Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/F006057.dat-pride.xml.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/F006057.csvftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/F006057RESULT.csvftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/17.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/9.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/16.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/14.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/12.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/18.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/7.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/8.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/15.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/F006057.dat-pride.pride.mgf.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008174/F006057.datprimaryOK200002720suzhen huangNot availableKidneyExperimental Design and Statistical Rationale for Proteomics—In order to investigating the effect of miR-4331 on mitochondrial damage in response to TGEV infection, PK-15 cells were transfected with 100 nM miR-4331 mimics or negative control using Lipofectamine 3000 and infected with TGEV at an MOI of 1.0 at 24 h post-transfection (hpt). The cells were collected for quantitative proteomic analysis at 24 h post-infection (hpi). Two biological replicates were performed. To evaluate the transfection efficiency, the miR-4331 level was measured by qRT-PCR. Reverse transcription reactions and qRT-PCR were performed as described previously(17). The relative quantification of miRNAs was normalized to U6 using the two-ddCt method (18). Protein Isolation, Digestion, and Labeling with TMT—The PK-15 cells were sonicated on ice in lysis buffer (8 M urea, 1% Triton-100, 65 mM DTT and 0.1% protease inhibitor cocktail) and centrifuged at 12,000 g for 20 min at 4℃. The clarified supernatant was collected. Finally, the protein was precipitated with cold 15% TCA for 2 h at -20 ℃ and centrifuged at 4℃ for 10 min. The precipitate was redissolved in buffer (8 M urea, 100 mM TEAB, pH 8.0). 100 μg protein of each sample was digested with trypsin. The two control samples and two treatment samples were respectively labeled with TMT (126, 127,129, and 130). LC−MS/MS—The samples were fractionated by high pH reverse-phase High Performance Liquid Chromatography (HPLC). Briefly, peptides were dried by vacuum centrifugation. Then the Peptides were dissolved in 0.1% formic acid and directly loaded onto a reversed-phase pre-column (Acclaim PepMap 100, Thermo Scientific). Peptides were separated using a reversed-phase analytical column (Acclaim PepMap RSLC, Thermo Scientific) and analyzed by Q ExactiveTM hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific).PrideYong HuangQ ExactiveCOMPLETECollege of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China29217619 Zhao X, Bai X, Guan L, Li J, Song X, Ma X, Guo J, Zhang Z, Du Q, Huang Y, Tong D. microRNA-4331 promotes TGEV-induced mitochondrial damage via targeting RB1, up-regulating IL1RAP, and activating p38 MAPK pathway in vitro. Mol Cell Proteomics. 2017 10.1074/mcp.ra117.000432PTMhuangyong@nwsuaf.edu.cnMass SpectrometryShotgun proteomicsrb1transmissible gastroenteritis virusproteomicsil1rapMicror-4331mitochondrial damageMicror-4331; mitochondrial damage; proteomics; transmissible gastroenteritis virus; rb1; il1rap; p38 mapkp38 mapkhttp://www.ebi.ac.uk/pride/archive/projects/PXD008174Not availableiodoacetamide derivatized residueTMT6plex-126 reporter+balance reagent acylated residuemonohydroxylated residueAnalysis of LC-MS/MS Data—MS/MS data were analyzed by Mascot (version 2.3.0) according to a previously described protocol[17]. Briefly, raw MS data files were processed using the LC/MS software Proteome Discoverer (Thermo scientific™ software) and converted into the MASCOT generic format (mgf) files. Mascot software performed peak generation, precursor mass recalibration, extraction of TMT 6-plex reporter ions intensity and calculation of TMT 6plex reporter ions intensity ratios. For each MS/MS spectra, top 10 most intense peaks in every 100 Da window were extracted for database search. Then Mascot search was performed by searching tandem mass spectra against Uniprot Sus scrofa database (UniProt release 2015_04, 26054 entries) (http://www.uniprot.org) concatenated with reversed decoy database and protein sequences of common contaminants. Cleavage enzyme was specified as trypsin/P, maximum number of missing cleavages was set to 2. Mass tolerance for precursor ion was set to 10 ppm and mass tolerance for MS/MS ions was set to 0.02 Da. Carbamyiodomethylation on Cys and TMT6plex (N-term) and TMT6plex (K) were specified as fixed modification and oxidation on Met, acetylation on protein N-terminal were specified as variable modifications. False discovery rate (FDR) thresholds for protein, peptide and modification site were specified at 1%. Mascot software assembled the peptide/protein groups, calculated false discovery rates, filter the identifications. Protein quantification was set to use only unique peptides bearing any modification. The median ratio of TMT 6-plex reporter intensity of unique peptides was set as protein relative abundance changes. In this study, we prepared two duplicate samples. For each protein, we set the average ratio calculated from two duplicate samples as the final quantitation of protein. Student’s test was explored to calculate differential significance degree of protein relative abundance changes. T test p value less than 0.05 was considered significantly differentially. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD006864 (http://www.ebi.ac.uk/pride/archive/) [18]. Bioinformatic Analysis of Differentially Expressed Proteins—Gene Ontology (GO) annotation of proteome was derived from the UniProt-GOA database (http://www.ebi.ac.uk/GOA). Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used to annotate proteins pathways. The Wolf Psort database (https://wolfpsort.hgc.jp/) was used to predict subcellular localization. Analysis of Protein-Protein Interactions—The differentially expressed protein name identifiers (Uniprot accession) were searched against the STRING database (version 10.0) for protein-protein interactions [19]. Vector Construction—To overexpress RB1 and IL1RAP, the full-length sequences of RB1 and IL1RAP were obtained by PCR from PK-15 cells and cloned into plasmid pCI-neo (Promega, USA). The constructions were respectively named pCI-neo-RB1 and pCI-neo-IL1RAP. The primer sequences are shown in supplemental S1. Western Blot Analysis—PK-15 cells were treated with RIPA lysis buffer containing phenylmethyl sulfonylfluoride (PMSF). The proteins were separated using 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto the polyvinylidene difluoride (PVDF) membrane. The membrane was blocked with 5% BSA for 2 h at room temperature and then incubated with primary antibodies overnight at 4 ℃. The HRP-conjugated secondary antibodies were used to incubate the membrane for 1 h at room temperature. Reverse Transcription PCR and real-time PCR—Total RNA was extracted with TRIzol (Invitrogen, US). 2 μg of total RNA was treated with DNase I (Fermentas, Germany) for 30 min at 37 ℃ and reversely transcribed using the First-strand cDNA synthesis kit (Invitrogen, US). The cDNA was used as the template for real-time PCR. The real-time PCR was performed on Bio-Rad iQ5 Real-Time PCR System (Bio-Rad, USA). The reverse transcription primers and real-time PCR primers are shown in supplemental S1.ProteomicsSus Scrofa Domesticus (domestic Pig)13920817975@163.comBiologicalChina10.6019/PXD008174Transmissible gastroenteritis virus (TGEV), a member of the coronaviridae family, could cause fatal diarrhea of piglets and result in numerous economic losses. Previous studies demonstrated that TGEV infection could lead to mitochondrial damage and upregulate miR-4331 level. So miR-4331 may play an important regulatory role in the control of mitochondrial function. To explore the potential role of miR-4331 in mitochondrial damage, we adopted a strategy consisting of quantitative proteomic analysis of porcine kidney (PK-15) cells in response to miR-4331 and TGEV infection. Eventually, 69 differentially expressed proteins were gained. The target of miR-4331 was identified. The effects of miR-4331 and its target RB1 on mitochondrial Ca<sup>2+</sup> level, mitochondrial membrane potential (MMP), interleukin-1 receptor accessory protein (IL1RAP), p38 MAPK signaling pathway were investigated. The results showed that miR-4331 elevated mitochondrial Ca<sup>2+</sup> level, reduced MMP, targets Retinoblastoma 1 (RB1), upregulated IL1RAP, and induced activation of p38 MAPK pathway during TGEV infection. RB1 was identified as the direct targets of miR-4331 and downregulated IL1RAP, suppressed the activation of p38 MPAK, and attenuated TGEV-induced mitochondrial damage. In addition, IL1RAP played a positive role in activating p38 MAPK signaling and negative role in TGEV-induced mitochondrial damage. The data indicate that miR-4331 aggravates TGEV-induced mitochondrial damage by repressing expression of RB1, promoting IL1RAP, and activating p38 MAPK pathway.microRNA-4331 Promotes Transmissible Gastroenteritis Virus (TGEV)-induced Mitochondrial Damage Via Targeting RB1, Upregulating Interleukin-1 Receptor Accessory Protein (IL1RAP), and Activating p38 MAPK Pathway <i>In Vitro</i>.Zhao Xiaomin X, Bai Xiaoyuan X, Guan Lijuan L, Li Juejun J, Song Xiangjun X, Ma Xuelian X, Guo Jianxiong J, Zhang Zhichao Z, Du Qian Q, Huang Yong Y, Tong Dewen Dsodium salt, CPD photolyase activity, type 1, ammonium formate, 13C-labeled, IL1BC, cadmium salt, PhrB photolyase activity, CASP-1, bar, chronic obstructive pulmonary disease (COPD), Basodexan, magnesium formate, IDOL, zinc salt, MAGE-E1 antigen, Polypeptides, protein polypeptide chains, qRT-PCR, T22F8_160, cobalt(II) formate dihydrate, type I, responsivity, ATGPR7, Chronic irreversible airway obstruction, Urea, GRP8, GRP7, 3, NUP96, outer pigmented layer of retina, Il1bc, epithelium, HPI, Divorced, HPT, CAFL - Chronic airflow limitation, proteins, Divorces, IL-1BC, HPLC, SUPPRESSOR OF AUXIN RESISTANCE 3, AI047805, isolation and purification, pigment epithelium of retina, hydroperoxide isomerase activity, column, disease (COPD), sample, pigmented retina epithelium, Infections and Infestations, autolysin activity, Idol, nickel salt, chronic obstructive lung disease [Ambiguous], Caspase-1 subunit p10, cobalt (+2) salt, 6.3.2.-, Liquid Chromatography, LF2000, proline oxidase deficiency, CG4637, DmelCG7788, CHRONIC OBSTRUCTIVE AIRWAY DIS, sodium (4:1:1) salt, Dm1, magnesium salt, DAMAGE, Hepatocellular carcinoma-associated protein 1, dipyrimidine photolyase (photosensitive), Airflow Obstruction, GLYCINE-RICH PROTEIN 8, Chronic Obstructive Airways Disease, Chronic obstructive lung disease, Mira, formate, Infection and Infestation, cromium (+3), Inducible degrader of the LDL-receptor, beta Trypsin, ddct, F2G1.4, Thermo Scientific., OBSTRUCTIVE PULMONARY DISEASE (COPD), DDCT, Drice, MIRA, bacteriolytic toxin activity, ammonium (4:1) salt, DRICE, lead (+2) salt, TMT, hg, HH, nickel formate dihydrate, lead formate, DrIce, DrICE, aluminum salt, number, CAO - Chronic airflow obstruction, Carbamide, CHRONIC OBSTRUCTIVE PULM DIS, Chronic obstructive pulmonary disease NOS, protein-containing complex, CG7826, and RNA binding 1, deoxyribodipyrimidine photolyase activity, stratum pigmentosum retinae, Hh, Chronic obstructive pulmonary disease finding, PULMONARY DISEASE (COPD), isolation, Gm902, CG7835, CG42273, Carmol, Transcription, MONDOA, thallium (+1) salt, PCE-2, beta-Trypsin, rubidium salt, F23A5.3, AI847422, methanoic acid, (9Z, Caspase-1 subunit p20, ATGRP7, ATGRP8, bar-3, 13-hydro-lyase activity, HYRPRO1, NEC, Chronic, peptidos, 14Z)-(13S)-hydroperoxyoctadeca-9, bHLHe36, chronic obstructive airways disease NOS, Myosin regulatory light chain interacting protein, caspase 3, COAD, potassium formate, CE-2, copper (+2) salt, backward, COLD - Chronic obstructive lung disease, buffer, Cell, chronic, AW228700, polypeptide, native protein, Temperatures, carbonyldiamide, cupric formate, proteomic analysis, (COPD), chronic airway obstruction, chronic obstructive, aluminum formate, CAL - Chronic airflow limitation, AU020952, hydroperoxide dehydratase activity, lithium formate, RPE, holin, RGD1560259, hyperprolinemia caused by mutation in PRODH, Separations, GR-RBP7, GR-RBP8, lipofectamine 2000, IL-1 beta-converting enzyme, RPT, hyperprolinemia type 1, Interleukin-1 beta-converting enzyme, retinal pigment epithelium, ME-IV, p. pigmentosa retinae, 11-dienoate-forming], plan specification, linoleic acid hydroperoxide isomerase, anon-WO0182946.19, chronic obstructive lung disease (disorder), deoxyribonucleate pyrimidine dimer lyase (photosensitive), 13-hydro-lyase [(9Z)-(13S)-12, quantitative, reversed, chronic obstructive pulmonary disease, LF 2000, Reverse, T22F8.160, and rna binding 2, Chronic Airflow Obstructions, zinc formate, Infestations and Infections, CG7788, crice, lead salt, protein, lysis, pigmented epithelium, 11, Productivity, peptide, 11E, ammonium tetraformate, anon-WO0134654.19, Triton, DmelCG4637, peptido, chronic obstructive airways disease NOS (disorder), Min, Mir, MIR, Chronic Obstructive Pulmonary Disease (COPD), protein aggregate, Mrs2l, Nereid, DmelCG42273, Miranda, COLD (chronic obstructive lung disease), reference sample, dermoid cyst with malignant transformation, peptides, MRS2L, NEC in ICD9CM_2006, E927b, pigmented retina, min, mAPC, purification, mir, experimental design, copper, DNA cyclobutane dipyrimidine photolyase activity, Chronic airflow limitation, lithium salt, HCA1, ICE, GLYCINE RICH PROTEIN 7, necrosis, TEAB, glycine-rich RNA-binding protein 8, CHRONIC OBSTRUCTIVE PULMONARY DISEASE, ammonium (2:1) salt, Transfections, 9430057C20Rik, CHRONIC, PRE, obstructive lung disease, ice, iCE, drice, RT-rt PCR, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, Dmel_CG7826, pigmented retinal epithelium, Thermo Scientific, ur, Airflow Obstructions, CG12249, pulmonary disease (COPD), carbamide, S-adenosyl-L-methionine:thiol S-methyltransferase activity, transfect, retinal pigment, drIce, drICE, low temperature, CHRONIC OBSTRUCTIVE, bar3, chronic obstructive pulmonary disease and allied conditions, Chronic airway disease, bacteriocin activity, NOS, nickel (+2) salt, retinal pigment layer, Dmel_CG7835, COPD NOS, Mnb, MNB, CHRONIC OBSTRUCTIVE LUNG DIS, Chronic airway obstruction, ammonium salt, l(3)neo56, l(3)neo57, cold, retinal pigmented epithelium, P45, cobaltous formate, AW124434, Chronic Airflow Obstruction, H2NC(O)NH2, phr A photolyase activity, lysin activity, DNA-photoreactivating enzyme, precipited material, COPD - Chronic obstructive pulmonary disease, copper salt, chronic obstructive airways disease, p45, hyperprolinemia, Polypeptide, obstructive pulmonary disease (COPD), mMage-e1, 1728, COLD, chronic obstructive lung disease, DISEASE (COPD), cesium salt, circadian rhythm, photoreactivating enzyme activity, presence, Buffer, formic acid, Karbamid, method, Cold, potassium salt, Pulmonary Disease, polypeptide chain, 14C-labeled, method used in an experiment, Harnstoff, stratum pigmentosa retinae, Separated, reactivity, Cold Temperatures, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, PRECOCIOUS, Mrt, labeling, calcium formate, Chronic Obstructive, PRODH hyperprolinemia, 13-epoxyoctadeca-9, Infestation and Infection, Myosin regulatory light chain-interacting protein, LF-2000, cromium (+3) salt, sodium formate, F23A5_3, thiol methyltransferase activity, Controlled, COPD, l(3)hh, Controlling, DYRK1, COAD - Chronic obstructive airways disease, nickel formate, protein complex, not elsewhere classified, Alpha-dystrobrevin-associated MAGE Protein, strontium formate, strontium salt, PULM DIS CHRONIC OBSTRUCTIVE, Peptide, Chronic obstructive pulmonary disease finding (finding), count in organism, stratum pigmentosum (retina), MODIFIER OF SNC1, natural protein, Protein, deoxyribonucleic photolyase activity, Infection, linoleate hydroperoxide isomerase, DmelCG12249, Dyrk1, CES2A1, GLYCINE-RICH RNA-BINDING PROTEIN 7, Vacuums, teratoma with malignant transformation, chronic obstructive airway disease, 14-trienoate 12, Temperature, Separation, uree, photolyase activity, Dops, urea, CC1, Tripcellim, sample population, chromic formate, UREA, Interleukin-1 beta convertase, Trypure, RRT-PCR, 3.4.22.36, calcium salt, cold (chronic obstructive lung disease), Peptid, Chronic Obstructive Lung Disease, response, presence or absence in organismRB1, Dp38b, Interleukin 1 Receptor Accessory Protein, D-p38 MAPK, Porcine Transmissible, retinoblastoma-related 1, AI255955, DmelCG7393., FBF, CG7393, malignant, stRNA, rbf1, Il1racpb, TGE Virus, anon-sts23, micro RNA, Swine, retinoblastoma, Transmissible Gastroenteritis Virus, RBF, D-p38, autosomal dominant, dRBF, RETINOBLASTOMA 1, hereditary retinoblastoma, Gastroenteritis Virus of Swine, D-p38b, RBR, eye cancer, dp38b, IL1R3, p38 MAPK, OSRC, RB, rb, PPP1R130, Dp38, ATRBR1, RETINOBLASTOMA PROTEIN, rbf, 6430709H04Rik, p38 beta, Mpk34C, Swine Gastroenteritis Virus, p38B, pRb, p38beta, small temporal RNA, DmelCG7413, pp110, p38Kb, Rb, IL-1RAcp, Rb-1, p38, Dm p38b, INSDC_feature:ncRNA, INSDC_qualifier:miRNA, TGE Viruses, Rb1, p105-Rb, Porcine Transmissible Gastroenteritis Virus, RB - retinoblastoma, familial retinoblastoma, Dmp38b, RETINOBLASTOMA-RELATED PROTEIN 1, RETINOBLASTOMA-RELATED, C3orf13, Gastroenteritis Virus, IL-1RAcP, AV239853, CG7413, BG:DS00797.3, RbF, EG:34F3.3, microRNA, trilateral, 186F5S, Swine Gastroenteritis Viruses, somatic mutation, Rbf1, RBF1, ESTS:186F5SRB1, Ribonucleic, Materials, degree (angle), Raw, ion, GIDRP86, Il1racpb, Western, pancreatic deoxyribonuclease, rad, SDH, RBF, Long Term, 5730420M11Rik, thymonuclease activity, Polypeptides, RBR, SDS, protein polypeptide chains, KL receptor activity, Drug Tolerance, cDNA library construction, GIDRP88, SCO5, AGAMOUS-like 63, RETINOBLASTOMA PROTEIN, Western Blotting, SCO1, Software Engineering, Galpha47A, establishment and maintenance of substrate location, Analysis, rbf, Gsfsow3, CG18546, Non Polyadenylated, WMS, C10orf28, C79691, average, Galphao47A, SET, Domestic Pig, Divorced, F, DNA endonuclease activity, Analyses, G-oalpha, Genomes, M, dgo, anabolism, phosphatase 2A inhibitor I2PP2A, complementary DNA, Kitl, proteins, Divorces, W, number of, Goa, GOA, Mast cell growth factor, DNase I, G(o)alpha47A, DmelCG4063, Lccp, DmelCG4299, AI047805, set, deoxyribonuclease A, PCI-B, WESTERN BLOT, C3orf13, swine, Bs, TBL1, Tbl1, autolysin activity, pancreatic DNase activity, RG7MT1, drug tolerance, SGS, RNF100, NK/GPI, LC-MS2, Gpi, Data Set, immune system tolerance, 3.1.21.1, acetylation, pigs, Longterm Effect, somatomedin, integral to membrane, retinoblastoma, IBP1, Normalcy, antibodies, PAI-3, Spectrum Analysis, Software Tools, ACMICD, Computer Applications, PBT, Dm1, DmelCG42628, Computer Applications Software, ATRBR1, Methionin, Ribonucleic acids, Raps, NK|GPI, methionine, Hmet, dornava, PCI, PCR, pancreatic dornase, HGF/SF receptor, Acid, pbt, reticulate acropigmentation of Dohi, Software Applications, DmelCG7413, HLA-DR-associated protein II, DI-2, Source Software, I-2Dm, Stem cell factor, proportionality, Spectrometry, dyschromatosis symmetrica hereditaria 1, Deoxyribonuclease I, Dmel_CG15720, rate, MASCOT, inhibitor of granzyme A-activated DNase, hCMT1c, STAT5, study protocol, c-Met, E-2f, E-2g, Pigs, I-2PP1, stem cell factor, DNA depolymerase activity, Applications, TAF-IBETA, Self Tolerance, RB - retinoblastoma, familial retinoblastoma, Material, liquid chromatography-tandem mass spectroscopy, bacteriolytic toxin activity, deoxyribonuclease (pancreatic), TAF-Ibeta, TMT, Liquimeth, Gb, Bglap-rs1, CG2204, establishment and maintenance of cellular component location, Computer Software Applications, krk1, Boars, F10B6_15, PLANH3, AI255955, Clo, 2-amino-4-(methylsulfanyl)butanoic acid, G[[o]]alpha, Go, Tb11, False, region or site annotation, Peptidomics, Effects, Slf, SLF, Sus scrofa domestica, Acrosomal serine protease inhibitor, Sodium Lauryl, number, DNase activity, dyschromatosis symmetrica hereditaria, biosynthesis, precursor, protein-containing complex, DGalpha[[o]], dRBF, body system, DmelCG5692, Bkh, F10B6.15, CG7826, FBXW4, period, Electrophoreses, drogpad, LC-MSMS, Gene Products, CG7835, DG[[o]], CG42273, Pgi, system, RCCP2, Application, bkh, Normalities, Boar, Open Source Softwares, F15E12_6, proportion, positional, Transcription, anatomical systems, pins, Longterm, Gpi-1r, scatter factor receptor, Software Application, Gpi-1s, Phi, Gpi-1t, Open Source Software, Plasminogen activator inhibitor 3, 2pp2a, 4733401P19Rik, Long-Term, ions, PINS, CG10574, Computer Software Application, PSORT, synthesis, mKIAA0989, Con, 2PP2A, 2-amino-4-(methylthio)butanoic acid, Tools, Pig, dSET, dSet, blz, peptidos, metionina, Sulfuric acid monododecyl ester sodium salt, proto-oncogene c-Kit, RNA, CG5692, hematopoietic growth factor KL, pig, Proteins, DmelCG31211, AGL63, RNS, SMAP55, F28K20.7, DSH, DSH1, MF, backward, L-Isomer, cDNA, Amf, buffer, ribosome-associated ubiquitin-dependent protein breakdown, Cell, Tool, oligonucleotide primer, polypeptide, Sodium Lauryl Sulfate, native protein, Immune Tolerance, Temperatures, I-2PP2A, KIT ligand receptor activity, chemical analysis, Dm I-2, Long Term Effects, Escherichia coli endonuclease I, PPP1R130, MFS1, Sodium, Pins, Mass Spectrum Analyses, NK, ATCMPG1, MET, Met, ATCMPG2, XKrk1, AU020952, Go-alpha, Col4a-1, holin, Sus scrofa domesticus, tyrosine-protein kinase Met, Separations, SWDS, Ribonucleic Acid, CD117, thymonuclease, CG14732, Longterm Effects, ME-IV, plan specification, Computer Programs, Gene Proteins, Applications Softwares, localisation, binding_or_interaction_site, C-Kit, Ssm, MGF, Mgf, quotient, xkl-1, CG4346, liquid chromatography tandem mass spectrometry, AV239853, reverse transcription polymerase chain reaction, 5730455C01Rik, Sulfate, reversed, Immunological Tolerance, liquid chromatography tandem mass spectroscopy, Archive, Bru, IPP2A2, Reverse, Sodium Dodecyl, Hgfr, determination, Blotting, Somatomedin-C, Dodecyl Sulfate, cleavage, 5730555F13Rik, RT-PCR, protein, Xkl-1, CG15720, lysis, contrasted, binding site, peptide, L-Isomer Methionine, Gsfsco1, Methionine, IL1R3, ClvPrd, peptido, alkaline DNase activity, HGFR, RB, rb, Min, Gpi-1, protein aggregate, Effect, Gsfsco5, Computer Program, RNA Gene Products, DmelCG42273, multicellular organismal biosynthetic process, SOW3, Mass Spectrum Analysis, Svc, DROGPAD, single-organism biosynthetic process, rsh, LCMSMS, dermoid cyst with malignant transformation, peptides, DmelCG2204, TAF-I, MUB3_18, Nlk, pp110, Open, GORDITA, Rb, membrane region, IL-1RAcp, somatomedin-C, Computer Programs and Programming, SF, min, Lauryl Sulfate, mAPC, Domestic, MUB3.18, Galphao, IGAAD, wolf, DmelCG10574, SOLO DANCERS, IGF1, ppm, DNAase activity, Mechano growth factor, IL-1RAcP, Western Blot, CG7413, Soluble KIT ligand, necrosis, Sl, GPHYSD2, Racemethionine, Long-Term Effects, somatic mutation, Dmel_CG4346, ratio, symmetric dyschromatosis of the extremities, phapii, IGF-I, establishment and maintenance of substance location, retinoblastoma-related 1, proto-oncogene c-Met, steel factor, total RNA extract, ribose nucleic acid, PRO, T5E21.12, rbf1, LC-MS/MS, 2-Amino-4-(methylthio)butyric acid, ribonucleic acids, Dmel_CG7826, StF-IT-1, AI836084, Tr-kit, extra or missing physical or functional parts, membranous organ component, 9130215G10Rik, anon-WO03070958.3, CYS, RETINOBLASTOMA 1, Source Softwares, hereditary retinoblastoma, sKITLG, PAI3, Programs, Spectroscopy, Program, single organism localization, S-adenosyl-L-methionine:thiol S-methyltransferase activity, pigs <Sus scrofa>, mOC-X, Ionen, mechano growth factor, Ribonukleinsaeure, Computer Applications Softwares, Immunologic Tolerance, kl1-A, Genetic Materials, bacteriocin activity, pentosenucleic acids, median, Softwares, KIT, Igf-1, and GLY protein 2, 6430709H04Rik, region, Genetic Material, Dmel_CG7835, T5E21_12, parent ion, HGF receptor, Mnb, MNB, pRb, tyrosine-protein kinase Kit, SHEP7, ORG, Org, and GLY protein 1, membrane of organ, positional polypeptide feature, CG4063, mast cell growth factor, ribosome-associated degradation, endodeoxyribonuclease I, familial reticulate acropigmentation of Dohi, Gpi1-r, common, Gpi1-s, ribosome-associated ubiquitin-dependent protein degradation, MASS, CG4299, kit, AW124434, Gpi1-t, REM3, organ system, Irium, Kitlg, goalpha, p105-Rb, metastatic, Health, KL-1, lysin activity, precursor ion, Tolerance, KITLG, Cistron, Polypeptide, EG:34F3.3, i2pp2a, RAD1, Proteomes, time, Rbf1, RBF1, Gpi1s, Galpha[[o]], AW549739, AI461847, FBF, L Isomer, SCF receptor activity, FBN, dornavac, rapsyn, Gene, FPH2, Computer, Spectrum Analyses, dgoalpha, Serpin A5, autosomal dominant, PHAPII, LC-MS-MS, Buffer, method, eye cancer, HGF, polypeptide chain, template-activating factor I, scfr, ECTOL1, integral component of membrane, method used in an experiment, Mass, establishment and maintenance of position, OSRC, L-Methionine, F15E12.6, Del(8)44H, wild boar, Separated, SCFR, Pedameth, Mass Spectroscopy, PROCI, Pro-Mega, study, Fdc, Galpha[[047A]], Genetic, alkaline deoxyribonuclease activity, formation, iones, EBI, Ebi, ipp2a2, Acetylations, blocked, deoxyribonucleic phosphatase activity, Domestic Pigs, AUTS9, G[[o]], OCTD, Non-Polyadenylated RNA, 10^[-6], taf-ibeta, PCR., has or lacks parts of type, region of membrane, site, ribosome-associated ubiquitin-dependent protein catabolism, Long-Term Effect, AI838057, G[[oalpha]], Wild Boars, thiol methyltransferase activity, Applications Software, Open Source, DGalpha0, membrane, data, DYRK1, Galpha[[0]], Encyclopedias, CG42628, Computer Software, DL-Methionine, protein complex, malignant, synthesize, Wild Boar, igaad, total expressed protein, Protein C inhibitor, acropigmentation of Dohi, Sus scrofus, Goalpha, Cistrons, Peptide, mereological quality, LC/MS/MS, Software Tool, natural protein, yeast nucleic acid, Protein, dornase activity, I2PP2A, whole membrane, establishment and maintenance of localization, Dyrk1, connected anatomical system, Software, DNA nuclease activity, WMS2, Data Base, teratoma with malignant transformation, Mass Spectrum, ribonucleic acid, Wild, Separation, c-KIT, transmembrane, G-alpha-47A, Normality, INSDC_feature:misc_binding, Non Polyadenylated RNA, CC1, Engineering, Rb-1, Non-Polyadenylated, arc degree, single-organism localization, CGI-97, alpha(o), l(2)k16213, Protein Gene Products, Rb1, dSET/TAF-Ibeta, F28K20_7, 2610030F17Rik, c-kit, Ion, c-Kit ligand, SSKS, cardinality, RETINOBLASTOMA-RELATED PROTEIN 1, RETINOBLASTOMA-RELATED, alpha-amino-gamma-methylmercaptobutyric acid, Peptid, G-oalpha47a, RAD, Rad, assay, Par4, SCF, RbF, variable, AA407739, trilateral, SF receptorRB1, extracellular signal-regulated kinase activity, GrpL, DmErk, pp44mapk, GRPL, Kinship, Growth factor receptor-binding protein, CRKII, The runs, NURF38, Sporadic Retinoblastomas, Il1racpb, bar, RBF, IDOL, p38-alpha, p38MAPK, MAGE-E1 antigen, p38a, Prp4 protein kinase activity, Hog, p38ALPHA, RBR, MAP kinase 1 activity., benign mucosal pemphigoid, LeMPK3, "retinoblastoma" EXACT [CSP2005:2018-3452], calcium(2+) ion, ATP-protein transphosphorylase activity, p44mpk, hnRNP A2/B1, responsivity, ATP:protein phosphotransferase (non-specific) activity, Dp38, Life Cycle, RETINOBLASTOMA PROTEIN, rbf, SAPK2, Mpk34C, SEM, Sem, p38B, p38A, MAP-k, mapk14a, Kinship Network, benign mucous membrane pemphigoid, pp42, diarrheal disease, hrp40, Torovirinae, stress-activated MAPK signaling pathway, hnRNP36, Dsor2, proteins, Familial, elevated, Dm p38b, Hrb87Fa, sem, cicatricial pemphigoid with ocular involvement, Hrb85CD, decreased, hrp36, Grf40, CP, Role Concepts, C3orf13, dMKK3, Infections and Infestations, Idol, GRBLG, ESTS:186F5S, DmMPK2, reniculate kidney, p82 kinase activity, ERK-A, dpERK, dpErk, 6.3.2.-, csbp, ERK-2, betaIIPKC, Coronavirinae, CG4634, retinoblastoma, DmMAPK, PMK-2, PMK-1, dp-ERK, signal transduction by trans-phosphorylation, CG4637, PMK-3, Hrb87f, CSBP, Wee-kinase activity, p38 MAPK signalling, diarrheal disorder, Role Concept, Double ring-finger protein, Playthings and Play, DAMAGE, Hepatocellular carcinoma-associated protein 1, Role, pMAPK, pMapK, ATRBR1, Bafinivirus, D-MPK2, GRAP-2, Diarrhoea, activation, Retinal, DmelCG5475, Mek3, MEK3, DMEK3, DmERKA, DmelCG7413, Glioblastoma, rl/MAPK, Mira, "Retinoblastoma (morphologic abnormality)" EXACT [SNOMEDCT_2005_07_31:19906005], non-specific serine/threonine protein kinase activity, Infection and Infestation, Inducible degrader of the LDL-receptor, l(2)41Ac, c-Crk, Hereditary Retinoblastoma, NURF-38, CSBP2, MAPK13 cascade, CSBP1, CT34260, dpERk, calcium(II) cation, signaling pathway, RB - retinoblastoma, familial retinoblastoma, serine-specific protein kinase activity, MIRA, HIPK2, SAPK, cytidine 3', Gastroenteritis Virus, Csbp2, Glioma, Csbp1, Wee 1-like kinase activity, hg, HH, D-p38 MAPK, Grf40 adapter protein, AI255955, glycogen synthase A kinase activity, glycogen synthase kinase 3 activity, number, 12559, Transmissible Gastroenteritis Virus, GRB-2-like protein, Exip, dRBF, phosphorylase b kinase kinase activity, EK2-1, Hh, PRKM15, benign mucous Membrane pemphigoid, loose stools, dMPK2, p38 MAPK, PRKM14, Gene Products, sapk2a, mucous membrane pemphigoid, ribosomal protein S6 kinase II activity, dNURF, EXIP, Swine Gastroenteritis Virus, Toy, ERK, Erk, Eye Cancers, PRKM1, p38beta, PRKM2, protein-serine kinase activity, Hrp36, Retinoblastoma Eye, JTV1, MONDOA, p38Ka, Playthings, Retinoblastoma Eye Cancers, Retinal Neuroblastomas, p38Kb, STK32, calcium(2+), AI847422, AI848995, BC023857, MAPK11 cascade, GRB2L, erk, CG12749, Hereditary Retinoblastomas, Puppets, protein-cysteine kinase activity, stress-activated MAPKKK signalling pathway, bar-3, Hpr kinase activity, rll, HRP36, benign mucous membrane pemphigoid with ocular involvement, bHLHe36, STK26, p38-2, "Retinoblastomas (morphologic abnormality)" EXACT [SNOMEDCT_2005_07_31:189934006], high elevation, myelin basic protein kinase activity, Puppet, Cancer, Dp38b, Dp38a, RING finger protein 19A, SAPK2A, Family Research, Myosin regulatory light chain interacting protein, Proteins, stress-activated kinase activity, MAP-2 kinase activity, Protein GADS, function, cictricial pemphigoid with ocular involvement, protein serine kinase activity, Cell, Gastroenteritis Virus of Swine, protein phosphokinase activity, Concept, Family Members, AW228700, DmMKK3, PDIP38, MAPK12 cascade, PKA, PKC, p38MAPKK, stress-activated protein kinase activity, mapk2, proteomic analysis, mapk1, PPP1R130, signalling cascade, p38 beta, protein kinase p58 activity, Nurf38, serine/threonine protein kinase activity, Watery stool, CG12559, underdeveloped, RGD1560259, ocular pemphigoid, P42MAPK, vegetating cicatricial pemphigoid, dpERK1, Glioblastomas, Crk1, Cancers, Crk3, CSPB1, dsk1, 5'-cyclic monophosphate-responsive protein kinase activity, atypical PKC activity, Gene Proteins, 38 kDa DNA polymerase delta interaction protein, D-MKK3, anon-WO0182946.19, dpMAPK, CRK1, White bream viruses, Families, AV239853, quantitative, matrix metalloproteinase, Relatives, protein serine-threonine kinase activity, SAP kinase activity, glycogen synthase kinase activity, Networks, Nurf 38, protein kinase A activity, sapk2, Mpk2, Mpk3, TGE Virus, Infestations and Infections, Crko, p42mapk, Swine, D-p38, Crk-II, p38 alpha, NURF, SR2-1, DmelCG4634, anon-WO0134654.19, IL1R3, DmelCG4637, p38/NURF-38, Roles, kinase-related transforming protein, RB, rb, Concepts, mucosal pemphigoid, mitogen-activated protein kinase activity, Q14, Mir, MIR, Q16, RK, mpk2, present in fewer numbers in organism, mpk1, Family Life Cycle, Miranda, signal transduction by protein phosphorylation, Adapter protein GRID, Erk/Map kinase, DMKK3, reference sample, CALCIUM ION, pp110, Diarrheas, MEK3/MKK3, Rb, IL-1RAcp, hypoplasia, DERK-A, mir, E(sina)7, Rl, Raf kinase activity, MP kinase activity, serine kinase activity, 38, DERK, Dmp38a, AHA1, atypical protein kinase C activity, HCA1, Hematopoietic cell-associated adapter protein GrpL, Dmp38b, Retinal Glioblastoma, MBP kinase II activity, DmelCG12749, IL-1RAcP, P11, mitogen-activated S6 kinase activity, protein-aspartyl kinase activity, CG7413, dERK, Family Life Cycles, CG5475, somatic mutation, 9430057C20Rik, retinoblastoma-related 1, MAP kinase 2 activity, Mapk, rbf1, signal transduction by conformational transition, "Retinoblastoma NOS (morphologic abnormality)" EXACT [SNOMEDCT_2005_07_31:189935007], Erk1, ERK1, ERK2, mapk1a, Erk2, A230093K24Rik, The trots, Hrb2, RETINOBLASTOMA 1, hereditary retinoblastoma, results, D-P38a, doubly charged positive ion, GRBX, GrbX, CG12249, D-p38b, M phase-specific cdc2 kinase activity, D-p38a, signaling cascade, p41mapk, Syn, MapK, mapk1b, MAPK, diarrhea of presumed infectious origin, bar3, Retinal Glioblastomas, Plaything, MMP, P38, Gliomas, p38 cascade, F20P5.11, Filiation, C14orf3, 6430709H04Rik, serine protein kinase activity, erk2, mapk, SAPK cascade, CG12244, HRB87F/hrp36, pRb, White bream virus, phosphorylase B kinase kinase activity, ERKa, F20P5_11, Familial Retinoblastoma, Retinoblastoma Eye Cancer, SH3-SH2-SH3 adapter Mona, Retinal Gliomas, l(3)neo56, l(3)neo57, GADS, BcDNA:RE08694, protein glutamyl kinase activity, Neuroblastomas, JTV-1, Bafiniviruses, l(2R)EMS45-39, HRB87F, Mona, Toys, DmelCG12559, p38alpha, p38, Prkm15, Ca(2+), Prkm14, benign mucous membrance pemphigoid, Proto-oncogene c-Crk, DpErk, DpERK, Life Cycles, ocular pemphigus, ErkA, Kidneys, p105-Rb, calcium, ERKA, hydroxyalkyl-protein kinase activity, WEE1Hu, Hereditary, p41, epsilon PKC, p40, DmelCG12304, EG:34F3.3, 186F5S, Swine Gastroenteritis Viruses, mMage-e1, Rbf1, RBF1, pERK, membrane potential of mitochondrial membrane, Interleukin 1 Receptor Accessory Protein, stress-activated MAPK signalling pathway, Porcine Transmissible, ribosomal S6 protein kinase activity, Sporadic, calcium/phospholipid-dependent protein kinase activity, Plays, l(1)G0252, Family Member, GroupII, FBF, Retinal Neuroblastoma, frequent stools, CG7393, Gene, Network, stress-activated MAPKKK signaling pathway, presence, autosomal dominant, CrkIII, p38Hog, dp38a, eye cancer, dp38b, Ca2+, Gads, reduced, subnumerary, MAPK14 cascade, Eye Cancer, OSRC, "neuroblastoma of Retina" EXACT [NCI2004_11_17:C6956], N38, serine(threonine) protein kinase activity, p38 MAPK signaling, tiny, p42-MAPK, Retinal Glioma, attenuated, T-antigen kinase activity, galactosyltransferase-associated kinase activity, reactivity, Mkk3, MKK3, Sporadic Retinoblastoma, "RB - Retinoblastoma" EXACT [SNOMEDCT_2005_07_31:134191003], Research, DmelCG12244, Mrt, xp42, Grf-40, decreased number, p42 mitogen-activated protein kinase activity, Crk-III, CG18732, ATP:protein phosphotransferase (MAPKK-activated) activity, TGE Viruses, Infestation and Infection, protein kinase (phosphorylating) activity, signalling pathway, Myosin regulatory light chain-interacting protein, Mucosynechial pemphigoid, GRID, Raf-1, Play, signal transduction by cis-phosphorylation, DmERK-A, Kinship Networks, Family, casein kinase (phosphorylating) activity, Controlled, small, l(3)hh, Controlling, data, ert1, DMPK2, malignant, Alpha-dystrobrevin-associated MAGE Protein, prkm2, anon-sts23, prkm1, CT39192, P11/Hrb87F, stress-activated MAPKKK cascade, diarrhoea of presumed infectious origin, Crk-I, DMKK3/lic, DmelCG7393, p38delta, count in organism, twitchin kinase activity, Diarrhea, group 2, diarrhea, Protein, AP50 kinase activity, "RB" EXACT [NCI2004_11_17:C7541], Infection, MBP kinase I activity, "Retinoblastoma (disorder)" EXACT [SNOMEDCT_2005_07_31:370967009], DmelCG12249, Retinoblastomas, Retinoblastoma, l(2)k16102, Xp42, threonine-specific protein kinase activity, Familial Retinoblastomas, Neuroblastoma, diarrhoea, mitogen activated kinase activity, Su(Raf)2B, Mxi2, POLD4, Rb-1, cicatricial pemphigoid, Sapk2A, EY2-2, MAPK2, dp38, Dorfin, Protein Gene Products, Rb1, Porcine Transmissible Gastroenteritis Virus, D-ERK, mxi2, ERT1, RETINOBLASTOMA-RELATED PROTEIN 1, RETINOBLASTOMA-RELATED, BG:DS00797.3, response, RbF, trilateral, MAP kinase 1 activity, A-kinase activity, presence or absence in organismRB1, extracellular signal-regulated kinase activity, GrpL, pp44mapk, GRPL, Kinship, Growth factor receptor-binding protein, CRKII, The runs, FON1, NURF38, Il1racpb, bar, RBF, FLORAL ORGAN NUMBER 1, IDOL, p38-alpha, p38MAPK, p38a, Hog, p38ALPHA, RBR, benign mucosal pemphigoid, LeMPK3, p44mpk, hnRNP A2/B1, responsivity, Dp38, Life Cycle, RETINOBLASTOMA PROTEIN, rbf, SAPK2, Mpk34C, p38B, p38A, mapk14a, Kinship Network, benign mucous membrane pemphigoid, pp42, diarrheal disease, hrp40, Bdr, Torovirinae, stress-activated MAPK signaling pathway, hnRNP36, proteins, elevated, Dm p38b, Hrb87Fa, cicatricial pemphigoid with ocular involvement, SNAP-25, Hrb85CD, decreased, hrp36, Grf40, CP, Role Concepts, C3orf13, dMKK3, Infections and Infestations, Idol, GRBLG, ESTS:186F5S, DmMPK2, reniculate kidney, 6.3.2.-, csbp, ERK-2, Coronavirinae, CG4634, retinoblastoma, PMK-2, PMK-1, CG4637, PMK-3, Hrb87f, CSBP, p38 MAPK signalling, diarrheal disorder, Role Concept, ATP synthase D chain, Double ring-finger protein, Playthings and Play, Role, ATRBR1, GENA70, Bafinivirus, D-MPK2, GRAP-2, Diarrhoea, activation, DmelCG5475, Mek3, MEK3, DMEK3, DmelCG7413, Mira, Infection and Infestation, Inducible degrader of the LDL-receptor, c-Crk, NURF-38, CSBP2, MAPK13 cascade, CSBP1, RB - retinoblastoma, familial retinoblastoma, MIRA, Gastroenteritis Virus, Csbp2, Csbp1, hg, FLORAL DEFECTIVE 10, HH, D-p38 MAPK, Grf40 adapter protein, AI255955, number, SUPERMAN, Transmissible Gastroenteritis Virus, mitochondrial, GRB-2-like protein, Exip, dRBF, Hh, PRKM15, benign mucous Membrane pemphigoid, loose stools, dMPK2, p38 MAPK, PRKM14, Gene Products, sapk2a, mucous membrane pemphigoid, dNURF, EXIP, Swine Gastroenteritis Virus, Toy, ERK, PRKM1, p38beta, PRKM2, Hrp36, JTV1, MONDOA, p38Ka, Playthings, p38Kb, AI848995, BC023857, MAPK11 cascade, GRB2L, CG12749, Puppets, stress-activated MAPKKK signalling pathway, bar-3, HRP36, benign mucous membrane pemphigoid with ocular involvement, bHLHe36, STK26, p38-2, high elevation, myelin basic protein kinase activity, Puppet, Dp38b, Dp38a, RING finger protein 19A, SAPK2A, Family Research, Myosin regulatory light chain interacting protein, Proteins, MAP-2 kinase activity, Protein GADS, function, Synaptosomal-associated 25 kDa protein, cictricial pemphigoid with ocular involvement, Cell, Gastroenteritis Virus of Swine, Concept, Family Members, AW228700, DmMKK3, PDIP38, MAPK12 cascade, p38MAPKK, proteomic analysis, PPP1R130, p38 beta, Nurf38, Watery stool, underdeveloped, ocular pemphigoid, P42MAPK, vegetating cicatricial pemphigoid, Crk1, Crk3, CSPB1, Gene Proteins, 38 kDa DNA polymerase delta interaction protein, D-MKK3, anon-WO0182946.19, CRK1, White bream viruses, Families, AV239853, quantitative, matrix metalloproteinase, Relatives, Networks, Nurf 38, sapk2, Mpk2, Mpk3, TGE Virus, Infestations and Infections, Crko, p42mapk, Swine, D-p38, Crk-II, p38 alpha, NURF, DmelCG4634, anon-WO0134654.19, IL1R3, DmelCG4637, p38/NURF-38, Roles, SUP, RB, rb, Concepts, mucosal pemphigoid, mitogen-activated protein kinase activity, Q14, Mir, MIR, Q16, RK, mpk2, present in fewer numbers in organism, Family Life Cycle, Miranda, Adapter protein GRID, DMKK3, reference sample, pp110, Diarrheas, MEK3/MKK3, Rb, IL-1RAcp, hypoplasia, mir, Super protein, sp, MP kinase activity, 38, Dmp38a, AHA1, Dmp38b, Hematopoietic cell-associated adapter protein GrpL, MBP kinase II activity, DmelCG12749, IL-1RAcP, P11, CG7413, Family Life Cycles, CG5475, somatic mutation, 9430057C20Rik, retinoblastoma-related 1, MAP kinase 2 activity, rbf1, ERK1, ERK2, Erk2, A230093K24Rik, The trots, Hrb2, RETINOBLASTOMA 1, hereditary retinoblastoma, results, D-P38a, GRBX, GrbX, CG12249, D-p38b, D-p38a, p41mapk, Syn, MAPK, diarrhea of presumed infectious origin, bar3, Plaything, MMP, P38, p38 cascade, F20P5.11, Filiation, C14orf3, 6430709H04Rik, SAPK cascade, CG12244, HRB87F/hrp36, pRb, White bream virus, Mitochondrial, F20P5_11, SH3-SH2-SH3 adapter Mona, l(3)neo56, l(3)neo57, GADS, JTV-1, Bafiniviruses, HRB87F, Mona, Toys, p38alpha, p38, Prkm15, Prkm14, benign mucous membrance pemphigoid, Proto-oncogene c-Crk, Life Cycles, ocular pemphigus, Kidneys, p105-Rb, p41, p40, DmelCG12304, EG:34F3.3, 186F5S, Swine Gastroenteritis Viruses, Rbf1, RBF1, membrane potential of mitochondrial membrane, Interleukin 1 Receptor Accessory Protein, stress-activated MAPK signalling pathway, Porcine Transmissible, Plays, l(1)G0252, Family Member, FBF, frequent stools, CG7393, Gene, Network, stress-activated MAPKKK signaling pathway, presence, autosomal dominant, CrkIII, p38Hog, dp38a, eye cancer, dp38b, Gads, reduced, subnumerary, MAPK14 cascade, OSRC, N38, p38 MAPK signaling, tiny, p42-MAPK, attenuated, reactivity, Mkk3, MKK3, Research, DmelCG12244, Mrt, Grf-40, decreased number, p42 mitogen-activated protein kinase activity, Crk-III, ATP:protein phosphotransferase (MAPKK-activated) activity, TGE Viruses, Infestation and Infection, Mif1, Myosin regulatory light chain-interacting protein, Mucosynechial pemphigoid, GRID, Play, Kinship Networks, Family, Controlled, small, l(3)hh, Controlling, data, DmelCG7393., DMPK2, malignant, anon-sts23, P11/Hrb87F, stress-activated MAPKKK cascade, diarrhoea of presumed infectious origin, Crk-I, DMKK3/lic, DmelCG7393, p38delta, count in organism, Diarrhea, group 2, diarrhea, Protein, Infection, MBP kinase I activity, DmelCG12249, l(2)k16102, diarrhoea, mitogen activated kinase activity, Mxi2, POLD4, Rb-1, cicatricial pemphigoid, Sapk2A, FLO10, MAPK2, HERP, dp38, Dorfin, Protein Gene Products, Rb1, Porcine Transmissible Gastroenteritis Virus, mxi2, ERT1, RETINOBLASTOMA-RELATED PROTEIN 1, RETINOBLASTOMA-RELATED, BG:DS00797.3, response, RbF, trilateral, MAP kinase 1 activity, presence or absence in organismRB1, extracellular signal-regulated kinase activity, GrpL, DmErk, pp44mapk, GRPL, Growth factor receptor-binding protein, CRKII, NURF38, Il1racpb, micro RNA, RBF, p38-alpha, p38MAPK, MAGE-E1 antigen, p38a, Prp4 protein kinase activity, Hog, p38ALPHA, RBR, MAP kinase 1 activity., LeMPK3, ATP-protein transphosphorylase activity, p44mpk, hnRNP A2/B1, ATP:protein phosphotransferase (non-specific) activity, Dp38, RETINOBLASTOMA PROTEIN, rbf, SAPK2, Mpk34C, SEM, Sem, p38B, p38A, MAP-k, mapk14a, pp42, hrp40, hnRNP36, Dsor2, Dm p38b, Hrb87Fa, sem, Hrb85CD, hrp36, Grf40, C3orf13, dMKK3, GRBLG, ESTS:186F5S, DmMPK2, p82 kinase activity, ERK-A, dpERK, dpErk, 6.3.2.-, csbp, ERK-2, betaIIPKC, CG4634, retinoblastoma, DmMAPK, PMK-2, PMK-1, dp-ERK, PMK-3, Hrb87f, CSBP, Wee-kinase activity, Double ring-finger protein, DAMAGE, Hepatocellular carcinoma-associated protein 1, pMAPK, pMapK, ATRBR1, D-MPK2, GRAP-2, DmelCG5475, Mek3, MEK3, DMEK3, DmERKA, DmelCG7413, rl/MAPK, non-specific serine/threonine protein kinase activity, l(2)41Ac, c-Crk, NURF-38, CSBP2, CSBP1, CT34260, dpERk, RB - retinoblastoma, familial retinoblastoma, serine-specific protein kinase activity, HIPK2, SAPK, cytidine 3', Csbp2, Csbp1, microRNA, Wee 1-like kinase activity, D-p38 MAPK, Grf40 adapter protein, AI255955, glycogen synthase A kinase activity, stRNA, glycogen synthase kinase 3 activity, 12559, GRB-2-like protein, Exip, dRBF, phosphorylase b kinase kinase activity, EK2-1, PRKM15, dMPK2, p38 MAPK, PRKM14, sapk2a, ribosomal protein S6 kinase II activity, dNURF, EXIP, ERK, Erk, PRKM1, p38beta, PRKM2, protein-serine kinase activity, Hrp36, JTV1, p38Ka, p38Kb, STK32, AI847422, AI848995, BC023857, GRB2L, erk, CG12749, protein-cysteine kinase activity, Hpr kinase activity, rll, HRP36, STK26, p38-2, myelin basic protein kinase activity, Dp38b, Dp38a, RING finger protein 19A, SAPK2A, stress-activated kinase activity, MAP-2 kinase activity, Protein GADS, protein serine kinase activity, protein phosphokinase activity, DmMKK3, PDIP38, PKA, PKC, p38MAPKK, stress-activated protein kinase activity, mapk2, mapk1, PPP1R130, p38 beta, protein kinase p58 activity, Nurf38, serine/threonine protein kinase activity, CG12559, RGD1560259, P42MAPK, dpERK1, Crk1, Crk3, CSPB1, dsk1, 5'-cyclic monophosphate-responsive protein kinase activity, atypical PKC activity, 38 kDa DNA polymerase delta interaction protein, D-MKK3, dpMAPK, CRK1, AV239853, protein serine-threonine kinase activity, SAP kinase activity, glycogen synthase kinase activity, Nurf 38, protein kinase A activity, sapk2, Mpk2, Mpk3, Crko, p42mapk, D-p38, Crk-II, p38 alpha, NURF, SR2-1, DmelCG4634, IL1R3, p38/NURF-38, kinase-related transforming protein, RB, rb, mitogen-activated protein kinase activity, Q14, Q16, RK, mpk2, mpk1, Adapter protein GRID, Erk/Map kinase, DMKK3, pp110, MEK3/MKK3, Rb, IL-1RAcp, DERK-A, E(sina)7, Rl, INSDC_feature:ncRNA, Raf kinase activity, MP kinase activity, serine kinase activity, 38, DERK, Dmp38a, AHA1, atypical protein kinase C activity, HCA1, Hematopoietic cell-associated adapter protein GrpL, Dmp38b, MBP kinase II activity, DmelCG12749, IL-1RAcP, P11, mitogen-activated S6 kinase activity, protein-aspartyl kinase activity, CG7413, dERK, CG5475, somatic mutation, retinoblastoma-related 1, MAP kinase 2 activity, Mapk, rbf1, Erk1, ERK1, ERK2, mapk1a, Erk2, A230093K24Rik, Hrb2, RETINOBLASTOMA 1, hereditary retinoblastoma, D-P38a, GRBX, GrbX, D-p38b, M phase-specific cdc2 kinase activity, D-p38a, p41mapk, Syn, MapK, mapk1b, MAPK, P38, C14orf3, 6430709H04Rik, serine protein kinase activity, erk2, mapk, CG12244, HRB87F/hrp36, pRb, phosphorylase B kinase kinase activity, ERKa, SH3-SH2-SH3 adapter Mona, GADS, BcDNA:RE08694, protein glutamyl kinase activity, JTV-1, l(2R)EMS45-39, HRB87F, Mona, DmelCG12559, p38alpha, p38, Prkm15, Prkm14, INSDC_qualifier:miRNA, Proto-oncogene c-Crk, DpErk, DpERK, ErkA, p105-Rb, ERKA, hydroxyalkyl-protein kinase activity, WEE1Hu, p41, epsilon PKC, p40, DmelCG12304, EG:34F3.3, 186F5S, mMage-e1, Rbf1, RBF1, pERK, ribosomal S6 protein kinase activity, calcium/phospholipid-dependent protein kinase activity, l(1)G0252, GroupII, FBF, CG7393, autosomal dominant, CrkIII, p38Hog, dp38a, eye cancer, dp38b, Gads, OSRC, N38, serine(threonine) protein kinase activity, p42-MAPK, T-antigen kinase activity, galactosyltransferase-associated kinase activity, Mkk3, MKK3, small temporal RNA, DmelCG12244, xp42, Grf-40, p42 mitogen-activated protein kinase activity, Crk-III, CG18732, ATP:protein phosphotransferase (MAPKK-activated) activity, protein kinase (phosphorylating) activity, GRID, Raf-1, DmERK-A, casein kinase (phosphorylating) activity, ert1, DMPK2, malignant, Alpha-dystrobrevin-associated MAGE Protein, prkm2, anon-sts23, prkm1, CT39192, P11/Hrb87F, Crk-I, DMKK3/lic, DmelCG7393, p38delta, twitchin kinase activity, group 2, AP50 kinase activity, MBP kinase I activity, l(2)k16102, Xp42, threonine-specific protein kinase activity, mitogen activated kinase activity, Su(Raf)2B, Mxi2, POLD4, Rb-1, Sapk2A, EY2-2, MAPK2, dp38, Dorfin, Rb1, D-ERK, mxi2, ERT1, RETINOBLASTOMA-RELATED PROTEIN 1, RETINOBLASTOMA-RELATED, BG:DS00797.3, RbF, trilateral, MAP kinase 1 activity, A-kinase activity272truemicroRNA-4331 promotes TGEV-induced mitochondrial damage via targeting RB1, up-regulating IL1RAP, and activating p38 MAPK pathway in vitroTransmissible gastroenteritis virus (TGEV), a member of the coronaviridae family, could cause fatal diarrhea of piglets and result in numerous economic losses. Previous studies demonstrated that TGEV infection could lead to mitochondrial damage and up-regulate miR-4331 level. So miR-4331 may play an important regulatory role in the control of mitochondrial function. To explore the potential role of miR-4331 in mitochondrial damage, we adopted a strategy consisting of quantitative proteomic analysis of porcine kidney (PK-15) cells in response to miR-4331 and TGEV infection. Eventually, 69 differentially expressed proteins were gained. The target of miR-4331 was identified. The effects of miR-4331 and its target RB1 on mitochondrial Ca2+ level, mitochondrial membrane potential (MMP), interleukin-1 receptor accessory protein (IL1RAP), p38 MAPK signaling pathway were investigated. The results showed that miR-4331 elevated mitochondrial Ca2+ level, reduced MMP, targets Retinoblastoma 1 (RB1), up-regulated IL1RAP, and induced activation of p38 MAPK pathway during TGEV infection. RB1 was identified as the direct targets of miR-4331 and down-regulated IL1RAP, suppressed the activation of p38 MPAK, and attenuated TGEV-induced mitochondrial damage. In addition, IL1RAP played a positive role in activating p38 MAPK signaling and negative role in TGEV-induced mitochondrial damage. The data indicate that miR-4331 aggravates TGEV-induced mitochondrial damage by repressing expression of RB1, promoting IL1RAP, and activating p38 MAPK pathway.2017-12-152017-11-09PXD008174280655164609918049829535851114528421880905811363336351524260119832758385NCBITaxon:128068888561896111430118726355557114526236493461327467073853883641135461582158236889547627211232944222911809923233654583334607925193659143205971293865581914932637371250232NCBITaxon:1272111957748186614885843451358672217554374081691804542668344530586126683514982250581496367458554528113208147956410039237514636942049433208964208963445974137979128384400667935293671521659940483216597216595455013152826930421659156271616968332183217292042779447623764543172030949801975255569781439843948840479NCBITaxon:2157525338551531051067439899037122857759515205968762311210884555588845587077918178081736309431794571698936160488409859NCBITaxon:371621047826794015059071483643204210613204913816936303555432046632633311994565453511025411103217459216129410026420369456941016763410210761277351131658239254727933428146135843017463345774449167547604893212873567568403019172651813019411192943936711041131023932025559863258278839659104759347495165299835525663175854310243260866310245980875741755518320196447145268045796190832331156114155421932555217634123598755529NCBITaxon:475185025476113615741465477180066556484337677299767982510216998238976413735115571108972444474153NCBITaxon:5055789969844100036465349334147548019467512991143597412764731370863265478765698353221599838192011103481985247946409860995861571871766859919157295137221171101107687834974198601488406334249235985988655974649987039202111072943311711397215987419085037117082841823502414511391902197822474636046147123743759392532566489050794526591972211915853101774208588596NCBITaxon:61575052102830798951105198997237505957613172000263419610264940772275689964975157244NCBITaxon:22023717NCBITaxon:1703612201999857050617476792745062724915961533355418400524643213686150596866629417347515363291018194301086731018557303963558633149286287132161865368595105135260710185687294296110091416333NCBITaxon:619184023210007348824187878645159812863577553470951451225786260704NCBITaxon:1335626260705260707658457405416233150561007133705654195NCBITaxon:3055504457913998781681928752829126806315327341478281055524NCBITaxon:9606469008150251930283672859300852NCBITaxon:569382688306401794699151011849915154824283811160419370475927237082816772850351581990119519532673863702492405360886224888175626158355837153337605726711935019980861193502167879371137127240737145712562745757270903113335503728142433747924910078711082NCBITaxon:961559463318586260799141262200298492670NCBITaxon:1263719281249212528303856838569591169266100658123756127504542666255151978837503600941293497905435247214012576499085151514389923745374744544641524323592887051580595536157932716029385114978633892894535936479437NCBITaxon:6854412754225117159010901341673871589552101296610306129671585158451584937659393432715910022637673388942345128768946704146479293657616428034216413315992803828035369091089176894621812141080348179392287889295027113117493468192273218903821076012471904007271143114020641198108713554774365814245072295337969820423212422312916321803347087145372091645733157546992877702020603281143193382935508332912922114872526499972830357275507176299707678926837041521405329412816078395639242273775962073220865955516936053307972551811354915974953502611595561432138406072587814144715094933491949634016143347936046316461046603815184547448500356709747751240174841833572042746076994688103762014887289093164358500713894649408941442423277422140921157781154756929793244704749906604162103591905158114452749907312989913112273170187103589915NCBITaxon:37296103609833418710491130798133679531640729946710340157863033373306383011306413992530642363650595478854698134317103372994044586207340115357111952965261174553111471613386541313166343055213231201798360253237993512993850573163176426197940115134584457726787286232193348621160791392227983516811961255228149539322116761757312108077213487999948861814084341033519963218496209913924882097209620953304393755699286266447NCBITaxon:2697049339862436941103121958832050920290610997586448643249952529724634389427863789426256737863912334355141998349317998686554055343529148654514712454661042986357124679151641358884019373345087718238039129039129529719748693102868899951337310548771251632138985076255470119380625631886571303443516558658862238NCBITaxon:44664946454636493NCBITaxon:11071142800628835649114151678458810874391459438543311167810778543144080439334NCBITaxon:110829534693977382189931822690035128145953399668695954186942003089544869724436620665639545300558623355180426430869220030228481242642815047514991051029910298414452386262903512089557117008930621864419306157558413101653823525577223994636010636010439947544404237610212790568813377518236355721162296298176771870931281105023824518557966946543186320518240974417394124501288531838891988531911137825591037164735166012615572283313NCBITaxon:101142845031631198573859523343237956026087121346395984275295952664373343232235443142752422430814336195931334565943274169963112223360520121697952528386972864368012187073347472790104081882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