Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_17.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_7.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_13.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_6.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_11.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_18.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_14.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_11.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_8.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_18.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_15.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_1.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_4.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_15.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_1.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_3.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_20.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_20.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_12.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_2.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_5.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_12.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_5.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_17.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_19.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_6.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_7.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_9.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_10.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_2.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_3.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_13.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_10.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_9.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_8.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_16.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_4.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_16.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_19.wiffftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/P160264_14.wiff.scanftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008919/F16FTSCCKF1178_I-ZpNCk003_rep1.datprimaryOK200002460jfgui@ihb.ac.cnYaojun ZhuMass SpectrometryShotgun proteomicsNot availableGibel carpTsdSemen proteomicsGsdItraqhttp://www.ebi.ac.uk/pride/archive/projects/PXD008919SemenSemen samples (each sample 200 μl) from three males of GSD and three males of TSD were collected in propagation season. Total proteins of each sample were extracted and quantified by Bradford Assay and SDS-PAGE analysis. Then, the proteins were digested using Trypsin Gold (Promega) after being diluted by 100mM triethylamine borane. Then peptides were labeled using iTRAQ Reagent8-plex Kit (AB SCIEX) according to the manufacturer’s protocol. The labeled peptides with different reagents were combined and desalted with a Strata X C18 column (Phenomenex) and vacuum-dried. Subsequently, the peptides were separated on a Shimadzu LC-20AB HPLC Pump system and reconstituted with buffer A (5%ACN, 95% H2O, pH 9.8) to 2 ml. After that, they were loaded onto a column and separated at a flow rate of 1 ml/min with a gradient of 5% buffer B (5% H2O, 95% ACN, pH 9.8) 10 min, 5-35% buffer B for 40min, 35-95% buffer B for 1 min, 95% buffer B for 3 min, and decreased to 5% within 1 min before equilibrating with buffer B for 10 min. 20 fractions were collected by measuring absorbance at 214 nm. Peptides of each fraction were resolved in buffer A (2% CAN, 0.1% FA) and centrifuged at 20,000 g for 10 min. Then, a LC-20AD nano-HPLC instrument (Shimadzu, Kyoto, Japan) was used to separate the supernatant. A linear gradient from 8% to 35% of buffer B (2% H2O and 0.1% FA in ACN) was run at 300 nL/min to elute the peptides in 35 min, followed by ramping up to 60% in 5 min, up to 80% for 5 min, and finally returned to 5% in 0.1 min and equilibrated for 10 min. TripleTOF analysis was performed with a TripleTOF 5600 System (SCIEX, Framingham, MA, USA). Data was acquired with the following MS conditions: ion spray voltage 2.3kV, curtain gas of 30, nebulizer gas of 15, and interface heater temperature of 150°C. The whole data acquisition used high sensitivity model and the accumulation time for MS1 is 250ms, and the mass ranges was from 350 to 1500 Da. 30 production scans were collected if they exceeded a threshold of 120 counts per second and with charge-state 2+ to 5+. Dynamic exclusion was set for 1/2 of peak width (12s), the collision energy was adjusted to all precursor ions for collision-induced dissociation and the Q2 transmission window for 100Da was 100%. The raw MS/MS data was converted into MGF format by ProteoWizard tool msConvert, the exported MGF files were searching using MASCOT version 2.3.02 (Matrix Science, London, United Kingdom) and at least one unique peptide was necessary for the identified protein. The Mascot search parameters included: trypsin as enzyme, fragment mass tolerance was set to 0.1Da, mass values were set to monoisotopic, oxidation (M) and iTRAQ8plex (Y) as variable modification, peptide mass tolerance was set to 0.05Da, fixed modifications were set to iTRAQ8plex (N-term), iTRAQ8plex (K) and Carbamidomethyl (C). Three biological replicates were indispensable for iTRAQ data analysis. For protein quantification, automated software named IQuant had been used. The PSMs (peptide-spectral match) were pre-filtered at a PSM-level FDR (false discovery rate) of 1% for assessing the confidence of peptides. In order to control the rate of false-positive at protein level, FDR of assembled proteins will be estimated (protein-level FDR <= 0.01). Proteins with 1.2 fold change and Q-value less than 0.05 were determined as differentially expressed proteins (DEPs).PrideNot availableNo PTMs are included in the datasetGene Ontology (GO) database (http://www.geneontology.org/), Clusters of Orthologous Groups of proteins (COG) database (http://www.ncbi.nlm.nih.gov/COG/), Kyoto Encyclopedia of Genes and Genomes (KEGG) database (http://www.genome.jp/kegg/pathway.html) were used for basic bioinformatics analysis. Overrepresentation analyses were performed in the Reactome database (https://reactome.org/) using up-regulated DEPs and down-regulated DEPs. The P-value indicated the statistical significance of each hit pathway, the false discovery rate (FDR) was calculated for estimating the false positives via Benjamini-Hochberg approach in Reactome, All DEPs were used to perform KEGG pathway enrichment analysis using cluster profiler in R via Fisher's exact test, P < 0.05 were considered as statistical significance.ProteomicsJian-Fang GuiTripleTOF 5600PARTIALInstitute of Hydrobiology, Chinese Academy of SciencesCarassius Gibelio29866041 Zhu YJ, Li XY, Zhang J, Li Z, Ding M, Zhang XJ, Zhou L, Gui JF. Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish. BMC Genomics. 2018 19(1):437 10.1186/s12864-018-4823-615071309205@163.comBiologicalIHB,CASChina<h4>Background</h4>Coexistence and transition of diverse sex determination strategies have been revealed in some ectothermic species, but the variation between males caused by different sex determination strategies and the underlying mechanism remain unclear. Here, we used the gynogenetic gibel carp (Carassius gibelio) with both genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) strategies to illustrate this issue.<h4>Results</h4>We found out that males of GSD and TSD in gibel carp had similar morphology, testicular histology, sperm structure and sperm vitality. However, when maternal individuals were mated with males of GSD, sperm nucleus swelling and fusing with the female pronucleus were observed in the fertilized eggs. On the contrary, when maternal individuals were mated with males of TSD, sperm nucleus remained in the condensed status throughout the whole process. Subsequently, semen proteomics analysis unveiled that DNA replication and gene expression-related pathways were inhibited in the sperm from males of TSD compared to males of GSD, and most differentially expressed proteins associated with DNA replication, transcription and translation were down-regulated. Moreover, via BrdU incorporation and immunofluorescence detection, male nucleus replication was revealed to be present in the fertilized eggs by the sperm from males of GSD, but absent in the fertilized eggs by the sperm from males of TSD.<h4>Conclusions</h4>These findings indicate that DNA replication and gene expression-related pathways are associated with the distinct sperm nucleus development behaviors in fertilized eggs in response to the sperm from males of GSD and TSD. And this study is the first attempt to screen the differences between males determined via GSD and TSD in gynogenetic species, which might give a hint for understanding evolutionary adaption of diverse sex determination mechanisms in unisexual vertebrates.Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish.Zhu Yao-Jun YJ, Li Xi-Yin XY, Zhang Jun J, Li Zhi Z, Ding Miao M, Zhang Xiao-Juan XJ, Zhou Li L, Gui Jian-Fang JFWater, CPD photolyase activity, type 1, ion, Raw, PhrB photolyase activity, progressive dementia, Ass-1, Irip, Tay-Sachs disease, BOUND WATER, SDH, oxidane, NAALAdase, Long Term, WATER, HOH, 5730420M11Rik, SDS, Polypeptides, NAALADase I, KL receptor activity, protein polypeptide chains, FOLH, triethylamine sulfite (1:1), Drug Tolerance, B1, SCO5, fold, C530001K22Rik, 3, SCO1, germacrene A synthase activity, Software Engineering, NUP96, Analysis, outer pigmented layer of retina, Gsfsow3, epithelium, WMS, C79691, B, somitogenic mesoderm, amyloidosis, SET, Divorced, triethylamine sulfite (2:1), F, C79325, B variant GM2 gangliosidosis, Analyses, Membrane glutamate carboxypeptidase, N, phosphatase 2A inhibitor I2PP2A, Kitl, proteins, Divorces, W, T6G21.3, Mast cell growth factor, triethylamine phosphate, triethylamine maleate (1:1), HPLC, SUPPRESSOR OF AUXIN RESISTANCE 3, AI047805, DmelCG4299, Lccp, PA1, allergic reaction, set, adult type, pigment epithelium of retina, decreased, column, scientific observation, ACN, sample, D1, Acn, n, pigmented retina epithelium, Bs, drug tolerance, SGS, ejaculate, Stars, Gerstmann-Straussler type, Bonin Islands, unsegmented mesenchyme, immune system tolerance, Longterm Effect, somatomedin, glycogen metabolism disorder, IBP1, GAST, SH2-B PH domain-containing signaling mediator 1, not genetically inherited, collisionally activated dissociation, CID, Software Tools, ACMICD, PBT, Computer Applications, H2O, Seminal Plasma, Dm1, Prnp-related, Computer Applications Software, Gerstmann-Straussler-Scheinker disease, dipyrimidine photolyase (photosensitive), hexa deficiency, 6-trans-farnesyl-diphosphate diphosphate-lyase (germacrene-A-forming) activity, pbt, Software Applications, HLA-DR-associated protein II, instrument, DI-2, Source Software, I-2Dm, FGCP, triethylamine phosphonate (1:1), Stem cell factor, subacute spongiform encephalopathy, proportionality, pooled, rate, MASCOT, inhibitor of granzyme A-activated DNase, GSD, STAT5, study protocol, beta Trypsin, Tay-Sachs, I-2PP1, Pro-rich, seminal fluid, stem cell factor, Applications, TAF-IBETA, Self Tolerance, GM2-gangliosidosis, TAF-Ibeta, STARS, inborn glycogen storage disorder, Gb, krk1, Computer Software Applications, F10B6_15, breadth, Clo, Effects, False, Slf, SLF, encephalopathy, and amyloid deposits in CNS, 2-trans, precursor, protein-containing complex, body system, F10B6.15, CG7826, deoxyribodipyrimidine photolyase activity, agua, stratum pigmentosum retinae, period, sensitive, Gerstmann-Straussler disease, Gene Products, CG7835, CG42273, B variant GM2-gangliosidosis, system, encephalopathy subacute spongiform Gerstmann-Straussler type, Application, glycogenoses, amyloidosis cerebral with spongiform encephalopathy, Open Source Softwares, proportion, anatomical systems, Acinus, Longterm, Software Application, beta-Trypsin, Open Source Software, F23A5.3, 4733401P19Rik, 2pp2a, Sciences, Long-Term, ions, study assay, U.K., CG10574, Computer Software Application, acinusL, mKIAA0989, Con, 2PP2A, Tools, acinusS, dSET, dSet, blz, peptidos, with spongiform encephalopathy, triethylamine phosphate (1:1), PTHB1, measuring, proto-oncogene c-Kit, adult chronic type, inborn glycogen metabolic process disorder, hematopoietic growth factor KL, Proteins, triethylamine sulfate, and amyloid deposits in the central nervous system, buffer, (+)-(10R)-germacrene A synthase activity, mKIAA0670, Tool, polypeptide, TSD, Temperatures, native protein, Immune Tolerance, I-2PP2A, KIT ligand receptor activity, C18, Subacute spongiform, chemical analysis, Long Term Effects, Dm I-2, MFS1, segmental plate, triethylamine acetate, Sh2bpsm1, glycogenosis, XKrk1, AU020952, hypersensitivity, RPE, Col4a-1, Protein., Separations, Striated muscle activator of Rho-dependent signaling, SWDS, CD117, retinal pigment epithelium, Longterm Effects, Folate hydrolase 1, ME-IV, p. pigmentosa retinae, plan specification, Computer Programs, Gene Proteins, Applications Softwares, C-Kit, Ssm, MGF, Mgf, variant B1, xkl-1, quotient, deoxyribonucleate pyrimidine dimer lyase (photosensitive), [OH2], Data Analyses, Immunological Tolerance, SH2-Bb, IPP2A2, Bru, determination, instrument configuration, Somatomedin-C, hexosaminidase a deficiency, Xkl-1, protein, triethylamine dinitrate, pigmented epithelium, contrasted, peptide, Gsfsco1, peptido, ClvPrd, BANF, AA408052, Min, 3.4.17.21, glycogen storage disease, protein aggregate, Gsfsco5, present in fewer numbers in organism, Effect, Computer Program, DmelCG42273, SOW3, GM2 gangliosidosis, Svc, SH2-B, peptides, reference sample, TAF-I, 2610036I19Rik, Open, 2610510L13Rik, somatomedin-C, pigmented retina, min, SF, Computer Programs and Programming, mAPC, NAALAD1, Estimated, pseudo-Ab variant, DNA cyclobutane dipyrimidine photolyase activity, Folylpoly-gamma-glutamate carboxypeptidase, sphingolipidosis, ASS, inborn error of glycogen metabolic process, IGAAD, fSAP152, SOLO DANCERS, DmelCG10574, IGF1, triethylamine sulfate (2:1), C16orf53, Mechano growth factor, dihydridooxygen, Soluble KIT ligand, triethylamine hydrobromide, dihydrogen oxide, Sl, GPHYSD2, rare inborn error of glycogen metabolic process, Long-Term Effects, ratio, (+)-germacrene A synthase activity, phapii, IGF-I, PRE, steel factor, PLATEST, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, aqua, Dmel_CG7826, AI836084, StF-IT-1, Tr-kit, pigmented retinal epithelium, Source Softwares, sKITLG, Programs, any method, triethylamine hydrochloride, Program, Ionen, retinal pigment, PSM, Psm, mechano growth factor, Computer Applications Softwares, kl1-A, Immunologic Tolerance, Softwares, KIT, presumptive somite mesoderm, Prostate-specific membrane antigen, GAS, Igf-1, fixed, retinal pigment layer, Dmel_CG7835, Mnb, MNB, parent ion, tyrosine-protein kinase Kit, TAY-Sachs disease, SHEP7, Pteroylpoly-gamma-glutamate carboxypeptidase, mast cell growth factor, FDR adjusted p-value, retinal pigmented epithelium, hydrogen hydroxide, kit, MASS, CG4299, gas, AW124434, acqua, organ system, disease, Kitlg, phr A photolyase activity, KL-1, Gerstmann Straussler Scheinker syndrome, DNA-photoreactivating enzyme, precursor ion, Tolerance, hypersensitivity reaction disease, KITLG, PSMA, Polypeptide, Wasser, i2pp2a, time, Platelets, SH2 domain-containing protein 1B, AW549739, unsegmented paraxial mesoderm, SCF receptor activity, FBN, Gene, FPH2, ACINUS, Computer, photoreactivating enzyme activity, somitomeric mesoderm, GCPII, PHAPII, Buffer, method, Isle of Man, scfr, reduced, template-activating factor I, polypeptide chain, Ms1, subnumerary, ECTOL1, method used in an experiment, N-acetylated-alpha-linked acidic dipeptidase I, stratum pigmentosa retinae, mKIAA1299, Del(8)44H, juvenile, SCFR, Separated, sensitivity, Pro-Mega, cerebral, cerebellar ataxia, SH2B, Fdc, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, MS1, PRECOCIOUS, iones, hexosaminidase alpha-subunit deficiency (variant B), ipp2a2, mGCP, eau, gangliosidosis GM2, decreased number, OCTD, sperm, taf-ibeta, male sterility 1, hypersensitivity reaction, Long-Term Effect, F23A5_3, Controlled, Applications Software, triethylammonium formate, Open Source, DYRK1, data, Controlling, Computer Software, Males, protein complex, 6-trans-farnesyl-diphosphate diphosphate-lyase [(+)-germacrene-A-forming] activity, igaad, Great Britain, N-diethylethanamine, Peptide, GCP2, Glutamate carboxypeptidase II, several forms, stratum pigmentosum (retina), Software Tool, MODIFIER OF SNC1, Cell growth-inhibiting gene 27 protein, CAD, natural protein, water, Protein, I2PP2A, deoxyribonucleic photolyase activity, Dyrk1, Vacuums, connected anatomical system, Hex A pseudodeficiency, Software, WMS2, hexosaminidase A deficiency, Plasma, prion dementia, Separation, c-KIT, 10^[-9], PH and SH2 domain-containing signaling mediator, hypersensitive, MALE STERILITY 1 PROTEIN, photolyase activity, CC1, Engineering, Tripcellim, cerebral amyloid angiopathy, CGI-97, sample population, B1 variant, Protein Gene Products, Trypure, glycogen storage disorder, dSET/TAF-Ibeta, c-kit, 2610030F17Rik, Ion, Data, SSKS, c-Kit ligand, AI425885, Peptid, assay, SCF, SCH, AA407739, SeminalSOXDP., Sperm, SOXB2.1, noyau atomique, l(3)LG9, Processes, fishes, 2310014D11Rik, Sry-rDM33, Sry-rDM36, Kern, Y-Chromosome-Bearing Sperm, Atomkern, Agnatha, loD, AA589508, DM63, Sh3md1, C230050L11, horsetail nucleus, TSK5, Spermatozoon, Sry-rDM23, CG5893, nucleo atomico, Y Bearing Sperm, X-Chromosome-Bearing Sperm, nuclei, Gm5098, sperm cell, neuronal nucleus, nucleus atomi, bony fishes, X-Bearing, DM10, nucleus of CNS, noyau, nucleo, X-Bearing Sperms, behavioral response to stimulus, Fish, EG329070, X-Chromosome-Bearing Sperms, Sry-rDM10, Y Chromosome Bearing Sperm, Y-Bearing Sperm, nucleus, sperm, X Chromosome Bearing Sperm, Y-Chromosome-Bearing Sperms, SPERM, associated, fish, Behaviors, FISH, behaviour, Sox70D, ejaculate, single-organism behavior, Y-Chromosome-Bearing, DmelCG5893, X-Bearing Sperm, Sox B2-1, AI256723, DM33, Process, Males, DM36, SH3MD1, Sox70, Acceptance Processes, spermatozoon, Actinopterygi, Acceptance Process, Temperatures, ray-finned fishes, DM23, nervous system nucleus, SOX70D, Cephalaspidomorphi, cell nucleus, AI413738, Y-Bearing, Sry-rDM63, Hyperotreti, Acceptance, X-Chromosome-Bearing, Monorhina, distinct, nucleus of neuraxis, spermatozoid, X Bearing Sperm, neuraxis nucleus, TKS5, Tks5, Osteichthyes, seminal fluid, SOX70, Y-Bearing Sperms, behavioural response to stimulus, SpermsGpi, Gene Ontology Projects, Encyclopedias, Materials, determination, AI461847, False, Proteins, Gene, MF, Amf, Cistrons, Gene Ontology Project, Gene Ontology, mOC-X, Protein, chemical analysis, Gene Products, Project, Genetic Materials, Ontology Projects, Pgi, NK|GPI, Gpi-1, Genetic Material, Data Base, NK, Ontology Project, Gene Ontologies, Ontology, proportion, ORG, Org, F, Ontologies, Genetic, Projects, Genomes, Gpi-1r, Nlk, Gpi-1s, Phi, proportionality, Gpi-1t, chemical analysis., Gpi1-r, Gpi1-s, rate, proteins, Gpi1-t, Protein Gene Products, Gene Proteins, Material, quotient, Cistron, assay, Bglap-rs1, Gpi1s, NK/GPI, ratioprojections, dSetdb1, type 1, egg, single-organism developmental process, l(3)LG9, progressive dementia, Sry-rDM33, hexosaminidase a deficiency, Tay-Sachs disease, Sry-rDM36, bA320F15.2, MST126, Atomkern, C230050L11, responsivity, horsetail nucleus, TSK5, Sry-rDM23, hexosaminidase A deficiency., (2Z)-but-2-enedioate, glycogen storage disease, male, X-Chromosome-Bearing Sperm, GM2 gangliosidosis, Gm5098, sperm cell, B, neuronal nucleus, nucleus atomi, amyloidosis, bony fishes, X-Bearing, DERP5, DM10, B variant GM2 gangliosidosis, Cyprinus cyprio, mirror carp, behavioral response to stimulus, Fish, CG30426, pseudo-Ab variant, CG30422, Sry-rDM10, sphingolipidosis, inborn error of glycogen metabolic process, Y Chromosome Bearing Sperm, Dmsetdb1, SETDB1, adult type, Y-Bearing Sperm, X Chromosome Bearing Sperm, papilla, Y-Chromosome-Bearing Sperms, DmelCG12196, fish, FISH, Sox70D, rare inborn error of glycogen metabolic process, single-organism behavior, ejaculate, RP11-394I23.1, Y-Chromosome-Bearing, Gerstmann-Straussler type, anatomical protrusion, DM33, Process, DM36, lamina, flanges, glycogen metabolism disorder, SetDB1, Acceptance Processes, Dmel_CG30422, Acceptance Process, Seminal Plasma, ray-finned fishes, DM23, Prnp-related, Cephalaspidomorphi, Dmel_CG30426, shelf, Gerstmann-Straussler-Scheinker disease, cell nucleus, hexa deficiency, MCARP, AI413738, Sry-rDM63, X-Chromosome-Bearing, TAY-Sachs disease, dsetdb1, Monorhina, maleate, shelves, subacute spongiform encephalopathy, dSETDB1, GSD, spermatozoid, projection, X Bearing Sperm, ridge, Tay-Sachs, Crap, TKS5, Tks5, seminal fluid, disease, DmSetdb1, C10orf97, Gerstmann Straussler Scheinker syndrome, spine, GM2-gangliosidosis, Sperms, MARP, inborn glycogen storage disorder, Dm-setdb1, CARP, Sperm, SOXB2.1, noyau atomique, Peptidomics, Processes, lamellae, encephalopathy, fishes, 2310014D11Rik, and amyloid deposits in CNS, number, Kern, Y-Chromosome-Bearing Sperm, process of organ, presence, Agnatha, loD, protrusion, lamella, Carp, AA589508, DM63, Sh3md1, AAM70794, Gerstmann-Straussler disease, Spermatozoon, B variant GM2-gangliosidosis, carp, CVARP, CG5893, nucleo atomico, AAF47268 Dm, Y Bearing Sperm, juvenile, encephalopathy subacute spongiform Gerstmann-Straussler type, cerebral, nuclei, glycogenoses, amyloidosis cerebral with spongiform encephalopathy, study, reactivity, cerebellar ataxia, C-193, BcDNA:AT13877, nucleus of CNS, noyau, nucleo, hexosaminidase alpha-subunit deficiency (variant B), X-Bearing Sperms, ridges, gangliosidosis GM2, CG12196, EG329070, X-Chromosome-Bearing Sperms, nucleus, sperm, SPERM, fancy carp, Behaviors, laminae, behaviour, with spongiform encephalopathy, DmelCG5893, X-Bearing Sperm, koi, Sox B2-1, AI256723, adult chronic type, Males, inborn glycogen metabolic process disorder, SH3MD1, anatomical process, Sox70, and amyloid deposits in the central nervous system, spermatozoon, Actinopterygi, development, TSD, several forms, count in organism, Temperatures, nervous system nucleus, SOX70D, Subacute spongiform, my042, ALRP, Hex A pseudodeficiency, Y-Bearing, glycogenosis, flange, organ process, hexosaminidase A deficiency, SOXDP, Hyperotreti, Plasma, Acceptance, prion dementia, dEset, MARP1, cerebral amyloid angiopathy, nucleus of neuraxis, Alrp, neuraxis nucleus, B1 variant, Osteichthyes, male human body, processes, process, glycogen storage disorder, SOX70, Y-Bearing Sperms, Egg, behavioural response to stimulus, variant B1, processus, Cyrpinus carpio, response, quantitative, common carp, Seminal, presence or absence in organismprojections, protein translation, type 1, NMAN, single-organism developmental process, progressive dementia, determination, hexosaminidase a deficiency, Tay-Sachs disease, histology, bA320F15.2, MST126, Atomkern, Readability, responsivity, horsetail nucleus, symptoms, Autonomous Replication, glycogen storage disease, (2Z)-but-2-enedioate, anatomy and histology, male, GM2 gangliosidosis, X-Chromosome-Bearing Sperm, B, sperm cell, amyloidosis, neuronal nucleus, nucleus atomi, X-Bearing, DERP5, Gene Expressions, B variant GM2 gangliosidosis, Cyprinus cyprio, mirror carp, proteins, present in organism, behavioral response to stimulus, pseudo-Ab variant, Autonomous, sphingolipidosis, inborn error of glycogen metabolic process, reaction, Y Chromosome Bearing Sperm, DNA-dependent, adult type, Y-Bearing Sperm, X Chromosome Bearing Sperm, papilla, Y-Chromosome-Bearing Sperms, associated, transcription from bacterial-type RNA polymerase promoter, rare inborn error of glycogen metabolic process, compressed, ejaculate, single-organism behavior, RP11-394I23.1, Y-Chromosome-Bearing, screening, AA673479, protein anabolism, Hint, Gerstmann-Straussler type, anatomical protrusion, protein biosynthetic process, DNA-dependent transcription, Process, Ipk1, lamina, flanges, glycogen metabolism disorder, results, Acceptance Processes, Acceptance Process, Seminal Plasma, HINT, Prnp-related, dense, shelf, Gerstmann-Straussler-Scheinker disease, protein formation, hexa deficiency, cell nucleus, MCARP, PRKCNH1, X-Chromosome-Bearing, TAY-Sachs disease, absence, histopathology, maleate, shelves, subacute spongiform encephalopathy, signs, GSD, spermatozoid, projection, X Bearing Sperm, ridge, Crap, Tay-Sachs, seminal fluid, disease, C10orf97, squashed, Gerstmann Straussler Scheinker syndrome, Replications, protein synthesis, spine, GM2-gangliosidosis, Sperms, bacterial transcription, MARP, Autonomous Replications, DNA, inborn glycogen storage disorder, CARP, transcription, Sperm, noyau atomique, conformation, Peptidomics, lamellae, Processes, encephalopathy, and amyloid deposits in CNS, Gene, Kern, Y-Chromosome-Bearing Sperm, process of organ, protrusion, lamella, Carp, morphology, swelling, Gerstmann-Straussler disease, Spermatozoon, Gene Products, carp, B variant GM2-gangliosidosis, CVARP, nucleo atomico, juvenile, Y Bearing Sperm, DNA Replications, encephalopathy subacute spongiform Gerstmann-Straussler type, cerebral, glycogenoses, nuclei, amyloidosis cerebral with spongiform encephalopathy, cerebellar ataxia, reactivity, study, C-193, anatomy, nucleus of CNS, noyau, nucleo, hexosaminidase alpha-subunit deficiency (variant B), absent from organism, X-Bearing Sperms, ridges, gangliosidosis GM2, Expressions, X-Chromosome-Bearing Sperms, nucleus, sperm, fancy carp, SPERM, species, Expression, Behaviors, laminae, with spongiform encephalopathy, relational structural quality, behaviour, koi, X-Bearing Sperm, compact, cellular transcription, findings, adult chronic type, Males, inborn glycogen metabolic process disorder, anatomical process, Proteins, and amyloid deposits in the central nervous system, spermatozoon, development, TSD, several forms, Temperatures, nervous system nucleus, Subacute spongiform, chemical analysis, Protein, protein biosynthesis, Vertebrata, my042, background, Vertebrate, ALRP, Hex A pseudodeficiency, Replication, Y-Bearing, glycogenosis, flange, organ process, hexosaminidase A deficiency, Plasma, PKCI-1, Acceptance, prion dementia, MARP1, distinct, DNA-templated, cerebral amyloid angiopathy, Alrp, nucleus of neuraxis, Understanding, neuraxis nucleus, introduction, B1 variant, Protein Gene Products, male human body, processes, process, Gene Proteins, glycogen storage disorder, Y-Bearing Sperms, behavioural response to stimulus, vertebrates., variant B1, processus, Cyrpinus carpio, assay, response, common carp, SeminalSOXDP., CARP, Sperm, SOXB2.1, noyau atomique, l(3)LG9, Processes, fishes, 2310014D11Rik, Sry-rDM33, Sry-rDM36, Kern, bA320F15.2, MST126, Y-Chromosome-Bearing Sperm, Atomkern, Agnatha, loD, Carp, AA589508, DM63, Sh3md1, C230050L11, horsetail nucleus, TSK5, Spermatozoon, Sry-rDM23, carp, CVARP, CG5893, nucleo atomico, Y Bearing Sperm, X-Chromosome-Bearing Sperm, nuclei, Gm5098, sperm cell, neuronal nucleus, nucleus atomi, bony fishes, C-193, X-Bearing, DERP5, DM10, Cyprinus cyprio, nucleus of CNS, noyau, nucleo, mirror carp, X-Bearing Sperms, behavioral response to stimulus, Fish, EG329070, X-Chromosome-Bearing Sperms, Sry-rDM10, Y Chromosome Bearing Sperm, Y-Bearing Sperm, nucleus, sperm, X Chromosome Bearing Sperm, Y-Chromosome-Bearing Sperms, fancy carp, SPERM, associated, fish, Behaviors, FISH, behaviour, Sox70D, ejaculate, single-organism behavior, RP11-394I23.1, Y-Chromosome-Bearing, DmelCG5893, koi, X-Bearing Sperm, Sox B2-1, AI256723, DM33, Process, Males, DM36, SH3MD1, Sox70, Acceptance Processes, spermatozoon, Actinopterygi, Acceptance Process, Seminal Plasma, Temperatures, ray-finned fishes, DM23, nervous system nucleus, SOX70D, Cephalaspidomorphi, cell nucleus, MCARP, my042, ALRP, AI413738, Y-Bearing, Sry-rDM63, Plasma, Hyperotreti, Acceptance, X-Chromosome-Bearing, MARP1, Monorhina, distinct, Alrp, nucleus of neuraxis, spermatozoid, X Bearing Sperm, neuraxis nucleus, Crap, TKS5, Tks5, Osteichthyes, seminal fluid, SOX70, Y-Bearing Sperms, C10orf97, behavioural response to stimulus, Sperms, MARP, Cyrpinus carpio, common carp, Seminal246trueSemen of gibel carp - Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fishIn our study, differential male nucleus events and development behaviors were revealed from the fertilized eggs in response to the sperm from males of genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) in gibel carp. When the eggs of maternal fish were fertilized by the sperm from males of GSD, the fertilized egg encountered similar sexual reproduction events and behaviors. However, when the eggs of maternal fish were fertilized by the sperm from males of TSD, a typical process of gynogenesis was observed. To reveal the underlying molecular mechanism of differential sperm nucleus development behaviors in the fertilized eggs, iTRAQ-based quantitative semen proteomics were performed on three semen samples from three males of GSD and three semen samples from three males of TSD 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