Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Progenesisresults_WTvscebRmutant.csvftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Progenesisresults_cebRmutantvscebRmutantcellobiose.csvftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Progenesisresults_WTvscebRmutantcellobiose.csvftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Progenesisresults_WTvsWTcellobiose.csvftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/uniprot-streptomycesANDscabies.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies_cebRmutant_cellobiose_Bio1.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies_cebRmutant_Bio1.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies_cebRmutant_Bio3.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies8722_cellobiose_Bio1.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies_cebRmutant_cellobiose_Bio2.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies_cebRmutant_Bio2.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies8722_cellobiose_Bio3.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies_cebRmutant_cellobiose_Bio3.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies8722_cellobiose_Bio2.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies8722_cellobiose_Bio2.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies_cebRmutant_Bio2.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies_cebRmutant_Bio1.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies_cebRmutant_Bio3.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies8722_cellobiose_Bio3.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies8722_Bio2.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies8722_Bio1.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies8722_Bio3.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies8722_cellobiose_Bio1.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_QC.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies_cebRmutant_cellobiose_Bio3.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies_cebRmutant_cellobiose_Bio2.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies8722_Bio3.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies_cebRmutant_cellobiose_Bio1.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Raw_MSe_S.scabies8722_Bio2.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007820/Results_MSe_S.scabies8722_Bio1.zipprimaryOK200002360bart.devreese@ugent.beSören PlanckaertMass SpectrometryShotgun proteomicsNot availableLc-msePathogenicityStreptomyces scabiesSynapt mshttp://www.ebi.ac.uk/pride/archive/projects/PXD007820Proteins were extracted by sonication from Streptomyces scabies 87-22 and Streptomyces scabies Δscab57761 mycelium grown in the absence or presence of cellobiose for 48 hours in ISP-4 medium at 28°C. After TCA precipitation and Bradford analysis, the protein solutions (10 µg) were reduced (25 mM DTT, 10 min, 60°C), alkylated (75 mM IAA, 20 min, room temperature) and digested overnight with trypsin in 50 mM NH4HCO3 (37°C). The resulting peptides from each sample were separated on a NanoAcquity UPLC® system (Waters Corporation) in 2D mode (five fractions: 11.1%, 14.5%, 17.4%, 20.8%, and 45.0% of solvent B1). For the first dimension (high pH), solvent A1 and B1 were composed of 20 mM ammonium formate in water and ACN (pH 10), respectively. For the second dimension (low pH), solvent A2 and B2 were composed of 0.1% formic acid in water and 0.1% formic acid in ACN, respectively. Analysis of eluting tryptic peptides was performed using a Synapt G1 quadrupole time of flight mass spectrometer (Waters Corporation) equipped with a Nanolockspray source fitted with a pico-tip emitter. Accurate mass data were collected in a data independent positive mode of acquisition (MSe) by alternating between low and high-energy scan functions.PrideNot availableiodoacetamide derivatized residuemonohydroxylated residueThe acquired MSe data from three biological replicates of the four conditions were analysed using Progenesis LC-MS (v4.1, Nonlinear Dynamics). The retention times in each sample were aligned using a quality control sample to maximal overlay of the 2D feature maps. Protein identifications were obtained searching the Streptomyces scabies entries of a UniProt database using ProteinLynx GlobalSERVER version 2.5 (PLGS 2.5, Waters Corporation). The default protein identification criteria were used. Carbamidomethyl modification of cysteine (fixed) and methionine oxidation (variable) were selected as modifications. The PLGS results were imported into Progenesis LC-MS for annotation of peptide peaks. After normalization of the raw abundances of all features and statistical analysis, the different fractions were combined. Subsequently, protein abundances were compared using Progenesis statistic tools. ANOVA values of p < 0.05, regulation of 1.5-fold or 0.66-fold, power > 0.8 and q-values < 0.05 were regarded as significant for all further results.ProteomicsBart DevreeseSynapt MSPARTIALWE-10, L-ProBE, University Ghent, BelgiumStreptomyces Scabiei 87.2230229651 Planckaert S, Jourdan S, Francis IM, Deflandre B, Rigali S, Devreese B. Proteomic Response to Thaxtomin Phytotoxin Elicitor Cellobiose and to Deletion of Cellulose Utilization Regulator CebR in Streptomyces scabies. J Proteome Res. 2018 10.1021/acs.jproteome.8b00528soren.planckaert@ugent.beBiologicalUniversity Ghent, WE10, L-ProBEStreptomyces scabies is responsible for common scab disease on root and tuber vegetables. Production of its main phytotoxin thaxtomin A is triggered upon transport of cellulose byproducts cellotriose and cellobiose, which disable the repression of the thaxtomin biosynthesis activator gene txtR by the cellulose utilization regulator CebR. To assess the intracellular response under conditions where S. scabies develops a virulent behavior, we performed a comparative proteomic analysis of wild-type S. scabies 87-22 and its cebR null mutant (hyper-virulent phenotype) grown in the absence or presence of cellobiose. Our study revealed significant changes in abundance of proteins belonging to metabolic pathways known or predicted to be involved in pathogenicity of S. scabies. Among these, we identified proteins of the cello-oligosaccharide-mediated induction of thaxtomin production, the starch utilization system required for utilization of the carbohydrate stored in S. scabies's hosts, and siderophore synthesis utilization systems, which are key features of pathogens to acquire iron once they colonized the host. Thus, proteomic analysis supported by targeted mass spectrometry-based metabolite quantitative analysis revealed the central role of CebR as a regulator of virulence of S. scabies.Proteomic Response to Thaxtomin Phytotoxin Elicitor Cellobiose and to Deletion of Cellulose Utilization Regulator CebR in Streptomyces scabies.Planckaert Sören S, Jourdan Samuel S, Francis Isolde M IM, Deflandre Benoit B, Rigali Sébastien S, Devreese Bart Bsodium salt, Nup32D, BBE, ammonium formate, 13C-labeled, PLIP, cadmium salt, determination, immature single positive T cell, zinc formate, lead salt, protein, 2-(indol-3-yl)ethanoic acid, magnesium formate, composed of, zinc salt, Solvent, Type 2A-interacting protein, Long Term, peptide, Polypeptides, protein polypeptide chains, ammonium tetraformate, IAA, peptido, BANF, cobalt(II) formate dihydrate, dJ69E11.3, azanium, Min, Streptomyces scabies, protein aggregate, 4-O-beta-D-Glucopyranosyl-D-glucopyranose, present in fewer numbers in organism, Effect, DmelCG42273, HTATIP, nup154, CG11940, Divorced, me75, C79325, peptides, 2610036I19Rik, 2610510L13Rik, hypoplasia, min, mAPC, composition, proteins, Divorces, Myceliums, copper, BcDNA:LP01106, immature single positive T-lymphocyte, D17Mit170, T1, AI047805, Pico, fSAP152, decreased, lithium salt, ISP, cPLA2, ACN, sample, Acn, s, Acz, TIP, ammonium (2:1) salt, Long-Term Effects, Ammonium(1+), nickel salt, cobalt (+2) salt, ZC2HC5, Dmel_CG7826, Longterm Effect, HTATIP1, Tl3, tip, Tl2, ANAC091, Putative MAPK-activating protein PM10, NH4(+), (Indol-3-yl)acetate, sodium (4:1:1) salt, Dm1, magnesium salt, IES, TIP60, nickel (+2) salt, Dmel_CG7835, Hydrogen Oxide, Mnb, MNB, ammonium salt, absence, 4-O-beta-D-glucopyranosyl-, Skeletal muscle enolase, Sonications, content, formate, cobaltous formate, cromium (+3), AW124434, beta Trypsin, organ system, immature single positive T lymphocyte, Indole-3-acetic acid, heteroauxin, 4 O beta D Glucopyranosyl D glucopyranose, copper salt, ammonium (4:1) salt, Polypeptide, GSD13, lead (+2) salt, tlp, TIP41, time, Ammonium, nickel formate dihydrate, lead formate, Effects, aluminum salt, cesium salt, number, Eno-3, Gene, ACINUS, protein-containing complex, presence, body system, [NH4](+), CG7826, ESA1, formic acid, period, ammonium cation, potassium salt, polypeptide chain, reduced, subnumerary, 14C-labeled, Arabidopsis NAC domain containing protein 91, Gene Products, CG7835, CG42273, zk, system, Low, tiny, Separated, 4.2.1.11, Oospora scabies, l(2)10432, anatomical systems, Acinus, thallium (+1) salt, Longterm, ammonium ion, Actinomyces scabies, absent from organism, beta-Trypsin, rubidium salt, Indoleacetic acid, DmelCG11940, Long-Term, decreased number, calcium formate, methanoic acid, Solution, MSE, T.ISP.th, acinusL, DmelCG4579, ms(2)zk, (indol-3-yl)acetic acid, acinusS, peptidos, cromium (+3) salt, Long-Term Effect, l(2)01501, sodium formate, small, 1H-indol-3-ylacetic acid, mKIAA0559, DYRK1, data, cou, nickel formate, protein complex, BcDNA:LD21772, Proteins, 2-phospho-D-glycerate hydro-lyase, strontium formate, NH4+, potassium formate, copper (+2) salt, compositionality, strontium salt, Peptide, mKIAA0670, polypeptide, count in organism, Lr, native protein, Temperatures, natural protein, cupric formate, Protein, chemical analysis, Long Term Effects, Dyrk1, connected anatomical system, aluminum formate, AU020952, Separation, jog, nup32D, lithium formate, ammonium, underdeveloped, Separations, CC1, 3-Indolylessigsaeure, Tripcellim, CG4579, Muscle-specific enolase, sample population, chromic formate, Longterm Effects, ME-IV, Protein Gene Products, SCAN., Gene Proteins, Trypure, structure, D-Glucopyranose, Bra, calcium salt, TCV-interacting protein, Peptid, assay, quantitative, SCH, Enolase 3, presence or absence in organismOospora scabies, reactivity, Acid, Polyanhydroglucuronic, Heweten, Sulfite Cellulose, 4 O beta D Glucopyranosyl D glucopyranose, DEL, Polyanhydroglucuronic Acid, 4-O-beta-D-glucopyranosyl-, alpha-Cellulose, responsivity, Avicel, Rayophane, Actinomyces scabies., D-Glucopyranose, alpha Cellulose, Streptomyces scabies, response, regulator, 4-O-beta-D-Glucopyranosyl-D-glucopyranose, AlphacelBBE, Bru, results., Cysteine Hydrochloride, 2-amino-4-(methylsulfanyl)butanoic acid, Raw, Ass-1, L Isomer, Eno-3, protein, protein-containing complex, aligned, peptide, Polypeptides, protein polypeptide chains, L-Isomer Methionine, Methionine, ClvPrd, peptido, polypeptide chain, Non-linear Dynamics, AA408052, Plgs, L-Methionine, fold, Streptomyces scabies, Del(8)44H, Half-Cystine, protein aggregate, Models, Pedameth, 4.2.1.11, Oospora scabies, Svc, Chaos Theories, Theory, Non linear Dynamics, peptides, L Cysteine, M, Actinomyces scabies, proteins, MSE, retention, ASS, Nonlinear Dynamic, Quality Controls, 2-amino-4-(methylthio)butanoic acid, sample, peptidos, Non-linear Model, Theories, Half Cystine, Model, metionina, Racemethionine, statistical analysis, Chaos, data, Zinc Cysteinate, DL-Methionine, protein complex, AI447866, 2-Amino-4-(methylthio)butyric acid, 2-phospho-D-glycerate hydro-lyase, Maps, aligned to, Non-linear Models, L-Isomer, not genetically inherited, results, Peptide, Nonlinear, polypeptide, native protein, natural protein, Protein, Methionin, methionine, Hmet, Nonlinear Models, fixed, Non linear Models, Data Base, Met, Col4a-1, Non-linear, maintenance of localization, storage, Skeletal muscle enolase, Non-linear Dynamic, Chaos Theory, Control, Dynamics, Quality, L-Cysteine, Muscle-specific enolase, Controls, sample population, 1110030K05Rik, alpha-amino-gamma-methylmercaptobutyric acid, Peptid, regulation, GSD13, Polypeptide, sequestering, Nonlinear Model, variable, Liquimeth, Enolase 3other disease, d230, Sarcoptic, nucleocytoplasm, bioformation, Experiment., Pflanze, carbohydrate, number, saccharide, A4, dTAFII250, hydrates de carbone, biosynthesis, GRO:0005338, 2610511A05Rik, EfW1, glucide, presence, TYPE, viridiplantae, Alphacel, carbohydrates, BB234005, DAGA4, dmTAF[[II]]230, solute:solute exchange, exact), diseases, Polyanhydroglucuronic Acid, dmTAF1, Taf230, glucido, responsivity, disease or disorder, diseases and disorders, Streptomyces scabies, MAM, 4-O-beta-D-Glucopyranosyl-D-glucopyranose, SCG3, multicellular organismal biosynthetic process, TAF250, Oospora scabies, plantae, protoplasm, reactivity, Heweten, Taf200, single-organism biosynthetic process, human disease, Kohlenhydrate, dTAF[[II]]250, TFIID TAF250, protoplast, cel, formation, cell, anabolism, Actinomyces scabies, absent from organism, alpha Cellulose, Taf1p, behavioral response to stimulus, non-invasive carcinoma, non-neoplastic, synthesis, dTAF250, radix (exact), climbing root (narrow), in situ, Protein G18, disorder, Homo sapiens disease, carcinoma, TAF, behaviour, BACTS2, intracellular, single-organism behavior, carcinoma in situ, ahl4, radices (exact, dTAF[[II]]230, TAF[[II]]250, raíz (Spanish, alpha-Cellulose, Avicel, malignant, plural), Rayophane, disorders, TAF200, aerial root (narrow), l(3)84Ab, saccharides, medical condition, BG:DS00004.13, TAFII-250, TAF250/230, Cell, LGMD2C, all, dTAF230, Carbohydrates, count in organism, TAFII250, Experiment, single-organism transport, p230, CIS, Cis, thaxtomin A, Diseases, G18, TAF[[II]]250/230, condition, TFIID, 根 (Japanese, intraepithelial carcinoma, Sarcoptic Mange, internal to cell, glucides, Kohlenhydrat, saccharidum, Taf[[II]]250, Acid, Polyanhydroglucuronic, CIS-1, absence, TAF[[II]]230, DMDA1, 4-O-beta-D-glucopyranosyl-, Plant, common, 9030605P22Rik, TAF[II]250, root, stage 0 disease, CG17603, carbohidratos, Carbohydrate, TAF[[II]], disease, carbohidrato, DmelCG17603, DMDA, Sulfite Cellulose, behavioural response to stimulus, 4 O beta D Glucopyranosyl D glucopyranose, Taf250, SOCS, SR3-5, SCARMD2, D-Glucopyranose, small molecule transport, Mange, epithelial tumor, quantitative, response, tuber, glucidos, Suppressor of cytokine signaling, TAF230, TAF1, presence or absence in organismhost organism, nucleocytoplasm, determination, siderophore biosynthesis, A4, Amylum, Barretts Esophagus, Barrett's esophagus (disorder), hydrates de carbone, GRO:0005338, glucide, infectivity, Alphacel, carbohydrates, solute:solute exchange, primary metabolites, exact), diseases, Polyanhydroglucuronic Acid, Roles, responsivity, glucido, Concepts, diseases and disorders, Streptomyces scabies, 26Fe, Analysis, 4-O-beta-D-Glucopyranosyl-D-glucopyranose, Barrett's Syndrome, siderochrome biosynthetic process, multicellular organismal biosynthetic process, Barrett Epithelium, Mass Spectrum Analysis, BE, viral infection, Heweten, single-organism biosynthetic process, human disease, Kohlenhydrate, Analyses, anabolism, alpha Cellulose, proteins, virus process, behavioral response to stimulus, Barrett's esophagus, Barrett Esophagus, Vegetable, radix (exact), Starch, peptide modification, climbing root (narrow), Barrett Syndrome, Role Concepts, Homo sapiens disease, ulcerative esophagitis, intracellular, adenocarcinoma of esophagus, single-organism behavior, Barrett's ulcer of esophagus, Barrett's oesophagus with esophagitis, Process, columnar-lined esophagus, Avicel, plural), Rayophane, aerial root (narrow), Barrett's esophagus with esophagitis (disorder), saccharides, Barrett's esophagus (disorder) [Ambiguous], Spectrum Analysis, Acceptance Processes, predicted, Spectroscopy, Acceptance Process, Iron 56, Role Concept, siderochrome biosynthesis, single-organism transport, Epithelium, thaxtomin A, Diseases, Esophagus, columnar epithelial-lined Lower esophagus, Pathogenicity, Role, Barrett's oesophagus, 根 (Japanese, Sarcoptic Mange, internal to cell, regulator, Kohlenhydrat, Amylomaize, saccharidum, Acid, Polyanhydroglucuronic, absence, Iron, 4-O-beta-D-glucopyranosyl-, adenocarcinoma of oesophagus, Spectrometry, common, INSDC_feature:gene, root, siderophore anabolism, columnar epithelial-lined Lower oesophagus, Barrett's, siderophore formation, carbohidratos, siderophore biosynthetic process, Fe, organ system, Phenotypes, disease, carbohidrato, Eisen, DMDA, 4 O beta D Glucopyranosyl D glucopyranose, Barrett's esophagus with esophagitis, metabolites, cello, small molecule transport, siderophore synthesis, a carbohydrate, tuber, glucidos, iron, CLE, other disease, Sarcoptic, bioformation, (ulcerative esophagitis) or (Barrett's esophagus), Processes, Amylomaize Starch, carbohydrate, number, saccharide, secondary metabolites, Gene, biosynthesis, Spectrum Analyses, Cornstarch, presence, body system, TYPE, DAGA4, Gene Products, Mass, disease or disorder, system, MAM, SCG3, Barrett, Mass Spectroscopy, Oospora scabies, protoplasm, reactivity, study, peptide formation, hierro, anatomical systems, protoplast, Barrett esophagus, formation, Actinomyces scabies, absent from organism, non-neoplastic, synthesis, Barrett's ulcer of esophagus (disorder), Barrett metaplasia, columnar-lined oesophagus, Syndrome, Barretts syndrome, disorder, Behaviors, behaviour, radices (exact, raíz (Spanish, alpha-Cellulose, Proteins, disorders, fer, Barrett's ulcer of oesophagus, medical condition, LGMD2C, all, Concept, count in organism, Oligosaccharide, Keoflo, Protein, chemical analysis, proteomic analysis, condition, connected anatomical system, glucides, Mass Spectrum Analyses, Sarcoptic Mange., Acceptance, Mass Spectrum, ferrum, DMDA1, metabolite, Iron-56, Carbohydrate, Protein Gene Products, Gene Proteins, Sulfite Cellulose, behavioural response to stimulus, SCARMD2, D-Glucopyranose, Barrett esophagus/esophageal adenocarcinoma, Mange, virulence, assay, response, quantitative, presence or absence in organismOospora scabies, reactivity, Acid, Polyanhydroglucuronic, Heweten, Sulfite Cellulose, 4 O beta D Glucopyranosyl D glucopyranose, DEL, Polyanhydroglucuronic Acid, 4-O-beta-D-glucopyranosyl-, alpha-Cellulose, responsivity, Avicel, Rayophane, Actinomyces scabies., D-Glucopyranose, alpha Cellulose, Streptomyces scabies, response, regulator, 4-O-beta-D-Glucopyranosyl-D-glucopyranose, Alphacel236trueProteomic response to the thaxtomin phytotoxin elicitor cellobiose and to the deletion of the cellulose utilization regulator CebR in Streptomyces scabiesStreptomyces scabies is a plant pathogen responsible for common scab disease on root and tuber crops. The transport of the cellulose by-products cellobiose and cellotriose into the cell induces the biosynthesis of the main phytotoxin thaxtomin A. These carbohydrates bind to the cis-acting sequences of the cellulose utilization repressor CebR and thereby induce the expression of the thaxtomin activator TxtR. We performed a shotgun proteomic experiment to compare the wild-type S. scabies 87-22 and its cebR null mutant in the absence or presence of cellobiose. The global intracellular response during virulent behaviour of this plant pathogen could be determined during this experiment.2018-10-182017-09-22PXD0078202806551646099180498511145422492156843544071284218327042363336351198343006636362758385696363688881114301555711452631452623649346131269573666492391619364110665688951136364762723661112329442229555832323122586365434607925193659143205971293865581912502321195774818661968215867225680761755437359253668408169180454278775120108922505836741479564100392369420494334459741420012935293671567593643867716054048313152826930456271639162429204277944762376121810032962922113763948840479NCBITaxon:2157131527310510672059687201651636840717363096989361047821483643204210613204913816937432773204645351111032174592161292886712886764100694569410167634102107612773511316582135843014172981746336754128735675684030135062181301941119294393671104113644591394984320253012701047594120478861347495153721109716529982790513980317585439801260866398081375755518320196447111415542193255521138343911931819813981810269701069955529NCBITaxon:47518502983354764146547798251021699823897648407413735186287054824153NCBITaxon:50557984467525100036433414754803597413708115466547898353221599838106771034819852348780422531488414222863479461571871599191572952929211752719860148840633424951869123423515233598589859886559746499870392021110723635272159874190828743418235024145113969293190259390719017463601237474636172262536001319722119158531058859698951105198997237989772000263419610264266722758858156899628737562690724420237172019998570724074767927459615333554319313355418400513686150596866628666134751536329NCBITaxon:17632862873488376705802718481865368595986075105135294296110098402321000734882412336363071047005187878945556449447NCBITaxon:13356265419591399878168119011478281055524NCBITaxon:96061358584690081979153519307285930085290051184995262254121337591390143811139442370412044143708351581990119519532590841370137023943222774862615835583715371633376057267998086371137121906503714759172073127262981274575727090309031372814243371421044792491108239412NCBITaxon:961514780269266100658137352750454135663816475203750644223669502129349790546322455525365199764990814389923745374726473237602647312887055955362527403271602938511497864794362938247943722511775299167387193844137659393432715910022637674234442345467001287689591198467041464792936528034280382803510803481793922950271131681911265269971028621076015973611431140981087135547743658142450722953379698204232124223321803369822347081645733165467599287770203829314125295508114817448886437452649997283035718308550717629970767892683704151562887651388563924561152086595551655181135491432137159749535026143213858781707683194963401614280053164610466038151845474485009747697477118051240174841855601555479204274201488711053253297261005941289093164359900138943828354232423310829341220585604162991311227317018799152678181871049316407299467303337330485264638301130641399253064236121162065261610337286129940207340115357652611114716133865431201725323712552329935316317NCBITaxon:11084993841063267872862321933486208621160791392227681196125522814953932132232334774121827532917573129948861814084341033519963139248811533811533933043937556992862633911209488624157183997586444237486435332444260665332413439097682127178637863999839986865535291486541042986357358257632884019864982380102868899958665220341632138119380625631886571303443112130886581144889198466645463NCBITaxon:11072NCBITaxon:11071142800628835374375116597145943116598854331116781077854314408043933434NCBITaxon:11082693977382189931824431935128387145953116885311213278695berry86941003335869724436647015011142869215047514991054842047147251208470146497444974311700893062186441930611076323308056603960162179748258539460553965132127905688133775129817660171121478610502366946532051124501897441739412450128853183889198679488531911137910371647351660152943631631198573896939343237956026087121346334323223544314275241433611334565560291699636436802790105603140818838185733703401881274414437552162038713985584081724055986091838272759212744234081702759812744201274426235455382117627594328247480119915095648188229120455148167238473848190695148498101510693731263753405203860186497405224052141856451804NCBITaxon:813478009152275484793870333849917226175610946161435120325812055052352594473205493864651213880376686120678112202230741867051186705738733486912193879186802398007186801215451468351408186150847120677769183885160861138886915216778672127901008704351231719692812392948677133226126219813188268241122926571632957693812471246148545132838834839294911390363120416266589077151488003694169436945187901015080817053942065421055687317447127015329242814111946691169601454586953201019812646905505371408157697392830312383911283128212802811010832825253531522811235315349825741948153623142410145481NCBInr 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