Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/proteinGroups.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/summary.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/parameters.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/msms.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/peptides.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/proteinGroups-Copy.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/experimentalDesignTemplate.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/evidence.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction8.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction12.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction7.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction15.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction12.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction15.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction14.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction7.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction9.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction15.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction14.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction8.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction8.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction12.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction14.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction9.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction9.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate2-Fraction7.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate1-Fraction11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD013590/SILACBiologicalreplicate3-Fraction6.rawprimaryOK200gux2002@suda.edu.cnDan LiMass SpectrometryShotgun proteomicsStomach CarcinomaNot availableHumanSilac quantificationOrbitrap eliteLc-ms/msMgc803Ufbp1http://www.ebi.ac.uk/pride/archive/projects/PXD013590StomachGastric Cancer Cell LineSample processing protocol Construction of stable cell lines: Lentiviral particles expressing pLVX-IRES-zsGreen1 and pLVX-UFBP1-FLAG-IRES-zsGreen1 were used to infect MGC803 cells. The cells were sorted out by flow cytometry to obtain the control or UFBP1-expressing cells. SILAC labeling: The control cells (MGC803-pLVX) were grown in light medium (containing light lysine K0: 12C6, 14N2 and light arginine R0: 12C6, 14N4) and the UFBP1-expressing cells (MGC803-UFBP1) were grown in heavy medium (containing heavy lysine K8: 13C6, 15N2 and heavy arginine R10: 13C6, 15N4). Cells were cultured for more than six passages to ensure the complete incorporation of heavy arginine and lysine. In-solution digestion and fractionation: Same number of cells from control and experimental samples were mixed, lysed, and digested with Lys-C and trypsin after reduction and alkylation. The resulting peptide samples were fractionated with a C18 column and each fraction was concentrated in a vacuum centrifugal concentrator (CentriVap, Labconco, USA), desalted with C18 ZipTip (Millipore, USA), concentrated again, and dissolved in 0.1% formic acid. LC-MS/MS analysis: LC-MS/MS analysis was performed on an LTQ-Orbitrap Elite hybrid mass spectrometer. Purified peptides in buffer A (0.1% formic acid) were loaded to the enrichment column and then separated in an analytical column with a 150 min linear gradient of buffer B (80% acetonitrile and 0.1% formic acid) at a flow rate of 200 nL/min. Mass spectra were acquired in a positive-ion mode with an automatic Xcalibur data system. The experiment was performed for three biological replicates.PrideSILACiodoacetamide derivatized residueisotope labeled residuemonohydroxylated residueRaw MS data were searched using MaxQuant (Version 1.6.1.1) against a human protein UniProt database (released in July 2017). The precursor and fragment ion mass tolerances were set to 10 ppm and 0.6 Da, respectively. Cysteine carbamidomethylation was set as fixed modification while methionine oxidation and heavy arginine and lysine (Arg10 and Lys 8) were set as variable modification. The maximum of two trypsin missed cleavages were allowed and only peptides with ≥ 7 amino acids were included for the identification. The false discovery rate (FDR) for peptides was set to 1%, which was determined by searching a reversed decoy database. Relative protein abundance H/L ratio was obtained from the intensities of heavy peptides to light peptides from the experimental and control samples.ProteomicsGuoqiang XuLTQ Orbitrap ElitePARTIALCollege of Pharmaceutical Sciences,Soochow University,ChinaHomo Sapiens (human)33219317 Hu Z, Wang X, Li D, Cao L, Cui H, Xu G. UFBP1, a key component in ufmylation, enhances drug sensitivity by promoting proteasomal degradation of oxidative stress-response transcription factor Nrf2. Oncogene. 2021 40(3):647-662 10.1038/s41388-020-01551-1445220956@qq.comSoochow UniversityChinaThe key component in the UFM1 conjugation system, UFM1-binding and PCI domain-containing protein 1 (UFBP1), regulates many biological processes. Recently it has been shown that low UFBP1 protein level is associated with the worse outcome of gastric cancer patients. However, how it responses to the sensitivity of gastric cancer to chemotherapy drugs and the underlying molecular mechanism remain elusive. Here, we discovered that high UFBP1 expression increases the progression-free survival of advanced gastric cancer patients treated with platinum-based chemotherapy. Cell-line based studies unveiled that UFBP1 expression enhances while UFBP1 knockdown attenuates the sensitivity of gastric cancer cells to cisplatin. High-throughput SILAC-based quantitative proteomic analysis revealed that the protein level of aldo-keto reductase 1Cs (AKR1Cs) is significantly downregulated by UFBP1. Flow cytometry analysis showed that UFBP1 expression increases while UFBP1 knockdown reduces reactive oxygen species upon cisplatin treatment. We further disclosed that UFBP1 attenuates the gene expression of AKR1Cs and the transcription activity of the master oxidative stress-response transcription factor Nrf2 (nuclear factor erythroid-2-related factor 2). Detailed mechanistic studies manifested that UFBP1 promotes the formation of K48-linked polyubiquitin chains on Nrf2 and thus augments its proteasome-mediated degradation. Experiments using genetic depletion and pharmacological activation in vitro and in vivo demonstrated that UFBP1 enhances the sensitivity of gastric cancer cells to cisplatin through the Nrf2/AKR1C axis. Overall, this work discovered a novel prognostic biomarker for gastric cancer patients treated with platinum-based chemotherapy and elucidated the underlying molecular mechanism, which may benefit to future personalized chemotherapy.UFBP1, a key component in ufmylation, enhances drug sensitivity by promoting proteasomal degradation of oxidative stress-response transcription factor Nrf2.Hu Zhanhong Z, Wang Xiaohui X, Li Dan D, Cao Lindong L, Cui Hongxia H, Xu Guoqiang Gmulticellular organismal catabolic process, single-organism catabolic process, DNA Oxidative, transcription factor, CG13826, Oxidative Stress, T-cell leukemia, anon-WO0153538.7, anon-WO0153538.6, Antioxidative, AGL4, Antioxidative Stress, Nitrative Stress, SEPALLATA 2, Damage, l(3)j5E7, Stresses, DmelCG43286., zinc ion regulated core promoter proximal region sequence-specific DNA binding, Oxidative Nitrative, Oxidative DNA, Oxidative, Stress Injury, E4TF1-60, Dmel_CG13826, responsivity, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, Nitro-Oxidative Stress, CG4566, Oxidative Cleavages, NFT2, Nitro-Oxidative Stresses, sequence-specific DNA binding, Oxidative Injury, CNC, Cnc, DNA Damage, Oxidative Damages, reactivity, Oxidative Injuries, Injury, catabolism, Oxidative Cleavage, F10N7_150, NRF2, Nrf2, RCH04A07, Oxidative DNA Damages, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, Anti-oxidative, Oxidative Stress Injuries, Oxidative Stresses, Anti oxidative Stress, Oxidative DNA Damage, RNA polymerase II proximal promoter sequence-specific DNA binding, Antioxidative Stresses, copper ion regulated proximal promoter sequence-specific DNA binding, AGAMOUS-like 4, zinc ion regulated proximal promoter sequence-specific DNA binding, Dmel_CG17894, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, 1110001I20Rik, TRANSCRIPTION FACTOR, RNA polymerase II distal enhancer sequence-specific binding, RNA polymerase II core promoter proximal region sequence-specific binding, degradation, Ufbp1, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Anti-oxidative Stresses, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, dJ1187M17.3, l(3)03921, NRF2A, transcription factor activity, Oxidative Nitrative Stress, BcDNA:RE05559, Oxidative and Nitrosative Stress, Oxidative Nitrative Stresses, RNA polymerase II transcription factor activity, CG43286, DNA Oxidative Damages, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, C20orf116, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, UFBP1, CG17894, F14P3.4, breakdown, Anti-oxidative Stress, DNA Oxidative Damage, F14P3_4, AI326138, CG4578, CNC_DROME, sequence-specific DNA binding RNA polymerase II transcription factor activity, Cleavage, metal ion regulated core promoter proximal region sequence-specific binding, copper ion regulated core promoter proximal region sequence-specific binding, 2600009E05Rik, Transcription factor, F10N7.150, Nitro-Oxidative, AI194320, 5134, Stress, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, Oxidative Stress Injury, E4TF1A, DNA, response, Nitro Oxidative Stress, metal ion regulated sequence-specific DNA binding, metal ion regulated proximal promoter sequence-specific DNA binding, Oxidative Damage, sequence-specific transcription regulatory region DNA bindingsodium salt, liquid chromatography tandem mass spectroscopy, ammonium formate, 13C-labeled, cadmium salt, MeCN, NCMe, ion, Lysine Hydrochloride, determination, Experiment., L-arginine, DL-Arginine Acetate, r10, zinc formate, Monohydrate, lead salt, Visible Light, magnesium formate, zinc salt, Alkylations, peptide, Polypeptides, Lysine Acetate, ammonium tetraformate, ClvPrd, peptido, cobalt(II) formate dihydrate, CH3-C#N, hnu, B1, Line, 2, Min, Flow Microfluorimetry, Arg, Cell Sortings, foton, WMS, DmelCG42273, L Lysine, (S)-2-amino-5-guanidinopentanoic acid, Divorced, LCMSMS, reference sample, peptides, Fluorescence Activated Cell Sorting, K, (S)-2-Amino-5-guanidinovaleric acid, R, epsilon-diaminocaproic acid, min, Arginine, mAPC, Monohydrate DL-Arginine Acetate, Divorces, number of, copper, AI047805, lithium salt, LYS, column, Lysin, sample, D1, GPHYSD2, ammonium (2:1) salt, Flow Cytofluorometry, gamma, nickel salt, SGS, L-Arginin, LC-MS2, Cell Sorting, Fluorescence-Activated Cell Sorting, Radiation, cobalt (+2) salt, Trypsin/K, Arginine Hydrochloride, LC-MS/MS, Dmel_CG7826, arginine, Light, extra or missing physical or functional parts, dJ1187M17.3, not genetically inherited, ACMICD, Microfluorimetry, sodium (4:1:1) salt, LIGHT, magnesium salt, Dm1, Ionen, lysine, nickel (+2) salt, Dmel_CG7835, Lines, Visible Radiations, L-Arginine, Mnb, MNB, ammonium salt, Cytofluorometries, Visible Radiation, Ring 10, HVEML, formate, cobaltous formate, AI326138, cromium (+3), alpha, MASS, AW124434, study protocol, Fluorescence-Activated Cell, beta Trypsin, 2600009E05Rik, MGC803, experimental procedures, organ system, light quantum, liquid chromatography-tandem mass spectroscopy, copper salt, ammonium (4:1) salt, Polypeptide, methyl cyanide, lead (+2) salt, nickel formate dihydrate, experimental, lead formate, aluminum salt, cesium salt, number, L Isomer, FBN, high weight, body system, CG7826, LC-MS-MS, Ly113, L-(+)-arginine, formic acid, Buffer, Fluorescence-Activated, method, Fluorescence-Activated Cell Sortings, Microfluorometry, potassium salt, (2S)-2-amino-5-(carbamimidamido)pentanoic acid, ECTOL1, 14C-labeled, method used in an experiment, LC-MSMS, heavy, CG7835, CG42273, system, light, Lysine, Separated, Cytofluorometry, methods, anatomical systems, Flow Cytofluorometries, thallium (+1) salt, Lichtquant, experimental section, iones, Microfluorometries, beta-Trypsin, rubidium salt, labeling, Visible, calcium formate, methanoic acid, ions, OCTD, has or lacks parts of type, Flow, Flow Microfluorometries, photon, Cytometry, L-Arg, peptidos, cromium (+3) salt, TR2, 1110001I20Rik, sodium formate, PTHB1, Cytometries, Sortings, Controlled, Controlling, DYRK1, data, Ring chromosome 10 syndrome, nickel formate, 1-(14)C-labeled, 3H-labeled, Ufbp1, Cell Lines, strontium formate, Flow Cytometries, potassium formate, copper (+2) salt, L-Isomer, strontium salt, acetonitrile, CD258, DL Arginine Acetate, buffer, Cell, Peptide, mereological quality, polypeptide, LC/MS/MS, L-Isomer Arginine, Acetate, C18, cupric formate, chemical analysis, Hydrochloride, MFS1, L Arginine, Dyrk1, Vacuums, connected anatomical system, Flow Microfluorometry, aluminum formate, C20orf116, L-Lysine, ACETONITRILE, UFBP1, cyanomethane, WMS2, Radiations, AU020952, Separation, lithium formate, Ring chromosome type 10, HVEM-L, Separations, (2S)-2-amino-5-guanidinopentanoic acid, CC1, Photoradiation, Tripcellim, ethanenitrile, sample population, chromic formate, LTg, ME-IV, plan specification, Trypure, Photoradiations, Ion, cardinality, SSKS, Sorting, calcium salt, liquid chromatography tandem mass spectrometry, Peptid, assay, 6-diaminohexanoic acid, Enisyl, chromosome 10 ringAI132708, C13orf20, PCI domain-containing protein 1, HSN1E, Peptidomics, protein complex, Ufbp1, Proteins, number, DNMT, Gene, EA1, protein, function, gastric carcinoma cell line, MLR-3, MCMT, protein-containing complex, presence, dJ1187M17.3, BL-AC/P26, count in organism, protein polypeptide chains, AIM, native protein, natural protein, polypeptide chain, Protein, Gene Products, bA131P10.1, stomach carcinoma cell line, Fetal lung protein B5, protein aggregate, ENSMUSG00000074598, early activation antigen CD69, C20orf116, UFBP1, CXXC9, BM-002, early T-cell activation antigen p60, study, ADCADN, hFL-B5, Ufbp1., activation inducer molecule, 1810045K17Rik, ligand, AI326138, Gm10726, proteins, 2600009E05Rik, Protein Gene Products, Gene Proteins, stomach cancer cell line, leukocyte surface antigen Leu-23, RGD1309979, GP32/28, AI463323, eIF3m, C-type lectin domain family 2 member C, quantitative, 1110001I20Rik, CD69, CLEC2C, presence or absence in organismprojections, transcription factor, single-organism catabolic process, T-cell leukemia, cis-Dichlorodiammineplatinum(II), Randa, polyubiquitin, AGL4, (SP-4-2)-, dmTAF[[II]]230, protein polypeptide chains, Oxidative DNA, Oxidative, protein polyubiquitinylation, Dmel_CG13826, responsivity, Peyrone's chloride, Nitro-Oxidative Stresses, Pro Oxidant, gastric neoplasm, Cell Sortings, DNA Damage, TFIID TAF250, cel, catabolism, anabolism, cis-diamminedichloridoplatinum(II), cis-DDP, Protein Akr1c20, C2, Oxidative Cleavage, F10N7_150, Gm10726, proteins, RCH04A07, W, Briplatin, D17Mit170, allergic reaction, Oxygen, Oxidative Stresses, vertebra 2, medicine, (SP-4-2)-diamminedichloroplatinum, 26S proteasome, zinc ion regulated proximal promoter sequence-specific DNA binding, transcription from bacterial-type RNA polymerase promoter, TRANSCRIPTION FACTOR, RNA polymerase II distal enhancer sequence-specific binding, Cell Sorting, RNA polymerase II core promoter proximal region sequence-specific binding, C13orf20, dTAF[[II]]230, PCI domain-containing protein 1, anatomical protrusion, DNA-dependent transcription, GASC, familial, TAF200, C2 vertebra, Tl3, cis Platinum, Tl2, l(3)03921, NRF2A, shelf, Chemotherapy, CDDP, DNA Oxidative Damages, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, activation, Stomach Cancer, platine, breakdown, platino, Radical, Dichlorodiammineplatinum, [PtCl2(NH3)2], F14P3_4, AI326138, axis [C II], projection, Fluorescence-Activated Cell, 2600009E05Rik, ridge, 78Pt, Transcription factor, Nitro-Oxidative, Taf250, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, cisplatinum, DNA, Chemotherapy., metal ion regulated sequence-specific DNA binding, TAF230, Oxidative Damage, CG13826, transcription, advanced, anon-WO0153538.7, anon-WO0153538.6, lamellae, Antioxidative, number, stalk, biosynthesis, SEPALLATA 2, protein-containing complex, body system, process of organ, lamella, Fluorescence-Activated, Fluorescence-Activated Cell Sortings, Microfluorometry, zinc ion regulated core promoter proximal region sequence-specific DNA binding, E4TF1-60, sensitive, CG4566, bA131P10.1, system, Fetal lung protein B5, CNC, Cnc, Oxidative Damages, Cytofluorometry, BM-002, anatomical systems, Injury, dTAF[[II]]250, cis-Diamminedichloroplatinum, Flow Cytofluorometries, cell, 1810045K17Rik, ligand, axis (CII), NRF2, Nrf2, protein polyubiquitylation, Lederplatin, Expressions, culm, Platamine, synthesis, dTAF250, drugs, Platinum Black, Event Free Survival, RNA polymerase II proximal promoter sequence-specific DNA binding, Antioxidative Stresses, copper ion regulated proximal promoter sequence-specific DNA binding, Flow, Flow Microfluorometries, Cytometry, Expression, Pro-Oxidant, laminae, constitutitional genetic, Sortings, cellular transcription, cou, degradation, Cismaplat, anatomical process, Ufbp1, platinum, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, BG:DS00004.13, Flow Cytometries, Anti-oxidative Stresses, Cell, isoform CRA_c, Progression Free Survival, dTAF230, Lr, native protein, Oxidative Nitrative Stresses, Aldo-keto reductase, chemical analysis, proteomic analysis, TAF[[II]]250/230, RNA polymerase II transcription factor activity, CG43286, Flow Microfluorometry, UFBP1, organ process, cis-diamminedichloroplatinum, Progression-Free, Taf[[II]]250, cis-platin, hypersensitivity, Anti-oxidative Stress, cis-diamminedichloroplatinum(II), DNA Oxidative Damage, Neoplatin, cervical vertebra 2, cis Diamminedichloroplatinum, DNA-templated, CG4578, Platinum, cisplatine, sequence-specific DNA binding RNA polymerase II transcription factor activity, metal ion regulated core promoter proximal region sequence-specific binding, cisplatin, F10N7.150, cisplatino, AI194320, processes, 5134, AI463323, Biocisplatinum, Stress, Pro-Oxidants, Bra, E4TF1A, quantitative, hereditary, General activity, sequence-specific transcription regulatory region DNA binding, AI132708, DNA Oxidative, multicellular organismal catabolic process, Platinol, cis-Diamminedichloroplatinum(II), Activity, determination, second cervical vertebra, Nitrative Stress, protein, Platin, Damage, l(3)j5E7, axis vertebra, Pt, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, Nitro-Oxidative Stress, NFT2, Flow Microfluorimetry, Event-Free Survival, protein aggregate, Oxidative Injury, Peyrone's salt, multicellular organismal biosynthetic process, Oxidative Injuries, single-organism biosynthetic process, me75, hFL-B5, Gene Expressions, Fluorescence Activated Cell Sorting, Oxidative DNA Damages, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, T1, Anti-oxidative, genetic, DNA-dependent, Oxidative Stress Injuries, papilla, Cisplatin, AGAMOUS-like 4, Dmel_CG17894, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, associated, Flow Cytofluorometry, Fluorescence-Activated Cell Sorting, NSC-119875, Oxygen Radical, lamina, flanges, TAFII-250, TAF250/230, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, dJ1187M17.3, diamminedichloro-, Microfluorimetry, TAFII250, Oxidative and Nitrosative Stress, precocious, Oxygen Radicals, DmelCG43286, Ubiquitin, F14P3.4, proteasome, Cytofluorometries, shelves, Platinum Diamminodichloride, CG17603, TAF[[II]], early, organ system, Patient, spine, SR3-5, bacterial transcription, Platidiam, hypersensitivity reaction disease, Oxidative Stress Injury, inherited genetic, MCG9092, metal ion regulated proximal promoter sequence-specific DNA binding, Oxidative Stress, d230, Antioxidative Stress, Gene, dTAFII250, Reactive Oxygen Intermediates, EfW1, presence, protrusion, Stresses, Oxidative Nitrative, polypeptide chain, dmTAF1, Stress Injury, Taf230, Survival, Oxidative Cleavages, Low, sequence-specific DNA binding, ENSMUSG00000074598, sensitivity, Reactive, TAF250, reactivity, Taf200, formation, Microfluorometries, cis-Platinum, Taf1p, ridges, Event-Free, Oxygen Species, gastric cancer, Anti oxidative Stress, Active Oxygen Species, Oxidative DNA Damage, Clients, hypersensitivity reaction, 1110001I20Rik, TAF, Active Oxygen, Cytometries, TAF[[II]]250, Pro Oxidants, protein complex, axis, l(3)84Ab, Client, Gastric cancer, transcription factor activity, count in organism, Oxidative Nitrative Stress, BcDNA:RE05559, cervical axis, natural protein, p230, Protein, (SP-4-2)-diamminedichloridoplatinum, TFIID, Active, connected anatomical system, C20orf116, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, flange, CG17894, cis-diammineplatinum(II) dichloride, poly-, cis-[PtCl2(NH3)2], hypersensitive, TAF[[II]]230, cis-dichlorodiammineplatinum(II), Diamminodichloride, CNC_DROME, TAF[II]250, Cleavage, copper ion regulated core promoter proximal region sequence-specific binding, process, DmelCG17603, eIF3m, Sorting, Platino, processus, assay, response, Nitro Oxidative Stress, Platinex, TAF1, presence or absence in organismBru, IPP2A2, Raw, Lysine Hydrochloride, L-arginine, DL-Arginine Acetate, AI326280, Aminosaeure, Amino acid, Monohydrate, Visible Light, protein, 5730420M11Rik, peptide, Polypeptides, L-Isomer Methionine, protein polypeptide chains, Lysine Acetate, Methionine, peptido, hnu, 2, Arg, protein aggregate, foton, WMS, L Lysine, Svc, SET, (S)-2-amino-5-guanidinopentanoic acid, F, amino acids, peptides, reference sample, TAF-I, K, proportionality to, M, phosphatase 2A inhibitor I2PP2A, (S)-2-Amino-5-guanidinovaleric acid, human protein, R, epsilon-diaminocaproic acid, Arginine, Monohydrate DL-Arginine Acetate, proteins, DmelCG4299, set, IGAAD, LYS, Lys, DmelCG10574, ppm, Lysin, Half Cystine, GPHYSD2, Racemethionine, SGS, Lzm, gamma, L-Arginin, phapii, Lzp, Radiation, Zinc Cysteinate, Aminokarbonsaeure, T5E21.12, 2-Amino-4-(methylthio)butyric acid, Arginine Hydrochloride, StF-IT-1, arginine, Light, alpha-amino carboxylic acids, ACMICD, LIGHT, lysine, Methionin, methionine, Lzm-s1, Hmet, T5E21_12, parent ion, L-Arginine, Visible Radiations, Amino Acid, Acid, Visible Radiation, HLA-DR-associated protein II, HVEML, Amino acids, DI-2, I-2Dm, proportionality, rate, L-Cysteine, MASS, CG4299, alpha, inhibitor of granzyme A-activated DNase, beta Trypsin, experimental procedures, I-2PP1, Lysm, light quantum, TAF-IBETA, precursor ion, TAF-Ibeta, Acids, Polypeptide, i2pp2a, Liquimeth, Cysteine Hydrochloride, 2-amino-4-(methylsulfanyl)butanoic acid, experimental, False, Aminocarbonsaeure, L Isomer, FBN, high weight, precursor, alpha-amino acid, protein-containing complex, PHAPII, L-(+)-arginine, Ly113, template-activating factor I, (2S)-2-amino-5-(carbamimidamido)pentanoic acid, polypeptide chain, ECTOL1, heavy, L-Methionine, Del(8)44H, Lysine, light, Half-Cystine, Pedameth, proportion, methods, L Cysteine, Lichtquant, experimental section, ipp2a2, beta-Trypsin, 2pp2a, Visible, CG10574, OCTD, 2PP2A, 2-amino-4-(methylthio)butanoic acid, 10^[-6], taf-ibeta, dSET, dSet, photon, L-Arg, peptidos, TR2, Controlled., metionina, Lyzs, data, Controlling, DL-Methionine, protein complex, igaad, alpha-amino acids, backward, L-Isomer, DL Arginine Acetate, CD258, Peptide, polypeptide, native protein, natural protein, I-2PP2A, L-Isomer Arginine, Acetate, Dm I-2, Protein, I2PP2A, Hydrochloride, MFS1, L Arginine, L-Lysine, Data Base, WMS2, Met, Radiations, Col4a-1, HVEM-L, (2S)-2-amino-5-guanidinopentanoic acid, Photoradiation, Tripcellim, Amino, LTg, Trypure, dSET/TAF-Ibeta, 2610030F17Rik, Photoradiations, SSKS, alpha-amino-gamma-methylmercaptobutyric acid, quotient, Peptid, AA407739, 6-diaminohexanoic acid, Enisyl, reversedProtein Gene Products, Gene Proteins, Controlling, reference sample, Protein, Ufbp1, Gene Products, Proteins, AI326138, Cell., Gene, proteins, 1110001I20Rik, C20orf116, 2600009E05Rik, UFBP1, dJ1187M17.3, Controlled, MGC803falseSILAC quantification of proteins in the control and UFBP1-expressing MGC803 cellsIn this project, we used SILAC quantitative proteomics to identify proteins that are differentially regulated by UFM1-binding and PCI domain-containing protein 1 (UFBP1, also called DDRGK domain-containing protein 1 or DDRGK1) in a gastric cancer cell line. The aim of this study is to discover the proteins that are responsible for the biological function of UFBP1.2021-02-152019-04-23PXD01359028065516460991804982953585111454224942024527872842188090368093270429330845811363336351524260119834300662758385NCBITaxon:128068888561896111430118726355557114526236493461364031952065126957366649274670738538836411354615821582310665688954762721123294422291180992323122586365458333460792519365914320597129386558195851149315242592637371250232NCBITaxon:127211195774818661488584310430024513586722175543740816918045426683429531945305861266835149822505814963674452158554528113208147956410039237514636942049433208964208963445974137979138712728384400667453293529367156759388036478434716052165994048321659721659545501315282693042165915627161696833218321729204277944762376454317203094980197525556978159509439843948840479NCBITaxon:21575253381053551531051067439899037122857759515205968762262311210884555588845587077918178081736309431794571698936160488409859NCBITaxon:371621047826794015059071483643204210613204913816936303555432046632633311994565111024535110254111032174592161292642012886764100264203694569410167634102107612773515248671316582392547279334281461358430141729876091746334577444916754760489321287356756840301350621917265181301941119294393671104113102393202555986325827883965910475934749516049016529983552566317585439801102432608663102459808757417555183201964471452680457966313670190832331156114155355104219325552113834397634981812359871069955529NCBITaxon:4751850298335476113615741465477180066556484337677299767982510216998238976484074137351155711089725482444474153NCBITaxon:505578996984410003646534933414714749354801946751299114359741276473137086326547876569835322159983819201110677577271034819852348780265554479464098609958615718717668599191572951372211711012929210768783497419860202809148840633424923423515233598598865597464998703920211107271139943311363527215987419085037117082711708284182350241451139692931902197822474636046147123749402375939723287581722532566489050794526591972211915853101774208588596NCBITaxon:615750521028307989515586411051989972375059576131720002634196102641088878176179212190659407121906772275885815689964975151950582873757244NCBITaxon:22023717NCBITaxon:17036122019998570506050617476792745062724915961533355418400524643213686150596487844612286662941786661347515363291018194301086731018510184NCBITaxon:176357303963558633149286287670580132165085271848461051865368595105135553482260710185687294296110091416333NCBITaxon:619184023210007348824123363630710470058127187878645159812894555663577553470951451225786425011260704NCBITaxon:133562626070526070765845740541636774831672331502951803561007133705654195NCBITaxon:305550445791399878168119011928752829126806315327341478281055524NCBITaxon:9606135858469008111167815022830519302836728593008529005NCBITaxon:56938268830640179469915101184994352587591315159014482428381113944225844160419370475927237086042328167728503515819901195195326738637013702492405360883858162248818615622774881756261583558371533376057267119350199808611935021678791085791371137127240719065037147591726298157125627457572709030903113335503728142433747924910078711082NCBITaxon:96157243359463318586260799141262200298492670NCBITaxon:1263719281249212528303856838569591169266100658137352375612750454266625515197881356638375064422336009412934979054632245352472140125751511649908515151438992374537474454464152432359376059202228870515805955365550881579327160293851578114978633892894535936479437NCBITaxon:685441275422511715901090134167387158955210129661030612967158515845158493765939343271591002263767338899046142345128768959119833296646704146479293657616428034116022164133159928038280353690910891768814989462181214108034817939228788929502711313329528282944568617493468191126526997227321890382107601247190400727391521597369978281143114020641198108713554774365814245072295337969820423212422312916321803347087145372091645733215358157546992877702020603281143193382935508332912922114864374572526499972830357390871830872672710379085507176299707678926837041557372921405329412816078395639243142626548982273775611559620732208659588273555169360533079725518113549143213715974953502611595561432138406072587814144715094933491949634016143347280059360463164610466038151845474485001561972356709747777195124017484185560133575554792042741580536076994688103762014887100594120037582890931643542826885007123038399001389464940838283538283494144242322197857742171598921409212205853939621157781154756929793244704749906604162103591905158114452749907312987779913112273170187546991103589915NCBITaxon:372961036098334187104911307981336795316407299467103401578658264530333733063830113064139925306423636505911696547885469811211620343176526161033728612994044586207340115357111952965261174553111471613386541313166343055213231201798360253237312761255232993512993850573163176426197940115134584457726787286232193341513461862086211607913922279835168119612552281495393223231167617573122502409108077213487999948861856962814084341033517787996321849620994887321392488209711533820961153392095330439375569927612686266447NCBITaxon:2697049339862436941103121958832050920290610130685999758644458828643249952529724642606674115834321271789427863789426147740525673786391233435514199834931799868655405534352914865451471245466889251042986357124679151641358257632884019373345087718238039129039129529719748693179789110286889995133731054878665220341712516321389850762554701193806222523256318865713034431121308516558658862238NCBITaxon:44664946454636493NCBITaxon:1107114280062421241873032883564913743751415167845881087439145943854331116781077854314408043933452703134NCBITaxon:110829534623226939773821899318226900351283871459531168853399669527869595418694200308954486972443662066563954530055862335518042643086922003022848124264281504751499105102991029847147241445238626290319541832902512089557497431170089306218644193061575584131016538235230805655772238235939946360106601623601043994839947179748254440423761021279056881337751823635572116229629817677187093128112147861050238245185579669465431863205182406672829744173944932351245012885318388919867948853191113782559103716473516601261557228331349390NCBITaxon:101142845031631297801985738595239693934323795602608712134639598427529595266437334323223544314275242243081433619593133456582609432749592560291699631122233605201216979525283869728529046436801218707334747279010132342956031278161119219408188297603818124988563122034018843973312744141639107243139855858885872520493692955464803834369122432640817212419355955217493991281614055920425465519458228860918382727592127442340817019332982972275985955712744201274426235455552857120729432841399616601876254829647187614378681490247480119915095648188229725431761012148015412045514816723847384818829451906951503034166336036165984048498505693101510528354528353528352693735294512637534052066987418649729722405212585941097677451804812310061911079NCBITaxon:81347800941269432271027943447256848479291146387033384991722617561094616143512032581205136521850523525944732054942334937347386452700044664499175918915825803880433303766862448046120678112202604991080612461962902224619732063752299186705118670575090681219387911879471868023980071868015723072154514683574125814081861175715084769183885803290311608611388869152167781147276721271103683997844597247512951398704351262469692812397713333995226126103430481381781821981318826821080228156100547624112292657163295755350192222197911119686669381979121247124614854513283882813951080230294911040145954161934139036312041622110665890326446071111702272104117061274432108595621028316941283166694369455972994951410658882711411316187901010584133087947844751741508081705394206542317447127070996415329242814111946698411454587932769537932920101981264690410661550537145755139251923239232014081576401316973160621213392830312383911283128224260512803395228110108328252535315285128112853353153498257419481478753623142410135674295105242619145481NCBInr 20130519115062129459706473129282996339413469597538631211705193516265390021343980313098006978103690122020718853225660173654856615664162425103695118702348334824688221187056386321219690226186129925016468606885656512623217219565810102865606165606442838656717136041602358700349415048022160100298823578613252911187002157641809699756655666452467134887937965223926291613633666336721751117645406560103789059232146429314344870933114767468342808NCBITaxon:4055923590932264652365262126337498217870749821617583163472565690569156925693872249821165448720569734507699300166690510894491089448NCBITaxon:2759661410NCBITaxon:4932653587155689520710894441000589655087322875351352481263730069303926659581143988725121177687268723872487291178665498727134976724223130554417725784541581419198822445446NCBITaxon:120661174673104105909882497473446899601677674468587529600NCBITaxon:581111822637082484119165578457845848402119443955307158059656636164034624136810893161001428099612NCBITaxon:548681NCBITaxon:5820546414394503307757975522836373791966272905815260096059606875944670875622708626659649604323259570509335483309024444729829387834810626756726756643061512793654319474483854492394704721122984119152319170161089615483139691692878275736221862840782112931457075298352061892577712850248052701262834854189854871427867158779627300251563041126736125994952869646964396444417684911462472746128746011345069638526385263996563024097639245264110909467469416348112386935174635231NCBITaxon:56599527606576784119562330591736582257313269796990346134434636064404213451124951533043479096109760616194032257329246200966927294227294351338968583554767894314696891280175576009681118696932011167807811869414361833303811869817194759321718266265118691443906NCBITaxon:10298NCBITaxon:7771257118387271566338404330618596948364190802703847664568703257310568708618218678661833568851350NCBITaxon:7803906451515NCBITaxon:15029903164515169431297029820762115861218451210725730919307910944609137494400209285201319747078529507619212638546146663915383379NCBITaxon:10239NCBITaxon:714173613095705470448300641751585923890037159084128108406972282334575412NCBITaxon:1024528104NCBITaxon:10243392017481097797067135622583313618550992428670652779658334240906200492910263494951034809NCBITaxon:6312406741307670703766191009408707487613810804329032176023993592672947474376032768917812126719285321773846451772177437272557104905615348119922512153239576769014NCBITaxon:722715697472664709131704725852092026971303276978596285963411476130913081307126387121203533264837315321469338323411479398158370131469030713134937608333413118333357047111497039655397283332101841833312146874550451080187492002627241530091076620637373505411490280148517501303713444143983NCBITaxon:115523988139927515622435623593117928662623168390614404211945995677716032932853422NCBITaxon:5759345089998103365786185336583517431275457428316273121536024271831627713528086913519948875058NCBITaxon:10407316275242715502779167971820795413496600271306274154967550278029159541264432392915866002557021272113558867551234916129658793094520312410515192843435815398580156269811825902471491331678456099069027256027256122142725633544228410133562634166392867572279017904NCBITaxon:57965374575740574111233523562378548355522221045775126216131091575596915429310136413962242139213901857323271489622392307411640278128810489616010675744580240251229683352944139366581403665762576344322066724005402965433040162NCBITaxon:1170914082742823177298184318344322622469765755499229575757592845935561822845907066714367334442403531849223112105421152763910969762261111795752981882633184319229438152764098666134312416265281044214438679626782531751327804267608565050176946996NCBITaxon:131317125079533894279558801198732519795962141140822414082232645614219710141228518676318543152076773767774219253066129454458057041744726724042649329NCBITaxon:1773208893208895207559101176645531523166330200436745101161328426656722658721392696358895423019531882915819665588391943665341123368131359044982249115505543826413103379986669400688456668421576711435353115104298315468911129859356253232273075660469136165105612149165238976336316812149159704126308213494091328881885513613NCBITaxon:1865381889083264241916073NCBITaxon:186536621017366041185650315750393765573111623427306855743680863772163157491529526689535862048867NCBITaxon:138862962305959755284003197291761608994291703002731052311009110090405410092646532243232760568340621786061064539477816198215973930627807574998024199474275775804227779195524913136623940731300146407662532041044111170869374515479221198561198572992014625944072NCBITaxon:5920165636713926767162481450134408440877111977810740162699412791314781967949679540814082380394528135340381659668872292612351786162432312432301359923561271087671797854096409797882103529305243277627912924911557484090978040924093447135120253163761629838633178313625152002238865856944155401813397174168709796628773239669202979362899315634301922344713763433872227954866343629916628233456433169173623125924858611093667421386388420493211354724847947314473116968111290255161398128481317333672214407721316931555778122152250255183610036NCBITaxon:11266NCBITaxon:439488113132318679448485145494137499010041715916922596345910659213334991065901249615334542NCBITaxon:6622432754427124327421340112390110049716571678496410289688333295491249668425282652158109434326521577160197127908571622562237NCBITaxon:103572702NCBITaxon:1035811948816543605076011415784435590NCBITaxon:103591707212726348008185934783413223438022113445826276261890364196947139501982528634955983964271191364527262127262327262411607191177189123899312141024605231218948403211821314269704940324803113180803049031417981305212483431114778749093238481437113153688918413684174940804980405788225931943427149182837342561364911543734131567131641631594030210097146297728235256750601895192952492914505111524964922274849249673146051951876626020115334858002153818625622691232801360327561423142227511903041434011911022145052049321017552759142849342664179906914174609111020594082511211143919042824582762167539131463977851866139077527142735071061981353008559292148691719825214511577281414448340732036962232834959NCBITaxon:1167612296211634342375451367830308678495237109467989558015802362733219317