Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/WT-1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/WT-3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/Mut-3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/Mut-1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/WT-2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/Mut-2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/S.oneidensis_Results.mzid.gzftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/WT-1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/Mut-2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/Mut-1.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/WT-2.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/Mut-3.mgfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681/WT-3.mgfprimaryOK200virginie.brun@cea.frYohann CoutéMass SpectrometryShotgun proteomicsXcaliburMascotProlineRiboflavin productionNanolc-ms/msBiofilm formationShewanella oneidensisPutrescin decarboxylase spechttp://www.ebi.ac.uk/pride/archive/projects/PXD025681Proteomic analyses were conducted with cells that were inoculated in anoxic M4 minimal media with 70 mM lactate as electron donor and 100 mM fumarate as electron acceptor. The starting OD600 was 0.2. After 5 h of growth, cells were harvested by centrifugation (7 min, 6000 g, 4°C). The supernatant was discarded, and pellets were resuspended in TRIS buffer (pH 6.8). Cells were lysed by two passages through a French Press. Cell lysates were mixed with Laemmli buffer and then stacked in a single band in the top of a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel (4%–12% NuPAGE gel, Invitrogen, Illkirch-Grafenstaden/France). After staining with R-250 Coomassie Blue (Biorad, Schiltigheim/France), proteins were digested in gel using modified trypsin (sequencing grade, Promega, Charbonnières les Bains/France). Resulting peptides were analyzed by online nano-liquid chromatography coupled to tandem mass spectrometry (MS) (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific, Illkirch-Grafenstaden/France). For this purpose, peptides were sampled on a 300 μm × 5 mm PepMap C18 precolumn (Thermo Scientific, Illkirch- Grafenstaden/France) and separated on a 75 μm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ, 1.9 μm, Dr. Maisch, Ammerbuch-Entringen) using a 180-min gradient.PrideNot availableiodoacetamide derivatized residuemonohydroxylated residueacetylated residueThe MS and MS/MS data were collected by Xcalibur (Thermo Scientific, Illkirch-Grafenstaden/France). Peptides and proteins were identified by Mascot (Matrix Science, London/United Kingdom) through concomitant searches against the Uniprot database (S. oneidensis MR-1 taxonomy), a homemade classical contaminant database, and the corresponding reversed databases. Trypsin/P was chosen as the enzyme, and two missed cleavages were allowed. Precursor and fragment mass error tolerances were set at respectively at 10 and 25 mmu. Peptide modifications allowed during the search were carbamidomethyl (C, fixed), Acetyl (Protein N-term, variable) and oxidation (M, variable). The Proline software was used to filter the results with conservation of rank 1 peptides, peptide score ≥ 25, peptide length ≥ 7 amino acids, FDR of peptide-spectrum-match identifications < 1% as calculated on peptide spectrum-match scores by employing the reverse database strategy, and a minimum of 1 specific peptide per identified protein group. Proline was then used to perform a compilation, grouping and MS1 quantification of the validated protein groups.ProteomicsVirginie BrunQ Exactive HFEDyPCOMPLETEShewanella Oneidensis (strain Mr-1)yohann.coute@cea.fr34082787 Edel M, Sturm G, Sturm-Richter K, Wagner M, Ducassou JN, Couté Y, Horn H, Gescher J. Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis. Biotechnol Biofuels. 2021 14(1):130 10.1186/s13068-021-01981-3EDyPFrance10.6019/PXD025681<h4>Background</h4>Some microorganisms can respire with extracellular electron acceptors using an extended electron transport chain to the cell surface. This process can be applied in bioelectrochemical systems in which the organisms produce an electrical current by respiring with an anode as electron acceptor. These organisms apply flavin molecules as cofactors to facilitate one-electron transfer catalyzed by the terminal reductases and in some cases as endogenous electron shuttles.<h4>Results</h4>In the model organism Shewanella oneidensis, riboflavin production and excretion trigger a specific biofilm formation response that is initiated at a specific threshold concentration, similar to canonical quorum-sensing molecules. Riboflavin-mediated messaging is based on the overexpression of the gene encoding the putrescine decarboxylase speC which leads to posttranscriptional overproduction of proteins involved in biofilm formation. Using a model of growth-dependent riboflavin production under batch and biofilm growth conditions, the number of cells necessary to produce the threshold concentration per time was deduced. Furthermore, our results indicate that specific retention of riboflavin in the biofilm matrix leads to localized concentrations, which by far exceed the necessary threshold value.<h4>Conclusion</h4>This study describes a new quorum-sensing mechanism in S. oneidensis. Biofilm formation of S. oneidensis is induced by low concentrations of riboflavin resulting in an upregulation of the ornithine-decarboxylase speC. The results can be applied for the development of strains catalyzing increased current densities in bioelectrochemical systems.Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis.Edel Miriam M, Sturm Gunnar G, Sturm-Richter Katrin K, Wagner Michael M, Ducassou Julia Novion JN, Couté Yohann Y, Horn Harald H, Gescher Johannes Jdonneur d'une paire d'electrons, d230, Sodium Dodecyl, Esters, Tandem, postnatal development, Sodium Lauryl, Histological Labelings, Dodecyl Sulfate, Gene, dTAFII250, growth and development, beta-lactate, SDS polyacrylamide gel electrophoresis of proteins, EfW1, band, Mass Spectrometry Mass Spectrometry, CG7826, FUMARATE, Buffer, dmTAF[[II]]230, peptide, Polypeptides, Electrophoreses, peptido, dmTAF1, Taf230, Miquelon and St. Pierre, 2-hydroxypropanoate, B1, CG7835, Gene Products, CG42273, DGS, Histological, Fumaric Acid Ester, Min, base de Lewis, Separated, DmelCG42273, Pro-Mega, TAF250, Taf200, Divorced, electron donor, dTAF[[II]]250, Lewis-Base, Staining, MeCH(OH)CO2 anion, TFIID TAF250, SDS-PAGE electrophoresis, peptides, cel, cell, MS2, gel, beta-Trypsin, min, Fumaric Acid Esters, mAPC, Taf1p, Lauryl Sulfate, proteins, Mass Spectrometry, Divorces, Lewis base, AI047805, Miquelon and Saint Pierre, dTAF250, band cell, column, DmelCG1106, Acid Esters, D1, n, peptidos, Fumarate, TAF, 2-hydroxypropionate, lactate, Histological Labeling, PTHB1, fumarate, Sulfuric acid monododecyl ester sodium salt, DYRK1, dTAF[[II]]230, TAF[[II]]250, Proteins, Ester, Liquid Chromatography, Dmel_CG7826, TAF200, ion(1-), Acid Ester, l(3)84Ab, Labeling and Staining, BG:DS00004.13, Stainings, TAFII-250, TAF250/230, buffer, Labeling, Thermo Scientific, Cell, Peptide, Fumaric, dTAF230, development, polypeptide, Sodium Lauryl Sulfate, TAFII250, Dm1, C18, ME-IV., p230, Protein, stab cell, TAF[[II]]250/230, TFIID, Sodium, Dyrk1, Dmel_CG7835, tandem MS, Corsica, Mnb, MNB, AU020952, Taf[[II]]250, Separation, (2E)-but-2-enedioate, 10^[-9], band form, TAF[[II]]230, growth pattern, MS/MS, non-developmental growth, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, Separations, CC1, postnatal growth, Tripcellim, SDS polyacrylamide gel electrophoresis, rod neutrophil, b-lactate, TAF[II]250, ion(2-), Fumarate Esters, Mass Spectrometry-Mass Spectrometry, (E)-2-butenedioic acid, AW124434, CG17603, TAF[[II]], beta Trypsin, Saint Pierre and Miquelon, CG1106, ME-IV, Protein Gene Products, Irium, Gene Proteins, Trypure, Fumaric Acid, DmelCG17603, Labelings, Taf250, SR3-5, 2-hydroxypropanoic acid, Peptid, St. Pierre and Miquelon, Polypeptide, growth, Sulfate, TAF230, TAF1extracellular, Vitamin B2, Shewanella oneidensis Venkateswaran et al. 1999., Vitamin G, Vitamin B 2IPP2A2, RANK, Aminosaeure, Amino acid, TRANCER, protein, ter, 5730420M11Rik, peptide, Polypeptides, protein polypeptide chains, peptido, ClvPrd, MR-1, L-Proline, Software Engineering, protein aggregate, WMS, Computer Program, C79691, mRANK, SET, AI854243, amino acids, peptides, TAF-I, phosphatase 2A inhibitor I2PP2A, Open, Computer Programs and Programming, proteins, T6G21.3, KIPP1184, Lccp, DmelCG4299, set, IGAAD, DmelCG10574, Rank, Bsg75C, MNCb-5687, OPTB7, GPHYSD2, SGS, phapii, Stars, millidalton, Aminokarbonsaeure, 2210013N15Rik, StF-IT-1, FPD1, Thermo Scientific, Source Softwares, results, alpha-amino carboxylic acids, Software Tools, ACMICD, Programs, Program, Computer Applications, Term, Computer Applications Software, Computer Applications Softwares, TAHCCP2, Softwares, fixed, parent ion, Amino Acid, Acid, Software Applications, HLA-DR-associated protein II, Amino acids, DI-2, Source Software, PDC, I-2Dm, mmu, MASCOT, MASS, CG4299, inhibitor of granzyme A-activated DNase, Saint Pierre and Miquelon, I-2PP1, Tahccp2, Applications, TAF-IBETA, precursor ion, St. Pierre and Miquelon, TAF-Ibeta, Acids, Polypeptide, STARS, i2pp2a, Gruppe, Computer Software Applications, PDB2, Trans-activated by hepatitis C virus core protein 2, AW549739, Aminocarbonsaeure, FBN, Gene, precursor, Computer, alpha-amino acid, protein-containing complex, 3.-.-.-, PHAPII, Isle of Man, template-activating factor I, polypeptide chain, Ms1, ECTOL1, MR1, Miquelon and St. Pierre, Gene Products, Application, Open Source Softwares, ODFR, OSTS, MS1, TNFRSF11A, Ly109, Software Application, receptor activator of NF-KB, OFE, ipp2a2, Open Source Software, 2pp2a, Sciences, U.K., CG10574, Computer Software Application, OCTD, Miquelon and Saint Pierre, mKIAA0989, DmelCG4216, 2PP2A, Tools, taf-ibeta, grupos, male sterility 1, L Proline, dSET, dSet, FEO, peptidos, Applications Software, Open Source, data, Computer Software, grupo, milli unified atomic mass unit, protein complex, Proteins, igaad, Great Britain, alpha-amino acids, DYT8, backward, LOH18CR1, Peptide, Tool, group, polypeptide, Software Tool, TRANCE-R, native protein, natural protein, I-2PP2A, Protein, Dm I-2, 2810403H05Rik, I2PP2A, MFS1, Software, Corsica, Data Base, WMS2, CD265, mDa, MALE STERILITY 1 PROTEIN, Protein., Engineering, Striated muscle activator of Rho-dependent signaling, CG4216, Rest, Myofibrillogenesis regulator 1, Amino, osteoclast differentiation factor receptor, Protein Gene Products, Computer Programs, Gene Proteins, dSET/TAF-Ibeta, 2610030F17Rik, Applications Softwares, PKND1, BRP17, SSKS, Paroxysmal nonkinesiogenic dyskinesia protein, Brp17, Peptid, variable, AA407739, groupe, reversedsodium salt, 6-dinitrophenol, other pathways of electron transport, 6-phosphofructokinase reduction, anon-EST:fe2E2, d230, multi-cellular organism, l(3)alpha-Spec, cell-cell signaling involved in quorum sensing, determination, barium salt, alpha-Spect, e(-), alpha-Spe, Gene, lead salt, 4-dinitro-o-cresol, dTAFII250, electron, Spectrum Analyses, Vitamin G, EfW1, alpha-Spc, excreted substance, cell associated, Vitamin B 2, Quorum, dmTAF[[II]]230, dihydropteridine reduction, l(3)04276, potassium salt, B-spec, Quorum Quenchings, dmTAF1, Taf230, betaspec, responsivity, Quenching, klo, Gene Products, Mass, 2, 3, 4, excretion, alpha-Sp, Analysis, 6-dinitro-o-cresol, CG1977, animal, Mass Spectroscopy, alpha-spec, TAF250, Mass Spectrum Analysis, reactivity, Taf200, dTAF[[II]]250, kus, TFIID TAF250, Analyses, cel, cell, dihydrolipoylprotein reduction, lead(2+) salt, Elektron, dre3, beta-particle, Taf1p, proteins, alpha-spectrin, anon-EST:fe1B3, dTAF250, detection of cell density by secreted molecule, e, e-, SPEC8, species, CG5870, TAF, Shewanella oneidensis Venkateswaran et al. 1999, l(3)dre3, Spec-beta, 6-dinitro-ortho-cresol, quorum sensing system, dTAF[[II]]230, TAF[[II]]250, alpha-Spectrin, waste substance, Sensing, 3A9, body, DmelCG1977, protein-disulfide reduction, Proteins, TAF200, total expressed protein, electron transfer, portion of excreted substance, l(3)84Ab, whole body, BG:DS00004.13, TAFII-250, i168, TAF250/230, SpeC, Spectrum Analysis, L-ornithine carboxy-lyase activity, L-ornithine carboxy-lyase (putrescine-forming), Cell, dTAF230, Spectroscopy, organism, alphaSpec, TAFII250, l(1)G0108, p230, Protein, chemical analysis, Proteomes., 5-dinitro-2-hydroxytoluene, TAF[[II]]250/230, betaSpec, TFIID, 2-methyl-4, oxidized glutathione reduction, alphaSp, spectrin, b-Spec, Mass Spectrum Analyses, Quorum Quenching, Quenchings, Taf[[II]]250, Vitamin B2, Mass Spectrum, ammonium salt, DmelCG5870, l(1)G0074, l(1)G0198, TAF[[II]]230, whole organism, negatron, flavin, DNOC cpd, FCP-B, Spectrometry, b spectrin, INSDC_feature:gene, TAF[II]250, alpha, hedolit, CG17603, TAF[[II]], beta, beta(-), Protein Gene Products, extracellular, Gene Proteins, DmelCG17603, Taf250, concentration, l(3)62Bd, beta-spec, dihydrolipoamide reduction, SR3-5, i6, Koerper, alfa-Spec, assay, response, cell bound, 6-dinitro-2-cresol, dihydrobiopterin reduction, Spec, Specbeta, TAF230, DNOC, TAF1projections, sodium salt, other pathways of electron transport, single-organism developmental process, postnatal development, alpha-Spe, lead salt, 4-dinitro-o-cresol, growth and development, increased number., (DL)-Isomer, alpha-Spc, Long Term, cell associated, DmelCG6383, dihydropteridine reduction, l(3)04276, responsivity, 5-Diaminopentanoic acid, 1, 2, 3, 4, excretion, animal, Effect, alpha-spec, increased, me75, kus, dihydrolipoylprotein reduction, ornithine, crumb, beta-particle, proteins, number of, D17Mit170, alpha-spectrin, T1, Ornithine Monohydrobromide, retention, e, e-, papilla, Strains, 4-Diaminobutane, Long-Term Effects, Shewanella oneidensis Venkateswaran et al. 1999, anatomical protrusion, CG6383, lamina, Longterm Effect, flanges, electron transfer, Ornithine Monoacetate, extra or missing physical or functional parts, Tl3, Tl2, results, L-ornithine carboxy-lyase activity, Ornithine Dihydrochloride, organism, alphaSpec, l(1)G0108, batch, shelf, 5-Diaminovaleric acid, Ornithine Phosphate (1:1), 2-methyl-4, Biofilm, oxidized glutathione reduction, spectrin, Ornithine, Vitamin B2, ammonium salt, whole organism, maintenance of localization, growth pattern, Orn, non-developmental growth, shelves, DNOC cpd, INSDC_feature:gene, alpha, hedolit, projection, ridge, beta(-), (D)-Isomer, 4-Butanediamine, spine, dihydrolipoamide reduction, i6, Koerper, (L)-Isomer, 5-diaminopentanoic acid, cell bound, Strains and Sprains, time, l(3)S050920, dihydrobiopterin reduction, Specbeta, accessory, 5 Diaminopentanoic Acid, 6-dinitrophenol, 6-phosphofructokinase reduction, Crbs, Ornithine Sulfate (1:1), anon-EST:fe2E2, multi-cellular organism, localised, l(3)alpha-Spec, Effects, lamellae, Tetramethylenediamine, barium salt, alpha-Spect, e(-), number, Gene, far, electron, Vitamin G, process of organ, supernumerary, excreted substance, Vitamin B 2, protrusion, lamella, period, potassium salt, B-spec, Crumbs, betaspec, klo, Gene Products, alpha-Sp, Low, 6-dinitro-o-cresol, CG1977, Sprains, reactivity, study, CT19912, 0509/20, Longterm, lead(2+) salt, Elektron, 1384/04, dre3, ridges, Long-Term, anon-EST:fe1B3, SPEC8, has or lacks parts of type, Long-Term Effect, species, CG5870, laminae, l(3)dre3, l(3)j1B5, Spec-beta, 6-dinitro-ortho-cresol, alpha-Spectrin, cou, waste substance, 3A9, body, DmelCG1977, anatomical process, protein-disulfide reduction, Proteins, Sprain, portion of excreted substance, whole body, i168, SpeC, L-ornithine carboxy-lyase (putrescine-forming), Cell, l(3)07207, mereological quality, development, 5-Diaminopentanoic Acid, Lr, DL-Ornithine, Ornithine Hydrochloride, 4 Diaminobutane, Protein, Long Term Effects, Strain, 5-dinitro-2-hydroxytoluene, 4 Butanediamine, betaSpec, CRB, Crb, focal, background, alphaSp, l(3)S058104, b-Spec, flange, organ process, DmelCG5870, l(1)G0074, l(1)G0198, negatron, storage, flavin, postnatal growth, FCP-B, b spectrin, Ornithine Monohydrochloride, introduction, beta, Longterm Effects, Protein Gene Products, extracellular, processes, process, Gene Proteins, present in greater numbers in organism, concentration, l(3)62Bd, beta-spec, cardinality, Bra, processus, alfa-Spec, response, sequestering, growth, 6-dinitro-2-cresol, Spec, DNOCSpec-beta, DmelCG5870, l(1)G0074, anon-EST:fe2E2, alpha-Spectrin, l(1)G0198, l(3)alpha-Spec, kus, 3A9, DmelCG1977, alpha-Spect, FCP-B, alpha-Spe, dre3, b spectrin, alpha, i168, alpha-Spc, SpeC, alpha-spectrin, L-ornithine carboxy-lyase activity, L-ornithine carboxy-lyase (putrescine-forming), beta, anon-EST:fe1B3, alphaSpec, l(3)04276, l(1)G0108, B-spec, l(3)62Bd, beta-spec, SPEC8, betaspec, i6, proteomic analysis, klo, alfa-Spec, betaSpec, alpha-Sp, CG5870, CG1977, alphaSp, alpha-spec., spectrin, b-Spec, Shewanella oneidensis Venkateswaran et al. 1999, Spec, Specbeta, l(3)dre3falseProteomic analysis of Shewanella oneidensis overexpressing or not the putrescin decarboxylase speCSome microorganisms can respire with extracellular electron acceptors using an extended electron transport chain to the cell surface. These organisms apply flavin molecules as cofactors to facilitate one-electron transfer catalysed by the terminal reductases and as endogenous electron shuttles. In the model organism Shewanella oneidensis, riboflavin production and excretion triggers a specific biofilm formation response that is initiated at a specific threshold concentration, similar to canonical quorum sensing molecules. Riboflavin-mediated messaging is based on the overexpression of the gene encoding the putrescin decarboxylase speC which leads to posttranscriptional overproduction of proteins involved in biofilm formation. We performed a mass spectrometry-based analysis of cells with and without speC overexpression to identify the effect of SpeC overexpression on the cell proteome.2021-07-162021-04-28PXD02568128065516460991804982953585111454224942024527872842188090368093270429330845811363336351524260119834300662758385NCBITaxon:128068888561896111430118726355557114526236493461364031952065126957366649274670738538836411354615821582310665688954762721123294422291180992323122586365458333460792519365914320597129386558195851149315242592637371250232NCBITaxon:1272111957748186614885843104300245135867221755437408169180454266834295319453058612668351498225058149636745855452811320814795641003923751463694204943320896420896344597413797912838440066793529367156759388036478434716052165994048321659721659545501315282693042165915627161696833218321729204277944762376454317203094980197525556978159509439843948840479NCBITaxon:2157525338551531051067439899037122857759515205968762262311210884555588845587077918178081736309431794571698936160488409859NCBITaxon:37162104782679401505907148364320421061320491381693630355543204663263331199456545351102541110321745921612928867641002642036945694101676341021076127735113165823925472793342814613584301417298174633457744491675476048932128735675684030135062191726518130194111929439367110411310239320255598632582788396591047593474951604901652998355256631758543980110243260866310245980875741755518320196447145268045796631367019083233115611415535510421932555217634981812359871069955529NCBITaxon:4751850298335476113615741465477180066556484337677299767982510216998238976484074137351155711089725482444474153NCBITaxon:5055789969844100036465349334147147493548019467512991143597412764731370863265478765698353221599838192011106775772710348198523487804794640986099586157187176685991915729513722117110129292107687834974198602028091488406334249234235152335985988655974649987039202111072943311711397215987419085037117082711708284182350241451139692931902197822474636046147123749402375939723287581722532566489050794526591972211915853101774208588596NCBITaxon:615750521028307989515586411051989972375059576131720002634196102641088878176179212190659407121906772275885815689964975151950582873757244NCBITaxon:22023717NCBITaxon:17036122019998570506174767927450627249159615333554184005246432136861505964878486662941786661347515363291018194301086731018510184NCBITaxon:1763573039635586331492862876705801321650852718481865368595105135553482260710185687294296110091416333NCBITaxon:619184023210007348824123363630710470058127187878645159812894555663577553470951451225786260704NCBITaxon:13356262607052607076584574054163677483167233150561007133705654195NCBITaxon:305550445791399878168119011928752829126806315327341478281055524NCBITaxon:9606135858469008111167815022830519302836728593008529005NCBITaxon:56938268830640179469915101184994352581515901448242838111394426041937047592723708604232816772850351581990119519532673863701370249240536088385816224881861568175626158355837153337605726711935019980861193502167879108579137113712724071906503714759172629815712562745757270903113335503728142433747924910078711082NCBITaxon:96157243359463318586260799141262200298492670NCBITaxon:126371928124921252830385683856959116926610065812375612750454266625515197881356638375064422336009412934979054632245352472140125751511649908515151438992374537474454464152432359376059202228870515805955365550881579327160293851578114978633892894535936479437NCBITaxon:6854412754225117159010901341673871589552101296610306129671585158451584937659393432715910022637673388990461423451287689591198332966467041464792936576164280341160221641331599280382803536909108917688149894621812141080348179392287889295027113133295282829445686174934681911265269972273218903821076012471904007271597369978281143114020641198108713554774365814245072295337969820423212422312916321803347087145372091645733215358157546992877702020603281143193382935508332912922114872526499972830357390871830872672710379085507176299707678926837041557372921405329412816078395639243142626548982273775962073220865958827355516936053307972551811354915974953502611595561432138406072587814144715094933491949634016143347280059360463164610466038151845474485003567097477771951240174841855601335755547920427460769946881037620148871005941289093164354282688500712303831389464940838283594144242322197857742171598921409212205853939621157781154756929793244704749906604162103591905158114452749907312987779913112273170187546991103589915NCBITaxon:3729610360983341871049113079813367953164072994671034015786582645303337330638301130641399253064236365059116965478854698112116203431710337286129940445862073401153571119529652611745531114716133865413131663430552132312017983602532373127612552329935129938505731631764261979401151345844577267872862321933415134618620862116079139222798351681196125522814953932232311676175731210807721348799994886185696281408434103351996321849620994887321392488209711533820961153392095330439375569927612686266447NCBITaxon:269704933986243694110312195883205092029061013068599975864445882864324995252972464260667411583432127178942786378942614774052567378639123343551419983493179986865540553435291486545147124546688925104298635712467915164135825763288401937334508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