Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM3_B.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM2_C.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM3_A.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM3_B.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM3_A.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM2_B.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM4_B.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM1_A.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM3_C.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM2_A.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM4_A.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM4_C.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM1_C.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM1_C.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM4_C.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM1_B.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM1_B.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM3_C.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM1_A.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM2_C.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM4_B.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM2_A.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM2_B.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM4_A.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM3_A_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM1_B_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM2_A_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM2_C_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM4_C_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM4_C_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM1_C_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM1_C_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM2_B_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM2_B_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM3_A_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM3_C_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM2_C_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM1_B_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM3_B_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM2_A_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM1_A_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM3_B_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM4_A_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM3_C_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM4_A_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM4_B_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM1_A_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM4_B_PEMMR.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM4_inj1uL_20131108_1_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM4_inj1uL_20131109_2_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM2_20131112_1_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM1_20131113_2_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM3_20131111_1_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM2_20131112_1_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM1_20131112_2_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM4_inj1uL_20131109_2_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM4_inj1uL_20131109_1_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM1_20131112_3_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM3_20131111_1_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM2_20131112_3_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM4_inj1uL_20131109_3_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM1_20131113_3_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM3_20131111_2_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM2_20131112_3_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM3_20131111_3_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM1_20131113_1_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM4_inj1uL_20131109_3_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM2_20131112_2_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM2_20131112_2_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM3_20131111_2_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_Ole_CM1_20131112_1_PEMMR.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD007906/PSY_NT_CM3_20131111_3_PEMMR.mgfprimaryOK200002820Ji-Hwan ParkNot availableCell CultureReagents and cell culture Sodium oleate (O7501) (P9767) was purchased from Sigma aldrich. Bovine serum albumin, fraction V, fatty acid-free (126575) was purchased from Calbiochem. Oleate was dissolved in 100% methanol. After conjugation with fatty acid-free BSA at 5:1 fatty acid to BSA molar ratio, it was diluted to proper final concentration in serum free Minimum Essential Medium (MEM) media just before the treatment to cells. HepG2 cells were grown in Minimum Essential Medium (MEM) (Welgene, LM 007-07) and then supplemented with 10% (v/v) fetal bovine serum (Lonza), 2 mM glutamine, 1% penicillin-streptomycin (Gibco) at 37°C, 5% CO2, and 95% humidity. Sample preparation HepG2 cells were starved for 12 hours before fatty acid treatment. To eliminate secretory factors from cells during starvation, cells were washed three times with 1x PBS. For oleate-treated samples, BSA-conjugated oleate diluted to the final concentration of 500 μM in serum free media was treated for 24 hours. For control samples, oleate-free serum free media containing equivalent amount of BSA and methanol to oleate-treated samples was treated for 24 hours. Conditioned media were prepared by replacing the media to unsupplemented serum free media and incubating for 24 hours in a 37°C CO2 incubator. After centrifuging the conditioned media at 3000 rpm for 10 min at 4°C, only supernatants were collected and filtered using centrifugal filter units (Millipore, UFC900324) to remove contaminants. Samples were dried by utilizing lysophilizer. Protein extraction and digestion The samples were dried and then homogenized with the lysis buffer [urea, NaCl, 50 mM Tris-HCl (pH 8.2) and a Complete Mini Protease inhibitor tablet (Roche Applied Science, Basel, Switzerland)]. The lysate was ultracentrifuged at 45,000 g at 4°C for an hour. Protein concentration was determined from the resulting supernatant by DC protein assay (Bio-Rad, Hercules, USA). The protein sample was then reduced in 0.5 M DTT (100 µl) for 50 min at 37°C, followed by addition of 1.5 µl of 1M iodoacetamide and incubation in the dark for 30 min at room temperature for alkylation. The resulting sample was subjected to in-solution tryptic digestion [1:50 enzyme-to-protein ratio (w/w), Promega, Madison, USA] and then incubated at 37°C overnight. The peptide samples were centrifuged at 2,500g for 10 min at room temperature. The resulting aqueous solution was desalted using solid-phase extraction with reverse-phase tC18 SepPak solid-phase extraction cartridges as previously described. Finally, the peptides were eluted with 50% ACN 0.5% HAcO and then dried in a Speed-Vac with resuspension in 0.1% formic acid. The samples were stored at -20°C before LC-MS/MS analysis. LC-MS/MS analysis All peptide samples were separated on a Thermo EASY-nLC 1000 (Thermo Scientific, Odense, Denmark) equipped with an analytical columns (Thermo Scientific, Easy-Column, 75 μm × 50 cm) and a trap columns (75 μm × 2 cm). The operation temperature of the analytical columns was at 50 °C. The flow rate was set to 300 nL/min. The solvent A and B were 0.1% formic acid and 2% acetonitrile in water and 0.1% formic acid and 2% water in acetonitrile, respectively. For the global proteome analysis, 120 min gradient (from 2% to 40% solvent B over 90 min, from 40% to 80% solvent B over 10 min, 80% solvent B for 10 min and from 80% to 2% solvent B over 10 min) was used. The eluted peptides from LC were analyzed using Q-Exactive™ hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific) equipped with the nanoelectrospray source. For the ionization of the eluting peptides, the electric potential of electrospray ionization was kept at 1.7 kV and the temperature of desolvation capillary was set to 270°C. Q-Exactive was operated in the data dependent mode with survey scans acquired for the mass range of 450-2000 Thomsons (Th) at a resolution of 70,000 (at m/z 200). Up to the top 10 most abundant ions from the survey scan were selected with an isolation window of 2.0 Th and fragmented by higher energy collisional dissociation (HCD) with normalized collision energies of 25 and the exclusion duration of 10 s. The MS/MS scans were acquired at a resolution of 17,500. Maximum ion injection times were 100 ms and 50 ms for full MS and MS/MS scan, respectively. Automated gain control (AGC) target value was set to 1.0 × 106 and 1.0 × 105 for full MS and MS/MS scan, respectively. In the submitted files, the label NT and Ole denotes "not treated" and "Oleate treaed", respectively. Also, CM1,2,3,4 represent 4 biological replicates, and A,B,C represent 3 technical replicates.PrideDaehee HwangQ ExactiveCenter for Plant Aging Research, Institute for Basic Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of KoreaCOMPLETE30076294 Park S, Park JH, Jung HJ, Jang JH, Ahn S, Kim Y, Suh PG, Chae S, Yoon JH, Ryu SH, Hwang D. A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cells. Exp Mol Med. 2018 50(8):93 10.1038/s12276-018-0120-3Korea Bioinformation Center (KOBIC), Korea Research Institute of Bioscience and Biotechnology (KRIBB)dhwang@dgist.ac.krMass SpectrometryShotgun proteomicsHumanHepg2Hepatocellular carcinomaOleatehttp://www.ebi.ac.uk/pride/archive/projects/PXD007906Not availableCarbamidomethylOxidationPeptide identification The fragmentation spectra were created based on mzXML file using msconvert (ProteoWizard release: 3.0.4323). MS data were first analyzed using postexperiment-monoisotopic mass refinement (PE-MMR) to assign accurate precursor mass to tandem MS data. MS/MS spectra were searched against a composite database containing UniProtKB/Swiss-Prot entries of the human reference proteome (UniProtKB release 2015_11, 42,123 entries) and 179 common contaminants in the target-decoy setting using MS-GF+ Beta (v10089) search engine under the following parameters: semi-tryptic, precursor mass tolerance of 10 ppm, carbamidomethylation of cysteine as a fixed modification and oxidation of methionine as a variable modification. The search results of 24 LC-MS/MS datasets were all combined, and the target-decoy analysis was performed on the combined dataset to obtain peptides with the false discovery rate (FDR) ≤ 0.01. Label-free peptide quantification To assign MS intensity to peptide identification, an MS intensity-based label-free quantification method was applied to 24 LC-MS/MS datasets. Briefly, through PE-MMR analysis, MS features of a peptide that appeared over a period of LC elution time in an LC-MS/MS experiment were grouped into a unique mass class (UMC). Ideally, each UMC corresponds to a peptide and contains the ions corresponding to the peptide, together with their mass spectral features, such as charge states, abundances (intensity), scan numbers, and measured monoisotopic masses. For each UMC, we obtained the UMC mass by calculating the intensity-weighted average of the monoisotopic masses of all the ions in the UMC. For the peptide corresponding to the UMC, its abundance was estimated as the summation of the abundances of all the ions in the UMC (UMC intensity). The refined precursor masses of the MS/MS spectra by PE-MMR were matched to the UMC masses and then were replaced with those of UMCs. In this process, MS/MS spectra information was linked to the matched UMC. The linked MS/MS spectra were assigned with a peptide sequence (peptide ID) with a FDR ≤0.01 after MS-GF+ searching and target-decoy analysis. The peptide ID was assigned to the UMC, and the UMC intensity was assigned to the peptide ID. Assignment of UMCs by master accurate mass and time tag database To assign the peptide IDs to unidentified UMCs, the master accurate mass and time tag (AMT) database (DB) was constructed and utilized as described previously. Briefly, the information about UMCs with peptide IDs from 24 LC-MS/MS data (triplicate LC-MS/MS experiments of 8 samples) was compiled into the master AMT-DB. The AMTs are unique peptide sequences whose monoisotopic masses and normalized elution times (NETs) are experimentally determined. For each AMT whose corresponding peptides were measured multiple times, the average mass and the median NET were recorded. We then mapped unidentified UMCs to AMTs in the master AMT DB with ±10 ppm of mass and ±0.02 of NET tolerances, and 3.0 of Excorr threshold. For each unidentified UMC matched to an AMT, the peptide ID for the AMT was assigned to the unidentified UMC, together with all information for the AMT (UMC mass, NET and Excorr). Alignment of the identified peptides After the assignment of unidentified UMCs using the master AMT DB, we combined the assigned and identified UMCs from LC-MS/MS datasets into an nm alignment table (peptide IDs for n UMCs and UMC intensities in m samples). The missing value in the alignment table means that the peptide was not identified in the corresponding datasets. For the missing values in each row of the table, we further searched for the UMCs that could be matched to the aligned UMCs based on their UMC masses and NETs with the following tolerance: ±10 ppm and ±0.01 across the technical replicates, and ±10 ppm and ±0.02 across the biological replicates, respectively. Quantile normalization was performed for UMC intensities in the alignment table to correct systematic variations of peptide abundances across datasets. To evaluate reproducibility of LC-MS/MS analysis, two types of similarity scores between LC-MS/MS datasets were calculated by measuring the overlap of detected peptides and their intensity values, as previously describedProteomicsHomo Sapiens (human)kp706@postech.ac.krBiologicalSouth Korea10.6019/PXD007906Increased fatty acid (FA) is often observed in highly proliferative tumors. FAs have been shown to modulate the secretion of proteins from tumor cells, contributing to tumor survival. However, the secreted factors affected by FA have not been systematically explored. Here, we found that treatment of oleate, a monounsaturated omega-9 FA, promoted the proliferation of HepG2 cells. To examine the secreted factors associated with oleate-induced cell proliferation, we performed a comprehensive secretome profiling of oleate-treated and untreated HepG2 cells. A comparison of the secretomes identified 349 differentially secreted proteins (DSPs; 145 upregulated and 192 downregulated) in oleate-treated samples, compared to untreated samples. The functional enrichment and network analyses of the DSPs revealed that the 145 upregulated secreted proteins by oleate treatment were mainly associated with cell proliferation-related processes, such as lipid metabolism, inflammatory response, and ER stress. Based on the network models of the DSPs, we selected six DSPs (MIF, THBS1, PDIA3, APOA1, FASN, and EEF2) that can represent such processes related to cell proliferation. Thus, our results provided a secretome profile indicative of an oleate-induced proliferation of HepG2 cells.A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cells.Park Soyeon S, Park Ji-Hwan JH, Jung Hee-Jung HJ, Jang Jin-Hyeok JH, Ahn Sanghyun S, Kim Younah Y, Suh Pann-Ghill PG, Chae Sehyun S, Yoon Jong Hyuk JH, Ryu Sung Ho SH, Hwang Daehee Dsodium salt, ammonium formate, 13C-labeled, cadmium salt, MeCN, ion, HepG2 cell, rad, Chiari malformation type 1 with syringomyelia, Basodexan, magnesium formate, Apaf-1, zinc salt, Solvent, RP11-508D10.1, 5730420M11Rik, dmTAF[[II]]230, Polypeptides, solid, oleic acid anion, protein polypeptide chains, L-glutamine, hydrogen chloride, cobalt(II) formate dihydrate, CH3-C#N, Urea, HCL-C, L-2-aminoglutaramic acid, Faroe Islands, WMS, C18:1 n-9, O-2-deoxy-2-(methylamino)-alpha-L-glucopyranosyl-(1-2)-O-5-deoxy-3-C-formyl-alpha-L-lyxofuranosyl-(1-4)-N, SET, Divorced, type 5 acid phosphatase, C79325, TFIID TAF250, sarcoma of breast, hac-1, cel, N'-bis(aminoiminomethyl)-, M, weight-weight percentage, phosphatase 2A inhibitor I2PP2A, Q, Oleic acid, proteins, Divorces, FA 18:1, AI047805, DmelCG4299, isolation and purification, salt, set, Strepto-Hefa, T-cell antigen Gp39, Wasserstoffchlorid, decreased, (9Z)-Octadecenoic acid, h, column, ACN, sample, Acn, OLEIC ACID, autolysin activity, TNFSF5, nickel salt, SGS, LC-MS2, dTAF[[II]]230, BZRP, Chp, cobalt (+2) salt, Tnfrsf5, anon-53Fa, TAF200, l(2)SH0173, Penicillin, breast sarcoma, not genetically inherited, PBS, hCD40L, ACMICD, PBR, common salt, sodium (4:1:1) salt, dens molaris, Dm1, magnesium salt, DmelCG42628, Oleat, Chlorwasserstoff, IMD3, Streptomycine Panpharma, Raps, leukemic reticuloendotheliosis, CG6829, Estreptomicina Normon, Hydrogen Oxide, dark/hac-1/dapaf-1, reticulate acropigmentation of Dohi, HLA-DR-associated protein II, DI-2, I-2Dm, proportionality, formate, NKTL, dyschromatosis symmetrica hereditaria 1, Dmel_CG15720, rate, cromium (+3), inhibitor of granzyme A-activated DNase, (S)-2, lysed material, w/w, I-2PP1, cis-Delta(9)-octadecenoic acid, Cm1, Painful bladder syndrome, TAF-IBETA, Taf250, spirit of wood, liquid chromatography-tandem mass spectroscopy, bacteriolytic toxin activity, ammonium (4:1) salt, TAF-Ibeta, methyl cyanide, chlorane, lead (+2) salt, Methyl Alcohol, TAF230, BPBS, GLUTAMINE, nickel formate dihydrate, lead formate, sarcoma of the breast, chlorure d'hydrogene, Ly62, fractured, TAPK, Streptomycin Grünenthal, aluminum salt, Levoglutamide, (9Z)-octadecenoate, dyschromatosis symmetrica hereditaria, CH3OH, Strepto Fatol, Carbamide, protein-containing complex, thomson, TR-AP, DmelCG5692, CG7826, mass-to-charge ratio, period, 18:1 n-9, isolation, LC-MSMS, GP39, CG7835, CG42273, Carmol, Oelsaeure, gp39, CD154, proportion, dTAF[[II]]250, GKLP, CATC4, pins, Acinus, Hydrogen chloride, thallium (+1) salt, cell, (2S)-2, rubidium salt, 2pp2a, Sciences, pk18, methanoic acid, ESI, ions, PINS, MBR, CG10574, Solution, acinusL, Injectable, dTAF250, PCTAIRE2-binding protein, Faeroe Islands, MGC:45012, hairy cell leukemia, 2PP2A, acinusS, Humidities, Kochsalz, dSET, dSet, PTBR, peptidos, Antibiotics, Hep-G2, dark/dapaf-1/hac-1, NTKL, mass percentage, Hep G2, CG5692, 1-(14)C-labeled, dApaf-1, split, ion(1-), Methyl alcohol, BG:DS00004.13, chloridohydrogen, potassium formate, DSH, DSH1, copper (+2) salt, ACP5, acetonitrile, buffer, SPENCDI, ribosome-associated ubiquitin-dependent protein breakdown, Cell, mKIAA0670, dTAF230, polypeptide, APAF1, native protein, Temperatures, Natriumchlorid, carbonyldiamide, Hac-1, I-2PP2A, cupric formate, chemical analysis, Dm I-2, hemorrhaged, TAF[[II]]250/230, Glutamic acid 5-amide, MFS1, Sodium, MeOH, NaCl, aluminum formate, Pins, Taf[[II]]250, AU020952, torn, (Z)-9-octadecenoic acid, lithium formate, holin, Glutamic acid amide, underdeveloped, Separations, Dark/Dapaf-1/HAC1, ethanenitrile, mDRC, ME-IV, (9Z)-octadec-9-enoate, quotient, CG4346, liquid chromatography tandem mass spectrometry, TRACP, 18:1Delta9cis, cis-9-octadecenoic acid, hydrochloric acid, Methoxide, liquid chromatography tandem mass spectroscopy, Hac-1/Dark, IPP2A2, Octadec-9-enoic acid, NCMe, determination, Blood, DmelCG6829, classic hairy cell leukaemia, zinc formate, lead salt, protein, Hydrogenchlorid, CG15720, Serum, Cahp, lysis, L-(+)-glutamine, Alkylations, peptide, Tnfsf5, Incubator, halite, ammonium tetraformate, L-Glutaminsaeure-5-amid, Streptomycin Sulfate, ClvPrd, peptido, BANF, Min, Apaf1, protein aggregate, present in fewer numbers in organism, ARK, Injectables, DmelCG42273, wood alcohol, Penicillin Antibiotics, T-BAM, rsh, LCMSMS, tartrate-resistant acid ATPase, reference sample, peptides, TAF-I, E927b, 2610036I19Rik, D-Glutamine, 2610510L13Rik, hypoplasia, min, arc, mAPC, IBP, purification, copper, ark, SCAN, free, T1, cloruro sodico, TRAP3, IGAAD, fSAP152, lithium salt, Tudor repeat associator with PCTAIRE-2, DmelCG10574, necrosis, dApaf1, blood capillary, Sodium Methoxide, Bladder pain syndrome, T5ap, MGC - 45012, GPHYSD2, ammonium (2:1) salt, Dmel_CG4346, ratio, symmetric dyschromatosis of the extremities, CD40L, phapii, cis-oleic acid, rock salt, Alcohol, D-Apaf-1, Molars, molar, LC-MS/MS, Cd40l, Dmel_CG7826, StF-IT-1, Th, Blood Serum, TAFII-250, classic hairy cell leukemia, TAF250/230, Thermo Scientific, fragmented, ur, Cm1 with syringomyelia., carbamide, TAFII250, HCD, Ionen, Streptomycin Sulphate, HCL, bacteriocin activity, p24, TNF-related activation protein, nickel (+2) salt, Carbinol, Ly-62, Dmel_CG7835, L Glutamine, carbinol, Mnb, MNB, ammonium salt, Painful Bladder Syndrome, ribosome-associated degradation, cracked, HCl, familial reticulate acropigmentation of Dohi, cobaltous formate, AW743063, ribosome-associated ubiquitin-dependent protein degradation, hac1, CG4299, MASS, HepG2, AW124434, AI326936, CG17603, natrii chloridum, Sid470p, TAF[[II]], REM3, L-glutamic acid gamma-amide, dapaf-1S, METHANOL, nanospray, Wood, H2NC(O)NH2, apaf-1, lysin activity, dapaf-1L, HEPG2, SR3-5, Acetamide, TrATPase, copper salt, IGM, CD40LG, Polypeptide, AI046351, RAD1, i2pp2a, 1728, Proteomes, time, (9Z)-octadec-9-enoic acid, Bladder Pain Syndrome, d230, cis-9-octadecenoate, Dapaf-1/HAC-1, TRAP, p50, 5-diamino-5-oxopentanoic acid, cesium salt, rapsyn, FBN, Methyl, dTAFII250, ACINUS, (2S)-2-amino-4-carbamoylbutanoic acid, vac, Methylalkohol, EfW1, Arnold-Chiari malformation type 1, PHAPII, LC-MS-MS, Buffer, formic acid, Dark/Hac-1/dApaf1, Karbamid, Hac1, (Z)-Octadec-9-enoic acid, potassium salt, Dark/Hac-1/dApaf-1, polypeptide chain, reduced, template-activating factor I, dmTAF1, Strepto-Fatol, Taf230, HEP-G2, subnumerary, ECTOL1, Injection, 14C-labeled, Harnstoff, Wood Alcohol, Strepto Hefa, Arnold-Chiari malformation type I, tiny, CD40-L, Separated, cloruro de hidrogeno, Pro-Mega, TAF250, Taf200, D-Streptamine, iones, ipp2a2, dapaf-1, chlorure de sodium, Taf1p, dapaf, methanol, dark, decreased number, calcium formate, OCTD, Estreptomicina CEPA, lysate, DARK, TEIF, wood naphtha, Trap, taf-ibeta, Chiari malformation type I, ribosome-associated ubiquitin-dependent protein catabolism, HIGM1, L-Glutamine, cromium (+3) salt, Chiari malformation type 1, TAF, dArk, Dapaf-1, Oleate, 2-iodo-, sodium formate, C78062, Controlled, small, P105, Controlling, DYRK1, data, TAF[[II]]250, CG42628, AA960066, 1500003O03Rik, nickel formate, DBI, 3H-labeled, protein complex, PCTAIRE2BP, igaad, strontium formate, total expressed protein, l(3)84Ab, apaf1, Bp50, L-2-Aminoglutaramic acid, acropigmentation of Dohi, strontium salt, Peptide, Methanol, [HCl], LC/MS/MS, capillary vessel, PKBS, natural protein, p230, Protein, I2PP2A, Hydrochloride, TFIID, table salt, Dyrk1, L-Glutamin, Dark, Dark/Apaf-I, ACETONITRILE, cyanomethane, WMS2, Separation, TAF[[II]]230, Streptomycin Sulfate (2:3) Salt, uree, urea, CC1, TAF[II]250, HEP G2, dApaf-1/DARK/HAC-1, sample population, chromic formate, UREA, sodium oleate, dSET/TAF-Ibeta, 2610030F17Rik, DmelCG17603, D Glutamine, Ion, concentration, Estreptomicina Clariana, velocity, wood spirit, SSKS, calcium salt, Peptid, RAD, Rad, assay, SCH, AA407739, Serums, dAPAF-1, TAF1, sodium chlorideProliferating, Octadec-9-enoic acid, d230, hepatoma, cis-9-octadecenoate, HepG2 cell, (9Z)-octadecenoate, dTAFII250, adult hepatoma, hepatoblastoma caused by somatic mutation, EfW1, hepatocellular carcinoma, dmTAF[[II]]230, oleic acid anion, (Z)-Octadec-9-enoic acid, 18:1 n-9, dmTAF1, HEP-G2, Taf230, liver cell cancer (hepatocellular carcinoma), Oelsaeure, TAF250, C18:1 n-9, Taf200, dTAF[[II]]250, TFIID TAF250, cel, cell, proliferating, adult primary hepatocellular carcinoma, Oleic acid, Taf1p, FA 18:1, dTAF250, (9Z)-Octadecenoic acid, hepatocellular, hepatoblastoma, OLEIC ACID, liver cell carcinoma, carcinoma, TAF, Oleate, Hep-G2, cis-oleic acid, dTAF[[II]]230, TAF[[II]]250, Hep G2, malignant, TAF200, ion(1-), carcinoma of liver cells, l(3)84Ab, BG:DS00004.13, TAFII-250, somatic, TAF250/230, Cell, dTAF230, hepatocellular adenocarcinoma, TAFII250, HCC, primary carcinoma of the liver cells, p230, Oleat, TAF[[II]]250/230, TFIID, carcinoma of the liver cells, hepatocellular cancer, Taf[[II]]250, (Z)-9-octadecenoic acid, liver and intrahepatic bile duct carcinoma, TAF[[II]]230, Hepatocellular carcinoma, primary carcinoma of liver cells, Hepatoma, childhood type, HEP G2, TAF[II]250, HepG2, CG17603, TAF[[II]], cis-Delta(9)-octadecenoic acid, DmelCG17603, (9Z)-octadec-9-enoate, TAF1., HEPG2, Taf250, Proliferation, SR3-5, carcinoma of liver, liver carcinoma, 18:1Delta9cis, cis-9-octadecenoic acid, cancer, liver cancer, (9Z)-octadec-9-enoic acid, TAF230liquid chromatography tandem mass spectroscopy, projections, ion, determination, NetrinA, D430049E23Rik, DELTA AND GAMMA, cd206, MS-GFDB, betaTub1, GCST, Long Term, peptide, Polypeptides, L-Isomer Methionine, B1t, Drug Tolerance, Methionine, ClvPrd, peptido, NKH, EG434437, Effect, WMS, average, Elkh, F, LCMSMS, kus, peptides, beta1tub, CG9277, M, EK6, T, beta-particle, Sap-2, Estimated, SCAN, free, neuroendocrine tumour, 1t, e, e-, Solute carrier family 6 member 2, beta1Tub, scientific observation, ppm, papilla, CT27014, NET1, SLC6A5, Half Cystine, netA, CD206, GPHYSD2, Racemethionine, Long-Term Effects, drug tolerance, SGS, ratio, LC-MS2, Elk, ELK, BETA 56D, anatomical protrusion, Data Set, PLATEST, Zinc Cysteinate, immune system tolerance, Neuronally-expressed EPH-related tyrosine kinase, T5E21.12, 2-Amino-4-(methylthio)butyric acid, LC-MS/MS, lamina, Longterm Effect, flanges, SAP2, Search, 9330129L11, Normalcy, Ids, results, neuroendocrine neoplasm, ACMICD, any method, l(1)G0108, Ionen, Norepinephrine transporter, shelf, Immunologic Tolerance, EPH-like kinase 6, MMR, FLOWERING TIME CONTROL PROTEIN FCA ALPHA, Methionin, overlap, median, methionine, DmelCG10226, Hmet, GCVT, T5E21_12, parent ion, long patch mismatch repair system, beta-tub, Beta <eudicots>, mismatch repair, shelves, proportionality, mmr, common, FCAALL.331, rate, L-Cysteine, MASS, beta56D, hEK6, DmelCG9277, projection, ridge, human, beta1-tub, beta(-), DTB2, beta-Tub, DmelCG18657, Health, Self Tolerance, precursor ion, Tolerance, GCE, spine, liquid chromatography-tandem mass spectroscopy, i6, DL4180C, Polypeptide, beta-Tub56D, Liquimeth, Proteomes, time, Platelets, betaTub, Specbeta, beta1-Tubulin, AW488255, MPS2, human being, Cysteine Hydrochloride, 2-amino-4-(methylsulfanyl)butanoic acid, False, Effects, lamellae, e(-), L Isomer, FBN, precursor, electron, aligned, process of organ, LC-MS-MS, protrusion, netrin, period, lamella, method, B-spec, Hek6, ECTOL1, betaspec, method used in an experiment, LC-MSMS, L-Methionine, Cek6, Half-Cystine, Pedameth, ENSMUSG00000074119, Normalities, Erp, APUDoma, ERP, Search Engines, SIDS, proportion, betatub(56D), MutS/MutL/MutH pathway, clec13d, Longterm, L Cysteine, iones, Elektron, beta[[1]] tubulin, Tyrosine-protein kinase receptor EPH-2, beta1t, ridges, Long-Term, ions, man, study assay, OCTD, anon-EST:fe1B3, Table, 2.7.10.1, 2-amino-4-(methylthio)butanoic acid, 10^[-6], SPEC8, Etrp, peptidos, Long-Term Effect, NAT1, CG5870, metionina, laminae, Net, NET, beta-tubulin56D, Spec-beta, measuring, data, Dmbeta1, C130099E04Rik, DL-Methionine, anatomical process, EPH tyrosine kinase 2, total expressed protein, Tubulin, aligned to, neuroendocrine tumor, i168, L-Isomer, net, Peptide, Engine, polypeptide, LC/MS/MS, Experiment, Immune Tolerance, AMT, chemical analysis, Long Term Effects, sequence, betaSpec, MFS1, b-Spec, flange, WMS2, Data Base, Met, organ process, DmelCG5870, l(1)G0074, l(1)G0198, negatron, Normality, beta[[1]]-tubulin, b spectrin, beta1, MutL-like pathway, CG18657, primary structure of sequence macromolecule, beta, Longterm Effects, Tub, plan specification, processes, process, Ion, beta-spec, EPHT2, SSKS, BETA, alpha-amino-gamma-methylmercaptobutyric acid, quotient, liquid chromatography tandem mass spectrometry, processus, Peptid, assay, netrin A, Peptide., Spec, Immunological Toleranceprojections, Octadec-9-enoic acid, MA387, ER60, Brp-14, HepG2 cell, neoplasia, insomnia (disease), Glycosylation-inhibiting factor, Tumor, Cell Growth in Number, dmTAF[[II]]230, oleic acid anion, GRP58, GRP57, MIF, Alp-1, Cell Number Growth, MIS, Grp58, Tumor Microenvironments, C18:1 n-9, LIAG, PLC[a], increased, Growth, inflammatory response, L1Ag, TFIID TAF250, cel, cell process disease, tumor disease, neoplasm (disease), Oleic acid, proteins, FA 18:1, Phenylpyruvate tautomerase, (9Z)-Octadecenoic acid, L1AG, MRP8, papilla, OLEIC ACID, P14, delayed sleep phase syndrome, Lvtw-1, tumor, associated, DER6, 60B8AG, apoA-I, Cancer Microenvironments, erp57, cis-oleic acid, dTAF[[II]]230, anatomical protrusion, TSP1, Apoa-1, lamina, TAF200, flanges, neoplastic disease, phenylpyruvate tautomerase, TAFII-250, MMIF, TAF250/230, results, Sep-2, L-dopachrome tautomerase, Sep-1, TAFII250, ER stress, CP-10, ERp61, Lipid, ERp60, shelf, Oleat, Cellular, ERp57, HsT17083, Glif, CGLB, CGLA, MRP14, shelves, susceptibility to, 5.3.3.12, PDI, tbsp1, HepG2, CG17603, TAF[[II]], projection, ridge, cis-Delta(9)-octadecenoic acid, DSPD, GLIF, Taf250, HEPG2, spine, Proliferation, SR3-5, insomnia, Cell Number, DSPS, P58, ApoA-I, other neoplasm, (9Z)-octadec-9-enoic acid, accessory, TAF230, Proliferating, d230, HEL-S-93n, cis-9-octadecenoate, Microenvironment, lamellae, (9Z)-octadecenoate, Gene, dTAFII250, apo-AI, EfW1, Delayed early response protein 6, supernumerary, process of organ, protrusion, lamella, delayed early response protein 6, (Z)-Octadec-9-enoic acid, HEL-S-269, dmTAF1, Cancer Microenvironment, 18:1 n-9, Taf230, HEP-G2, Metabolism, glycosylation-inhibiting factor, Gene Products, Glutathione-binding 13 kDa protein, Number Growth, neoplasm, Oelsaeure, TAF250, Erp, Taf200, THBS-1, dTAF[[II]]250, lipid metabolism, cell, 5.3.2.1, apolipoprotein A1, proliferating, L-dopachrome isomerase, Taf1p, inflammation, ridges, Plca, dTAF250, cell proliferation, time of survival, PI-PLC, Thbs-1, TAF, laminae, Oleate, Hep-G2, Cancer, Multiplication, TAF[[II]]250, Hep G2, Cellular Proliferation, anatomical process, Proteins, Sep2, l(3)84Ab, ion(1-), BG:DS00004.13, delayed sleep phase disorder, PDI-Q2, Cell, dTAF230, neoplastic growth, GIF, CAGB, survival, CAGA, grp58, APOA1, p230, Protein, TSP, TAF[[II]]250/230, TFIID, flange, organ process, 58kDa, Taf[[II]]250, (Z)-9-octadecenoic acid, TAF[[II]]230, increased number, TSP-1, TAF[II]250, HEP G2, CFAG, Ltw-1, disease of cellular proliferation, Protein Gene Products, present in greater numbers in organism, Gene Proteins, processes, process, THBS, DmelCG17603, (9Z)-octadec-9-enoate, SNAT, P8, TAF1., NIF, Cell Multiplication, processus, 18:1Delta9cis, cis-9-octadecenoic acid, Microenvironments, MAC387, TAF1projections, Octadec-9-enoic acid, MA387, Ef-2, ER60, Brp-14, HepG2 cell, neoplasia, Fasii, FasII, insomnia (disease), Glycosylation-inhibiting factor, FAS1, Tumor, FAS2, APO-1, Cell Growth in Number, dmTAF[[II]]230, DmelCG3665, oleic acid anion, GRP58, EF-2, EEF-2, GRP57, MIF, APO1, Alp-1, Cell Number Growth, FascII, fas2, SDR27X1, MIS, Grp58, C18:1 n-9, LIAG, PLC[a], increased, Growth, inflammatory response, L1Ag, TFIID TAF250, cel, cell process disease, tumor disease, neoplasm (disease), Bodily, Oleic acid, eef-2, proteins, FA 18:1, Phenylpyruvate tautomerase, Ab 1D4, 1D4, Akap550, FAS II, Secretion, (9Z)-Octadecenoic acid, L1AG, MRP8, FASD, Secretions, papilla, fas-II, OLEIC ACID, P14, delayed sleep phase syndrome, Lvtw-1, tumor, associated, DER6, 60B8AG, apoA-I, erp57, cis-oleic acid, dTAF[[II]]230, anatomical protrusion, TSP1, FASTM, Apoa-1, l(1)G0336, Tnfrsf6, external secretion, DmelCG4849, lamina, TAF200, CG3665, flanges, neoplastic disease, phenylpyruvate tautomerase, TAFII-250, MMIF, TAF250/230, results, exocrine gland fluid/secretion, Sep-2, Bodily Secretion, L-dopachrome tautomerase, Sep-1, TAFII250, ER stress, CP-10, ERp61, Lipid, ERp60, shelf, Oleat, fas II, Cellular, secretion, EG:EG0007.3, ERp57, HsT17083, Glif, CGLB, CGLA, mAb 1D4, MRP14, shelves, susceptibility to, APT1, 5.3.3.12, PDI, tbsp1, HepG2, OA-519, CG17603, TAF[[II]], projection, ridge, cis-Delta(9)-octadecenoic acid, DSPD, GLIF, Taf250, HEPG2, spine, SR3-5, Proliferation, insomnia, Cell Number, EF-2b, DSPS, P58, TNFR6, ApoA-I, other neoplasm, FAS, Fas, exocrine gland secretion, (9Z)-octadec-9-enoic acid, accessory, TAF230, Proliferating, Fas 2, eEF2, eef2, d230, HEL-S-93n, anon-EST:Liang-1.60, cis-9-octadecenoate, exocrine gland fluid, exocrine gland fluid or secretion, lamellae, EFTUD2, Gene, (9Z)-octadecenoate, dTAFII250, apo-AI, fas, TNFRSF6, EfW1, Delayed early response protein 6, supernumerary, process of organ, DmelCG6775, protrusion, ALPS1A, CD95, lamella, delayed early response protein 6, (Z)-Octadec-9-enoic acid, HEL-S-269, dmTAF1, 18:1 n-9, Taf230, HEP-G2, Metabolism, glycosylation-inhibiting factor, Gene Products, Glutathione-binding 13 kDa protein, anon-EST:Liang-1.44, Number Growth, neoplasm, Oelsaeure, TAF250, Erp, Taf200, SCA26, THBS-1, l(1)G0032, dTAF[[II]]250, lipid metabolism, clone 1.44, cell, 5.3.2.1, apolipoprotein A1, proliferating, L-dopachrome isomerase, Taf1p, inflammation, ridges, CG15466, CG2238, Plca, dTAF250, l(1)G0048, secreted substance, MAB1D4, cell proliferation, FAS 11, bodily secretion, time of survival, EEF2, PI-PLC, lpr, clone 1.60, Thbs-1, TAF, laminae, Oleate, Hep-G2, CT12301, AI196731, Multiplication, TAF[[II]]250, Hep G2, Cellular Proliferation, anatomical process, Proteins, Ef2, Sep2, l(3)84Ab, ion(1-), CG6775, BG:DS00004.13, delayed sleep phase disorder, PDI-Q2, l(1)G0293, Cell, dTAF230, neoplastic growth, GIF, CAGB, survival, CAGA, grp58, APOA1, DAKAP550, p230, Protein, TSP, EF2, ef2, TAF[[II]]250/230, TFIID, Fas II, flange, organ process, 58kDa, Taf[[II]]250, (Z)-9-octadecenoic acid, AKAP, TAF[[II]]230, increased number, eF2, TSP-1, TAF[II]250, HEP G2, CFAG, Ltw-1, disease of cellular proliferation, A630082H08Rik, Protein Gene Products, mAB1D4, FASII, present in greater numbers in organism, Fetal Alcohol Syndrome, Gene Proteins, processes, process, THBS, DmelCG17603, (9Z)-octadec-9-enoate, SNAT, P8, DmelCG2238, TAF1., NIF, Cell Multiplication, mAb1D4, processus, fasII, 18:1Delta9cis, l(1)G0081, cis-9-octadecenoic acid, MAC387, TAF1C18:1 n-9, Proliferating, (Z)-9-octadecenoic acid, cis-oleic acid, Octadec-9-enoic acid, hepatic cancer cell line, Hep G2, cis-9-octadecenoate, hepatocellular carcinoma cell line., hepatocarcinoma cell line, HepG2 cell, proliferating, ion(1-), (9Z)-octadecenoate, Oleic acid, HEP G2, liver cancer cell line, HepG2, FA 18:1, cis-Delta(9)-octadecenoic acid, oleic acid anion, (Z)-Octadec-9-enoic acid, (9Z)-Octadecenoic acid, (9Z)-octadec-9-enoate, HEPG2, 18:1 n-9, HCC cell line, Proliferation, HEP-G2, Oleat, OLEIC ACID, 18:1Delta9cis, cis-9-octadecenoic acid, Oleate, Hep-G2, (9Z)-octadec-9-enoic acid, Oelsaeure282trueA secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cell lineIncreased fatty acid (FA) is often observed in highly proliferative tumors, contributing to promoting proliferation of tumor cells. FA affects secreted factors from tumor cells, which can modulate tumor microenvironment in favor of tumor survival. However, the secreted factors affected by the increased FA have not been systematically explored. Here, we performed comprehensive secretome profiling of oleate-treated and untreated HepG2 cells. Comparison of the secretomes identified 349 differentially secreted proteins (DSPs; 145 up-regulated and 192 down-regulated) in oleate-treated samples, compared to untreated samples. Functional enrichment and network analyses of the DSPs revealed that the 145 up-regulated secreted proteins by oleate treatment were mainly associated with cell proliferation-related processes, such as lipid metabolism, inflammatory response, and ER stress. Based on the network models of the DSPs, we selected four up-regulated secreted proteins (MIF, THBS1, PDIA3, and APOA1) that can represent such processes related to cell proliferation. Thus, our results provided a secretome profile indicative of oleate-induced proliferation of HepG2 cells.2018-08-082017-10-04PXD007906280655164609918049829535851114514621333524422492156843179819354407110946194202452787284218809036809327042933084581058113633363515242601198343006636362758385696363NCBITaxon:128068888561896111430118726351472555711452631452623649346136403195206512695736664923916197467073853883641135461582158231066590341688953905311363647627236611123294422291180995558323231225863654583334607925193659143205971293865581958511493152425911308322637371250232NCBITaxon:1272111957748186619682114885843104300245135867225680761755437359253668408169180454266834295319573826453058612668352787751201089149822505814963674452158554528113208147956410039237514636942049433208964379504208963445974137979138712728384400667142001220327545326029352936447106715675938803647843464386771605216599404832165972165954550323098131528221659669304216591562716169683391624314145321832175462712920427794476237645431218100172030949801945477525556978159509329629439843948840479NCBITaxon:215752533813152735515310510674398990371228577595152059687622623112108862445555888201651645587077918178081736309431796758144571698936160488409859NCBITaxon:371621047826794015059071483643204210613204913816936307432773555432046632633311994565456745351102541110321745921612928867126420128867641002642036945694101676341021076127735152486769674913165823925472793342814613584301417298760917463345774449167547604893212873567568403013506219172652579250181301941119294393671104113644591673428102393202555986325830127027883965910475941203474951537211097160490165299827905133552527313366366666810242980366917585439801102432608663102459808137577574175551832019644714526801267577764145796631367019083233115618089581141553551067642193255521138343965719276349813981810269701235987106995552944456220668350701NCBITaxon:47518502983354761136157414654771800665564843376776020721441379299767982510216998238976414008208407414008211373511557186287011089725482444474153NCBITaxon:5055789969844675251000364653491486246334147147493548019292951946751299114359741276473137081154666326547876565893639835322159589366983819201110677577271034811208370985234878042253265554148841422286380966409861479464098609958615718717668599196337157295137221171101292921175271107687834974198602028091348585134858450253924991488406334249518691234235152335985898591086198865591324974649987039202111072943311711393635272159874190850371170827117082828743418211445223502414511396929319021978224593907190174636046147123747226940210195375939723287581721874202532566481219071NCBITaxon:573905079452659600131972211915853101774208588596321387NCBITaxon:615750521028307989515586411051989972375059989712466635761317200026318876341961026426610888781761792121906594071219067722758858112111045689964975151950582873756269072445066NCBITaxon:250682023717NCBITaxon:12065118703612201999857063446950605061724074767927450627249159615333554319313355418400524643213686150596487844612286662941786661347515363291018194301086731018510184NCBITaxon:176357303963558633149246437286287151458348837670580430801321650852718481865368595105135553482260710185687294296110091416333NCBITaxon:619184023210007348824123363630711218771047005812718787864515981289455564742163577553470951451225786425011449447260704NCBITaxon:1335626260705260707573061658457367734054163677483167233150561007133705654195NCBITaxon:3055504457913998781681190119287512987222829126806315327341478281055524NCBITaxon:9606135858469008171929111167815021979153283051930NCBITaxon:208964283672859300852900561742NCBITaxon:569382688306401794699138995915101184995262254121334352587591315159014140129448242838111394422584416041937047592721204414370860423281677285035158199011912031051953259084126738637013702492405360883858115589227054156224883943229365340367718615622774881756261583558371537163337605726711935015075229980861193502167879108579137113712724071906503714759171329121226620731272629812880571256274575727013290903090311333550372814243371421043857917851347924910078752824NCBITaxon:610111082NCBITaxon:961572433594633185862607991612432141262200298147802492670NCBITaxon:1263719281249212528303856838569591169266100658137353096322375612750454266625515197881356638164752037506442236695023600941293497146042290546322455525363524721401257515115199764990851515143899237454617037474454464152264732432359376026473159202228870515805955361001999555088460625274015793271602938515781928650114978633892479436293821162089453593647943750917389440NCBITaxon:6854412754225117752991590109013416738715895521012966103061296714236871585158451584915759219384413765939341410384327159192864810022637671928649369113388914792359046142344423454670012876895911983329661091494467041464792936576164280341160221641331599280382803536909108917688149229990894621812141080348179392464028788929502711313329528282944568617493468191126526997227321890382102862107601247190400727NCBITaxon:991315973699782811431140206411981087135547743658142450722953379698204232124223129163218033698223470871453720916457332153581575462669402656194013716546759928777020206032811431933829314125295508332912924129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