Pride

application/xml

ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_3a.mzMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_2a.mzMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_1a.mzMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/QE_elastase_Evasin_degly-1.mzMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_1a.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_2a.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_3a.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/QE_elastase_Evasin_degly-1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_2a.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_1a.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/QE_elastase_Evasin_degly-1.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_3a.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_3a.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_1a.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/170703_Evasin_degly_1-50_2a.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008025/QE_elastase_Evasin_degly-(1).pride.mgf.gzprimaryOK200sbhattac@well.ox.ac.ukLucia Geis-AsteggianteMass SpectrometryShotgun proteomicsRhipu-p672Glycosylation siteshttps://www.ebi.ac.uk/pride/archive/projects/PXD008025P672_RHIPU was deglycosylated using PNGaseF and Endo-F1. The deglycosylated P672_RHIPU was reduced with dithiothreitol, alkylated using iodoacetamide and digested with trypsin or neutrophil elastase. Peptide aliquots from tryptic digests (n=3) were analysed by LC-MS/MS using an Easy-nLC 1000 coupled to an Orbitrap-LTQ-XL (Thermo Fisher Scientific). Peptides were desalted and concentrated by a C18 PepMap 100 trap (300um x 5mm, 5um particle size, 100 Å) and separated by a C18 Acclaim PepMap RSLC column (75 um x 250 mm, 2um particle size, 100Å) using a linear gradient from 0 to 80% acetonitrile:H2O with 0.1% formic acid during 25 min at a flow rate of 300 nL/min. For samples run in the orbitrap-LTQ-XL, precursor ions were acquired at a mass resolution of 60,000 (m/z 400) and product ions were acquired in the ion trap. The 5 most intense precursor ions of charge ≥2 were selected for fragmentation by collision induced dissociation (35V) and dynamic exclusion settings allowed a precursor to be selected up to 3 times in a period of 30s prior to be excluded for 60s. A peptide aliquot (n=1) from an elastase digest was analysed using a Q Exactive mass spectrometer coupled with a Dionex Ultimate 3000 UPLC. Samples were desalted online (PepMap C18, 300µm x5mm, 5 µm particle size) for 1 minute at a flow rate of 20 µl/min and separated on a nEASY column (PepMap C18, 75 µm x 500mm, 2 µm particle size) over 60 Minutes using a gradient of 2%-35% acetonitrile in 5% DMSO/0.1% Formic acid at a flow rate of 250nL/min. Survey scans were acquired at a resolution of 70,000 @ 200m/z, and the 15 most abundant precursors of charge between 2 and 7 were selected for HCD fragmentation (35V) and dynamic exclusion parameters were set in order to select a precursor ion once prior to be excluded for 60s.PrideNot availableData files were analysed using Proteome Discoverer 2.2 (Thermo Fisher Scientific) with the search engine SEQUEST HT against a custom-made sequence database including RHIPU-P672 protein sequence and a list of typical contaminant proteins (246 entries). Precursor and product ion tolerances were set to 20 ppm and 0.6 Da and up to 2 missed cleavages were allowed. Carbamidomethylation was required as a static modification, and deamidation of asparagine present in N-X-S/T motif, oxidation of methionine, and HexNAc in Asn residues were considered as variable modifications. The presence of 2 unique peptides with false discovery rate FDR ≤ 1% and PTM site probability threshold ≥ 75 were required for identification and modification site location. Manual inspection of precursor and product ion spectra was performed in order to confirm the sequence fragmentation patterns observed.ProteomicsShoumo BhattacharyaWellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.COMPLETERhipicephalus Pulchelluslucia.geisasteggiante@chem.ox.ac.uk29487134 Eaton JRO, Alenazi Y, Singh K, Davies G, Geis-Asteggiante L, Kessler B, Robinson CV, Kawamura A, Bhattacharya S. The N-terminal domain of a tick evasin is critical for chemokine binding and neutralization and confers specific binding activity to other evasins. J Biol Chem. 2018 10.1074/jbc.ra117.000487BiologicalUniversity of OxfordUnited Kingdom10.6019/PXD008025Tick chemokine-binding proteins (evasins) are an emerging class of biologicals that target multiple chemokines and show anti-inflammatory activities in preclinical disease models. Using yeast surface display, we identified a CCL8-binding evasin, P672, from the tick Rhipicephalus pulchellus We found that P672 binds CCL8 and eight other CC-class chemokines with a Kd < 10 nm and four other CC chemokines with a Kd between 10 and 100 nm and neutralizes CCL3, CCL3L1, and CCL8 with an IC50 < 10 nm The CC chemokine-binding profile was distinct from that of evasin 1 (EVA1), which does not bind CCL8. We also show that P672's binding activity can be markedly modulated by the location of a StrepII-His purification tag. Combining native MS and bottom-up proteomics, we further demonstrated that P672 is glycosylated and forms a 1:1 complex with CCL8, disrupting CCL8 homodimerization. Homology modeling of P672 using the crystal structure of the EVA1 and CCL3 complex as template suggested that 44 N-terminal residues of P672 form most of the contacts with CCL8. Replacing the 29 N-terminal residues of EVA1 with the 44 N-terminal residues of P672 enabled this hybrid evasin to bind and neutralize CCL8, indicating that the CCL8-binding properties of P672 reside, in part, in its N-terminal residues. This study shows that the function of certain tick evasins can be manipulated simply by adding a tag. We conclude that homology modeling helps identify regions with transportable chemokine-binding functions within evasins, which can be used to construct hybrid evasins with altered properties.The N-terminal domain of a tick evasin is critical for chemokine binding and neutralization and confers specific binding activity to other evasins.Eaton James R O JRO, Alenazi Yara Y, Singh Kamayani K, Davies Graham G, Geis-Asteggiante Lucia L, Kessler Benedikt B, Robinson Carol V CV, Kawamura Akane A, Bhattacharya Shoumo SO-linked Glycosylations, GlcNAcylation, Protein Glycosylation, Protein Glycosylations, Galactosylation, N linked Glycosylation, N-Acetylglucosaminylation, Sialylations, N-linked Glycosylation., Galactosylations, Glycosylations, N-linked Glycosylations, Sialylation, Fucosylations, O linked Glycosylation, N-linked, O-linked Glycosylation, glycosylation, Glycosylation, Phosphoglycosylations, N Acetylglucosaminylation, Phosphoglycosylation, O-linked, Protein, GlcNAcylations, Fucosylation, N-AcetylglucosaminylationsIPP2A2, posttranslational modification, 2-amino-4-(methylsulfanyl)butanoic acid, False, number, L Isomer, Gene, Hasp, protein, precursor, protein-containing complex, presence, PHAPII, 5730420M11Rik, Polypeptides, L-Isomer Methionine, Methionine, peptido, Gene Products, L-Methionine, 2, protein aggregate, placement, Pedameth, DL-Asparagine, Search Engines, SET, posttranslational amino acid modification, asparagina, F, peptides, TAF-I, M, N, L-Asparagine, ipp2a2, 2pp2a, posttranslational protein modification, ASN, CG10574, Asn, Probabilities, DmelCG4299, Asparagin, IGAAD, set, 2PP2A, 2-amino-4-(methylthio)butanoic acid, 10^[-6], DmelCG10574, taf-ibeta, ppm, dSET, dSet, site, peptidos, metionina, Racemethionine, phapii, DL-Methionine, protein complex, Proteins, igaad, 2-Amino-4-(methylthio)butyric acid, StF-IT-1, Search, L-Isomer, Peptide, Engine, group, count in organism, native protein, I-2PP2A, Protein, Dm I-2, I2PP2A, sequence, Methionin, relational spatial quality, Hmet, region, Data Base, Met, parent ion, HLA-DR-associated protein II, PTM, ensemble, DI-2, I-2Dm, CG4299, 2-amino-3-carbamoylpropanoic acid, 4-diamino-4-oxobutanoic acid, primary structure of sequence macromolecule, post-translational amino acid modification, Protein Gene Products, I-2PP1, Gene Proteins, dSET/TAF-Ibeta, 2610030F17Rik, TAF-IBETA, precursor ion, post-translational modification, alpha-amino-gamma-methylmercaptobutyric acid, primary structure of sequence macromolecule., Peptid, TAF-Ibeta, Polypeptide, variable, AA407739, i2pp2a, Liquimeth, locationGeneral activity., Activity, neutralization |biological activity, ligandliquid chromatography tandem mass spectroscopy, sodium salt, Methane, Rps17, IPP2A2, ammonium formate, Size, 13C-labeled, Granulocyte, MeCN, cadmium salt, precursor., NCMe, ion, Lysosomal Elastase, Particle, zinc formate, RPS17, BOUND WATER, lead salt, magnesium formate, zinc salt, heavy chain disease, RP11-508D10.1, Polymorphonuclear, HOH, 5730420M11Rik, peptide, M(3)67, Polypeptides, Tnfsf5, M(3L)i, ammonium tetraformate, peptido, cobalt(II) formate dihydrate, CH3-C#N, B1, 1, 2, Min, CD105, Rheumabene, DmelCG42273, (R*, T-BAM, SET, type 5 acid phosphatase, Divorced, LCMSMS, tartrate-resistant acid ATPase, peptides, TAF-I, M, l(3)67BDo, hypoplasia, dimethyl sulphoxide, min, mAPC, Divorces, copper, AI047805, DmelCG4299, Polymorphonuclear Leukocyte Elastase, TRAP3, IGAAD, set, T-cell antigen Gp39, Tudor repeat associator with PCTAIRE-2, lithium salt, column, Dimethylsulfoxide, Sputolysin, DmelCG10574, D1, D-endo, dihydridooxygen, S-endoglin, TNFSF5, D9, T5ap, dihydrogen oxide, MGC - 45012, M(3)RpS17, ammonium (2:1) salt, Dimethylsulfoxid, nickel salt, AI662476, LC-MS2, CD40L, phapii, sulfinylbis-, DMSO, Granulocyte Elastase, dimethyli sulfoxidum, dmso, cobalt (+2) salt, Tnfrsf5, methylsulfinylmethane, LC-MS/MS, Cd40l, aqua, Dmel_CG7826, M(3)67C, StF-IT-1, Th, 4-dimercapto-2, not genetically inherited, hCD40L, Menstruation, H2O, (CH3)2SO, sodium (4:1:1) salt, elastase-2, HCD, Cleland, magnesium salt, Dm1, Ionen, M(3)i(55), D-endoA, IMD3, Sizes, TNF-related activation protein, nickel (+2) salt, Sulfinylbis(methane), Ly-62, endomannosidase, Dmel_CG7835, Rimso 50, Neutrophil Elastase, Mnb, MNB, parent ion, rpS17, ammonium salt, HLA-DR-associated protein II, DI-2, Rimso50, I-2Dm, Rp S17, NKTL, formate, hydrogen hydroxide, cobaltous formate, AW743063, cromium (+3), 3-Butanediol, CG4299, ELA2, AI326936, AW124434, Elastase, endo, beta Trypsin, acqua, I-2PP1, R*)-1, TAF-IBETA, precursor ion, Acetamide, liquid chromatography-tandem mass spectroscopy, TrATPase, IGM, copper salt, DmelCG14296, ammonium (4:1) salt, CD40LG, TAF-Ibeta, Polypeptide, Wasser, methyl cyanide, lead (+2) salt, 4-Dimercapto-2, i2pp2a, Dimethyl, Cleland Reagent, Particle Sizes, rac-Dithiothreitol, nickel formate dihydrate, TRAP, p50, lead formate, threo-1, MANEA, Reagent, Ly62, Cleland's reagent, TAPK, aluminum salt, cesium salt, 4-dimercapto-, dimethylsulfoxyde, Rimso 100, precursor, R*)-, thomson, TR-AP, CG7826, PHAPII, LC-MS-MS, agua, formic acid, mass-to-charge ratio, Sulphoxide, potassium salt, M(3)S33, M(3)i, reduced, IT, 14C-labeled, LC-MSMS, GP39, CG7835, CG42273, Endo, tiny, CD40-L, Separated, gp39, S(O)Me2, CD154, sulfinylbis(methane), CATC4, GKLP, thallium (+1) salt, Lysosomal, iones, ipp2a2, beta-Trypsin, rubidium salt, Dendo-A, Minute, 2pp2a, eau, calcium formate, methanoic acid, l(3)dtOA4, ions, CG10574, Rimso-50, AI528660, dimetil sulfoxido, PCTAIRE2-binding protein, MGC:45012, Rimso, Dimethylsulphinyl, 2PP2A, DmelCG3922, TEIF, Trap, taf-ibeta, M(3)i[55], dSET, dSet, HIGM1, peptidos, cromium (+3) salt, Neutrophil, 2-iodo-, sodium formate, PTHB1, C78062, small, 3-butanediol, P105, NTKL, DYRK1, Leukocyte, 1-(14)C-labeled, nickel formate, 3H-labeled, PCTAIRE2BP, igaad, endo-alpha mannosidase, Clelands Reagent, strontium formate, Bp50, potassium formate, copper (+2) salt, PMN Elastase, ACP5, acetonitrile, strontium salt, SPENCDI, Dithiothreitol, Polymorphonuclear Leukocyte, Peptide, group, Dithiotreitol, polypeptide, LC/MS/MS, Endometriosis, Period, Dimexide, I-2PP2A, C18, cupric formate, Dimethylsulphoxide, Dm I-2, DL-threo-1, I2PP2A, BcDNA:RE44119, Dyrk1, Sulfoxide, aluminum formate, ACETONITRILE, DTL, cyanomethane, Cleland's, AU020952, Separation, DTT, lithium formate, PRSS, underdeveloped, ensemble, Separations, CC1, M(3)q, Tripcellim, dimethyl sulfoxide, Sclerosol, Dimethyl Sulphoxide, ethanenitrile, CG14296, anon-EST:Posey48, chromic formate, ME-IV, CG3922, Cleland's Reagent, Trypure, dSET/TAF-Ibeta, mandaselin, 2610030F17Rik, Ion, PMN, calcium salt, M(3L)i[55], liquid chromatography tandem mass spectrometry, Peptid, TRACP, [OH2], dimethyl sulfur oxide, AA407739, Leukocyte ElastaseAT1G11140, Forms, other disease, Chemotactic, MIP-1a, Activity, conformation, Peptidomics, SCYA10, Chemotactic Cytokines, D17S1718, Gene, baker's yeast, LD78alpha, G0S19-2, Saccharomyces italicus, G0S19-1, MIP-1alpha, CG12298, SUB, surface, diseases, DmelCG12298, isolation, yeast, Chemokine, Gene Products, disease or disorder, diseases and disorders, SCRAMBLED, beta-Chemokine, C-C, Chemotactic Cytokine, KIF20A, MIP1A, placement, lager beer yeast, integumentum commune, MIP-1-alpha, LD78-beta(1-70), study, human disease, ligand, mei-1794, SCYA8, MIP1-alpha, Saccharomyes cerevisiae, purification, Saccharomyces uvarum var. melibiosus, LD78ALPHA, beta-Chemokines, non-neoplastic, CC, isolation and purification, Candida robusta, C-C Chemokines, Saccharomyces capensis, SCYA3, MIP1-(a), disorder, Homo sapiens disease, SRF9, brewer's yeast, LD78, Scya3, TAG, SCYA3L1, HC14, distinct from, AI323804, C-C Chemokine, external covering of organism, Proteins, disorders, STRUBBELIG, organism surface, medical condition, function, Saccaromyces cerevisiae, CC Chemokines, STRUBBELIG-RECEPTOR FAMILY 9, EVA1, Sccharomyces cerevisiae, Cytokines, Cytokine, SCYA3L, Protein, Diseases, EVA, Intercrines, Intercrine, condition, C C Chemokine, C C Chemokines, relational spatial quality, Saccharomyces oviformis, 464.2, covalent modifier, Yeast, MCP-2, LD78BETA, body surface, Dub, MCP2, Protein Gene Products, Gene Proteins, MIP1AP, disease, ligand., integumentary system, Mip1a, beta Chemokines, beta Chemokine, CC Chemokine, dermal system, T19D16.8, location, General activity, SCM, ChemokinesCharacterization, Characterized.falseEvasin RHIPU-P672 characterizationCharacterization of glycosylation sites of evasin RHIPU-P6722018-03-012017-10-23PXD008025NEWT:330879NEWT:377960NEWT:1131NEWT:35795NEWT:2042546NEWT:259447NEWT:295546NCBITaxon:2719036NEWT:1334565NEWT:625351NEWT:258594NCBITaxon:1280NEWT:112503NEWT:1129NEWT:309807NEWT:59753NEWT:89184NEWT:309800NEWT:281395NEWT:57577NEWT:1211601NEWT:876138NEWT:44271NEWT:193516NEWT:2315688NEWT:1073882NEWT:6819NEWT:112509NEWT:475174NEWT:111205NEWT:428386NEWT:1117NEWT:498257NEWT:10036NEWT:32264NEWT:12215NEWT:1590NEWT:10034NEWT:33114NEWT:1596NEWT:661410NEWT:638632NEWT:224326NEWT:376619NCBITaxon:79857NEWT:1096976NEWT:3765NEWT:3767NEWT:1589NEWT:135622NEWT:4615NEWT:67352NEWT:672127NEWT:35786NEWT:1580NEWT:399784NEWT:96731NEWT:656064NEWT:1582NEWT:3760NEWT:1584NEWT:36630NEWT:1585NEWT:383379NEWT:418106NCBITaxon:12721NEWT:10029NEWT:913645NEWT:2492962NEWT:641809NEWT:1392488NEWT:38815NEWT:317447NEWT:4688NEWT:111225NEWT:7719NEWT:5530NEWT:868565NEWT:135674NEWT:79329NEWT:30069NEWT:12637NEWT:59729NEWT:2164133NEWT:295105NEWT:29064NEWT:108061NEWT:60711NEWT:224308NEWT:273791NEWT:3347NEWT:49928NEWT:3349NEWT:160621NEWT:212790NEWT:110368NEWT:55350NEWT:1310161NEWT:77133NEWT:145481NEWT:1310165NEWT:29058NCBITaxon:79824NEWT:1912919NEWT:410658NEWT:5516NEWT:44688NEWT:44689NEWT:498211NEWT:345076NEWT:347256NEWT:5518NEWT:398007NEWT:1527468NEWT:498217NEWT:498216NEWT:11320NEWT:33554NEWT:246196NEWT:246197NEWT:145458NEWT:1310173NEWT:171719NEWT:44685NEWT:161934NEWT:156587NEWT:1148NEWT:5508NEWT:3329NEWT:419481NEWT:5507NEWT:260499NEWT:410661NEWT:1140NCBITaxon:2157NEWT:2066563NEWT:1143NEWT:1287689NEWT:1094343NEWT:1462472NEWT:1336795NEWT:644042NEWT:150808NEWT:1182590NEWT:3712NEWT:3711NEWT:3714NCBITaxon:37162NEWT:270643NEWT:2065263NEWT:177437NEWT:10891NEWT:10418NEWT:118698NEWT:1616117NEWT:118696NEWT:34865NEWT:52283NEWT:28174NEWT:284812NEWT:8175NEWT:43330NEWT:980415NEWT:1603293NEWT:44664NEWT:43346NEWT:3702NEWT:66440NEWT:1245466NEWT:3704NEWT:244366NEWT:1246791NEWT:176597NEWT:118694NEWT:2850NEWT:118691NEWT:34871NEWT:33548NEWT:52299NEWT:8165NEWT:96794NEWT:34878NEWT:3708NEWT:332648NEWT:44670NEWT:926571NEWT:536231NEWT:376219NEWT:219813NEWT:1510NEWT:460519NEWT:1515NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:1348799NEWT:33995NEWT:55784NEWT:1303443NEWT:9483NEWT:485NEWT:56636NEWT:2709072NEWT:2499525NEWT:2853422NEWT:487NEWT:1538553NEWT:1679718NEWT:480NEWT:67767NEWT:45972NEWT:46835NEWT:109757NEWT:582580NEWT:1185650NEWT:216778NEWT:294607NEWT:1502NEWT:128017NEWT:2836NEWT:376686NEWT:237610NEWT:95486EFO:0001352NEWT:119562NEWT:1883446NEWT:1233435NEWT:118223NEWT:11757NEWT:987059NEWT:109760NEWT:29031NEWT:317047NEWT:1509NEWT:491NEWT:460523NEWT:45954NEWT:235443NEWT:1579NEWT:1757312NEWT:85694NEWT:109786NEWT:108458NEWT:5936NEWT:320637NEWT:1078283NEWT:3750NEWT:983964NEWT:11706NEWT:32644NEWT:527796NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:3747NEWT:1116234NEWT:1255228NEWT:410289NEWT:191701NEWT:373153NEWT:1640278NEWT:472NEWT:940825NEWT:29459NEWT:529507NEWT:1071661NEWT:28128NEWT:470NEWT:5911NEWT:748693NCBITaxon:50557NEWT:39251NEWT:29491NEWT:101841NEWT:446NEWT:153481NEWT:2014887NEWT:33952NEWT:11723NEWT:445NEWT:153009NEWT:261756NEWT:166313NEWT:63366NEWT:63367NEWT:215402NEWT:1547NEWT:1290454NEWT:906914NEWT:81231006NEWT:28141NEWT:9031NEWT:27292NEWT:1050722NEWT:3728NEWT:108931NEWT:1293497NEWT:1055524NEWT:548NEWT:150475NEWT:267872NEWT:381666NEWT:172269NEWT:9534NEWT:2126348NEWT:5180NEWT:256737NEWT:9541NEWT:8694NEWT:33936NEWT:8692NEWT:2903NEWT:185579NEWT:872227NEWT:13076NEWT:2587410NEWT:207340NEWT:172271NEWT:1006581NEWT:8673NEWT:33940NEWT:2126337NEWT:550NEWT:33941NEWT:554NEWT:451516NEWT:552NEWT:1325291NEWT:42410NEWT:36185NEWT:1054211NEWT:1225786NEWT:771870NEWT:51338NEWT:9995NEWT:575412NEWT:28112NEWT:49012NEWT:6493NEWT:10832NEWT:6494NEWT:6491NEWT:507601NEWT:520NEWT:186441NEWT:643680NEWT:214092NCBITaxon:6157NEWT:13095NEWT:162425NEWT:104105NEWT:216257NEWT:9986NEWT:8654NEWT:8658NEWT:1268063NEWT:8657NEWT:9983NEWT:8655NEWT:5147NEWT:595495NEWT:28108NEWT:28104NEWT:407821NEWT:568703NCBITaxon:2NEWT:568708NEWT:1613NEWT:986NEWT:52641NEWT:28532NEWT:353152NEWT:298176NEWT:353153NEWT:227377NEWT:1774284NEWT:40674NEWT:1194669NEWT:51329NEWT:74426NEWT:443906NEWT:13037NEWT:519NEWT:2510939NEWT:7394NEWT:6063NEWT:1328388NEWT:105487NEWT:1548728NEWT:667127NEWT:9557NEWT:377586NEWT:7375NEWT:300641NEWT:39655NEWT:9554NEWT:38323NEWT:475932NEWT:256318NEWT:206411NCBITaxon:6191NEWT:229533NEWT:2925NEWT:214053NEWT:28550NEWT:80863NEWT:90675NEWT:52639NEWT:52638NEWT:57075NEWT:367774NEWT:1666905NEWT:8697NEWT:861109NEWT:8695NEWT:884204NEWT:510516NEWT:1045775NEWT:1123869NEWT:9544NEWT:9545NEWT:979NEWT:7370NEWT:83906NEWT:99488NEWT:1134506NEWT:255470NEWT:38783NEWT:6426NEWT:33090NEWT:9935NEWT:287889NEWT:305959NEWT:1028307NEWT:13489NEWT:29829NEWT:92867NEWT:92866NCBITaxon:3055NEWT:51750NEWT:202950NEWT:441772NEWT:1293091NEWT:1867051NEWT:48106NEWT:202956NEWT:295027NCBITaxon:11320NEWT:99490NEWT:632957NEWT:9925NCBITaxon:9606NEWT:90690NEWT:1436183NEWT:300273NEWT:4232NEWT:416348NEWT:11298NEWT:4236NEWT:7742NEWT:196627NEWT:1076878NEWT:200308NEWT:242507NEWT:200302NEWT:87043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