151478026926610065813735275045413566381647520375064422366950212934979054632245552536519976499081438992374537472647323760264731288705595536252740327160293851149786479436375529382479437225117752991673871938441376593934327159100226376742344423454670012876895911984670414647929365280342803828035108034817939211262122950271131681911265269971028621076015973611431140981087135547743658142450722953379698204232124223321803369822347081645733165467599287770203829314125295508114817448886437452649997283035718308550717629970767892683704151562887651388563924561152086595551655181135491432137159749535026143213858781707683194963401614280053164610466038151845474485009747697477118051240174841855601555479204274201488711053253297261005941289093164359900138943828354232423310829341220585604162991311227317018799154291312678181871049316407299467303337330485264638301130641399253064236121162065261610337286129940207340115357652611114716133865431201725323712552329935316317NCBITaxon:110849938410632678728623219334862086211607913922276811961255228149539321322323347741218275329175731299488618140843410335199631392488115338115339330439375569928626339112094886241571839975864442374864353324442606653324134390976821271786371979856863999839986865535291486541042986357358257632884019864982380102868899958665220341632138119380625631886571303443112130886581144889198466645463NCBITaxon:11072NCBITaxon:11071142800628835374375116597145943116598854331116781077854314408043933434NCBITaxon:11082693977382189931824431935128387145953116885311213278695berry86941003335869724436647015011142869215047514991054842047147251208470146497444974311700893062186441930611076323308056603960162179748258539460553965132127905688133775129817660171121478610502366946532051124501897441739412450128853183889198679488531911137910371647351660152943631631198573896939343237956026087121346334323223544314275241433611334565560291699636436802790105603140818838185733703401881274414437552162038713985584081724055986091838272759212744234081702759812744201274426235455382117627594328247480119915095648188229120455148167238473848190695148498101510693731263753405203860186497405224052141856451804NCBITaxon:8134780091522754847938703338499172261756109461614351203258120550523525944732054938646512138803766861206781122022307418670511867057387334869121938791868023980071868012154514683514081861508471206777691838851608611388869152167786721279010087043512317196928123929486771332261262198131882682411229265716329576938124712461485451328388348392949113903631204162665890771514880036942694169436945187901015080817053942065421055687317447127015329242814111946691169601454586953201019812646905505371408157697392830312383911283128212802811010832825253531522811235315349825741948153623142410145481NCBInr 20130519294597064731292128857577398031309800891496978103690122020756601736548566156641036951187021187052261861299526945656217219565810102865606156596560644329428386567171360416023587001504802100291325291118700144771664180913252926997566556661348879379651431340223926633636336613833663367111764510378905923213143448709767468NCBITaxon:405597674652359093226411320498217870749821634725656905691569256934362872249821187205697111327949928993002014538661410NCBITaxon:49328715568910005898732135248304611308126373039114398872532897187268723872487298727135293324223110677410677330554831791988224454461041059098824974736776787524875217082485578428196194439105402104547815805966100264462NCBITaxon:5486815464143945033077348751526008759875632325933548130573710010772675672675664306154319474492394704721122984476119152361088767NCBITaxon:1297884831398782865859745412931428986480126283485418985487563041259949529120491146247274601735272855525263852639119813330240976392465732252641109094674694163485131535231523845NCBITaxon:5659951736582200879325731399034651329196114644042479096109760616125732927294227294327294451338510516943146128017646571186961186941436183118698118691443906NCBITaxon:7771566338404330618519080256870325731025731156870861822305479186786618351350NCBITaxon:78039064515159903164515161040610943129257309193079109446091374944006198258741274707861923915NCBITaxon:7141309513343470448300641923890037159084128108406972282334281043920109779748114068628670620049291026339321034809109314140674109784257546013076376619108049267NCBITaxon:9874276891214822126719411462104905619922595767748371NCBITaxon:7227156974370031263406591090130327697411476130941147013081307383234114793981105268513141313131139655397210184145504539911009845262724153009107662041149013037134441439832747003988435623928668390614404211945999949033657861853365843127531627331627713528086913519948831627550277999487167971820795413496600271306274489483154967550278044323966002530429257021272134916334916113591358171674192843423954941471741471815626985730118259024714945609282851335626928675722640459537457574057411346761123352378548116851126216131091515429313961392139027148961288101601067574458024025122968335294413936658112086605762576344322066721408272120091140827428231772981843183443226575557575759281081706674442184922311210542111096976529818826331488373184319229438143124110497774438667825317513278042676085111022358801198732518519687916621411408224430491408223264561101411867631854312525985207677376777544580NCBITaxon:5571044722649329NCBITaxon:17731011713463492316633674510116106887313284262658725423188291581954654888391943661233681449855055438264131033884510665411963275625323231685691349409885532642462101736604208452118565031575018516515715735457157511162348086377216315749620488678629627552319758258458533668510523110091100901009264653234063340621200793596106453913125757469242030690259374580422777919552491313662392410731300146625315684569374515479221198561198571462594NCBITaxon:592012676716248NCBITaxon:104385626994127915422231314781340388722921008810654976717589554930562791292491155748634291202531637616131477115200228569439717265072561177628766920262899315634301922344634338722276343642402062823667421386388420493211354724473144731169681112902551613983367225279310036NCBITaxon:11266NCBITaxon:439488186794414549413749901004163459106592106590NCBITaxon:6624427112390144270100498017410289688333295494252826521581094343265215712790852562237270216543605076014355907632003593913054980081795361795351795341322343179530802218903643950198252898396427113053119252311771895877534605231218948932172697049803113180803014179813052114505338918789184NCBITaxon:9704749408049804057882259319140243427128373425613614179971408140629002813156737543313164163159100971462977467144675060139172614505111524962748749684605192602015800215381862562269275614231422275111927671450520101755275914282664179906914174609111797779408252824582762167539131463977852714273507145134073203696223283580712296213754513678303086783710945801580229535814621333524179819310946194202452787809036809933084581058111524260NCBITaxon:128056189618726351472640319520657467073853881354615821582390341390539034033815211809958335851149315242591130832263737NCBITaxon:12721148814855843104300245132668342953195738261639945305861266835149814964521585545281132083751462089643795042089631379791387127283844006672032754532602644710880365867478434216599216597216595455032309821659621659116968331414532183217546271454361517203094980194547752555697815950943984525338551534398990371228577595156226231121088624455513108058884558707791817808431796758144571160488409859NCBITaxon:371626794015059076303555463263331199456545671025426420126420352486769674939254727933428146760945774449176048932191726525792501673428102395598632582788396591604903552527313366366666810242669102431024575741745268012675777641457966313670190832331156180895835510676657192763412359874445622066835070164002113615718006655648433767760207214413792997671400820140082111557110897244447899665349148624614749319292951946751299114127647363267656589363589366192011577271208370265554809664098614098609958676686337137221171101107687834974120280913485851348584502539249910861913249943311711395037117082711708281144522197822446147940210195375939723287581721874202566481219071NCBITaxon:57390507911654564526591774208321387NCBITaxon:615750521028307155864505912466635761311887631088878176179212190659407121906712111044975151950585066NCBITaxon:25068NCBITaxon:120651187036126344695060506150627249124643248784461229417101819430108673101851018457303963558633149246437151458430801321650855534825534812607101856871416333NCBITaxon:619111218778127645159812847421635775534709514512257864250112607042607052607075730616584573677340541636774831672331505610071337056NCBITaxon:30555044571928751298722282912680631532734171929111167815022830NCBITaxon:208964283661742NCBITaxon:56938268830640179469913899591510435258151514012944824282584416041975927260423281677285012031026738649240536088385811558922705415622488293653403677186156817511935015075221193502167879108579172407132912122663060828805712561329013335503857917851310078752824NCBITaxon:610172433594633185862607991612432141262200298492670NCBITaxon:12637192812492125283038568385695911309632237561105025826662551519788360094146042235247214012575151111672085151546170445446415243235965489592022158010019995550884606157915781928650338921162089453593650917389440NCBITaxon:68544127541590109013410304158955210129661030612967142368715851584515849157592141038419286481928649369113388914792359046133296610914947616436902116022164133159936909108917688149229990894621812144640287889332952828294456861749342273218903821247190400727NCBITaxon:991399782820641112916714537209215358157546266940265619401372060328114319333291292479200129227257390872672710379085737292140532941281607839314262654898227377925390596207328827351431297936053307972115955640607241447335215094933493347936046747762903186256735670265669771933571580536076994688103761037741627638705200375842826831425685007123038364940813430749414422197857742171598921409239396211577811547569297932447047499061035919051581144527499073129877754699110358NCBITaxon:3729610360983341130798196432013367941336795783103401578658264536505911696547885469813431744586111952974553113131663430552132983603127612993850578505664261509901979401151345844577151346198351205623411676108077213487995696287782754466778721849620994887322184932097209620951441467761266447NCBITaxon:2697049509169369411031219588320509202906101580181306859458822499525297246741158933852894278942614774053560825673712334355141493174055345144514551471245466553341889251246791516413733450877111008323912903912952971962231274869317978911337310548763632712519850762554702225231187813865451655862238NCBITaxon:446649464931394586242124187303649114151678458810874395270319534623222269005167139966952710678895412003089544206656395453005586233551804264302003022848124264282728311029910298130387341445238626290319541832902586231955757558413101653823525577223823593994636010636010439948399478679005444042376108236355721162296771870547369312818245185579431868240667282493235825523327117062151261557228331314685349390NCBITaxon:101142845029780595231273541143733095988267427529595266437322430895938260943274959211222332951605201651087121697952528386972852904427421218707334747132342927816111921929760582181249885469584631220439733163910724373854588858725204936929554648038343691224326124193559552174939912816120425465519458228193329829725955711431792728435528571648120724139961660187625482964718761437868149049451472545725427254317610121480154107103187993618829455030341663360361659840505693528353528352527021529456698742972257247725859410976771679812310061911079450950412694322710279434472568291146136521871448542334937347373495270004466449917591891582580433302448046260499NCBITaxon:129391080612461962902224619732063752299169450906811879475723077412581175780329031114727279093110368399784459724028747512951391262469339952713252103430431962917264481381781893608108022815610054762557599553501112349192222197911119686619791210407281395108023010401459541619342110326441610511607111170227210411706343512744321085956210283128316683759729949514106588827114113161058413308794784475174293387709964326303396284179327793294172934106611457551392519232392320640131160620135666160621213324260533952851853478733959135674295105650024261911506218299633941548681346959753863121170519351626539002134188532216242515934433483348246882263863212196902501646860688512623477701666912151330030589910588450063434942160882357865222421574524672916132175126793406560464293311434280830018165236526212633761186175831678733450736544345076166690510894491089448NCBITaxon:2759653552071089444655066897447094287535NCBITaxon:5833300692906526659581211776266595311786654913497675192964417725784544417711581419NCBITaxon:12066381666117467329073651668446896573960169944446859600NCBITaxon:58111182263477154119165784584840215530756636164034624136810893112686341428099612NCBITaxon:5820NCBITaxon:582126531155319921150857975522836801986373791966272905896059606446709603227086266596496045705091210454330905042929829387834810612793654483851074454191701134899615266599369169275736407821963257075298351036171206189257771285023308352701427869991417158779627300251102856312673615286964696439644441768746128113450696381715229965696577575011238693517469662227909637875767842181921195623305952167426979613443463601345196789676967712495153304394032246200966996858355476789689342054557600968110428039320111678078330381719117630247593217181717475933NCBITaxon:102981257118387279694836470384766438467635688NCBITaxon:150270298207621158612184512107496866111495720928520131913840405295071263854614666383379NCBITaxon:10239173610629943630870547515851744575412NCBITaxon:1024528586NCBITaxon:1024317481565633788067067135622583313618550992452779658334240906112563049495NCBITaxon:63121770707010094086929257074876138176514894232903217602399352146752947462947474376031781446560285321773846451772177437272557272559153481121532369014726647091317047258520920269785962859631263871214697212035332648373153214693583706903074937608333483333570472851111149708333283331214687108018749200637373505104287828014851750NCBITaxon:1155213992751562259311726231656777351116032932853422NCBITaxon:5759345089998103517307796407148457425457428121536024271875058NCBITaxon:104075678024271535242915954126291589203985781355886755122965879309458041920312410515358153985801331678906902725602725612214272563354422841027256434166379017904NCBITaxon:57963562355522221045775755969959263671101364CP010395.1224218573232239230741964164027848964472020693771756614403666610400540296543NCBITaxon:1170922469764992297936941638284593556182284590143673340353143673452763922611117957527640985986661362652810442179622490541565050176946996NCBITaxon:13131712507953389427955795942197228722854219253066129457041767240418891220889320889520755966452315217531536879830200465672139269635889NCBITaxon:13156730195388399227166665553256657123051120755534131359022491179986669400666684215767114353531151042983154689111298593273075660469136165105612149161522178523897633612149159704126308213288818831313613NCBITaxon:1865381889081916073NCBITaxon:1865366873039376527306855743615295266891057675358231223NCBITaxon:13830595984003585742917616089942917030027340542432327605681786064778161982159739975599802419947427577415593362371303681407340766670133888120410441111707111170829920407265636713914501344084408771117454797787454610740196794967957018640814082380394528135165966861235178616243231243230135992356127108978540964097978821035224327745157409097804092409344713529838615733178100816313625388654155401813416870436486979673239979371379548629916713033456433169173623125924851221282861109464722641988479451332103721284813173144077217913169315557781221119778219512012215225025518365231818600781131323848546456715916922591333499124961533454224327512047392432745365521340143649571657167849641673124966871601977162NCBITaxon:10357NCBITaxon:103581194881415784NCBITaxon:10359104585617072159765272634185934783411344582627626196947163495591364527262127262327262411607191238993121410240321714211821314403244903248343111477874909323848143711315368128664013684149185979949115437344030278083282352517429921895192952492949224924126267396731518766153348967301232801360319030414340119110224932493411020531979511211143919045186613907710619813530085592921486917198252157728141444818759449593625NCBITaxon:11676224135163434291104549526798953627290770590000-0001-8046-8102