Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/mqpar.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/ELITE-RSLC013491.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/ELITE-RSLC000958.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/ELITE-RSLC018974.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/ELITE-RSLC013487.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/ELITE-RSLC013490.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/ELITE-RSLC018972.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/ELITE-RSLC000960.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/ELITE-RSLC018899.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/ELITE-RSLC013486.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/UniProt_ProteomeSet_Mouse_Isoform_BirAFLNc1821.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/MaxQuant_combined_folder.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/MaxQuant_apl_files.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD009159/MaxQuant_index_files.zipprimaryOK200Bettina.Warscheid@biologie.uni-freiburg.deFriedel DrepperAffinity purification coupled with mass spectrometry proteomicsMass SpectrometryAndromedaMaxQuantBioidBiraFlncMyotubehttp://www.ebi.ac.uk/pride/archive/projects/PXD009159Skeletal Muscle FiberMyotubeSILAC-encoded C2 cells transiently expressing BirA* or BirA* FLNc d18-21 were differentiated for 4 days. Differentiation was induced by reduction of the dialyzed FCS content to 2% in the absence of sodium pyruvate. Differentiation medium was changed every 48 h until day 4 and subjected to EPS (4 ms, 10 V, 0.05 Hz) for 4 h prior to cell lysis. To enhance biotinylation, 50 µM biotin (Amresco, Solon, USA) were added to the culture medium 24 h prior to cell lysis. One set of cells expressing BirA* FLNc d18-21 was cultured without adding biotin and served as an additional control. Cell lysis was performed as described by Roux et al. (2012), protein concentrations were equalized using the Bradford assay followed by mixing of samples in a 1:1:1 ratio to a final protein amount of 900 µg per replicate. Coupling of biotinylated proteins to streptavidin Dynabeads (Invitrogen, Karlsruhe, Germany) and subsequent washing steps were performed as described by Roux et al. For tryptic on-bead digestion, samples were washed twice with 50 mM ammonium bicarbonate solution and incubated with sequencing grade trypsin (Promega) in a 1:50 (w/w) ratio for 3.5 h at 42°C and 200 rpm on a thermoshaker. Supernatants were acidified to a final concentration of 1% TFA on ice, split in four equal parts and dried in vacuo.PrideSILACbiotinylated residueiodoacetamide derivatized residueacetylated residueRaw files from LC-MS/MS analyses of BioID assays were processed using Andromeda embedded in MaxQuant 1.5.5.1 and searched against the Uniprot Proteome Set mouse database (release 01.2016, 58,790 protein entries) supplemented with the fusion protein BirA*FlnC d18-21. Trypsin was set as proteolytic enzyme allowing up to two missed cleavages. Carbamidomethylation of cysteine residues was set as fixed modification. N-terminal acetylation, methionine oxidation and phosphorylation at serine, threonine and tyrosine residues were allowed as variable modifications. The precursor mass tolerance for first search was set to 20 ppm and to 4.5 ppm for main search. The mass tolerance of fragment ions was set to 0.5 Da. A FDR of 1% was applied on both peptide (on modified peptides separately) and protein lists and a minimum of one unique peptide was enabled for protein identification.ProteomicsBettina WarscheidLTQ Orbitrap EliteDepartment of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology and BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg im Breisgau, GermanyPARTIALMus Musculus (mouse)friedel.drepper@biologie.uni-freiburg.de32444788 Reimann L, Schwäble AN, Fricke AL, Mühlhäuser WWD, Leber Y, Lohanadan K, Puchinger MG, Schäuble S, Faessler E, Wiese H, Reichenbach C, Knapp B, Peikert CD, Drepper F, Hahn U, Kreutz C, van der Ven PFM, Radziwill G, Djinović-Carugo K, Fürst DO, Warscheid B. Phosphoproteomics identifies dual-site phosphorylation in an extended basophilic motif regulating FILIP1-mediated degradation of filamin-C. Commun Biol. 2020 3(1):253 10.1038/s42003-020-0982-5BiologicalAG WarscheidBiologie IIAlbert-Ludwigs-Universität FreiburgSchänzlestr. 179104 FreiburgGermanyGermanyThe PI3K/Akt pathway promotes skeletal muscle growth and myogenic differentiation. Although its importance in skeletal muscle biology is well documented, many of its substrates remain to be identified. We here studied PI3K/Akt signaling in contracting skeletal muscle cells by quantitative phosphoproteomics. We identified the extended basophilic phosphosite motif RxRxxp[S/T]xxp[S/T] in various proteins including filamin-C (FLNc). Importantly, this extended motif, located in a unique insert in Ig-like domain 20 of FLNc, is doubly phosphorylated. The protein kinases responsible for this dual-site phosphorylation are Akt and PKCα. Proximity proteomics and interaction analysis identified filamin A-interacting protein 1 (FILIP1) as direct FLNc binding partner. FILIP1 binding induces filamin degradation, thereby negatively regulating its function. Here, dual-site phosphorylation of FLNc not only reduces FILIP1 binding, providing a mechanism to shield FLNc from FILIP1-mediated degradation, but also enables fast dynamics of FLNc necessary for its function as signaling adaptor in cross-striated muscle cells.Phosphoproteomics identifies dual-site phosphorylation in an extended basophilic motif regulating FILIP1-mediated degradation of filamin-C.Reimann Lena L, Schwäble Anja N AN, Fricke Anna L AL, Mühlhäuser Wignand W D WWD, Leber Yvonne Y, Lohanadan Keerthika K, Puchinger Martin G MG, Schäuble Sascha S, Faessler Erik E, Wiese Heike H, Reichenbach Christa C, Knapp Bettina B, Peikert Christian D CD, Drepper Friedel F, Hahn Udo U, Kreutz Clemens C, van der Ven Peter F M PFM, Radziwill Gerald G, Djinović-Carugo Kristina K, Fürst Dieter O DO, Warscheid Bettina BFLN-C, Networks, Forms, DmelCG5178, PRKBA, DmelCG7478, act 87E, Akt/PKB, Kinship, DAKT1/PKB, Aminosaeure, Amino acid, protein, AKT1, Act5, phosphorylation, binding site, l(1)G0420, DmelCG12051, protein polypeptide chains, act 88F, Life Cycle, Impacts, Filamin-2, ABP280A, alpha Filamin, Environmental Impacts, protein aggregate, Family Life Cycle, A, rsd, C, D, E, cyt5C, amino acids, Kinship Network, M, 1110055E19Rik, proteins, act88F, PKB|Akt, beta Filamin, RacPK, Environmental Impact, act 42A, n, Ac5C, CG4027, Act, house mouse, Family Life Cycles, DmelCG18290, act87E, PKB alpha, gamma-filamin, l(1)G0330, dakt, Actin/BAP47, Aminokarbonsaeure, Cs, mouse, act, RAC-PK-alpha, l(3)89Bq, beta-Filamin, alpha-amino carboxylic acids, mice C57BL/6xCBA/CaJ hybrid, act42A, 1-Like Proteins, AKT1 kinase, ACTIN, Filamin 1 Like Protein, PKB/Akt, PKB/AKT, AKT/PKB, protein kinase B alpha, dAkt, dAKT, AFFX-Dros-ACTIN_M_r_at, actin, p-Akt, CG7478, CG5178, Filiation, DAkt, CG10067, region, Act57A, l(3)04226, Amino Acid, Acid, DPKB, MFM5, DmelCG4027, positional polypeptide feature, Amino acids, pAkt, FLN2, Fln2, mice, l(1)G0117, actin-binding-like protein, Actin, proto-oncogene c-Akt, FLNC, dAKT/dPKB, PKB/dAKT, beta-actin/Bap47, 280 kDa Actin-Binding Protein, Act57b, muscle element, protein kinase B, l(1)G0486, act79B, Life Cycles, l(1)G0245, immunoglobulin, actin5C, Dakt, l(1)G0009, Filamin Cs, dPKB, Acids, Mouse, RAC-ALPHA, act57b, Act5c, DRAC-PK85, DmelCG4006, l(1)G0010, Act87e, Family Member, region or site annotation, Aminocarbonsaeure, number, ACT5C, CG12051, Gene, Network, alpha-amino acid, protein-containing complex, anon-EST:CL2c12, presence, act57B, CG30294, l(1)G0025, act57A, antibody, Dpkb, Act88f, polypeptide chain, alpha-Filamins, Filamin 1-Like Proteins, actin-12, Gene Products, FLNc, Pulses, 143060_f_at, Gamma-filamin, positional, act5C, ABP-280-like protein, DmelCG10067, Research, ligand, mus79B, Act57, 280 kDa Actin Binding Protein, anon-EST:fe2D2, Act42a, CG18290, AKT, Akt, musculus, DRAC-PK, T10, site, alpha-Filamin, T11, Kinship Networks, MPD4, Family, Act-5C, l(1)G0177, Ifm(3)7, 42A, filamin-2, Family Research, akt, protein complex, act 79B, Filamin 1-Like Protein, Proteins, Phosphorylations, Actin-binding-like protein, DAkt1, function, alpha-amino acids, DAKT1, BEST:RE38067, Mus muscaris, Impact, Family Members, Filamin 1, Merofilamin, Environmental, count in organism, PKB, beta-Filamins, native protein, CWS6, natural protein, Mus, beta-actin, Protein, M32055, PKB-ALPHA, Filamin A, Act42, D-Akt, CG4006, phosphorylation enrichment, Filamin B, Filamin C, ABP-280, 1-Like Protein, opsonin activity, Dakt1, l(1)G0079, serine-threonine kinase Akt, ABPL, 10^[-9], Act88-F, akt1, Merofilamins, dAkt1, ABPA, INSDC_feature:misc_binding, act 57A, INS, DRAC-PK66, act 5C, muscle, Amino, mus87E, Protein Gene Products, Gene Proteins, dAkt/PKB, dakt1, presence or absence in organism., binding_or_interaction_site, Families, Act(88F), PKBalpha, Filamin 1-Like, B cell receptor activity, Environments, BAP47, dAKT1, RAC, Rac, quantitative, Relatives, Filamin, Bap47, ABP-L, Actin5C, thymoma viral proto-oncogeneFLN-C, sodium salt, 4-d]imidazol-4-yl)pentanoic acid, Deacura, IL1BC, vitamin B7, Biotine, DrIce, DrICE, CASP-1, Medebiotin, CG7788, Gene, crice, protein, Vitamin H, protein-containing complex, composed of, Strepavidin, DRICE., culture filtrate, protein polypeptide chains, 5-[(3aS, polypeptide chain, Biotin, 4beta, Gene Products, 6aR)-Hexahydro-2-oxo-1H-thieno[3, Filamin-2, ABP280A, protein aggregate, 6aalpha))-, Il1bc, Gelfert, FLNc, pyruvic acid, Pro-Mega, Gamma-filamin, Biotin Hermes, proportion, ABP-280-like protein, reference sample, Coenzyme R, absent from organism, (3aS, weight-weight percentage, 1110055E19Rik, cis-Hexahydro-2-oxo-1H-thieno(3, beta-Trypsin, PCE-2, biotina, composition, biotine, proteins, BIOTIN, study assay, IL-1BC, Solution, Biotinylations, 4-d]imidazole-4-valeric acid, Hermes, Caspase-1 subunit p20, ICE, (+)-cis-Hexahydro-2-oxo-1H-thieno[3, scientific observation, biotinum, MPD4, Controlled, ratio, Medobiotin, measuring, gamma-filamin, Controlling, mass percentage, filamin-2, culture medium, bead, Caspase-1 subunit p10, Natriumpyruvat, Rombellin, ice, iCE, drice, caspase 3, protein complex, Proteins, Roche, Actin-binding-like protein, Biotin Gelfert, CE-2, compositionality, 5-(2-oxohexahydro-1H-thieno[3, Cell, 4]imidazole-4-valeric acid, 4S, 4-d)imidazole-4-pentanoic acid, (3aS-(3aalpha, DmelCG7788, 6aR)-2-oxohexahydro-1H-thieno[3, any method, cis-Tetrahydro-2-oxothieno(3, 4]imidazoline-4-valeric acid, biotin, native protein, natural protein, drIce, drICE, 4-d]imidazol-4-yl]pentanoic acid, Protein, Biotin Ratiopharm, CES2A1, 4-d)imidazoline-4-valeric acid, ABP-280, OF-900, Biodermatin, D-Biotin, Biotin-Ratiopharm, 1H-Thieno(3, ABPL, MFM5, absence, Biotine Roche, Gabunat, FLN2, Fln2, ABPA, content, actin-binding-like protein, culture supernatant, P45, proportionality, hexahydro-2-oxo-, Tripcellim, rate, IL-1 beta-converting enzyme, Interleukin-1 beta-converting enzyme, 4)imidazole-4-valeric acid, FLNC, beta Trypsin, w/w, Protein Gene Products, Interleukin-1 beta convertase, Gene Proteins, Trypure, sodium 2-oxopropanoate, concentration, structure, Drice, 3.4.22.36, quotient, p45, cis-(+)-Tetrahydro-2-oxothieno[3, D-(+)-biotin, Biokur, ABP-LFLN-C, multicellular organismal catabolic process, single-organism catabolic process, Striated Muscles, PRKBA, Akt/PKB, CG5373, dP60, Vps34, ATVPS34, determination, DAKT1/PKB, postnatal development, Myocyte, primitive node, PI3K, Dp110, BcDNA:LD15217, growth and development, protein, AKT1, VPS34, 11621, AKT signalling cascade, phosphorylation, binding site, PI-3 kinase, p110-alpha, protein polypeptide chains, PHOSPHATIDYLINOSITOL 3-KINASE, DmelCG2699, F8A5.4, dorsal marginal zone, node, Filamin-2, ABP280A, Kinase, phosphatidylinositol 3-kinase activity, protein aggregate, Muscle Cell, Dp60, Pi3K92D, CG4141, PI3K_59F, catabolism, catalyst activity, 1110055E19Rik, Pi3k, PI3-kinase activity, proteins, AKT signaling cascade, Striated, PI[[3]]K, PI3Kgamma, PKB|Akt, Fast, RacPK, PKB signaling cascade, MCAP, PKB signalling cascade, p55alpha, Muscles, Mature Muscle Cell, Hensen node, signaling process, PI3K 68D, Hensen's node, Pi3K, PI3k, protein kinase B signal transduction, MCMTC, embryo organizer, FAST, ATP - 1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, single organism signaling, PKB alpha, dVps34, gamma-filamin, PI3K68D, AKT signal transduction, dakt, P110DELTA, phosphatidylinositol 3-kinase signaling/protein kinase B signal transduction, PtdIns-3-kinase activity, rea, p50alpha, FASTK, RAC-PK-alpha, l(3)89Bq, CG2699, DmelCG5373, vacuolar protein sorting 34, dPIK, 1-phosphatidylinositol 3-kinase activity, AKT1 kinase, PKB/Akt, PKB/AKT, AKT/PKB, protein kinase B alpha, C530050K14, dAkt, dAKT, type-1 PI3K, p-Akt, protein kinase B signaling, Henson's node, DAkt, region, l(3)04226, CG11621, DPKB, MFM5, breakdown, positional polypeptide feature, pAkt, p85alpha, growth pattern, FLN2, Fln2, non-developmental growth, actin-binding-like protein, proto-oncogene c-Akt, AI843021, PI3K 68_D, Mature, dAKT/dPKB, PKB/dAKT, FLNC, Filip, DmelCG4141, F8A5_4, protein kinase B, APDS, p110D, Dakt, PI3K/Akt signal transduction, PI3KBETA, dPKB, DMZ, p120-PI3K, 5730485H21Rik, Protein Kinase, droPIK57, RAC-ALPHA, DRAC-PK85, skeletal muscle system., DmelCG4006, biological signaling, PI3K/PKB signal transduction, Myocytes, region or site annotation, PI3K92E, number, STK10, Pi3K_59F, Gene, PKB signal transduction, type I phosphatidylinositol kinase activity, PKB signaling, protein-containing complex, skeletal muscle, presence, phosphatidylinositol 3-kinase, Dpkb, PIK3, dVps34/PI3K59F, Pi3Kp60, polypeptide chain, p60, FILIP, PI3K-dp110, Gene Products, dPI3K, PHOSPATIDYLINOSITOL 3-KINASE, anon-92Ed, FLNc, Dp110/PI3K, PI3K-68D/E, class I, shield, Gamma-filamin, positional, ABP-280-like protein, nodus primitivus, ligand, Spemann's organizer, PI3'K, Pi3Kp110, Muscle Cells, ATP-protein phosphotransferase, P110BETA, PI3K-68D, PI3K21B, Striated Muscle, AKT, ATP:Fas-activated serine/threonine protein phosphotransferase activity, Akt, DRAC-PK, site, MCM, organizer, MPD4, Cpk, striated muscle, dP110, protein kinase B signalling cascade, AKT signaling, filamin-2, AA414921, degradation, akt, polypeptide motif, p120, class II, protein complex, type III phosphoinositide 3-kinase activity, Proteins, Phosphorylations, p110, Actin-binding-like protein, Vps34p, DAkt1, DAKT1, function, embryonic organizer, motif, PI(3)K, cpk, PI3K-PKB/Akt pathway, PI3K-92E/Dp110, development, Mature Muscle Cells, PIK3C1, somatic muscle, count in organism, ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, PKB, CWS6, native protein, CWS5, vps34, natural protein, Protein, chemical analysis, PKB-ALPHA, Spemann Mangold organizer, dp110, D-Akt, CG4006, phosphorylation enrichment, PI3CG, protein kinase B signaling cascade, ABP-280, PI-3-K, Dakt1, serine-threonine kinase Akt, ABPL, PI3K-59F, PI3K-Dp110, akt1, dAkt1, ABPA, Kinases, INSDC_feature:misc_binding, DRAC-PK66, postnatal growth, class III, DmelCG11621, p110gamma, signalling, Protein Gene Products, Gene Proteins, dAkt/PKB, dakt1, CLOVE, binding_or_interaction_site, signalling process, DmVps34, IMD14, PI3K_68D, PI3K-92D, PKBalpha, embryonic shield, Dmp110, dAKT1, RAC, Rac, assay, quantitative, growth, ABP-L, thymoma viral proto-oncogene, skeletal muscle system, presence or absence in organismFLN-C, multicellular organismal catabolic process, single-organism catabolic process, Gamma-filamin, gamma-filamin, ABPL, positional, MFM5, ABP-280-like protein, breakdown, filamin-2, positional polypeptide feature, degradation, FLN2, Experiment., region or site annotation, polypeptide motif, Fln2, catabolism, ABPA, INSDC_feature:misc_binding, actin-binding-like protein, 1110055E19Rik, Phosphorylations, Actin-binding-like protein, AI843021, motif, Filip, FLNC, phosphorylation, binding site, binding_or_interaction_site, FILIP, site, phosphorylation enrichment, Filamin-2, ABP280A, MPD4, 5730485H21Rik, FLNc, region, ABP-L, ABP-280liquid chromatography tandem mass spectroscopy, FLN-C, Bru, IPP2A2, para Tyrosine, E 920, Raw, ion, 1.5.5.1, L-Threonine, protein, L-cysteine, Electron-transferring-flavoprotein dehydrogenase, phosphorylation, 2-Amino-3-hydroxypropionic acid, 5730420M11Rik, peptide, Polypeptides, protein polypeptide chains, L-Isomer Methionine, Methionine, Drug Tolerance, peptido, ClvPrd, Filamin-2, ABP280A, protein aggregate, WMS, Threonin, Svc, SET, C, LCMSMS, peptides, ETF-QO, TAF-I, M, phosphatase 2A inhibitor I2PP2A, 3-(p-Hydroxyphenyl)alanine, 2-amino-3-(4-hydroxyphenyl)propanoic acid, 1110055E19Rik, proteins, Y, DmelCG4299, IGAAD, set, 2-amino-3-hydroxypropanoic acid, DmelCG10574, Tyr, ppm, house mouse, Half Cystine, GPHYSD2, Racemethionine, drug tolerance, SGS, LC-MS2, phapii, gamma-filamin, Zinc Cysteinate, immune system tolerance, acetylation, LC-MS/MS, mouse, 2-Amino-4-(methylthio)butyric acid, 2-Amino-3-(p-hydroxyphenyl)propionic acid, StF-IT-1, para-Tyrosine, Cys, mice C57BL/6xCBA/CaJ hybrid, Tyrosine, ACMICD, 3-Hydroxyalanine, Ionen, Immunologic Tolerance, Methionin, methionine, Hmet, (2R)-2-amino-3-sulfanylpropanoic acid, tyrosine, parent ion, MFM5, Serine, HLA-DR-associated protein II, DI-2, FLN2, Fln2, mice, I-2Dm, actin-binding-like protein, L-isomer, L-Cysteine, CG4299, MASS, inhibitor of granzyme A-activated DNase, FLNC, beta Trypsin, L-Cystein, L isomer, I-2PP1, TAF-IBETA, Self Tolerance, precursor ion, Tolerance, liquid chromatography-tandem mass spectroscopy, L Serine, TAF-Ibeta, Mouse, L-Tyrosine, Polypeptide, L-Serine, i2pp2a, Liquimeth, Proteomes, Cysteine Hydrochloride, 2-amino-4-(methylsulfanyl)butanoic acid, L-Zystein, L Isomer, FBN, precursor, protein-containing complex, PHAPII, LC-MS-MS, template-activating factor I, polypeptide chain, ECTOL1, LC-MSMS, L-Methionine, Del(8)44H, Half-Cystine, FLNc, Pedameth, Gamma-filamin, ABP-280-like protein, L Cysteine, iones, E-920, ipp2a2, beta-Trypsin, Acetylations, 2pp2a, L Threonine, ions, CG10574, OCTD, 2PP2A, 2-amino-4-(methylthio)butanoic acid, 10^[-6], CYSTEINE, L Tyrosine, taf-ibeta, dSET, dSet, FREE CYSTEINE, peptidos, Tyrosin, MPD4, (2R)-2-amino-3-mercaptopropanoic acid, metionina, serine, filamin-2, DL-Methionine, protein complex, igaad, Phosphorylations, total expressed protein, ETF dehydrogenase, Actin-binding-like protein, L-Isomer, Peptide, Mus muscaris, polypeptide, Serin, LC/MS/MS, native protein, Immune Tolerance, natural protein, I-2PP2A, Mus, tirosina, Dm I-2, Protein, I2PP2A, MFS1, phosphorylation enrichment, Data Base, ABP-280, Met, WMS2, ABPL, Col4a-1, Protein., ABPA, Tripcellim, ETF-ubiquinone oxidoreductase, L-2-Amino-3-mercaptopropionic acid, (R)-2-amino-3-mercaptopropanoic acid, Trypure, dSET/TAF-Ibeta, 2610030F17Rik, Ion, SSKS, alpha-amino-gamma-methylmercaptobutyric acid, liquid chromatography tandem mass spectrometry, E920, Peptid, threonine, variable, AA407739, ABP-L, Immunological ToleranceFLN-C, multicellular organismal catabolic process, single-organism catabolic process, gamma-filamin, ABPL, positional, ABP-280-like protein, breakdown, filamin-2, positional polypeptide feature, degradation, FLN2, region or site annotation, polypeptide motif, catabolism, INSDC_feature:misc_binding, Phosphorylations, AI843021, motif, Filip, FLNC, phosphorylation, binding site, binding_or_interaction_site, FILIP, site, phosphorylation enrichment, actin-binding-like protein., 5730485H21Rik, FLNc, region, ABP-LfalsePhosphoproteomics Identifies Dual-Site Phosphorylation in an Extended Basophilic Motif Regulating FILIP1-mediated Degradation of filamin-C FLNc d18-21 BioID experimentFLNc, the muscle-specific isoform of the filamin family, is a multi-adaptor protein, comprising 1 amino-terminal actin-binding (ABD) domain followed by 24 immunoglobulin-like (Ig) domains. While FLNc can form homodimers via the last Ig-like domain and thus function as an actin-crosslinker like the other filamins, it features a unique insertion of 82 amino acids (aa) in domain 20. This insert was not only shown to mediate the interaction to several FLNc binding partners, but also to contain an Akt-mediated phosphorylation site at S2234 of mouse FLNc (mFLNc). To reveal novel proteins in the nano-environment of FLNc within myotubes under mild electrical pulse stimulation conditions, we applied a quantitative BioID appraoch.2021-07-122021-07-12PXD009159646099180498511145422492156843544071284218327042363336351198343006636362758385696363121766611143015557114526314526236493461312695736664923916193641364510665688951136364762723661112329442229555832681304232312258636543460792519365910632143205971293865581912502321195774818665867225680761755437359253668586274081691804542787751201089367414795641003923694204943344597458641NCBITaxon:776436715675936438677160540483131528269304562716391624292042779443696121810032962922113763948840479NCBITaxon:2157131527310510671250278205968720165163684071736309698936148364320429294101061320491381693320464535111103217459216129288671288676410069456941016763410210761277351131658213584301417298174633675412873567568403013506218130194111929439367110411364459139498432025301270320221498751104759412047886134749515372110971652998279051355513980317585439801260866398081375755518320196447111415542193255521138343911931819813981810269701069955529NCBITaxon:47519833547641465477982510216915727398238976484074186287054824153NCBITaxon:50557984467525100036454802103597413708115466547898353221599838106771034819852348780422531488414222863226634794615718712593991599191572952929211752719860148840633424951869123423523815233598589859886559746499870392021110723635272159874287434182350241451139692931902593907190174636012374746361722625360013197221191585310588596989511051989972379897263419610264266722758858156899628737562690724420237172019998570724074767927459615333554319311671833554184005136861505968666211961748666134751536329NCBITaxon:176328628711033348837670580271848186536859598607575527472036105135294296110098402321000734882412336363071047005187878945556449447NCBITaxon:13356262664955419591399811901781681478281055524NCBITaxon:960613585846900813668481979153519307285930085264114090051184995262254121337591390143811139442370416744312044143708351581990119519532590841370137023943222774862615835583715371633376057267371137121906503714759172073127262981274575727090309031637910372814243371421044792491108239412NCBITaxon:961565885814780269266100658137352750454135663816475203750644223669502129349763224555253651997649908143899237453747264732376026473128870559553614169152527403271602938511497864794363755293824794372251177529916738721750919384413765327159100226423444234546700128768959119846704146479293652803428038280351080348179392112621229502711311803738681911265269971028621076015973611432425071140981087135547743658142450722953379698204232124223321803369822347081645733185889716546759928777020141252955081148174488864374526499972830357183085507176299926837041515628876513885639245611520865955516551814321371597495350261432138587817076831949634016142800531646862751104660381518454744850097476974771180512401748418556015554792042742014887110532532972610059412890931643512116049900138943828354232423310829341220585991311227317018799154291312678181871049316407299467303337330485264638301130641399253064236121162065261610337286129940115357652611114716133865431201725323712552329935316317NCBITaxon:110849938410632678728623219334862086211607913922276811961255228149539321322323347741218275329175731299488618140843410335199631392489139248811533811533933043937556992862655993391120948862415718399758644423748643533244426066533241343909768212717863719798568639998399868655352914865410429863573582576328840198649823801119110286889995866522034163213811938062563188657130344386581144889198466645463NCBITaxon:11072NCBITaxon:110711428006288353743751165971459431165988543311167810778543144080439334NCBITaxon:11082693977381382189931824431935128387145953116885311213278695berry869410033358697244366470150111428692150475149910548420471472235402512084701464974449743117008930621864419306110763233080566039601621797482585394396513212790568813377512981766017112147861050236694657349632051124501897441739412450128853183889198679488531911137910371647351660152943631631198573896939343237956026087121346334323223544314275241433611334565560291699636436802790105603140818838185733703401881274414437552196969713985584081724055986091838271274423408170275981274420127442623545513656533821176275943282474801199150956481882291690255120455148167238473848190695148498101510693731263753405203860186497405224052141856451804NCBITaxon:813478009152275484793870333849387191722617561094616143512032581205505235259447320549386411257226512165125388037668612067811220223074322011867057387334869121938791868023980071868012154514683514081861508471206777691838851608611388869152167786721279010087043512317196928123929486771331548454226126219813411229265716329576938124712461485451328388348392949113903631204162665890771514880036942694169436945187901015080817053942065421055687317447127015329242814111946691169601454586953201019812646905505371408157697392830312383911283128212802811010832825253531522811235315349825741948153623142410145481NCBInr 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