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Biofilm matrix and total proteins were extracted as previously described (Sugimoto et al. (2013) Journal of bacteriology 195, 1645-1655) with little modification. Briefly, biofilms were collected from the bottom of the dish and washed with washing buffer composed of 10 mM Tris-HCl (pH 8.0) and protease inhibitor cocktail (Sigma-Aldrich) followed by centrifugation at 5,000 g for 10 min. The pellet was dissolved in a matrix extraction buffer composed of 10 mM Tris-HCl (pH 8.0), 1 M NaCl, and protease inhibitor cocktail followed by incubation at 25°C for 30 min with gentle rotation. The mixture was centrifuged at 5,000 g for 10 min after incubation and the supernatant was collected as the biofilm matrix protein. For extracting the total protein, the pellet was lysed by B-PER™ bacterial protein extraction reagent (Thermo Scientific) according to the manufacturer’s instructions and sonicated with a Q125 Sonicator (Qsonica) set at 65% amplitude (five blasts each lasting 15s with 30-s pauses). The supernatant was collected as the total protein after centrifugation. For all proteomics experiments, three biological replicates were performed for each sample including the control sample.  Collected proteins were desalted with Thermo Pierce C18 spin tips and digested with trypsin (Pierce™ Trypsin Protease, MS Grade) before injecting into the Bruker timsTOF Pro Mass-spectrometer (Bruker Headquarters Billerica, Massachusetts, US) with Capitve Spray Ion source. Approximately 200 ng of the digested protein was injected into the Bruker nanoElute system and separated on a C18 column (ionoptiks Aurora UPLC column, Part no. AUR2-25075C18A-CSI) that was eluted with a 30 min gradient of 2–95% aqueous acetonitrile containing 0.1% formic acid at a flow rate of 0.3 μL/min. The m/z range acquired in the MS full scan was 100 to 1700 Da."],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["The generated raw data were converted to mgf files by Bruker Compass DataAnalysis, and subsequently converted to mzML files by msconvert of the ProteoWizard. The mzML files were searched using Comet (version 2016.01 rev.2) with a custom database. Briefly, the genome sequence of MRSA ATCC 43300 was converted into a protein database using GeneMark gene prediction tool. The proteins were then annotated using BLASTp from NCBI using MRSA NCTC 8325 as the protein database. The sequences of common contaminants, such as trypsin and human keratins, and decoy sequences generated by shuffling amino acid sequences between tryptic cleavage sites were added to the database. The decoy sequences in the database are used for the false discovery rate (FDR) estimation of the identified peptides. The search parameters criteria were set as follows: 15 ppm peptide mass tolerance, monoisotopic mass type, fully digested enzyme termini, 0.05 amu fragment bin tolerance, 0 amu fragment bin offset, carbamidomethylated cysteine and oxidated methionine as the fixed and variable modifications respectively. The search results from Comet were processed by PeptideProphet, iProphet and ProteinProphet of the Trans-Proteomics Pipeline (TPP) in the decoy-assisted non-parametric mode. Every mzML run was analyzed independently. Protein identifications were filtered at a false discovery rate of 0.01 as predicted by ProteinProphet."],"omics_type":["Proteomics"],"labhead":["Henry Lam"],"instrument_platform":[""],"labhead_affiliation":["Chemical and Biological Engineering Department, The Hong Kong University of Science and Technology (HKUST)"],"submission_type":["PARTIAL"],"species":["Staphylococcus Aureus"],"publication":["Long L, Sulaiman JE, Xiao Y, Cheng A, Wang R, Malit JJ, Wong WC, Liu W, Li YX, Chen F, Lam H, Qian PY. Mode of action of elasnin as biofilm formation eradicator of methicillin-resistant <i>Staphylococcus aureus</i>. Front Microbiol. 2022 13:967845 10.3389/FMICB.2022.967845"],"submitter_mail":["jesulaiman@connect.ust.hk"],"submitter_affiliation":["The Hong Kong University of Science and Technology (HKUST)"],"submitter_country":["Hong Kong"],"sample_synonyms":["sodium salt, DIWS, Ghrfr, IPP2A2, Endopeptidase Inhibitor, ammonium formate, 13C-labeled, MeCN, cadmium salt, NCMe, determination, classic hairy cell leukaemia, Peptide Hydrolase Inhibitor, zinc formate, Gene Expression Profile, lead salt, Profiles, Hydrogenchlorid, protein, magnesium formate, composed of, ankylosing vertebral hyperostosis with tylosis, zinc salt, BLAST, prevention, l(2)k09905, 5730420M11Rik, halite, ammonium tetraformate, hydrogen chloride, Biofilm Matrices, cobalt(II) formate dihydrate, Protease Inhibitor, CH3-C#N, B1, F17H15_9, 3, Inhibitors, Min, Williams-Beuren syndrome chromosomal region 6 protein, Hydrolase Inhibitors, protein aggregate, prevention and control, WMS, DmelCG42273, F17H15.9, DmelCG8428, SET, 5-dibutyl-6-(1-butyl-2-oxoheptyl)-4-hydroxy-, Spin, Divorced, Extracellular Polymeric Substances, reference sample, TAF-I, min, composition, mAPC, Divorces, copper, Signatures, AI047805, DmelCG4299, preventive measures, cloruro sodico, salt, IGAAD, set, SPIN, Wasserstoffchlorid, Peptidohydrolase Inhibitor, lithium salt, Bacterial Gene Proteins, column, DmelCG10574, Expression Signature, BTKAP1, ataurora2, Diws1t, sample, D1, Rotations, Transcriptomes, Reagents, GPHYSD2, GTFII-I, ammonium (2:1) salt, reagents, SGS, nickel salt, 6030441I21Rik, phapii, EPS Matrices, rock salt, preventive therapy, Peptidase Inhibitor, cobalt (+2) salt, Expression Profiles, Dmel_CG7826, StF-IT-1, Th, lit, classic hairy cell leukemia, Peptide Peptidohydrolase, BAP135, Thermo Scientific, Exopolymers, Forestier disease, ACMICD, Gene Expression, Extracellular Polymeric Substance, common salt, sodium (4:1:1) salt, Dm1, magnesium salt, Expression Signatures, Hydrolase Inhibitor, Chlorwasserstoff, SRF-Phox1-interacting protein, BAP-135, Biofilm, reactif, nickel (+2) salt, Expression Profile, Dmel_CG7835, Transcriptome Profiles, little, Mnb, MNB, Clinorotations, l(2R)W5, ammonium salt, HLA-DR-associated protein II, Antagonists, DI-2, Xylella fastidiosa str. Temecula1, content, I-2Dm, HCl, formate, cobaltous formate, reagent, cromium (+3), Bacterial Gene Protein, CG4299, MASS, AW124434, GtfII-I, natrii chloridum, beta Trypsin, Polymeric Substance, I-2PP1, Clinorotation, Peptidase Inhibitors, TAF-IBETA, l(2)W5, WBS, Indicator, copper salt, Antiproteases, ammonium (4:1) salt, CG8428, TAF-Ibeta, Endopeptidase, Peptide Peptidohydrolase Inhibitor, methyl cyanide, chlorane, lead (+2) salt, Inhibitor, i2pp2a, nickel formate dihydrate, EPS, not genetically inherited., lead formate, Transcriptome Profile, Peptidomics, chlorure d'hydrogene, Endopeptidase Inhibitors, Reagent, EPS Matrix, Bacterial Protein, aluminum salt, cesium salt, Pierce, Matrix, FBN, Gene, Protease, protein-containing complex, thomson, Gene Product, TFII-I, CG7826, PHAPII, Buffer, Bruton tyrosine kinase-associated protein 135, formic acid, mass-to-charge ratio, Blasts, potassium salt, ECTOL1, 14C-labeled, Gene Products, CG7835, CG42273, IB291, Protease Antagonist, Peptide Hydrolase, AA673430, Separated, cloruro de hidrogeno, Reagents and Indicators, anatomical systems, reactivo, Hydrogen chloride, AtAUR2, thallium (+1) salt, Profile, ipp2a2, beta-Trypsin, rubidium salt, chlorure de sodium, 2pp2a, calcium formate, methanoic acid, Antagonist, Indicators, CG10574, Xylella fastidiosa (strain Temecula1 / ATCC 700964), OCTD, 2PP2A, Protease Antagonists, taf-ibeta, Extracellular, Kochsalz, dSET, dSet, Peptide Peptidohydrolase Inhibitors, cromium (+3) salt, AA553322, BTK-associated protein 135, Gene Protein, sodium formate, PTHB1, Controlled, WBSCR6, Controlling, DYRK1, Transcriptome, 1-(14)C-labeled, nickel formate, 3H-labeled, protein complex, Proteins, igaad, strontium formate, chloridohydrogen, potassium formate, Mischung, copper (+2) salt, compositionality, acetonitrile, strontium salt, buffer, Peptide, group, bnch, whole transcriptome, Bacterial Gene Product, l(2)k02511, [HCl], native protein, Natriumchlorid, I-2PP2A, C18, cupric formate, Gene Expression Signatures, chemical analysis, Protein, Dm I-2, I2PP2A, Hydrochloride, table salt, MFS1, Gene Expression Signature, Dyrk1, 25/11, NaCl, aluminum formate, ACETONITRILE, Biofilm Matrix, Bacterial Gene Products, WMS2, cyanomethane, AU020952, Bacterial, Exopolymer, Separation, lithium formate, PRSS, ensemble, prophylaxis, Separations, CC1, Tripcellim, ethanenitrile, Peptidohydrolase Inhibitors, sample population, chromic formate, ME-IV, Protein Gene Products, l(2)10403, Gene Proteins, Trypure, Peptidase, dSET/TAF-Ibeta, 2610030F17Rik, Antiprotease, control, Gene Expression Profiles, structure, SSKS, 2H-Pyran-2-one, calcium salt, assay, Peptide Hydrolase Inhibitors, Signature, AA407739, hydrochloric acid"],"name_synonyms":["MRSA bacteremias, Therapy, treatment, Methicillin-resistant Staphylococcus aureus (MRSA) infection, 5-dibutyl-6-(1-butyl-2-oxoheptyl)-4-hydroxy-, disease management., infection by MRSA, methicillin-resistant Staphylococcus aureus (MSSA) bacteremia, Therapeutic, Peptidomics, NHE6, methicillin-resistant staphylococcus aureus infectious disease, Methicillin Resistant Staphylococcus aureus, Infection, 2H-Pyran-2-one, Therapies, MRSA infection, MRSA bacteremia, 3, Treatment, Methicillin-resistant Staphylococcus aureus, MRSA, Biofilm, Treatments"],"data_synonyms":["MGC130048, Bru, IPP2A2, Materials, E 920, Raw, Aminosaeure, leg, Somatomedin-C, MRSA bacteremia, cleavage, MUP24_16, Keratin-Associated Proteins, Amino acid, A4, protein, contrasted, MUP24.16, 5730420M11Rik, l(1)AA33, peptide, Polypeptides, L-Isomer Methionine, Methionine, peptido, methicillin-resistant Staphylococcus aureus (MSSA) bacteremia, Keratin Associated, 1, 2, 2-amino-3-mercaptopropanoic acid, Cystein, Rev3, protein aggregate, WMS, Svc, gamma sarcoglycan, SET, C, amino acids, Man (Taxonomy), peptides, Genomes, TAF-I, cisteina, M, SF, Kitl, Mast cell growth factor, Keratin Associated Proteins, alpha-Keratin, DmelCG4299, HOMEODOMAIN-LEUCINE ZIPPER PROTEIN IFL1, IGAAD, set, DmelCG10574, l(1)B2/13.1, RUN, Run, ppm, Mechano growth factor, gamma-sarcoglycan, tripeptidyl-peptidase II activity, Soluble KIT ligand, u, Half Cystine, Sl, GPHYSD2, Racemethionine, SGS, phapii, IGF-I, Rev, l(1)LB9, Zinc Cysteinate, REVOLUTA, T5E21.12, Aminokarbonsaeure, Modern, Keratin, SG-gamma, 2-Amino-4-(methylthio)butyric acid, StF-IT-1, unified atomic mass unit, MRSA, IBP1, Cys, alpha-amino carboxylic acids, Da, results, sKITLG, AA33, ACMICD, infection by MRSA, Methicillin Resistant Staphylococcus aureus, MRSA infection, Genetic Materials, sarcoglycan, Methionin, Methicillin-resistant Staphylococcus aureus, Hmet, Igf-1, (2R)-2-amino-3-sulfanylpropanoic acid, fixed, Genetic Material, T5E21_12, predicted., Amino Acid, Acid, SHEP7, HLA-DR-associated protein II, mast cell growth factor, Amino acids, DI-2, Sez4, IFL, I-2Dm, Stem cell factor, common, INSDC_feature:gene, L-Cysteine, whole genome, CG4299, MASS, STAT5, gamma (35kDa dystrophin-associated glycoprotein), beta Trypsin, human, alpha Keratin, L-Cystein, I-2PP1, Kitlg, KL-1, DMDA, TAF-IBETA, Material, 35kD dystrophin-associated glycoprotein, methicillin-resistant staphylococcus aureus infectious disease, Biocatalyst, Cistron, Acids, TAF-Ibeta, Polypeptide, i2pp2a, Liquimeth, Gb, Cytokeratin, CG1849, Clo, human being, Cysteine Hydrochloride, 2-amino-4-(methylsulfanyl)butanoic acid, SGCG_HUMAN, Peptidomics, Slf, SLF, Biocatalysts, Aminocarbonsaeure, L-Zystein, L Isomer, FBN, Gene, FPH2, alpha-amino acid, protein-containing complex, TYPE, PHAPII, Human, DAGA4, Homo sapiens, ECTOL1, 35DAG, Gene Products, L-Methionine, Del(8)44H, Half-Cystine, MAM, gamma-SG, Keratin Associated Protein, SCG3, INTERFASCICULAR FIBERLESS 1, Man, Pedameth, InterProphet, Genetic, L Cysteine, E-920, Runt, ipp2a2, beta-Trypsin, 2pp2a, TPP, Hcys, CG10574, IFL1, OCTD, Con, Enzyme, 2PP2A, 2-amino-4-(methylthio)butanoic acid, 10^[-6], CYSTEINE, taf-ibeta, dSET, dSet, blz, FREE CYSTEINE, peptidos, INTERFASCICULAR FIBERLESS, Associated Protein, (2R)-2-amino-3-mercaptopropanoic acid, metionina, LB5, 35 kDa dystrophin-associated glycoprotein, DL-Methionine, protein complex, Proteins, igaad, DmelCG1849, alpha-amino acids, Rnt, L-Isomer, Cistrons, Peptide, SGCG, group, LGMD2C, lLB5, polypeptide, native protein, I-2PP2A, Protein, Dm I-2, I2PP2A, Infection, sequence, MFS1, Zystein, Data Base, WMS2, Met, MRSA bacteremias, Methicillin-resistant Staphylococcus aureus (MRSA) infection, Col4a-1, PRSS, amu, DMDA1, ensemble, 2-amino-3-sulfanylpropanoic acid, Tripcellim, L-2-Amino-3-mercaptopropionic acid, (R)-2-amino-3-mercaptopropanoic acid, l(1)19Ea, Amino, primary structure of sequence macromolecule, 2-Amino-3-mercaptopropionic acid, Protein Gene Products, P235, Gene Proteins, Trypure, dSET/TAF-Ibeta, 2610030F17Rik, HOMEODOMAIN-LEUCINE ZIPPER PROTEIN, MGF, Mgf, Modern Man, c-Kit ligand, SSKS, SCARMD2, NHE6, alpha-amino-gamma-methylmercaptobutyric acid, E920, Peptid, SCF, variable, AA407739"],"description_synonyms":["PhrB photolyase activity, Peptidomics, P62, MRSA bacteremia, sci, photoreactivating enzyme activity, pigmented epithelium, methicillin-resistant Staphylococcus aureus (MSSA) bacteremia, isolation, Biological, HOW, How, 3, Cell., stratum pigmentosa retinae, NUP96, epithelium, l(3)j5D5, INSL3R, 24B, treatment, study, 5-dibutyl-6-(1-butyl-2-oxoheptyl)-4-hydroxy-, MOS3, Biologic Assays, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, Gpr106, l(3)s2612, PRECOCIOUS, High G+C Gram-Positive Bacteria, Assays, pigmented retina, stru, F23A5.3, RXFPR2, purification, l(3)S053606, CG10293, DNA cyclobutane dipyrimidine photolyase activity, SUPPRESSOR OF AUXIN RESISTANCE 3, isolation and purification, l(3)j5B5, GREAT, Actinomycete, Gram-Positive Bacteria, DmelCG10293, disease management, Great, Therapies, GPR106, F23A5_3, Biologic, Therapy, PRE, 0904/17, Actinobacteriota, clone 2.39, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, Assay, MRSA, qkr, l(3)S090417, Cell, actinobacteria, MODIFIER OF SNC1, infection by MRSA, retinal pigment, SZ1, KH93F, deoxyribonucleic photolyase activity, Methicillin Resistant Staphylococcus aureus, Infection, MRSA infection, dipyrimidine photolyase (photosensitive), Methicillin-resistant Staphylococcus aureus, Biofilm, Biologic Assay, retinal pigment layer, who, MRSA bacteremias, LGR8, Methicillin-resistant Staphylococcus aureus (MRSA) infection, Lgr8, Actinobacteraeota, RPE, photolyase activity, Who/How, Actinobacteria, LGR8.1, Treatments, p. pigmentosa retinae, Biological Assays, Bioassay, phr A photolyase activity, Bioassays, DNA-photoreactivating enzyme, bioassay, Therapeutic, High G+C, NHE6, methicillin-resistant staphylococcus aureus infectious disease, 2H-Pyran-2-one, qkr[93F], anon-EST:Liang-2.39, Treatment, deoxyribonucleate pyrimidine dimer lyase (photosensitive), Actinomycetes"],"additional_accession":[]},"is_claimable":false,"name":"Proteomics of MRSA biofilms under Elasnin Treatment","description":"Due to the great challenge in treating biofilms, there is an urgent need for novel and effective antibiofilm compounds. Through bioassay-guided isolation of bioactive compounds from Actinobacteria, we identified elasnin as a potent biofilm-targeting compound against methicillin-resistant Staphylococcus aureus (MRSA). Elasnin effectively inhibited biofilm formation and eradicated pre-formed biofilms of MRSA. To gain more insights on how elasnin eradicate MRSA biofilms, we conducted proteomics study on MRSA biofilm cells under elasnin treatment compared to the untreated cells.","dates":{"publication":"2022-07-22","submission":"2021-06-21"},"accession":"PXD026836","cross_references":{"TAXONOMY":["NEWT:3555","NEWT:71647","NEWT:35554","NEWT:32046","NEWT:544496","NEWT:2042546","NEWT:32049","NEWT:1392696","NEWT:259447","NEWT:45351","NEWT:445974","NEWT:43179","NEWT:180454","NEWT:5722","NCBITaxon:1280","NEWT:1129","NEWT:309807","NEWT:55153","NEWT:309800","NEWT:281395","NEWT:1211601","NEWT:876138","NEWT:44271","NEWT:10036","NEWT:1590","NEWT:498019","NEWT:1351","NEWT:1438992","NEWT:1352","NEWT:2649997","NEWT:1147161","NEWT:263737","NEWT:224326","NEWT:1333499","NCBITaxon:79857","NEWT:1096976","NEWT:95648","NEWT:1589","NEWT:135622","NEWT:1349","NEWT:96731","NEWT:383379","NEWT:10029","NEWT:913645","NEWT:44491","NEWT:556484","NEWT:317447","NEWT:4688","NEWT:7955","NEWT:7959","NEWT:2261","NEWT:31156","NEWT:3112","NEWT:4442","NEWT:192875","NEWT:1214915","NEWT:12637","NEWT:59729","NEWT:2164133","NEWT:108061","NEWT:1316931","NEWT:224308","NEWT:3347","NEWT:511145","NEWT:931281","NEWT:658457","NEWT:1310161","NEWT:77133","NEWT:295358","NEWT:145481","NCBITaxon:79824","NEWT:2246","NCBITaxon:4563","NEWT:5755","NEWT:44689","NEWT:44447","NEWT:498211","NEWT:5759","NEWT:1736231","NEWT:5518","NEWT:398007","NEWT:1392","NEWT:498217","NEWT:2242","NEWT:11320","NEWT:286","NEWT:391619","NEWT:246196","NEWT:287","NEWT:246197","NEWT:633149","NEWT:10239","NEWT:44685","NEWT:161934","NEWT:1148","NEWT:5508","NEWT:5507","NEWT:410661","NEWT:55571","NEWT:35500","NEWT:1140","NCBITaxon:2157","NEWT:1143","NEWT:4896","NEWT:1390","NEWT:11557","NEWT:1094343","NEWT:1336795","NEWT:644042","NEWT:294","NEWT:1773","NEWT:1182590","NEWT:3712","NEWT:82380","NEWT:935293","NEWT:375146","NEWT:118698","NEWT:1616117","NEWT:263","NEWT:118696","NEWT:52283","NEWT:284812","NEWT:8175","NEWT:43330","NEWT:1603293","NEWT:408169","NEWT:44664","NEWT:47946","NEWT:3702","NEWT:243277","NEWT:7067","NEWT:118694","NEWT:2850","NEWT:118691","NEWT:33548","NEWT:274","NEWT:408172","NEWT:408170","NEWT:96794","NEWT:3708","NEWT:332648","NEWT:44670","NEWT:536231","NEWT:376219","NEWT:1436733","NEWT:460519","NEWT:1247411","NEWT:572307","NEWT:1432138","NEWT:1424507","NEWT:1194599","NEWT:272844","NEWT:9483","NEWT:333760","NEWT:1513458","NCBITaxon:40559","NEWT:506599","NEWT:84588","NEWT:1679718","NEWT:480","NEWT:67767","NEWT:46835","NEWT:109757","NEWT:582580","NEWT:300852","NEWT:1502","NEWT:376686","NEWT:95486","NEWT:508771","NEWT:1883446","NEWT:253","NCBITaxon:2759","NEWT:1233435","NEWT:93061","NEWT:93062","NCBITaxon:4932","NEWT:644223","NEWT:235443","NEWT:108458","NEWT:272623","NEWT:272624","NEWT:320637","NEWT:983964","NEWT:118499","NEWT:32644","NEWT:527796","NEWT:499175","NEWT:109779","NEWT:3745","NEWT:1715989","NCBITaxon:4751","NEWT:476272","NEWT:3747","NEWT:195051","NEWT:3988","NEWT:1255228","NEWT:649908","NEWT:410289","NEWT:373153","NEWT:3983","NEWT:352472","NEWT:1071661","NEWT:360094","NEWT:470","NEWT:41364","NEWT:1313","NEWT:39491","NCBITaxon:5811","NEWT:29491","NEWT:2014887","NEWT:33952","NEWT:261756","NEWT:571256","NEWT:40483","NEWT:272634","NEWT:9031","NEWT:1872122","NEWT:7091","NEWT:141262","NEWT:108931","NEWT:1055524","NEWT:4087","NEWT:9778","NEWT:306","NEWT:150475","NEWT:303","NEWT:267872","NEWT:7111","NEWT:347515","NEWT:9534","NEWT:5180","NEWT:256737","NEWT:4090","NEWT:4092","NEWT:9541","NEWT:8694","NEWT:4093","NEWT:4096","NEWT:8692","NEWT:185579","NEWT:941442","NEWT:13076","NEWT:1226408","NEWT:7108","NEWT:40479","NEWT:4076","NEWT:317","NEWT:1006581","NEWT:885318","NEWT:39488","NEWT:550","NEWT:4081","NEWT:273068","NEWT:554","NEWT:98334","NEWT:451516","NEWT:4084","NEWT:63577","NEWT:36185","NEWT:1225786","NEWT:7574","NEWT:1715256","NEWT:30640","NEWT:575412","NEWT:929793","NEWT:29204","NEWT:28112","NEWT:114155","NEWT:6494","NEWT:6491","NEWT:507601","NEWT:186441","NEWT:643680","NEWT:214092","NCBITaxon:6157","NEWT:6239","NEWT:162425","NEWT:216257","NEWT:102169","NEWT:9986","NEWT:4054","NEWT:8654","NEWT:8658","NEWT:1268063","NEWT:8655","NEWT:1263854","NEWT:118503","NEWT:2059687","NEWT:160488","NEWT:28104","NEWT:407821","NCBITaxon:2","NEWT:985076","NEWT:1215323","NEWT:986","NEWT:52641","NEWT:7159","NEWT:28532","NEWT:353152","NEWT:8496","NEWT:27448","NEWT:40674","NEWT:1194669","NEWT:51329","NEWT:243230","NEWT:1080772","NEWT:519","NEWT:8479","NEWT:8485","NEWT:6063","NEWT:49240","NEWT:104395","NEWT:6289","NEWT:6287","NEWT:300641","NEWT:727","NEWT:9796","NEWT:725","NEWT:469008","NEWT:256318","NEWT:9321","NEWT:206411","NCBITaxon:6191","NEWT:596153","NEWT:229533","NEWT:1836","NEWT:214053","NEWT:80863","NEWT:105231","NEWT:52638","NEWT:57075","NEWT:4097","NEWT:8697","NEWT:8695","NEWT:884204","NEWT:9785","NEWT:6279","NEWT:1123869","NEWT:9544","NEWT:7370","NEWT:83906","NCBITaxon:780","NCBITaxon:1502","NEWT:6282","NEWT:1134506","NEWT:38783","NEWT:4006","NEWT:6426","NCBITaxon:5476","NEWT:6669","NEWT:9935","NEWT:89453","NEWT:287889","NEWT:51515","NEWT:588596","NEWT:51511","NEWT:8845","NEWT:92867","NEWT:92866","NEWT:5334","NEWT:51750","NEWT:202950","NEWT:382352","NEWT:41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