Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_37_AspNsemi.msfViewftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/2942-2_ChT-G_5-30_acNeuAc_PrecQuant_QEHF_190815_48.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/2942-2_G_AspN-ns_5-30_acNeuAc_PrecQuant_QEHF_190402_12.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_12_ChTmc4_NtoD.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_36_AspNsemi.msfViewftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_16_GluCsemi_NtoD.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_28-_2_.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_08_T_NtoD.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_33.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/2942-2_T-G_5-30_acNeuAc_PrecQuant_QEHF_190402_08.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_29.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_32.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_16_AspNsemi_NtoD.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_36_GluCsemi.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/2942-2_G_GluC-ns_5-30_acNeuAc_PrecQuant_QEHF_190402_12.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_37_GluCsemi.msfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/O-glycan_6_most_common.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/N-glycan_database_2.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/N-glycan_database_1.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/File_list.pdfftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_28.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_32.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_29.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_37.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_180815_48.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_16.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_33.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190402_12.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_08.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190402_08.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_12.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_36.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_33_PrecQuant_INT.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_16_PNGaseF_NtoD_PrecQuant_GluCsemi_INT.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/2942-2_G_GluC-ns_5-30_acNeuAc_PrecQuant_QEHF_190402_12.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_08_PNGaseF_NtoD_PrecQuant_T_INT.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/2942-2_ChT-G_5-30_acNeuAc_PrecQuant_QEHF_190815_48.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/2942-2_T-G_5-30_acNeuAc_PrecQuant_QEHF_190402_08.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/2942-2_G_AspN-ns_5-30_acNeuAc_PrecQuant_QEHF_190402_12.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_28_PrecQuant_INT.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_37_PrecQuant_GluCuns_INT.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_12_PNGaseF_NtoD_PrecQuant_ChT_INT.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_36_PrecQuant_GluCuns_INT.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190528_16_PNGaseF_NtoD_PrecQuant_AspNsemi_INT.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_29_PrecQuant_INT.pdResultftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD031334/QEHF_190204_32_PrecQuant_INT.pdResultprimaryOK200ekaterina.mirgorodskaya@gu.seProteomics Core FacilityMass SpectrometryShotgun proteomicsGlycoproteomicsRatHuman papillintegrinsLiverLc-ms/msOrbitraphttps://www.ebi.ac.uk/pride/archive/projects/PXD031334LiverPurified a5b1 integrin integrin (75 µg) at a concentration of 0.5 mg/ml in 10 mM Tris, 150 mM NaCl, 10 mM EDTA, 0.06 % Triton X-100, pH 7.4, was reduced for 30 min at 56 °C with 100 mM DTT and processed using the modified filter-aided sample preparation (FASP) method {Wiśniewski, 2009 #43}. In short, reduced sample was divided into three equal parts, with each part transferred to an individual 30 kDa MWCO Pall Nanosep centrifugation filter (Pall Corporation). Filters were washed twice with digestion buffer (1 % SDOC in 50 mM TEAB). An additional wash with digestion buffer was performed after the alkylation of the samples with 18 mM IAM for 30 min at room temperature (in the dark). Four different enzymes were selected for proteolysis to provide an optimal access to glycansthe glycosylation sites. Individual filters were incubated with 75 µl of digestion buffer containing either one of the selected enzymes or enzyme combinations, i.e., (i) 0.25 µg trypsin overnight followed by extra incubation with 0.25 µg trypsin for 3 h, both at 37 °C, (ii) 0.5 µg chymotrypsin over night at room temperature, and (iii) 0.4 µg GluC overnight followed by incubation with 0.5 µg AspN for 3 h, both at 37 °C. Proteolytic peptides were collected by centrifugation and filters were additionally washed with 35 µl of 50 mM TEAB to ensure that all material was eluted from the filters. Neuraminidase treated a5b1 integrin (115 µg) at 0.5 mg/ml in 10 mM Tris, 150 mM NaCl, 10 mM EDTA, 0.06 % Triton X-100, pH 7.4, was digested using the same procedures as described above, adjusted for higher amount of starting material, i.e., (i) 0.3 µg trypsin overnight followed by extra incubation with 0.3 µg trypsin for 3 h, both at 37C, (ii) 0.5 µg chymotrypsin over night at room temperature, and (iii) 0.5 µg GluC overnight followed by incubation with 0.7 µg AspN for 3 h, both at 37 °C. All proteolytic preparations were first purified using HiPPR™ spin columns, followed by acidification with 10 % TFA to remove remaining SDOC. All peptide preparations were desalted using Pierce peptide desalting spin columns (Thermo Scientific) prior to LC-MS/MS analysis. The detergent free preparations were dried down and reconstituted to final concentration of 0.7 µg/µl in 2 % ACN, 0.1 % FA for LC-MS analysis. To evaluate the presence of O-glycosylation, one third of the purified peptide material from each proteolytic preparation of the neuraminidase treated a5b1 integrin was further treated overnight at 37 oC with 20 units of recombinant PNGaseF in 20 µl of 50 mM TEAB (Promega.com), desalted using peptide desalting spin columns, dried down and reconstituted in 2 % acetonitrile (ACN) in 0.1 % formic acid (FA) for LC-MS analysis. Digested samples were analyzed on an QExactive HF mass spectrometer interfaced with Easy-nLC1200 liquid chromatography system (Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Fischer Scientific) and separated on an in-house packed analytical column (75 μm x 300 mm, particle size 3 μm, Reprosil-Pur C18, Dr. Maisch) using a gradient from 7 % to 50 % B over 75 min, followed by an increase to 100 % B for 5 min at a flow of 300 nL/min, where Solvent A was 0.2 % FA and solvent B was 80 % ACN in 0.2 % FA. The instrument operated in data-dependent mode where the precursor ion mass spectra were acquired at a resolution of 120,000 for two separate m/z ranges, 375-1,500 and 600-2,000. The 10 most intense ions with charge states 2 to 5 were selected for fragmentation using higher-energy collision dissociation (HCD) at the normalized collision energy settings of 28. The isolation window was set to 3 m/z and dynamic exclusion to 10 ppm for 20 s. MS2 spectra were recorded at a resolution of 30,000 with maximum injection time set to 110 ms.PrideThe acquired data were analyzed using Proteome Discoverer version 2.2 and 2.4 (Thermo Fisher Scientific). Database searches were performed with either Byonic (Protein Metrics) or Mascot as search engines. Initially the data were searched with Mascot against the Uniprot Rodentia database to evaluate the purity of the protein preparations. All glycoproteomic searches were then performed with Byonics against a smaller custom database (12,484 entries) containing a5 and b1 integrins sequences (Uniprot A0A0G2K1E2 and A0A0G2JSK5, respectively). Precursor mass tolerance was set to 10 ppm and fragment mass tolerance to 30 ppm. Tryptic peptides with up to two missed cleavages and chymotryptic peptides with up to five missed cleavages were accepted. Variable modification of methionine oxidation and fixed cysteine alkylation were allowed. Semi-specific cleavage settings were used for AspN/GluC preparations as searches with simultaneous C- and N-terminal cleavages are not allowed by Proteome Discoverer. Different N-glycosylation databases were used depending on the type of preparation, i.e., with or without sialic acid containing structures. The databases were optimized, based on the detected oxonium ions and the available knowledge of the N-glycan biosynthesis, to allow for all expected N-glycans but with consideration of the search space size. Target decoy was used for PSM validation. Prior to the final assignment, the identified glycosylated peptides were manually evaluated based on the observed fragmentation pattern, the number of glycolforms per site, the number of PSM per glycoform, and the retention time windows for the different glycoforms (at the same site). The extracted ion chromatogram (EIC) peak intensities were used to determine the glycoform abundances, expressed as percent of total signal for all modified and non-modified peptides sharing the same amino acid sequence.ProteomicsEkaterina MirgorodskayaPARTIALProteomics Core Facility at University of Gothenburg Box 413, SE 405 30 Gothenburg, SwedenRattus Norvegicus (rat)Homo Sapiens (human)10.1111/BOC.202200017gupcf@outlook.comSAMBIO Core Facilities, Sahgrenska Academy, University of GothenburgSwedenRat, alphaPS2, norvegicus, Protein Glycosylation, conformation, Laboratory, PS2, Integrin, glycans, Norway Rat, Glykan, Rattus, Norway Rats, Glykane, aPS2, CT27194, polisacarido, rat, Rattus sp. strain Wistar, in, Mus norvegicus, Rats, Rattus norwegicus, glycosylation, Glycosylation, Mammals, Laboratory Rats, Protein, Norway rat, alpha2Int, Rattus rattiscus, region, If, Polysaccharide, relational structural quality., brown rat, polisacaridos, Rattus norvegicus, alpha[[PS2]], Glycans, Glycane, CG9623, rats, integrin, Laboratory Rat, inf, polysaccharides, DmelCG9623, alphaPS2C, Glycan, PS 2, PS2alpha, alpha[[PS2(ms8)]], site, Norway, PSalpha2, relational structural quality, Rattus norvegicus8, Gunn ratsQa1, sodium salt, ammonium formate, 13C-labeled, Size, MeCN, cadmium salt, ion, Laundry Pods, TFA, Liquid Chromatography Mass Spectrometry, Particle, dNSF, Octoxinols, Triton X305, Syndet, Ultra Performance Liquid Chromatography-Mass Spectrometry, magnesium formate, Apaf-1, zinc salt, heavy chain disease, Solvent, Long Term, GCase, l(2)k09905, 5730420M11Rik, Polypeptides, HPLC-MS, cobalt(II) formate dihydrate, Method, CH3-C#N, B1, 1, 3, alpha2Int, WMS, Octoxynol-9, DmelCG8428, Ultra Performance Liquid Chromatography Mass Spectrometry, SET, Spin, C79325, i2pp2a., hac-1, DmelCG13176, ORF19, HardwareType, AI047805, DmelCG4299, salt, set, SPIN, DmelCG9623, column, BTKAP1, T18c(37), ACN, sample, Acn, D1, GTFII-I, Octylphenoxy Polyethoxyethanol, nickel salt, SGS, 6030441I21Rik, LC-MS2, Detergent Pod, Degradations, cobalt (+2) salt, anon-53Fa, e(Pc), Liquid Chromatography, Longterm Effect, l(2)SH0173, Procedure, BAP135, Triton X100, not genetically inherited, CG3428, ACMICD, Avazyme, common salt, sodium (4:1:1) salt, Dm1, magnesium salt, Sizes, Laundry Detergent Pod, Cleansing Agents, 1110049F14Rik, BAP-135, Detergent Pods, CG6829, H15r/nmr2, dark/hac-1/dapaf-1, DmelCG7776, HLA-DR-associated protein II, instrument, DI-2, High Pressure Liquid Chromatography Mass Spectrometry, I-2Dm, Protein Digestions, formate, Spectrometry, cromium (+3), Methodological, GtfII-I, beta Trypsin, GC, I-2PP1, l(2)W5, TAF-IBETA, liquid chromatography-tandem mass spectroscopy, DmelLcp3, BG:DS02740.11, Charges and Fees, ammonium (4:1) salt, NSF-1, TAF-Ibeta, 3-tetramethylbutyl)phenyl)-omega-hydroxy-, methyl cyanide, lead (+2) salt, Polyethoxyethanol, Particle Sizes, GH05739, nickel formate dihydrate, Oligosaccharide Sialidase, lead formate, Effects, Biocatalysts, aluminum salt, Pierce, NSF1, precursor, thomson, 2-ethanediyl), 17alpha)-isomer, body system, CG7826, mass-to-charge ratio, Agent, in, GH14582, Triton X 305, H2-Qa1, LC-MSMS, CG7835, CG42273, system, Qa-1(b), Technique, If, DmelCG2043, Slrr1c, Synthetic, UPLC-MS, anatomical systems, FAM39E, lcp3, T23d, Acinus, T23b, thallium (+1) salt, Longterm, beta-Trypsin, LCP-3, rubidium salt, 2pp2a, Spectrometries, Long-Term, methanoic acid, ions, alpha-(4-(1, CG10574, Laundry Detergent, CD156, acinusL, Study, Injectable, Enzyme, DmelCG6634, CP3, 2PP2A, alphaPS2C, Triton X45, acinusS, Kochsalz, PS 2, dSET, dSet, peptidos, PTHB1, com, WBSCR6, dark/dapaf-1/hac-1, LcpIII, 1-(14)C-labeled, Protein Glycosylation, dApaf-1, Octoxinol, High Pressure Liquid Chromatography-Mass Spectrometry, potassium formate, copper (+2) salt, acetonitrile, buffer, Digestions, mKIAA0670, bnch, CD142, l(2)k02511, polypeptide, Triton X 100, Wash2, APAF1, Wash1, H15r, Temperatures, Natriumchlorid, Proteolyses, Hac-1, I-2PP2A, C18, cupric formate, chemical analysis, Dm I-2, Long Term Effects, 5alpha, MFS1, dNSF-1, NaCl, LCP3, aluminum formate, AU020952, lithium formate, DMLCP3, underdeveloped, ensemble, l(2)28-28-12, Aspn, Exo alpha Sialidase, CG6634, Alpha-Chymotrypsin Choay, Dark/Dapaf-1/HAC1, extra, ethanenitrile, CD156a, Longterm Effects, ME-IV, plan specification, DmelCG5861, Fees, alpha[[PS2(ms8)]], liquid chromatography tandem mass spectrometry, LC-MS, CG2043, Sialidase, liquid chromatography tandem mass spectroscopy, Hac-1/Dark, DIWS, Triton X-45, IPP2A2, alphaPS2, NCMe, GBA1, determination, CG 1618, DmelCG6829, Synthetic Detergents, Triton X-305, instrument configuration, Exo-alpha-Sialidase, GLUC, zinc formate, High Performance Liquid Chromatography Mass Spectrometry, lead salt, Poly(oxy-1, Mid(H15), High Performance Liquid Chromatography-Mass Spectrometry, Alkylations, peptide, Detergent, Techniques, Pod, Synthetic Detergent, halite, ammonium tetraformate, FBXO1, peptido, Liquid Chromatography-Mass, DmNSF, DmelCG3428, BANF, Detergents, dNSF1, Octoxynol 9, dNsf1, Min, Williams-Beuren syndrome chromosomal region 6 protein, Apaf1, ARK, Injectables, Effect, DmelCG42273, 3-hydroxy-5-estrane-17-carbonitrile, LCMSMS, kDa, enzymes, peptides, ATP-dependent proteolysis, TAF-I, 2610036I19Rik, 2610510L13Rik, E430039A18Rik, hypoplasia, min, arc, mAPC, copper, ark, Protein Degradation, T1, cloruro sodico, IGAAD, fSAP152, lithium salt, Methodological Studies, DmelCG10574, Liquid Chromatography-Mass Spectrometries, Diws1t, ppm, T18c, TF, CG7776, TEAB, dApaf1, GPHYSD2, ammonium (2:1) salt, Long-Term Effects, 37b, 37c, phapii, rock salt, D-Apaf-1, PS2, LC-MS/MS, Dmel_CG7826, StF-IT-1, Th, Acylneuraminyl hydrolase, L[[3]]CP3, Thermo Scientific, Triton X-100, aPS2, CT27194, HCD, Ionen, Digestion, SRF-Phox1-interacting protein, 4631401G09Rik, WASH, nickel (+2) salt, Dmel_CG7835, Mnb, MNB, dnsf1, parent ion, l(2R)W5, ammonium salt, Pods, acidification, MS/MS, Xylella fastidiosa str. Temecula1, OS3, cobaltous formate, CG9623, hac1, MASS, CG4299, AW124434, natrii chloridum, N-Acylneuraminate, Methodological Study, PLAP-1, Plap1, organ system, dapaf-1S, apaf-1, dapaf-1L, GCB, Agents, precursor ion, Charge, WBS, E(PC), PLAP1, anon-48Ac, copper salt, Biocatalyst, Qed-1, CG8428, Liquid, Polypeptide, PSalpha2, i2pp2a, Procedures, Dapaf-1/HAC-1, Laundry Detergent Pods, Degradation, cesium salt, Integrin, Comt, FBN, ACINUS, TFII-I, PHAPII, LC-MS-MS, hardware, Buffer, Bruton tyrosine kinase-associated protein 135, NSF, Nsf, formic acid, Dark/Hac-1/dApaf1, method, CG1618, Hac1, potassium salt, Octoxynols, Dark/Hac-1/dApaf-1, III, reduced, glycosylation, Octylphenoxy, Glycosylation, ECTOL1, Injection, p63, 14C-labeled, method used in an experiment, p65, Studies, IB291, tiny, Glycohydrolases, AA673430, Pro-Mega, Ultra-Performance Liquid Chromatography-Mass Spectrometry, N Acylneuraminate Glycohydrolases, alpha[[PS2]], iones, MS2, ipp2a2, dapaf-1, los, chlorure de sodium, dapaf, dark, calcium formate, Protein Degradations, Charges, integrin, Xylella fastidiosa (strain Temecula1 / ATCC 700964), OCTD, Fee, Octylphenoxypolyethoxyethanols, 10^[-6], DARK, taf-ibeta, DmelCG1618, Laundry Pod, cromium (+3) salt, Long-Term Effect, AA553322, BTK-associated protein 135, dArk, Chromatography-Mass Spectrometries, Dapaf-1, sodium formate, Laundry, small, DYRK1, data, nickel formate, 3H-labeled, igaad, strontium formate, apaf1, enzyme activity, Protein Digestion, strontium salt, Peptide, group, LC/MS/MS, Oligosaccharide, Protein, I2PP2A, table salt, Dyrk1, 25/11, Dark, connected anatomical system, (3alpha, Dark/Apaf-I, ACETONITRILE, SLRR1C, tandem MS, cyanomethane, WMS2, CG13176, Cleansing, PRSS, Triton X 45, nmr2, betaGC, UNQ215/PRO241, CC1, Tripcellim, anon-35Fa, TBX20, Alphacutanée, dApaf-1/DARK/HAC-1, sample population, chromic formate, inf, l(2)10403, Trypure, N-Acylneuraminate Glycohydrolases, dSET/TAF-Ibeta, 2610030F17Rik, H-2T23, Cleansing Agent, Ion, concentration, anon-35Fc, AA986886, Chromatography-Mass Spectrometry, SSKS, PS2alpha, calcium salt, Qa-1, Peptid, assay, SCH, AA407739, dAPAF-1, 3-OHECNbrown rat, Rat, Rattus norvegicus, alpha[[PS2]], alphaPS2, norvegicus, Laboratory, PS2, Integrin, Norway Rat, CG9623, Rattus, rats, integrin, Norway Rats, Laboratory Rat, inf, jecur, iecur., Livers, aPS2, CT27194, rat, Rattus sp. strain Wistar, in, Mus norvegicus, DmelCG9623, Rats, Rattus norwegicus, alphaPS2C, Mammals, Laboratory Rats, PS 2, PS2alpha, site, alpha[[PS2(ms8)]], Norway rat, Norway, alpha2Int, Rattus rattiscus, PSalpha2, region, If, Rattus norvegicus8, Gunn ratsSH2-Bb, DUbc9, IPP2A2, E 920, ion, GBA1, Dm NinaC, NINA C, cleavage, GLUC, Irip, A4, CG5125, Glykan, protein, NAALAdase, EIC, GCase, 5730420M11Rik, Alkylations, Polypeptides, NAALADase I, protein polypeptide chains, L-Isomer Methionine, polisacarido, FOLH, %, Lwr, Methionine, peptido, C530001K22Rik, 2-amino-3-mercaptopropanoic acid, Cystein, 3.4.17.21, protein aggregate, WMS, C79691, SH2-B, somitogenic mesoderm, SET, C, peptides, Glycans, Membrane glutamate carboxypeptidase, TAF-I, cisteina, M, Glycane, proteins, NAALAD1, Structures, Folylpoly-gamma-glutamate carboxypeptidase, Lccp, DmelCG4299, IGAAD, set, Nina C, DmelCG10574, ppm, Sialic, Glycan, Half Cystine, GPHYSD2, Racemethionine, CT16120, SGS, phapii, Primary Protein Structure, Zinc Cysteinate, unsegmented mesenchyme, rodents, T5E21.12, 2-Amino-4-(methylthio)butyric acid, StF-IT-1, ovalbumin, N-Acetylneuraminic, H(3)0+, SH2-B PH domain-containing signaling mediator 1, not genetically inherited, Cys, DmelCG5125, Dipodidae, ACMICD, Glykane, GIG27, Hydrochaeri, Ionen, PSM, Psm, Methionin, hbl, Amino Acid Sequences., 4631401G09Rik, Hmet, Prostate-specific membrane antigen, presumptive somite mesoderm, (2R)-2-amino-3-sulfanylpropanoic acid, region, l(2)02858, T5E21_12, DRONINAC, parent ion, l(2)05487, Acid, Amino Acid, l(2)05486, Epistemology, Pteroylpoly-gamma-glutamate carboxypeptidase, HLA-DR-associated protein II, DI-2, i56, I-2Dm, OS3, FGCP, oxonium, MASCOT, L-Cysteine, MASS, CG4299, Beaver, GC, i105, L-Cystein, PLAP-1, Plap1, I-2PP1, polysaccharides, N-glycan metabolism, Pro-rich, DMDA, TAF-IBETA, GCB, precursor ion, Rodentias, PLAP1, PSMA, TAF-Ibeta, Polypeptide, i2pp2a, Liquimeth, SH2 domain-containing protein 1B, 2-amino-4-(methylsulfanyl)butanoic acid, Cysteine Hydrochloride, AW549739, sebaceous cyst, unsegmented paraxial mesoderm, L Isomer, L-Zystein, Integrin, FBN, glycans, Gene, Capybara, rodent, precursor, protein-containing complex, somitomeric mesoderm, GCPII, TYPE, PHAPII, DAGA4, dip4, polypeptide chain, glycosylation, Glycosylation, ECTOL1, Gene Products, L-Methionine, N-acetylated-alpha-linked acidic dipeptidase I, Ubc 9, mKIAA1299, Half-Cystine, NINAC, MAM, SCG3, Pedameth, CG54125, Polysaccharide, Slrr1c, SH2B, NinaC, ninac, DmelCG3018, L Cysteine, iones, E-920, ipp2a2, 2pp2a, N-glycan biosynthesis, mGCP, Castor Beaver, Hcys, Ubc-9, ions, semi, Structure, CG10574, CG 5125, l(2)01519, OCTD, mKIAA0989, 2PP2A, 2-amino-4-(methylthio)butanoic acid, 10^[-6], CYSTEINE, taf-ibeta, Protein Structure, Jerboas, dSET, dSet, FREE CYSTEINE, site, peptidos, 2.2, (2R)-2-amino-3-mercaptopropanoic acid, Primary Protein, metionina, Hydrochaeris, data, UBC9, lumen, Protein Glycosylation, DL-Methionine, Ubc9, protein complex, space, Proteins, Sialic Acid, igaad, Primary, L-Isomer, Peptide, group, LGMD2C, GCP2, Glutamate carboxypeptidase II, Sequences, N Acetylneuraminic Acid, Cell growth-inhibiting gene 27 protein, native protein, FBgn0010602, natural protein, I-2PP2A, Sequence, Protein, Dm I-2, dUbc9, I2PP2A, dUBC9, CT42491, MFS1, ubc9, segmental plate, anatomical spaces, Protein Structures, Mopsm, Sh2bpsm1, Zystein, SLRR1C, Data Base, WMS2, Met, polisacaridos, lumen space, PH and SH2 domain-containing signaling mediator, DMDA1, ensemble, Beavers, 2-amino-3-sulfanylpropanoic acid, betaGC, UNQ215/PRO241, Aspn, L-2-Amino-3-mercaptopropionic acid, Rodents, Jerboa, (R)-2-amino-3-mercaptopropanoic acid, Capybaras, Rodent, 2-Amino-3-mercaptopropionic acid, Folate hydrolase 1, Protein Gene Products, Gene Proteins, dSET/TAF-Ibeta, 2610030F17Rik, Ion, AA986886, Dmubc9, SSKS, SCARMD2, AI425885, alpha-amino-gamma-methylmercaptobutyric acid, E920, Peptid, epidermoid cyst, Primary Protein Structures, AA407739, CG3018, DmUbc9falseSite-specific N-glycolylation profiles in a5b1 integrin from rat liverTo visualize glycosylation in the context of the integrin structure, the quantitative site-specific glycosylation profiles of a5b1 integrin were determined and the main representative glycans were mapped onto a homology model of rat a5b1 integrin in its bent-closed inactive conformation.2024-07-252022-01-30PXD031334NEWT:330879NEWT:377960NEWT:1131NEWT:2042546NEWT:259447NEWT:295546NEWT:1334565NEWT:625351NEWT:258594NCBITaxon:1280NEWT:112503NEWT:1129NEWT:309807NEWT:59753NEWT:89184NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:193516NEWT:1073882NEWT:6819NEWT:475174NEWT:1117NEWT:498257NEWT:10036NEWT:1590NEWT:10034NEWT:33114NEWT:661410NEWT:638632NEWT:224326NEWT:376619NCBITaxon:79857NEWT:1096976NEWT:3765NEWT:3767NEWT:1589NEWT:135622NEWT:672127NEWT:35786NEWT:1580NEWT:399784NEWT:96731NEWT:656064NEWT:1582NEWT:1584NEWT:36630NEWT:1585NEWT:383379NCBITaxon:12721NEWT:10029NEWT:913645NEWT:641809NEWT:1392488NEWT:38815NEWT:317447NEWT:4688NEWT:7719NEWT:5530NEWT:868565NEWT:135674NEWT:79329NEWT:30069NEWT:12637NEWT:59729NEWT:2164133NEWT:295105NEWT:108061NEWT:60711NEWT:224308NEWT:3347NEWT:160621NEWT:212790NEWT:110368NEWT:1310161NEWT:77133NEWT:145481NEWT:1310165NEWT:29058NCBITaxon:79824NEWT:410658NEWT:5516NEWT:44688NEWT:44689NEWT:498211NEWT:347256NEWT:5518NEWT:398007NEWT:1527468NEWT:498217NEWT:498216NEWT:11320NEWT:246196NEWT:246197NEWT:145458NEWT:1310173NEWT:44685NEWT:161934NEWT:1148NEWT:5508NEWT:3329NEWT:419481NEWT:5507NEWT:410661NEWT:1140NCBITaxon:2157NEWT:1143NEWT:1287689NEWT:1094343NEWT:1462472NEWT:1336795NEWT:644042NEWT:150808NEWT:1182590NEWT:3712NEWT:3711NEWT:3714NCBITaxon:37162NEWT:2065263NEWT:177437NEWT:10891NEWT:10418NEWT:118698NEWT:1616117NEWT:118696NEWT:34865NEWT:52283NEWT:284812NEWT:8175NEWT:43330NEWT:1603293NEWT:44664NEWT:43346NEWT:3702NEWT:1245466NEWT:3704NEWT:244366NEWT:1246791NEWT:176597NEWT:118694NEWT:2850NEWT:118691NEWT:34871NEWT:33548NEWT:52299NEWT:8165NEWT:96794NEWT:3708NEWT:332648NEWT:44670NEWT:926571NEWT:536231NEWT:376219NEWT:219813NEWT:1510NEWT:460519NEWT:1515NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:1348799NEWT:33995NEWT:55784NEWT:1303443NEWT:485NEWT:56636NEWT:2499525NEWT:2853422NEWT:487NEWT:1538553NEWT:1679718NEWT:480NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:1185650NEWT:216778NEWT:1502NEWT:128017NEWT:376686NEWT:237610NEWT:95486NEWT:119562NEWT:1883446NEWT:1233435NEWT:118223NEWT:11757NEWT:987059NEWT:109760NEWT:29031NEWT:317047NEWT:491NEWT:460523NEWT:45954NEWT:235443NEWT:1757312NEWT:85694NEWT:109786NEWT:108458NEWT:5936NEWT:320637NEWT:1078283NEWT:3750NEWT:983964NEWT:11706NEWT:32644NEWT:527796NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:3747NEWT:1116234NEWT:1255228NEWT:410289NEWT:373153NEWT:1640278NEWT:472NEWT:940825NEWT:529507NEWT:1071661NEWT:28128NEWT:470NEWT:5911NEWT:748693NCBITaxon:50557NEWT:39251NEWT:29491NEWT:101841NEWT:446NEWT:153481NEWT:2014887NEWT:33952NEWT:445NEWT:153009NEWT:261756NEWT:166313NEWT:63366NEWT:63367NEWT:215402NEWT:1547NEWT:1290454NEWT:906914NEWT:28141NEWT:9031NEWT:27292NEWT:1050722NEWT:3728NEWT:108931NEWT:1293497NEWT:1055524NEWT:150475NEWT:267872NEWT:381666NEWT:172269NEWT:9534NEWT:5180NEWT:256737NEWT:9541NEWT:8694NEWT:33936NEWT:8692NEWT:2903NEWT:185579NEWT:13076NEWT:207340NEWT:1006581NEWT:33940NEWT:550NEWT:554NEWT:451516NEWT:1325291NEWT:42410NEWT:36185NEWT:1054211NEWT:1225786NEWT:771870NEWT:51338NEWT:575412NEWT:28112NEWT:49012NEWT:6493NEWT:10832NEWT:6494NEWT:6491NEWT:507601NEWT:520NEWT:186441NEWT:643680NEWT:214092NCBITaxon:6157NEWT:13095NEWT:162425NEWT:104105NEWT:216257NEWT:9986NEWT:8654NEWT:8658NEWT:1268063NEWT:8657NEWT:9983NEWT:8655NEWT:5147NEWT:28108NEWT:28104NEWT:407821NEWT:568703NCBITaxon:2NEWT:568708NEWT:1613NEWT:986NEWT:52641NEWT:28532NEWT:353152NEWT:298176NEWT:353153NEWT:227377NEWT:1774284NEWT:40674NEWT:1194669NEWT:443906NEWT:13037NEWT:519NEWT:2510939NEWT:7394NEWT:6063NEWT:1328388NEWT:105487NEWT:1548728NEWT:667127NEWT:9557NEWT:377586NEWT:300641NEWT:39655NEWT:9554NEWT:38323NEWT:475932NEWT:256318NEWT:206411NCBITaxon:6191NEWT:229533NEWT:214053NEWT:28550NEWT:80863NEWT:90675NEWT:52639NEWT:52638NEWT:57075NEWT:1666905NEWT:8697NEWT:861109NEWT:8695NEWT:884204NEWT:1045775NEWT:1123869NEWT:9544NEWT:9545NEWT:979NEWT:7370NEWT:83906NEWT:99488NEWT:1134506NEWT:255470NEWT:38783NEWT:6426NEWT:33090NEWT:9935NEWT:287889NEWT:305959NEWT:1028307NEWT:13489NEWT:29829NEWT:92867NEWT:92866NCBITaxon:3055NEWT:51750NEWT:202950NEWT:441772NEWT:1293091NEWT:48106NEWT:202956NEWT:295027NCBITaxon:11320NEWT:632957NEWT:9925NCBITaxon:9606NEWT:90690NEWT:1436183NEWT:300273NEWT:4232NEWT:416348NEWT:11298NEWT:4236NEWT:7742NEWT:196627NEWT:1076878NEWT:200308NEWT:200302NEWT:870435NEWT:58370NEWT:9913NEWT:9915NEWT:105841NEWT:2666255NEWT:999810NCBITaxon:5693NEWT:28995NEWT:2144189NEWT:1392998NEWT:380394NEWT:1150621NEWT:226900NEWT:1266738NEWT:231490NEWT:244704NEWT:7725NEWT:9909NEWT:9902NEWT:430615NEWT:72664NEWT:563041NEWT:36599NEWT:41949NEWT:249161NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1678078NEWT:749906NEWT:418985NEWT:749907NEWT:150847NEWT:29835NEWT:431947NEWT:69014NEWT:142809NEWT:1095183NEWT:302409NEWT:130821NEWT:27606NEWT:1519788NEWT:59202NEWT:9975NEWT:8643NEWT:1801631NEWT:1159899NEWT:502780NEWT:860688NEWT:13443NEWT:35231NEWT:1774208NEWT:8644NEWT:1125630NEWT:5141NEWT:46360NCBITaxon:3044782NEWT:202914NEWT:160235NEWT:502779NEWT:297246NEWT:103690NEWT:8639NEWT:1928434NEWT:8637NEWT:1114970NEWT:2096NEWT:285272NEWT:192222NEWT:9963NEWT:1617910NEWT:197911NEWT:197912NEWT:637379NEWT:12107NEWT:2785785NEWT:411901NEWT:1298722NEWT:8626NEWT:186801NEWT:105884NCBITaxon:9615NEWT:8621NEWT:58334NEWT:58331NEWT:6446NEWT:8624NEWT:5116NEWT:6447NEWT:8623NEWT:5597NEWT:194439NCBITaxon:12637NEWT:38727NEWT:218496NEWT:8618NEWT:70448NEWT:300251NEWT:9940NEWT:13451NEWT:1247190NEWT:118702NEWT:118700NEWT:2126719NEWT:118705NEWT:6945NEWT:2104NEWT:2627626NEWT:6943NEWT:157546NEWT:91374NEWT:91373NEWT:1414448NEWT:474186NEWT:54195NEWT:10136NEWT:38942NEWT:4762NEWT:6941NEWT:307972NEWT:2102NEWT:34NEWT:38949NEWT:1299114NEWT:1590841NEWT:60876NEWT:1276473NEWT:2282334NEWT:1247NEWT:70667NEWT:869728NEWT:6938NCBITaxon:39054NEWT:1736309NEWT:4751NEWT:36774NEWT:194963NEWT:77298NEWT:15819NEWT:109461NEWT:1085956NEWT:6928NEWT:1239NEWT:36745NEWT:400727NEWT:272560NEWT:272563NEWT:11009NEWT:913998NEWT:68335NEWT:3885NEWT:3888NEWT:649408NEWT:6915NEWT:101510NEWT:3880NEWT:272559NEWT:216816NEWT:153151NEWT:515849NEWT:10141NEWT:95767NEWT:466585NEWT:1000589NEWT:85963NEWT:85962NEWT:42197NEWT:6918NEWT:681196NEWT:143361NEWT:5656NEWT:1292NEWT:1299NEWT:316407NEWT:1794699NCBITaxon:183674NEWT:167387NEWT:84645NEWT:1123294NEWT:6978NEWT:209285NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:1000561NEWT:253836NEWT:2133NEWT:6973NEWT:294381NEWT:197NEWT:1390363NEWT:35889NEWT:406072NEWT:351660NEWT:29176NEWT:201319NEWT:1073569NEWT:4787NEWT:751585NEWT:113549NEWT:180788NEWT:159749NEWT:1270NEWT:388919NEWT:130081NEWT:4784NEWT:360106NEWT:4785NEWT:360104NEWT:10117NEWT:401614NEWT:10116NEWT:1280NEWT:83333NEWT:83334NEWT:242718NEWT:83332NEWT:161NEWT:242715NEWT:2951803NEWT:100226NEWT:1263NEWT:176299NEWT:1654360NEWT:54126NEWT:75058NEWT:13616NEWT:13613NEWT:296543NEWT:173NEWT:316435NEWT:42157NEWT:1034304NEWT:105023NEWT:866628NEWT:535026NEWT:749200NEWT:1036967NEWT:266264NEWT:272942NEWT:272943NEWT:1882682NEWT:145953NEWT:257309NEWT:33657NEWT:203124NEWT:230741NEWT:8296NEWT:117008NEWT:167748NEWT:645463NEWT:749696NEWT:320549NEWT:990119NEWT:77216NEWT:1111708NEWT:11855NEWT:998086NEWT:228410NEWT:493760NEWT:93163NEWT:257313NEWT:3827NEWT:400772NEWT:257310NEWT:128161NEWT:930945NEWT:332521NEWT:106350NEWT:29170NEWT:269796NEWT:54571NEWT:412754NEWT:9598NEWT:52389NEWT:2665964NEWT:1423772NEWT:51056NEWT:35815NEWT:53232NEWT:1235987NEWT:1639NEWT:188229NEWT:3818NEWT:432359NEWT:9103NEWT:715877NEWT:862238NEWT:29159NEWT:1155748NEWT:9593NEWT:8260NEWT:9595NEWT:2665958NEWT:246200NEWT:6087NEWT:145943NEWT:595536NEWT:240906NEWT:89920NEWT:3809NEWT:29158NEWT:44744NEWT:3879NEWT:1328881NEWT:411483NEWT:76336NEWT:3870NEWT:884019NEWT:411490NEWT:169963NEWT:587NEWT:588NEWT:585NEWT:746128NEWT:212035NCBITaxon:1653394NEWT:1205NEWT:214693NEWT:45609NEWT:239935NEWT:1680NEWT:280148NEWT:9172NEWT:2770979NEWT:112NEWT:423477NEWT:1277351NEWT:1594786NEWT:40366NEWT:3854NEWT:411469NEWT:1679NEWT:333849NEWT:214684NEWT:1758543NEWT:569NEWT:411464NEWT:411460NEWT:1328426NEWT:562NEWT:411470NEWT:10515NEWT:2094720NCBITaxon:2697049NEWT:294750NEWT:1663NEWT:214675NEWT:62141NEWT:65651NEWT:3848NEWT:3847NEWT:198466NEWT:327159NEWT:1045010NEWT:178876NEWT:1660NEWT:327160NEWT:573NEWT:1131757NEWT:571NEWT:142479NEWT:29595NEWT:241368NEWT:42528NEWT:1969471NEWT:9657NEWT:150596NEWT:30540NEWT:439733NEWT:76777NEWT:30545NEWT:76773NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:1504802NEWT:1821314NEWT:103351NEWT:1249668NEWT:426430NEWT:37181NEWT:929439NEWT:9646NEWT:5286NEWT:7469NEWT:9644NEWT:426428NEWT:63459NEWT:2652157NEWT:2652158NEWT:40353NEWT:40324NEWT:588858NEWT:9638NEWT:9639NCBITaxon:11071NEWT:693977NEWT:242231NEWT:197221NEWT:528354NEWT:51453NEWT:1279085NEWT:28231NEWT:7460NEWT:504457NEWT:392227NEWT:1004024NEWT:103372NEWT:862751NEWT:470150NEWT:9627NEWT:1009714NEWT:73239NEWT:38018NEWT:226186NEWT:381518NEWT:30532NEWT:8782NEWT:115357NEWT:5270NEWT:63436NEWT:63433NEWT:13164NEWT:1601067NEWT:129554NEWT:40302NEWT:1249615NEWT:343232NEWT:343237NEWT:2664179NEWT:623NEWT:217219NEWT:7038NEWT:6192NCBITaxon:1639NEWT:3915NEWT:1581419NEWT:405534NEWT:9689NEWT:8355NEWT:633NEWT:9685NEWT:1218948NEWT:7029NEWT:9688NEWT:6182NEWT:9694NEWT:604162NEWT:1046723NEWT:186990NEWT:8364NEWT:6185NEWT:632NEWT:6183NEWT:1423800NEWT:630NCBITaxon:11019NEWT:334747NEWT:3907NEWT:3906NEWT:27315NEWT:61235NEWT:343691NEWT:1457551NEWT:15368NEWT:13180NEWT:1491466NEWT:340188NEWT:170187NEWT:1755437NEWT:1042399NEWT:9681NEWT:1718NEWT:1719NEWT:692672NEWT:2697049NEWT:568813NEWT:267566NEWT:58042NEWT:7004NEWT:9669NCBITaxon:6073NEWT:1089448NEWT:1329349NEWT:1089444NEWT:607699NEWT:6161NEWT:206542NEWT:27337NEWT:575584NEWT:186536NEWT:257771NEWT:185687NEWT:3039NEWT:2706014NEWT:8727NEWT:8726NEWT:8725NEWT:10090NEWT:8729NEWT:8720NEWT:5693NEWT:2186NEWT:8724NEWT:8723NEWT:283166NEWT:439334NEWT:8722NEWT:5697NEWT:6544NEWT:218192NCBITaxon:10114NEWT:1685931NEWT:242619NEWT:557722NEWT:160791NEWT:1353008NEWT:34073NEWT:402042NEWT:373995NEWT:8715NEWT:5689NEWT:544404NEWT:287535NEWT:2173NEWT:2175NEWT:1232801NEWT:5691NEWT:61674NEWT:2072580NEWT:669465NEWT:1117957NEWT:6526NEWT:8709NEWT:1064539NEWT:8707NEWT:1306274NEWT:5671NEWT:13146NEWT:6523NEWT:8700NEWT:1692259NEWT:1486917NEWT:1843183NEWT:5661NEWT:157592NEWT:3482NEWT:52714NEWT:51866NEWT:58002NEWT:13136NEWT:5660NEWT:5665NEWT:1179777NEWT:6997NEWT:5666NEWT:1746091NEWT:5664NEWT:2157NEWT:70125NEWT:146479NEWT:905079NEWT:1843192NEWT:206189NEWT:2160NEWT:295139NEWT:27779NEWT:181214NEWT:6108NEWT:1720309NEWT:9612NEWT:1286640NEWT:38865NEWT:3077NEWT:9615NEWT:6100NEWT:713604NEWT:3076NEWT:112273NEWT:33178NEWT:1147787NEWT:39717NEWT:62977NEWT:2562269NEWT:1440772NEWT:8759NEWT:9605NEWT:9606NEWT:27717NEWT:641501NEWT:9601NEWT:8752NEWT:34038NEWT:9604NEWT:8756NEWT:74940NEWT:13551NEWT:10041NEWT:9600NEWT:1450511NEWT:439375NEWT:1857323NEWT:890382NEWT:174934NEWT:179392NEWT:2823525NEWT:115778NEWT:1538186NEWT:82996NEWT:227086NEWT:3055NEWT:195883NEWT:1450520NEWT:1042311NEWT:1123352NEWT:741258NEWT:82525NEWT:29920NEWT:235909NEWT:47311NCBITaxon:813NEWT:127785NEWT:708248NEWT:47314NEWT:104660NEWT:652611NEWT:94993NEWT:8732NEWT:763037NEWT:1250232NEWT:1052588NEWT:1377NEWT:3555NEWT:71647NEWT:260201NEWT:35554NCBITaxon:12939NEWT:935692NEWT:32046NEWT:2222NEWT:59523NEWT:544496NEWT:59529NEWT:32049NEWT:1392696NEWT:762376NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:3544NEWT:5722NEWT:7901NEWT:32051NEWT:10245NEWT:55153NEWT:10243NEWT:351581NEWT:43186NEWT:428146NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:32025NEWT:2649997NEWT:1147161NEWT:43150NEWT:69781NEWT:85831NEWT:263737NEWT:1333499NEWT:5702NEWT:95648NEWT:1349NEWT:100787NEWT:1401257NEWT:44491NEWT:27933NEWT:556484NEWT:7955NEWT:7959NEWT:645159NEWT:2261NEWT:31156NEWT:3112NEWT:8801NEWT:4442NEWT:192875NEWT:1214916NEWT:284218NEWT:1316931NEWT:45796NEWT:511145NEWT:213401NEWT:931281NEWT:4432NEWT:5762NEWT:5763NEWT:118099NEWT:32019NEWT:658457NEWT:420247NEWT:35525NEWT:295358NEWT:2246NEWT:2652724NEWT:5757NEWT:68415NCBITaxon:4563NEWT:5755NEWT:44447NEWT:752555NEWT:39097NEWT:347495NEWT:5759NEWT:1736231NEWT:1392NEWT:35974NEWT:2242NEWT:1396NEWT:286NEWT:391619NEWT:287NEWT:633149NEWT:1149786NEWT:10239NEWT:68895NEWT:3567NEWT:68888NEWT:4897NEWT:5744NEWT:72901NEWT:213863NEWT:55571NEWT:35500NEWT:2041044NEWT:3562NEWT:4896NEWT:5740NEWT:1262161NEWT:1314781NEWT:1390NEWT:11557NEWT:294NEWT:1773NEWT:82380NEWT:935293NEWT:375146NEWT:471876NEWT:1772NEWT:263NEWT:11988NEWT:400667NEWT:408169NEWT:36809NEWT:47946NEWT:105135NEWT:1764NEWT:39087NEWT:413996NEWT:243277NEWT:7067NEWT:586722NEWT:243273NEWT:1986198NEWT:274NEWT:7070NEWT:408172NEWT:408170NEWT:1436733NEWT:42229NEWT:1247411NEWT:9238NEWT:13708NEWT:333760NEWT:34607NEWT:1513458NEWT:1233681NEWT:2212966NCBITaxon:40559NEWT:506599NEWT:81077NEWT:84588NEWT:1279007NEWT:85433NEWT:65349NEWT:300852NEWT:564933NEWT:508771NEWT:1121405NEWT:253NCBITaxon:2759NEWT:93061NEWT:93062NEWT:1284813NEWT:34613NEWT:7054NCBITaxon:4932NEWT:1748NEWT:108673NEWT:2764168NEWT:943274NEWT:1488406NEWT:644223NEWT:1263082NEWT:272623NEWT:1288389NEWT:272624NEWT:886559NEWT:55521NEWT:118499NEWT:1130798NEWT:55529NEWT:256136NEWT:476272NEWT:195051NEWT:3988NEWT:561007NEWT:649908NEWT:1001135NEWT:147828NEWT:3981NEWT:3983NEWT:203696NEWT:352472NEWT:235NEWT:505235NEWT:55518NEWT:97441NEWT:1154756NEWT:1202531NEWT:1202532NEWT:360094NEWT:1408223NEWT:1408224NEWT:41364NEWT:1311NEWT:1313NEWT:1218097NEWT:1080348NEWT:1314NEWT:39491NEWT:212142NCBITaxon:5811NEWT:3972NEWT:1499105NEWT:686NEWT:192812NEWT:408188NEWT:571256NEWT:40483NEWT:688333NEWT:1119529NEWT:1303NCBITaxon:548681NEWT:318586NCBITaxon:5820NEWT:272634NEWT:198107NEWT:1979401NEWT:155892NEWT:691NEWT:28384NEWT:167879NEWT:1872122NEWT:1308NEWT:7091NEWT:1034015NEWT:760568NEWT:141262NEWT:1813NEWT:4087NEWT:9778NEWT:306NEWT:303NEWT:97477NEWT:7111NEWT:113287NEWT:347515NEWT:6269NEWT:4090NEWT:4092NEWT:4093NEWT:786NEWT:4096NEWT:208895NEWT:941442NEWT:536088NEWT:311553NEWT:1226408NEWT:43984NEWT:7108NEWT:40479NEWT:43989NEWT:4076NEWT:142615NEWT:317NEWT:885319NEWT:293939NEWT:497515NEWT:885318NEWT:39488NEWT:4081NEWT:273068NEWT:1316582NEWT:227166NEWT:98334NEWT:231663NEWT:4084NEWT:63577NEWT:7574NEWT:1715256NEWT:30640NEWT:348824NEWT:929793NEWT:1507179NEWT:29204NEWT:114155NEWT:4072NEWT:1337056NEWT:4073NEWT:634955NEWT:6239NEWT:102169NEWT:208893NEWT:4054NEWT:301301NEWT:98351NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:63558NEWT:61376NEWT:207559NEWT:107401NEWT:85057NEWT:985076NEWT:1215323NCBITaxon:565995NEWT:7159NEWT:267671NEWT:72491NEWT:7160NEWT:8496NEWT:7165NEWT:1257118NEWT:1387333NEWT:8010NEWT:1315282NEWT:7162NEWT:1859NEWT:27448NEWT:2708NEWT:38111NEWT:235612NEWT:243232NEWT:243230NEWT:936053NEWT:1080772NEWT:8479NEWT:8485NEWT:119856NEWT:72000NEWT:119857NEWT:49240NEWT:161398NEWT:2893330NCBITaxon:3707NEWT:391295NEWT:1170828NEWT:93934NEWT:9315NEWT:6289NEWT:38568NEWT:6287NEWT:38569NEWT:727NEWT:9796NEWT:725NEWT:7137NEWT:469008NEWT:9321NEWT:596153NEWT:1836NCBITaxon:10298NEWT:1652998NEWT:105231NEWT:104385NEWT:74649NEWT:1037924NEWT:206672NEWT:9788NEWT:4097NEWT:9305NEWT:436113NEWT:71139NEWT:9785NEWT:6279NEWT:1195774NEWT:9793NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:27457NEWT:4006NEWT:1872635NCBITaxon:5476NEWT:113208NEWT:6669NEWT:1215360NEWT:89453NEWT:1114301NEWT:51515NEWT:13249NEWT:588596NEWT:51511NEWT:8845NEWT:7998NEWT:419610NEWT:5334NEWT:382352NEWT:413071NCBITaxon:10239NEWT:332097NCBITaxon:714NEWT:1076620NEWT:11051NEWT:89462NEWT:452680NEWT:8839NEWT:4472NEWT:2758385NEWT:171101NEWT:714NEWT:5322NEWT:568996NEWT:421932NEWT:312017NEWT:576131NEWT:553470NCBITaxon:10243NEWT:284593NEWT:100392NEWT:655819NEWT:5315NEWT:11082NEWT:265872NEWT:59463NEWT:337330NEWT:2285NEWT:2287NEWT:6645NEWT:251229NEWT:981087NEWT:180066NEWT:46147NCBITaxon:6312NEWT:11072NEWT:174633NEWT:52830NEWT:1416333NEWT:35024NEWT:135461NEWT:7962NEWT:99287NEWT:1817808NEWT:145262NEWT:145263NEWT:296587NEWT:694569NEWT:9739NEWT:111069NEWT:1379791NEWT:51953NEWT:57267NEWT:3197NEWT:13686NEWT:2750454NEWT:264561NEWT:1402135NEWT:36329NEWT:62615NEWT:1555636NEWT:1333550NEWT:646099NEWT:72407NEWT:352914NEWT:349741NEWT:409859NEWT:157295NEWT:1263871NEWT:509169NEWT:6210NEWT:9721NEWT:137221NEWT:565050NEWT:409860NEWT:92683NEWT:1298968NEWT:5353NEWT:1034809NEWT:452659NEWT:1193501NEWT:51930NEWT:1193502NEWT:6689NEWT:194675NEWT:298315NEWT:392547NEWT:46105NEWT:104782NEWT:10181NEWT:9704NEWT:1049056NEWT:698936NEWT:13216NEWT:1009874NEWT:4498NEWT:8855NEWT:11034NEWT:3168NEWT:10185NEWT:78168NEWT:521001NEWT:192011NEWT:1813887NEWT:224911NEWT:1090946NCBITaxon:11552NEWT:267608NEWT:334249NEWT:184922NEWT:6703NEWT:2NEWT:35670NEWT:5855NEWT:4528NEWT:35677NEWT:136841NEWT:1493NEWT:10376NEWT:1496NEWT:3674NEWT:5851NEWT:660469NEWT:935568NEWT:1365186NEWT:1813019NEWT:45474NEWT:48500NEWT:4513NEWT:5843NEWT:110205NEWT:1117645NCBITaxon:10407NEWT:1000364NEWT:10360NEWT:1403536NEWT:1427524NEWT:47664NEWT:260799NEWT:317548NEWT:106198NEWT:2323NEWT:3654NEWT:237561NEWT:5833NEWT:3659NEWT:59620NEWT:499229NEWT:31298NEWT:1204162NEWT:1532734NEWT:82688NEWT:5823NEWT:5821NEWT:44139NCBITaxon:4895NEWT:570417NCBITaxon:4896NEWT:675512NEWT:315393NEWT:3649NEWT:28903NEWT:910263NEWT:28909NEWT:3641NEWT:79698NCBITaxon:5796NEWT:12993NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:170721NEWT:34305NEWT:77020NEWT:1908323NEWT:83558NEWT:44586NEWT:626528NEWT:139927NEWT:4558NEWT:5888NEWT:431241NEWT:4550NEWT:4555NEWT:443226NEWT:144036NEWT:144030NEWT:288705NEWT:392815NEWT:3217NEWT:4547NEWT:3218NEWT:556182NEWT:374990NEWT:36941NEWT:1432059NEWT:154293NEWT:156471NEWT:3694NEWT:156476NEWT:4543NEWT:210007NEWT:1857017NEWT:10359NEWT:1358430NEWT:10358NEWT:1381693NEWT:1735272NEWT:109298NEWT:317513NEWT:707678NEWT:580240NEWT:294128NEWT:11676NEWT:1917265NEWT:55210NEWT:718308NEWT:4530NEWT:5861NEWT:44577NEWT:1895NEWT:2558360NEWT:64152NEWT:4924NEWT:2748NEWT:8049NEWT:33892NEWT:99931NEWT:9378NEWT:990346NEWT:100816NEWT:192952NEWT:1640346NCBITaxon:1313NEWT:29385NEWT:44544NEWT:529818NEWT:400540NEWT:382NEWT:4911NEWT:152480NEWT:129249NEWT:1888NEWT:1077286NEWT:943129NEWT:131567NEWT:1221594NEWT:10760NEWT:1524260NEWT:227321NEWT:492670NEWT:281677NEWT:106590NEWT:260710NEWT:4918NEWT:1162296NEWT:106592NEWT:596645NEWT:46704NEWT:42345NEWT:482428NEWT:1187947NEWT:4903NEWT:10312NCBITaxon:1773NEWT:323848NEWT:190304NEWT:1432499NEWT:8030NEWT:246432NEWT:246437NEWT:36909NEWT:4909NEWT:1279365NEWT:759272NEWT:1182263NEWT:943146NEWT:646532NEWT:2711NEWT:537457NEWT:479249NEWT:435623NEWT:33889NEWT:10306NEWT:12967NEWT:8022NEWT:42353NEWT:593117NEWT:3633NEWT:319761NEWT:3635NEWT:5811NEWT:86662NEWT:180923NEWT:1451NEWT:198215NEWT:200298NEWT:12916NEWT:1400870NEWT:343NEWT:84005NEWT:225117NEWT:34708NEWT:8090NEWT:5801NEWT:43665NEWT:5802NEWT:4952NEWT:1266464NEWT:367830NEWT:1093790NEWT:178616NEWT:95145NEWT:358NEWT:375451NEWT:54788NEWT:357NEWT:74381NEWT:2010198NCBITaxon:186538NEWT:457428NEWT:316275NEWT:84023NEWT:589904NEWT:559292NEWT:316273NEWT:2762NEWT:1174673NEWT:28035NEWT:1313166NEWT:690307NCBITaxon:138NEWT:28038NEWT:1422NEWT:84030NEWT:1423NEWT:4932NEWT:1205903NEWT:3603NEWT:2756NEWT:2759NEWT:38293NEWT:1428NEWT:339NEWT:2751NEWT:330NEWT:139077NEWT:391904NEWT:12922NEWT:316277NEWT:163159NEWT:1306413NEWT:2813599NEWT:518766NEWT:2664373NEWT:190802NEWT:1213463NEWT:7234NEWT:9417NEWT:1216979NEWT:9895NEWT:7237NEWT:5059NEWT:31631NEWT:509924NEWT:28450NEWT:115104NEWT:5061NEWT:7240NEWT:8570NEWT:127569NEWT:5062NEWT:1408434NEWT:1241935NEWT:220668NEWT:9407NEWT:7227NEWT:193079NEWT:9402NEWT:415540NEWT:336722NEWT:675060NEWT:416870NEWT:334542NEWT:313625NEWT:51240NEWT:27592NEWT:5052NEWT:227289NEWT:1985738NEWT:7215NEWT:196139NEWT:5037NEWT:651182NEWT:2172103NCBITaxon:59201NEWT:7220NEWT:1876254NEWT:434271NEWT:1915NEWT:747679NEWT:66712NEWT:42752NEWT:746360NEWT:89764NEWT:543734NEWT:49390NEWT:9870NEWT:256648NEWT:435590NEWT:1902NEWT:208526NEWT:1908NEWT:1169740NEWT:217459NEWT:216129NEWT:1245012NEWT:171250NEWT:528135NEWT:1637254NCBITaxon:38727NEWT:176946NEWT:74790NEWT:254472NEWT:2829NEWT:126279NEWT:1228997NEWT:1428006NEWT:62335NEWT:366581NEWT:757417NEWT:216599NEWT:216595NEWT:40559NEWT:51208NEWT:216597NEWT:42736NEWT:1607839NEWT:1283300NEWT:1218707NEWT:1204551NEWT:99586NEWT:228577NEWT:2060328NEWT:48038NEWT:392021NEWT:436486NEWT:51655NEWT:1933298NEWT:260707NEWT:121845NEWT:8595NEWT:7263NEWT:186763NEWT:447135NEWT:185431NEWT:216591NEWT:29760NEWT:260704NEWT:703612NEWT:260705NEWT:1295743NEWT:546805NEWT:1505907NEWT:1249885NEWT:853NEWT:7244NEWT:1415167NEWT:36036NCBITaxon:662NEWT:9430NEWT:851NEWT:71251NEWT:208963NEWT:208964NEWT:181088NEWT:48498NEWT:669202NEWT:1193806NEWT:6306NEWT:4120NEWT:37334NCBITaxon:10357NEWT:2040765NCBITaxon:10358NCBITaxon:10359NEWT:1203258NEWT:49317NEWT:1899318NEWT:696811NEWT:9808NEWT:839NEWT:9803NEWT:1097670NEWT:92519NEWT:943925NEWT:4111NEWT:37349NEWT:4113NEWT:837NEWT:640459NEWT:72568NEWT:51641NEWT:35168NEWT:81866NEWT:334147NEWT:1262469NEWT:555778NEWT:277944NEWT:48479NEWT:61435NEWT:627025NEWT:1097677NEWT:375939NEWT:712938NEWT:3258NEWT:112018NEWT:1906951NEWT:101364NEWT:52945NEWT:4100NEWT:1159556NEWT:1076NEWT:4102NEWT:1028688NEWT:4101NEWT:6763NEWT:803NEWT:29722NEWT:37319NEWT:419947NEWT:2847306NEWT:468911NEWT:13373NEWT:266834NEWT:7604NEWT:6756NEWT:817NEWT:818NEWT:135588NEWT:1061NEWT:4571NEWT:29719NEWT:6754NEWT:452589NEWT:509068NEWT:2794344NEWT:4577NEWT:813NEWT:1365218NEWT:337677NEWT:1042986NEWT:1911079NEWT:69373NEWT:427577NEWT:264203NEWT:1480154NEWT:9858NEWT:9859NEWT:512623NEWT:1274414NEWT:2907833NEWT:38626NEWT:10298NEWT:10299NEWT:1087439NEWT:9852NEWT:9860NEWT:660025NEWT:61408NEWT:1355477NEWT:90371NEWT:55194NEWT:2907829NEWT:4153NEWT:11137NEWT:278839NEWT:59552NEWT:35128NEWT:39966NEWT:7668NEWT:58228NEWT:915099NEWT:1143193NEWT:681376NEWT:687914NEWT:1122233NEWT:859313NEWT:59540NEWT:9838NEWT:9839NEWT:546414NEWT:11167NEWT:9835NEWT:7650NEWT:4146NEWT:5478NEWT:6326NEWT:7656NEWT:5476NEWT:5477NEWT:1882945NEWT:746707NEWT:299767NEWT:28835NEWT:1206781NEWT:5480NEWT:1274432NEWT:222863NEWT:2053667NEWT:859321NEWT:1274426NEWT:9825NEWT:1274423NEWT:393765NEWT:104421NEWT:39946NEWT:9823NEWT:95602NEWT:6313NEWT:39947NEWT:1274420NEWT:859314NEWT:578458NEWT:60520