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ide/data/archive/2020/06/PXD019789/FRL8770A15MA3__015-1FRL8770A15MA3-40-306735.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019789/FRL7876_allMSMS.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019789/FRL8770_allMSMS.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019789/F062874.datftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019789/F064685.datprimaryOK200jp.schnitzler@helmholtz-muenchen.deAnn-Christine KönigMass SpectrometryShotgun proteomicsDisease FreeNot availablepoplarisoprenereactive oxygen speciesrosvolatile organic compounds.Populus x canescens; ros; voc; isoprene; isoprene promoter; lateral roots; poplar; reactive oxygen species; roots; volatile organic compounds.voclateral rootsrootsisoprene promoterPopulus x canescenshttp://www.ebi.ac.uk/pride/archive/projects/PXD019789Plant CellRootFor proteomics analysis 50 mg of frozen, homogenized, grinded root tissue was dissolved in 500 μl of 1x Laemmli buffer (240 mM Tris-HCl, pH 6.8; 8% w/v SDS, 40% v/v glycerol, 0.08% w/v bromphenolblue, 20% v/v β-mercaptoethanol) and incubated at 95 °C for 5 min. After centrifugation (14,000 x g for 10 min) supernatant was separated and protein content was determined using the Bradford assay (Bradford, 1976). Aliquots that contained at least 10 μg of protein in 50 μl of solution were prepared for further analysis for liquid chromatography – tandem mass spectrometry (LC-MS/MS). Eight ± two independent, biological replicates were analyzed for IE and NE plants. Each 10 μg of root extract were digested with a modified Filter-Aided Sample Prep (FASP) procedure (Wiśniewski, Zougman, Nagaraj & Mann, 2009; Grosche et al., 2015). Briefly, the proteins were diluted, reduced and alkylated (using dithiothreitol and indole-3-acetic acid). Then the proteins were centrifuged through a 30-kD cutoff filter device (PALL), and washedthree times (once with UA buffer (8 M urea in 0.1 M Tris-HCl, pH8.5) and twice with 50 mM ammoniumbicarbonate). The proteins were digested for 2 h at room temperature using 1 mg of Lys-C (WakoChemicals) and for 16h at 37°C using 2mg of trypsin (Promega). The further details have been described in Vanzo et al. (2016).PrideNo PTMs are included in the datasetLabel freeFor mass spectrometric analysis the samples were thawed and centrifuged (14,000 x g, 5 min, 4°C). LC-MS/MS analysis was performed as described previously on Ultimate 3,000 nano RSLC coupled to a LTQ OrbitrapXL (Thermo Fischer Scientific, Hauck et al., 2010; Merl, Ueffing, Hauck & von Toerne, 2012). Every sample was automatically injected and loaded onto the C18 trap column. After 5 min, the peptides were eluted and separated on the analytical column (75 µm i.d. x 25 cm, Acclaim PepMap100 C18, 3 µm, 100A, Dionex) by a linear 135min acetonitrile gradient from 4 to 30% at 300 nl min-1 flow rate. From the MS pre-scan, the 10 most abundant peptide ions were fragmented by collision induced dissociation in the linear ion trap if they showed an intensity of at least 200 counts and if they were at least doubly charged. During fragmentation a high-resolution (60,000 full-width half maximum) MS spectrum was acquired in the Orbitrap with a mass range from 300 to 1500 Da and a dynamic exclusion of 60 sec. The acquired spectra were loaded to the Progenesis QI for proteomics software (version 2.0, Nonlinear Dynamics, part of Waters) for label free quantification and analyzed as described previously (Hauck et al., 2010; Merl et al., 2012). Features with only one charge or more than eight charges were excluded. The raw abundances of the remaining features were normalized to allow correction for factors resulting from experimental variation. All MS/MS spectra were exported as Mascot generic file (mgf) and used for peptide identification with Mascot (version2.5.1) in the Populus trichocarpa protein database (30197159 residues, 73016 sequences). Search parameters were: 10 ppm peptide mass and 0.6 Da MS/MS tolerance, one missed cleavage allowed, carbamidomethylation of cysteins was set as fixed modification, methionine oxidation and asparagine or glutamine deamidation were allowed as variable modifications. A Mascot-integrated decoy database search calculated a false discovery rate of 1.36% when searches were performed with a mascot percolator score cut-off of 13 and a significance threshold P=0.05. Peptide assignments were re-imported into the Progenesis QI software. After summing up the abundances of all unique peptides allocated to each protein, the identification and quantification results were exported. The annotation and functional classification were achieved by using several databases. Firstly, the list of primary IDs identified by Mascot was exported into Phytozome v12.1.6 (Goodstein et al., 2012) using the Biomart tool. The Arabidopsis reference genome was chosen as a template and poplar genes were blast searched against potential matching Arabidopsis genes, resulting in a list of Arabidopsis orthologs and GO terms. Subsequently, the list of gene ID was exported in PopGenIE (The Populus Genome Integrative Explorer) to confirm previously predicted functions (Sundell et al., 2015). The PPDB (The Plant Proteome Database) was used to allocate in MapMan BIN categories (Thimm et al., 2004; Usadel et al., 2009).ProteomicsProf. Dr. Jörg-Peter SchnitzlerLTQ OrbitrapHelmholtz Zentrum München, Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Ingolstädter Landstr. 1, 85764 Neuherberg, GermanyPARTIALPopulus Trichocarpa (western Balsam Poplar) (populus Balsamifera Subsp. Trichocarpa)ann-christine.koenig@helmholtz-muenchen.de32495947 van Doorn MM, Merl-Pham J, Ghirardo A, Fink S, Polle A, Schnitzler JP, Rosenkranz M. Root isoprene formation alters lateral root development. Plant Cell Environ. 2020 10.1111/pce.13814HMGU Core Facility ProteomicsGermanyIsoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analysing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non-emitting poplar roots revealed an accumulation of O<sub>2</sub><sup>-</sup> in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signalling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S-lipoxygenase, in isoprene non-emitting poplar roots. Together our results indicate for isoprene a ROS-related function, eventually co-regulating the plant-internal signalling network and development processes in root tissue.Root isoprene formation alters lateral root development.Miloradovic van Doorn Maja M, Merl-Pham Juliane J, Ghirardo Andrea A, Fink Siegfried S, Polle Andrea A, Schnitzler Jörg-Peter JP, Rosenkranz Maaria Mliquid chromatography tandem mass spectroscopy, determination, Tandem, PreP, classic hairy cell leukaemia, SDH, Hydrogenchlorid, protein, 2-(indol-3-yl)ethanoic acid, Basodexan, GRO:0005338, AA410010, composed of, Mass Spectrometry Mass Spectrometry, extracted material, SDS, Ethanol, protein polypeptide chains, hydrogen chloride, exact), Glycerol, IAA, Pro-Mega., Pop, HCL-C, 1, Urea, 2, Min, protein aggregate, present in fewer numbers in organism, DmelCG42273, (R*, 3-Propanetriol, portion of tissue, Oelsuess, Divorced, LCMSMS, E927b, Tissue, hypoplasia, 2 Mercaptoethanol, min, mAPC, composition, Divorces, proteins, 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complex, F10B6.15, CG7826, LC-MS-MS, Intervention or Procedure, Buffer, Karbamid, Glycerine, glycyl alcohol, Propanetriol, polypeptide chain, reduced, subnumerary, Trihydroxypropane, LC-MSMS, Harnstoff, CG7835, Gene Products, CG42273, tiny, Carmol, Separated, cloruro de hidrogeno, propane-1, Hydrogen chloride, interventionDescription, MS2, Glycerin, beta-Trypsin, 4733401P19Rik, Interventional, Indoleacetic acid, decreased number, study assay, Solution, hairy cell leukemia, glycerolum, (indol-3-yl)acetic acid, small, measuring, Intervention Strategies, 1H-indol-3-ylacetic acid, radices (exact, DYRK1, raíz (Spanish, protein complex, Glyzerin, Proteins, 3-triol, D10Wsu136e, Clelands Reagent, chloridohydrogen, compositionality, buffer, all, [HCl], LC/MS/MS, native protein, Temperatures, natural protein, carbonyldiamide, chemical analysis, Protein, Hydrochloride, Dyrk1, tandem MS, Intervention, Cleland's, AU020952, 3-Trihydroxypropane, Separation, underdeveloped, uree, Separations, urea, CC1, Ntup1, 3-Indolylessigsaeure, Tripcellim, 2310012C15Rik, SWDS, Mass Spectrometry-Mass Spectrometry, CGI-97, sample population, UREA, ME-IV, Protein Gene Products, Cleland's Reagent, Gene Proteins, Trypure, structure, 2-mercapto-, liquid chromatography tandem mass spectrometry, assay, PE, AI450383, hydrochloric acidall, synthesis, 3-butadiene, radices (exact, radix (exact), single-organism biosynthetic process, 2-methyl-1, exact), multicellular organismal biosynthetic process., raíz (Spanish, climbing root (narrow), formation, anabolism, plural), aerial root (narrow), 根 (Japanese, root, biosynthesis, GRO:0005338CPD photolyase activity, western balsam poplar, MeCN, Materials, PhrB photolyase activity, ion, Raw, Black, A., Populus trichocarpa Torr. et A.Gray, Cardaminopsis, 5730420M11Rik, Polypeptides, protein polypeptide chains, L-glutamine, Populus fremontii, Drug Tolerance, hierarchy, systematics, CH3-C#N, A. thalianas, B1, SEC, 2, L-2-aminoglutaramic acid, 3, NUP96, Software Engineering, outer pigmented layer of retina, epithelium, WMS, C79691, DL-Asparagine, SET, Divorced, Theory, asparagina, F, asparagine, Genomes, M, N, phosphatase 2A inhibitor I2PP2A, L-Asparagine, sec, Q, Kitl, Divorces, proteins, Populus balsamiferas, Mast cell growth factor, Cottonwoods, Mouse-ear, SUPPRESSOR OF AUXIN RESISTANCE 3, AI047805, Lccp, DmelCG4299, Black Poplar, set, pigment epithelium of retina, column, hemocytoblast, sample, D1, n, blast, pigmented retina epithelium, CT, drug tolerance, SGS, LC-MS2, Chaos, immune system tolerance, Ct, Systematics, somatomedin, IBP1, not genetically inherited, predicted, Software Tools, ACMICD, Nonlinear, Computer Applications, Black Poplars, Dm1, Computer Applications Software, dipyrimidine photolyase (photosensitive), Methionin, methionine, Hmet, Nonlinear Models, Non linear Models, poplar, Software Applications, poplars, HLA-DR-associated protein II, DI-2, poplar trees, Source Software, I-2Dm, Stem cell factor, proportionality, rate, 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Model, metionina, PTHB1, 1-(14)C-labeled, True, hematopoietic growth factor KL, Populus balsamifera subsp. trichocarpa (Torr. et A.Gray) Brayshaw, Arabidopsis thaliana, split, acetonitrile, L-Isomer, BCM1, Tool, polypeptide, native protein, Immune Tolerance, I-2PP2A, C18, chemical analysis, Dm I-2, hemorrhaged, Glutamic acid 5-amide, MFS1, BLAST1, Met, T6K12.14, AU020952, torn, RPE, Col4a-1, Glutamic acid amide, Non-linear Dynamic, Separations, Populus L., thaliana, Dynamics, ethanenitrile, retinal pigment epithelium, 4-diamino-4-oxobutanoic acid, ME-IV, p. pigmentosa retinae, Computer Programs, Applications Softwares, MGF, Mgf, quotient, liquid chromatography tandem mass spectrometry, deoxyribonucleate pyrimidine dimer lyase (photosensitive), Immunological Tolerance, liquid chromatography tandem mass spectroscopy, Bru, IPP2A2, True Poplar, NCMe, determination, Pflanze, Somatomedin-C, cleavage, protein, pigmented epithelium, contrasted, BLAST, L-(+)-glutamine, viridiplantae, peptide, Classifications, Cut, L-Isomer Methionine, hierarchies, Arabidopsis thalianas, Methionine, L-Glutaminsaeure-5-amid, peptido, ClvPrd, l(1)7Ba, l(1)7Bb, Min, protein aggregate, Computer Program, DmelCG42273, Svc, Chaos Theories, LCMSMS, peptides, TAF-I, Open, D-Glutamine, Mouse-ear Cress, pigmented retina, somatomedin-C, min, Computer Programs and Programming, SF, mAPC, SCAN, free, ASN, DNA cyclobutane dipyrimidine photolyase activity, Asn, CG11387, SLAMF2, IGAAD, Nonlinear Dynamic, Populus nigra, DmelCG10574, IGF1, ppm, Mechano growth factor, Soluble KIT ligand, Theories, GPHYSD2, Sl, Arabidopses, Racemethionine, ratio, phapii, PRE, IGF-I, steel factor, MEM-102, mCD48, T5E21.12, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, LC-MS/MS, 2-Amino-4-(methylthio)butyric acid, Dmel_CG7826, StF-IT-1, A. thaliana, Populus fremontius, Data Base., pigmented retinal epithelium, Ids, Populus, fragmented, Source Softwares, results, sKITLG, Programs, Program, Taxonomies, retinal pigment, Ionen, black cottonwood, mechano growth factor, Computer Applications Softwares, Immunologic Tolerance, Genetic Materials, Softwares, BcDNA:GH10590, Igf-1, retinal pigment layer, Dmel_CG7835, Genetic Material, L Glutamine, T5E21_12, Mnb, MNB, SHEP7, CHARGES, mast cell growth factor, retinal pigmented epithelium, cracked, Plant, hCD48, MASS, CG4299, AW124434, balsamiferas, L-glutamic acid gamma-amide, Kitlg, phr A photolyase activity, Arabidopsis, KL-1, DNA-photoreactivating enzyme, Tolerance, KITLG, Cistron, Polypeptide, Cottonwood, i2pp2a, Balsam, Proteomes, MPS2, experimental, AW549739, taxonomy, 5-diamino-5-oxopentanoic acid, L Isomer, FBN, Gene, FPH2, Computer, Poplar, photoreactivating enzyme activity, (2S)-2-amino-4-carbamoylbutanoic acid, PHAPII, LC-MS-MS, Blasts, True Poplars, polypeptide chain, template-activating factor I, ECTOL1, Non-linear Dynamics, L-Methionine, stratum pigmentosa retinae, Del(8)44H, nigras, Separated, Models, Pedameth, Taxonomy, MOS3, Non linear Dynamics, methods, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, Genetic, PRECOCIOUS, iones, experimental section, Selb, ipp2a2, OCTD, Asparagin, 10^[-6], DmelCG11387, taf-ibeta, Populus balsamifera subsp. trichocarpa, L-Glutamine, hematocytoblast, Mouse ear, Model, F23A5_3, Applications Software, Open Source, DYRK1, Computer Software, 3H-labeled, DL-Methionine, protein complex, Balsam Poplars, igaad, total expressed protein, Non-linear Models, L-2-Aminoglutaramic acid, Cistrons, Peptide, Poplars, stratum pigmentosum (retina), LC/MS/MS, MODIFIER OF SNC1, Software Tool, secret agent, natural protein, Protein, deoxyribonucleic photolyase activity, I2PP2A, Dyrk1, L-Glutamin, Software, ACETONITRILE, WMS2, cyanomethane, Data Base, Populus balsamifera, Separation, 10^[-9], Non-linear, photolyase activity, CC1, Chaos Theory, Engineering, Mouse-ear Cresses, sample population, incised, dSET/TAF-Ibeta, 2610030F17Rik, D Glutamine, Ion, Populus nigras, Populus trichocarpa Torr. & A.Gray, SSKS, c-Kit ligand, alpha-amino-gamma-methylmercaptobutyric acid, Peptid, assay, SCF, variable, AA407739projections, True Poplar, LFIS, Activity, single-organism developmental process, determination, Black, Cobalt, Pflanze, Mn-Superoxide Dismutase, Mn-Superoxide, protein, Cu-Superoxide Dismutase, GRO:0005338, 12Z)-9, viridiplantae, dominant Larsen syndrome, protein polypeptide chains, cis-Delta(9, Populus fremontii, halite, exact), ionic compounds, Msal-1, Fe-Zn Superoxide, Pro Oxidant, LARS1, Cobalt Superoxide, protein aggregate, Cu-Superoxide, multicellular organismal biosynthetic process, portion of tissue, increased, single-organism biosynthetic process, Oxidoreductase, anabolism, Tissue, Salt, Fe-Zn Superoxide Dismutase, Msal, plants, proteins, Populus balsamiferas, Salz, sel, Cottonwoods, c-ros-1, salts, Black Poplar, cloruro sodico, Oxygen, salt, radix (exact), Populus nigra, Fe Superoxide Dismutase, climbing root (narrow), papilla, signaling process, TG, Cu Superoxide Dismutase, Larsen syndrome, 12Z)-octadecadienoate, single organism signaling, Iron Superoxide, auxins, rock salt, caliptra de la raíz (Spanish, anatomical protrusion, Dismutase, lateral, plural), Oxygen Radical, land plants, lamina, aerial root (narrow), flanges, Populus fremontius, Manganese Superoxide Dismutase, Populus, cis-9, results, shortened, Copyrights., common salt, Black Poplars, down regulation of NAD(P)H oxidase activity, ARABIDOPSIS LIPOXYGENASE 1, shelf, tissue portion, Linoleate Oxygen Oxidoreductase, simple tissue, 根 (Japanese, Ag Zn Superoxide Dismutase, Oxygen Radicals, 12)-octadecadienoate, Linoleate:oxygen 13-oxidoreductase, poplar, absence, 2-methyl-1, poplars, Spalt, Iron, poplar trees, ionic compound, cis, Radical, shelves, Plant, Z)-9, Fe-Superoxide Dismutase, pooled, Mn-SOD, root, natrii chloridum, balsamiferas, projection, cis-linoleate, ridge, downregulation of NAD(P)H oxidase activity, higher plants, HSPC192, MCF3, PIG44, spine, Superoxide, Manganese Superoxide, fremontius, Linoleate-Oxygen, Cottonwood, short, Balsam, Proteomes, accessory, Manganese, 3-butadiene, biological signaling, localised, Cobalt Superoxide Dismutase, Mn SOD, Fe-Zn, lamellae, Gene, Reactive Oxygen Intermediates, 12-octadecadienoate, biosynthesis, lipoxygenase 1, Poplar, protein-containing complex, supernumerary, process of organ, Balsam Poplar, protrusion, lamella, True Poplars, ILFS1, polypeptide chain, Gene Products, Fe Zn Superoxide Dismutase, LIPOXYGENASE, (Z, nigras, Reactive, Lipoxygenase 1, Fe-Superoxide, Ag-Zn Superoxide, Lipoxygenase 2, plantae, 根冠(=kalyptra) (Japanese, formation, absent from organism, stubby, chlorure de sodium, Hemocuprein, ridges, Oxygen Species, synthesis, rootcap (exact), linoleic acid, (9Z, Active Oxygen Species, sels, autosomal dominant Larsen syndrome, Kochsalz, LEURS, Pro-Oxidant, Active Oxygen, laminae, Superoxide Dismutase, Erythrocuprein, radices (exact, Pro Oxidants, Lipoxygenase-2, True, Lipoxygenase-1, raíz (Spanish, LRS, protein complex, Balsam Poplars, sales, anatomical process, Proteins, total expressed protein, ion(1-), inhibition of NAD(P)H oxidase activity, function, Poplars, all, Iron Superoxide Dismutase, development, down-regulation of NAD(P)H oxidase activity, Temperatures, native protein, Natriumchlorid, natural protein, Linoleate-Oxygen Oxidoreductase, Ag-Zn Superoxide Dismutase, chemical analysis, Protein, Lipoxidase, 12-octadecadienoic acid, Active, table salt, focal, ROS, RNTLS, NaCl, flange, organ process, hr025Cl, Populus balsamifera, Sal, Ovoperoxidase, increased number, Populus L., B130022O04Rik, Salze, signalling, sal, Protein Gene Products, ATLOX1, present in greater numbers in organism, processes, process, Gene Proteins, signalling process, Superoxide:superoxide oxidoreductase, Populus nigras, Dietary Sodium, Pro-Oxidants, processus, LEUS, assay, 12Z)-octadeca-9, Ag-Zn, 12-dienoate, General activity, Mn Superoxide Dismutase, sodium chlorideprojections, True Poplar, LFIS, Activity, single-organism developmental process, determination, Black, FON1, Cobalt, Pflanze, Mn-Superoxide Dismutase, Mn-Superoxide, protein, Cu-Superoxide Dismutase, FLORAL ORGAN NUMBER 1, GRO:0005338, 12Z)-9, viridiplantae, ter, SUB, dominant Larsen syndrome, protein polypeptide chains, cis-Delta(9, Populus fremontii, halite, exact), DmelCG12298, ionic compounds, SUP, Msal-1, SCRAMBLED, Fe-Zn Superoxide, Pro Oxidant, LARS1, Cobalt Superoxide, protein aggregate, KIF20A, Cu-Superoxide, multicellular organismal biosynthetic process, portion of tissue, increased, single-organism biosynthetic process, Oxidoreductase, simple tissue., anabolism, Tissue, Salt, Bdr, Fe-Zn Superoxide Dismutase, Msal, plants, proteins, Super protein, Populus balsamiferas, Salz, sel, Cottonwoods, c-ros-1, sp, SNAP-25, salts, Black Poplar, cloruro sodico, Oxygen, salt, radix (exact), Populus nigra, Fe Superoxide Dismutase, Bsg75C, climbing root (narrow), papilla, signaling process, TG, Cu Superoxide Dismutase, Larsen syndrome, 12Z)-octadecadienoate, single organism signaling, Iron Superoxide, auxins, rock salt, caliptra de la raíz (Spanish, anatomical protrusion, Dismutase, lateral, plural), Oxygen Radical, land plants, STRUBBELIG, lamina, aerial root (narrow), flanges, Populus fremontius, Manganese Superoxide Dismutase, Populus, cis-9, results, shortened, common salt, Black Poplars, down regulation of NAD(P)H oxidase activity, ARABIDOPSIS LIPOXYGENASE 1, Term, shelf, tissue portion, Linoleate Oxygen Oxidoreductase, GENA70, simple tissue, 根 (Japanese, Ag Zn Superoxide Dismutase, Oxygen Radicals, 12)-octadecadienoate, Linoleate:oxygen 13-oxidoreductase, poplar, absence, 2-methyl-1, poplars, Spalt, Iron, poplar trees, ionic compound, cis, Radical, shelves, Plant, Z)-9, Fe-Superoxide Dismutase, pooled, Mn-SOD, root, natrii chloridum, balsamiferas, projection, cis-linoleate, ridge, downregulation of NAD(P)H oxidase activity, higher plants, HSPC192, MCF3, PIG44, spine, Superoxide, Manganese Superoxide, fremontius, Linoleate-Oxygen, Cottonwood, short, Balsam, Proteomes, accessory, FLORAL DEFECTIVE 10, Manganese, AT1G11140, 3-butadiene, biological signaling, localised, Cobalt Superoxide Dismutase, Mn SOD, Fe-Zn, lamellae, Gene, Reactive Oxygen Intermediates, 12-octadecadienoate, SUPERMAN, biosynthesis, lipoxygenase 1, Poplar, protein-containing complex, supernumerary, process of organ, CG12298, Balsam Poplar, protrusion, lamella, True Poplars, ILFS1, polypeptide chain, INSDC_qualifier:promoter, Gene Products, Fe Zn Superoxide Dismutase, LIPOXYGENASE, (Z, nigras, Reactive, Lipoxygenase 1, Fe-Superoxide, Ag-Zn Superoxide, Lipoxygenase 2, plantae, 根冠(=kalyptra) (Japanese, formation, mei-1794, absent from organism, stubby, chlorure de sodium, Hemocuprein, ridges, Oxygen Species, synthesis, Mif1, DmelCG4216, rootcap (exact), linoleic acid, (9Z, Active Oxygen Species, sels, autosomal dominant Larsen syndrome, Kochsalz, LEURS, SRF9, Pro-Oxidant, Active Oxygen, laminae, promoter sequence, Superoxide Dismutase, Erythrocuprein, radices (exact, Pro Oxidants, Lipoxygenase-2, True, Lipoxygenase-1, raíz (Spanish, LRS, protein complex, Balsam Poplars, sales, anatomical process, Proteins, total expressed protein, ion(1-), inhibition of NAD(P)H oxidase activity, Synaptosomal-associated 25 kDa protein, function, Poplars, all, STRUBBELIG-RECEPTOR FAMILY 9, Iron Superoxide Dismutase, development, down-regulation of NAD(P)H oxidase activity, Temperatures, native protein, Natriumchlorid, natural protein, Linoleate-Oxygen Oxidoreductase, Ag-Zn Superoxide Dismutase, chemical analysis, Protein, Lipoxidase, 12-octadecadienoic acid, Active, table salt, focal, ROS, RNTLS, NaCl, INSDC_feature:regulatory, flange, organ process, hr025Cl, Populus balsamifera, Sal, Ovoperoxidase, increased number, Dub, Populus L., CG4216, B130022O04Rik, Salze, FLO10, HERP, signalling, sal, Protein Gene Products, ATLOX1, present in greater numbers in organism, processes, process, Gene Proteins, signalling process, Superoxide:superoxide oxidoreductase, Populus nigras, Dietary Sodium, Pro-Oxidants, processus, LEUS, assay, 12Z)-octadeca-9, Ag-Zn, 12-dienoate, T19D16.8, General activity, SCM, Mn Superoxide Dismutase, sodium chlorideNip40-1, 3-butadiene, VAN, Van, HB-6, radices (exact, DCT1, ABIN-1, DMT1, AU018810, BELLRINGER, raíz (Spanish, plural), T7H20.80, aerial root (narrow), LARSON., biosynthesis, ABIN, GRO:0005338, PNY, mk, all, van, l(3)04276, BLH9, LSN, exact), hVAN, BEL1-LIKE HOMEODOMAIN 9, 根 (Japanese, DmelCG32315, CG1977, Naf1, NAF1, CG32315, Virion-associated nuclear shuttling protein, multicellular organismal biosynthetic process, A20-binding inhibitor of NF-kappa-B activation 1, single-organism biosynthetic process, mVAN, 2-methyl-1, VAAMANA, BLR, PENNYWISE, formation, anabolism, Dlt, Nef-associated factor 1, dre1, root, ABIN1, Nramp2, synthesis, radix (exact), nip40-1, climbing root (narrow), Nef, l(3)62Ba, T7H20_80, l(3)dre1, REPLUMLESS, HIV-1 Nef-interacting proteinfalseRoot isoprene formation alters lateral root development Maja Miloradovic van DoornIsoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analyzing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non-emitting poplar roots revealed an accumulation of O2 - in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signaling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S-lipoxygenase, in isoprene non-emitting poplar roots. Together our results indicate for isoprene a ROS-related function, eventually co-regulating the plant-internal signaling network and development processes in root tissue. This article is protected by copyright. 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