Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-13.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-7.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-9.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-8.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-18.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-18.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-12.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-16.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-16.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-7.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-20_180825101019.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-20.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-17.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-17.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-12.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-9.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-11.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-15.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-15.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-14.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-8.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-14.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-10.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-19.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_1-3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023048/180490-01_2-19.rawprimaryOK200longhongyu@csu.edu.cnHongyu LongMass SpectrometryShotgun proteomicsNot availableDentate gyrusQuantitative proteomicsHippocampal sclerosisTemporal lobe epilepsyItraqhttp://www.ebi.ac.uk/pride/archive/projects/PXD023048BrainThe study was carried out on 7 TLE patients subjected to standard hippocampectomy at the Department of Neurosurgery of Xiangya Hospital. And all patients went through comprehensive medical history, physical examination, brain magnetic resonance imaging (MRI) and electroencephalograph(EEG). Preoperative MRI and routine postoperative histopathologic examination of resected tissue confirmed HS. Written informed consent was obtained from all enrolled participants. The study was conducted following the guideline for the research involving humans, and approved by the Ethics Committee of Central South University, Xiangya School of Medicine and the affiliated Xiangya Hospital.Hippocampal samples of control group were obtained from autopsied patients of Xiangya School of Medicine, and exclusion criteria were patients with neuropsychiatric disorders and abnormities in the central nervous system(CNS) based on clinical history or histological examination. The resected brain tissues were snap-frozen in liquid nitrogen and then stored at -80˚C. Immunohistochemical staining against NeuN (Merck-Millipore, MAB377, 1 : 1000) was performed in brain slices, and for Nissl staining, brain slices were placed under agitation in cresyl violet staining solution. Staining results as localization reference, experienced pathologist therefore separated the DG from the rest of the hippocampus by microdissection . Quantitative 8-plex iTRAQ proteomic analysis was carried out on eight independent protein samples. Total proteins were extracted with iTRAQ lysis buffer, processed of disulfide bond cleavage and reductive alkylation of proteins with DTT and iodoacetamide, and then the proteins were hydrolyzed by trypsin. The pooled samples were labeled using the iTRAQ kit according to the manufacturer’s protocol (Applied Biosystems Sciex). The iTRAQ-labeled samples were mixed and resuspended in buffer A(2% acetonitrile, pH 10) and eluted with a 2% to 95% linear gradient of buffer B (90% acetonitrile, pH 10) over 90 min with a flow rate of 1 mL/min. The samples were separated into 20 fractions on a C18 column with HPLC system (Thermo Fisher Scientific) and analyzed using a Q Exactive Plus hybrid quadrupole-orbitrap Mass Spectrometer (Thermo Fisher Scientific).Tandem mass spectra were searched for computational analysis in Homo sapiens protein database using the Proteome Discoverer software. The Sequest algorithm was used to search with a fragment ion mass tolerance of 0.02 Da and a parent ion tolerance of 15 PPM. An FDR of less than 1% and with at least 2 unique peptides were used in identifying and quantifying proteins. The analysis of covariance (ANCOVA) model was hired for further adjusting for age. The protein threshold was set to achieve 95% confidence, P value and fold change cut-offs were used to classify as up-regulated protein (p<0.01 and fold-change>2) or down-regulated protein (p<0.01 and fold-change<0.5). Volcano plot and hierarchical cluster analysis were performed with the software R (Version: 3.5.1).PrideiTRAQiTRAQ8plex-116 reporter+balance reagent acylated residueDAVID Bioinformatics Resources 6.8(https://david.ncifcrf.gov/) was used to conduct enrichment analysis of DEPs and module proteins, and the underlying mechanism was identified in the context of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and gene ontology (GO) terms, which consists of cellular components(CC), molecular functions(MF) and biological processes(BP). p value <0.05 and gene count ≥2.0 were set as the threshold of significance. Protein-protein interaction(PPI) network was constructed using STRING(https://string-db.org/). Based on the STRING database, the interaction network was then visualized by Cytoscape software 3.0. Moreover, Molecular Complex Detection (MCODE) app in Cytoscape was utilized to screen the entire PPI network for hub genes modules, with degree cutoff ≥2, node score cut-off ≥0.2, K-Core ≥2 and Max Depth = 100.ProteomicsHongyu LongQ ExactiveDepartment of Neurology, Xiangya Hospital, Central South UniversityPARTIALHomo Sapiens (human)Not availablelonghongyu@csu.edu.cnDepartment of Neurology, Xiangya Hospital, Central South UniversityChinadalpha-SNAP, MeCN, Laboratory, Ass-1, Exams, Cerebrospinal Axis, Physical, Consent, Fox-1 homolog C, Exam, Medical Specialties, 5730420M11Rik, Chemical Shift Imagings, Pathologist, Polypeptides, protein polypeptide chains, KL receptor activity, Formations, CH3-C#N, Microdissections, B1, SCO5, Magnetic Resonance Imaging, fold, Chemical, SCO1, establishment and maintenance of substrate location, Software Engineering, Analysis, Research Activity, Gsfsow3, Laboratory Research, dJ1068F16.2, WMS, CG1216, Priorities, SET, Spin, Divorced, Analyses, ClinicalHistory, N, phosphatase 2A inhibitor I2PP2A, Tissue, Divorces, proteins, W, suprasegmental levels of nervous system, SNAP-25, HPLC, AI047805, DmelCG4299, set, column, medicine, D1, Medicine, Bs, CT, Homo sapiens disease, Functional MRIs, Echo Imaging, autolysin activity, Histological Labeling, SGS, Issue, suprasegmental structures, Ammon, Ct, Research Priorities, Natural Law, historical aspects, ethical aspects, seahorse, Software Tools, ACMICD, Medical Speciality, PBT, Computer Applications, Imagings, Stickstoff, Hippocampal, Dm1, Algorithm, Computer Applications Software, Zeugmatography, simple tissue, University, Subiculum, Research and Development, pbt, Software Applications, HLA-DR-associated protein II, DI-2, Source Software, I-2Dm, pooled, Chemical Shift, inhibitor of granzyme A-activated DNase, study protocol, beta Trypsin, Activities, I-2PP1, Spin Echo Imaging, Applications, nitrogen, TAF-IBETA, historical notes, RIC4, bacteriolytic toxin activity, Hexaribonucleotide-binding protein 3, TAF-Ibeta, methyl cyanide, humans, establishment and maintenance of cellular component location, krk1, Computer Software Applications, AA407358, Policies, l(1)VE614, Aspect, Image, number, precursor, cresyl violet, protein-containing complex, body system, CG7826, Human, Moral Policy, HRNBP3, Medical Specialities, Functional, Gene Products, CG7835, fMRI, disease or disorder, CG6625, CG42273, system, Steady State Free Precession MRI, Medical, Cornu Ammonis, Man, Application, RGD1560070, Hippocampus, Open Source Softwares, FOX3, monoperchlorate, anatomical systems, CNS, Software Application, beta-Trypsin, Open Source Software, Magnetic Resonance, 2pp2a, hippocampus major, kf, CG10574, Solution, NMR Tomography, Computer Software Application, drugs, 2PP2A, Tools, FOX-3, dSET, dSet, peptidos, Development and Research, Insurance, Shift Imaging, PTHB1, Horn, Law, proto-oncogene c-Kit, DmelCG6625, grupo, 1-(14)C-labeled, MRIs, Metaethics, NMR Imaging, Proper, Proteins, disorders, Hippocampus Propers, MR Tomography, Hippocampal Formation, Stainings, acetonitrile, buffer, encephalon, Magnetic Resonance Images, Tool, polypeptide, MR, archipallium, native protein, I-2PP2A, KIT ligand receptor activity, C18, cerebrospinal axis, chemical analysis, Dm I-2, proteomic analysis, condition, MFS1, Scans, Cluster Analyses, Hippocampus Proper, XKrk1, AU020952, Issues, holin, Proton Spin, Separations, Magnetic, CNS - Brain - Hippocampus (MMHCC), azote, ethanenitrile, CD117, ME-IV, plan specification, Computer Programs, Gene Proteins, hippocampus, localisation, Applications Softwares, C-Kit, Cerebrospinal Axi, Ssm, xkl-1, quantitative, IPP2A2, Activity, NCMe, determination, Ammon horn fields, Physical Exam, SEC9, cleavage, Imaging, protein, Xkl-1, Tomography, bA416N4.2, lysis, Resonance Image, Central Nervous, Alkylations, peptide, Speciality, Cut, Gsfsco1, Nervous Systems, peptido, diseases, Ethical Issues, l(1)7Ba, Specialities, l(1)7Bb, MRI Scans, AA408052, diseases and disorders, Min, synganglion, cresyl violet acetate, protein aggregate, Gsfsco5, alpha-SNAP, Computer Program, DmelCG42273, SOW3, Ethical Issue, portion of tissue, human disease, Insurance Medicine, Man (Taxonomy), reference sample, peptides, TAF-I, Natural, Rests, Open, human protein, min, Computer Programs and Programming, Ethical, mAPC, Histories, Ammons Horn, CG11387, ASS, IGAAD, DmelCG10574, Ammons horn, ppm, NMR, Medicines, necrosis, Research Priority, Sl, GPHYSD2, Functional MRI, Physical Examinations, ammon gyrus, phapii, establishment and maintenance of substance location, Specialties, DmelCG1216, formatio hippocampi, Modern, Moral Policies, Dmel_CG7826, StF-IT-1, Neun, Ammon Horn, Tr-kit, Labeling and Staining, hippocampus proper, Fox-3, Central Nervous Systems, results, Source Softwares, Programs, Subiculums, Neurosurgeries, Program, single organism localization, XBR, Situational Ethics, Specialty, Computer Applications Softwares, tissue portion, kl1-A, major hippocampus, bacteriocin activity, NeuN, NEUN, Ammon's Horn, Softwares, KIT, BcDNA:GH10590, Scan, Dmel_CG7835, Mnb, MNB, parent ion, tyrosine-protein kinase Kit, Clusterings, Steady-State Free Precession MRI, Ammon's, Ammon's horn, systema nervosum centrale, Encephalon, Ethics, Situational, Physical Examinations and Diagnoses, alphaSnap, kit, MASS, CG4299, AW124434, organ system, Insurance Medicines, disease, primal cortex, lysin activity, Labelings, precursor ion, Patient, Cluster, Egoism, Acetamide, Computer Software Applications., Clustering, Axi, Magnetic resonance imaging, Polypeptide, 7N, i2pp2a, Gruppe, the brain, other disease, dalphaSNAP, Neuna60, MRI Scan, Psychomotor agitation, Histological Labelings, ammon horn, SCF receptor activity, FBN, Gene, Laws, Computer, Spin Echo Imagings, presence, PHAPII, Cerebrospinal, Buffer, method, Homo sapiens, polypeptide chain, scfr, template-activating factor I, ECTOL1, l(3)77ABa, method used in an experiment, establishment and maintenance of position, Histological, l(3)77ABc, NRSF, RIC-4, Separated, SCFR, cresyl violet nitrate, study, Echo Imagings, Nervous System, Fdc, MRI, Mri, Staining, Research, clinical information, hippocampus (Crosby), Physical Exams, Magnetic Resonance Image, ipp2a2, Magnetization Transfer Contrast Imaging, Examination, non-neoplastic, OCTD, 10^[-6], DmelCG11387, Moral, grupos, taf-ibeta, Informed, Clients, Neuronal nuclei, disorder, liquid, 2-iodo-, Controlled, Policy, Applications Software, Cornu ammonis, Open Source, Controlling, DYRK1, dSNAP, neuraxis, Computer Software, Central, 3H-labeled, Medical Specialty, protein complex, igaad, 2610008J04Rik, TelN, Functional Magnetic Resonance Imaging, medical condition, Historical Aspects, dsnap, Hospital, Client, Historical Aspect, Labeling, Peptide, group, count in organism, Software Tool, EEG, Priority, Chemical Shift Imaging, natural protein, Examinations, Shift Imagings, Systems, Protein, Research Activities, I2PP2A, establishment and maintenance of localization, Dyrk1, connected anatomical system, Software, ACETONITRILE, cyanomethane, WMS2, Data Base, mononitrate, cornu ammonis, Separation, hippocampus proprius, c-KIT, AL023049, Propers, CC1, Engineering, Rest, Tripcellim, single-organism localization, Axis, nitrogeno, Protein Gene Products, incised, Natural Laws, Trypure, dSET/TAF-Ibeta, c-kit, 2610030F17Rik, Proton Spin Tomography, SNAP, Modern Man, SSKS, ResT, Peptid, assay, Aspects, Hrnbp3, Spin Echo, AA407739, groupe, Hippocampal Formations, Formation, Historical, snap, presence or absence in organismprojections, Amyloid intracellular domain 57, APP, Amyloid intracellular domain 59, ABETA, IPP2A2, Materials, Gene Ontology Projects, degree (angle), dmMOF, determination, primitive node, A4, CTFgamma, protein, Amyloid intracellular domain 50, Abpp, 5730420M11Rik, AAA, DmelCG9648, Cvap, Cut, protein polypeptide chains, CG42318, AG, Beta-amyloid protein 42, cerebral vascular amyloid peptide, dorsal marginal zone, l(1)7Ba, l(1)7Bb, dMOF, Beta-amyloid protein 40, Project, node, Software Engineering, protein aggregate, Gpi-1, amyloidogenic glycoprotein, hel-n1, AID(50), Computer Program, SET, Ag, PPI, Genomes, TAF-I, Nlk, dMax, phosphatase 2A inhibitor I2PP2A, Open, Computer Programs and Programming, proteins, CG11387, DmelCG4299, Alzheimer disease amyloid A4 protein homolog, N-APP, set, IGAAD, Gamma-secretase C-terminal fragment 50, DmelCG10574, Gamma-secretase C-terminal fragment 57, papilla, Gamma-secretase C-terminal fragment 59, Hensen node, AD1, Hensen's node, CT, Abeta, APPI, C80, embryo organizer, NK/GPI, C83, phapii, Gpi, Nervous system-specific RNA-binding protein Hel-N1, anatomical protrusion, AICD-50, S-APP-alpha, lamina, Ct, CG17144, flanges, StF-IT-1, CR32097, Gamma-CTF(57), Source Softwares, Software Tools, Programs, bHLHd7, bHLHd6, C99, bHLHd9, Program, Computer Applications, bHLHd8, el-1, bHLHd5, bHLHd4, count, mOC-X, Computer Applications Software, shelf, dmax, CG9648., Computer Applications Softwares, AICD-57, Genetic Materials, Henson's node, Ontology Projects, HuB, NK|GPI, Softwares, BcDNA:GH10590, ELAV-like neuronal protein 1, Genetic Material, AICD-59, Cerebral vascular amyloid peptide, Gene Ontologies, Ontology, ORG, Org, HELN1, Software Applications, Ontologies, HLA-DR-associated protein II, Projects, DI-2, Source Software, shelves, I-2Dm, Gpi1-r, Hub, HUB, Gpi1-s, amyloid precursor protein, INSDC_feature:gene, CG4299, alzheimer disease amyloid A4 protein homolog, inhibitor of granzyme A-activated DNase, Alzheimer disease amyloid protein, elrB, Gpi1-t, Protease nexin-II, projection, Dmel_CG5620, ridge, I-2PP1, pre-pulse inhibition, Applications, MOF, Mof, TAF-IBETA, Material, spine, p45, DMZ, Cistron, TAF-Ibeta, hub, i2pp2a, Bglap-rs1, amyloid-beta (A4) precursor protein, Gpi1s, Computer Software Applications, l(1)VE614, amyloid-beta A4 protein, DmelCG42318, AI461847, lamellae, P3(40), nucleocapsid, Gene, amyloid beta A4 protein, Computer, protein-containing complex, betaApp, elavl2, process of organ, Gene Ontology Project, Gene Ontology, PHAPII, Nervous system-specific RNA-binding protein Mel-N1, protrusion, AID(59), lamella, DmelCG3025, polypeptide chain, template-activating factor I, APP-C57, APP-C59, Gene Products, alzheimer disease amyloid protein, protease nexin-II, Pgi, heln1, Application, shield, Open Source Softwares, MAX, Genetic, PreA4, Gpi-1r, nodus primitivus, Gpi-1s, Software Application, ipp2a2, Phi, Gpi-1t, Open Source Software, 2pp2a, S-APP-beta, Dmel_CG17144, CG5620, Spemann's organizer, ridges, Gamma-CTF(59), kf, CG10574, Computer Software Application, AID(57), max, 2PP2A, Tools, DmelCG11387, taf-ibeta, stem node, dSET, dSet, organizer, laminae, Applications Software, Open Source, Encyclopedias, Amyloidogenic glycoprotein, Computer Software, protein complex, E030013M08Rik, HEL-N1, anatomical process, Proteins, igaad, CG3025, MF, embryonic organizer, Cistrons, Amf, Tool, AI875693, Gamma-CTF(50), APP-C99, Software Tool, native protein, natural protein, I-2PP2A, Adap, chemical analysis, Protein, Dm I-2, I2PP2A, Spemann Mangold organizer, core, Software, flange, organ process, NK, Data Base, Ontology Project, P3(42), PN-II, xel-1, nodal stem, Engineering, Soluble APP-beta, arc degree, Hu-antigen B, Protein Gene Products, C31, incised, Computer Programs, Gene Proteins, processes, process, dSET/TAF-Ibeta, Xel-1, 2610030F17Rik, Applications Softwares, AA960152, Soluble APP-alpha, processus, embryonic shield, assay, Beta-APP42, PN2, Beta-APP40, AA407739, CVAP, mel-N1, ABPPTLE - Temporal lobe epilepsy, d230, single-organism developmental process, Sector CA4, determination, region or site annotation, localisation-related epilepsy, number, A4, CA4 Region, Gene, dTAFII250, protein, partial epilepsy, protein-containing complex, EfW1, presence, TYPE, epilepsies, Lateral Temporal Epilepsy, Gyrus, Intervention or Procedure, DAGA4, temporal lobe, dmTAF[[II]]230, protein polypeptide chains, Readability, polypeptide chain, dmTAF1, Fascia Dentata, Taf230, Gene Products, dentate gyrus, MAM, protein aggregate, Cornu Ammonis 4 Area, SCG3, adult, Fascia, Hilus of the Fascia Dentata, partial, TAF250, Intellectual impairment, study, Taf200, network topology analysis, positional, Postmortem Examination, dTAF[[II]]250, Hippocampal CA4, reference sample, Post-Mortem Examinations, TFIID TAF250, focal epilepsy, hippocampal dentate gyrus, cel, interventionDescription, cell, Cognitive deficits, Psychomotor seizure, dentate area (dentate gyrus), CA4 Field, Area Dentata, Interventional, Temporal lobe epilepsy (disorder), area dentata, Taf1p, present in organism, proteins, familial temporal lobe, Uncinate Epilepsy, Cognitive defects, Examination, Uncinate, dTAF250, CA4 of Lorente de No, high frequency, Temporal lobe epilepsy (disorder) [Ambiguous], SURGICAL AND MEDICAL PROCEDURES., Clients, Hippocampal CA4 Region, site, epilepsy of temporal lobe, Postmortem Examinations, Post-Mortem Examination, TAF, gyrus dentatus, dentate area, Dentate Fascia, Controlled, epilepsy, Scleroses, Intervention Strategies, Controlling, data, Temporal Lobe Epilepsies, CA4 Field of Hippocampal Formation, dTAF[[II]]230, TAF[[II]]250, protein complex, Proteins, TAF200, l(3)84Ab, CA4, BG:DS00004.13, TAFII-250, Procedure, TAF250/230, Client, Post-Mortem, Cell, LGMD2C, dTAF230, temporal lobe epilepsy, development, count in organism, TAFII250, Dentatas, Postmortem, fascia dentata, native protein, Hippocampal, natural protein, p230, Examinations, Lateral Temporal, chemical analysis, Protein, Epilepsies, TAF[[II]]250/230, sequence, Area, TFIID, Temporal Lobe, Cognitive abnormality, Area Dentatas, Region, Adults, Hilus Gyri Dentati, region, Intervention, Taf[[II]]250, Dentata, Hippocampal Sector CA4, Epilepsy, adults, TAF[[II]]230, frequent, Hilus of Dentate Gyrus, positional polypeptide feature, DMDA1, resistant, TLE, Dentate, Uncinate Epilepsies, Field, Gyrus Dentatus, common, TAF[II]250, Childhood, Understanding, CG17603, TAF[[II]], primary structure of sequence macromolecule, Cognitive impairment, Protein Gene Products, Benign Psychomotor Epilepsy, Gene Proteins, Autopsies, DMDA, DmelCG17603, Patient, Taf250, partial epilepsies, SR3-5, SCARMD2, Hippocampal Sector, Abnormality of cognition, Benign Psychomotor, assay, Childhood Benign Psychomotor Epilepsy, quantitative, Post Mortem Examination, Hippocampal CA4 Field, Lateral Temporal Epilepsies, TAF230, presence or absence in organism, TAF1epilepsy, TLE - Temporal lobe epilepsy, CA4 Field of Hippocampal Formation, Temporal Lobe Epilepsies, Sector CA4, CA4 Region, CA4, Gyrus, Lateral Temporal Epilepsy, temporal lobe epilepsy, temporal lobe, Dentatas, Scleroses., fascia dentata, Hippocampal, Fascia Dentata, Lateral Temporal, proteomic analysis, Epilepsies, Area, dentate gyrus, Temporal Lobe, Area Dentatas, Region, Cornu Ammonis 4 Area, Hilus Gyri Dentati, Fascia, Hilus of the Fascia Dentata, Dentata, Hippocampal Sector CA4, Epilepsy, Hilus of Dentate Gyrus, Hippocampal CA4, hippocampal dentate gyrus, TLE, Dentate, Uncinate Epilepsies, dentate area (dentate gyrus), CA4 Field, Psychomotor seizure, Field, Area Dentata, Gyrus Dentatus, area dentata, Temporal lobe epilepsy (disorder), Childhood, familial temporal lobe, Uncinate Epilepsy, Uncinate, Benign Psychomotor Epilepsy, CA4 of Lorente de No, Temporal lobe epilepsy (disorder) [Ambiguous], Hippocampal CA4 Region, Hippocampal Sector, Benign Psychomotor, Childhood Benign Psychomotor Epilepsy, epilepsy of temporal lobe, Hippocampal CA4 Field, gyrus dentatus, dentate area, Dentate Fascia, Lateral Temporal EpilepsiesfalseiTRAQ-based proteomic analysis of dentate gyrus in temporal lobe epilepsy with hippocampal sclerosisTemporal lobe epilepsy (TLE) is the most frequent type of focal epilepsy in adults, typically resistant to pharmacological treatment and mostly present with cognitive impairment and psychiatric comorbidities. The most common neuropathological hallmark in TLE patients is hippocampal sclerosis(HS). However, the underlying molecular mechanisms involved remain poorly characterized. Dentate gyrus(DG), one specific hippocampal subarea, structural and functional changes imply a key involvement of the DG in the development of TLE. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) -based quantitative proteomic technique was performed to analysis of hippocampal DG obtained from patients with TLE-HS compared to control samples obtained from the autopsy. Our proteomic data identified 5583 proteins, of which 82 proteins were up-regulated and 90 proteins were down-regulated. Bioinformatics analysis indicated that differential expressed proteins enriched in “synaptic vesicle”, “mitochondrion”, “cell-cell adhesion”, “regulation of synaptic plasticity”, “ATP binding” and “Oxidative phosphorylation”. Protein-protein interaction network analysis found a pivotal module of 10 proteins that relate to “Oxidative phosphorylation”. This study is the first to investigate proteomic alterations in DG region of TLE-HS patients, and pave the way to better understanding of epileptogenesis mechanisms and future therapeutic intervention.2021-01-182020-12-10PXD02304828065516460991804982953585111454224942024527872842188090368093270429330845811363336351524260119834300662758385NCBITaxon:128068888561896111430118726355557114526236493461364031952065126957366649274670738538836411354615821582310665688954762721123294422291180992323122586365458333460792519365914320597129386558195851149315242592637371250232NCBITaxon:1272111957748186614885843104300245135867221755437408169180454266834295319453058612668351498225058149636745855452811320814795641003923751463694204943320896420896344597413797912838440066793529367156759388036478434716052165994048321659721659545501315282693042165915627161696833218321729204277944762376454317203094980197525556978159509439843948840479NCBITaxon:2157525338551531051067439899037122857759515205968762262311210884555588845587077918178081736309431794571698936160488409859NCBITaxon:37162104782679401505907148364320421061320491381693630355543204663263331199456545351102541110321745921612928867641002642036945694101676341021076127735113165823925472793342814613584301417298174633457744491675476048932128735675684030135062191726518130194111929439367110411310239320255598632582788396591047593474951604901652998355256631758543980110243260866310245980875741755518320196447145268045796631367019083233115611415535510421932555217634981812359871069955529NCBITaxon:4751850298335476113615741465477180066556484337677299767982510216998238976484074137351155711089725482444474153NCBITaxon:5055789969844100036465349334147147493548019467512991143597412764731370863265478765698353221599838192011106775772710348198523487804794640986099586157187176685991915729513722117110129292107687834974198602028091488406334249234235152335985988655974649987039202111072943311711397215987419085037117082711708284182350241451139692931902197822474636046147123749402375939723287581722532566489050794526591972211915853101774208588596NCBITaxon:615750521028307989515586411051989972375059576131720002634196102641088878176179212190659407121906772275885815689964975151950582873757244NCBITaxon:22023717NCBITaxon:17036122019998570506174767927450627249159615333554184005246432136861505964878486662941786661347515363291018194301086731018510184NCBITaxon:1763573039635586331492862876705801321650852718481865368595105135553482260710185687294296110091416333NCBITaxon:619184023210007348824123363630710470058127187878645159812894555663577553470951451225786260704NCBITaxon:13356262607052607076584574054163677483167233150561007133705654195NCBITaxon:305550445791399878168119011928752829126806315327341478281055524NCBITaxon:9606135858469008111167815022830519302836728593008529005NCBITaxon:56938268830640179469915101184994352581515901448242838111394426041937047592723708604232816772850351581990119519532673863701370249240536088385816224881861568175626158355837153337605726711935019980861193502167879108579137113712724071906503714759172629815712562745757270903113335503728142433747924910078711082NCBITaxon:96157243359463318586260799141262200298492670NCBITaxon:126371928124921252830385683856959116926610065812375612750454266625515197881356638375064422336009412934979054632245352472140125751511649908515151438992374537474454464152432359376059202228870515805955365550881579327160293851578114978633892894535936479437NCBITaxon:6854412754225117159010901341673871589552101296610306129671585158451584937659393432715910022637673388990461423451287689591198332966467041464792936576164280341160221641331599280382803536909108917688149894621812141080348179392287889295027113133295282829445686174934681911265269972273218903821076012471904007271597369978281143114020641198108713554774365814245072295337969820423212422312916321803347087145372091645733215358157546992877702020603281143193382935508332912922114872526499972830357390871830872672710379085507176299707678926837041557372921405329412816078395639243142626548982273775962073220865958827355516936053307972551811354915974953502611595561432138406072587814144715094933491949634016143347280059360463164610466038151845474485003567097477771951240174841855601335755547920427460769946881037620148871005941289093164354282688500712303831389464940838283594144242322197857742171598921409212205853939621157781154756929793244704749906604162103591905158114452749907312987779913112273170187546991103589915NCBITaxon:3729610360983341871049113079813367953164072994671034015786582645303337330638301130641399253064236365059116965478854698112116203431710337286129940445862073401153571119529652611745531114716133865413131663430552132312017983602532373127612552329935129938505731631764261979401151345844577267872862321933415134618620862116079139222798351681196125522814953932232311676175731210807721348799994886185696281408434103351996321849620994887321392488209711533820961153392095330439375569927612686266447NCBITaxon:26970493398624369411031219588320509202906101306859997586444588286432499525297246426066741158343212717894278637894261477405256737863912334355141998349317998686554055343529148654514712454668892510429863571246791516413582576328840193733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