Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Spinach_RPs_70S_topdown.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/ecoli_RPs_70S_topdown.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_40S_topdown.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/spinach_ribosome_70S_bottomup.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_60S_topdown.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/human_ribosome_40S_bottomup.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/human_ribosome_60S_bottomup.prot.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_40S_topdown_top3_high_high_1h_grad.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_60S_topdown_top3_high_high.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_40S_topdown_top3_medium_high.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/EColi_RPs_topdown_top5_medium_high_30min_grad_10_to50_ACN_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_60S_topdown_top3_medium_high_1h_grad_NCE35_dyn_excl13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/human_ribosome_60S_bottomup.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_60S_topdown_top3_medium_high_NCE35.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Spinach_RPs_topdown_top3_high_high_1h_grad_NCE35_dyn_excl60.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_60S_topdown_top3_medium_high_1h_grad_NCE35_dyn_excl60.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_40S_topdown_top3_medium_high_1h_grad_NCE35_dyn_excl13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Spinach_RPs_topdown_top3_high_high_1h_grad_NCE35_dyn_excl13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_60S_topdown_top3_high_high_1h_grad_NCE35_dyn_excl60.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Spinach_RPs_topdown_top3_high_high_1h_grad_NCE30_dyn_excl60.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Spinach_RPs_topdown_top3_high_high_1h_grad_NCE30_dyn_excl13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Spinach_RPs_topdown_top3_medium_high_1h_grad_NCE30_dyn_excl13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Spinach_RPs_topdown_top3_medium_high_1h_grad_NCE35_dyn_excl13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_40S_topdown_top3_medium_high_1h_grad.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_40S_topdown_top3_high_high.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/EColi_RPs_topdown_top5_high_high_10min_grad.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_60S_topdown_top3_medium_high.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/human_ribosome_40S_bottomup.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_40S_topdown_top3_high_high_1h_grad_NCE35_dyn_excl13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/EColi_RPs_topdown_top5_high_high_30min_grad_10_to50_ACN.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/spinach_ribosome_70S_bottomup.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_40S_topdown_top3_high_high_1h_grad_NCE35_dyn_excl60.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Spinach_RPs_70S_topdown.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/ecoli_RPs_70S_topdown.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/spinach_ribosome_70S_bottomup.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_40S_topdown.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/human_ribosome_40S_bottomup.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/Human_RPs_60S_topdown.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881/human_ribosome_60S_bottomup.pep.xmlprimaryOK200002990a.j.r.heck@uu.nlSem TamaraMass SpectrometryTop-down proteomicsShotgun proteomicsNot availableRibosomeTop-downBottom-uphttp://www.ebi.ac.uk/pride/archive/projects/PXD008881For bottom-up, ribosome preparations were reduced with 5 mM DTT at 56 °C for 30 minutes and alkylated with 15 mM iodoacetamide at room temperature for 30 minutes in the dark. The excess of iodoacetamide was quenched by adding 5 mM DTT. Digestion of intact proteins was performed at 37 °C with Lys-C for 4 hours followed by overnight digestion with trypsin at an enzyme-to-protein-ratio of 1:100 (w/w). All proteolytic digests were desalted, dried and dissolved in 40 µL of 0.1% FA prior to LC-MS/MS analysis. For top-down LC-MS/MS experiments, ribosomal proteins and ribosome associated proteins were separated from the ribosomal RNA by glacial acetic acid precipitation and buffer exchanged into 0.1% FA.PrideNot availabledimethylated residuephosphorylated residueiodoacetamide derivatized residuemonomethylated residuemonohydroxylated residueacetylated residueBottom-up data was searched with Byonic (Protein Metrics) node in Thermo Proteome Discoverer software (version 2.2.0.388). Following parameters were used for data searches: precursor ion mass tolerance, 10 ppm; product ion mass tolerance, 20 ppm; fixed modification: Cys carbamidomethyl; variable modification: Met oxida-tion. Enzymatic specificity was set to trypsin. Automatic searches for top-down LC-MS/MS raw data were made in Thermo Proteome Discoverer software (version 2.2.0.388) with use of ProSightPD nodes for Medium-High and High-High experimental workflows. Parameters for Medium-High method were set as follows. ReSpect parameters: precursor m/z tolerance – 0.2 Th; relative abundance threshold – 10%; precursor mass range – 5-100 kDa; precursor mass tolerance – 30 ppm; charge state range – 5-100. Xtract parameters: signal-to-noise (S/N) threshold – 3; m/z range – 200-2000 Th. Absolute mass search parameters: precursor mass tolerance – 500 Da; fragment mass tolerance – 10 ppm. For High-High searches ReSpect parameters were not defined, instead Xtract with identical parameters was used to deconvolute spectra in both full MS and dd-MS/MS scans. Final search results are merged for each ribosome purification.ProteomicsAlbert J. R. HeckQ Exactive HFPARTIALBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the NetherlandsHomo Sapiens (human)Escherichia ColiSpinacia Oleracea29950687 van de Waterbeemd M, Tamara S, Fort KL, Damoc E, Franc V, Bieri P, Itten M, Makarov A, Ban N, Heck AJR. Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass spectrometry methods. Nat Commun. 2018 9(1):2493 10.1038/s41467-018-04853-xs.tamara@uu.nlBiologicalUtrecht UniversityNetherlandsBiomolecular mass spectrometry has matured strongly over the past decades and has now reached a stage where it can provide deep insights into the structure and composition of large cellular assemblies. Here, we describe a three-tiered hybrid mass spectrometry approach that enables the dissection of macromolecular complexes in order to complement structural studies. To demonstrate the capabilities of the approach, we investigate ribosomes, large ribonucleoprotein particles consisting of a multitude of protein and RNA subunits. We identify sites of sequence processing, protein post-translational modifications, and the assembly and stoichiometry of individual ribosomal proteins in four distinct ribosomal particles of bacterial, plant and human origin. Amongst others, we report extensive cysteine methylation in the zinc finger domain of the human S27 protein, the heptameric stoichiometry of the chloroplastic stalk complex, the heterogeneous composition of human 40S ribosomal subunits and their association to the CrPV, and HCV internal ribosome entry site RNAs.Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass spectrometry methods.van de Waterbeemd Michiel M, Tamara Sem S, Fort Kyle L KL, Damoc Eugen E, Franc Vojtech V, Bieri Philipp P, Itten Martin M, Makarov Alexander A, Ban Nenad N, Heck Albert J R AJRliquid chromatography tandem mass spectroscopy, Hac-1/Dark, E-260, Dapaf-1/HAC-1, determination, Vinegar, DmelCG6829, ribosomal RNA, Gene, CH3-COOH, Ribosomal Protein, Essigsaeure, protein, splitted from, Apaf-1, protein-containing complex, LC-MS-MS, Buffer, Dark/Hac-1/dApaf1, protein polypeptide chains, Methanecarboxylic acid, Hac1, Dark/Hac-1/dApaf-1, E260, reduced, polypeptide chain, Glacial Acetic Acid, subnumerary, LC-MSMS, Gene Products, Apaf1, tiny, protein aggregate, present in fewer numbers in organism, ARK, CH3CO2H, proportion, Ribosomal, LCMSMS, hac-1, weight-weight percentage, dapaf-1, beta-Trypsin, hypoplasia, Acetic Acid, arc, dapaf, proteins, dark, decreased number, Acetic Acid Glacial, ark, T1, ribosomal ribonucleic acid, decreased, DARK, discontiguous, MeCO2H, ethoic acid, dApaf1, associated, dArk, buffer., Dapaf-1, 2-iodo-, ratio, small, LC-MS2, dark/dapaf-1/hac-1, AcOH, mass percentage, D-Apaf-1, protein complex, dApaf-1, Trypsin/K, Proteins, anon-53Fa, LC-MS/MS, l(2)SH0173, apaf1, APAF1, LC/MS/MS, Glacial, Temperatures, native protein, INSDC_feature:rRNA, HOAc, natural protein, divided_from, Hac-1, Protein, chemical analysis, Dark, membrane bound ribosome, Dark/Apaf-I, CG6829, Acetic acid, free ribosome, dark/hac-1/dapaf-1, Ribosome, Ethylic acid, underdeveloped, ribosomal protein, E 260, proportionality, Tripcellim, Dark/Dapaf-1/HAC1, INSDC_qualifier:unknown, rate, acide acetique, hac1, beta Trypsin, dApaf-1/DARK/HAC-1, w/w, Protein Gene Products, dapaf-1S, Gene Proteins, Trypure, apaf-1, dapaf-1L, MeCOOH, ACETIC ACID, Acetamide, liquid chromatography-tandem mass spectroscopy, Isolation of Nuclei TAgged in specific Cell Types, quotient, liquid chromatography tandem mass spectrometry, INS No. 260, assay, acetic acid, dAPAF-1, Ethanoic acidMass Spectrum Analysis, Mass Spectrum, advanced, lifespan, Procedures, Analyses, entire lifespan, entire life cycle, life, Spectrometry, Methodological, procedures, Spectrum Analyses, Procedure, Methodological Study, Spectrum Analysis, early, Spectroscopy, Study, Techniques, Methodological Studies, Method, precocious, Mass, Studies, Analysis, techniques, methodology., Technique, Mass Spectrum Analyses, Mass Spectroscopyliquid chromatography tandem mass spectroscopy, IPP2A2, Bru, Hgfr, Raw, primitive node, 5730555F13Rik, ribosomal RNA, protein, 5730420M11Rik, protein polypeptide chains, L-Isomer Methionine, Personal, Drug Tolerance, Methionine, dorsal marginal zone, HGFR, node, Software Engineering, protein aggregate, Computer Program, WMS, Svc, SET, LCMSMS, kDa, MUB3_18, TAF-I, M, Open, phosphatase 2A inhibitor I2PP2A, Computer Programs and Programming, proteins, MUB3.18, DmelCG4299, set, IGAAD, DmelCG10574, Hensen node, ppm, Hensen's node, RG7MT1, GPHYSD2, Racemethionine, z, embryo organizer, drug tolerance, SGS, LC-MS2, phapii, proto-oncogene c-Met, Noise, PRO, immune system tolerance, 2-Amino-4-(methylthio)butyric acid, LC-MS/MS, Pollution, StF-IT-1, Th, 9130215G10Rik, CYS, Source Softwares, results, Software Tools, Programs, ACMICD, Program, Computer Applications, Computer Applications Software, Computer Applications Softwares, Immunologic Tolerance, Henson's node, Methionin, methionine, Softwares, Hmet, and GLY protein 2, parent ion, HGF receptor, HGF/SF receptor, and GLY protein 1, Software Applications, HLA-DR-associated protein II, DI-2, Source Software, I-2Dm, Dignity, MASS, CG4299, hCMT1c, inhibitor of granzyme A-activated DNase, c-Met, beta Trypsin, experimental procedures, I-2PP1, Applications, Self Tolerance, TAF-IBETA, precursor ion, Tolerance, liquid chromatography-tandem mass spectroscopy, DMZ, TAF-Ibeta, Noise Pollution, Liquimeth, i2pp2a, Computer Software Applications, 2-amino-4-(methylsulfanyl)butanoic acid, Respect, experimental, L Isomer, FBN, Computer, precursor, protein-containing complex, thomson, PHAPII, LC-MS-MS, mass-to-charge ratio, method, HGF, polypeptide chain, template-activating factor I, ECTOL1, method used in an experiment, LC-MSMS, L-Methionine, F15E12.6, Del(8)44H, RCCP2, Pedameth, Application, shield, Open Source Softwares, F15E12_6, methods, nodus primitivus, scatter factor receptor, Software Application, experimental section, ipp2a2, beta-Trypsin, Open Source Software, 2pp2a, Spemann's organizer, AUTS9, CG10574, Computer Software Application, OCTD, 2-amino-4-(methylthio)butanoic acid, 2PP2A, Tools, 10^[-6], taf-ibeta, stem node, Personal Respect, dSET, dSet, organizer, AI838057, metionina, Applications Software, Open Source, data, Computer Software, DL-Methionine, protein complex, igaad, embryonic organizer, L-Isomer, Tool, Software Tool, LC/MS/MS, native protein, Immune Tolerance, natural protein, I-2PP2A, Protein, Dm I-2, Spemann Mangold organizer, I2PP2A, MFS1, membrane bound ribosome, Software, WMS2, ATCMPG1, MET, Met, ATCMPG2, Ribosome, Col4a-1, nodal stem, tyrosine-protein kinase Met, Engineering, Tripcellim, Noises, plan specification, Computer Programs, Trypure, free ribosome., dSET/TAF-Ibeta, 2610030F17Rik, Applications Softwares, SSKS, alpha-amino-gamma-methylmercaptobutyric acid, embryonic shield, liquid chromatography tandem mass spectrometry, Par4, AA407739, 5730455C01Rik, SF receptor, Immunological Toleranceliquid chromatography tandem mass spectroscopy, LC-MS2, 80S ribosome, spinach, human being, cou, Procedures, Spinacia oleracea L, Spinacia oleracea0, Effects, Proteins, LC-MS/MS, Longterm Effect, Gene, Ribosomal Protein, Procedure, Tl3, Tl2, Long Term, LC-MS-MS, period, Techniques, LC/MS/MS, Lr, Method, LC-MSMS, Protein, Long Term Effects, Gene Products, Studies, Low, techniques, Effect, Technique, Spinacea oleracea, Ribosomal, me75, LCMSMS, Longterm, 70S ribosome, proteins, Methodological, procedures, Long-Term, spinach oleracea, Spinach, man, Methodological Study, D17Mit170, human, Longterm Effects, Protein Gene Products, Study, Gene Proteins, Methodological Studies, liquid chromatography-tandem mass spectroscopy, Bra, T1., liquid chromatography tandem mass spectrometry, Long-Term Effect, time, Long-Term Effects, methodologybig, Internal Ribosome Entry Site., Ribonucleic, MPS1, human being, Cysteine Hydrochloride, conformation, H-PAST, Pflanze, developmental stage, post-transfusion hepatitis non A non B virus, Mbp1, Ribosomal Protein, stalk, protein, Spectrum Analyses, protein-containing complex, composed of, froggy, Gyltl1a, viridiplantae, Zinc Finger Motifs, large, protein polypeptide chains, ribonucleoprotein, etc) (Spanish, exact), polypeptide chain, Associations, Genetic heterogeneity, Mass, Gene Products, Analysis, Half-Cystine, PAST, protein aggregate, myd, Non Polyadenylated, Mass Spectroscopy, RNA Gene Products, Mass Spectrum Analysis, plantae, Ribonucleoprotein, human hepatitis C virus HCV, Ribosomal, Analyses, MDDGB6, L Cysteine, Zinc Finger Motif, Mbp-1, composition, Fingers, proteins, sorophore, man, culm, Non-Polyadenylated RNA, BPFD#36, hepatitis C virus HCV, great, Zinc, Zinc Finger DNA Binding Domains, Motifs, HPAST1, stage, Half Cystine, relational structural quality, Methylations, 柄 (Japanese, Dissections, RNA, human hepatitis C virus, gyltl1b-b, ribose nucleic acid, Zinc Cysteinate, protein complex, axis, Proteins, ribonucleic acids, RNP, RNS, compositionality, terciario, Spectrum Analysis, RNA-protein complex, Spectroscopy, native protein, natural protein, Zinc Finger DNA-Binding Domains, MDDGA6, yeast nucleic acid, mKIAA0609, Ribonukleinsaeure, Protein, sequence, pentosenucleic acids, Ribonucleic acids, methylation, HCV, Mass Spectrum Analyses, fg, Mass Spectrum, deep, ribonucleic acid, Acid, Ribosome, tallo de alto orden (secundario, gyltl1b, distinct, content, Non Polyadenylated RNA, mdc1d, Heterogeneity, Spectrometry, expanded, Non-Polyadenylated, Plant, protein-RNA complex, L-Cysteine, MPS-1, Ribonucleic Acid, stipe (related), PAST1, S27, S-27, primary structure of sequence macromolecule, human, Zinc Finger, MDC1D, Finger, enr, enlarged, structure, Hepatitis C, Motifliquid chromatography tandem mass spectroscopy, LC-MS2, LC-MS/MS, liquid chromatography tandem mass spectrometry, LC-MS-MS., LC/MS/MS, LCMSMS, liquid chromatography-tandem mass spectroscopy, LC-MSMS299trueBottom-up and top-down LC-MS/MS of ribosomal purificationsRibosomal purifications of spinach 70S ribosome and human 40S and 60S ribosomal subunits were analyzed with bottom-up LC-MS/MS to identify proteins comprising purified complexes as well as co-purified proteins. In order to assess and quantify proteoforms of ribosomal proteins in ribosomal purifications we employed top-down LC-MS/MS techniques with optimized methods, wherein apart from standard parameters (e.g. collision voltage and dynamic exclusion time) high-resolution and low-resolution were alternately employed in full MS mode.2018-06-262018-02-07PXD0088814466449917558258038804333029535851114512067811080612461962461972842188090320637522995811363350906836351524260275838538791187947398007186801NCBITaxon:1280572307111430118726355557146835145262364915084727467073641135461388580358232903138886915216778688953997844751870435112329412624696928123977133103430436548135833346079251981836592198131432059655819476214936329572637371922226938124781866132838828139558434513586722294914081691804541619341390363453058611204162326446071121041170612744321085956149636746941283166694369455972945281132081003923751463308793694478447517420654220896431744712704459741194669400667145458793299352932010198126469041066114575512165994048339251216597216595160621213312831282128033952321832172920435315227794428112454349825741948147871720309498019752555536231439841356742951053948824261914548140479NCBITaxon:21575515343989903712285775975320596876231935163098005888455818178081736309431796989361604885661409859566416242515059071187021187053204963063863235554320466322261866331299456586068845351216129567171360441001602358700264203694569410167634102107612773511316582150480227933428146100293578617463313252911187002157641809699745774449156666754760445246767561917265181301941119294396336641136336721751117645102398709NCBITaxon:40559325823590927883965264982174982163472565691569387224982116544872056971666905108944835525663661410NCBITaxon:49328715568910245980875741710005894526808732287535311561263730069303911415542193255521872687238724872987271235987555295476242231414654773055180066556484198822445299767446982510216998231174673897641041051155744689444474153NCBITaxon:50557960167767446851000364960065349118226370824854801299114578458359741276473194439137086326547876569835566369838192011164034624136810893161001428099612NCBITaxon:548681NCBITaxon:58205464143077479464098609958652283196627290587668960596061572951372218759446708756227086171101335483309034974129829387834306154319474703342494729859610896158865597464998703920211107287827215407821190857075298352061894804189853502471587796275630411902528674636046147964696443759391462472746128746011345062534526591972219638191552638588596NCBITaxon:615750521105172375059576131302409263419610526419407722774694163481123869NCBITaxon:2352317036125061747679274596153840052573131368626979686662990346941713443463603475153632964404210867310185109760635586331496161286287966927294351338968583551865368595968912801726071018568729411009118696141633316780781186941436183NCBITaxon:6191118698840231719118691443906NCBITaxon:102982100071257118387279694836419080218787870384766455347095145122578656870861822607046183260705260707NCBITaxon:780NCBITaxon:1502451516658457702921158612184520928574707856100713370565295076192126385454195NCBITaxon:30555044573915383379913998192875NCBITaxon:10239NCBITaxon:714282912680631309570448300641751585923810555241590841228233446900815025754122810451930174830085210977970671356225833136185509924NCBITaxon:5693826883064052779617946995833424090615101515NCBITaxon:631240674482428130767070876138370828167728503515819901195195337024924053608881756261583558333760212671928532177357267119350184645177299808616787917743737111049056371215348112153239576769014726647091317047571256274579031133355085962859634792491308126387121203511082332648373153214693NCBITaxon:9615383233185862607991412622002981314131349376083334111497019281239655833321018413856959111006581237561275045426662551519788749200375036009412934971530094114903524725175014012573983649908515151438992NCBITaxon:11552374539883747445446415259311792866432359839062887051580119459932716011497861603293338922853422999810894535936457428412754121536024271831627713512251179948875058NCBITaxon:1040724271550277916797181673871349130627415895521010306502780291595412629158515849327159570210022633889128768913558893094514647920312421641332803828035192843436909398580894621080348179392118259028788929502745609906901749342725632273218903829286757221247190400727NCBITaxon:5796537457114311233521140206411981087135547714245072295337969835552222129167141262161154293157546139622421392139077020114319338293550833292307411292211484896160106772526499975744727580240550717629968335366581214053576229412816078395763206672400540296543282311843183224659620575549922957575759284593936053307972551811354970667143673353502611595564442143213818492231124060721054211109697622611117957529818184319219496340161433479866265281046603815181044214547448500796267825356709747731751351240565050NCBITaxon:1313607699389424688795588012014887519795962141140822414082234219728909101412285316435186763185431520767737677764940894144242327742NCBITaxon:17732140922088931157781154756208895929793244704207559101177499067499073129899136645112273231663170187367451011610358991513284262658721036098334139269611307981336795358893164071034015303337330158191306413992530688391033721233681994044586449811535765261111471612249111313166343053120175549935129938505779983176669642688451979401151345842157535326787286238621115104160791298315392227468911983511255228562532326604691361651056121491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