{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Txt":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/checksum.txt"],"Raw":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_01_05_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/211021_Lumos__LC1200__nanoFlex_NH21039_11_Ogly_targ.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_23_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_02_05_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210927_Lumos__LC1200__nanoFlex_NH21038_04_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210828_Lumos__LC1200__nanoFlex_NH21038_08_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_07_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210927_Lumos__LC1200__nanoFlex_NH21038_09_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_25_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210916_Lumos__LC1200__nanoFlex_NH21038_10_Ogly_targ.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_13_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_19_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_02_06_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_11_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_01_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_18_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_01_06_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_24_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_01_09_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_12_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_22_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_01_04_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_06_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_03_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_01_01_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_02_01_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_10_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_02_07_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_02_04_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_01_07_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/211021_Lumos__LC1200__nanoFlex_NH21039_01_Ogly_targ.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210927_Lumos__LC1200__nanoFlex_NH21038_02_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210927_Lumos__LC1200__nanoFlex_NH21038_05_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210927_Lumos__LC1200__nanoFlex_NH21038_08_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_22_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210927_Lumos__LC1200__nanoFlex_NH21038_01_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_02_08_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_01_02_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_01_08_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_04_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_10_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210927_Lumos__LC1200__nanoFlex_NH21038_07_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_20039_GlcNAc.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_16_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_26_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_01_03_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210927_Lumos__LC1200__nanoFlex_NH21038_06_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_09_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_15_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_02_03_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_25_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_02_09_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_27_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_21_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_26_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_17_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_23_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210927_Lumos__LC1200__nanoFlex_NH21038_03_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210916_Lumos__LC1200__nanoFlex_NH21038_09_Ogly_targ.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_05_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_11_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/211021_Lumos__LC1200__nanoFlex_NH21039_17_Ogly_targ.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210916_Lumos__LC1200__nanoFlex_NH21038_25_Ogly_targ.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_08_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210818_Lumos__LC1200__nanoFlex_NH21036_02_02_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_14_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_27_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/211021_Lumos__LC1200__nanoFlex_NH21039_25_Ogly_targ.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210929_Lumos__LC1200__nanoFlex_NH21039_02_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_24_Ogly.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/210928_Lumos__LC1200__nanoFlex_NH21038_20039_GalNAc.raw"],"Other":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029644/SEARCH.dat"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"labhead_mail":["hhw@sund.ku.dk"],"submitter":["Noortje de Haan"],"technology_type":["Mass Spectrometry","Top-down proteomics"],"software":[""],"submitter_keywords":["Hek293","Glycomics","Keratinocytes","O-glycan","Glycogenomics"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD029644"],"tissue":["Keratinocyte","Skin","Kidney"],"sample_protocol":["Protein immobilization, non-reductive beta-elimination of O-glycans, 2-aminobenzamide labeling, HILIC and PGC SPE, C18 nanoLC-MS/MS"],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["MS1 feature detection in the raw files was performed using the Minora Feature Detector node in Thermo Proteome Discoverer 2.2.0.388 (Thermo Fisher Scientific lnc.). The [M+H] values of the resulting features were imported into GlycoWorkbench 2.1 (build 146) [17] and matched to glycan compositions with 0 to 8 hexoses, 0 to 8 N-acetyl hexosamines, 0 to 3 fucoses, 0 to 4 N-acetyl neuraminic acids and a 2-AB label. An additional matching was performed to glycan compositions with 0 to 6 hexoses, 0 to 6 N-acetyl hexosamines, 0 to 2 fucoses, 0 to 2 N-acetyl neuraminic acids, 0 to 3 pentoses and a 2-AB label. The complete list of identified compositions was imported into Skyline 21.1.0.146 (ProteoWizard), using the Molecule Interface."],"omics_type":["Proteomics"],"labhead":["Hans Wandall"],"instrument_platform":[""],"labhead_affiliation":["University of Copenhagen, Copenhagen Center for Glycomics"],"submission_type":["PARTIAL"],"species":["Homo Sapiens (human)"],"publication":["35245040 de Haan N, Narimatsu Y, Koed Møller Aasted M, Larsen ISB, Marinova IN, Dabelsteen S, Vakhrushev SY, Wandall HH. In-Depth Profiling of <i>O</i>-Glycan Isomers in Human Cells Using C18 Nanoliquid Chromatography-Mass Spectrometry and Glycogenomics. Anal Chem. 2022 94(10):4343-4351 10.1021/acs.analchem.1c05068"],"submitter_mail":["ndehaan@sund.ku.dk"],"submitter_affiliation":["Copenhagen center for Glycomics, University of Copenhagen"],"submitter_country":["Denmark"],"pubmed_abstract":["<i>O</i>-Glycosylation is an omnipresent modification of the human proteome affecting many cellular functions, including protein cleavage, protein folding, and cellular signaling, interactions, and trafficking. The functions are governed by differentially regulated <i>O</i>-glycan types and terminal structures. It is therefore essential to develop analytical methods that facilitate the annotation of <i>O</i>-glycans in biological material. While various successful strategies for the in-depth profiling of released <i>O</i>-glycans have been reported, these methods are often limitedly accessible to the nonspecialist or challenged by the high abundance of <i>O</i>-glycan structural isomers. Here, we developed a high-throughput sample preparation approach for the nonreductive release and characterization of <i>O</i>-glycans from human cell material. Reducing-end labeling allowed efficient isomer separation and detection using C18 nanoliquid chromatography coupled to Orbitrap mass spectrometry. Using the method in combination with a library of genetically glycoengineered cells displaying defined <i>O</i>-glycan types and structures, we were able to annotate individual <i>O</i>-glycan structural isomers from a complex mixture. Applying the method in a model system of human keratinocytes, we found a wide variety of <i>O</i>-glycan structures, including <i>O</i>-fucose, <i>O</i>-glucose, <i>O</i>-GlcNAc, and <i>O</i>-GalNAc glycosylation, with the latter carrying both elongated core1 and core2 structures and varying numbers of fucoses and sialic acids. The method, including the now well-characterized standards, provides the opportunity to study glycomic changes in human tissue and disease models using rather mainstream analytical equipment."],"pubmed_title":["In-Depth Profiling of <i>O</i>-Glycan Isomers in Human Cells Using C18 Nanoliquid Chromatography-Mass Spectrometry and Glycogenomics."],"pubmed_authors":["de Haan Noortje N, Narimatsu Yoshiki Y, Koed Møller Aasted Mikkel M, Larsen Ida S B ISB, Marinova Irina N IN, Dabelsteen Sally S, Vakhrushev Sergey Y SY, Wandall Hans H HH"],"pubmed_title_synonyms":["Mass Spectrum Analysis, Mass Spectrum, polisacaridos, human being, Man (Taxonomy), Glycans, Analyses, Modern, Spectrometry, glycans, Glycane, Glykan, Spectrum Analyses, man, Spectrum Analysis, human, Cell, Glykane, polysaccharides, Human, Spectroscopy, polisacarido, MS, Homo sapiens, C18, Modern Man, D1, Glycan, B1, Mass, Mass., Analysis, Chromatographies, Man, Mass Spectrum Analyses, Polysaccharide, PTHB1, Mass Spectroscopy"],"description_synonyms":["apparatus, Specialist, Globular Protein Folding, Medical Devices, Glukose, A4, Monohydrate, Glykan, beta-tubulin folding, (DL)-Isomer, Specialism, Social Controls, dmTAF[[II]]230, polisacarido, Techniques, devices, diseases, Roles, Method, Dextrose, B1, Concepts, Specialists, diseases and disorders, Analysis, Formal Social Controls, Mass Spectrum Analysis, portion of tissue, human disease, appliances, Man (Taxonomy), TFIID TAF250, Glycans, Analyses, cel, Tissue, Glycane, procedures, DL-glucose, Folding, Social, chaperonin-mediated tubulin folding, Methodological Studies, Sialic, D1, Role Concepts, Glycan, Homo sapiens disease, Reference Standard, Standard Preparation, Standard Preparations, dTAF[[II]]230, Standardization, wide/broad, N Acetylneuraminic Acids, Devices, Modern, Liquid Chromatography, TAF200, N-Acetylneuraminic, TAFII-250, Procedure, TAF250/230, Spectrum Analysis, Glykane, Spectroscopy, chaperone activity, TAFII250, Role Concept, Globular Protein, co-chaperonin activity, Diseases, Role, Supplies and Equipment, tissue portion, alpha-tubulin folding, simple tissue, Protein Foldings, (alpha-D)-Isomer, END, D-Glucose, Standard, MS/MS, Instruments and Apparatus, Control, Spectrometry, Methodological, Controls, CG17603, TAF[[II]], Methodological Study, human, polysaccharides, disease, wide, co-chaperone activity, DMDA, Globular Protein Foldings, Taf250, D Glucose, SR3-5, multichaperone pathway, Acids, Equipment, Glucose Monohydrate, Regulation, Proteomes, TAF230, Regulations, other disease, d230, criteria, human being, Procedures, conformation, Glucose, nucleocapsid, Foldings, glycans, instruments, dTAFII250, Spectrum Analyses, broad, guidelines, EfW1, O-GLCNAC, TYPE, Human, DAGA4, method, glycosylation, Homo sapiens, Glycosylation, dmTAF1, Taf230, chaperonin ATPase activity, method used in an experiment, Studies, Mass, disease or disorder, non-chaperonin molecular chaperone ATPase activity, glycoprotein-specific chaperone activity, Carrying, Inventory., MAM, Globular, SCG3, Medical, Man, Technique, Polysaccharide, Mass Spectroscopy, TAF250, study, Taf200, Deoxygalactose, dTAF[[II]]250, cell, MS2, Taf1p, Fuc, labeling, man, Supplies, non-neoplastic, Study, dTAF250, 6-Deoxygalactose, Reference, Protein Folding, disorder, Preparation, TAF, relational structural quality, Anhydrous, HHT1, PTHB1, Medical Device, Apparatus and Instruments, Inventories, TAF[[II]]250, biological function, Edg, (beta-D)-Isomer, Protein Glycosylation, Formal Social Control, disorders, total expressed protein, l(3)84Ab, BG:DS00004.13, medical condition, Cell, LGMD2C, Concept, dTAF230, MS, Device, equipment, Social Control, C18, p230, Protein, TAF[[II]]250/230, core, TFIID, condition, techniques, protein complex assembly, Mass Spectrum Analyses, tandem MS, Taf[[II]]250, Mass Spectrum, polisacaridos, TAF[[II]]230, DMDA1, alpha Fucose, TAF[II]250, Keratinocyte, N-Acetylneuraminic Acids, Anhydrous Dextrose, plan specification, Preparations, DmelCG17603, alpha-Fucose, Modern Man, SCARMD2, Standards, regulation, ORW1, HRNT1, methodology, TAF1, Glc"],"sample_synonyms":["2-aminobenzamide, e(-), DELTA AND GAMMA, glycans, Gene, Glykan, protein, EK2-9, electron, betaTub1, protein-containing complex, CG1689, l(2)03350, protein polypeptide chains, polisacarido, B1t, polypeptide chain, B-spec, betaspec, B1, Gene Products, ortho-aminobenzamide, protein aggregate, E(Raf)2A, BcDNA:GM01870, Polysaccharide, poc, MS2., betatub(56D), kus, beta1tub, CG9277, Glycans, Elektron, l(2)k08102, beta[[1]] tubulin, beta1t, T, beta-particle, Glycane, E(Sev-CycE)2A, proteins, labeling, Sp4, 136/24, anon-EST:fe1B3, Splitends, 1t, e, CG16705, Hypokinesia, e-, beta1Tub, SPEC8, D1, Glycan, Experimental Hypokinesia, CG5870, SP4, DmelCG16705, PTHB1, beta-tubulin56D, Spec-beta, Dmbeta1, c-SP4, BETA 56D, protein complex, Proteins, Tubulin, En(yan[ACT])2-7, CT37173, i168, Glykane, Experimental, native protein, l(1)G0108, natural protein, spe, DmelCG1689, C18, Protein, Lz, fs(1)M69, betaSpec, rno, FLOWERING TIME CONTROL PROTEIN FCA ALPHA, b-Spec, tandem MS, beta-tub, DmelCG5870, polisacaridos, l(1)G0074, l(2)k07612, l(2)k06805, l(1)G0198, Beta <eudicots>, negatron, E(E2F)2A, beta[[1]]-tubulin, MS/MS, amx, CG18497, b spectrin, FCAALL.331, beta1, fs(1)A1569, beta56D, DmelCG9277, proPO-AE, EY2-7, beta, beta1-tub, beta(-), Protein Gene Products, DTB2, Tub, polysaccharides, Gene Proteins, beta-Tub, yip1, beta-spec, i6, DL4180C, DmelCG18497, Hypokinesias, BETA, beta-Tub56D, Spec, betaTub, Specbeta, Experimental Hypokinesias, beta1-Tubulin"],"pubmed_abstract_synonyms":["apparatus, Product, Globular Protein Folding, Medical Devices, Glukose, Monohydrate, Glykan, beta-tubulin folding, (DL)-Isomer, dmTAF[[II]]230, polisacarido, Techniques, devices, diseases, Method, Biological, Dextrose, B1, Extracts, diseases and disorders, Analysis, Biological Product, Mass Spectrum Analysis, 2-Acetamido-2-deoxygalactose, portion of tissue, Divorced, human disease, appliances, Man (Taxonomy), Biologic Drugs, TFIID TAF250, Glycans, Analyses, cel, Natural, Tissue, Glycane, Divorces, procedures, Biological Drugs, DL-glucose, Folding, chaperonin-mediated tubulin folding, Biological Medicine, Methodological Studies, signaling process, Sialic, D1, Glycan, Medicine, Homo sapiens disease, Medicines, Biologic Drug, Reference Standard, single organism signaling, RKS10, ELG, Standard Preparation, Standard Preparations, dTAF[[II]]230, Standardization, Biologic Products, N Acetylneuraminic Acids, Devices, Modern, TAF200, N-Acetylneuraminic, Procedure, TAFII-250, TAF250/230, Spectrum Analysis, Glykane, Spectroscopy, chaperone activity, TAFII250, Globular Protein, co-chaperonin activity, ELONGATED, Biopharmaceuticals, Diseases, Supplies and Equipment, tissue portion, Biologic Product, alpha-tubulin folding, simple tissue, Protein Foldings, (alpha-D)-Isomer, END, D-Glucose, Standard, Biological Medicines, Biological Drug, Instruments and Apparatus, Spectrometry, Biologics, BRI1-associated receptor kinase, Methodological, CG17603, Methodological Study, TAF[[II]], human, polysaccharides, organ system, Complex Extract, disease, co-chaperone activity, Globular Protein Foldings, Taf250, D Glucose, SR3-5, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, multichaperone pathway, Acids, Equipment, Glucose Monohydrate, Proteomes, TAF230, other disease, biological signaling, d230, criteria, human being, Procedures, Biologic Pharmaceuticals, Extract, Glucose, Foldings, glycans, instruments, dTAFII250, Spectrum Analyses, guidelines, EfW1, body system, Crude Extracts, Human, Crude, F17M5.190, method, Crude Extract, Complex Mixture, glycosylation, Homo sapiens, Glycosylation, dmTAF1, Taf230, chaperonin ATPase activity, method used in an experiment, Studies, Mass, disease or disorder, non-chaperonin molecular chaperone ATPase activity, glycoprotein-specific chaperone activity, system, Carrying, Inventory., Globular, Separated, SERK3, Medical, Man, Technique, Polysaccharide, Mass Spectroscopy, Biologicals, TAF250, Drugs, study, Taf200, Mixtures, Deoxygalactose, anatomical systems, dTAF[[II]]250, ATSERK3, cell, Complex, Natural Product, Taf1p, Fuc, labeling, man, Supplies, non-neoplastic, Study, dTAF250, 6-Deoxygalactose, Reference, Protein Folding, N-Acetylchondrosamine, disorder, Preparation, TAF, Chromatographies, Anhydrous, HHT1, Biologic, PTHB1, Pharmaceuticals, F17M5_190, Medical Device, Apparatus and Instruments, Products, Inventories, TAF[[II]]250, Edg, (beta-D)-Isomer, Protein Glycosylation, Biologic Medicines, Mixture, disorders, total expressed protein, l(3)84Ab, BG:DS00004.13, medical condition, Cell, dTAF230, MS, Device, equipment, C18, p230, Protein, Biopharmaceutical, GalNAc, TAF[[II]]250/230, TFIID, condition, techniques, connected anatomical system, protein complex assembly, Library, Mass Spectrum Analyses, Taf[[II]]250, Mass Spectrum, polisacaridos, Separation, RECEPTOR KINASES LIKE SERK 10, TAF[[II]]230, alpha Fucose, Separations, ATBAK1, TAF[II]250, Keratinocyte, N-Acetylneuraminic Acids, signalling, Anhydrous Dextrose, Drug, plan specification, Preparations, DmelCG17603, signalling process, alpha-Fucose, Modern Man, Standards, Natural Products, ORW1, methodology, Complex Extracts, TAF1, Glc"],"name_synonyms":["Human, human being, Man (Taxonomy), Homo sapiens, C18, D1, Modern Man, B1, Modern, Cell., man, Man, PTHB1, human"],"data_synonyms":["Hexose, oho-31, Bru, Stars, molecula., bs29g06.y1, Raw, Dalpha2, Feature, OHO31, alphaKap2, Dm NinaC, NINA C, CG4799, glycans, CG5125, Glykan, Pentose, pen, neutral molecular compounds, IMPalpha2, Kap-alpha2, importin alpha2/pendulin, DmelCG5125, Glykane, polisacarido, DmelCG4799, Characteristic, pen-2, Ms1, dorsal marginal zone, DPend, CT42491, Henson's node, l(2)k14401, Del(8)44H, NINAC, impalpha2, alpha2, Hexosamine, molecule, CG54125, Polysaccharide, anon-WO0140519.258, DRONINAC, Svc, polisacaridos, Col4a-1, l(2)144/1, molecules, NinaC, MS1, nodal stem, ninac, Glycans, MALE STERILITY 1 PROTEIN, nodus primitivus, Striated muscle activator of Rho-dependent signaling, imp alpha2, Kap alpha2, Dimp-alpha2, Glycane, Molekuel, T6G21.3, Neuraminic, oho31, Features, alpha2A-Kap, Ketopentoses, CG 5125, imp-alpha2, polysaccharides, Nina C, Ketopentose, Imp-alpha2, stem node, label, male sterility 1, Glycan, Kpna2, DMZ, Acids, Characteristics, 2.1, 2.2, STARS, Rch1, CT16120, Oho31"],"additional_accession":[]},"is_claimable":false,"name":"C18  nanoLC-MS and Glycogenomics to Facilitate O-Glycan profiling in Human Cells","description":"O-glycosylation is an abundant modification throughout the human proteome and plays a role in, amongst others, regulation of protein cleavage, protein folding, and modulation of cell-cell and -matrix interactions. O-glycosylation type and structure are important for biological function, demanding analytical methodologies that facilitate the annotation of O-glycans in biological material. While various successful strategies for the in-depth profiling of released O-glycans have been reported, these methods are often limitedly accessible to the non-specialist and/or challenged by the high abundance of O-glycan structural isomers. Here, we developed a high-throughput sample preparation approach for the non-reductive release of O-glycans from human cell material. Reducing-end labeling allowed efficient isomer separation and detection using C18 nano-liquid chromatography coupled to Orbitrap mass spectrometry (nanoLC-MS/MS). The implementation of glycogenetically engineered cell material proved a way into the complex structural annotation of O-glycan types and isomers. Using human keratinocytes as model system, we found a wide variety of O-glycan structures, including O-fucose, O-glucose, O-GlcNAc and O-GalNAc glycosylation, with the latter carrying both elongate Core 1 and Core 2 structures and varying numbers of fucoses and sialic acids. The method, including the now well characterized standards, provide the opportunity to study glycomic changes in human tissue and disease models using rather mainstream analytical equipment.","dates":{"publication":"2022-04-04","submission":"2021-11-10"},"accession":"PXD029644","cross_references":{"TAXONOMY":["NEWT:108018","NEWT:377960","NEWT:1131","NEWT:190486","NEWT:2042546","NEWT:148942","NEWT:295546","NEWT:258594","NEWT:1126","NEWT:1129","NEWT:89187","NEWT:309807","NEWT:89184","NEWT:309800","NEWT:1323429","NEWT:876138","NEWT:44271","NEWT:193516","NEWT:2315688","NEWT:592321","NEWT:1073882","NEWT:6819","NEWT:428386","NEWT:1117","NEWT:498257","NEWT:10036","NEWT:32264","NEWT:10034","NEWT:661410","NEWT:488003","NEWT:224326","NEWT:376619","NEWT:3765","NEWT:3767","NEWT:433727","NEWT:135622","NEWT:672127","NEWT:656061","NEWT:37965","NEWT:656064","NEWT:3760","NEWT:36630","NEWT:381198","NEWT:10029","NEWT:913645","NEWT:641809","NEWT:44250","NEWT:317447","NEWT:5530","NEWT:868565","NEWT:135674","NEWT:1562698","NEWT:12637","NEWT:2164133","NEWT:2767846","NEWT:1343074","NEWT:314344","NEWT:108061","NEWT:224308","NEWT:49928","NEWT:160621","NEWT:110368","NEWT:1916073","NEWT:1310161","NEWT:1230383","NEWT:77133","NEWT:1310165","NEWT:114727","NEWT:5516","NEWT:498211","NEWT:347256","NEWT:5518","NEWT:398007","NEWT:1527468","NEWT:498217","NEWT:498216","NEWT:11320","NEWT:1310173","NEWT:161934","NEWT:1148","NEWT:5508","NEWT:419481","NEWT:5507","NEWT:1140","NCBITaxon:2157","NEWT:2066563","NEWT:1143","NEWT:1462472","NEWT:1336795","NEWT:644042","NEWT:709964","NEWT:3712","NEWT:3711","NEWT:3714","NEWT:65121","NEWT:3715","NEWT:2065263","NEWT:1549675","NEWT:177437","NEWT:9014","NEWT:10891","NEWT:34869","NEWT:226126","NEWT:1616117","NEWT:34865","NEWT:550537","NEWT:284812","NEWT:3701","NEWT:3702","NEWT:1245466","NEWT:3704","NEWT:1246791","NEWT:34875","NEWT:9005","NEWT:34871","NEWT:9006","NEWT:33548","NEWT:1883426","NEWT:657321","NEWT:10886","NEWT:3708","NEWT:536231","NEWT:219813","NEWT:215451","NEWT:460519","NEWT:272843","NEWT:272844","NEWT:1348799","NEWT:55784","NEWT:485","NEWT:2499525","NEWT:142786","NEWT:2853422","NEWT:487","NEWT:1679718","NEWT:480","NEWT:67767","NEWT:46835","NEWT:216778","NEWT:376686","NEWT:237610","EFO:0001352","NEWT:219809","NEWT:219808","NEWT:119562","NEWT:1883446","NEWT:1233435","NEWT:118223","NEWT:141469","NEWT:272831","NEWT:987059","NEWT:491","NEWT:460523","NEWT:85694","NEWT:85692","NEWT:911045","NEWT:3750","NEWT:291146","NEWT:233271","NEWT:3745","NEWT:1715989","NCBITaxon:4751","NEWT:3747","NEWT:278161","NEWT:415978","NEWT:410289","NEWT:191701","NEWT:9054","NEWT:472","NEWT:29459","NEWT:28128","NEWT:470","NEWT:41122","NEWT:1328300","NEWT:748693","NCBITaxon:50557","NEWT:3735","NEWT:29491","NEWT:911022","NEWT:446","NEWT:153481","NEWT:445","NEWT:2003758","NEWT:63366","NEWT:63367","NEWT:29488","NEWT:215402","NEWT:3726","NEWT:30455","NEWT:28141","NEWT:9030","NEWT:9031","NEWT:3728","NEWT:108931","NEWT:219799","NEWT:219798","NEWT:548","NEWT:150475","NEWT:256737","NEWT:51350","NEWT:8694","NEWT:8692","NEWT:542","NEWT:394503","NEWT:13076","NEWT:219785","NEWT:451515","NEWT:550","NEWT:10804","NEWT:554","NEWT:451516","NEWT:552","NEWT:1325291","NEWT:42410","NEWT:1225786","NEWT:771870","NEWT:8665","NEWT:51338","NEWT:9995","NEWT:28112","NEWT:49012","NEWT:28110","NEWT:10832","NEWT:311339","NEWT:507601","NEWT:520","NEWT:64657","NEWT:186441","NEWT:2585209","NEWT:643680","NEWT:214092","NEWT:40697","NEWT:13095","NEWT:162425","NEWT:242124","NEWT:9986","NEWT:8654","NEWT:8658","NEWT:1268063","NEWT:8657","NEWT:9983","NEWT:8655","NEWT:80833","NEWT:479096","NEWT:28108","NEWT:28104","NEWT:1114957","NEWT:568703","NEWT:568708","NEWT:52641","NEWT:353152","NEWT:353153","NEWT:227377","NEWT:1774284","NEWT:27689","NEWT:207769","NEWT:40674","NEWT:51329","NCBITaxon:1763","NEWT:443906","NEWT:13037","NEWT:519","NEWT:941638","NEWT:7394","NEWT:6063","NEWT:404330","NEWT:1328388","NEWT:13064","NEWT:300641","NEWT:39655","NEWT:38323","NEWT:206411","NEWT:80869","NEWT:214053","NEWT:80863","NEWT:665890","NEWT:52639","NEWT:52638","NEWT:8697","NEWT:861109","NEWT:8695","NEWT:1196866","NEWT:6039","NEWT:1045775","NEWT:1123869","NCBITaxon:1335626","NEWT:7370","NEWT:280406","NEWT:83906","NEWT:99488","NEWT:1134506","NEWT:255470","NEWT:762903","NEWT:27697","NEWT:86185","NEWT:9935","NEWT:266940","NEWT:287889","NEWT:1028307","NEWT:52603","NEWT:92867","NEWT:92866","NEWT:1344414","NEWT:202950","NEWT:741158","NEWT:1293091","NEWT:202956","NEWT:315750","NEWT:632957","NEWT:9925","NCBITaxon:9606","NEWT:1436183","NEWT:1479235","NEWT:4232","NEWT:9922","NEWT:416348","NEWT:105812","NEWT:11298","NEWT:4233","NEWT:4236","NEWT:398031","NEWT:196627","NEWT:523840","NEWT:1076878","NEWT:200308","NEWT:200302","NEWT:9913","NEWT:9915","NEWT:105841","NEWT:1170227","NEWT:2666255","NEWT:999810","NEWT:28995","NEWT:1392998","NEWT:1150621","NEWT:1085791","NEWT:745531","NEWT:226900","NEWT:231490","NEWT:590651","NEWT:449239","NEWT:244704","NEWT:9909","NEWT:9902","NEWT:430615","NEWT:563041","NEWT:36599","NEWT:41949","NEWT:9900","NEWT:326423","NEWT:326424","NEWT:13013","NEWT:749906","NEWT:418985","NEWT:749907","NEWT:431947","NEWT:142809","NEWT:1095183","NEWT:302409","NEWT:9979","NEWT:130821","NEWT:2320211","NEWT:27606","NEWT:290610","NEWT:1519788","NEWT:59202","NEWT:9975","NEWT:8643","NEWT:1801631","NEWT:1159899","NEWT:502780","NEWT:860688","NEWT:35231","NEWT:1774208","NEWT:8644","NEWT:1125630","NEWT:202914","NEWT:222523","NEWT:27602","NEWT:502779","NEWT:297246","NEWT:126793","NEWT:483139","NEWT:101491","NEWT:8639","NEWT:8637","NEWT:1114970","NEWT:858373","NEWT:163735","NEWT:285272","NEWT:192222","NEWT:9963","NEWT:197911","NEWT:197912","NEWT:2785785","NEWT:82633","NEWT:1298722","NEWT:8626","NEWT:105884","NCBITaxon:9615","NEWT:8621","NEWT:8620","NEWT:74811","NEWT:8624","NEWT:8623","NEWT:5597","NEWT:218496","NEWT:218493","NEWT:8618","NEWT:265669","NEWT:70448","NEWT:9948","NEWT:1088878","NEWT:9940","NEWT:8612","NEWT:315749","NEWT:1247190","NEWT:2126719","NEWT:555479","NEWT:1538089","NEWT:6945","NEWT:2627626","NEWT:6943","NEWT:870726","NEWT:91374","NEWT:91373","NEWT:27804","NEWT:474186","NEWT:10136","NEWT:638301","NEWT:94400","NEWT:6941","NEWT:307972","NEWT:755969","NEWT:34","NEWT:200492","NEWT:1299114","NEWT:44386","NEWT:1590841","NEWT:1276473","NEWT:2282334","NEWT:1246","NEWT:1247","NEWT:70667","NEWT:869728","NEWT:6938","NEWT:1885322","NEWT:36774","NEWT:194963","NEWT:77298","NEWT:15819","NEWT:109461","NEWT:1085956","NEWT:1236","NEWT:1136157","NEWT:6928","NEWT:1239","NEWT:36745","NEWT:882735","NEWT:400727","NEWT:90062","NEWT:68335","NEWT:332952","NEWT:3885","NEWT:3888","NEWT:2653900","NEWT:32391","NEWT:6915","NEWT:101510","NEWT:3880","NEWT:216816","NEWT:1955775","NEWT:1220","NEWT:10140","NEWT:10141","NEWT:95767","NEWT:466585","NEWT:6918","NEWT:286706","NEWT:681196","NEWT:143361","NEWT:428268","NEWT:5658","NEWT:5656","NEWT:1292","NEWT:1454941","NEWT:31033","NEWT:1299","NEWT:1794699","NEWT:247149","NCBITaxon:183674","NEWT:167387","NEWT:6978","NEWT:1282","NEWT:1532924","NEWT:1283","NEWT:253836","NEWT:134471","NEWT:6973","NEWT:176279","NEWT:1288","NEWT:294381","NEWT:1390363","NEWT:406072","NEWT:526997","NEWT:316893","NEWT:751585","NEWT:113549","NEWT:159749","NEWT:1761792","NEWT:1270","NEWT:272129","NEWT:130081","NEWT:1348879","NEWT:360106","NEWT:360104","NEWT:10117","NEWT:10116","NEWT:1280","NEWT:2501646","NEWT:159736","NEWT:2951803","NEWT:2905945","NEWT:563924","NEWT:314262","NEWT:100226","NEWT:1263","NEWT:176299","NEWT:6953","NEWT:1654360","NEWT:743960","NEWT:314256","NEWT:1462594","NEWT:1034304","NEWT:105023","NEWT:866628","NEWT:535026","NEWT:749200","NEWT:1036967","NEWT:266264","NEWT:272942","NEWT:272943","NEWT:266265","NEWT:272944","NEWT:145953","NEWT:257309","NEWT:33658","NEWT:33657","NEWT:117008","NEWT:1131323","NEWT:1408157","NEWT:645463","NEWT:3821","NEWT:77216","NEWT:1111708","NEWT:93163","NEWT:257313","NEWT:3827","NEWT:400772","NEWT:257310","NEWT:930945","NEWT:591198","NEWT:445686","NEWT:106350","NEWT:1408186","NEWT:455045","NEWT:1860078","NEWT:54571","NEWT:412754","NEWT:2665964","NEWT:1423772","NEWT:53232","NEWT:1235987","NEWT:257329","NEWT:58933","NEWT:632138","NEWT:188229","NEWT:3818","NEWT:432359","NEWT:12721","NEWT:9103","NEWT:715877","NEWT:862238","NEWT:2665958","NEWT:6087","NEWT:145943","NEWT:595536","NEWT:89920","NEWT:3809","NEWT:207954","NEWT:3879","NEWT:1219","NEWT:411483","NEWT:3871","NEWT:3870","NEWT:411490","NEWT:169963","NEWT:584","NEWT:587","NEWT:588","NEWT:585","NEWT:29581","NCBITaxon:11084","NEWT:746128","NEWT:3864","NCBITaxon:1653394","NEWT:1205","NEWT:1207","NEWT:1108972","NEWT:1447716","NEWT:45609","NEWT:9172","NEWT:332966","NEWT:423477","NEWT:2592485","NEWT:1219067","NEWT:3854","NEWT:1080228","NEWT:1219065","NEWT:411469","NEWT:569","NEWT:411464","NEWT:411460","NEWT:322710","NEWT:1328426","NEWT:152275","NEWT:562","NEWT:411470","NEWT:2094720","NEWT:2545266","NEWT:1219071","NEWT:262724","NEWT:189513","NEWT:62141","NEWT:411479","NEWT:3848","NEWT:3847","NEWT:198466","NEWT:411476","NEWT:1080230","NEWT:1045010","NEWT:178876","NEWT:573","NEWT:571","NEWT:186497","NEWT:2599095","NEWT:29595","NEWT:42528","NEWT:1969471","NEWT:668","NEWT:150596","NEWT:30540","NEWT:439733","NEWT:76777","NEWT:30545","NEWT:76773","NEWT:29549","NEWT:1121114","NEWT:663","NEWT:1238993","NEWT:67825","NEWT:187878","NEWT:1504802","NEWT:633528","NEWT:1821314","NEWT:103351","NEWT:1249668","NEWT:300558","NEWT:426430","NEWT:223926","NEWT:37181","NEWT:365059","NEWT:7469","NEWT:1431450","NEWT:1160719","NEWT:1580596","NEWT:670","NEWT:426428","NEWT:99300","NEWT:115338","NEWT:115339","NEWT:63459","NCBITaxon:11071","NEWT:693977","NEWT:242231","NEWT:7454","NEWT:197221","NEWT:90988","NEWT:51453","NEWT:28231","NEWT:1121131","NEWT:30521","NEWT:7460","NEWT:504457","NEWT:1920175","NEWT:63430","NEWT:103372","NCBITaxon:11082","NEWT:151895","NEWT:226186","NEWT:659","NEWT:7445","NEWT:30531","NEWT:30532","NEWT:633513","NEWT:8782","NEWT:115357","NEWT:652","NEWT:63436","NEWT:28229","NEWT:1354724","NEWT:63433","NEWT:13164","NEWT:1601067","NEWT:943311","NEWT:1249615","NEWT:152335","NEWT:622","NEWT:623","NEWT:217219","NEWT:6192","NCBITaxon:1639","NEWT:63429","NEWT:3915","NEWT:405534","NEWT:1520022","NEWT:1214102","NEWT:651388","NEWT:633","NEWT:6182","NEWT:604162","NEWT:6185","NEWT:632","NEWT:6183","NEWT:1423800","NEWT:630","NCBITaxon:11019","NEWT:334747","NEWT:3907","NEWT:3906","NEWT:343691","NEWT:76717","NEWT:13180","NEWT:602","NEWT:39778","NEWT:170187","NEWT:414452","NEWT:259949","NEWT:493235","NEWT:692672","NEWT:568813","NEWT:267566","NEWT:267567","NEWT:58042","NEWT:643745","NEWT:1089448","NEWT:615","NEWT:1089449","NEWT:1089444","NEWT:6161","NEWT:206542","NEWT:186536","NEWT:3039","NEWT:2706014","NEWT:8727","NEWT:34062","NEWT:8726","NEWT:10092","NEWT:8725","NEWT:10090","NEWT:10091","NEWT:8729","NEWT:8720","NEWT:5692","NEWT:5693","NEWT:13125","NEWT:8724","NEWT:8723","NEWT:8722","NEWT:5697","NEWT:160791","NEWT:34073","NEWT:8715","NEWT:5689","NEWT:1547922","NEWT:10088","NEWT:1232801","NEWT:323259","NEWT:5690","NEWT:5691","NEWT:488732","NEWT:61674","NEWT:2072580","NEWT:669465","NEWT:1117957","NEWT:90100","NEWT:8709","NEWT:8707","NEWT:1306274","NEWT:5671","NEWT:13146","NEWT:8700","NEWT:3062780","NEWT:223998","NEWT:393962","NEWT:74968","NEWT:5661","NEWT:52714","NEWT:399266","NEWT:58002","NEWT:13136","NEWT:5660","NEWT:5665","NEWT:6997","NEWT:5666","NEWT:1746091","NEWT:5664","NEWT:146479","NEWT:905079","NEWT:124223","NEWT:27779","NEWT:6108","NEWT:1720309","NEWT:1286640","NEWT:3077","NEWT:6100","NEWT:3076","NEWT:112273","NEWT:8767","NEWT:1147787","NEWT:39717","NEWT:62977","NEWT:1391726","NEWT:8759","NEWT:27717","NEWT:10047","NEWT:8752","NEWT:34038","NEWT:8756","NEWT:74940","NEWT:10041","NEWT:1450511","NEWT:416276","NEWT:1857323","NEWT:59319","NEWT:890382","NEWT:10049","NEWT:1403337","NEWT:179392","NEWT:2823525","NEWT:1538186","NEWT:3055","NEWT:264462","NEWT:1450520","NEWT:741258","NEWT:82525","NEWT:82528","NEWT:235909","NEWT:149539","NEWT:1134458","NEWT:47311","NCBITaxon:813","NEWT:47314","NEWT:652616","NEWT:1335626","NEWT:104660","NEWT:652611","NEWT:1417983","NEWT:10068","NEWT:8732","NEWT:1417981","NEWT:36674","NEWT:2749149","NEWT:3555","NEWT:260201","NEWT:1005941","NEWT:35554","NEWT:935692","NEWT:10254","NEWT:2222","NEWT:59523","NEWT:544496","NEWT:31199","NEWT:59529","NEWT:1208660","NEWT:1392696","NEWT:1332145","NEWT:445974","NEWT:43179","NEWT:180454","NEWT:3544","NEWT:2214","NEWT:7901","NEWT:7904","NEWT:10245","NEWT:55153","NEWT:2944139","NEWT:10243","NEWT:59515","NEWT:10242","NEWT:273507","NEWT:351581","NEWT:43186","NEWT:70779","NEWT:135858","NEWT:64091","NEWT:2649997","NEWT:1091045","NEWT:1147161","NEWT:43150","NEWT:59509","NEWT:69781","NEWT:282458","NEWT:85831","NEWT:1333499","NEWT:3524","NEWT:866555","NEWT:1401257","NEWT:44491","NEWT:27933","NEWT:556484","NEWT:2283313","NEWT:7956","NEWT:7955","NEWT:81490","NEWT:7959","NEWT:645159","NEWT:35510","NEWT:2261","NEWT:31156","NEWT:7953","NEWT:126957","NEWT:45790","NEWT:284218","NEWT:1316932","NEWT:1316931","NEWT:45796","NEWT:6613","NEWT:113636","NEWT:931281","NEWT:658457","NEWT:35525","NEWT:122586","NEWT:2246","NEWT:2652724","NEWT:68415","NEWT:7936","NEWT:44447","NEWT:752555","NEWT:39097","NEWT:347495","NEWT:2242","NEWT:1324588","NEWT:391619","NEWT:633149","NEWT:1149786","NEWT:52065","NEWT:10239","NEWT:3567","NEWT:4897","NEWT:213863","NEWT:2239","NEWT:35500","NEWT:2041044","NEWT:3562","NEWT:4896","NEWT:1262161","NEWT:343904","NEWT:40091","NEWT:11557","NEWT:391624","NEWT:265948","NEWT:1773","NEWT:129117","NEWT:1772","NEWT:525338","NEWT:481672","NEWT:88925","NEWT:408169","NEWT:36809","NEWT:1762","NEWT:105135","NEWT:622488","NEWT:1764","NEWT:39087","NEWT:7067","NEWT:1760","NEWT:190650","NEWT:1986198","NEWT:7070","NEWT:408172","NEWT:408170","NEWT:283734","NEWT:7074","NEWT:1200793","NEWT:1513461","NEWT:1436733","NEWT:561896","NEWT:39053","NEWT:1247411","NEWT:430066","NEWT:333760","NEWT:1513458","NEWT:1233681","NEWT:2212966","NCBITaxon:40559","NEWT:57727","NEWT:506599","NEWT:451804","NEWT:84588","NEWT:1279007","NEWT:65349","NEWT:1733573","NEWT:300852","NEWT:1290255","NEWT:1744","NEWT:564933","NEWT:933084","NEWT:1121405","NEWT:1736604","NEWT:1080311","NEWT:366649","NEWT:7054","NCBITaxon:4932","NEWT:1748","NEWT:2764168","NEWT:943274","NEWT:3517","NEWT:118499","NEWT:256136","NEWT:290055","NEWT:476272","NEWT:3505","NEWT:561007","NEWT:147828","NEWT:55993","NEWT:203696","NEWT:223283","NEWT:505235","NEWT:1202531","NEWT:1202532","NEWT:29695","NEWT:41364","NEWT:319420","NEWT:1218097","NEWT:1080348","NEWT:2901879","NEWT:478009","NEWT:55986","NEWT:686","NEWT:408188","NEWT:571256","NEWT:688333","NCBITaxon:548681","NEWT:53326","NEWT:1781","NEWT:691","NEWT:28384","NEWT:2034244","NEWT:327042","NEWT:147802","NEWT:1872122","NEWT:7091","NEWT:85007","NEWT:41311","NEWT:67940","NEWT:1737068","NEWT:1813","NEWT:4087","NEWT:9778","NEWT:7111","NEWT:347515","NEWT:79923","NEWT:7116","NEWT:4090","NEWT:9780","NEWT:4092","NEWT:4093","NEWT:786","NEWT:4096","NEWT:941442","NEWT:536088","NEWT:311553","NEWT:1226408","NEWT:43984","NEWT:7108","NEWT:367110","NEWT:43989","NEWT:4076","NEWT:1561972","NEWT:293939","NEWT:9764","NEWT:4081","NEWT:29656","NEWT:98334","NEWT:4082","NEWT:1400821","NEWT:231663","NEWT:1400820","NEWT:4084","NEWT:63577","NEWT:1441379","NEWT:103481","NEWT:172044","NEWT:30640","NEWT:9753","NEWT:348824","NEWT:929793","NEWT:9760","NEWT:114155","NEWT:135062","NEWT:335543","NEWT:4072","NEWT:335541","NEWT:1337056","NEWT:4073","NEWT:148364","NEWT:102169","NEWT:4054","NEWT:777","NEWT:301301","NEWT:98351","NEWT:118503","NEWT:63558","NEWT:85057","NEWT:985076","NEWT:38581","NEWT:76856","NCBITaxon:565995","NEWT:7159","NEWT:267671","NEWT:72491","NEWT:7160","NEWT:98360","NEWT:8496","NEWT:7165","NEWT:1387333","NEWT:7162","NEWT:1859","NEWT:936053","NEWT:867900","NEWT:8479","NEWT:8485","NEWT:119856","NEWT:119857","NEWT:49240","NEWT:161398","NEWT:89426","NEWT:89427","NCBITaxon:3707","NEWT:449447","NEWT:58172","NEWT:1249997","NEWT:936046","NEWT:38568","NEWT:38569","NEWT:727","NEWT:9796","NEWT:725","NEWT:7137","NEWT:726","NEWT:469008","NEWT:123901","NEWT:1836","NEWT:573039","NEWT:49212","NCBITaxon:777","NEWT:105231","NEWT:396513","NEWT:206672","NEWT:9788","NEWT:4097","NEWT:436113","NEWT:732","NEWT:71139","NEWT:9785","NEWT:241073","NEWT:1195774","NEWT:9793","NCBITaxon:780","NEWT:4006","NCBITaxon:5475","NCBITaxon:5476","NEWT:6668","NEWT:6669","NEWT:89453","NEWT:1114301","NEWT:51515","NEWT:13249","NEWT:51511","NEWT:7998","NEWT:419610","NEWT:5334","NEWT:82829","NCBITaxon:714","NEWT:553482","NEWT:6657","NEWT:89462","NEWT:2758385","NEWT:6655","NEWT:714","NEWT:5322","NEWT:421932","NEWT:312017","NEWT:576131","NEWT:553470","NEWT:100392","NEWT:5315","NEWT:265872","NEWT:90461","NEWT:59463","NEWT:337330","NEWT:72433","NEWT:2285","NEWT:2287","NEWT:6645","NEWT:251229","NEWT:104759","NEWT:338654","NEWT:88149","NEWT:46147","NEWT:5306","NEWT:174633","NEWT:52830","NEWT:1416333","NEWT:7962","NEWT:640319","NEWT:145262","NEWT:145263","NEWT:1250153","NEWT:1333524","NEWT:9739","NEWT:111069","NEWT:696747","NEWT:1379791","NEWT:8400","NEWT:2750454","NEWT:264561","NEWT:544580","NEWT:46124","NEWT:1333550","NEWT:46122","NEWT:48784","NEWT:675814","NEWT:72407","NEWT:180498","NEWT:352914","NCBITaxon:7227","NEWT:157295","NEWT:9720","NEWT:9721","NEWT:2008793","NEWT:60423","NEWT:9715","NEWT:1009408","NEWT:1298968","NEWT:5353","NEWT:9711","NEWT:6689","NEWT:5358","NEWT:60419","NEWT:2902295","NEWT:252598","NEWT:61742","NEWT:46105","NEWT:104782","NEWT:10181","NEWT:9704","NEWT:13216","NEWT:128735","NEWT:5341","NEWT:10184","NEWT:10185","NEWT:521001","NEWT:1813887","NEWT:648242","NEWT:267608","NEWT:334249","NEWT:184922","NEWT:6703","NEWT:262981","NEWT:2","NEWT:35670","NEWT:6706","NEWT:35677","NEWT:10376","NEWT:3674","NEWT:935568","NEWT:1365186","NEWT:1813019","NEWT:997828","NEWT:134676","NEWT:310915","NEWT:1348623","NEWT:35688","NEWT:1000364","NEWT:11696","NEWT:1736548","NEWT:10360","NEWT:3663","NEWT:47664","NEWT:317548","NEWT:317547","NEWT:2323","NEWT:3654","NEWT:3656","NEWT:3659","NEWT:59620","NEWT:1047005","NEWT:31298","NEWT:1204162","NEWT:1532734","NEWT:82688","NCBITaxon:4892","NEWT:1712536","NCBITaxon:4895","NCBITaxon:4896","NEWT:3649","NEWT:910263","NEWT:101755","NEWT:3641","NEWT:1245528","NEWT:73908","NEWT:264722","NEWT:478749","NEWT:704171","NEWT:137732","NEWT:1659840","NEWT:12993","NEWT:1053","NEWT:1264690","NEWT:31276","NEWT:318829","NEWT:443239","NEWT:34305","NEWT:10335","NEWT:44586","NEWT:1736582","NEWT:1250057","NEWT:431241","NEWT:443226","NEWT:144036","NEWT:1169286","NEWT:34316","NEWT:34317","NEWT:76164","NEWT:144030","NEWT:288705","NEWT:1250050","NEWT:392815","NEWT:374990","NEWT:36941","NEWT:154293","NEWT:3694","NEWT:210007","NEWT:96405","NEWT:10359","NEWT:10358","NEWT:1735272","NEWT:317513","NEWT:6715","NEWT:580240","NEWT:11676","NEWT:55210","NEWT:1220207","NEWT:44577","NEWT:1295830","NEWT:1895","NEWT:4922","NEWT:414717","NEWT:414718","NEWT:64152","NEWT:4924","NEWT:1524259","NEWT:33892","NEWT:11620","NEWT:516507","NEWT:990346","NEWT:152496","NEWT:1640346","NEWT:4929","NEWT:44544","NEWT:529818","NEWT:4911","NEWT:152480","NEWT:156845","NEWT:129249","NEWT:1888","NEWT:131568","NEWT:723287","NEWT:1144889","NEWT:943129","NEWT:131567","NEWT:1221594","NEWT:1524260","NEWT:76126","NEWT:271848","NEWT:308678","NEWT:637616","NEWT:35608","NEWT:1402601","NEWT:106590","NEWT:45882","NEWT:4918","NCBITaxon:95619","NEWT:106592","NEWT:596645","NEWT:218843","NEWT:1187947","NEWT:4903","NEWT:10312","NCBITaxon:1773","NEWT:1432499","NEWT:872292","NEWT:431275","NEWT:10319","NEWT:1477405","NEWT:36909","NEWT:4909","NEWT:759272","NEWT:1182263","NEWT:943146","NEWT:646532","NEWT:537457","NEWT:479249","NEWT:7167","NEWT:217509","NEWT:435623","NEWT:33889","NEWT:10306","NEWT:12968","NEWT:12967","NEWT:12966","NEWT:3057131","NEWT:3633","NEWT:3635","NEWT:39152","NEWT:1306859","NEWT:2050920","NEWT:12916","NEWT:1400870","NEWT:225117","NEWT:4952","NEWT:396334","NEWT:1266464","NEWT:367830","NEWT:4959","NEWT:3627","NEWT:2862456","NEWT:1093790","NEWT:95145","NEWT:371094","NEWT:2497473","NEWT:375451","NEWT:54788","NEWT:52132","NEWT:74381","NEWT:2010198","NEWT:167539","NEWT:83167","NEWT:316275","NEWT:395019","NEWT:316273","NEWT:1174673","NEWT:304292","NEWT:1313166","NEWT:2582415","NEWT:613619","NEWT:11602","NEWT:349161","NEWT:4932","NEWT:40137","NEWT:3603","NEWT:4934","NEWT:12922","NEWT:316277","NEWT:163159","NEWT:227748","NEWT:2813599","NCBITaxon:11292","NEWT:190802","NEWT:7234","NEWT:9899","NEWT:1216979","NEWT:9895","NEWT:7237","NEWT:2608663","NEWT:9897","NEWT:578822","NEWT:28450","NEWT:502057","NEWT:7241","NEWT:7240","NEWT:8570","NEWT:127569","NEWT:29780","NEWT:1241935","NEWT:186786","NEWT:1797482","NEWT:41447","NEWT:7227","NEWT:193079","NEWT:7226","NEWT:287375","NEWT:336722","NEWT:675060","NEWT:288676","NEWT:1330547","NEWT:1985738","NEWT:7215","NEWT:196139","NEWT:9874","NEWT:651182","NCBITaxon:59201","NEWT:7220","NEWT:882","NEWT:434271","NEWT:1915","NEWT:66712","NEWT:1051067","NEWT:42752","NEWT:53408","NEWT:49390","NEWT:9870","NEWT:1261557","NEWT:4182","NEWT:1125722","NEWT:435590","NEWT:1902","NEWT:208526","NEWT:1908","NEWT:217459","NEWT:126283","NEWT:132961","NEWT:216129","NEWT:12072","NEWT:129314","NEWT:299391","NEWT:198732","NEWT:51671","NEWT:80966","NEWT:863","NEWT:254472","NEWT:126279","NEWT:1228997","NEWT:1441467","NEWT:62335","NEWT:366581","NEWT:757417","NEWT:1051075","NEWT:693745","NEWT:42736","NEWT:1607839","NEWT:990316","NEWT:194366","NEWT:1121308","NEWT:1218707","NEWT:198726","NEWT:99586","NEWT:2060328","NEWT:62322","NEWT:48038","NEWT:311400","NEWT:49369","NEWT:392021","NEWT:51655","NEWT:201089","NEWT:8595","NEWT:7263","NEWT:841","NEWT:280754","NEWT:225058","NEWT:186763","NEWT:185431","NEWT:928303","NEWT:29760","NEWT:703612","NCBITaxon:11266","NEWT:1295743","NCBITaxon:439488","NEWT:1505907","NEWT:1249885","NEWT:853","NEWT:7244","NEWT:1415167","NEWT:36036","NCBITaxon:662","NEWT:851","NEWT:71251","NEWT:1168853","NEWT:378548","NEWT:63632","NEWT:181088","NEWT:63631","NEWT:669202","NEWT:9818","NEWT:1193806","NEWT:1250006","NEWT:4120","NEWT:823","NEWT:820","NEWT:821","NEWT:1097","NEWT:37334","NEWT:2040765","NEWT:49317","NEWT:1899318","NEWT:696811","NEWT:9808","NEWT:839","NEWT:9803","NEWT:1231719","NEWT:37347","NEWT:943925","NEWT:4111","NEWT:9801","NEWT:831","NEWT:1085","NEWT:37349","NEWT:4113","NEWT:837","NEWT:640459","NEWT:589554","NEWT:72568","NEWT:51641","NEWT:12022","NEWT:334147","NEWT:277944","NEWT:201999","NEWT:1206777","NEWT:1906951","NEWT:52945","NEWT:4100","NEWT:1076","NEWT:4102","NEWT:1028688","NEWT:4101","NEWT:6763","NEWT:803","NEWT:29722","NEWT:221570","NEWT:37319","NEWT:144042","NEWT:13373","NEWT:88036","NEWT:6756","NEWT:817","NEWT:818","NEWT:128810","NEWT:1061","NEWT:72543","NEWT:72542","NEWT:29719","NEWT:1063","NEWT:6754","NEWT:813","NEWT:1365218","NEWT:1042986","NEWT:1480154","NEWT:9858","NEWT:9859","NEWT:1705394","NEWT:2907833","NEWT:38626","NEWT:10298","NEWT:10299","NEWT:9852","NEWT:9860","NEWT:660025","NEWT:660027","NEWT:1238391","NEWT:90371","NEWT:55194","NEWT:2907829","NEWT:4153","NEWT:1320353","NEWT:9844","NEWT:72520","NEWT:59552","NEWT:39966","NEWT:58228","NEWT:915099","NEWT:59557","NEWT:687914","NEWT:348780","NEWT:1199150","NEWT:862962","NEWT:859313","NEWT:59540","NEWT:52904","NEWT:9837","NEWT:9838","NEWT:9839","NEWT:546414","NEWT:9833","NEWT:9835","NEWT:4146","NEWT:5478","NEWT:35572","NEWT:5476","NEWT:8502","NEWT:5477","NEWT:322159","NEWT:746707","NEWT:5482","NEWT:1206781","NEWT:5480","NEWT:222863","NEWT:61861","NEWT:859321","NEWT:35581","NEWT:9825","NEWT:39946","NEWT:9823","NEWT:39947","NEWT:382641","NEWT:39948","NEWT:859314","NEWT:60520","NEWT:330879","NEWT:35795","NEWT:3311","NEWT:47770","NEWT:259447","NEWT:1334565","NEWT:625351","NCBITaxon:1280","NEWT:112503","NEWT:59753","NEWT:281395","NEWT:247480","NEWT:1211601","NEWT:1367493","NEWT:46456","NEWT:553199","NEWT:80379","NEWT:46429","NEWT:475174","NEWT:1598","NEWT:1599","NEWT:12215","NEWT:1590","NEWT:12211","NEWT:33114","NEWT:1596","NEWT:2736291","NEWT:638632","NEWT:294671","NEWT:1748418","NCBITaxon:79857","NEWT:1096976","NEWT:1589","NEWT:4615","NEWT:35786","NEWT:1580","NEWT:399784","NEWT:96731","NEWT:1582","NEWT:1584","NEWT:2086595","NEWT:1585","NEWT:383379","NCBITaxon:12721","NEWT:1392488","NEWT:38815","NEWT:4688","NEWT:3357","NEWT:7719","NEWT:29073","NEWT:11786","NEWT:3352","NEWT:79329","NEWT:79327","NEWT:30069","NEWT:59729","NEWT:295105","NEWT:29065","NEWT:60711","NEWT:2020713","NEWT:3347","NEWT:1220585","NEWT:3349","NEWT:2502409","NEWT:212790","NEWT:55350","NEWT:33103","NEWT:706473","NEWT:145481","NEWT:29058","NCBITaxon:79824","NEWT:468822","NEWT:410658","NEWT:47715","NEWT:44688","NEWT:44689","NEWT:370038","NEWT:345076","NEWT:305899","NEWT:246196","NEWT:246197","NEWT:145458","NEWT:200795","NEWT:342808","NEWT:2579791","NEWT:614666","NEWT:44685","NEWT:3329","NEWT:260499","NEWT:410661","NEWT:329854","NEWT:2649329","NEWT:99802","NEWT:1211620","NEWT:1287689","NEWT:1094343","NEWT:150808","NEWT:242605","NEWT:1182590","NEWT:156974","NCBITaxon:37162","NEWT:199225","NEWT:717966","NEWT:10418","NEWT:118698","NEWT:118696","NEWT:52283","NEWT:8175","NEWT:43330","NEWT:1603293","NEWT:29022","NEWT:44664","NEWT:43346","NEWT:244366","NEWT:96795","NEWT:176597","NEWT:118694","NEWT:2850","NEWT:118691","NEWT:57975","NEWT:52299","NEWT:10407","NEWT:8165","NEWT:96794","NEWT:10401","NEWT:332648","NEWT:44670","NEWT:926571","NEWT:376219","NEWT:1510","NEWT:1955249","NEWT:1515","NEWT:572307","NEWT:1432138","NEWT:2714896","NEWT:1424507","NEWT:1194599","NEWT:1608994","NEWT:1432137","NEWT:33995","NEWT:1303443","NEWT:329032","NEWT:56636","NEWT:55307","NEWT:1122981","NEWT:1122984","NEWT:1538553","NEWT:45972","NEWT:2830","NEWT:109757","NEWT:582580","NEWT:1185650","NEWT:1502","NEWT:128017","NEWT:2836","NEWT:95486","NEWT:31345","NEWT:11757","NEWT:109760","NEWT:29031","NEWT:317047","NEWT:1509","NEWT:45954","NEWT:1578","NEWT:235443","NEWT:1579","NEWT:1757312","NEWT:109786","NEWT:1922344","NEWT:108458","NEWT:5936","NEWT:321967","NEWT:320637","NEWT:1078283","NEWT:983964","NEWT:11706","NEWT:32644","NEWT:527796","NEWT:1392498","NEWT:279010","NEWT:499175","NEWT:109779","NEWT:1116234","NEWT:235455","NEWT:1255228","NEWT:373153","NEWT:1640278","NEWT:1092586","NEWT:940825","NCBITaxon:485","NEWT:529507","NEWT:1071661","NEWT:109784","NEWT:5911","NEWT:39251","NEWT:101841","NEWT:2014887","NEWT:717989","NEWT:2880","NEWT:33952","NEWT:1255232","NEWT:11723","NEWT:153009","NEWT:1004879","NEWT:1504303","NEWT:261756","NEWT:166314","NEWT:166313","NEWT:1871049","NEWT:1547","NEWT:1290454","NEWT:906914","NEWT:32630","NEWT:81231006","NEWT:156981","NEWT:27292","NEWT:1050722","NEWT:1293497","NEWT:1055524","NEWT:2902","NEWT:267872","NEWT:908627","NEWT:381666","NEWT:172269","NEWT:9534","NEWT:2126348","NEWT:5180","NEWT:299467","NEWT:631220","NEWT:9541","NEWT:33936","NEWT:2903","NEWT:185579","NEWT:872227","NEWT:2587410","NEWT:207340","NEWT:1006581","NEWT:9527","NEWT:33940","NEWT:2126337","NEWT:253237","NEWT:33941","NEWT:36185","NEWT:106774","NEWT:1054211","NEWT:106773","NEWT:575412","NEWT:49495","NCBITaxon:573","NEWT:6493","NEWT:114398","NEWT:6494","NEWT:6491","NCBITaxon:6157","NEWT:104105","NEWT:216257","NEWT:138336","NEWT:5147","NEWT:187303","NEWT:407821","NEWT:691692","NCBITaxon:2","NEWT:1613","NEWT:8247","NEWT:8245","NEWT:986","NEWT:2233643","NEWT:118781","NEWT:28532","NEWT:298176","NEWT:640131","NEWT:1194669","NEWT:106788","NEWT:8236","NEWT:74426","NEWT:204274","NEWT:996","NEWT:30871","NEWT:496866","NEWT:2510939","NEWT:8240","NEWT:257632","NEWT:105487","NEWT:1548728","NEWT:667127","NEWT:9557","NEWT:377586","NEWT:964","NEWT:9554","NEWT:475932","NEWT:1384040","NEWT:256318","NCBITaxon:6191","NEWT:229533","NEWT:204232","NEWT:28550","NEWT:576784","NEWT:1592886","NEWT:90675","NEWT:57075","NEWT:1666905","NEWT:976","NEWT:75736","NEWT:884204","NEWT:9544","NEWT:9545","NEWT:979","NEWT:30850","NEWT:1204414","NEWT:38783","NEWT:6426","NEWT:33090","NEWT:50429","NEWT:305959","NEWT:13489","NEWT:29829","NCBITaxon:3055","NEWT:51750","NEWT:621823","NEWT:441772","NEWT:484021","NEWT:1867051","NEWT:557600","NEWT:48106","NEWT:295027","NEWT:148545","NCBITaxon:11320","NEWT:313590","NEWT:135248","NEWT:90690","NEWT:300273","NEWT:7742","NEWT:99020","NEWT:1879010","NEWT:63737","NEWT:441768","NEWT:941279","NEWT:870435","NEWT:58370","NEWT:1191523","NEWT:881122","NEWT:57047","NCBITaxon:5693","NEWT:2144189","NEWT:380394","NEWT:521674","NEWT:1266738","NEWT:7725","NEWT:883796","NEWT:448385","NEWT:33083","NEWT:72664","NEWT:249161","NEWT:452467","NEWT:198822","NEWT:283035","NEWT:36111","NEWT:1678078","NEWT:150847","NEWT:2023717","NEWT:28502","NEWT:29835","NEWT:69014","NEWT:101028","NEWT:13443","NEWT:33059","NEWT:546991","NEWT:5141","NEWT:46360","NCBITaxon:3044782","NEWT:220341","NEWT:486644","NEWT:160235","NEWT:103690","NEWT:1928434","NEWT:2095","NEWT:2096","NEWT:152600","NEWT:2097","NEWT:2099","NEWT:7787","NEWT:546981","NEWT:1617910","NEWT:637379","NEWT:212717","NEWT:12107","NEWT:486636","NEWT:411901","NEWT:186802","NEWT:186801","NEWT:411903","NEWT:219334","NEWT:58334","NEWT:58331","NEWT:6446","NEWT:33038","NEWT:5116","NEWT:6447","NEWT:33035","NEWT:194439","NCBITaxon:12637","NEWT:38727","NEWT:406560","NEWT:420850","NEWT:47678","NEWT:447094","NEWT:103695","NEWT:569628","NEWT:300251","NEWT:33043","NEWT:13451","NEWT:118702","NEWT:118700","NEWT:118705","NEWT:411916","NEWT:72859","NEWT:3435","NEWT:2104","NEWT:157546","NEWT:1414448","NEWT:54195","NEWT:321803","NEWT:38942","NEWT:4762","NEWT:2102","NEWT:1037890","NEWT:38949","NEWT:60876","NCBITaxon:6854","NCBITaxon:39054","NEWT:1736309","NEWT:582645","NEWT:4751","NEWT:1415784","NEWT:860918","NEWT:12336","NEWT:272568","NEWT:272560","NEWT:272561","NEWT:272563","NEWT:11009","NEWT:913998","NEWT:649408","NEWT:272557","NEWT:272559","NEWT:153151","NEWT:2681598","NEWT:515849","NEWT:1000589","NEWT:42192","NEWT:85963","NEWT:85962","NEWT:42197","NEWT:1223501","NEWT:180763","NEWT:1434011","NEWT:1085537","NEWT:329726","NEWT:6500","NEWT:316407","NEWT:1561005","NEWT:592010","NEWT:84645","NEWT:1123294","NEWT:209285","NEWT:3469","NEWT:211586","NEWT:747078","NEWT:30195","NEWT:4792","NEWT:1000561","NEWT:2133","NEWT:2134","NEWT:197","NEWT:35889","NEWT:351660","NEWT:29176","NEWT:201319","NEWT:1073569","NEWT:150949","NEWT:4787","NEWT:46503","NEWT:180788","NEWT:613463","NEWT:388919","NEWT:4784","NEWT:4785","NEWT:401614","NEWT:83333","NEWT:83334","NEWT:292178","NEWT:242718","NEWT:83331","NEWT:83332","NEWT:161","NEWT:555088","NEWT:242715","NEWT:1867057","NEWT:573729","NEWT:1094460","NEWT:56780","NEWT:2110","NEWT:1450134","NEWT:54126","NEWT:75058","NEWT:13616","NEWT:13613","NEWT:35869","NEWT:179","NEWT:262316","NEWT:296543","NEWT:173","NEWT:316435","NEWT:42157","NEWT:165179","NEWT:1882682","NEWT:537209","NEWT:369822","NEWT:56777","NEWT:203124","NEWT:34508","NEWT:1095705","NEWT:230741","NEWT:8296","NEWT:167748","NEWT:1163394","NEWT:2972","NEWT:749696","NEWT:294747","NEWT:986075","NEWT:320549","NEWT:990119","NEWT:143786","NEWT:11855","NEWT:95340","NEWT:998086","NEWT:228410","NEWT:493760","NEWT:157","NEWT:128161","NEWT:332521","NEWT:1978007","NEWT:3068400","NEWT:945556","NEWT:29170","NEWT:1411316","NEWT:269796","NEWT:1349767","NEWT:9598","NEWT:1867944","NEWT:52389","NEWT:51056","NEWT:35815","NEWT:552857","NEWT:1639","NEWT:68730","NCBITaxon:37296","NEWT:8255","NEWT:139","NEWT:29159","NEWT:1155748","NEWT:9593","NEWT:8260","NEWT:9595","NEWT:246200","NEWT:94032","NEWT:9592","NEWT:240906","NEWT:592022","NEWT:29158","NEWT:44745","NEWT:479436","NEWT:44744","NEWT:1328881","NEWT:373042","NEWT:76336","NEWT:479437","NEWT:884019","NEWT:1694","NEWT:51031","NEWT:212035","NEWT:65672","NEWT:214693","NEWT:82076","NEWT:239935","NEWT:1680","NEWT:280148","NEWT:2770979","NEWT:119219","NEWT:112","NEWT:107243","NEWT:1277351","NEWT:1594786","NEWT:40366","NEWT:214687","NEWT:1679","NEWT:333849","NEWT:214684","NEWT:1758543","NEWT:525283","NEWT:202697","NEWT:10515","NCBITaxon:2697049","NEWT:44720","NEWT:294750","NEWT:1663","NEWT:214675","NEWT:61294","NEWT:65651","NEWT:64320","NEWT:2806551","NEWT:327159","NEWT:437603","NEWT:1660","NEWT:327160","NEWT:1131757","NEWT:142479","NEWT:572477","NEWT:241368","NEWT:471472","NEWT:9656","NEWT:9657","NEWT:437552","NEWT:527000","NEWT:9662","NEWT:1081927","NEWT:2483431","NEWT:929439","NEWT:9646","NEWT:5286","NEWT:9643","NEWT:9644","NEWT:53655","NCBITaxon:446","NEWT:2652157","NEWT:2652158","NEWT:40353","NEWT:1890364","NEWT:40321","NEWT:40324","NEWT:588858","NEWT:9638","NEWT:9639","NEWT:528352","NEWT:158053","NEWT:405948","NEWT:528354","NEWT:1177189","NEWT:528353","NEWT:308056","NEWT:1279085","NEWT:392227","NEWT:763924","NEWT:1004024","NEWT:291613","NEWT:862751","NEWT:669874","NEWT:470150","NEWT:9627","NEWT:470152","NEWT:1009714","NEWT:527031","NEWT:73239","NEWT:38018","NEWT:381518","NEWT:5270","NEWT:2562237","NEWT:1055687","NEWT:129554","NEWT:40302","NEWT:343232","NEWT:1736","NEWT:382835","NEWT:343237","NEWT:382834","NEWT:2664179","NEWT:7038","NEWT:767468","NEWT:767465","NEWT:1581419","NEWT:244447","NEWT:9689","NEWT:74546","NEWT:74547","NEWT:8355","NEWT:9685","NEWT:1218948","NEWT:7029","NEWT:9688","NEWT:9694","NEWT:1046723","NEWT:186990","NEWT:8364","NEWT:654898","NEWT:27315","NEWT:61235","NEWT:393595","NEWT:1331678","NEWT:1457551","NEWT:15368","NEWT:586231","NEWT:9678","NEWT:1491466","NEWT:340188","NEWT:1755437","NEWT:1042399","NEWT:9681","NEWT:1717","NEWT:1718","NEWT:1719","NEWT:2697049","NEWT:7004","NEWT:9669","NCBITaxon:6073","NEWT:1329349","NEWT:607699","NEWT:27337","NEWT:575584","NEWT:257771","NEWT:185687","NEWT:6549","NEWT:91891","NEWT:2186","NEWT:283166","NEWT:439334","NEWT:6544","NEWT:218192","NCBITaxon:10114","NEWT:1634342","NEWT:1685931","NEWT:242619","NEWT:557722","NEWT:1353008","NEWT:5207","NEWT:351746","NEWT:402042","NEWT:373995","NEWT:3040162","NEWT:544404","NEWT:287535","NEWT:2173","NEWT:13599","NEWT:2175","NEWT:1047176","NEWT:1263550","NEWT:6535","NEWT:667282","NEWT:38881","NEWT:6526","NEWT:1064539","NEWT:1064535","NEWT:3494","NEWT:6523","NEWT:1692259","NEWT:1486917","NEWT:1229621","NEWT:1843183","NEWT:157592","NEWT:3482","NEWT:36237","NEWT:51866","NEWT:88271","NEWT:3483","NEWT:1179777","NEWT:94951","NEWT:2157","NEWT:70125","NEWT:1843192","NEWT:206189","NEWT:2160","NEWT:295139","NEWT:181214","NEWT:426066","NEWT:9612","NEWT:589363","NEWT:38865","NEWT:9615","NEWT:713604","NEWT:589366","NEWT:2250255","NEWT:13563","NEWT:33178","NEWT:114452","NEWT:2753605","NEWT:2753603","NEWT:2562269","NEWT:1440772","NEWT:45157","NEWT:301447","NEWT:9605","NEWT:9606","NEWT:641501","NEWT:9601","NEWT:9604","NEWT:13551","NEWT:1437113","NEWT:9600","NEWT:439375","NEWT:174934","NEWT:115778","NEWT:82996","NEWT:92323","NEWT:92320","NEWT:1659668","NEWT:227086","NEWT:195883","NEWT:1042311","NEWT:1123352","NEWT:29920","NEWT:524867","NEWT:127785","NEWT:708248","NEWT:29916","NEWT:94993","NEWT:6550","NEWT:33169","NEWT:763037","NEWT:1250232","NEWT:1052588","NEWT:1377","NEWT:71647","NEWT:11103","NCBITaxon:12939","NEWT:1322343","NEWT:32042","NEWT:11102","NEWT:32046","NEWT:1175663","NEWT:32049","NEWT:762376","NEWT:45351","NEWT:1851651","NEWT:1142745","NEWT:5722","NEWT:32051","NEWT:295319","NEWT:323098","NEWT:1263034","NEWT:44031","NEWT:1209525","NEWT:428146","NEWT:1358","NEWT:54291","NEWT:498019","NEWT:1351","NEWT:1438992","NEWT:1352","NEWT:32025","NEWT:1645733","NEWT:263737","NEWT:69304","NEWT:5702","NEWT:95648","NEWT:478434","NEWT:1349","NEWT:67593","NEWT:100787","NEWT:1263054","NEWT:94328","NEWT:71605","NEWT:94323","NEWT:3112","NEWT:8801","NEWT:4442","NEWT:192875","NEWT:1214915","NEWT:1214916","NEWT:10699","NEWT:169683","NEWT:511145","NEWT:213401","NEWT:4432","NEWT:5762","NEWT:5763","NEWT:118099","NEWT:32019","NEWT:420247","NEWT:1424337","NEWT:295358","NEWT:1298593","NEWT:420245","NEWT:5757","NCBITaxon:4563","NEWT:5755","NEWT:5759","NEWT:1736231","NEWT:1392","NEWT:35974","NEWT:1396","NEWT:286","NEWT:287","NEWT:2789892","NEWT:1499987","NEWT:68895","NEWT:114517","NEWT:68888","NEWT:5744","NEWT:72901","NEWT:1905158","NEWT:210352","NEWT:55571","NEWT:1300146","NEWT:5740","NEWT:1472037","NEWT:5741","NEWT:1314781","NEWT:296","NEWT:13735","NEWT:1390","NEWT:562716","NEWT:294","NEWT:82380","NEWT:63186","NEWT:42249","NEWT:935293","NEWT:375146","NEWT:11983","NEWT:471876","NEWT:9258","NEWT:1090134","NEWT:264","NEWT:263","NEWT:11988","NEWT:568076","NEWT:400667","NEWT:387127","NEWT:379504","NEWT:1408274","NEWT:47946","NEWT:109974","NEWT:413996","NEWT:243277","NEWT:243274","NEWT:243275","NEWT:586722","NEWT:243273","NEWT:274","NEWT:59919","NEWT:186156","NEWT:155864","NEWT:3932","NEWT:42229","NEWT:85431","NEWT:29292","NEWT:9238","NEWT:450950","NEWT:13708","NEWT:34607","NEWT:35938","NEWT:1349409","NEWT:1451139","NEWT:10677","NEWT:81077","NEWT:85433","NEWT:67525","NEWT:3920","NEWT:508771","NEWT:253","NEWT:494514","NCBITaxon:2759","NEWT:93061","NEWT:93062","NEWT:1284813","NEWT:34613","NEWT:10665","NEWT:108673","NEWT:1488406","NEWT:162145","NEWT:644223","NEWT:1263082","NEWT:64471","NEWT:272623","NEWT:1288389","NEWT:272624","NEWT:886559","NEWT:55521","NEWT:412694","NEWT:526225","NEWT:1130798","NEWT:385388","NEWT:423349","NEWT:55529","NEWT:1089176","NEWT:272621","NEWT:195051","NEWT:3988","NEWT:86794","NEWT:649908","NEWT:1001135","NEWT:3981","NEWT:195058","NEWT:3983","NEWT:35925","NEWT:352472","NEWT:235","NEWT:55518","NEWT:97441","NEWT:1154756","NEWT:632245","NEWT:234","NCBITaxon:12066","NEWT:360094","NEWT:1408223","NEWT:1408224","NEWT:1311","NEWT:1313","NEWT:1314","NEWT:39491","NEWT:212142","NCBITaxon:5811","NEWT:338188","NEWT:3972","NEWT:1499105","NEWT:192812","NEWT:552536","NEWT:204516","NEWT:40483","NEWT:1119529","NEWT:155894","NEWT:1303","NEWT:318586","NEWT:518634","NEWT:1304","NCBITaxon:5820","NEWT:1307","NEWT:272634","NEWT:198107","NEWT:1979401","NEWT:9267","NEWT:155892","NEWT:167879","NEWT:1308","NEWT:1309","NEWT:1034015","NEWT:760568","NEWT:141262","NEWT:306","NEWT:505693","NEWT:303","NEWT:97477","NEWT:113287","NEWT:503034","NEWT:435258","NEWT:6269","NEWT:11901","NEWT:208895","NEWT:184485","NEWT:1111678","NEWT:273075","NEWT:40479","NEWT:403677","NEWT:142615","NEWT:316","NEWT:317","NEWT:885319","NEWT:6253","NEWT:497515","NEWT:885318","NEWT:39488","NEWT:298386","NEWT:2013786","NEWT:273068","NEWT:1316582","NEWT:227166","NEWT:2884427","NEWT:2732007","NEWT:7574","NEWT:1715256","NEWT:6248","NEWT:1507179","NEWT:29204","NEWT:160490","NEWT:634955","NEWT:371601","NEWT:6239","NEWT:208893","NEWT:1263854","NEWT:2059687","NEWT:160488","NEWT:61376","NEWT:207559","NEWT:107401","NEWT:39432","NEWT:425011","NEWT:2702","NEWT:1215323","NEWT:8008","NEWT:1257118","NEWT:8010","NEWT:1315282","NEWT:27448","NEWT:2708","NEWT:38111","NEWT:39442","NEWT:405416","NEWT:235612","NEWT:243232","NEWT:243230","NEWT:243231","NEWT:1080772","NEWT:1037908","NEWT:72000","NEWT:2893330","NEWT:391295","NEWT:1170828","NEWT:1249515","NEWT:2049433","NEWT:1170827","NEWT:93934","NEWT:9315","NEWT:6289","NEWT:6287","NEWT:391290","NEWT:9321","NEWT:596153","NCBITaxon:10298","NEWT:1652998","NEWT:60013","NEWT:104385","NEWT:74649","NEWT:1037924","NEWT:643867","NEWT:9305","NEWT:6279","NEWT:69293","NCBITaxon:1502","NEWT:6282","NEWT:27457","NEWT:1872635","NEWT:113208","NEWT:11060","NEWT:1215360","NEWT:1566338","NEWT:51997","NEWT:588596","NEWT:382359","NEWT:8845","NEWT:8843","NEWT:382352","NEWT:413071","NCBITaxon:10239","NEWT:332097","NEWT:1076620","NEWT:1356638","NEWT:11051","NEWT:452680","NEWT:8839","NEWT:4472","NEWT:171101","NEWT:568996","NCBITaxon:10245","NCBITaxon:10243","NCBITaxon:10244","NCBITaxon:10242","NEWT:284593","NEWT:655819","NEWT:11082","NEWT:284590","NEWT:588581","NEWT:69266","NEWT:981087","NEWT:180066","NEWT:57270","NEWT:36352","NCBITaxon:6312","NEWT:11072","NEWT:35024","NEWT:135461","NEWT:670580","NEWT:99287","NEWT:1817808","NEWT:1121088","NEWT:296587","NEWT:509173","NEWT:694569","NEWT:51953","NEWT:57267","NEWT:7552","NEWT:3197","NEWT:13686","NEWT:1402135","NEWT:36329","NEWT:62615","NEWT:1555636","NEWT:147493","NEWT:646099","NEWT:349741","NEWT:75917","NEWT:409859","NEWT:1263871","NEWT:75913","NEWT:509169","NEWT:6210","NEWT:137221","NEWT:565050","NEWT:409860","NEWT:219595","NEWT:92683","NEWT:2218628","NEWT:1034809","NEWT:452659","NEWT:1193501","NEWT:51930","NEWT:1193502","NEWT:6204","NEWT:8867","NEWT:194675","NEWT:12374","NEWT:36307","NEWT:46580","NEWT:298315","NEWT:392547","NEWT:90037","NEWT:1049056","NEWT:698936","NEWT:1009874","NEWT:4498","NEWT:8855","NEWT:11034","NEWT:3168","NEWT:78168","NEWT:192011","NEWT:224911","NEWT:1090946","NCBITaxon:11552","NEWT:1498","NEWT:5855","NEWT:4528","NEWT:136841","NEWT:1492","NEWT:1493","NEWT:1496","NEWT:5851","NEWT:4521","NEWT:660469","NEWT:45474","NCBITaxon:5759","NEWT:1219690","NEWT:48500","NEWT:4513","NEWT:1488","NEWT:5843","NEWT:110205","NEWT:110202","NEWT:58781","NEWT:1117645","NCBITaxon:10407","NEWT:1758316","NEWT:341663","NEWT:1403536","NEWT:1427524","NEWT:260799","NEWT:557436","NEWT:106198","NEWT:237561","NEWT:5833","NEWT:70415","NEWT:1268634","NEWT:499229","NEWT:671143","NEWT:56115","NEWT:127906","NEWT:5823","NEWT:5821","NEWT:44139","NEWT:570417","NEWT:675512","NEWT:315393","NEWT:28903","NEWT:1211705","NEWT:11234","NEWT:13894","NEWT:28909","NEWT:79698","NEWT:28901","NEWT:4568","NEWT:45438","NEWT:4567","NCBITaxon:5796","NEWT:398580","NEWT:4565","NEWT:515619","NEWT:302004","NEWT:170721","NEWT:77020","NEWT:1908323","NEWT:648794","NEWT:83558","NEWT:626528","NEWT:4556","NEWT:139927","NEWT:4558","NEWT:5888","NEWT:515622","NEWT:2705415","NEWT:4550","NEWT:932011","NEWT:4555","NEWT:3217","NEWT:4547","NEWT:3218","NEWT:556182","NEWT:1113279","NEWT:1432059","NEWT:156471","NEWT:5874","NEWT:156476","NEWT:4543","NCBITaxon:11709","NEWT:1857017","NEWT:1796613","NEWT:1358430","NEWT:1381693","NEWT:109298","NEWT:2448046","NEWT:707678","NEWT:294128","NEWT:1917265","NEWT:34774","NEWT:718308","NEWT:4530","NEWT:5861","NEWT:2714340","NEWT:4532","NEWT:75299","NEWT:2558360","NEWT:215358","NEWT:2748","NEWT:153348","NEWT:198252","NEWT:8049","NEWT:99931","NEWT:1608611","NEWT:1978224","NEWT:9378","NEWT:527640","NEWT:1267361","NEWT:100816","NEWT:192952","NEWT:656367","NEWT:387","NCBITaxon:1313","NEWT:29382","NEWT:29385","NEWT:527639","NEWT:400540","NEWT:382","NEWT:1404","NEWT:1077286","NCBITaxon:171","NEWT:10760","NEWT:8040","NEWT:227321","NEWT:492670","NEWT:281677","NEWT:260710","NEWT:1408","NEWT:2156843","NEWT:1162296","NEWT:41045","NEWT:46704","NEWT:42345","NEWT:482428","NEWT:85310","NEWT:323848","NEWT:190304","NEWT:56992","NEWT:1383439","NEWT:8030","NEWT:1211776","NEWT:246432","NEWT:8031","NEWT:246437","NEWT:1279365","NEWT:2711","NEWT:374","NCBITaxon:131567","NEWT:375","NEWT:34739","NEWT:8022","NEWT:42353","NEWT:593117","NEWT:319761","NEWT:5811","NEWT:2787","NEWT:86662","NEWT:43658","NEWT:180923","NEWT:86661","NEWT:1451","NEWT:198215","NEWT:200298","NEWT:343","NEWT:178606","NEWT:84005","NEWT:34708","NEWT:8090","NEWT:5801","NEWT:398514","NEWT:233150","NEWT:43665","NEWT:5802","NEWT:233155","NEWT:267386","NEWT:178616","NEWT:358","NEWT:357","NCBITaxon:186538","NEWT:258441","NCBITaxon:186536","NEWT:28005","NEWT:457428","NEWT:84023","NEWT:589904","NEWT:559292","NEWT:307796","NEWT:2762","NEWT:329","NEWT:1417298","NEWT:457425","NEWT:28035","NEWT:28034","NEWT:119488","NEWT:690307","NEWT:323","NEWT:322","NEWT:157728","NCBITaxon:138","NEWT:29365","NEWT:28038","NEWT:1422","NEWT:84030","NEWT:1423","NEWT:319795","NEWT:1205903","NEWT:2756","NEWT:2759","NEWT:38293","NEWT:1428","NEWT:339","NEWT:457431","NEWT:2751","NEWT:330","NEWT:139077","NEWT:391904","NEWT:1306413","NEWT:518766","NEWT:2664373","NEWT:1213463","NEWT:9417","NEWT:5059","NEWT:55601","NEWT:5060","NEWT:31631","NEWT:30301","NEWT:509924","NEWT:115104","NEWT:5061","NEWT:5062","NEWT:1398558","NEWT:1408434","NEWT:220668","NEWT:9407","NEWT:1214786","NEWT:679895","NEWT:9402","NEWT:415540","NEWT:416870","NEWT:334542","NEWT:313625","NEWT:51240","NEWT:27592","NEWT:5052","NEWT:227289","NEWT:31646","NEWT:444079","NEWT:27598","NEWT:408","NEWT:5037","NEWT:2172103","NEWT:1876254","NEWT:388399","NEWT:747679","NEWT:60162","NEWT:84074","NEWT:746360","NEWT:89764","NEWT:543734","NEWT:1045610","NEWT:5025","NEWT:256648","NEWT:334564","NEWT:1571871","NEWT:1169740","NEWT:1245012","NEWT:2886930","NEWT:1479564","NEWT:171250","NEWT:347834","NEWT:528135","NEWT:8128","NEWT:8127","NEWT:1637254","NCBITaxon:38727","NEWT:176946","NEWT:1954183","NEWT:74790","NEWT:2829","NEWT:1428006","NEWT:216599","NEWT:216595","NEWT:40559","NEWT:51208","NEWT:216597","NEWT:392499","NEWT:1852274","NEWT:1283300","NEWT:1204551","NEWT:228577","NEWT:1107493","NEWT:40520","NEWT:40521","NEWT:41856","NEWT:436486","NEWT:1933298","NEWT:1043002","NEWT:260707","NEWT:121845","NEWT:5085","NEWT:436495","NEWT:329199","NEWT:1431647","NEWT:447135","NEWT:216591","NEWT:216594","NEWT:260704","NEWT:260705","NEWT:546805","NEWT:9430","NEWT:203275","NEWT:208963","NEWT:208964","NEWT:310571","NEWT:48498","NEWT:6306","NEWT:7634","NCBITaxon:10357","NCBITaxon:10358","NCBITaxon:10359","NEWT:1203258","NEWT:1097670","NEWT:92519","NEWT:8954","NEWT:35168","NEWT:81866","NEWT:1262469","NEWT:555778","NEWT:28847","NEWT:48479","NEWT:61435","NEWT:627025","NEWT:1097677","NEWT:375939","NEWT:1797891","NEWT:712938","NEWT:3258","NEWT:188908","NEWT:953739","NEWT:112018","NEWT:101364","NEWT:1159556","NEWT:44080","NEWT:273133","NEWT:415593","NEWT:61428","NEWT:419947","NEWT:2847306","NEWT:184545","NEWT:468911","NEWT:554476","NEWT:266834","NEWT:672404","NEWT:7604","NEWT:266835","NEWT:135588","NEWT:7609","NEWT:4571","NEWT:452589","NEWT:509068","NEWT:2794344","NEWT:8932","NEWT:4577","NEWT:337677","NEWT:2086510","NEWT:1911079","NEWT:69373","NEWT:188912","NEWT:427577","NEWT:264203","NEWT:570509","NEWT:264201","NEWT:1080800","NEWT:1761012","NEWT:512623","NEWT:1274414","NEWT:477816","NEWT:1087439","NEWT:61408","NEWT:48420","NEWT:1355477","NEWT:202809","NEWT:11137","NEWT:278839","NEWT:35128","NEWT:7668","NEWT:6337","NEWT:8996","NEWT:1143193","NEWT:681376","NEWT:1122233","NEWT:1263753","NEWT:265554","NEWT:56031","NEWT:152952","NEWT:11167","NEWT:7650","NEWT:37751","NEWT:6326","NEWT:7656","NEWT:1882945","NEWT:299767","NEWT:28835","NEWT:1274432","NEWT:2053667","NEWT:1274426","NEWT:105751","NEWT:1274423","NEWT:393765","NEWT:96939","NEWT:104421","NEWT:95602","NEWT:6313","NEWT:1262449","NEWT:56029","NCBITaxon:11676","NEWT:1749399","NEWT:1143179","NEWT:69332","NEWT:578454","NEWT:1274420","NEWT:709323","NEWT:578458","NEWT:49743","NEWT:181486"],"pubmed":["35245040"],"ORCID":["0000-0001-7026-6750"]}}