Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_57_1_III.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_MV_57_1_III.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_51_08_I.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_MV_51_08_I.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_51_08_III.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_MV_51_08_II.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_MV_51_08_III.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_51_08_II.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_57_1_I.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_MV_57_1_II.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_57_1_II.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/170823_JPC_MV_57_1_I.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/51_08_txt.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010389/57_1_txt.zipprimaryOK200001720pauline.cavanagh@uit.noJack-Ansgar BruunMass SpectrometryShotgun proteomicsNot availableStaphylococcus haemolyticusMembrane vesiclesSecretome.http://www.ebi.ac.uk/pride/archive/projects/PXD010389Bacterial strains and culture conditions S. haemolyticus blood culture isolate 51-08 from a neonatal sepsis, and S. haemolyticus commensal isolate (57-1) from the skin of a healthy adult were used. The bacteria were picked from blood agar plates, and grown overnight in Tryptic Soy Broth (TSB, Becton) at 37 °C. Overnight cultures were diluted 1:100 in 100 ml TSB (for isolation of proteins released by the bacteria into the supernatant) or in 1000 ml TSB (for isolation of membrane vesicles), and grown to OD600 of 2.0 ±0.2. Isolation of MVs from culture supernatants Bacterial cultures at OD600 of 2.0 ±0.2 were pelleted by centrifugation (5000g, for 20 min, at 4°C), and the supernatant was filtered through a 0.22 µm polyethersulfone membrane (Millipore express plus, Millipore, Molsheim, France), before being concentrated using Amicon Ultra-15, 100 kDa, centrifugation units (Milipore, Molsheim, France). Membrane vesicles were isolated by ultracentrifugation at 164, 326 x g (40 000 rpm, using SW 60 Ti rotor from Beckman Coulter, USA) for 2 hours at 4°C. The pellet was re-suspended in ice-cold Phosphate buffer saline (PBS), and further purified by gradient centrifugation. Thereto, the samples were mixed with OptiPrep TM (Sigma-Aldrich, Steinheim, Germany) to a final concentration of 30%, loaded to the bottom of a tube and further layered with 2000 µl 25% and 1000 µl 5% OptiPrep TM. The MV fractions were separated by density gradient centrifugation at an average of 84, 168 x g (30 000 rpm, using SW 50 Ti rotor from Beckman Coulter, USA) for 3h at 4°C. Fractions were then collected from the top. The presence of proteins in the different fractions was determined by SDS-PAGE followed by Coomassie staining (BioRad, Hercules, USA). OptiPrep fractions were analysed by transmission electron microscopy (TEM) for confirmation of MV content and sample purity. Further, fractions containing MVs were pooled and diluted in 4 ml PBS, before centrifugation using a Vivaspin 4 turbo tube (Sartorius, Germany) at 5000 x g for 30 minutes in order to remove residual OptiPrep solution. The sterility of the isolated MV samples was verified by streaking small aliquots on blood plates followed by incubation at 37°C overnight and further examined for growth of bacteria on the blood agar plate. All experiments were performed as three independent biological replicates. The various fractions in the gradient were evaluated for protein content by SDS-PAGE, and for presence of MVs by TEM analysis Bacterial cells were suspended in ultrapure water and placed on a freshly cleaved mica surface. The samples were incubated for approximately 5 min at room temperature and blotted dry before being placed into a desiccator. Representative images were collected by a Nanoscope V Atomic Force Microscope (Bruker AXS, Germany). Protein precipitation and sample preparation for mass spectrometry Proteins from culture filtrates and pooled MV fractions were precipitated. Protein pellets were washed five times with 70% ethanol and then incubated in 70% ethanol for 30 min at room temperature in a thermomixer at 600 rpm (Eppendorf, Germany). Finally, the pellets were washed once with 100% ethanol and dried in a speed vacuum centrifuge for 2 min. Subsequently, protein pellets were dissolved in a suitable volume of 8 M urea and incubated 1 h at room temperature in a thermomixer at 600 rpm (Eppendorf, Germany). Samples were centrifuged to remove potentially insoluble fractions and the supernatant was transferred to new tubes. Protein concentration was determined using the Qubit TM protein quantification kit (Thermo Fisher Scientific, USA). Four µg of protein was reduced and alkylated with Dithiothreitol and Iodoacetoamide, respectively, prior to digestion with a 1:20 ratio of trypsin (V511A, Promega, Wisconsin, USA). The resulting peptide mixture was purified and desalted using OMIX C18 tips (Varian, Inc., Palo Alto, CA, USA) and dried in a speed vacuum centrifuge. Dried peptides were dissolved in 0.2% formic acid. Mixtures of 2 µg peptides in 0.2% formic acid were loaded onto a Thermo Fisher Scientific EASY-nLC1000 system with an EASY-Spray column (C18, 2µm, 100 Å, 50µm, 50 cm). Peptides were fractionated using a 2-100% acetonitrile gradient in 0.1 % formic acid over 50 min at a flow rate of 250 nl/min. The gradient had four steps; an 8 min gradient step from 4 to 8% acetonitrile followed by a 50 min step from 8 to 40 % acetonitrile, a 8 min step to 100% acetonitrile and a final 8 min step at 100%. The separated peptides were analysed using a Thermo Scientific Q-Exactive mass spectrometer. Tandem mass spectra were collected in data-dependent acquisition (DDA) mode using a Top10 method.PrideLabel freeiodoacetamide derivatized residuemonohydroxylated residueThe raw data were processed by the MaxQuant software v1.6.0.16 using label-free quantification (LFQ) method. MS/MS data was were searched against the custom in-house databases of predicted protein sequences encoded by S. haemolyticus 51-08 (GCA_001230445.1 , 2527 entries) and S. haemolyticus 57-1 (sequence not published, 2518 entries). The false discovery rate (FDR) was controlled using a target-decoy approach and limited to 1%. The quantitative comparison of proteins from in the two different bacterial strains was performed using the relative iBAQ values (riBAQ) in Perseus programme v1.5.6.0, filtered for proteins with minimum two peptides identified. All contaminants were first filtered out and data were log10 transformed. Data were then analysed for statistically significant changes using t-test. Only proteins identified in at least two replicates in at least one of the two groups were considered. Missing values were replaced from normal distribution using width=0.3 and downshift=1.8 settings. Differentially expressed proteins were then visualized using Volcano plot with FDR<0.05 and artificial within group variance s0=0.3. For qualitative comparisons, only proteins present in at least two replicates in each group were considered further.ProteomicsJorunn Pauline CavanaghQ Exactive1. Department of Paediatrics, University Hospital of North Norway, Tromsø, Norway 2 Paediatric Research Group, Department of Clinical Medicine, Faculty of Health Sciences, UiT- The Arctic University of Norway, Tromsø, Norway.PARTIALStaphylococcus Haemolyticus30472255 Cavanagh JP, Pain M, Askarian F, Bruun JA, Urbarova I, Wai SN, Schmidt F, Johannessen M. Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate. J Proteomics. 2018 10.1016/j.jprot.2018.11.013jack-ansgar.bruun@uit.noBiologicalBiomedicalUniversity of TromsøNorwayStaphylococcus haemolyticus is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of S. haemolyticus are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other S. haemolyticus virulence factors is scarce. Bacterial membrane vesicles (MVs) are one mediator of virulence by enabling secretion and long distance delivery of bacterial effector molecules while protecting the cargo from proteolytic degradation from the environment. We wanted to determine if the MV protein cargo of S. haemolyticus is strain specific and enriched in certain MV associated proteins compared to the totalsecretome. The present study shows that both clinical and commensal S. haemolyticus isolates produce membrane vesicles. The MV cargo of both strains was enriched in proteins involved in adhesion and acquisition of iron. The MV cargo of the clinical strain was further enriched in antimicrobial resistance proteins. Data are available via ProteomeXchange with identifier PXD010389. BIOLOGICAL SIGNIFICANCE: Clinical isolates of Staphylococcus haemolyticus are usually multidrug resistant, their main virulence factor is formation of biofilms, both factors leading to infections that are difficult to treat. We show that both clinical and commensal S. haemolyticus isolates produce membrane vesicles. Identification of staphylococcal membrane vesicles can potentially be used in novel approaches to combat staphylococcal infections, such as development of vaccines.Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate.Cavanagh Jorunn Pauline JP, Pain Maria M, Askarian Fatemeh F, Bruun Jack-Ansgar JA, Urbarova Ilona I, Wai Sun Nyunt SN, Schmidt Frank F, Johannessen Mona Msodium salt, Sepses, ammonium formate, 13C-labeled, IL1BC, cadmium salt, MeCN, Diffraction Microscopy, CASP-1, chronic obstructive pulmonary disease (COPD), SDH, growth and development, Basodexan, magnesium formate, zinc salt, SDS, Polypeptides, protein polypeptide chains, KL receptor activity, Neonatal Late-Onset Sepsis, T22F8_160, cobalt(II) formate dihydrate, ATGPR7, CH3-C#N, GRP1, Cultural, Grp1, B1, Chronic irreversible airway obstruction, SCO5, Early-Onset Sepses, Urea, 1, GRP8, 2, GRP7, SCO1, Late-Onset Sepses, Analysis, Il1bc, Gsfsow3, adult, WMS, integumentum commune, insoluble, (R*, average, ethnicity, Divorced, Grain, sarcoma of breast, Analyses, rya-r44F, Bricd4, PTPSTEP, entire skin, CAFL - Chronic airflow limitation, composition, proteins, Divorces, W, PERB11.1, IL-1BC, Rya-R44F, AI047805, isolation and purification, Dehydrated ethanol, decreased, dehydrated, CG10844, Bacteria <bacteria>, column, method used in an experiment., disease (COPD), Prokaryotae, anatomical tube, infertility, sample, D1, Otf11, Fam91a1, Bs, 1110017I01Rik, Procaryotae, Neonatal Early-Onset, AV220772, skin and subcutaneous tissue, Histological Labeling, nickel salt, GPH, SGS, spiritus vini, chronic obstructive lung disease [Ambiguous], Caspase-1 subunit p10, BZRP, external covering of organism, cobalt (+2) salt, Electron Diffraction, integral to membrane, Test, Normalcy, breast sarcoma, Spectrum Analysis, DmelCG7788, PBS, Absolute Alcohol, ACMICD, PBR, CHRONIC OBSTRUCTIVE AIRWAY DIS, PBT, Neonatal Late-Onset Sepses, Cultural Background, sodium (4:1:1) salt, Cultures, Dm1, magnesium salt, Culture Test, prokaryotes, Sepsis, Airflow Obstruction, [OEtH], Early-Onset Sepsis, Hydrogen Oxide, GLYCINE-RICH PROTEIN 8, Transmission Electron, Chronic Obstructive Airways Disease, Chronic obstructive lung disease, pbt, Blood Cultures, CG11628, content, proportionality, formate, Spectrometry, pooled, rate, cromium (+3), Striatum-enriched protein-tyrosine phosphatase, Electron, beta Trypsin, alcohol etilico, Methylcarbinol, Painful bladder syndrome, F2G1.4, OBSTRUCTIVE PULMONARY DISEASE (COPD), duct, Drice, ammonium (4:1) salt, entire integument, methyl cyanide, DRICE, lead (+2) salt, Prokaryota, krk1, BPBS, Cleland Reagent, F10B6_15, region of skin, nickel formate dihydrate, GRP1/cytohesin 1, lead formate, sarcoma of the breast, Otf-11, DrIce, DrICE, etanol, aluminum salt, CAO - Chronic airflow obstruction, number, 4-dimercapto-, Carbamide, CHRONIC OBSTRUCTIVE PULM DIS, Chronic obstructive pulmonary disease NOS, R*)-, protein-containing complex, body system, CG7826, and RNA binding 1, F10B6.15, Chronic obstructive pulmonary disease finding, CG11633, Neonatal Early-Onset Sepses, surface, PULMONARY DISEASE (COPD), isolation, Gene Products, CG7835, CG42273, system, sterile, Carmol, Backgrounds, Skn-1a, portion of blood, Normalities, proportion, anatomical systems, Transmission, skin, 2310061K05Rik, thallium (+1) salt, ethanol, PCE-2, beta-Trypsin, rubidium salt, 4733401P19Rik, Cultural Relativisms, pk18, methanoic acid, SKIN, Turbo <genus>, MBR, Solution, skin organ, C2H5OH, Caspase-1 subunit p20, ATGRP7, ATGRP8, NEC, Customs, PTBR, Chronic, DmelCG10844, peptidos, PTHB1, Reproductive, proto-oncogene c-Kit, bacteria, DRR, Oct-11a, whole blood, 1-(14)C-labeled, chronic obstructive airways disease NOS, DRY, caspase 3, Electron Diffraction Microscopy, Proteins, COAD, MFT1, Clelands Reagent, skin zone, stepk, Stainings, potassium formate, CE-2, Mischung, copper (+2) salt, Cultural Backgrounds, compositionality, COLD - Chronic obstructive lung disease, acetonitrile, buffer, chronic, Cell, polypeptide, skin plus hypodermis, Neonatal Early-Onset Sepsis, density gradient, Temperatures, native protein, dry, carbonyldiamide, KIT ligand receptor activity, C18, cupric formate, chemical analysis, Strain, Infertile (finding), alcool ethylique, MFS1, infertility disorder, (COPD), chronic airway obstruction, chronic obstructive, aluminum formate, Skin, dJ142L7.2, CAL - Chronic airflow limitation, Mass Spectrum Analyses, XKrk1, Cleland's, AU020952, l(2)k00424, lithium formate, underdeveloped, Separations, GR-RBP7, postnatal growth, Neonatal Late Onset Sepsis, GR-RBP8, SWDS, IL-1 beta-converting enzyme, CDMT, ethanenitrile, Interleukin-1 beta-converting enzyme, CD117, mDRC, MFT, DmelCG11628, Epoc-1, ME-IV, plan specification, Cleland's Reagent, Gene Proteins, Blood Culture, l(2)k04913, Neonatal Sepses, C-Kit, Blood Culture Test, prokaryote, Ssm, Sterility, chronic obstructive lung disease (disorder), structure, xkl-1, quotient, Relativisms, phosphates, quantitative, Relativism, Cultural Relativism, chronic obstructive pulmonary disease, the integument, T22F8.160, Grain Alcohol, pelt, NCMe, determination, and rna binding 2, Skn-li, Blood, adult stage, Chronic Airflow Obstructions, postnatal development, zinc formate, CG7788, crice, lead salt, protein, Xkl-1, Pi, composed of, 1-hydroxyethane, BRICD4, alcohol, peptide, Background, Ethanol, Gsfsco1, ammonium tetraformate, ClvPrd, peptido, Alkohol, chronic obstructive airways disease NOS (disorder), Min, Chronic Obstructive Pulmonary Disease (COPD), protein aggregate, Gsfsco5, present in fewer numbers in organism, DmelCG42273, SOW3, Mass Spectrum Analysis, CYLDI, MIC-A, Eubacteria, COLD (chronic obstructive lung disease), kDa, peptides, NEC in ICD9CM_2006, Ethyl, E927b, membrane region, dermoid system, hypoplasia, force amplitude, min, mAPC, purification, IBP, Aethanol, copper, CYLD1, [CH2Me(OH)], Chronic airflow limitation, Ica, lithium salt, SOLO DANCERS, Sputolysin, ICE, GLYCINE RICH PROTEIN 7, l(2)k08110, dya, Strains, Bladder pain syndrome, glycine-rich RNA-binding protein 8, Sl, GPHYSD2, CHRONIC OBSTRUCTIVE PULMONARY DISEASE, ammonium (2:1) salt, ratio, CHRONIC, Tube, sterility, obstructive lung disease, Alcohol, ice, iCE, drice, USPL2, Dmel_CG7826, AI836084, Tr-kit, Labeling and Staining, organism surface, integumental organ, infertile, Ethyl alcohol, membranous organ component, Thermo Scientific, Airflow Obstructions, ur, Spectroscopy, pulmonary disease (COPD), Electron Microscopy, TUBE, carbamide, Cleland, drIce, drICE, low temperature, CHRONIC OBSTRUCTIVE, BC033609, chronic obstructive pulmonary disease and allied conditions, BRSS, 3.1.3.48, Chronic airway disease, kl1-A, NOS, KIT, ETHANOL, nickel (+2) salt, Adults, Dmel_CG7835, COPD NOS, Mnb, MNB, CHRONIC OBSTRUCTIVE LUNG DIS, tyrosine-protein kinase Kit, Chronic airway obstruction, Turbo <subgenus>, ammonium salt, Neonatal Late-Onset, membrane of organ, Painful Bladder Syndrome, adults, Neonatal Early Onset Sepsis, EtOH, cold, growth pattern, RyR, Step, non-developmental growth, Tests, P45, cobaltous formate, 3-Butanediol, kit, MASS, AW124434, Saint Pierre and Miquelon, mKIAA0493, organ system, Chronic Airflow Obstruction, H2NC(O)NH2, Culture Tests, vertebrate epidermis, Health, Labelings, phosphate, mICA, phosphate ions, integumentary system, RYR, Ryr, STEP, COPD - Chronic obstructive pulmonary disease, copper salt, chronic obstructive airways disease, p45, St. Pierre and Miquelon, Microscopy, Polypeptide, obstructive pulmonary disease (COPD), fertility disorders, Strains and Sprains, dermal system, COLD, chronic obstructive lung disease, 1728, Bladder Pain Syndrome, dermis plus epidermis plus hypodermis, Beliefs, Infertile, ryr, DISEASE (COPD), Reagent, Histological Labelings, cesium salt, CHM1L, SCF receptor activity, FBN, Gene, eubacteria, circadian rhythm, Spectrum Analyses, presence, epidermis, Buffer, formic acid, Karbamid, cytohesin/GRP1, Aethylalkohol, Cold, method, EAC, potassium salt, Pulmonary Disease, reduced, polypeptide chain, scfr, subnumerary, ECTOL1, integral component of membrane, 14C-labeled, Miquelon and St. Pierre, Harnstoff, RyRs, Mass, Histological, tiny, Sprains, Separated, SCFR, Mass Spectroscopy, Pro-Mega, ChM1L, Cold Temperatures, Fdc, Staining, Absolute, l(2)SH2 0323, decreased number, calcium formate, Chronic Obstructive, Monera, OCTD, Miquelon and Saint Pierre, Neural-specific protein-tyrosine phosphatase, Reproductive Sterility, region of membrane, cromium (+3) salt, fungi, culture, sodium formate, COPD, small, Neonatal, membrane, DYRK1, COAD - Chronic obstructive airways disease, data, nickel formate, DBI, 3H-labeled, protein complex, vertebrate blood, not elsewhere classified, Sprain, strontium formate, strontium salt, Labeling, PULM DIS CHRONIC OBSTRUCTIVE, Peptide, Culture, Chronic obstructive pulmonary disease finding (finding), Belief, development, membrane tetraspanin-enriched microdomain, count in organism, skin region, Oct11, Sterile, PKBS, natural protein, Protein, AI551343, whole membrane, Late-Onset Sepsis, Dyrk1, Skin-1a, CES2A1, GLYCINE-RICH RNA-BINDING PROTEIN 7, Vacuums, connected anatomical system, tegument, cell separation by density gradient centrifugation, l(2)SH0323, ACETONITRILE, Corsica, cyanomethane, WMS2, chronic obstructive airway disease, Ethyl Alcohol, CYH1, Temperature, Mass Spectrum, Separation, c-KIT, transmembrane, dRyR, Normality, uree, body surface, Dops, urea, CC1, vertebrate integument, Tripcellim, CGI-97, TeM, sample population, chromic formate, UREA, Protein Gene Products, Interleukin-1 beta convertase, Trypure, portion of skin, hydroxyethane, SBS, c-kit, integument, concentration, velocity, SSKS, 3.4.22.36, calcium salt, Blood Culture Tests, cold (chronic obstructive lung disease), Peptid, Chronic Obstructive Lung Disease, assay, growth, Transmission Electron Microscopy, TEM, presence or absence in organism, Rya-r76CDHorton's temporal arteritis, Bru, breadth, cephalosporin acylase activity, artificial sequence, Giant cell Arteritis, Raw, False, AI573844, number, Gene, protein, Computer, protein-containing complex, presence, inflammation of temporal artery, Normal Distributions, peptide, Polypeptides, method, protein polypeptide chains, Gaussian Distribution, arteritis cranialis, peptido, polypeptide chain, method used in an experiment, Gene Products, Horton’s syndrome, Giant Cell Arteritis, Del(8)44H, Software Engineering, Distribution, protein aggregate, Sprains, Computer Program, Application, Giant cell arteritis (disorder), Svc, Open Source Softwares, arteritis temporalis, proportion, F, reference sample, peptides, Software Application, Open, giant cell arteritis, Open Source Software, Computer Programs and Programming, proteins, Horton disease, present in organism, free, CA, Computer Software Application, Giant cell arteritis NOS (disorder), Tools, temporal arteritis, grupos, Normal, peptidos, Horton’s disease, Strains, Giant cell arteritis, Controlled, ratio, Applications Software, Open Source, data, Controlling, Computer Software, grupo, protein complex, T5E21.12, Proteins, Sprain, artificial gene, cranial arteritis, synthetic DNA, glutaryl-7-aminocephalosporanic acid acylase activity, Horton's disease, (7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase activity, Source Softwares, Peptide, predicted, Software Tools, Tool, group, Programs, polypeptide, Program, Computer Applications, count in organism, Software Tool, 7beta-(4-carboxybutanamido)cephalosporanic acid acylase activity, native protein, natural protein, Computer Applications Software, Protein, polymyalgia rheumatica, Strain, Distributions, Computer Applications Softwares, sequence, synthetic, group., Horton's giant cell arteritis, temporal artery inflammation, Softwares, cephalosporin C acylase activity, Software, Temporal arteritis (disorder), 5133401E04Rik, T5E21_12, Software Applications, Col4a-1, Source Software, Engineering, proportionality, Rest, rate, GA, synthetic constructs, primary structure of sequence macromolecule, plan specification, Horton's arteritis, Protein Gene Products, Computer Programs, Gene Proteins, Applications, GCA, SYNTHETIC CONSTRUCT sequences, Applications Softwares, GL-7-ACA acylase activity, quotient, GCL, artificial, Peptid, Polypeptide, glutaryl-7-ACA acylase activity, quantitative, Strains and Sprains, Gaussian, groupe, Gruppe, Computer Software Applications, presence or absence in organismmulticellular organismal catabolic process, single-organism catabolic process, dermis plus epidermis plus hypodermis, the integument, region of skin, Virulence, insensitive, pelt, exocrine gland fluid, antibiotique, antimicrobials, exocrine gland fluid or secretion, Otf-11, Skn-li, antimicrobial agents, Gene, protein, protein-containing complex, infectivity, cargo, Staphylococcus aureus strain 213 of Coagulase, epidermis, protein polypeptide chains, microbicides, polypeptide chain, integral component of membrane, Clumping Factor, Pathogenicity Factor, Gene Products, Impacts, 26Fe, Environmental Impacts, protein aggregate, Sprains, antibiotics, integumentum commune, Skn-1a, antimicrobial, study, viral infection, hierro, skin, catabolism, Antibiotika, membrane region, dermoid system, entire skin, Bodily, virus process, proteins, present in organism, SKIN, Staphylococcal, ecotype, Pathogenicity Factors, Environmental Impact, skin organ, secreted substance, Secretion, bodily secretion, Secretions, Otf11, Fam91a1, region of membrane, Strains, associated, AV220772, skin and subcutaneous tissue, Staphylocoagulase, Antibiotikum, antibiotic, membrane, Oct-11a, degradation, protein complex, Staphylococcus aureus strain 8325 4 of Coagulase, external secretion, Proteins, Sprain, fer, integral to membrane, skin zone, integumental organ, Factor, membranous organ component, Staphylococcal Clumping, Impact, strain, exocrine gland fluid/secretion, Environmental, Virulence Factor, Bodily Secretion, skin plus hypodermis, skin region, Oct11, Iron 56, native protein, natural protein, BC033609, Clumping Factor (Staphylococcal), Protein, Strain, Pathogenicity, whole membrane, microbicide, secretion, Staphylococcal Clumping Factor, cultivar, Skin-1a, tegument, Skin, Staphylococcus aureus strain 8325-4 of Coagulase, ferrum, transmembrane, membrane of organ, Factors, long distance, breakdown, resistant, Iron, Protein., vertebrate integument, Iron-56, mKIAA0493, Epoc-1, Fe, Protein Gene Products, Gene Proteins, portion of skin, vertebrate epidermis, Eisen, integument, Environments, Staphylococcus aureus clone pSCa2 of Coagulase, virulence, entire integument, iron, dermal system, exocrine gland secretion, Strains and Sprainsmulticellular organismal catabolic process, single-organism catabolic process, dermis plus epidermis plus hypodermis, the integument, region of skin, Virulence, insensitive, pelt, exocrine gland fluid, single-organism developmental process, antibiotique, antimicrobials, exocrine gland fluid or secretion, Otf-11, Skn-li, Infestations and Infections, antimicrobial agents, Gene, protein, biosynthesis, protein-containing complex, infectivity, cargo, Staphylococcus aureus strain 213 of Coagulase, epidermis, protein polypeptide chains, microbicides, polypeptide chain, integral component of membrane, Clumping Factor, Pathogenicity Factor, Gene Products, Staphylococcal Infections, Impacts, 26Fe, Environmental Impacts, protein aggregate, Sprains, antibiotics, integumentum commune, multicellular organismal biosynthetic process, Skn-1a, antimicrobial, study, viral infection, hierro, single-organism biosynthetic process, skin, Infections, formation, catabolism, Vaccine., anabolism, Antibiotika, membrane region, dermoid system, entire skin, Bodily, virus process, proteins, present in organism, SKIN, Staphylococcal, ecotype, synthesis, Pathogenicity Factors, Environmental Impact, skin organ, secreted substance, Infestation and Infection, Secretion, bodily secretion, Staphylococcus aureus Infections, Secretions, Otf11, Fam91a1, region of membrane, Strains, Infections and Infestations, associated, AV220772, skin and subcutaneous tissue, Staphylocoagulase, Antibiotikum, antibiotic, close to, membrane, data, Staphylococcus aureus Infection, Oct-11a, degradation, Staphylococcal Infection, protein complex, Staphylococcus aureus strain 8325 4 of Coagulase, external secretion, Proteins, Sprain, fer, integral to membrane, skin zone, integumental organ, Factor, membranous organ component, Staphylococcal Clumping, Impact, strain, near to, exocrine gland fluid/secretion, Environmental, development, Virulence Factor, Bodily Secretion, skin plus hypodermis, skin region, Oct11, Iron 56, native protein, natural protein, BC033609, Clumping Factor (Staphylococcal), Protein, Strain, Pathogenicity, whole membrane, Infection, microbicide, secretion, Staphylococcal Clumping Factor, cultivar, Skin-1a, Biofilm, tegument, Skin, Staphylococcus aureus strain 8325-4 of Coagulase, ferrum, transmembrane, membrane of organ, Factors, long distance, breakdown, resistant, Iron, vertebrate integument, Infection and Infestation, Iron-56, mKIAA0493, Epoc-1, Fe, Protein Gene Products, Gene Proteins, portion of skin, vertebrate epidermis, Eisen, integument, approaches, vicinity of, Environments, Staphylococcus aureus clone pSCa2 of Coagulase, virulence, entire integument, iron, dermal system, exocrine gland secretion, Strains and Sprains172trueComparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolateStaphylococcus haemolyticus is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of S. haemolyticus are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other S.haemolyticus virulence factors is scarce. Bacterial virulence is mediated by membrane vesicles (MVs) which enable secretion and long distance delivery of bacterial effector molecules while protecting the cargo from proteolytic degradation from the environment. We wanted to determine if the MV protein cargo of S.haemolyticus is strain specific and enriched in certain MV associated proteins compared to the totalsecretome. The present study shows that both clinical and commensal S. haemolyticus isolates produce membrane vesicles. The MV cargo of both strains was enriched in proteins involved in adhesion and in acquisition of iron. The MV cargo of the clinical strain was further enriched in antimicrobial resistance proteins.2018-11-302018-07-11PXD0103892806551646099180498511145422492156843544071284218327042363336351198343006636362758385696363121766668888111430155571145263145262364934613126957366649239161936413645106656889511363647627236611123294422295558326813042323122586365434607925193659106321432059712938655819125023254537111957748186619682158672256807617554373592536685862740816918045427877512010892250583674147956410039236942049433445974142001258641935293NCBITaxon:77643671567593643867716054048313152826930456271639162429204277944762376369612181003296292211376394863948840479NCBITaxon:215713152731051067125027820596875497792016516368407173630969893610478214836432042929410106132049138169374327732046640707453511110310780621745921612928867128867641006945694101676341021076127735113165821358430141729817463367541287356756840301350621813019411192943936711041136445913949843202530127032022149875110475941204788613474951537211097165299827905135551398031758543980126086639808137575551832019644719821118503114155421932555211383439119318198139818102697026635311069955529NCBITaxon:47518502983354764146547798251021691572739823897648407413735186287054824153NCBITaxon:505579844675251000364334147548021035974137081154665478121591498353221599838106771034819852348780422531488414222863226634794615718712593991599191572952929211752719860148840633424951869123423523815233598589859886559746499870392021110726399036352721598741908287439423241823502414511396929319025939071901746360123747463617226253600131972211915853105885969895110519899723798977200026341961026426672275885811927568996287375626907244202371720199985707240747679274596153335543193118734961671833554184005136861505968666211961748666134751536329NCBITaxon:17632862871638643110341103334883767058027184818653685959860757552747203610513529429611009363098402321000734882412336363071047005187878945556449447NCBITaxon:13356262664955419591399878168119011478281055524NCBITaxon:96063181781358584690081366848197915351930728593008526411409005118499526225412133759139014381113944235084370416744312044143708351581990119519532590841370137023943222774862615835583715371633376057267998086371137121906503714759172073127262981274575727090309031637910372814243371421044792491108239412NCBITaxon:96156588581478026926610065813735275045413566381647520375064422366950212934979054NCBITaxon:4468963224555253651997649908143899237453747264732376026473128870559553614169158586532527403271602938511497864794363755293824794372251177529912044741673872175091938441376593934347393271591002263767423444234546700128768959119846704146479293652803428038280351080348330779179392112621229502711311803738681911265269971028622527391076015973611432425071140981087135547743658142450722953379698204232124223321803369822130080347081645733185889716546759928777020382931412529550811481744888643745264999728303571830855071762997076789268370415156288765138856392456115293202086595551655181135491432137159749535026143213858781707683194963401614280053164686275110466038151845474485009747697477118051240174841855601555479204274381513553520148871105325441393297261005941289093164351211604990013894382835423242331082934122058560416299131122731701879915429131267818NCBITaxon:8175187104931640729946714423683033373304852646383011306413992530642369923121162065261610337286129940207340115357652611114716133865431201725323712552329935316317NCBITaxon:11084993841063267872862321933486208621160791392227681196125522814953932132232334774121827532917573129948861814084341033519963139248913924881153381153393304393755699286265599339112094886241571831046909975864442374864353324442606653324134390976821271786371979856863999839986865535291486541042986357358257632884019864982380111911028688999586652203411508599632138119380625631886571303443112130886581144889198466645463NCBITaxon:11072NCBITaxon:11071142800628835374375116597145943116598854331116781077854314408043933434NCBITaxon:11082693977381382189931824431935128387145953116885311213278695berry86941003335869724436647015011142869215047514991054842047147223540251208470146497444974311700893062186441930611076323308056603960162179748258539460553965132127905688133775129817663788760171121478610502366946573496320511245018974417394124501288531838891986794885319111379103716473516601529436316311985738969393432379560260871213463343232235443142752414336113345655602916996364368027901056031408188381857337034018812744149365304375521969697162038713985584081724055986091838272759212744234081702759812744201274426235455136565317627938211762753823943286303902474801199150956481882291690255120455148167238473848190695148498101510693731263753405203860186497405224052141856451804NCBITaxon:813478009152275484793870333849387191722617561094616143512032581205505235259447320549386411257226512165125388037668612067811220223074322011867051186705738733486938070312193879186802398007186801215451468351408186150847120677769183885160861138886915216778672127901008704351231719692812392948677133154845422612621981318826824112292657163295769381247124614854513283883483929491639590139036312041626658907715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