{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Txt":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/peptides.txt","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/proteinGroups.txt"],"Raw":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/WCX_1704055_C2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/WCX_1704055_L24_2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/WCX_1704055_L24_1.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/WCX_1704055_C1.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/WCX_1704055_L2_2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/WCX_1704055_L2_3.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/WCX_1704055_L2_1.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/WCX_1704055_L24_3.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014259/WCX_1704055_C3.raw"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"labhead_mail":["karencx0465@163.com"],"submitter":["Chunxia Wang"],"technology_type":["Mass Spectrometry","Shotgun proteomics"],"software":["Not available"],"submitter_keywords":["Protein profile","Lc-ms/ms","Serum","Mice","Lps"],"full_dataset_link":["http://www.ebi.ac.uk/pride/archive/projects/PXD014259"],"tissue":["Blood Serum"],"sample_protocol":["To mimic sepsis, mice were treated with 10 mg/kg LPS (E. coli 0111: B4) to construct mice model. Male C57BL/6J mice (8-week old) were purchased from the Shanghai SLRC Laboratory Animals Co. Ltd. (Shanghai, China). Mice were maintained on standard laboratory mice chow in a 12-h light-dark cycle and access to dry pellets and sterile water ad libitum. The care and use of the mice were conducted according to a protocol that was approved by the Ethics Committee of the Shanghai Children’s Hospital (Shanghai, China). A total of 30 male C57BL/6J mice were randomly divided into three groups (n = 9 per group) including control group, LPS treatment for 2h group (LPS 2h), LPS treatment for 24 group (LPS 24h). The mice were treated with vehicle (0.9% saline) (control group, n = 9) or LPS (10 mg/kg) (LPS group, n = 9) by intraperitoneal injection. The mice were anesthetized using pentobarbital (0.2mg/kg) by intraperitoneal injection at 2h or 24 h after LPS administration. Blood was collected from heart and centrifuged at 3000 rpm for 10 minutes to separate serum. The serum samples were kept at -80oC for further analysis. In order to increase the precision and accuracy of the data in proteomics study, equal amount of 3 different samples were mixed to produce a sample pool. After that three sample pools were generated (control group, LSP 2 h group, LPS 24 h group, n = 3 per group). The serum proteomic analysis was performed by Kangcheng. We used 200 μl serum to remove high-abundance serum proteins such as albumin, IgG, and haptoglobin using the ProteoMiner™ Protein Enrichment Small-Capacity Kit (Bio-rad, cat: 1633006, USA). Then, the proteins were concentrated and desalted. A total of 2 μg protein from each group was used to LC-MS/MS analysis. The peptides were separated by nano-UPLC system (EASY-nLC1200, ThermoFinnigan, USA) and were detected by using online mass spectrometer (MS) (Q-Exactive,Thermo Finnigan,USA). Samples were subsequently loaded onto the Reprosil-Pur 120 C18-AQ column (1.9 μm, 0.1×300 mm, Dr. Maisch). The components produced by chromatography were subjected to Q-Exactive MS (Thermo Finnigan, USA) analysis. The ratio of the peak area of reporter reflected the relative abundance of peptide and protein."],"repository":["Pride"],"quantification_method":["Not available"],"modification":["No PTMs are included in the dataset"],"data_protocol":["The MaxQuant (version 1.5.6.0) was used to identify peptides. The MS/MS data were searched against the mouse International Protein Index database (UNIPROT_MOUSE_2016_09) with parameter settings as described previously. And quantity of protein was detected by using the method of label-free (LFQ). In order to reduce false positive results, a strict cutoff for protein identification was applied with the unused ProtScore > 1.3 and at least one peptide with 99% confidence limit. The ratio of protein expression between the two groups (> 2.0) and adjust p-value < 0.05 were considered significant. The cellular component, molecular function, and biological process were analyzed through Gene Ontology database (http://geneontology.org/). The protein–protein network was analyzed by STRING software (http://string-db.org/)."],"omics_type":["Proteomics"],"labhead":["Chunxia Wang"],"instrument_platform":["Q Exactive"],"labhead_affiliation":["Shanghai Children's Hospital"],"submission_type":["PARTIAL"],"species":["Mus Musculus (mouse)"],"submitter_mail":["karencx0465@163.com"],"publication":["32322166 Wang C, Cui Y, Miao H, Xiong X, Dou J, Shao L, Tang X, Zhang Y. Apolipoprotein A-V Is a Novel Diagnostic and Prognostic Predictor in Pediatric Patients with Sepsis: A Prospective Pilot Study in PICU. Mediators Inflamm. 2020 2020:8052954 10.1155/2020/8052954"],"submitter_affiliation":["Shanghai Children's Hospital"],"submitter_country":["China"],"pubmed_abstract":["