Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/proteinGroups.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/msms.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/mqpar.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_238_20210305_KS_OP259_lysate_24h_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_240_20210305_KS_OP261_lysate_24h_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_213_20210304_KS_OP247_nucl-Chr-Inf_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_214_20210304_KS_OP248_nucl-Chr-Inf_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_235_20210305_KS_OP258_lysate_ChrInf_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_228_20210305_KS_OP253_lysate_K_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_229_20210305_KS_OP254_lysate_K_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_239_20210305_KS_OP260_lysate_24h_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_208_20210304_KS_OP244_nucl-K_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_220_20210304_KS_OP252_nucl-24h_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_215_20210304_KS_OP249_nucl-Chr-Inf_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_230_20210305_KS_OP255_lysate_K_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_233_20210305_KS_OP256_lysate_ChrInf_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_234_20210305_KS_OP257_lysate_ChrInf_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_219_20210304_KS_OP251_nucl-24h_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_210_20210304_KS_OP246_nucl-K_3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_218_20210304_KS_OP250_nucl-24h_1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_209_20210304_KS_OP245_nucl-K_2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/uniprot_mg_s6_nc.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_230_20210305_KS_OP255_lysate_K_3.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_233_20210305_KS_OP256_lysate_ChrInf_1.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_234_20210305_KS_OP257_lysate_ChrInf_2.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_210_20210304_KS_OP246_nucl-K_3.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_220_20210304_KS_OP252_nucl-24h_3.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_209_20210304_KS_OP245_nucl-K_2.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_229_20210305_KS_OP254_lysate_K_2.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_239_20210305_KS_OP260_lysate_24h_2.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_214_20210304_KS_OP248_nucl-Chr-Inf_2.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_208_20210304_KS_OP244_nucl-K_1.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_215_20210304_KS_OP249_nucl-Chr-Inf_3.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_213_20210304_KS_OP247_nucl-Chr-Inf_1.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_228_20210305_KS_OP253_lysate_K_1.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_238_20210305_KS_OP259_lysate_24h_1.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_219_20210304_KS_OP251_nucl-24h_2.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_235_20210305_KS_OP258_lysate_ChrInf_3.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_218_20210304_KS_OP250_nucl-24h_1.indexftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD025107/Pobeguts_dif_240_20210305_KS_OP261_lysate_24h_3.indexprimaryOK200herr.romanoff@gmail.comAleksandr ZubovMass SpectrometryShotgun proteomicsMaxQuantIntracellular infectionLc-ms/msNucleoid-associated peoteinsShotgun proteomicsMycoplasma gallisepticumhttp://www.ebi.ac.uk/pride/archive/projects/PXD025107Mycoplasma gallisepticum S6 was cultivated in a medium containing tryptose 20 g/l, Tris 3 g/l, NaCl 5 g/l, KCl 5 g/l, yeast extract (10%, Helicon, Russia), horse serum (10%, Biolot, Russia), glucose 1% (Sigma) and penicillin (Sintez, Russia) with a final concentration 500 units/ml at pH 7.4 and 37 °C. Chicken erythroblast cell line HD3 (clone A6 of line LSCC51,52) was obtained from professor S. V. Razin (Institute of Gene Biology, Russian Academy of Sciences) and was cultivated as described previously [Iarovaia, O. V. et al. In embryonic chicken erythrocytes actively transcribed alpha globin genes are not associated with the nuclear matrix. J. Cell. Biochem. 106, 170–178 (2009)]. The gentamicin invasion assay was carried out as previously described [Winner, F., Rosengarten, R. & Citti, C. In vitro cell invasion of Mycoplasma gallisepticum. Infect. Immun. 68, 4238–4244 (2000)]. We used the concentration of gentamicin 600μg/ml. Cell lines were infected with the M. gallisepticum S6 in a ratio of 1:1000 and cultured for 24 hours (acute infection) or 4 weeks (chronic infection) in a CO2-incubator. After treatment with gentamicin, HD3 cells were removed from the plate, pelleted by centrifugation at 300g for 5 min, and diluted in 1 ml of mycoplasma medium. The resulting sample was serially diluted and plated on a semi-liquid medium. Colonies were cultivated to the late log phase and used to obtain a culture of M. gallisepticum to isolate the nucleoid fraction. We used a culture of M. gallisepticum with synchronized division. For this 1% of the post-infection cells was subjected to starvation for 9 h on a liquid medium containing tryptose 20 g/l, Tris 3 g/l, NaCl 5 g/l, KCl 5 g/l, and 500 units/ml penicillin (Sintez, Russia) in aerobic conditions at pH 7.4 and 37 °C. After that yeast extract (10%, Helicon, Russia), horse serum (20%, Biolot, Russia), glucose 1% (Sigma) were added. The culture was grown further at 37 °C to the logarithmic growth phase. M. gallisepticum nucleoid fractions were isolated using the method described by our group previously [Zubov, A. I. et al. Data on nucleoid-associated proteins isolated from Mycoplasma Gallisepticum in different growth phases. Data Br. 31, 105853 (2020)]. Sample preparation for proteomic analysis was performed as follows: 20 μl of 10% sodium and 2 μl of nuclease mix (GE HealthCare) were added to the nucleoid fraction and incubated for 1 hour at 4 ° C. After incubation, 80 μL of 100 mM Tris – HCl, pH 8.5 with protease inhibitor cocktail (GE HealthCare) was added to the sample. Protein concentration was estimated by BCA Assay (Sigma). Aliquots containing 300 μg of protein material were diluted to 1 μg / μL with 100 mM Tris-HCl, pH 8.5, and tris (2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma) and chloroacetamide (CAA, Sigma) were added to the final concentrations of 10 and 30 mM, respectively. Cys-reduction and alkylation were achieved by 10 min heating of the sample at 85 °C. Trypsin (Promega, USA) was added at a ratio of 1:100 w/w to protein amount and incubated at 37 °C overnight. Then the second trypsin portion 1:100 w/w was added, and the sample was incubated for 4 h at 37 °C. Proteolysis was stopped by adding trifluoroacetic acid to 1%. Precipitated CDNa was removed by ethyl acetate [Masuda, T., Tomita, M. & Ishihama, Y. Phase transfer surfactant-aided trypsin digestion for membrane proteome analysis. J. Proteome Res. 7, 731–740 (2008)]. Samples were subsequently purified on OASIS columns (Waters). LC-MS analysis was carried out on an Ultimate 3000 RSLC nano HPLC system connected to a QExactive Plus mass spectrometer (Thermo Fisher Scientific, USA). Samples were loaded to a home-made trap column 70 × 0.1 mm, packed with ProntoSil C18 120A sorbent (Altmann Analytik), in the loading buffer (2% ACN, 98% H2O, 0.1% TFA) at 10 μl/min flow and separated at RT in a home-packed fused-silica column 300 × 0.1 mm packed with Reprosil PUR C18AQ 1.9 um (Dr. Maisch, Germany) into the emitter prepared with P2000 Laser Puller (Sutter, USA) [Kovalchuk, S. I., Jensen, O. N. & Rogowska-Wrzesinska, A. FlashPack: Fast and simple preparation of ultrahigh-performance capillary columns for LC-MS. Mol. Cell. Proteomics 18, 383–390 (2019)]. Samples were eluted with a linear gradient of 80% ACN, 19.9% H2O, 0.1% FA (buffer B) in 99.9% H2O, 0.1% FA (solvent A) from 4 to 36% of solvent B in 1 h at 0.44 μl/min flow at RT. MS data were collected in DDA mode. MS1 parameters were as follows: 140 K resolution, 350–2000 scan range, max injection time 50 ms, AGC target 3 ×10^6. Ions were isolated with 1.4 m/z windows and 0.2 m/z offset targeting 10 highest intensity peaks of + 2 to + 6 charges, 8 ×10^3 minimum AGC, preferred peptide match, and isotope exclusion. Dynamic exclusion was set to 40 s. MS2 fragmentation was carried out in HCD mode at 17 K resolution with 27% NCE. Ions were accumulated for max 45 ms with target AGC 1 ×10^5.PrideLabel freeiodoacetamide derivatized residuemonohydroxylated residueacetylated residueIdentification and label-free quantification analysis were performed with MaxQuant 1.6.10.43 software with default settings. The data were searched against M. gallisepticum S6 NCBI database. Further calculations and visualizations were made in Python 3.7.10.ProteomicsGleb FisunovQ Exactive PlusPARTIALLab of Proteome Research, Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, RussiaMycoplasma Gallisepticum S6Not availablealexanderzubov@icloud.comLab of Proteome Research, Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, MoscowRussiaWater, Gentamicin, Materials, dmMOF, muriate of potash, ion, Glukose, alpha-Spe, BOUND WATER, growth and development, oxidane, alpha-Spc, CG31654, Solvent, Long Term, 4'-diaminoazobenzene-4-sulfonamide, WATER, HOH, Bca, DmelCG9648, 5730420M11Rik, dmTAF[[II]]230, Trifluoroacetate, Polypeptides, protein polypeptide chains, hydrogen chloride, cDNA library construction, Dextrose, Cultural, dMOF, B1, Line, l(2)SH1330, HCL-C, 3, AGM4, 4, 6, Sodium Ion, sylvite, CG18546, Pleuropneumonia Like Organisms, Russian Federation (Europe), peptidolysis, bca, SET, ethnicity, Divorced, C79325, TFIID TAF250, cel, weight-weight percentage, phosphatase 2A inhibitor I2PP2A, complementary DNA, Prontosil, Processed cyclic AMP-responsive element-binding protein 3-like protein 1, proteins, Divorces, T6G21.3, Fast, HPLC, AI047805, DmelCG4299, glucose, salt, set, Wasserstoffchlorid, h, column, scientific observation, ACN, sample, D1, Acn, n, Red, s, fused to, Infections and Infestations, Eperythrozoon, SIMPLE, FAST, accumulated, dTAF[[II]]230, Klor-con, Degradations, Stars, 7-pentadeoxy-6-(methylamino)heptopyranoside, Bovimyces, familial, TAF200, Longterm Effect, integral to membrane, Red Blood Cell, FASTK, Penicillin, ada, bHLHd7, bHLHd6, bHLHd9, bHLHd8, H2O, common salt, Cultural Background, D-Ada, bHLHd5, l(2)br3, bHLHd4, Dm1, Cultures, increased period, Streptocide (Evans), PIG7, CG9648., Chlorwasserstoff, Nuclear Matrices, sodium, Red Blood Corpuscle, dAP-2a, (alpha-D)-Isomer, leukemic reticuloendotheliosis, natrium, l(2)06694, D-Glucose, Acid, HLA-DR-associated protein II, DI-2, LY57, I-2Dm, Protein Digestions, FU, proportionality, gentamycins, rate, Infection and Infestation, INSDC_feature:gene, alpha, AP-2alpha, inhibitor of granzyme A-activated DNase, beta Trypsin, DmelCG4260, w/w, I-2PP1, TAF-IBETA, Taf250, Red Blood Cells, Material, Fu, TAF-Ibeta, Monopotassium chloride, hemocyanin, chlorane, fused, Glucose Monohydrate, STARS, TAF230, Old astrocyte specifically-induced substance, FUSED, knobbly, anon-EST:fe2E2, Glucose, Peptidomics, Effects, chlorure d'hydrogene, alpha-Spect, STK10, Fused, Nuclear Scaffold, protein-containing complex, thomson, body system, DmelCG4482, CG7826, agua, fs(3)neo61, mass-to-charge ratio, Ion Level, period, CG10422, DmelCG3025, Ly57, normoblast, Oasis, CG7835, Gene Products, CG42273, system, hemerythrin, Blood Corpuscle, PH3, Backgrounds, Cesium Trifluoroacetate, proportion, F15E12_6, MAX, anatomical systems, dTAF[[II]]250, Acinus, Hydrogen chloride, Longterm, cell, beta-Trypsin, dre3, 2pp2a, Sciences, Cultural Relativisms, Long-Term, Alpha-Adaptin, 6-diamino-3-[3-deoxy-4-C-methyl-3-(methylamino)pentopyranosyloxy]-2-hydroxycyclohexyl 2-amino-2, ions, study assay, Phasianus gallus, CG10574, CD156, acinusL, Injectable, dTAF250, max, hairy cell leukemia, 2PP2A, acinusS, Kochsalz, dSET, dSet, Customs, Chronic, peptidos, Antibiotics, globin, Kb, Anhydrous, Nuclear Scaffolds, PTHB1, l(3)dre3, Ki, measuring, mass percentage, alpha-Spectrin, grupo, CG4482, Proteins, CG3025, DmelCG31211, BG:DS00004.13, chloridohydrogen, Cultural Backgrounds, increased time, cDNA, buffer, Digestions, Cell, chronic, mKIAA0670, B930045J24, dTAF230, Kaliumchlorid, polypeptide, native protein, Natriumchlorid, Proteolyses, I-2PP2A, C18, chemical analysis, Long Term Effects, Dm I-2, proteomic analysis, high time, TAF[[II]]250/230, MENE(2L)-A, SMAP45, Sodium, MIXL, NaCl, alphaSp, ATCMPG1, MET, ATCMPG2, Sodium Ion Level, Embryonic, Taf[[II]]250, AU020952, chicken, sulfamidochrysoidin, mating_type_alpha, Separations, postnatal growth, Striated muscle activator of Rho-dependent signaling, Na, OASIS, Natrium, hydrogen phosphorus, CD156a, CG14732, Longterm Effects, Mycoplasma gallisepticum, ME-IV, plan specification, Gene Proteins, br3, AP2, RPD3-2, hydrogen phosphide, Bam-C, alpha mating type (yeast), quotient, Relativisms, bantam, NIP, Relativism, [OH2], Cultural Relativism, Sodium 23, hydrochloric acid, IPP2A2, BamF, AP-2, BamC, determination, sulfamidochrysoidine hydrochloride, postnatal development, classic hairy cell leukaemia, Infestations and Infections, Monohydrate, nip, KCl, Hydrogenchlorid, protein, (DL)-Isomer, extracted material, HCHWA, Alkylations, peptide, l(3)04276, Background, halite, Incubator, sodio, ClvPrd, peptido, BANF, Klotrix, kinky, alpha-adaptin, Min, 2', DmelCG10422, protein aggregate, Chicken, Injectables, Effect, DmelCG42273, MIX, alpha-spec, Penicillin Antibiotics, CG4260, peptides, ATP-dependent proteolysis, Scaffold, MUB3_18, TAF-I, dMax, 2610036I19Rik, membrane region, 2610510L13Rik, E430039A18Rik, min, mAPC, 35Bb, MUB3.18, Estimated, SCAN, Protein Degradation, DL-glucose, alpha-spectrin, genetic, cloruro sodico, IGAAD, fSAP152, Nucleoskeletons, DmelCG10574, trifluoro-, AI316800, dihydridooxygen, Lyw-57, blood capillary, associated, dihydrogen oxide, 3.5.1.98, Long-Term Effects, Scaffolds, ratio, Asteromyces <mycoplasmas>, [KCl], phapii, rock salt, PLATEST, PRO, Gallus gallus domesticus, aqua, Dmel_CG7826, CG9648, StF-IT-1, late, Th, ham, TAFII-250, classic hairy cell leukemia, membranous organ component, TAF250/230, anon-WO03070958.3, CYS, Gallus gallus, Acute onset, Nucleoskeleton, hereditary cerebral hemorrhage with amyloidosis - Dutch type, any method, SLP65, alphaSpec, Sodium-23, Ly-57, TAFII250, l35Bb, HCD, Ionen, Digestion, cg4260, dmax, HCL, Genetic Materials, Kaon-Cl 10, HD3, spectrin, and GLY protein 2, Genetic Material, Dmel_CG7835, Acetic acid, Lines, Mnb, MNB, MENE (2L)-A, MILD1, Yeast, Blood Cell, Haemobartonella, SLP-65, and GLY protein 1, membrane of organ, CHARGES, growth pattern, BASH, MS/MS, non-developmental growth, HCl, Trifluoroacetic, hydrogen hydroxide, CG4299, AW124434, natrii chloridum, CG17603, merged with, TAF[[II]], Pleuropneumonia, oxygen-carrying, surfactant, acqua, l(2)SH2 1330, chloracetamide, organ system, Red Blood Corpuscles, MOF, Mof, Gallus domesticus, Cesium, D Glucose, SR3-5, surface active agent, Erythrocyte, Mycoplasma putrefaciens, Cistron, Blood Corpuscles, Wasser, BG:DS01219.1, Polypeptide, i2pp2a, Proteomes, time, Platelets, Gruppe, gluco-hexose, alpha-Ada, d230, Beliefs, l(3)alpha-Spec, dutch hereditary cerebral amyloid angiopathy, Asterococcus <mycoplasmas>, RPD3, mol, Pleuropneumonia-Like Organisms, Degradation, surfactants, Matrix, Gene, dTAFII250, slow time, ACINUS, Matrices, EfW1, l(2)SH2 0460, PHAPII, Russian SFSR, Buffer, alpha-ada, method, polypeptide chain, template-activating factor I, dmTAF1, Ms1, Taf230, Injection, integral component of membrane, method used in an experiment, klo, chickens, F15E12.6, alpha-Sp, CG1977, Level, Separated, cloruro de hidrogeno, Pro-Mega, l(2)br23, TAF250, Taf200, Genetic, MS1, iones, MS2, ipp2a2, phosphane, chlorure de sodium, Borrelomyces, Taf1p, eau, Protein Degradations, Infestation and Infection, grupos, ATP:Fas-activated serine/threonine protein phosphotransferase activity, taf-ibeta, male sterility 1, region of membrane, liquid, Long-Term Effect, mKIAA1278, culture, TAF, TCEP, BLNK-S, TP53I7, membrane, DYRK1, data, TAF[[II]]250, potassium chloride, (beta-D)-Isomer, 3A9, protein complex, DmelCG1977, TSH1, l(2)35Bb, igaad, Cell Lines, total expressed protein, l(3)84Ab, NY-CO-33, Protein Digestion, Cistrons, Peptide, light amplification by the stimulated emission of radiation, group, Belief, AI875693, development, CAA, [HCl], Axin, Isotope, PPLO, capillary vessel, natural protein, p230, water, Protein, I2PP2A, Infection, Hydrochloride, whole membrane, TFIID, table salt, Nuclear, Dyrk1, prolonged period, connected anatomical system, nucleoskeleton, SDCCAG33, tandem MS, Blood Cells, Separation, transmembrane, 10^[-9], TAF[[II]]230, D-alphaAda, MALE STERILITY 1 PROTEIN, CC1, FCP-B, Rest, Tripcellim, cerebral amyloid angiopathy, TAF[II]250, CG15268, sample population, Anhydrous Dextrose, Protein Gene Products, cAMP-responsive element-binding protein 3-like protein 1, Trypure, dSET/TAF-Ibeta, 11Na, 2610030F17Rik, Russian S.F.S.R., DmelCG17603, Ion, concentration, l(3)62Bd, joined with, BAM, AA960152, Bam, l(2)SH0460, Globin, alfa-Spec, Peptid, assay, SCH, AA407739, growth, 1700112N14Rik, groupe, hereditary cerebral haemorrhage with amyloidosis - Dutch type, coalesced, Spec, sodium chloride, Glc, TAF1Applications Software, Dnmlp1, Open Source Softwares, Open Source, data, Software Applications, Computer Software, Dlp1, determination, Source Software, Software Application, Open, Drp1, Engineering, Open Source Software, Computer Programs and Programming, AI450666., Computer, 6330417M19Rik, free, Source Softwares, Software Tools, Computer Software Application, Tool, Programs, Computer Programs, python, Program, Computer Applications, Applications, Software Tool, Applications Softwares, Tools, chemical analysis, Computer Applications Software, Computer Applications Softwares, assay, Software Engineering, Softwares, Software, Computer Program, Application, Data Base, Computer Software ApplicationsButyl phthalate, host organism, Size, Enoyl-CoA hydratase 2, Respiratory conditions due to unspecified external agent, Single-Stranded DNA-Binding Protein, ALVEOL PNEUMONOPATHY NEC, Other diseases of respiratory system NOS, tef, disorder of respiratory system, Unspecified alveolar and parietoalveolar pneumonopathy, respiratory system disease or disorder, dmTAF[[II]]230, (3R)-hydroxyacyl-CoA dehydrogenase, RESP COND: EXT AGENT NEC, MFE-2, responsivity, 1, 4, Single-Stranded DNA Binding Proteins, [X]Other diseases of the respiratory system (disorder), present in fewer numbers in organism, RESP SYSTEM DISEASE NOS, [X]Chronic and other pulmonary manifestations due to radiation (disorder), respiratory system disorder, Cell Walls, Unspecified disease of respiratory system, Gene Expressions, TFIID TAF250, Genomes, disease or disorder of respiratory system, cel, Pneumoconiosis due to other inorganic dust, Single Stranded DNA Binding Proteins, Mollicutes, hypoplasia, Respiratory conditions due to other specified external agents, Respiratory System Disorder, DNA-Binding, Other respiratory system diseases (disorder), chromatid, DBP|GC, regulation of chromosome organization and biogenesis, DNA Binding Protein, Respiratory Disorder, DNA-Binding Protein, decreased, DNA Single-Stranded Binding Protein, Paramycetes, INORG DUST PNEUMOCON NEC, dTAF[[II]]230, Lung involvement in other diseases classified elsewhere, wide/broad, organisation, Regulation of Gene Expression, TAF200, Benzenedicarboxylic acid dibutyl ester, respiratory disease, Respiratory disorder, TAFII-250, TAF250/230, Respiratory system diseases NOS (disorder), Gene Action, Gene Expression, TAFII250, Expression Regulation, D-bifunctional protein, increased period, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, Sizes, NOS, Binding Protein, GRD3, SS DNA BP, Gc-globulin, Phthalic acid dibutyl ester, Factors, [X]Respiratory conditions due to unspecified external agent, dibutyl benzene-1, DNA Single Stranded Binding Protein, CG17603, Lung involvement in conditions classified elsewhere, ALVEOL PNEUMONOPATHY NOS, Other respiratory system diseases NOS, TAF[[II]], 4.2.1.107, Single Stranded DNA Binding Protein, Genome Sizes, wide, Taf250, Chromosome, SR3-5, Multifunctional protein 2, DNA, Regulation, 17-beta-hydroxysteroid dehydrogenase 4, TAF230, 4.2.1.119, 17-beta-HSD, [X]Chronic and other pulmonary manifestations due to radiation, d230, 2-benzenedicarboxylate, conformation, Gene, dTAFII250, slow time, broad, EfW1, Transcription Factor, Group-specific component, n-Butyl phthalate, MFP2, Other diseases of respiratory system, disease of respiratory system, reduced, dmTAF1, subnumerary, Taf230, Other specified alveolar and parietoalveolar pneumonopathies, Cell., tiny, Walls, respiratory system disease, TAF250, reactivity, study, SDR8C1, Taf200, Transcription, Other respiratory system diseases, dTAF[[II]]250, perMFE-2, birds, cell, Disease of respiratory system, Other respiratory system diseases NOS (disorder), VDBP, Taf1p, VDB, decreased number, VDBG, Expressions, [X]Respiratory conditions due to unspecified external agent (disorder), dTAF250, o-Benzenedicarboxylic acid dibutyl ester, LUNG INVOLV IN OTH DIS, regulation of chromosome organisation, Benzene-o-dicarboxylic acid di-n-butyl ester, Chronic, [X]Respiratory conditions due to other specified external agents, RESP SYSTEM DISEASE NEC, species, 3-alpha, Expression, Phthalic acid di-n-butyl ester, Di-n-butyl phthalate, TAF, relational structural quality, [X]Respiratory conditions due to other specified external agents (disorder), respiratory disorder, small, 7-alpha, 17[b]-HSD, TAF[[II]]250, Dibutyl phthalate, DNA Binding Proteins, MPF-2, not elsewhere classified, Other diseases of trachea and bronchus, avian, RESP COND: EXT AGENT NOS, DBP, l(3)84Ab, DABP, Other diseases of respiratory system NOS (disorder), BG:DS00004.13, Factor, increased time, Mycoplasmas and walled relatives, chronic, DISEASES OF THE RESPIRATORY SYSTEM, Cell, CHR PUL MANIF D/T RADIAT, dTAF230, prophase chromosome, 2-Benzenedicarboxylic acid dibutyl ester, DBP/GC, p230, Protein, high time, TAF[[II]]250/230, Respiratory system diseases NOS, TFIID, DNA Helix Destabilizing Proteins, prolonged period, Other diseases of mediastinum, Dibutyl o-phthalate, Taf[[II]]250, 2-dicarboxylate, Other alveolar and parietoalveolar pneumonopathy, Wall, TAF[[II]]230, HEL-S-51, underdeveloped, PNEUMOCONIOSES AND OTHER LUNG DISEASES DUE TO EXTERNAL AGENTS, Dibutyl 1, interphase chromosome, Single-Stranded, Disorder of respiratory system, Mfp-2, TAF[II]250, Disease of respiratory system (disorder), [X]Other diseases of the respiratory system, Mycoplasma gallisepticum, Gene Action Regulation, Respiratory conditions due to other and unspecified external agents, Chronic and other pulmonary manifestations due to radiation, PRLTS1, Dibutyl-o-phthalate, Respiratory disease, DmelCG17603, Disorder of respiratory system (disorder), MEDIASTINUM DISEASE NEC, 17-beta-HSD 4, 1.1.1.n12, response, Genome, TAF1Protein Gene Products, protoplasm, Gene Proteins, Infestation and Infection, data, nucleocytoplasm, protoplast, Protein, Gene Products, Proteins, Infection, Infestations and Infections, Gene, Infection and Infestation, proteins, associated, internal to cell, Infections and Infestations., intracellular, Mycoplasma gallisepticumfalseData on nucleoid-associated proteins isolated from Mycoplasma gallisepticum after intracellular infectionMycoplasma gallisepticum belongs to the class Mollicutes. It causes chronic respiratory disease in avian species. M. gallisepticum is characterized by lack of cell wall; reduced genome size and the volume of its nucleoid is comparable to the size of the whole cell. As a result of genome reduction, M. gallisepticum has a limited variety of DNA-binding proteins (DBP) and transcription factors. It was shown, however, that mycoplasmas demonstrate a wide range of differential expression in response to various stress factors, which promotes effective adaptation to unfavorable conditions. We assume that in the case of mycoplasmas, which are characterized by a combination of the reduction of known gene expression regulation systems and a high adaptive potential, the coordination of gene expression can be provided due to local changes in the structure and spatial organization of the chromosome. The study of the dynamic changes of the proteomic profile of M. gallisepticum nucleoid may assist in revealing its mechanisms of functioning, regulation of chromosome organization and stress adaptation including its changes upon invasion of the host 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