Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012321/mqpar.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012321/U373V3_KO1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012321/U373V3_KO2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012321/U373V3_KO3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012321/U373V3_WT2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012321/U373V3_WT1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012321/U373V3_WT3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012321/20161020_SP_HUMAN_R201609.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012321/txt.zipprimaryOK200fritz.roth@utoronto.caDae-Kyum KimMass SpectrometryShotgun proteomicsBrain Glioblastoma MultiformeSdcbpExtracellular vesiclehttps://www.ebi.ac.uk/pride/archive/projects/PXD012321BrainGlioblastThe purified EV protein preparation (9 μg) was desalted with SDS-PAGE loaded onto the stacking gel followed by staining and destaining. The in-gel trypsin digestion was carried out under reducing conditions afforded by DTT, and alkylation was achieved using iodoacetic acid. The lyophilized peptides were re-solubilized in 0.1% aqueous formic acid/2% acetonitrile, the peptides were loaded onto a Thermo Acclaim Pepmap (75 µm inner diameter 2 cm, C18, 3 μm particle size, 100 Å pore size) (Thermo Fisher Scientific, San Jose, CA) pre-column and the onto an Acclaim Pepmap Easyspray (75 µm inner diameter 15 cm, C18, 2 μm particle size, 100 Å pore size) (Thermo Fisher Scientific) analytical column. Separation was achieved using a Dionex Ultimate 3000 uHPLC at 220 nL/min with a gradient of 2-35% organic solvents (0.1% formic acid in acetonitrile) over 3 h. Peptides were analyzed using a Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific) operating at 120,000 resolution (FWHM in MS1, 15,000 for MS/MS) with higher-energy collisional dissociation sequencing of all peptides with a charge of 2+ or greater. All experiments were carried out in three biological replications.PrideWe used MaxQuant 1.5.6.5 with FDR cufoff of 0.05. We used the protein sequence from Swiss-Prot database (version R2016_09).ProteomicsFrederick P. RothDonnelly Centre, University of Toronto Lunenfeld-Tanenbaum Research Institute, Sinai Health SystemPARTIALHomo Sapiens (human)32296183 Luck K, Kim DK, Lambourne L, Spirohn K, Begg BE, Bian W, Brignall R, Cafarelli T, Campos-Laborie FJ, Charloteaux B, Choi D, Coté AG, Daley M, Deimling S, Desbuleux A, Dricot A, Gebbia M, Hardy MF, Kishore N, Knapp JJ, Kovács IA, Lemmens I, Mee MW, Mellor JC, Pollis C, Pons C, Richardson AD, Schlabach S, Teeking B, Yadav A, Babor M, Balcha D, Basha O, Bowman-Colin C, Chin SF, Choi SG, Colabella C, Coppin G, D'Amata C, De Ridder D, De Rouck S, Duran-Frigola M, Ennajdaoui H, Goebels F, Goehring L, Gopal A, Haddad G, Hatchi E, Helmy M, Jacob Y, Kassa Y, Landini S, Li R, van Lieshout N, MacWilliams A, Markey D, Paulson JN, Rangarajan S, Rasla J, Rayhan A, Rolland T, San-Miguel A, Shen Y, Sheykhkarimli D, Sheynkman GM, Simonovsky E, Taşan M, Tejeda A, Tropepe V, Twizere JC, Wang Y, Weatheritt RJ, Weile J, Xia Y, Yang X, Yeger-Lotem E, Zhong Q, Aloy P, Bader GD, De Las Rivas J, Gaudet S, Hao T, Rak J, Tavernier J, Hill DE, Vidal M, Roth FP, Calderwood MA. A reference map of the human binary protein interactome. Nature. 2020 580(7803):402-408 10.1038/s41586-020-2188-xdaekyum.kim@utoronto.caBiologicalUniversity of TorontoCanadaGlobal insights into cellular organization and genome function require comprehensive understanding of the interactome networks that mediate genotype-phenotype relationships^1,2. Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. With approximately 53,000 protein-protein interactions, HuRI has approximately four times as many such interactions as there are high-quality curated interactions from small-scale studies. The integration of HuRI with genome³, transcriptome⁴ and proteome⁵ data enables cellular function to be studied within most physiological or pathological cellular contexts. We demonstrate the utility of HuRI in identifying the specific subcellular roles of protein-protein interactions. Inferred tissue-specific networks reveal general principles for the formation of cellular context-specific functions and elucidate potential molecular mechanisms that might underlie tissue-specific phenotypes of Mendelian diseases. HuRI is a systematic proteome-wide reference that links genomic variation to phenotypic outcomes.A reference map of the human binary protein interactome.Luck Katja K, Kim Dae-Kyum DK, Lambourne Luke L, Spirohn Kerstin K, Begg Bridget E BE, Bian Wenting W, Brignall Ruth R, Cafarelli Tiziana T, Campos-Laborie Francisco J FJ, Charloteaux Benoit B, Choi Dongsic D, Coté Atina G AG, Daley Meaghan M, Deimling Steven S, Desbuleux Alice A, Dricot Amélie A, Gebbia Marinella M, Hardy Madeleine F MF, Kishore Nishka N, Knapp Jennifer J JJ, Kovács István A IA, Lemmens Irma I, Mee Miles W MW, Mellor Joseph C JC, Pollis Carl C, Pons Carles C, Richardson Aaron D AD, Schlabach Sadie S, Teeking Bridget B, Yadav Anupama A, Babor Mariana M, Balcha Dawit D, Basha Omer O, Bowman-Colin Christian C, Chin Suet-Feung SF, Choi Soon Gang SG, Colabella Claudia C, Coppin Georges G, D'Amata Cassandra C, De Ridder David D, De Rouck Steffi S, Duran-Frigola Miquel M, Ennajdaoui Hanane H, Goebels Florian F, Goehring Liana L, Gopal Anjali A, Haddad Ghazal G, Hatchi Elodie E, Helmy Mohamed M, Jacob Yves Y, Kassa Yoseph Y, Landini Serena S, Li Roujia R, van Lieshout Natascha N, MacWilliams Andrew A, Markey Dylan D, Paulson Joseph N JN, Rangarajan Sudharshan S, Rasla John J, Rayhan Ashyad A, Rolland Thomas T, San-Miguel Adriana A, Shen Yun Y, Sheykhkarimli Dayag D, Sheynkman Gloria M GM, Simonovsky Eyal E, Taşan Murat M, Tejeda Alexander A, Tropepe Vincent V, Twizere Jean-Claude JC, Wang Yang Y, Weatheritt Robert J RJ, Weile Jochen J, Xia Yu Y, Yang Xinping X, Yeger-Lotem Esti E, Zhong Quan Q, Aloy Patrick P, Bader Gary D GD, De Las Rivas Javier J, Gaudet Suzanne S, Hao Tong T, Rak Janusz J, Tavernier Jan J, Hill David E DE, Vidal Marc M, Roth Frederick P FP, Calderwood Michael A MAsodium salt, ammonium formate, 13C-labeled, Size, cadmium salt, MeCN, NCMe, PhrB photolyase activity, Particle, zinc formate, atado, lead salt, SDH, protein, magnesium formate, pigmented epithelium, Monosodium, zinc salt, Solvent, Alkylations, SDS, Polypeptides, ammonium tetraformate, peptido, cobalt(II) formate dihydrate, congenital, CH3-C#N, B1, 3, Min, NUP96, protein aggregate, epithelium, DmelCG42273, 2810441M03Rik, pore, peptides, Sodium Iodoacetate, pigmented retina, min, mAPC, CG12352, T6G21.3, copper, DNA cyclobutane dipyrimidine photolyase activity, lipomatosis of pancreas, SUPPRESSOR OF AUXIN RESISTANCE 3, AI047805, hNAT5, lithium salt, hSAN, column, SOLO DANCERS, D1, Shwachman-Bodian syndrome, ammonium (2:1) salt, DmAAF34715, Histological Labeling, nickel salt, Mak3p, PRE, Stars, NAT13, cobalt (+2) salt, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, Dmel_CG7826, AI836084, Labeling and Staining, Nat13, sodium (4:1:1) salt, magnesium salt, retinal pigment, Dm1, Sizes, dipyrimidine photolyase (photosensitive), nickel (+2) salt, retinal pigment layer, Acetic acid, Dmel_CG7835, Mnb, MNB, Acid, ammonium salt, formate, Shwachman-Diamond type metaphyseal dysplasia, cobaltous formate, Monosodium Iodoacetate, cromium (+3), Shwachman syndrome, AW124434, beta Trypsin, phr A photolyase activity, Labelings, DNA-photoreactivating enzyme, copper salt, ammonium (4:1) salt, Polypeptide, methyl cyanide, lead (+2) salt, STARS, 2600005K24Rik, F10B6_15, Particle Sizes, nat5, nickel formate dihydrate, lead formate, Monoiodoacetic Acid, Histological Labelings, aluminum salt, cesium salt, Gene, NAT5P, NAT13P, photoreactivating enzyme activity, protein-containing complex, F10B6.15, Monoiodoacetic, CG7826, formic acid, potassium salt, Ms1, 14C-labeled, Gene Products, CG7835, CG42273, Histological, Iodoacetic, stratum pigmentosa retinae, nat13, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, Staining, PRECOCIOUS, MS1, thallium (+1) salt, beta-Trypsin, rubidium salt, F23A5.3, 4733401P19Rik, calcium formate, methanoic acid, congenital lipomatosis of pancreas, male sterility 1, DmelCG12352, Iodoacetate, NAT5, Nat5, peptidos, cromium (+3) salt, sodium formate, F23A5_3, PTHB1, span, DYRK1, nickel formate, 1-(14)C-labeled, 3H-labeled, protein complex, mak3, Proteins, strontium formate, Stainings, potassium formate, copper (+2) salt, strontium salt, acetonitrile, Labeling, Peptide, AW112078, MODIFIER OF SNC1, native protein, C18, cupric formate, Protein, deoxyribonucleic photolyase activity, Sodium, Dyrk1, FWHM, aluminum formate, ACETONITRILE, cyanomethane, SAN, San, AU020952, lithium formate, RPE, PRSS, photolyase activity, MALE STERILITY 1 PROTEIN, CC1, Striated muscle activator of Rho-dependent signaling, Tripcellim, SWDS, Shwachman-Bodian-Diamond syndrome, pore-forming toxin activity, ethanenitrile, CGI-97, chromic formate, p. pigmentosa retinae, ME-IV, Protein Gene Products, san, Gene Proteins, Trypure, iodo-, Pancreatic insufficiency and bone marrow dysfunction, calcium salt, Peptid, deoxyribonucleate pyrimidine dimer lyase (photosensitive), Mak3, Peptide., channel-forming toxin activity, MAK3Vesicle, Exovesicles, SYCL, Sycl, MDA-9, Peptidomics, Apoptotic Body, Bodies, MDA9, TACIP18, Extracellular, Apoptotic, Apoptotic Bodies, ST1, Vesicles, Extracellular Vesicle, syntenin-1., Exovesicle, Body, microparticleProtein Gene Products, Gene Proteins, 3-(4-chlorophenylamino)-6-hydroxy-9-methyl-9H-carbazole-1, native protein, protein complex, Protein, Gene Products, Proteins, sequence, 4-dione., Gene, protein, protein aggregate, protein-containing complex, primary structure of sequence macromolecule, Data BaseSycl, Exovesicles, MDA9, Apoptotic Body, Bodies, TACIP18, Apoptotic Bodies, Vesicles, Extracellular Vesicle, syntenin-1, Body, microparticle, Concept, Vesicle, protein positioning, Role Concept, SYCL, Roles, MDA-9, protein recruitment, establishment of protein localisation, Extracellular, Role Concepts, Apoptotic, Role, Concepts, ST1, syntenin-1., Exovesicleother disease, scale tissue, human being, methionine aminopeptidase activity, Transcriptome Profile, FON1, peltate hair, Gene Expression Profile, organization and administration, Non-Governmental Organizations, Gene, SUPERMAN, protein, Profiles, FLORAL ORGAN NUMBER 1, protein-containing complex, Human, Readability, Homo sapiens, reduced, diseases, Roles, SUP, Gene Products, Concepts, disease or disorder, 2, diseases and disorders, Non-Governmental, tiny, protein aggregate, Man, familial adenomatous polyposis, MAP, peptidase M activity, multicellular organismal biosynthetic process, adenomas, Organization, rabGAPLP, single-organism biosynthetic process, L-methionine aminopeptidase activity, human disease, Man (Taxonomy), Genomes, familial adenomatous polyposis 2, plant peltate hair, Profile, Bdr, Tissue, hypoplasia, RabGAP-5, Super protein, Signatures, sp, Genotypes, SNAP-25, autosomal recessive multiple colorectal adenomas, non-neoplastic, Mif1, Non-Governmental Organization, Expression Signature, RUSC3, Role Concepts, Transcriptomes, disorder, Homo sapiens disease, Organizations, organization, small, Transcriptome, MUTYH-Associated Polyposis, organisation, organizational structure, protein complex, Modern, Proteins, Expression Profiles, MYH-associated polyposis, disorders, autosomal recessive familial adenomatous polyposis, Maps, total expressed protein, function, Synaptosomal-associated 25 kDa protein, medical condition, Concept, whole transcriptome, Gene Expression, Role Concept, native protein, Genogroup, Expression Signatures, Protein, Gene Expression Signatures, Proteomes., Diseases, Role, GENA70, condition, simple tissue, Gene Expression Signature, scales, Nongovernmental Organizations, Expression Profile, autosomal recessive, Transcriptome Profiles, administrative management, RUTBC3, scale, underdeveloped, Nongovernmental, administrative structure, FLO10, whole genome, Understanding, MYH-Associated Polyposis, HERP, human, RABGAP5, Protein Gene Products, Phenotypes, Gene Proteins, Genogroups, disease, Non Governmental Organizations, Gene Expression Profiles, SNAP, Modern Man, multiple colorectal, organizational management, MAP syndrome, Signature, Nongovernmental Organization, Proteomes, FAP2, FLORAL DEFECTIVE 10, colorectal adenomatous polyposisadenomas, RUTBC3, rabGAPLP, L-methionine aminopeptidase activity, human being, MUTYH-Associated Polyposis, Man (Taxonomy), methionine aminopeptidase activity, Protein., protein complex, familial adenomatous polyposis 2, Modern, Proteins, MYH-associated polyposis, autosomal recessive familial adenomatous polyposis, Maps, RabGAP-5, Gene, protein, MYH-Associated Polyposis, protein-containing complex, human, RABGAP5, autosomal recessive multiple colorectal adenomas, Human, Protein Gene Products, Gene Proteins, native protein, Homo sapiens, RUSC3, Modern Man, multiple colorectal, Protein, Gene Products, 2, MAP syndrome, protein aggregate, Man, familial adenomatous polyposis, MAP, peptidase M activity, FAP2, autosomal recessive, colorectal adenomatous polyposisfalseProteomics of extracellular vesicle after SDCBP knockoutTo see the role of SDCBP for protein recruitment into extracellular vesicle, we did comparative EV proteomic before and after SDCBP knockout.2019-12-022019-01-14PXD012321NEWT:3555NEWT:71647NEWT:35554NEWT:32046NEWT:544496NEWT:2042546NEWT:32049NEWT:259447NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:5722NEWT:1129NEWT:55153NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:10036NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:1147161NEWT:224326NEWT:1333499NCBITaxon:79857NEWT:1096976NEWT:95648NEWT:1589NEWT:135622NEWT:383379NEWT:10029NEWT:913645NEWT:317447NEWT:4688NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:4442NEWT:192875NEWT:59729NEWT:2164133NEWT:1316931NEWT:224308NEWT:3347NEWT:511145NEWT:931281NEWT:77133NEWT:145481NCBITaxon:79824NCBITaxon:4563NEWT:5755NEWT:44689NEWT:5759NEWT:1736231NEWT:1392NEWT:498217NEWT:2242NEWT:11320NEWT:286NEWT:391619NEWT:246196NEWT:287NEWT:246197NEWT:10239NEWT:44685NEWT:161934NEWT:1148NEWT:5508NEWT:5507NEWT:55571NEWT:35500NEWT:1140NEWT:1143NEWT:4896NEWT:1390NEWT:11557NEWT:1094343NEWT:1336795NEWT:644042NEWT:1773NEWT:1182590NEWT:3712NEWT:82380NEWT:935293NEWT:375146NEWT:263NEWT:52283NEWT:284812NEWT:8175NEWT:43330NEWT:1603293NEWT:408169NEWT:47946NEWT:3702NEWT:243277NEWT:7067NEWT:2850NEWT:408172NEWT:408170NEWT:3708NEWT:332648NEWT:536231NEWT:1436733NEWT:460519NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:9483NEWT:1513458NCBITaxon:40559NEWT:84588NEWT:480NEWT:67767NEWT:46835NEWT:109757NEWT:300852NEWT:1502NEWT:376686NEWT:95486NEWT:508771NEWT:1883446NEWT:253NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:93062NCBITaxon:4932NEWT:644223NEWT:235443NEWT:108458NEWT:272623NEWT:272624NEWT:983964NEWT:118499NEWT:32644NEWT:527796NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:476272NEWT:3747NEWT:195051NEWT:3988NEWT:1255228NEWT:649908NEWT:410289NEWT:373153NEWT:352472NEWT:1071661NEWT:360094NEWT:470NEWT:41364NEWT:1313NEWT:39491NCBITaxon:5811NEWT:29491NEWT:2014887NEWT:33952NEWT:571256NEWT:40483NEWT:9031NEWT:1872122NEWT:7091NEWT:108931NEWT:1055524NEWT:9778NEWT:150475NEWT:303NEWT:7111NEWT:347515NEWT:9534NEWT:5180NEWT:256737NEWT:9541NEWT:185579NEWT:941442NEWT:13076NEWT:7108NEWT:40479NEWT:317NEWT:1006581NEWT:885318NEWT:39488NEWT:550NEWT:4081NEWT:273068NEWT:554NEWT:98334NEWT:451516NEWT:36185NEWT:1225786NEWT:7574NEWT:1715256NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:6494NEWT:6491NEWT:507601NEWT:643680NCBITaxon:6157NEWT:6239NEWT:162425NEWT:216257NEWT:102169NEWT:9986NEWT:4054NEWT:1268063NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:28104NEWT:407821NCBITaxon:2NEWT:985076NEWT:1215323NEWT:986NEWT:52641NEWT:7159NEWT:28532NEWT:353152NEWT:27448NEWT:1194669NEWT:51329NEWT:243230NEWT:1080772NEWT:8479NEWT:6063NEWT:49240NEWT:104395NEWT:6289NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:725NEWT:469008NEWT:256318NEWT:206411NCBITaxon:6191NEWT:596153NEWT:1836NEWT:80863NEWT:105231NEWT:52638NEWT:4097NEWT:884204NEWT:9785NEWT:6279NEWT:1123869NEWT:9544NEWT:7370NEWT:83906NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:1134506NEWT:38783NEWT:4006NEWT:6426NCBITaxon:5476NEWT:6669NEWT:9935NEWT:89453NEWT:51515NEWT:51511NEWT:8845NEWT:92867NEWT:92866NEWT:5334NEWT:51750NEWT:202950NEWT:382352NEWT:413071NEWT:632957NEWT:9925NEWT:89462NEWT:8839NEWT:4232NEWT:2758385NEWT:11298NEWT:171101NEWT:714NEWT:421932NEWT:312017NEWT:196627NEWT:870435NEWT:5315NEWT:9913NEWT:105841NEWT:337330NEWT:2666255NEWT:2285NEWT:1392998NEWT:380394NEWT:981087NEWT:180066NEWT:226900NEWT:563041NEWT:326423NEWT:452467NEWT:36111NEWT:326424NEWT:1416333NEWT:1678078NEWT:749906NEWT:418985NEWT:749907NEWT:145263NEWT:694569NEWT:27606NEWT:9739NEWT:59202NEWT:9975NEWT:51953NEWT:3197NEWT:860688NEWT:13443NEWT:36329NEWT:62615NCBITaxon:3044782NEWT:72407NEWT:349741NEWT:157295NEWT:2096NEWT:9721NEWT:137221NEWT:9963NEWT:1617910NEWT:565050NEWT:2785785NEWT:411901NEWT:105884NCBITaxon:9615NEWT:58334NEWT:1193501NEWT:58331NEWT:6689NEWT:70448NEWT:698936NEWT:9940NEWT:521001NEWT:192011NEWT:1813887NEWT:224911NCBITaxon:11552NEWT:6945NEWT:184922NEWT:6703NEWT:2104NEWT:2NEWT:157546NEWT:474186NEWT:38942NEWT:307972NEWT:1496NEWT:2102NEWT:4513NEWT:1247NEWT:6938NEWT:376741NCBITaxon:10407NEWT:1736309NEWT:10360NEWT:47664NEWT:317548NEWT:3654NEWT:237561NEWT:5833NEWT:6928NEWT:36745NEWT:272563NEWT:82688NEWT:3885NEWT:3888NEWT:5821NEWT:44139NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:28909NEWT:515849NEWT:3641NEWT:466585NEWT:1000589NEWT:85963NEWT:85962NEWT:143361NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:34305NCBITaxon:183674NEWT:167387NEWT:84645NEWT:44586NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:431241NEWT:4550NEWT:1000561NEWT:2133NEWT:294381NEWT:197NEWT:1390363NEWT:288705NEWT:29176NEWT:4787NEWT:3218NEWT:556182NEWT:1270NEWT:374990NEWT:1432059NEWT:156471NEWT:4784NEWT:360106NEWT:156476NEWT:360104NEWT:10117NEWT:10116NEWT:1280NEWT:10358NEWT:1735272NEWT:83334NEWT:83332NEWT:317513NEWT:580240NEWT:294128NEWT:11676NEWT:100226NEWT:4530NEWT:75058NEWT:13616NEWT:172NEWT:296543NEWT:173NEWT:316435NEWT:42157NEWT:1895NEWT:1034304NEWT:105023NEWT:866628NEWT:215358NEWT:64152NEWT:4924NEWT:749200NEWT:9378NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:230741NCBITaxon:1313NEWT:44544NEWT:4911NEWT:645463NEWT:129249NEWT:1077286NEWT:943129NEWT:990119NEWT:1111708NEWT:227321NEWT:493760NEWT:106590NEWT:260710NEWT:257313NEWT:3827NEWT:400772NEWT:128161NEWT:930945NEWT:106592NEWT:46704NEWT:1187947NEWT:269796NEWT:10312NCBITaxon:1773NEWT:54571NEWT:9598NEWT:8030NEWT:1639NEWT:188229NEWT:3818NEWT:4909NEWT:759272NEWT:432359NEWT:1182263NEWT:943146NEWT:2711NEWT:537457NEWT:9103NEWT:29159NEWT:10306NEWT:8022NEWT:145943NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:29158NEWT:3635NEWT:5811NEWT:180923NEWT:411483NEWT:884019NEWT:198215NEWT:411490NEWT:169963NEWT:225117NEWT:746128NEWT:43665NEWT:1266464NEWT:367830NEWT:178616NEWT:375451NEWT:357NEWT:411469NEWT:84023NEWT:1679NEWT:559292NEWT:411464NEWT:411460NEWT:2762NEWT:1174673NEWT:1328426NEWT:562NEWT:411470NEWT:2094720NCBITaxon:2697049NCBITaxon:138NEWT:28038NEWT:1663NEWT:1423NEWT:4932NEWT:3603NEWT:2759NEWT:3847NEWT:38293NEWT:1428NEWT:327159NEWT:178876NEWT:1660NEWT:327160NEWT:573NEWT:571NEWT:241368NEWT:42528NEWT:2664373NEWT:190802NEWT:9417NEWT:1216979NEWT:7237NEWT:115104NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:220668NEWT:1821314NEWT:1249668NEWT:9646NEWT:5286NEWT:7227NEWT:7469NEWT:9402NEWT:9644NEWT:415540NEWT:675060NEWT:334542NEWT:313625NEWT:27592NEWT:426428NEWT:63459NEWT:1276815NEWT:588858NEWT:9639NCBITaxon:11071NEWT:242231NEWT:197221NEWT:7215NEWT:2172103NEWT:7460NEWT:434271NEWT:747679NEWT:42752NEWT:746360NEWT:89764NEWT:470150NEWT:543734NEWT:73239NEWT:38018NEWT:226186NEWT:8782NEWT:115357NEWT:435590NEWT:1902NEWT:1908NEWT:13164NEWT:216129NEWT:2664179NEWT:623NEWT:7038NEWT:6192NCBITaxon:38727NEWT:2829NEWT:366581NEWT:216599NEWT:216595NEWT:40559NEWT:8355NEWT:633NEWT:9685NEWT:7029NEWT:1283300NEWT:186990NEWT:632NEWT:6183NEWT:630NEWT:334747NEWT:61235NEWT:343691NEWT:15368NEWT:1491466NEWT:436486NEWT:51655NEWT:170187NEWT:260707NEWT:185431NEWT:29760NEWT:260704NEWT:1719NEWT:703612NEWT:260705NEWT:692672NEWT:2697049NEWT:1216981NEWT:7004NCBITaxon:6073NEWT:1089448NEWT:1329349NEWT:607699NEWT:208963NEWT:208964NEWT:27337NEWT:575584NEWT:8727NEWT:8726NEWT:10090NEWT:4120NEWT:5693NEWT:2186NEWT:8724NEWT:8723NEWT:37334NCBITaxon:10359NEWT:1203258NEWT:242619NEWT:49317NEWT:160791NEWT:34073NEWT:373995NEWT:5689NEWT:544404NEWT:37349NEWT:4113NEWT:837NEWT:36015NEWT:5691NEWT:48479NEWT:61435NEWT:627025NEWT:1097677NEWT:375939NEWT:61674NEWT:1117957NEWT:6526NEWT:8709NEWT:4100NEWT:1159556NEWT:5671NEWT:1076NEWT:4101NEWT:6763NEWT:803NEWT:29722NEWT:1692259NEWT:1486917NEWT:419947NEWT:468911NEWT:817NEWT:7604NEWT:818NEWT:135588NEWT:1843183NEWT:5661NEWT:29719NEWT:58002NEWT:6997NEWT:452589NEWT:4577NEWT:5664NEWT:2157NEWT:146479NEWT:1042986NEWT:1911079NEWT:69373NEWT:206189NEWT:264203NEWT:1480154NEWT:1274414NEWT:9612NEWT:38626NEWT:38865NEWT:3077NEWT:9615NEWT:10299NEWT:112273NEWT:1147787NEWT:1440772NEWT:1355477NEWT:9605NEWT:9606NEWT:90371NEWT:641501NEWT:4153NEWT:7668NEWT:915099NEWT:74940NEWT:13551NEWT:9600NEWT:1450511NEWT:1143193NEWT:174934NEWT:2823525NEWT:9838NEWT:9839NEWT:3055NEWT:4146NEWT:5478NEWT:6326NEWT:5476NEWT:1450520NEWT:1042311NEWT:299767NEWT:28835NEWT:1274432NEWT:2053667NEWT:1274426NEWT:9825NEWT:1274423NEWT:393765NEWT:39946NEWT:9823NEWT:95602NEWT:8732NEWT:314539NEWT:39947NEWT:718274NEWT:1274420NEWT:57845832296183