Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944/p1.2.mzXMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944/Controle1Candida.mzXMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944/p3.1.mzXMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944/Controle2Candida.mzXMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944/p4.1.mzXMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944/p3.2.mzXMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944/p4.2.mzXMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944/p1.1.mzXMLftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012944/txt.zipprimaryOK200psbmendonca2@uem.brGlaucia AritaMass SpectrometryShotgun proteomicsNot availableCandida albicansSystemic candidiasisVirulenceProteomicsSerial passageLc-ms/msHost–pathogen interactionMass spectrometryhttp://www.ebi.ac.uk/pride/archive/projects/PXD012944The serial systemic candidiasis consisted of initially infect female Balb/c mice with an inoculum of 3.5x105 wild-type C. albicans SC5314 cells (WT) via lateral tail vein. After five days post-infection, the animals were euthanized. The kidney was aseptically removed, homogenized in lysis buffer (200 mM NaCl, 5 mM EDTA, 10 mM Tris, 10% glycerol v/v, pH 8.30), plated on SDA and incubated for 24 h at 35 °C. Colonies recovered were used to prepare inoculum for the subsequent infections as described for WT, totalizing five serial passages (P1-P5). Furthermore, these colonies recovered from kidney were submitted to protein extraction. Then, cells were collected and centrifuged for 5 min at 3000 rpm. The pellet was washed three times with ultrapure water and resuspended in 1100 μL of buffer containing 7 M urea, 2 M thiourea, and 40 mM DTT. Then, the suspension was sonicated (30 bursts of 10 seconds ON, 30 seconds OFF with 100% amplitude). After that, the lysate was centrifuged at 4800 rpm for 10 min at 4 °C, and supernatant collected and stored at -80 °C. Bradford assay was performed for protein quantification, using bovine serum albumin (Sigma) as a standard. Prior to digestion, protein content was denatured with 8M urea, then, reduced with 5 mM dithiothreitol (DTT) and alkylated with 14 mM iodoacetamide (IAA). For the quench of the remaining IAA, 5 mM DTT was added. Samples were diluted with four volumes of 50 mM ammonium bicarbonate and 1 mM calcium chloride was added with subsequent trypsin (V5280 - Promega) digestion at 37 °C overnight (ratio of 1 μg enzyme to 100 μg protein). Then, samples were acidified with 0.4% trifluoroacetic acid to stop trypsin action and desalted using SepPak tC18 cartridges (WAT 036820) before subjection to mass spectrometry.PrideLabel freeiodoacetamide derivatized residuemonohydroxylated residueSamples were analyzed by LC-MS/MS using ultra-high performance liquid chromatography (Shimadzu, Nexera X2, Japan) coupled to high resolution mass spectrometry (Impact II, Bruker Daltonics Corporation, Germany) and equipped with an electrospray ionization source. Thus, ten microliter of the sample were loaded at flow of 0.1000 ml/min. The capillary voltage was operated in positive ionization mode, set at 4500 V and with an end plate offset potential of −500 V. The dry gas parameters were set to 7.0 L min−1 at 180 °C with a nebulization gas pressure of 2.0 bar. Data were collected from m/z 150-2200 with an acquisition rate of 5 spectrums per second, and the ions of interest were selected by auto MS/MS scan fragmentation. Chromatographic separation was performed using gradient A containing purified water and 0.1% formic acid (v/v) and B containing acetonitrile with 0.1% formic acid (v/v) using a C18 column (Shim-pack GIST - 3um x 1.0 mm x 250 mm). The gradient used was 2%, 35%, 95%, 95% and 2% of B in 2, 60, 90, 110 and 111 min respectively, and stopped at 120 min. Files obtained by the mass spectrometer were normalized using Data Analysis software (Bruker, Germany), converted in a vendor free file by MSFile from the ProteoWizard Platform and processed in MaxQuant software (version 1.6.2.3). The proteins were searched against reference proteome from C. albicans (strain SC5314 / ATCC MYA-2876) taxonomy code 237561 obtained from UniProt Database. Parameters were set for digestion mode specific for trypsin with maximum of two missed cleavage sites. Variable modifications were oxidation (M) and acetyl (Protein N-term) and fixed modification was carbamidomethyl (C) with maximum of five modifications. False discovery rates (FDR) were set to 0.01 for both peptide and protein levels. Then, MaxQuant output file was imported in Perseus (version 1.6.1.3) for statistical analysis. First, protein dataset were filtered, removing proteins into categorical parameters: contaminant, only identified by site and reverse database. For statistical analysis, the level of stringency was set protein identification with LFQ values greater than zero in two of the replicates. Thus, data analysis was performed using two sample test, considering significant those proteins with - log10 (p value < .05) associated with the highest difference (passage versus WT). All proteins were classified according to molecular functions and/or biological processes obtained from UniProt (https://www.uniprot.org/) and Candida Genome Database (http://www.candidagenome.org/). Furthermore, proteins were separated according to their role in virulence factors expressed by C. albicans.ProteomicsPatrícia de Souza Bonfim de Mendonçaimpact IIDepartment of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringá, BrazilPARTIALCandida Albicans (yeast)glauciasayuria@gmail.com31293987 Arita GS, Meneguello JE, Sakita KM, Faria DR, Pilau EJ, Ghiraldi-Lopes LD, Campanerut-Sá PAZ, Kioshima ÉS, Bonfim-Mendonça PS, Svidzinski TIE. Serial Systemic Candida albicans Infection Highlighted by Proteomics. Front Cell Infect Microbiol. 2019 9:230 10.3389/fcimb.2019.00230BiomedicalUniversidade Estadual de MaringáBrazil<i>Candida albicans</i> is the major pathogen isolated from nosocomial bloodstream infections, leading to higher mortality rates. Thus, due to its clinical relevance, studies aiming to understand host-pathogen interactions in <i>C. albicans</i> infection are necessary. Therefore, we performed proteomic analysis using a murine model of serial systemic infection by <i>C. albicans</i> to evaluate possible changes in the protein profile of the pathogen over time. Firstly, we observed a reduction in the median survival time of infected animals with increasing passage number, suggesting a higher pathogenicity acquired during repeated infections. By LC-MS/MS, it was possible to obtain protein profiles from the wild-type strain (WT) and compare them to proteins extracted from <i>Candida</i> cells recovered from infected tissues during passages one, three, and four (P1, P3, and P4). We obtained 56, 29, and 97 proteins in P1, P3, P4, respectively, all varying in abundance. Regarding biological processes, the majority of proteins were related to carbohydrate metabolism, stress responses and amino acid metabolism. The proteins were also categorized according to their potential role in virulence traits, such as biofilm production, yeast-to-hyphae transition, phenotypic switching, proteins related to stress responses, and uncharacterized proteins. Therefore, serial infection in combination with proteomic approach enabled us to deepen the existing knowledge about host-pathogen interactions.Serial Systemic <i>Candida albicans</i> Infection Highlighted by Proteomics.Arita Glaucia Sayuri GS, Meneguello Jean Eduardo JE, Sakita Karina Mayumi KM, Faria Daniella Renata DR, Pilau Eduardo Jorge EJ, Ghiraldi-Lopes Luciana Dias LD, Campanerut-Sá Paula Aline Zanetti PAZ, Kioshima Érika Seki ÉS, Bonfim-Mendonça Patrícia de Souza PS, Svidzinski Terezinha Inez Estivalet TIELaboratory, Mus domesticus, Ethylenediaminetetraacetic acid, metazoans, A4, Infestations and Infections, CASP-14, protein, Basodexan, 2-(indol-3-yl)ethanoic acid, lysis, composed of, Candida <Debaryomycetaceae> infection, House Mouse, (ethylenedinitrilo)tetraacetic acid, post-vent region vein, Trifluoroacetate, protein polypeptide chains, STOP, halite, Glycerol, IAA, Wolffian adenoma, 1, Urea, BALB/cJ, 2, Min, 2', Analysis, protein aggregate, present in fewer numbers in organism, systemic candida infections, DmelCG42273, Edetic acid, Animalia, (R*, Mass Spectrum Analysis, 3-Propanetriol, C, 2-diyldinitrilo)tetraacetate, Oelsuess, ion(4-), vein of post-vent region, Analyses, N, E927b, hypoplasia, min, Swiss Mice, mAPC, composition, proteins, Wolffian adnexal tumor, AI047805, cloruro sodico, salt, decreased, FATWO, Sputolysin, scientific observation, trifluoro-, disseminated candidiasis, Systemic candida infections, Infections and Infestations, autolysin activity, necrosis, (ethane-1, acidum edeticum, reniculate kidney, ratio, Glyceritol, ethylenediaminetetraacetate, rock salt, lateral, mouse, 2'', Dmel_CG7826, {[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID, infections, female, Spectrum Analysis, ur, Spectroscopy, any method, carbamide, common salt, (Indol-3-yl)acetate, Dm1, Cleland, female adnexal tumor of probable Wolffian origin, Mini-ICE, glycerol, bacteriocin activity, disseminated candida, Mtap6, IES, Dmel_CG7835, Acetic acid, Hydrogen Oxide, Mnb, MNB, Mus musculus, Acid, Gro, Caspase-14 subunit p10, glycerine, WAT, mice, BALBc, content, Swiss Mouse, proportionality, Trifluoroacetic, Spectrometry, Infection and Infestation, rate, Caspase-14 subunit p19, 3-Butanediol, N'-1, AW124434, MICE, natrii chloridum, lysed material, beta Trypsin, domesticus, Calcium Chloride, Kidneys, H2NC(O)NH2, Indole-3-acetic acid, DMDA, lysin activity, heteroauxin, Cesium, Acetamide, Calcium chloride, bacteriolytic toxin activity, Mouse, Wolffian adnexal neoplasm, 1728, Cleland Reagent, 3.4.22.-, GLYCEROL, Reagent, 4-dimercapto-, mini-ICE, Carbamide, ethylenediaminetetraacetic acid, Spectrum Analyses, R*)-, protein-containing complex, animals, TYPE, EDTA tetraanion, CG7826, Buffer, DAGA4, Karbamid, Glycerine, glycyl alcohol, Propanetriol, Stable tubule-only polypeptide, polypeptide chain, reduced, House, subnumerary, Acide ethylenediaminetetracetique, Trihydroxypropane, Harnstoff, CG7835, Mass, Mus musculus domesticus, CG42273, Animal, tiny, Carmol, MAM, Mice, SCG3, acide edetique, Mass Spectroscopy, Pro-Mega, Cesium Trifluoroacetate, proportion, 2'''-(ethane-1, vein of tail, 2-Ethane diylbis-(N-(carboxymethyl)glycine), propane-1, STOP145, Swiss, ethylenediamine tetraacetic acid tetraanion, Glycerin, disseminated candidosis, beta-Trypsin, chlorure de sodium, Indoleacetic acid, decreased number, study assay, Infestation and Infection, edta, 2-diyldinitrilo)tetraacetic acid, lysate, glycerolum, systemic candidiasis, invasive candidiasis, (indol-3-yl)acetic acid, Suspension, EDTA, Kochsalz, Anhydrous, 2-iodo-, small, measuring, 1H-indol-3-ylacetic acid, DYRK1, 2810411E12Rik, female human body, Candida infection, protein complex, Glyzerin, Map-6, 3-triol, Clelands Reagent, compositionality, buffer, Cell, acido edetico, LGMD2C, native protein, Natriumchlorid, natural protein, Mus, carbonyldiamide, thrush, Metazoa, Protein, Infection, table salt, 145-kDa STOP, Dyrk1, NaCl, female adnexal tumour of probable Wolffian origin, Mass Spectrum Analyses, MAP-6, Cleland's, AU020952, 3-Trihydroxypropane, Mass Spectrum, holin, DMDA1, underdeveloped, Calcium Chloride Dihydrate, uree, urea, CC1, H4edta, 3-Indolylessigsaeure, Tripcellim, House Mice, UREA, Laboratory Mice, caudal vein, ME-IV, multicellular animals, Cleland's Reagent, Trypure, Candidosis, Wolffian tumour, SCARMD2, structure, quotient, Mass., Candida <Debaryomycetaceae>, edetic acid, Wolffian tumor, Laboratory Mouse, Females, sodium chloride, ethylenediaminetetraacetate tetraanionPeptidomics., Endomyces albicans, Infestation and Infection, Procandida stellatoidea, Saccharomyces albicans, Dematium albicans, Oidium albicans, Procandida albicans, Candida stellatoidea type I, Mycotorula albicans, Infection, Infestations and Infections, Candida stellatoidea, Candida albicans var. stellatoidea, Monilia stellatoidea, Infection and Infestation, Infections and Infestations, Syringospora albicans, Parasaccharomyces albicans, Myceloblastanon albicans, Monilia albicanssodium salt, projections, bar-h1, ammonium formate, 13C-labeled, Virulence, cadmium salt, MeCN, ion, gant, bar, Mya, Softshell Clams, magnesium formate, zinc salt, gastrointestinal stromal sarcoma, 5730420M11Rik, Polypeptides, protein polypeptide chains, cobalt(II) formate dihydrate, CH3-C#N, B1, HRR-1, Barh1, gastrointestinal stromal tumor, germacrene A synthase activity, Software Engineering, Analysis, Softshell Clam, SET, Divorced, F, Analyses, Genomes, rya-r44F, phosphatase 2A inhibitor I2PP2A, proteins, Divorces, ecotype, Rya-R44F, AI047805, DmelCG4299, PA1, set, dehydrated, CG10844, column, sample, D1, Role Concepts, s, RIP14, arenarias, nickel salt, B2AR, NK/GPI, LC-MS2, Gpi, Bonin Islands, anatomical protrusion, Lindnera jadinii, HRR1, Data Set, cobalt (+2) salt, Liquid Chromatography, familial, GAST, Spectrum Analysis, CG4637, Software Tools, Cyberlindnera jadinii, Computer Applications, sodium (4:1:1) salt, Role Concept, Dm1, magnesium salt, BH1, Computer Applications Software, shelf, Role, Pathogenicity, NK|GPI, hrr1, END, 6-trans-farnesyl-diphosphate diphosphate-lyase (germacrene-A-forming) activity, Hydrogen Oxide, Software Applications, HLA-DR-associated protein II, DI-2, Source Software, I-2Dm, proportionality, formate, Spectrometry, rate, cromium (+3), inhibitor of granzyme A-activated DNase, beta Trypsin, projection, ridge, I-2PP1, Applications, TAF-IBETA, liquid chromatography-tandem mass spectroscopy, ammonium (4:1) salt, TAF-Ibeta, methyl cyanide, lead (+2) salt, Bglap-rs1, hg, Computer Software Applications, HH, nickel formate dihydrate, lead formate, False, region or site annotation, lamellae, aluminum salt, 2-trans, thomson, protein-containing complex, process of organ, CG5529, CG7826, Hh, mass-to-charge ratio, stromal tumor of gastrointestinal tract, lamella, Softshell, RNF47, Saccharomyces jadinii, LC-MSMS, CG7835, Gene Products, Pathogenicity Factor, CG42273, Pgi, Application, Open Source Softwares, proportion, positional, thallium (+1) salt, Gpi-1r, Software Application, Gpi-1s, Phi, rubidium salt, isolated cases, beta-Trypsin, Gpi-1t, Open Source Software, 2pp2a, methanoic acid, ESI, ions, CG10574, Torulopsis utilis, Computer Software Application, Mya arenarias, 2PP2A, Tools, xfor, bar-3, dSET, dSet, DmelCG10844, peptidos, laminae, PTHB1, DRR, 1-(14)C-labeled, DRY, barh1, anatomical process, Proteins, Candida utilis, potassium formate, Factor, copper (+2) salt, MF, acetonitrile, (+)-(10R)-germacrene A synthase activity, Amf, Tool, Concept, polypeptide, hrr-1, DmelCG5529, native protein, I-2PP2A, dry, C18, cupric formate, Dm I-2, barH1, aluminum formate, Mass Spectrum Analyses, organ process, NK, AU020952, FXR, l(2)k00424, lithium formate, Separations, ethanenitrile, ME-IV, fxr, Computer Programs, Gene Proteins, processes, l(2)k04913, Applications Softwares, binding_or_interaction_site, Hansenula jadinii, anon-WO0182946.19, Torulopsis, quotient, liquid chromatography tandem mass spectrometry, Torula utilis, ADRB2R, gastrointestinal stromal neoplasm, Data Analyses, liquid chromatography tandem mass spectroscopy, IPP2A2, NCMe, zinc formate, cleavage, lead salt, protein, Mya arenaria, Bar H1, binding site, Pichia jadinii, peptide, BETA2AR, ammonium tetraformate, anon-WO0134654.19, DmelCG4637, ClvPrd, peptido, Roles, Concepts, Min, Mir, protein aggregate, Gpi-1, Computer Program, DmelCG42273, Mass Spectrum Analysis, LCMSMS, peptides, TAF-I, Nlk, Open, min, Computer Programs and Programming, mAPC, Clam, µl, copper, SCAN, free, Pathogenicity Factors, IGAAD, lithium salt, DmelCG10574, papilla, dya, C16orf53, blood capillary, associated, ammonium (2:1) salt, ratio, phapii, (+)-germacrene A synthase activity, gastrointestinal stromal tumor (gist), rip14, Monilia, LC-MS/MS, lamina, Dmel_CG7826, StF-IT-1, flanges, Th, ul, Source Softwares, Programs, Spectroscopy, Program, Virulence Factor, mOC-X, Ionen, ADRBR, bar3, Computer Applications Softwares, cultivar, Softwares, GAS, nickel (+2) salt, Dmel_CG7835, region, Mnb, MNB, ammonium salt, ORG, Org, l(3)neo56, Factors, l(3)neo57, positional polypeptide feature, gastrointestinal stromal tumors, RyR, shelves, Monilias, cobaltous formate, Gpi1-r, Gpi1-s, CG4299, gas, AW124434, Myas, Gpi1-t, GIST, RYR, Ryr, spine, copper salt, gist, Polypeptide, i2pp2a, Proteomes, Gpi1s, protein levels, ryr, AI461847, cesium salt, Gene, Spectrum Analyses, Computer, PHAPII, LC-MS-MS, formic acid, protrusion, potassium salt, Bar-H1, template-activating factor I, polypeptide chain, 14C-labeled, RyRs, Mass, Separated, Mass Spectroscopy, iones, Mrt, ipp2a2, ridges, calcium formate, taf-ibeta, site, cromium (+3) salt, HHT1, sodium formate, Clams, statistical analysis, Applications Software, Open Source, DYRK1, l(3)hh, data, Computer Software, nickel formate, 3H-labeled, protein complex, 6-trans-farnesyl-diphosphate diphosphate-lyase [(+)-germacrene-A-forming] activity, igaad, strontium formate, total expressed protein, strontium salt, Peptide, strain, LC/MS/MS, Software Tool, capillary vessel, natural protein, Protein, I2PP2A, Dyrk1, Software, ACETONITRILE, flange, cyanomethane, Data Base, Mass Spectrum, Separation, dRyR, INSDC_feature:misc_binding, CC1, Candida guilliermondii var. nitratophila, Engineering, Tripcellim, sample population, chromic formate, Protein Gene Products, Trypure, process, dSET/TAF-Ibeta, 2610030F17Rik, Virulence., Pressures, Ion, Data, calcium salt, Bar, processus, BAR, Peptid, ORW1, variable, AA407739, BarHI, Rya-r76CDliquid chromatography tandem mass spectroscopy, projections, biochemical pathways, Pyohemia, Pyemias, Metabolic Process, Virulence, Procandida stellatoidea, Blood, Aminosaeure, Metabolic Concepts, metazoans, A4, Infestations and Infections, Amino acid, Monilia stellatoidea, protein, toxemia, lysis, symbiotic process, post-vent region vein, Pyaemias, protein polypeptide chains, Roles, responsivity, POIS BLOOD, Concepts, encompassing mutualism through parasitism, Pyemia, Metabolism Concept, protein aggregate, Phenomenon, Animalia, LCMSMS, amino acids, vein of post-vent region, Candida stellatoidea type I, catabolism, proteins, metabolic process resulting in cell growth, Pathogenicity Factors, reaction, papilla, Role Concepts, biotransformation, Infections and Infestations, autolysin activity, necrosis, associated, Catabolism, Parasaccharomyces albicans, reniculate kidney, LC-MS2, symbiotic interaction between organisms, anatomical protrusion, Blood Poisoning, Process, lateral, Aminokarbonsaeure, metabolism resulting in cell growth, LC-MS/MS, lamina, flanges, Candida albicans var. stellatoidea, Severe Sepsis, Syringospora albicans, alpha-amino carboxylic acids, Virulence Factor, Role Concept, Procandida albicans, shelf, Role, Pathogenicity, bacteriocin activity, NOS, secretion, Hypha, Biofilm, Sepsis, Poisoning, Amino Acid, Acid, Yeast, Epistemology, carbohydrate metabolism, Factors, Amino acids, Oidium albicans, Blood Poisonings, shelves, Infection and Infestation, projection, ridge, Endomyces albicans, Kidneys, DMDA, lysin activity, Poisonings, spine, Pyaemia, liquid chromatography-tandem mass spectroscopy, bacteriolytic toxin activity, Candida stellatoidea, Acids, Myceloblastanon albicans, parasitism, lamellae, Processes, Aminocarbonsaeure, Gene, commensalism, alpha-amino acid, Metabolic Processes, protein-containing complex, Septicemias, animals, process of organ, TYPE, LC-MS-MS, Buffer, protrusion, DAGA4, lamella, Septicemia, polypeptide chain, Dematium albicans, Metabolism, LC-MSMS, single-organism carbohydrate metabolic process, Gene Products, Pathogenicity Factor, Animal, symbiosis, MAM, SCG3, Metabolism Phenomena, Monilia albicans, study, reactivity, multicellular organismal carbohydrate metabolic process, symbiotic interaction between host and organism, vein of tail, BLOOD POIS, Metabolic Concept, ridges, Hyphas, Infestation and Infection, phenotypic dimorphism, Mycotorula albicans, laminae, degradation, protein complex, anatomical process, Proteins, alpha-amino acids, Factor, Systemic infection (disorder), buffer, Cell, LGMD2C, Concept, Metabolic Phenomena, Metabolism Concepts, LC/MS/MS, native protein, natural protein, Metazoa, Protein, Phenomena, Infection, metabolism, flange, organ process, Metabolic Phenomenon, multicellular organism metabolic process, holin, Saccharomyces albicans, DMDA1, biodegradation, Metabolic, symbiotic interaction, Amino, Carbohydrate, caudal vein, Protein Gene Products, host-pathogen interaction, multicellular animals, Gene Proteins, processes, process, single-organism metabolic process, symbiotic interaction between species., Septicaemia, Pyohemias, SCARMD2, liquid chromatography tandem mass spectrometry, processus, response, Severe, Systemic infection, Anabolismliquid chromatography tandem mass spectroscopy, projections, biochemical pathways, Pathogen Interaction, Metabolic Process, Procandida stellatoidea, Host Pathogen Relations, Aminosaeure, Metabolic Concepts, metazoans, Infestations and Infections, A4, Amino acid, Monilia stellatoidea, protein, infectivity, Long Term, Pichia jadinii, Case Fatality Rates, Pathogen-Host Interaction, Death Rates, protein polypeptide chains, Pathogen Host Interactions, Roles, Concepts, AW048865, Excess Mortalities, Metabolism Concept, protein aggregate, Phenomenon, Effect, Host-Pathogen Relation, Pathogen Interactions, Animalia, viral infection, LCMSMS, amino acids, Crude Mortality Rates, Candida stellatoidea type I, catabolism, Tissue, proteins, metabolic process resulting in cell growth, virus process, ecotype, Host Pathogen Interactions, reaction, CFR Case Fatality Rate, papilla, Role Concepts, biotransformation, Infections and Infestations, Age-Specific Death Rate, Catabolism, Long-Term Effects, Parasaccharomyces albicans, LC-MS2, Lindnera jadinii, anatomical protrusion, Process, Monilia, Aminokarbonsaeure, metabolism resulting in cell growth, LC-MS/MS, lamina, Crude Death, Longterm Effect, flanges, Candida albicans var. stellatoidea, Syringospora albicans, not genetically inherited, alpha-amino carboxylic acids, Crude Mortality, Cyberlindnera jadinii, Mortality, Interaction, Role Concept, Procandida albicans, shelf, Pathogenicity, Role, secretion, cultivar, Hypha, Biofilm, Amino Acid, Acid, Yeast, Epistemology, carbohydrate metabolism, death rate, Amino acids, Oidium albicans, Host-Pathogen, shelves, Monilias, Relation, Mortalities, Infection and Infestation, projection, ridge, Host-Pathogen Interaction, Endomyces albicans, Mortality Rate, Crude Death Rate, DMDA, spine, liquid chromatography-tandem mass spectroscopy, Festa, Candida stellatoidea, Acids, Mortality Determinant, time, Myceloblastanon albicans, Effects, FESTA-L, lamellae, Processes, FESTA-S, Mortality Declines, Aminocarbonsaeure, Excess, Gene, Crude Mortality Rate, alpha-amino acid, Metabolic Processes, protein-containing complex, mortality measurement, animals, process of organ, TYPE, LC-MS-MS, Age-Specific Death, protrusion, Mortality Determinants, DAGA4, Crude, period, lamella, Rate, Determinant, Host-Pathogen Relations, Saccharomyces jadinii, polypeptide chain, Dematium albicans, Relations, Metabolism, Host Pathogen Interaction, LC-MSMS, single-organism carbohydrate metabolic process, Gene Products, Case Fatality, Animal, MAM, SCG3, Metabolism Phenomena, U19, Monilia albicans, Differential Mortality, BM040, multicellular organismal carbohydrate metabolic process, Excess Mortality, Longterm, Host., Metabolic Concept, ridges, Long-Term, Age-Specific, Torulopsis utilis, Hyphas, Infestation and Infection, phenotypic dimorphism, Mycotorula albicans, Mortality Rates, mortality rate, Crude Death Rates, Host Pathogen, Long-Term Effect, laminae, Age Specific Death Rate, Mortality Decline, degradation, protein complex, anatomical process, Traits, Proteins, Candida utilis, alpha-amino acids, Cell, LGMD2C, strain, Concept, Metabolic Phenomena, Case Fatality Rate, Metabolism Concepts, LC/MS/MS, survival, native protein, natural protein, Pathogen-Host Interactions, Protein, Long Term Effects, Metazoa, proteomic analysis, Phenomena, Infection, Determinants, Death, metabolism, flange, organ process, Metabolic Phenomenon, Rates, Death Rate, multicellular organism metabolic process, Saccharomyces albicans, DMDA1, biodegradation, Metabolic, Pathogen-Host, Candida guilliermondii var. nitratophila, Age-Specific Death Rates, Amino, Carbohydrate, TRAITS, Longterm Effects, Protein Gene Products, multicellular animals, Gene Proteins, processes, process, Differential, single-organism metabolic process, Hansenula jadinii, Host, SCARMD2, Torulopsis, Decline, liquid chromatography tandem mass spectrometry, processus, virulence, Pathogen Host Interaction, Torula utilis, Interactions, Differential Mortalities, AnabolismPyohemia, Pyemias, Procandida stellatoidea, Saccharomyces albicans, Blood Poisoning, Oidium albicans, Candida stellatoidea type I, Blood Poisonings, Blood, Candida albicans var. stellatoidea, BLOOD POIS, Monilia stellatoidea, Severe Sepsis, Systemic infection (disorder), NOS., Syringospora albicans, toxemia, Septicemias, Endomyces albicans, Pyaemias, Septicemia, Poisonings, Dematium albicans, Pyaemia, Procandida albicans, Septicaemia, Mycotorula albicans, POIS BLOOD, Pyohemias, proteomic analysis, NOS, Candida stellatoidea, Pyemia, Sepsis, Poisoning, Parasaccharomyces albicans, Severe, Systemic infection, Myceloblastanon albicans, Monilia albicansfalseProteomic analysis of Candida albicans after serial systemic infection in murine modelThis study assessed proteomic profile of Candida albicans after serial systemic infection in a murine model. The animals were infected initially by wild-type C. albicans SC5314 (WT) with an inoculum of of 3.5x105 cells via lateral tail vein. Then, five days post-infection, the animals were euthanized and their kidneys were removed, homogenized in lysis buffer, plated on SDA and incubated for 24 h at 35 °C. Colonies recovered from infected kidney were used to prepare inoculum for the subsequent infections as described for WT, totalizing five serial passages (P1-P5) and they were also used to protein extraction. By LC-MS/MS, 479 proteins were identified, with 56 proteins statistically significant in abundance in P1, 29 proteins in P3 and 97 proteins in P4. Regarding biological processes, the majority of proteins were related to carbohydrate metabolism, stress response and amino acid metabolism. The proteins were also categorized according to their potential role in virulence factors such as biofilm production, yeast-to-hyphae transition, phenotypic switching, proteins related to stress response and uncharacterized proteins. Therefore, serial infection associated with proteomic approach enabled to deepen the knowledge about host-pathogen 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