Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R2_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R4_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R4_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R2_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R2_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R3_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R1_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R2_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R2_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R1_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R3_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R4_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R3_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R3_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R1_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R1_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R2_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R3_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R1_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R4_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R2_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R4_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R2_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R1_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R4_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R3_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R4_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R2_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R4_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R3_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R2_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R1_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R1_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R3_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R4_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R3_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R1_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R4_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R3_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R3_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R1_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R1_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R2_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R3_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R3_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R4_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R2_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R3_F3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R2_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R1_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R1_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R4_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R4_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R2_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R3_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R3_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R1_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R4_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Control_R1_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R2_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R4_F4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R2_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R1_F5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R4_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R4_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R3_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R2_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R1_F2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_Olap-H2O2_R4_F1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R2_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R3_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/QE6_IAH-SCL_PHOS_H2O2_R1_F6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/HUMAN.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009931/MaxQuant_Output.zipprimaryOK200002310michael.lund.nielsen@cpr.ku.dkIvo HendriksAffinity purification coupled with mass spectrometry proteomicsMass SpectrometryNot availableAdp-ribosylationPhosphorylationAdphttp://www.ebi.ac.uk/pride/archive/projects/PXD009931Permanent Cell Line CellCell CultureHeLa cells were either left untreated or treated for 10 min with 1 mM H2O2, and with or without one hour pre-treatment with the PARP inhibitor Olaparib (10 μM) in case of H2O2 treatment. All experiments were carried out in biological cell culture quadruplicate (n=4). For all experiments, cells were harvested in ice-cold PBS, lysed in 6 M guanidine buffer, reduced and alkylated, and subsequently digested with Lys-C and trypsin. Phosphorylated peptides were enriched using TiO2 beads. Subsequently, samples were high-pH fractionated on StageTip. All samples were analyzed on 15-cm long analytical columns, with an internal diameter of 75 μm, and packed in-house using ReproSil-Pur 120 C18-AQ 1.9 µm beads (Dr. Maisch). Reversed-phase liquid chromatography was performed using an EASY-nLC 1200 system (Thermo). The analytical column was heated to 40 °C, and elution of peptides from the column was achieved by application of gradients with Buffer A (0.1% formic acid) and increasing amounts of Buffer B (80% ACN in 0.1% formic acid). The gradient ranged from 5% buffer B to 50% buffer B over 82 minutes, followed by a washing block of 18 minutes. Electrospray ionization (ESI) was achieved using a Nanospray Flex Ion Source (Thermo), with the ions analyzed using a Q Exactive HF-X mass spectrometer (Thermo). Spray voltage was set to 2 kV, capillary temperature to 275 °C, and S-Lens RF level to 40%. Full scans were performed at a resolution of 60,000, with a scan range of 300 to 1,500 m/z, a maximum injection time of 60 ms, and an automatic gain control (AGC) target of 3e6 charges. Precursors were isolated with a width of 1.3 m/z, with an AGC target of 2e5 charges, and precursor fragmentation was accomplished using higher energy collision dissociation (HCD). Only precursors with charge state 2-5 were considered. Selected precursors were excluded from repeated sequencing by setting a dynamic exclusion of 90 seconds. MS/MS spectra were acquired in the Orbitrap, and settings included a loop count of 9, a maximum injection time of 90 ms and a resolution of 45,000.PrideMS1 intensity based label-free quantification methodphosphorylated residueFor the data search, all RAW files were analyzed using the freely available MaxQuant software (version 1.5.3.30). Default MaxQuant settings were used, with exceptions outlined below. For generation of the theoretical spectral library, the HUMAN.fasta database was extracted from UniProt on the 23rd of January, 2018, and in silico digested with trypsin, allowing up to 8 missed cleavages and a maximum peptide mass of 7,000 Da. Protein N-terminal acetylation, methionine oxidation, and phosphorylation of serine, threonine, and tyrosine residues were included as potential variable modifications. A maximum allowance of 4 variable modifications per peptide was used. Label-free quantification (LFQ) was enabled. Second peptide search was enabled (default), and matching between runs was enabled with a match time window of 1 minute and an alignment time window of 20 minutes. Modified peptides were filtered to have a delta score of at least 20. Data was additionally filtered by posterior error probability to achieve a false discovery rate of <1% (default), at the peptide-spectrum match, the protein assignment, and the site-specific levels.ProteomicsMichael Lund NielsenQ Exactive HF-XProteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, DenmarkPARTIALHomo Sapiens (human)30157440 Larsen SC, Hendriks IA, Lyon D, Jensen LJ, Nielsen ML. Systems-wide Analysis of Serine ADP-Ribosylation Reveals Widespread Occurrence and Site-Specific Overlap with Phosphorylation. Cell Rep. 2018 24(9):2493-2505.e4 10.1016/j.celrep.2018.07.083ivo.a.hendriks@gmail.comBiologicalProteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkDenmarkADP-ribosylation (ADPr) is a reversible posttranslational modification involved in a range of cellular processes. Here, we report system-wide identification of serine ADPr in human cells upon oxidative stress. High-resolution mass spectrometry and unrestricted data processing confirm that serine residues are the major target of ADPr in HeLa cells. Proteome-wide analysis identifies 3,090 serine ADPr sites, with 97% of acceptor sites modulating more than 2-fold upon oxidative stress, while treatment with the poly (ADP-ribose) polymerase (PARP) inhibitor olaparib abrogates this induction. Serine ADPr predominantly targets nuclear proteins, while structural-predictive analyses reveal that serine ADPr preferentially targets disordered protein regions. The identified ADP-ribosylated serines significantly overlap with known phosphorylated serines, and large-scale phosphoproteomics analysis provides evidence for site-specific crosstalk between serine ADPr and phosphorylation. Collectively, we demonstrate that serine ADPr is a widespread modification and a major nuclear signaling response to oxidative stress, with a regulatory scope comparable to other extensive posttranslational modifications.Systems-wide Analysis of Serine ADP-Ribosylation Reveals Widespread Occurrence and Site-Specific Overlap with Phosphorylation.Larsen Sara C SC, Hendriks Ivo A IA, Lyon David D, Jensen Lars J LJ, Nielsen Michael L MLsodium salt, CPD photolyase activity, DmelCG40411, ammonium formate, 13C-labeled, IL1BC, cadmium salt, PhrB photolyase activity, ion, H2O2, CASP-1, Longterm., Reversed-Phase Chromatography, chronic obstructive pulmonary disease (COPD), magnesium formate, dihydrogen dioxide, zinc salt, Long Term, 5730420M11Rik, dmTAF[[II]]230, lenses, Polypeptides, T22F8_160, cobalt(II) formate dihydrate, ATGPR7, B1, Chronic irreversible airway obstruction, GRP8, 3, GRP7, NUP96, outer pigmented layer of retina, Il1bc, epithelium, ADPRT, CG17696, SET, C79325, Adprp, TFIID TAF250, sarcoma of breast, cel, ADPRT1, AI893648, phosphatase 2A inhibitor I2PP2A, ocular lens, CAFL - Chronic airflow limitation, IL-1BC, SUPPRESSOR OF AUXIN RESISTANCE 3, AI047805, DmelCG4299, set, pigment epithelium of retina, decreased, h, column, disease (COPD), ACN, D1, Acn, Chromatography, pigmented retina epithelium, z, nickel salt, dTAF[[II]]230, chronic obstructive lung disease [Ambiguous], Caspase-1 subunit p10, BZRP, cobalt (+2) salt, Trypsin/K, TAF200, Longterm Effect, Guanidine Monohydroiodine, breast sarcoma, not genetically inherited, DmelCG7788, PBS, PBR, CHRONIC OBSTRUCTIVE AIRWAY DIS, sodium (4:1:1) salt, count, Dm1, magnesium salt, dipyrimidine photolyase (photosensitive), Airflow Obstruction, GLYCINE-RICH PROTEIN 8, Chronic Obstructive Airways Disease, Chronic obstructive lung disease, HLA-DR-associated protein II, DI-2, lens, I-2Dm, formate, Guanidine Phosphate, cromium (+3), inhibitor of granzyme A-activated DNase, beta Trypsin, I-2PP1, Reversed Phase Chromatography, Painful bladder syndrome, TAF-IBETA, F2G1.4, Taf250, OBSTRUCTIVE PULMONARY DISEASE (COPD), ADPRT 1, Cells, Drice, ammonium (4:1) salt, HKE5, TAF-Ibeta, 5830444G22Rik, DRICE, lead (+2) salt, TAF230, bis(hydridooxygen)(O--O), BPBS, HOOH, breadth, nickel formate dihydrate, lead formate, sarcoma of the breast, Effects, LD21673.3prime, DrIce, DrICE, aluminum salt, CAO - Chronic airflow obstruction, Lens <eudicots>, CHRONIC OBSTRUCTIVE PULM DIS, Reverse-Phase Liquid Chromatography, AZD221, precursor, Chronic obstructive pulmonary disease NOS, thomson, body system, CG7826, deoxyribodipyrimidine photolyase activity, and RNA binding 1, lens crystallina, stratum pigmentosum retinae, mass-to-charge ratio, Chronic obstructive pulmonary disease finding, period, AZD-2281, PULMONARY DISEASE (COPD), Guanidium, CG7835, CG42273, system, C80510, AZD2281, Monohydroiodine, anatomical systems, dTAF[[II]]250, Acinus, thallium (+1) salt, Longterm, cell, PCE-2, beta-Trypsin, rubidium salt, F23A5.3, 2pp2a, pk18, Long-Term, adprt1, methanoic acid, ESI, ions, MBR, CG10574, acinusL, Injectable, dTAF250, Monohydrobromide, Guanidinium Chloride, 2PP2A, Caspase-1 subunit p20, ATGRP7, acinusS, ATGRP8, Reversed-Phase Liquid, NEC, dSET, dSet, PTBR, Chronic, peptidos, parp-1, PTHB1, inhibitors, Guanidine Monohydrate, Guanidine Nitrate, lens capsule, chronic obstructive airways disease NOS, caspase 3, COAD, BG:DS00004.13, potassium formate, CE-2, copper (+2) salt, COLD - Chronic obstructive lung disease, buffer, Cell, chronic, mKIAA0670, dTAF230, polypeptide, Temperatures, I-2PP2A, C18, cupric formate, Dm I-2, Long Term Effects, TAF[[II]]250/230, Nitrate, (COPD), chronic airway obstruction, chronic obstructive, aluminum formate, CAL - Chronic airflow limitation, AU020952, Taf[[II]]250, Guanidine Monohydrobromide, RPE, Guanidine, lithium formate, Guanidine Sulfate, underdeveloped, GR-RBP7, GR-RBP8, Chloride, IL-1 beta-converting enzyme, Interleukin-1 beta-converting enzyme, mDRC, retinal pigment epithelium, Longterm Effects, ME-IV, p. pigmentosa retinae, padprt-1, FBCG2, PARP-1, D.PARP, chronic obstructive lung disease (disorder), deoxyribonucleate pyrimidine dimer lyase (photosensitive), Sulfate, chronic obstructive pulmonary disease, IPP2A2, T22F8.160, and rna binding 2, Chronic Airflow Obstructions, AZD 2281, zinc formate, Oxydol, CG7788, crice, Monohydrate, HYDROGEN PEROXIDE, Guanidine Sulfate (2:1), lead salt, pigmented epithelium, Adprt1, peptide, ammonium tetraformate, peptido, BANF, chronic obstructive airways disease NOS (disorder), Min, Chronic Obstructive Pulmonary Disease (COPD), present in fewer numbers in organism, Injectables, Effect, DmelCG42273, perhydrol, COLD (chronic obstructive lung disease), peptides, reference sample, ARTD1, NEC in ICD9CM_2006, TAF-I, long, 2610036I19Rik, 2610510L13Rik, pigmented retina, hypoplasia, min, crystalline lens, mAPC, IBP, Phosphate, copper, SCAN, DNA cyclobutane dipyrimidine photolyase activity, Chronic airflow limitation, IGAAD, fSAP152, Reverse Phase, lithium salt, DmelCG10574, ICE, GLYCINE RICH PROTEIN 7, Guanidine Sulfate (1:1), blood capillary, Bladder pain syndrome, glycine-rich RNA-binding protein 8, CHRONIC OBSTRUCTIVE PULMONARY DISEASE, ammonium (2:1) salt, Long-Term Effects, Lynparza, CHRONIC, phapii, PRE, obstructive lung disease, Monohydrochloride, ice, iCE, drice, Reverse Phase Chromatography, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, Dmel_CG7826, pADPRT-1, StF-IT-1, Th, pigmented retinal epithelium, TAFII-250, TAF250/230, Airflow Obstructions, DFNA13, pulmonary disease (COPD), PARP1, TAFII250, batch, HCD, retinal pigment, Ionen, drIce, drICE, low temperature, CHRONIC OBSTRUCTIVE, chronic obstructive pulmonary disease and allied conditions, Chronic airway disease, NOS, nickel (+2) salt, retinal pigment layer, Dmel_CG7835, COPD NOS, Mnb, MNB, CHRONIC OBSTRUCTIVE LUNG DIS, parent ion, Chronic airway obstruction, Hydrogen peroxide, Reversed-Phase, ammonium salt, Painful Bladder Syndrome, CHARGES, dioxidane, cold, retinal pigmented epithelium, P45, cobaltous formate, Guanidine Monohydrochloride, CG4299, AW124434, CG17603, TAF[[II]], DFNB53, organ system, CG17718, Chronic Airflow Obstruction, nanospray, phr A photolyase activity, DNA-photoreactivating enzyme, precursor ion, SR3-5, COPD - Chronic obstructive pulmonary disease, copper salt, chronic obstructive airways disease, p45, Polypeptide, obstructive pulmonary disease (COPD), i2pp2a, COLD, chronic obstructive lung disease, time, Lens <bivalves>, Bladder Pain Syndrome, d230, DISEASE (COPD), cesium salt, dTAFII250, BEST:LD21673, circadian rhythm, ACINUS, photoreactivating enzyme activity, EfW1, PHAPII, Buffer, formic acid, Cold, potassium salt, CG40411, Pulmonary Disease, reduced, template-activating factor I, dmTAF1, Taf230, subnumerary, Injection, 14C-labeled, stratum pigmentosa retinae, camera-type eye lens, tiny, TAF250, Guanidium Chloride, Taf200, Cold Temperatures, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, PRECOCIOUS, iones, HeLa, ipp2a2, inhibiteur, Taf1p, dihydrogen peroxide, decreased number, calcium formate, Chronic Obstructive, inhibidor, taf-ibeta, [OH(OH)], cromium (+3) salt, Long-Term Effect, TAF, eye lens, Guanidine Hydrochloride, F23A5_3, sodium formate, Controlled, COPD, small, DYRK1, COAD - Chronic obstructive airways disease, Controlling, TAF[[II]]250, nickel formate, DBI, not elsewhere classified, dihydrogen(peroxide), igaad, strontium formate, inhibitor, l(3)84Ab, strontium salt, PULM DIS CHRONIC OBSTRUCTIVE, Guanidinium, Peptide, Chronic obstructive pulmonary disease finding (finding), stratum pigmentosum (retina), MODIFIER OF SNC1, capillary vessel, hydrogen peroxide, HeLa Cell, PKBS, sPARP-1, p230, STL3, deoxyribonucleic photolyase activity, I2PP2A, Hydrochloride, Reversed-Phase Liquid Chromatography, Guanidine Sulfite (1:1), TFIID, Dyrk1, CES2A1, GLYCINE-RICH RNA-BINDING PROTEIN 7, connected anatomical system, Reverse-Phase Chromatography, chronic obstructive airway disease, Temperature, TAF[[II]]230, PARP, photolyase activity, Dops, CC1, Tripcellim, TAF[II]250, parp, CG17685, chromic formate, Interleukin-1 beta convertase, Trypure, dSET/TAF-Ibeta, 2610030F17Rik, DmelCG17603, ppol, Ion, 3.4.22.36, calcium salt, cold (chronic obstructive lung disease), Peptid, dPARP, Chronic Obstructive Lung Disease, Dm.pARTa, PPOL, SCH, AA407739, TAF1serine, ADPRibosylation, MARylation, wide/broad, determination, region or site annotation, ADP, frequency, Mono(ADP-Ribosyl)ation, Phosphorylations, broad, Ribosylation, temporal, binding site, 2-Amino-3-hydroxypropionic acid, Ribosylations, Serin, (ADP-Ribosyl)ation, Mono, 3-Hydroxyalanine, MARylations, chemical analysis, Mono ADPRibosylations, Mono ADP Ribosylation, MonoADPRibosylations, overlap, outbreaks, Mono(ADPRibosyl)ation, (ADPRibosyl)ation, region, Mono ADPRibosylation, positional, Serine, positional polypeptide feature, occurrence, INSDC_feature:misc_binding, ADP-Ribosylations, prevalence, ADPRibosylations, ADP Ribosylation, surveillance, morbidity, endemics, Mono-ADP-Ribosylations, Mono-ADPRibosylations, MonoADPRibosylation, wide, Mono-ADP-Ribosylation, ADP Ribosylations, 2-amino-3-hydroxypropanoic acid, binding_or_interaction_site, site, L Serine, epidemics, assay, phosphorylation., L-Serine, incidence, Mono-ADPRibosylationRps17, Bru, para Tyrosine, delta, Raw, RPS17, L-Threonine, protein, phosphorylation, Long Term, UCRBP, 2-Amino-3-hydroxypropionic acid, peptide, M(3)67, Polypeptides, YIN-YANG-1, protein polypeptide chains, L-Isomer Methionine, M(3L)i, Methionine, ClvPrd, peptido, posterior, 0926/11, Software Engineering, protein aggregate, Effect, Computer Program, WMS, Threonin, Svc, D, DELTA, F, peptides, M, C1, Open, 2-amino-3-(4-hydroxyphenyl)propanoic acid, 3-(p-Hydroxyphenyl)alanine, l(3)67BDo, Computer Programs and Programming, min, proteins, Y, free, DELTA1, 2-amino-3-hydroxypropanoic acid, Tyr, delta D1, s, D9, Delta, GPHYSD2, Racemethionine, M(3)RpS17, CG3619, Long-Term Effects, posterior end of organism, SGS, ratio, DL, 1053/14, acetylation, 2-Amino-4-(methylthio)butyric acid, 2-Amino-3-(p-hydroxyphenyl)propionic acid, Longterm Effect, M(3)67C, para-Tyrosine, Source Softwares, Software Tools, Programs, ACMICD, Tyrosine, Program, Computer Applications, DL1, 3-Hydroxyalanine, M(3)i(55), NF-E1, Computer Applications Software, Computer Applications Softwares, Methionin, methionine, Softwares, Hmet, tyrosine, region, rpS17, Software Applications, Serine, positional polypeptide feature, Source Software, DmelCG3619, Rp S17, L-isomer, proportionality, rate, MASS, 1304/03, beta Trypsin, human, CT12133, L isomer, Applications, 2018, L Serine, L-Tyrosine, Polypeptide, l(3)92Ab, L-Serine, Liquimeth, time, Computer Software Applications, l(3)j8C3, human being, 2-amino-4-(methylsulfanyl)butanoic acid, Effects, False, region or site annotation, anon-WO0118547.269, L Isomer, FBN, Computer, protein-containing complex, period, M(3)S33, M(3)i, polypeptide chain, ECTOL1, L-Methionine, Del(8)44H, Pedameth, Application, Open Source Softwares, proportion, positional, Longterm, 1119/09, Software Application, INO80S, beta-Trypsin, Open Source Software, Acetylations, Minute, L Threonine, Long-Term, l(3)dtOA4, man, Probabilities, Computer Software Application, OCTD, 2-amino-4-(methylthio)butanoic acid, Tools, DmelCG3922, L Tyrosine, LMPY, M(3)i[55], dmDelta, site, peptidos, Long-Term Effect, Tyrosin, metionina, Applications Software, serine, Open Source, data, Computer Software, l(3)05151, DL-Methionine, protein complex, 1440/11, Phosphorylations, L-Isomer, Peptide, Tool, polypeptide, Serin, Software Tool, native protein, natural protein, tirosina, Protein, Long Term Effects, 0495/20, MFS1, BcDNA:RE44119, Software, Library, Data Base, WMS2, Met, Col4a-1, INSDC_feature:misc_binding, 1423/11, M(3)q, Engineering, Tripcellim, E(ls)2, anon-EST:Posey48, Longterm Effects, CG3922, Computer Programs, Trypure, Applications Softwares, 1485/04, binding_or_interaction_site, SSKS, alpha-amino-gamma-methylmercaptobutyric acid, M(3L)i[55], quotient, Peptid, threonine, variable, binding site.ADPRibosylation, DNA Oxidative, DmelCG40411, scale tissue, determination, Ass-1, AZD 2281, Mono(ADP-Ribosyl)ation, Mbp1, Nitrative Stress, Damage, phosphorylation, binding site, 2-Amino-3-hydroxypropionic acid, dmTAF[[II]]230, Adprt1, Adprt2, %, Oxidative DNA, Oxidative, MARylations, AA408052, Nucleolar Protein, Nitro-Oxidative Stress, NAD(+) ADP-ribosyltransferase 2, NAD(+) ADP-ribosyltransferase 1, fold, Nitro-Oxidative Stresses, Analysis, Oxidative Injury, ADPRTL2, myd, ADPRTL3, DNA Damage, ADPRT, CG17696, Mass Spectrum Analysis, A, Oxidative Injuries, Nuclear Protein, Adprp, TFIID TAF250, Analyses, ARTD1, cel, ARTD2, ADPRT1, Adprt, AI893648, ADPRT2, plant peltate hair, Oxidative Cleavage, Mbp-1, Oxidative DNA Damages, Anti-oxidative, ASS, Oxidative Stress Injuries, Oxidative Stresses, 2-amino-3-hydroxypropanoic acid, Adprtl2, ADPRT-2, Mono-ADPRibosylation, accepteur, Lynparza, F16D14.16, dTAF[[II]]230, MARylation, wide/broad, gyltl1b-b, ADP, TAF200, pADPRT-1, pADPRT-2, TAFII-250, TAF250/230, Spectrum Analysis, F16D14_16, DFNA13, Ribosylations, Spectroscopy, PARP1, (ADP-Ribosyl)ation, TAFII250, 3-Hydroxyalanine, Oxidative and Nitrosative Stress, MDDGA6, mKIAA0609, MonoADPRibosylations, overlap, DNA Oxidative Damages, region, fg, Hydrogen-acceptor, mPARP-2, Serine, gyltl1b, positional polypeptide feature, mdc1d, ADPRibosylations, Spectrometry, expanded, ADP Ribosylation, CG17603, TAF[[II]], DFNB53, human, Nitro-Oxidative, CG17718, Acceptor, MonoADPRibosylation, data analysis, MDC1D, wide, Mono-ADP-Ribosylation, enr, Taf250, enlarged, SR3-5, ADPRT 1, Cells, msPARP, HKE5, L Serine, Oxidative Stress Injury, DNA, 5830444G22Rik, L-Serine, Oxidative Damage., 2.4.2.30, Proteomes, TAF230, Oxidative Damage, big, Oxidative Stress, d230, Akzeptor, human being, region or site annotation, Antioxidative, LD21673.3prime, Aspartl2, peltate hair, Antioxidative Stress, dTAFII250, BEST:LD21673, broad, Spectrum Analyses, AZD221, EfW1, froggy, Gyltl1a, Stresses, AZD-2281, large, Mono, Oxidative Nitrative, CG40411, dmTAF1, Stress Injury, Taf230, Mass, Mono ADPRibosylations, Oxidative Cleavages, Mono ADP Ribosylation, C80510, AZD2281, (ADPRibosyl)ation, Oxidative Damages, Mass Spectroscopy, TAF250, Taf200, positional, Injury, dTAF[[II]]250, cell, MDDGB6, HeLa, inhibiteur, Taf1p, Poly[ADP-ribose] synthase 1, adprt1, Poly[ADP-ribose] synthase 2, man, Oxidized donor, C78626, dTAF250, BPFD#36, ADP Ribosylations, data processing, inhibidor, Anti oxidative Stress, Oxidative DNA Damage, Antioxidative Stresses, great, scale (sensu Metazoa), site, TAF, parp-1, serine, inhibitors, poly(ADP-ribose) polymerase 1, data, TAF[[II]]250, Proteins, Phosphorylations, total expressed protein, l(3)84Ab, inhibitor, BG:DS00004.13, Anti-oxidative Stresses, Ribosylation, Cell, ATPARP1, dTAF230, Serin, Oxidative Nitrative Stress, HeLa Cell, p230, Oxidative Nitrative Stresses, sPARP-1, STL3, Protein, chemical analysis, TAF[[II]]250/230, TFIID, Nuclear, scales, ADP-ribosyltransferase diphtheria toxin-like 1, Mono(ADPRibosyl)ation, Mass Spectrum Analyses, ADP-ribosyltransferase diphtheria toxin-like 2, Taf[[II]]250, Mono ADPRibosylation, Mass Spectrum, TAF[[II]]230, Anti-oxidative Stress, scale, DNA Oxidative Damage, PARP, INSDC_feature:misc_binding, ADP-Ribosylations, TAF[II]250, Nucleolar Proteins, Cleavage, parp, CG17685, Mono-ADP-Ribosylations, Mono-ADPRibosylations, Parp-1, DmelCG17603, padprt-1, binding_or_interaction_site, ppol, PARP-2, FBCG2, PARP-1, Stress, D.PARP, dPARP, assay, Dm.pARTa, PPOL, Nitro Oxidative Stress, Nucleolar, hPARP-2, TAF1projections, ADPRibosylation, DNA Oxidative, ribo-pentose, DmelCG40411, scale tissue, posttranslational modification, DCAF9, determination, Ass-1, AZD 2281, Mono(ADP-Ribosyl)ation, Mbp1, Nitrative Stress, CG5124, protein, Damage, phosphorylation, binding site, 2-Amino-3-hydroxypropionic acid, dmTAF[[II]]230, Adprt1, protein polypeptide chains, neutrophilic leucocyte, Oxidative DNA, Oxidative, MARylations, AA408052, neutrophil leukocyte, Nucleolar Protein, Nitro-Oxidative Stress, fold, Nitro-Oxidative Stresses, Analysis, anon-WO0196371.27, Oxidative Injury, protein aggregate, myd, DNA Damage, ADPRT, CG17696, Mass Spectrum Analysis, A, Oxidative Injuries, posttranslational amino acid modification, Nuclear Protein, Adprp, TFIID TAF250, Analyses, ARTD1, cel, ADPRT1, AI893648, plant peltate hair, Oxidative Cleavage, Mbp-1, proteins, mature neutrocyte, Oxidative DNA Damages, Anti-oxidative, ASS, Oxidative Stress Injuries, Oxidative Stresses, 2-amino-3-hydroxypropanoic acid, ribose, papilla, signaling process, polymorphonuclear leukocyte, polynuclear neutrophilic leucocyte, single organism signaling, Mono-ADPRibosylation, accepteur, Lynparza, dTAF[[II]]230, MARylation, anatomical protrusion, wide/broad, gyltl1b-b, ADP, lamina, TAF200, flanges, pADPRT-1, TAFII-250, TAF250/230, Spectrum Analysis, DFNA13, Ribosylations, Spectroscopy, DmelCG5124, neutrocyte, PARP1, (ADP-Ribosyl)ation, TAFII250, 3-Hydroxyalanine, Oxidative and Nitrosative Stress, adp, MDDGA6, mKIAA0609, shelf, MonoADPRibosylations, overlap, DNA Oxidative Damages, anon-WO0196371.1, anon-WO0196371.3, region, fg, Hydrogen-acceptor, Serine, gyltl1b, PTM, positional polypeptide feature, mature neutrophil leukocyte, shelves, mdc1d, ADPRibosylations, Spectrometry, expanded, ADP Ribosylation, CG17603, projection, TAF[[II]], ridge, DFNB53, human, organ system, Nitro-Oxidative, CG17718, Acceptor, MonoADPRibosylation, data analysis, MDC1D, Mono-ADP-Ribosylation, wide, enr, Taf250, enlarged, spine, Rib, post-translational modification, SR3-5, ADPRT 1, Cells, HKE5, L Serine, Oxidative Stress Injury, DNA, 5830444G22Rik, L-Serine, Proteomes, TAF230, Oxidative Damage, big, polynuclear neutrophilic leukocyte, Oxidative Stress, d230, biological signaling, Akzeptor, human being, region or site annotation, lamellae, Antioxidative, LD21673.3prime, peltate hair, Antioxidative Stress, dTAFII250, Gm695, BEST:LD21673, broad, Spectrum Analyses, AZD221, protein-containing complex, EfW1, process of organ, body system, froggy, Gyltl1a, protrusion, Stresses, lamella, AZD-2281, large, Mono, Oxidative Nitrative, CG40411, polypeptide chain, dmTAF1, Stress Injury, Taf230, Mass, Mono ADPRibosylations, Oxidative Cleavages, Mono ADP Ribosylation, system, C80510, AZD2281, (ADPRibosyl)ation, Oxidative Damages, Mass Spectroscopy, TAF250, Taf200, positional, anatomical systems, Injury, dTAF[[II]]250, cell, MDDGB6, neutrophil leucocyte, HeLa, inhibiteur, Taf1p, ridges, posttranslational protein modification, neutrophilic leukocyte, adprt1, man, Oxidized donor, dTAF250, BPFD#36, ADP Ribosylations, mature neutrophilic leucocyte, data processing, inhibidor, Anti oxidative Stress, Oxidative DNA Damage, Antioxidative Stresses, great, scale (sensu Metazoa), site, TAF, parp-1, laminae, signalling., serine, inhibitors, TAF[[II]]250, mature neutrophil leucocyte, protein complex, anatomical process, Proteins, Phosphorylations, total expressed protein, l(3)84Ab, inhibitor, BG:DS00004.13, Anti-oxidative Stresses, Ribosylation, Cell, dTAF230, Serin, Oxidative Nitrative Stress, HeLa Cell, native protein, natural protein, p230, Oxidative Nitrative Stresses, sPARP-1, chemical analysis, STL3, Protein, TAF[[II]]250/230, TFIID, Nuclear, connected anatomical system, scales, Mono(ADPRibosyl)ation, flange, Mass Spectrum Analyses, organ process, Taf[[II]]250, Mono ADPRibosylation, Mass Spectrum, TAF[[II]]230, Anti-oxidative Stress, scale, DNA Oxidative Damage, PARP, INSDC_feature:misc_binding, ADP-Ribosylations, TAF[II]250, Nucleolar Proteins, mature neutrophilic leukocyte, Cleavage, parp, CG17685, post-translational amino acid modification, Mono-ADP-Ribosylations, Mono-ADPRibosylations, processes, process, DmelCG17603, padprt-1, D Ribose, binding_or_interaction_site, signalling process, ppol, adipose, FBCG2, PARP-1, Stress, D.PARP, PMN, processus, polymorphonuclear leucocyte, D-Ribose, assay, dPARP, Dm.pARTa, poly, PPOL, Nitro Oxidative Stress, Nucleolar, polymorphonuclear neutrophil, TAF1serine, ADPRibosylation, MARylation, wide/broad, determination, region or site annotation, ADP, frequency, Mono(ADP-Ribosyl)ation, Phosphorylations, broad, Ribosylation, phosphorylation, temporal, binding site, 2-Amino-3-hydroxypropionic acid, Ribosylations, Serin, (ADP-Ribosyl)ation, Mono, 3-Hydroxyalanine, MARylations, chemical analysis, Mono ADPRibosylations, Mono ADP Ribosylation, MonoADPRibosylations, overlap, outbreaks, Mono(ADPRibosyl)ation, (ADPRibosyl)ation, region, Mono ADPRibosylation, positional, Serine, positional polypeptide feature, occurrence, INSDC_feature:misc_binding, ADP-Ribosylations, prevalence, ADPRibosylations, ADP Ribosylation, surveillance, morbidity, endemics, Mono-ADP-Ribosylations, Mono-ADPRibosylations, MonoADPRibosylation, wide, Mono-ADP-Ribosylation, ADP Ribosylations, 2-amino-3-hydroxypropanoic acid, binding_or_interaction_site, data., site, L Serine, epidemics, assay, L-Serine, incidence, Mono-ADPRibosylation231trueSystems-wide analysis of serine-ADP-ribosylation reveals widespread occurrence and site-specific overlap with phosphorylation (part 2, phosphorylation data)Here we report systems-wide identification of serine ADP-ribosylation sites and quantification of their cellular changes in human cells upon oxidative stress. High-resolution mass spectrometry and unrestricted data processing confirmed that serine residues are the major target of ADP-ribosylation in HeLa cells. Proteome-wide analyses identified 3,090 serine ADP-ribosylation sites, with 97 percent of acceptor sites modulated more than 2-fold upon oxidative stress, while treatment with PARP inhibitor Olaparib abrogates induction (part 1, ADP-ribosylation data, PXD009208). Consistent with the nuclear expression of ARTD1/PARP1 and ARTD2/PARP2, serine ADP-ribosylation prominently occurred on nuclear proteins. Structural-predictive analyses revealed that serine ADP-ribosylation preferentially resides in disordered regions, and identified serine ADP-ribosylation sites to significantly overlap with known phosphorylation sites. Large-scale phosphoproteomics analysis supported these observations (part 2, phosphorylation data), hereby providing first evidence for site-specific crosstalk between serine ADP-ribosylation and phosphorylation. Collectively, we demonstrate that serine ADP-ribosylation is a widespread modification and a major nuclear responder to oxidative stress, and that its regulatory scope is comparable to other posttranslational modifications.2018-08-292018-05-28PXD009931280655164609918049829535851114514621333524422492156843179819354407110946194202452787284218809036809327042933084581058113633363515242601198343006636362758385696363NCBITaxon:12806888856189611143011872635147255571145263145262364934613640319520651269573666492391619746707385388364113546158215823106659034168895390531136364762721123294422291180995558323231225863654583334607925193659143205971293865581958511493152425911308322637371250232NCBITaxon:12721119577481866196821148858431043002451358672256807617554373592536684081691804542668342953195738264530586126683527877512010891498225058149636744521585545281132081479564100392375146369420494332089643795042089634459741379791387127283844006672032754532602935293644710671567593880364784346438677160521659940483216597216595455032309813152822165966930421659156271616968339162432183217546271292042779447623764543121810017203094980197525556978159509329629439843948840479NCBITaxon:215752533813152735515310510674398990371228577595152059687622623112108845555888201651645587077918178081736309431796758144571698936160488409859NCBITaxon:37162104782679401505907148364320421061320491381693630743277355543204663263331199456545674535110254111032174592161292886712642012886764100264203694569410167634102107612773515248676967491316582392547279334281461358430141729876091746334577444916754760489321287356756840301350621917265181301941119294393671104113167342810239320255598632582788396591047594120347495109716049016529982790513355252731336636666681024298036691758543980110243260866310245980813757757417555183201964471452680126757776414579663136701908323311561141553551042193255521138343965719276349818102697012359871069955529350701NCBITaxon:47518502983354761136157414654771800665564843376776020721441379299767982510216998238976414008208407414008211373511557186287011089725482444474153NCBITaxon:50557899698446752510003646534914862463341471474935480194675129911435974127647313708115466632654787656589363983532215958936698381920111067757727103481120837098523487804225326555414884142228638096640986147946409860995861571871766859919633715729513722117110129292117527110768783497419860202809134858513485845025392499148840633424951869123423515233598589859886559132497464998703920211107294331171139363527215987419085037117082711708282874341821144522350241451139692931902197822459390774636046147123747226940210195375939723287581721874202532566481219071NCBITaxon:573905079452659600131972211915853101774208588596321387NCBITaxon:61575052102830798951558641105198997237505998971246663576131720002631887634196102642661088878176179212190659407121906772275885815689964975151950582873756269072445066NCBITaxon:250682023717NCBITaxon:12065118703612201999857063446950605061724074767927450627249159615333554319313355418400524643213686150596487844612286662941786661347515363291018194301086731018510184NCBITaxon:176357303963558633149246437286287151458670580430801321650852718481865368595105135553482260710185687294296110091416333NCBITaxon:619184023210007348824123363630711218771047005812718787864515981289455564742163577553470951451225786425011449447260704NCBITaxon:1335626260705260707573061658457367734054163677483167233150561007133705654195NCBITaxon:3055504457913998781681190119287512987222829126806315327341478281055524NCBITaxon:9606135858469008171929111167815021979153283051930283672859300852900561742NCBITaxon:5693826883064017946991389959151011849952622543525875913151590141401294482428381113944225844160419370475927212044143708604232816772850351581990119519532590841267386370137024924053608838581155892622488394322936534036771861562277488175626158355837153716333760572671193501998086119350216787910857913711371272407190650371475917132912122662629812880571256274575727013290903090311333550372814243373857917851347924910078752824NCBITaxon:610111082NCBITaxon:961572433594633185862607991612432141262200298147802492670NCBITaxon:1263719281249212528303856838569591169266100658137353096322375612750454266625515197881356638164752037506442236695023600941293497905463224555253635247214012575151151997649908515151438992374546170374744544641522647324323593760264731592022288705158059553610019995550881579327160293851578114978633892479436293821162089453593647943750917389440NCBITaxon:68544127542251177529915901090134167387158955210129661030612967142368715851584515849157592376593934141038432715910022637673388914792359046142344423454670012876895911983329661091494467041464792936576164280341160221641331599280382803536909108917688149229990894621812141080348179392464028788929502711313329528282944568617493468191126526997227321890382102862107601247190400727NCBITaxon:991315973699782811431140206411981087135547743658142450722953379698204232124223129163218033698223470871453720916457332153581575462669402656194013716546759928777020206032811431933829314125295508332912924129221148643745725264999728303573908718308726727103790855071762997076789268370415573729214053156288765138829412816078395639243142626548982273775611592539059620732208659588273514312975516936053307972551811354914321371597495350261159556143213840607258781414473352150949334919496340161433472800593604631646104660762903381518454744850035670974772656691180771951240174841855601335755547920427415805360769946881037620148874162763870532972610059412003758289093164354282683142568500712303839900138946494083828351343074941442423221978542337742171598921409212205853939621157781154756929793244704749906604162103591905158114452749907312987779913112273170187546991103589915NCBITaxon:3729610360983342678181871049113079819643201336795316407783299467103401578658264530333733048526463830113064139925306423636505911696547885469811211620343176526161033728612994044586207340115357111952965261174553111471613386541313166343055213231201798360253237312761255232993512993850573163176426NCBITaxon:1108415099019794011513458993844577410632678728623219334151346186208621160791392227983516811961255228205623414953932232334774121827511676175731210807721348799994886185696281408434103351778275446677879963218496209948873213924882184932097115338209611533920953304393751441467569927612686266447NCBITaxon:26970493391120948509169862436941157183103121958832050920290610130685999758644458828643249952553324429724642606653324174115834390976821271789427863789426147740535608256737863912334355141998349317998686554055345144352914865451471245466553341889251042986357124679151641358257632884019373348649508771823803912903912952971962231274869317978911028688999513373105487866563632220341712516321389850762554701193806222523256318118781865713034431121308516558658862238NCBITaxon:4461144889198466649464546364931394586NCBITaxon:11072NCBITaxon:1107114280062421241873032883564913743751415167845881087439116597145943116598854331116781077854314408043933452703134NCBITaxon:110829534623226939773821899318226900351283875167114595311688533996695271067888695berry95418694200308954486972443662066563470150954530055862335518042643086922003022848124264281504751499105272831484201029910298471472414452386262903195418329025120847014658623195574974311700893062186441930611076323575584131016538235230805655772238235960393994636010660162360104399483994717974828679005853943965135444042376102127905688133775182363557211622962981767718705473693128160171121478610502382451855796694654318632051824066728297441739449323512450128853183889198679488531911137825591037164723327135166012615572283313146853152943649390NCBITaxon:10114284503163129780198573859523969393432379560260871213463959882674275295952664373343232235443142752422430814336195931334565826094327495925602916996311222336052016510871216979525283869728529046436804274212187073347472790101323429560312781611192194081882976058218381812498855733706312203401884397331274414163943755216203871072431398558588858725204936929554648038343691224326408172124193559552174939912816140559204254655194582288609183827275921274423408170193329829722759859557127442011431791274426235455272843552857382117627516481207294328413996166018762548296471876143786814904945142474801199150956481882297254572542725431761012148015412045514816721071031384738481882945190695150303416633603616598404849850569310151052835352835252702169373529451263753405206698743860186497405222972240521572477258594109767741856451804812310061911079450950NCBITaxon:81347800941269432271015227527943447256848479291146387033384991722617561094616143512032581205136521871448550523525944732054942334937347386437349527000651214466449917591891582580388043330376686244804612067811220260499NCBITaxon:129391080612461962902224619732063752299186705116941867057387350906834869121938791187947186802398007186801572307215451468357412581408186117571508471206777691838858032903116086113888691521677811472767212727909311036890100399784459724751295139870435123171912624696928123929486771333399522612627132521034304319629172644813817818219813936081882682108022815610054762411225575999265716329575535011123491922221979111196866693819791212471246148545104071328388281395348391080230294911040145954161934139036312041622110665890326441610511607111170227210411706488003127443210859562102831694128316669438376945597299495141065888271141131618790101058413308794784475174150808170539420654210556872933873174471270709964153292428141326303396211946691169608411454587932769537932920101984172931264690410661550537145755139251923239232014081576401316973135666160621213392830312383911283128224260512803395228110108328252535315285128112853353153498257419481478733959536231424101356742951056500242619145481NCBInr 20130519115062129459706473129282996339413469597538631211705128819351626539002134398031309800891496978103690122020718853225660173654856615664162425103695118702348334824688221187056386321219690226186129925016468606885656512623477702172195658101028166691265606156596560644329428386151330056717136043058991602358700500634349415048022160100298823578613252911187001447716522242157641809132529269975665566645246713488793796522392629161363363633661383366336721751117645126793406560103789059232146429314344870933114767468342808NCBITaxon:40559300181767465235909322641132065236526212633749821787074982161758316347256569056915692569343627873345073872249821165448720569734507611132794992899300166690510894491089448NCBITaxon:27592014538661410NCBITaxon:49326535871556895207108944410005896550873266897447094287535135248NCBITaxon:583330461130812637300692906530392665958114398872512117768726872387242665953872911786654987271352933134976724223110677410677330554831794417725784544417711581419198822445446NCBITaxon:1206638166611746731041059098829073249747344689960167767699444468587529600NCBITaxon:58114875211182263708248477154119165578428196578458484021194439553071580596566361640346241368108931610012686341428092644629612NCBITaxon:548681NCBITaxon:58205464143945033077553199211508579755228334875637379196627290581526009605960687594467087569603227086266596496043232595705093354812104543309013057375042929829387834810610010772675672675664306151279365431947448385449239470107445447211229844761191523191701610887679615NCBITaxon:129788266599348313969169287827573674544078211293145707529835103617120618925777128502289864803308352701262834854189854871427869991417158779627300251102856356304112673612599495291205286964696439644441768491146247274612874601134506963817152295263852639119813396569657302409757507639246573225264110909467469416348513151123869351746966222790935231523845NCBITaxon:56599557678421819211956233059173658220087932573135216742697969903461344346360513296440421345196789677124951533043479096109760616194032257329246200966927294227294327294451338968583554767894314696893420541280175576006465796811186969320111678078118694143618333038118698171947593217181717118691443906NCBITaxon:10298NCBITaxon:77712571183872715663384043306185969483641908027038476645687032573105687086182230547918678661833568851350NCBITaxon:7803906451515NCBITaxon:1502990316451516943129702982076211586121845121072573091930794968661094460111495791374944002092852013197470781384040529507619212638546146663915383379NCBITaxon:10239NCBITaxon:714173613095133434705470448300641751585923890037159084128108406972282334575412NCBITaxon:1024528104NCBITaxon:1024339201748378806109779706774811135622583314068636185509924286706527796583342409062004929102634949539321034809NCBITaxon:6312406741097842575460130761770707037661910094087074876138176510804148942329032176023993592672146752947474376032768917811214822126719285321773846451772177437411462272557104905615348119922512153239576769014NCBITaxon:7227156974726647091317047258520920269713032769785962859634114761309411470130813071263871214697212035332648373153214693383234114793981105268558370131469030713134937608333413118333357047285111114970396553972833321018418333121468745504510801874920026272415300910766206373735054114901042878280148517501303713444143983NCBITaxon:115523988139927515622435623593117928662623168390614404211945995677735119949016032932853422NCBITaxon:5759345089998103365786185336583517431275307796457425457428316273121536024271831627713528086913519948875058NCBITaxon:10407316275567802427155027799948716797182079541349660027130627415496753524502780291595412644323929158660025304292570212721398578135588675512349163349161296587930945804192031241358105151928434239549358153985804147174147181562698573011825902471491331678456099069027256027256122142725633544228410133562634166392867572279017904640459NCBITaxon:579653745757405741134676112335235623785483555222210457751262161310915755969154293959101364CP010395.1139622421392139018573232714896223923074196416402781288104896160106757445802402512296833529441394472036658117566144036612086605762661057634432206672400540296543NCBITaxon:11709140827428231772981843183443226224697657554992295757793694163857592845935561822810812845907066714367334442403531849223112105421152763910969762261111795752981882633148837318431922943815276409866613431241626528104977710442144386796267825317513278042676082490541565050511176946996NCBITaxon:1313102235171250795338942795588011987325197959621411408224430491408223264561421971014122872285186763185431252598520767737677742192530661294544580NCBITaxon:5571057041744726724042649329188912NCBITaxon:17732088932088952075591011766452315217531513463492316633687983020043674510116132842665672265872139269635889542301953188293883992271661581954666555325665788391943661230553411233681313590449822491155055438264131033799866694006884566684215767114353531066541151042983154689111298593119632756253232273075660469136165105612149165238976336316812149159704126308256913494091328881885513613NCBITaxon:1865381889083264241916073NCBITaxon:18653668730621017366041185650315750393765185165157157354571575111623427306855743680863772163157491529526689105767535862048867231223NCBITaxon:13886296230595975528400319758235857458429176160899458533668529170300273105231100911009040541009264653224323276056834062120079317860610645394778161312519821597397574692420975599802419947593744275775804227779415593362371955249131366239241073130368140731300146407662536670133888120410441568451111707111170869374515479221198561198572992014625944072NCBITaxon:5920165636713926767162481450134NCBITaxon:1043854084408771117454797787454610740162699412791542223131478196794967957018640814082380394528135340381659668872292612351786162432311008824323013599235612710876717589554978540964097978821035293052432776279129249451571155748409097804092409344713563429120253163761629838613147711573317810081631362515200223886585694415540181339717416870436486979656117762877323966920297936289931563430192234471376343387222795486634362991671306282334564331691736231259248586110936674464722138638842049321135472426419884794731447311210372696811129025516139812848131733367221440772179131693155577812211197782122152250255183610036NCBITaxon:11266523181860078NCBITaxon:43948811313231867944848514549414645637499010041715916922596345910659213334991065901249615334542NCBITaxon:6622432754427124327453655213401436495123901442701004971657167849641028941673688333295491249668425282652158109434326521577160197127908571622562237NCBITaxon:103572702NCBITaxon:1035811948816543605076011415784435590763200NCBITaxon:103591045856170721597653593913054927263480081795361795351859179534347834132234380221134458262762618903641969471395019825286349559839642711913645305312726212726232726241160719117718912389931214102460523121894840321714211821314269704940324803113180803049031417981305212483431114778749093238481437113153688918789184136841749408049804057882259319140243427149182837345979925613614179971408491154373429002813156713164163159403021009714629772823525174299246714467506013917261895192952492914505111524964922274849247496896731460519518766260201153348580029673015381862562269123280136032756142314222751190304143401191102214505204932101755275914284934266417990691417460911102051179777319795940825112111439190428245827621675391314639778518661390775271427350710619813530085592921486917198252145115772814144483407320369622328349593625NCBITaxon:116765807122962122413516343429110453754513678303086784952371094679895580158023627301574400000-0002-1439-3701