Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD031716/PCF-CM-4806--08--2022_protein_all_PD1.xlsxftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD031716/LUM2_1023541.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD031716/LUM2_1023542.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD031716/LUM2_1023543.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD031716/LUM2_1023545.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD031716/LUM2_1023544.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD031716/LUM2_1023546.rawprimary200OKprashant.mishra@utsouthwestern.eduNicholas LesnerMass SpectrometryGel-based experimentHumanMitochondriaLiverhttps://www.ebi.ac.uk/pride/archive/projects/PXD031716LiverProtein homogenates were prepared from either whole liver tissues or enriched mitochondria. For whole liver tissue, approximately 100 mg of tissue were isolated from snap frozen mouse livers that had been stored at -80˚C. Tissue was homogenized in 500 ul PBS supplemented with protease inhibitors (Thermo Fisher, 78425) and protein concentrations were quantitated (DC Protein assay, Biorad). 50 g of each sample was solubilized in 1% SDS, 50mM Tris pH 8.0 at a concentration of 5 mg/ml, run on a 4-20% Mini-PROTEAN TGX precast protein gel (BioRad) into the top of the resolving portion of the gel, stained with Coomassie Blue, and destained. For enriched mitochondria, approximately 100mg of liver tissue was homogenized with 40 strokes of a dounce homogenizer in mitochondrial isolation buffer [HEPES (5mM), sucrose (70mM), mannitol (220mM), MgCl2 (5mM), KH2PO4 (10mM), and EGTA (1mM), pH 7.2] supplemented with protease inhibitors (Thermo Fisher, 78425). Mitochondria were isolated via differential centrifugation: First nuclei and cell debris were removed by isolating supernatant after five spins at 600g; and enriched mitochondria were then pelleted with a 10000g spin. Protein concentrations were quantitated (DC protein assay, BioRad), and 50 g of each sample was solubilized in 1% SDS, 50mM Tris pH 8.0 at a concentration of 5 mg/ml. Samples were run on a 4-20% Mini-PROTEAN TGX precast protein gel (BioRad) into the top of the resolving portion of the gel, stained with Coomassie Blue, and destained. Samples were analyzed at the UT Southwestern Proteomics Core. Gel slices were cut into 1 mm3 cubes, and digested overnight with trypsin (Pierce 90058) following reduction and alkylation with DTT and iodoacetamide (Sigma–Aldrich I6125). The samples then underwent solid-phase extraction cleanup with an Oasis HLB plate (Waters) and the resulting samples were injected onto an Orbitrap Fusion Lumos mass spectrometer coupled to an Ultimate 3000 RSLC-Nano liquid chromatography system. Samples were injected onto a 75 m i.d., 75-cm long EasySpray column (Thermo) and eluted with a gradient from 0-28% buffer B over 90 min. Buffer A contained 2% (v/v) ACN and 0.1% formic acid in water, and buffer B contained 80% (v/v) ACN, 10% (v/v) trifluoroethanol, and 0.1% formic acid in water. The mass spectrometer operated in positive ion mode with a source voltage of 1.8 kV, ion transfer tube temperature of 275 °C, MS1 AGC target of 400000, MS1 maximum injection time of 50 ms, intensity threshold of 5000, MS2 AGC target of 10000, MS2 maximum injection time of 35 ms, MS2 isolation window of 1.6 Da. MS1 scans were acquired at 120,000 resolution in the Orbitrap and up to 10 MS/MS spectra were obtained in the ion trap for each full spectrum acquired using higher-energy collisional dissociation (HCD) for ions with charges 2-7. Dynamic exclusion was set for 25 s after an ion was selected for fragmentation.PrideNot availableRaw MS data files were analyzed using Proteome Discoverer v2.4 SP1 (Thermo), with peptide identification performed using Sequest HT searching against the mouse protein database from UniProt (downloaded January 2022; 17,062 sequences) assuming a trypsin digestion (cleavage after Lys and Arg except when immediately followed by Pro). Fragment and precursor tolerances of 10 ppm and 0.6 Da were specified, and three missed cleavages were allowed. Carbamidomethylation of Cys was set as a fixed modification, with oxidation of Met set as a variable modification. The false-discovery rate (FDR) cutoff was 1% for all peptides and all PSMs were validated with the Percoloator node within Proteome Discoverer. PSMs found in only a subset of samples were re-searched to identify peptides based on retention time and mass. Protein abundance was quantitated based on the total ion count for all identified peptides in each sample and only proteins notated with FDR <1% were considered. Protein abundances were normalized after log2 transform according to previously published protocols using Microsoft Excel 2019 (60): First, abundance values were log2 transformed, then each sample was normalized by the average abundance and data distribution width. Missing values were imputed as random values centered around 2.5 standard deviations below the mean abundance value. For samples with > 4 replicates, Shapiro-Wilk tests were performed to test for normal distributions. If samples were not normally distributed, Mann-Whitney tests were used to calculate p-values. For normally distributed data, F-tests were used to assess equal variance, followed by two-tailed homoscedastic (or heteroscedastic) tests to calculate p-values. Unsupervised hierartchical clustering was performed on z-score transformed values in MATLAB (Mathworks, Inc.). Mitochondrial-localized proteins were classified based on their presence in MitoCarta3.0 (61).ProteomicsPrashant MishraChildren's Research Institute University of Texas Southwestern Medical CenterPARTIALMus Musculus (mouse)Lesner NP, Wang X, Chen Z, Frank A, Menezes CJ, House S, Shelton SD, Lemoff A, McFadden DG, Wansapura J, DeBerardinis RJ, Mishra P. Differential requirements for mitochondrial electron transport chain components in the adult murine liver. Elife. 2022 11:e80919 10.7554/ELIFE.80919nicholas.lesner@pennmedicine.upenn.eduHuman proteome projectUniversity of PennsylvaniaUnited Statesdisorder of liver (disorder), Disease of liver, LIVER DIS, Disease, Liver and Intrahepatic Bile Duct Disorder, Liver, Liver Dysfunction, Liver disorder in pregnancy NOS (disorder), Proteins, Liver dysfunction, Hepatic Disorder, Gene, Unspecified disorder of liver, Dysfunctions, disease or disorder of liver, mitochondrial, liver disease or disorder, Liver disorder NOS, Liver disorder in pregnancy unspecified (disorder), liver disease, Liver Dysfunctions, Liver disorder in pregnancy, mild, ATP synthase D chain, unspecified as to episode of care., Protein, Diseases, Gene Products, LIVER DISORDER NOS, hepatic disorder, Dysfunction, liver disorder in pregnancy - delivered, liver and intrahepatic bile duct disorder, Liver disorder NOS (disorder), Liver Diseases, LD - Liver disease, hepatic disease, liver disorder antepartum, Mitochondrial, Liver disorder in pregnancy - delivered (disorder), Liver disorder in pregnancy (disorder), Disorder of liver (disorder), with delivery, [X]Diseases of the liver (disorder), disease of the liver (disorder), Liver Disorder, disorder of liver, disease of liver, Protein Gene Products, disease of liver [Ambiguous], Gene Proteins, Hepatopathy, [X]Diseases of the liver, Disorder of liver, liver disorderl(3)rK137, Bru, IPP2A2, 1110033A15Rik, E 920, Raw, Hgfr, ion, CRL2, AI326280, cleavage, DHFRP2, 5730555F13Rik, protein, PSBG-1, AI845540, PBG1, DNB1, CG17228, CER2, 1135/09, 5730420M11Rik, peptide, 1135/07, Polypeptides, protein polypeptide chains, caudal, peptido, HGFR, dorsal marginal zone, 0451/09, 2-amino-3-mercaptopropanoic acid, Cystein, ARG, Arg, protein aggregate, sty, WMS, ARP, Svc, average, C, SET, 0244/09, mKIAA0458, (S)-2-amino-5-guanidinopentanoic acid, CERL2, peptides, K, MUB3_18, TAF-I, cisteina, ATN1L, M, PS-beta-C|D, PSG95, (S)-2-Amino-5-guanidinovaleric acid, DROPROSA, R, epsilon-diaminocaproic acid, proteins, arg, MUB3.18, l(3)j6E2, EG:196F3.2, DmelCG4299, 671/2, IGAAD, set, 0320/10, DMPROSPER, LYS, Lys, DmelCG10574, CD66f, Lysin, ppm, sample, RG7MT1, GPHYSD2, Racemethionine, DANTE, Lzm, SGS, L-Arginin, phapii, c-SP1, Lzp, eye-lt-m03Jus-gt-, proto-oncogene c-Met, l(3)rO534, PRO, Pro, EG:171D11.4, 2-Amino-4-(methylthio)butyric acid, arginine, StF-IT-1, 9130215G10Rik, CYS, Cys, ACMICD, PROS-1, PROS-2, count, Ionen, ATP synthase D chain, pro, 1316/02, BcDNA:HL08040, Henson's node, Methionin, Lzm-s1, Hmet, CG14784, (2R)-2-amino-3-sulfanylpropanoic acid, and GLY protein 2, CG18104, CG14783, parent ion, HGF receptor, HGF/SF receptor, oligonucleotide random primer, Mitochondrial, and GLY protein 1, HLA-DR-associated protein II, Voila, DI-2, PSGIIA, I-2Dm, 0664/07, 1167/13, alpha, CG4299, MASS, AA450830, hCMT1c, c-Met, beta Trypsin, TAF[[II]], L-Cystein, I-2PP1, Lysm, Spn1, TAF-IBETA, CKTSF1B3, precursor ion, PS-beta-G-1, PSGGA, DMZ, l(1)16Fg, TAF-Ibeta, Polypeptide, DmelCG32810, 0989/01, i2pp2a, pds, mouse protein, 2-amino-4-(methylsulfanyl)butanoic acid, localised, number, L-Zystein, FBN, Gene, precursor, protein-containing complex, 0763/13, mitochondrial, PHAPII, L-(+)-arginine, l(3)10419, dmTAF8, l(3)rQ526, HGF, B1G1, PSBG1, DmelCG17228, (2S)-2-amino-5-(carbamimidamido)pentanoic acid, polypeptide chain, ECTOL1, HL-VIII, Gene Products, CG5896, anon-WO0140519.15, F15E12.6, Del(8)44H, Prosp, CG9456, RCCP2, l(3)j5E11, Sp1-1, DmelCG18104, PROS, F15E12_6, 0585/13, localized, Gm17754, scatter factor receptor, nodus primitivus, iones, E-920, ipp2a2, beta-Trypsin, CG7128, Argos, 2pp2a, CG4531, AW742570, Hcys, ions, CG10574, AUTS9, l(3)rH013, OCTD, prod, Prodos, RANDOM, 2PP2A, 2-amino-4-(methylthio)butanoic acid, 10^[-6], CYSTEINE, taf-ibeta, stem node, DmAos, dSET, dSet, L-Arg, FREE CYSTEINE, peptidos, SP2, SP1, sp1, AI838057, (2R)-2-amino-3-mercaptopropanoic acid, TAF, 0563/18, metionina, rlt, ABLL, 0671/02, l(3)j12C8, Lyzs, data, DmelCG9456, DmelCG5896, Grass, DL-Methionine, Atr2, protein complex, Proteins, igaad, tailed, Peptide, Abll, l(3)rJ806, group, presence., Pros, polypeptide, l(3)rJ812, l(3)rL433, count in organism, l(3)rI160, native protein, 0441/16, natural protein, I-2PP2A, Dm I-2, Protein, I2PP2A, TFIID, MFS1, 1110003E12Rik, l(3)05959, AI414665, Zystein, Data Base, MET, ATCMPG1, Met, WMS2, ATCMPG2, Random selection by shearing, Col4a-1, COCO, PRSS, ensemble, nodal stem, gil, 2-amino-3-sulfanylpropanoic acid, GREM3, tyrosine-protein kinase Met, (2S)-2-amino-5-guanidinopentanoic acid, Tripcellim, L-2-Amino-3-mercaptopropionic acid, eyes3, (R)-2-amino-3-mercaptopropanoic acid, l(3)05845, 2-Amino-3-mercaptopropionic acid, sample population, l(3)rK204, Protein Gene Products, FL-NCA-1|2, DmelCG4531, Trypure, Gene Proteins, dSET/TAF-Ibeta, Aos, 2610030F17Rik, DmelCG7128, Ion, l(3)rJ472, SSKS, alpha-amino-gamma-methylmercaptobutyric acid, AA536808, E920, Peptid, Par4, EG:65F1.3, TAF8, AA407739, 5730455C01Rik, CG32810, SF receptordisorder of liver (disorder), Peptidomics., Disease of liver, LIVER DIS, Disease, Liver and Intrahepatic Bile Duct Disorder, Liver, Liver Dysfunction, Liver disorder in pregnancy NOS (disorder), Liver dysfunction, Hepatic Disorder, Unspecified disorder of liver, Dysfunctions, disease or disorder of liver, mitochondrial, liver disease or disorder, Liver disorder NOS, Liver disorder in pregnancy unspecified (disorder), liver disease, Liver Dysfunctions, Liver disorder in pregnancy, mild, ATP synthase D chain, Diseases, LIVER DISORDER NOS, hepatic disorder, Dysfunction, liver disorder in pregnancy - delivered, liver and intrahepatic bile duct disorder, Liver disorder NOS (disorder), Liver Diseases, LD - Liver disease, hepatic disease, liver disorder antepartum, Mitochondrial, Liver disorder in pregnancy - delivered (disorder), Liver disorder in pregnancy (disorder), Disorder of liver (disorder), with delivery, [X]Diseases of the liver (disorder), disease of the liver (disorder), Liver Disorder, disorder of liver, disease of liver, disease of liver [Ambiguous], unspecified as to episode of care, Hepatopathy, [X]Diseases of the liver, Disorder of liver, liver disordersodium salt, Water, ammonium formate, dalpha-SNAP, 13C-labeled, Apoplexy, cadmium salt, mannite, ion, E-421, Laboratory, CVA (Cerebrovascular Accident), H(2)O, D-(-)-Mannitol, BOUND WATER, SDH, CREB3L1, magnesium formate, Mitochondrial Contractions, mg/ml, oxidane, House Mouse, zinc salt, heavy chain disease, Osmofundin, Long Term, WATER, l(2)k09905, HOH, l(1)AA33, 5730420M11Rik, dmTAF[[II]]230, SDS, protein polypeptide chains, Magnesium chloride, cobalt(II) formate dihydrate, Protease Inhibitor, 1, DGS, 2, 3, 4, 5, dJ1068F16.2, (R*, DmelCG8428, SET, TGX, Spin, C79325, TFIID TAF250, Transglutaminase X, cel, STROKE, Tissue, Salt, Processed cyclic AMP-responsive element-binding protein 3-like protein 1, proteins, T6G21.3, SNAP-25, Q3 spectrum, AI047805, DmelCG4299, isolation and purification, set, TGx, SPIN, Peptidohydrolase Inhibitor, column, l(1)B2/13.1, BTKAP1, anatomical tube, ACN, sample, Acn, CT, Acute Cerebrovascular Accidents, GTFII-I, nickel salt, 6030441I21Rik, 2-Trifluoroethanol, dTAF[[II]]230, l(1)LB9, Stars, (2R, BZRP, cobalt (+2) salt, mannitol, Ct, Liquid Chromatography, TAF200, Longterm Effect, BAP135, not genetically inherited, PBS, PBR, Brain Vascular Accident, Acute Cerebrovascular Accident, MgCl2, H2O, sodium (4:1:1) salt, ATP synthase D chain, Dm1, magnesium salt, Hydrolase Inhibitor, simple tissue, BAP-135, Hydrogen Oxide, E 421, Single-Stage Mass Spectrometry, CVAs (Cerebrovascular Accident), HLA-DR-associated protein II, Antagonists, DI-2, I-2Dm, alpha-D-Glucopyranoside, Swiss Mouse, formate, Shwachman-Diamond type metaphyseal dysplasia, cromium (+3), Shwachman syndrome, GtfII-I, beta Trypsin, CG1106, I-2PP1, Cerebrovascular accident (disorder), Painful bladder syndrome, l(2)W5, TAF-IBETA, Taf250, stroke, duct, Charges and Fees, RIC4, ammonium (4:1) salt, Mouse, TAF-Ibeta, Endopeptidase, Peptide Peptidohydrolase Inhibitor, lead (+2) salt, STARS, 4-Dimercapto-2, Cerebrovascular accident (disorder) [Ambiguous], TAF230, BPBS, Old astrocyte specifically-induced substance, Brain, F10B6_15, rac-Dithiothreitol, l(1)VE614, nickel formate dihydrate, lead formate, Peptidomics, Effects, Endopeptidase Inhibitors, threo-1, Cleland's reagent, aluminum salt, Pierce, beta-D-Fruf-(2<->1)-alpha-D-Glcp, Kern, TGase X, protein-containing complex, abdominal muscles, mitochondrial, 2310007C07Rik, 17alpha)-isomer, F10B6.15, CG7826, agua, jecur, isolation, IT, Oasis, Gene Products, Mitochondrion, CG7835, CG6625, Mus musculus domesticus, CG42273, Peptide Hydrolase, nucleo atomico, Stroke/CVA - undefined, iones., anatomical systems, dTAF[[II]]250, Stroke and cerebrovascular accident unspecified, Acinus, 2310061K05Rik, thallium (+1) salt, Longterm, cell, Contraction, Monosodium Salt, beta-Trypsin, rubidium salt, 4733401P19Rik, 2pp2a, pk18, Long-Term, (L)-Mannitol, methanoic acid, ions, Antagonist, kf, MBR, 2-7, CG10574, CD156, acinusL, Brain Vascular Accidents, Injectable, dTAF250, TG(X), 2PP2A, Protease Antagonists, acinusS, congenital lipomatosis of pancreas, dSET, dSet, PTBR, Peptide Peptidohydrolase Inhibitors, 4-(2-hydroxyethyl)-, LB5, 3-butanediol, WBSCR6, DmelCG6625, Magnesiumchlorid, Proteins, BG:DS00004.13, potassium formate, Cerebrovascular accident, copper (+2) salt, Rnt, buffer, Cell, Dithiothreitol, mKIAA0670, bnch, dTAF230, lLB5, l(2)k02511, native protein, sacarosa, Temperatures, I-2PP2A, cupric formate, Long Term Effects, Dm I-2, (CVA), TAF[[II]]250/230, 5alpha, eagle-Barrett syndrome, aluminum formate, dJ142L7.2, Magnesium chloride anhydrous, DTL, Taf[[II]]250, AU020952, DTT, lithium formate, ensemble, Stroke NOS, Striated muscle activator of Rho-dependent signaling, Chloride, OASIS, SWDS, House Mice, Shwachman-Bodian-Diamond syndrome, Cerebrovascular Accident, Peptidohydrolase Inhibitors, mDRC, CD156a, Longterm Effects, ME-IV, Gene Proteins, Peptidase, Acute, Magnesium chloride (MgCl2), Antiprotease, Pancreatic insufficiency and bone marrow dysfunction, Stroke and cerebrovascular accident unspecified (disorder), Fees, Peptide Hydrolase Inhibitors, [OH2], CVA (cerebral vascular accident), DIWS, IPP2A2, Endopeptidase Inhibitor, TGASEX, Strokes, SEC9, Mus domesticus, leg, Peptide Hydrolase Inhibitor, zinc formate, HEPES Monosodium Salt, lead salt, protein, Trifluoroethyl Alcohol, bA416N4.2, Atomkern, Alkylations, Acute Stroke, Cut, Ethanol, ammonium tetraformate, BANF, l(1)7Ba, congenital, l(1)7Bb, Q1 spectrum, CVA, Inhibitors, Vascular Accidents, Williams-Beuren syndrome chromosomal region 6 protein, Min, cerebral infarction, protein aggregate, Hydrolase Inhibitors, Injectables, Effect, SYNDROME, alpha-SNAP, DmelCG42273, HEPES Monosodium, 3-hydroxy-5-estrane-17-carbonitrile, nucleus atomi, TAF-I, long, 5R)-Hexane-1, 2610036I19Rik, 2610510L13Rik, E430039A18Rik, iecur, min, Swiss Mice, mAPC, IBP, purification, copper, manna sugar, CG11387, lipomatosis of pancreas, Stroke, IGAAD, dulcite, fSAP152, lithium salt, SOLO DANCERS, 1-Piperazineethanesulfonic acid, DmelCG10574, RUN, Run, TGASE5, DmelCG1106, Diws1t, dihydridooxygen, Shwachman-Bodian syndrome, 2.3.2.13, house mouse, Bladder pain syndrome, dihydrogen oxide, ammonium (2:1) salt, Long-Term Effects, 3R, phapii, Tube, TGase-5, Alcohol, Peptidase Inhibitor, 2-trifluoro-, [MgCl2], mouse, aqua, Dmel_CG7826, AI836084, StF-IT-1, 4-dimercapto-2, TAFII-250, Peptide Peptidohydrolase, Cerebrovascular Stroke, TAF250/230, Cerebrovascular Apoplexy, beta-D-fructofuranosyl, Osmitrol, AA33, 4R, 6-hexol, TUBE, TAFII250, HCD, Ionen, SRF-Phox1-interacting protein, Trifluoroethyl, BcDNA:GH10590, nickel (+2) salt, Cerebrovascular, Cerebrovascular Accidents, Dmel_CG7835, Mnb, MNB, Mus musculus, l(2R)W5, absence of, ammonium salt, Mitochondrial, Cerebral Stroke, Painful Bladder Syndrome, table sugar, CHARGES, 1-alpha-D-Glucopyranosyl-2-beta-D-fructofuranoside, MS/MS, mice, Xylella fastidiosa str. Temecula1, cobaltous formate, hydrogen hydroxide, alphaSnap, CG4299, AW124434, CG17603, TAF[[II]], acqua, domesticus, Cerebrovascular Strokes, R*)-1, Peptidase Inhibitors, Charge, WBS, SR3-5, Acetamide, copper salt, Antiproteases, CG8428, Wasser, HEPES, Inhibitor, i2pp2a, Transglutaminase-5, Bladder Pain Syndrome, CG1849, d230, noyau atomique, dalphaSNAP, full spectrum, cesium salt, Gene, Protease, dTAFII250, ACINUS, TGM6, EfW1, TFII-I, PHAPII, Buffer, Bruton tyrosine kinase-associated protein 135, formic acid, potassium salt, polypeptide chain, House, dmTAF1, Ms1, Taf230, Injection, 14C-labeled, l(3)77ABa, E421, Sacharose, IB291, l(3)77ABc, Protease Antagonist, RIC-4, AA673430, Mice, Cerebral, TAF250, nuclei, Taf200, Vascular Accident, Saccharose, MS1, Swiss, 6-hexaol, iones, MS2, Runt, nucleo, noyau, gel, D-Mannitol, ipp2a2, Taf1p, Mannitol, eau, calcium formate, Charges, Xylella fastidiosa (strain Temecula1 / ATCC 700964), Stroke NOS (disorder), Fee, DmelCG11387, nucleus, taf-ibeta, male sterility 1, N-2-Hydroxyethylpiperazine-N'-2'-ethanesulfonic Acid, cromium (+3) salt, Long-Term Effect, AA553322, BTK-associated protein 135, TAF, TGMX, 2-iodo-, sodium formate, DYRK1, TAF[[II]]250, dSNAP, nickel formate, DBI, protein complex, 5R)-hexane-1, DmelCG1849, igaad, strontium formate, l(3)84Ab, dsnap, strontium salt, Magnesium, Peptide, group, Dithiotreitol, Contractions, Cerebrovascular Apoplexya, PSS2, natural protein, PKBS, Mus, p230, water, Protein, DL-threo-1, AI551343, I2PP2A, core, TFIID, 25/11, Dyrk1, (3alpha, tandem MS, HLB, PSEC0238, with urinary tract Abnormality and cryptorchidism, TAF[[II]]230, PRSS, MALE STERILITY 1 PROTEIN, CVA - Cerebrovascular accident unspecified, CC1, Tripcellim, N 2 Hydroxyethylpiperazine N' 2' ethanesulfonic Acid, l(1)19Ea, TAF[II]250, CGI-97, sample population, Cerebral Strokes, chromic formate, Laboratory Mice, Protein Gene Products, Mitochondrial Contraction, l(2)10403, P235, cAMP-responsive element-binding protein 3-like protein 1, Livers, Trypure, dSET/TAF-Ibeta, Acute Strokes, 2610030F17Rik, mitochondria, DmelCG17603, Cane sugar, Ion, concentration, SNAP, calcium salt, SCH, AA407739, Laboratory Mouse, White sugar, CEREBROVASCULAR ACCIDENT, RCB1376, snap, TAF1, 3-OHECNfalseMitochondrial Hepatopathy ProteomicsIdentification of altered proteins following mitochondrial hepatopathy2022-09-282022-09-05PXD031716NEWT:3555NEWT:71647NEWT:330879NEWT:35554NEWT:32046NEWT:544496NEWT:2042546NEWT:32049NEWT:1392696NEWT:259447NEWT:295546NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:5722NCBITaxon:1280NEWT:1129NEWT:309807NEWT:10245NEWT:55153NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:193516NEWT:43186NEWT:428146NEWT:498257NEWT:10036NEWT:1590NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:1147161NEWT:661410NEWT:638632NEWT:263737NEWT:224326NEWT:1333499NCBITaxon:79857NEWT:1096976NEWT:5702NEWT:95648NEWT:1589NEWT:135622NEWT:1349NEWT:35786NEWT:1580NEWT:399784NEWT:96731NEWT:383379NEWT:1401257NEWT:418106NEWT:10029NEWT:913645NEWT:44491NEWT:641809NEWT:27933NEWT:556484NEWT:317447NEWT:4688NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:3112NEWT:79329NEWT:4442NEWT:192875NEWT:1214915NEWT:12637NEWT:59729NEWT:2164133NEWT:295105NEWT:108061NEWT:60711NEWT:1316931NEWT:224308NEWT:3347NEWT:511145NEWT:160621NEWT:212790NEWT:931281NEWT:4432NEWT:5762NEWT:5763NEWT:658457NEWT:1310161NEWT:77133NEWT:295358NEWT:145481NCBITaxon:79824NEWT:2246NEWT:2652724NEWT:5757NCBITaxon:4563NEWT:5755NEWT:44689NEWT:44447NEWT:752555NEWT:498211NEWT:5759NEWT:1736231NEWT:5518NEWT:398007NEWT:1392NEWT:498217NEWT:498216NEWT:2242NEWT:11320NEWT:286NEWT:391619NEWT:246196NEWT:287NEWT:246197NEWT:145458NEWT:633149NEWT:1149786NEWT:10239NEWT:68895NEWT:44685NEWT:161934NEWT:4897NEWT:1148NEWT:5744NEWT:5508NEWT:3329NEWT:5507NEWT:410661NEWT:55571NEWT:35500NEWT:1235816NEWT:1140NCBITaxon:2157NEWT:1143NEWT:4896NEWT:1287689NEWT:1390NEWT:11557NEWT:1094343NEWT:1462472NEWT:1336795NEWT:644042NEWT:294NEWT:1773NEWT:1182590NEWT:3712NEWT:82380NEWT:3711NEWT:935293NEWT:375146NEWT:2065263NEWT:1772NEWT:118698NEWT:1616117NEWT:263NEWT:118696NEWT:52283NEWT:11988NEWT:284812NEWT:8175NEWT:400667NEWT:43330NEWT:1603293NEWT:408169NEWT:44664NEWT:47946NEWT:459758NEWT:3702NEWT:1245466NEWT:243277NEWT:1246791NEWT:7067NEWT:118694NEWT:2850NEWT:118691NEWT:33548NEWT:274NEWT:7070NCBITaxon:287NEWT:408172NEWT:408170NEWT:96794NEWT:3708NEWT:332648NEWT:44670NEWT:536231NEWT:376219NEWT:219813NEWT:1510NEWT:1436733NEWT:460519NEWT:1247411NEWT:1515NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:9483NEWT:333760NEWT:56636NEWT:1513458NEWT:1233681NCBITaxon:40559NEWT:506599NEWT:2853422NEWT:81077NEWT:84588NEWT:1679718NEWT:480NEWT:65349NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:300852NEWT:1502NEWT:128017NEWT:376686NEWT:95486NEWT:508771NEWT:1883446NEWT:253NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:93062NCBITaxon:4932NEWT:109760NEWT:943274NEWT:644223NEWT:235443NEWT:108458NEWT:5936NEWT:272623NEWT:272624NEWT:320637NEWT:886559NEWT:55521NEWT:983964NEWT:118499NEWT:11706NEWT:32644NEWT:527796NEWT:1130798NEWT:55529NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:476272NEWT:3747NEWT:195051NEWT:3988NEWT:1116234NEWT:1255228NEWT:649908NEWT:410289NEWT:373153NEWT:3983NEWT:352472NEWT:1071661NEWT:1202532NEWT:360094NEWT:470NEWT:41364NEWT:1313NEWT:1218097NCBITaxon:50557NEWT:39251NEWT:39491NCBITaxon:5811NEWT:29491NEWT:101841NEWT:2014887NEWT:33952NEWT:153009NEWT:261756NEWT:571256NEWT:40483NEWT:63366NEWT:63367NEWT:688333NEWT:215402NCBITaxon:548681NEWT:318586NCBITaxon:5820NEWT:272634NEWT:198107NEWT:9031NEWT:1872122NEWT:1308NEWT:7091NEWT:1034015NEWT:760568NEWT:141262NEWT:108931NEWT:1055524NEWT:4087NEWT:9778NEWT:306NEWT:150475NEWT:303NEWT:267872NEWT:97477NEWT:7111NEWT:347515NEWT:172269NEWT:9534NEWT:6269NEWT:5180NEWT:256737NEWT:4090NEWT:4092NEWT:9541NEWT:8694NEWT:4093NEWT:4096NEWT:8692NEWT:2903NEWT:185579NEWT:941442NEWT:536088NEWT:13076NEWT:1226408NEWT:7108NEWT:40479NEWT:43989NEWT:4076NEWT:142615NEWT:317NEWT:1006581NEWT:885318NEWT:39488NEWT:550NEWT:4081NEWT:273068NEWT:554NEWT:98334NEWT:451516NEWT:4084NEWT:63577NEWT:36185NEWT:1225786NEWT:7574NEWT:1715256NEWT:30640NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:114155NEWT:6494NEWT:6491NEWT:507601NEWT:520NEWT:186441NEWT:643680NEWT:214092NCBITaxon:6157NEWT:6239NEWT:162425NEWT:216257NEWT:102169NEWT:208893NEWT:9986NEWT:4054NEWT:8654NEWT:8658NEWT:1268063NEWT:8655NEWT:5147NEWT:98351NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:28104NEWT:207559NEWT:407821NCBITaxon:2NEWT:85057NEWT:985076NEWT:568708NEWT:1215323NEWT:986NEWT:52641NEWT:7159NEWT:267671NEWT:28532NEWT:353152NEWT:8496NEWT:7165NEWT:27448NEWT:40674NEWT:1194669NEWT:51329NEWT:243230NEWT:443906NEWT:1080772NEWT:519NEWT:8479NEWT:8485NEWT:6063NEWT:49240NEWT:1328388NEWT:104395NEWT:9557NEWT:6289NEWT:377586NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:9554NEWT:118562NEWT:725NEWT:7137NEWT:469008NEWT:256318NEWT:9321NEWT:206411NCBITaxon:6191NEWT:596153NEWT:229533NEWT:1836NEWT:214053NEWT:80863NEWT:90675NEWT:105231NEWT:104385NEWT:74649NEWT:52638NEWT:57075NEWT:206672NEWT:4097NEWT:8697NEWT:8695NEWT:884204NEWT:9785NEWT:6279NEWT:1123869NEWT:9544NEWT:9545NEWT:7370NEWT:9793NEWT:83906NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:1134506NEWT:255470NEWT:27457NEWT:38783NEWT:4006NEWT:6426NCBITaxon:5476NEWT:113208NEWT:6669NEWT:9935NEWT:89453NEWT:287889NEWT:1114301NEWT:51515NEWT:588596NEWT:51511NEWT:8845NEWT:92867NEWT:92866NEWT:419610NEWT:5334NEWT:51750NEWT:202950NEWT:382352NEWT:413071NCBITaxon:10239NCBITaxon:714NEWT:295027NCBITaxon:11320NEWT:632957NEWT:11051NEWT:9925NEWT:89462NCBITaxon:9606NEWT:452680NEWT:8839NEWT:90690NEWT:1436183NEWT:4232NEWT:2758385NEWT:416348NEWT:11298NEWT:171101NEWT:714NEWT:5322NEWT:421932NEWT:312017NEWT:196627NEWT:200308NEWT:553470NEWT:200302NEWT:870435NEWT:284593NEWT:100392NEWT:655819NEWT:5315NEWT:9913NEWT:265872NEWT:105841NEWT:337330NEWT:2666255NEWT:2285NEWT:999810NCBITaxon:5693NEWT:28995NEWT:1392998NEWT:380394NEWT:981087NEWT:180066NEWT:226900NEWT:1266738NEWT:231490NEWT:244704NEWT:7725NEWT:430615NEWT:563041NEWT:72664NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1416333NEWT:35024NEWT:135461NEWT:1678078NEWT:7962NEWT:749906NEWT:418985NEWT:1817808NEWT:749907NEWT:145262NEWT:145263NEWT:69014NEWT:142809NEWT:694569NEWT:27606NEWT:9739NEWT:59202NEWT:9975NEWT:8643NEWT:51953NEWT:3197NEWT:860688NEWT:13443NEWT:8644NEWT:36329NEWT:62615NEWT:5141NEWT:46360NCBITaxon:3044782NEWT:1333550NEWT:160235NEWT:72407NEWT:8639NEWT:1928434NEWT:8637NEWT:349741NEWT:409859NEWT:157295NEWT:2096NEWT:509169NEWT:192222NEWT:9721NEWT:137221NEWT:9963NEWT:1617910NEWT:565050NEWT:409860NEWT:2785785NEWT:411901NEWT:8626NEWT:105884NCBITaxon:9615NEWT:8621NEWT:58334NEWT:452659NEWT:1193501NEWT:58331NEWT:6689NEWT:8624NEWT:5116NEWT:8623NEWT:5597NCBITaxon:3077NEWT:298315NEWT:8618NEWT:70448NEWT:9704NEWT:698936NEWT:1009874NEWT:8855NEWT:9940NEWT:3168NEWT:1247190NEWT:521001NEWT:192011NEWT:1813887NEWT:118702NEWT:118700NEWT:2126719NEWT:224911NEWT:118705NCBITaxon:11552NEWT:334249NEWT:6945NEWT:184922NEWT:6703NEWT:2104NEWT:6943NEWT:2NEWT:157546NEWT:870726NEWT:474186NEWT:38942NEWT:1493NEWT:307972NEWT:1496NEWT:2102NEWT:3674NEWT:660469NEWT:1365186NEWT:38949NEWT:45474NEWT:1299114NEWT:1590841NEWT:2282334NEWT:4513NEWT:1247NEWT:70667NEWT:869728NEWT:5843NEWT:6938NCBITaxon:39054NCBITaxon:10407NEWT:1736309NEWT:4751NEWT:10360NEWT:47664NEWT:317548NEWT:15819NEWT:3654NEWT:237561NEWT:1085956NEWT:5833NEWT:3659NEWT:6928NEWT:1239NEWT:36745NEWT:499229NEWT:31298NEWT:400727NEWT:272563NEWT:11009NEWT:82688NEWT:5823NEWT:3885NEWT:3888NEWT:5821NEWT:44139NEWT:649408NCBITaxon:4895NCBITaxon:4896NEWT:315393NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:28909NEWT:515849NEWT:3641NEWT:466585NEWT:1000589NEWT:85963NEWT:85962NEWT:42197NEWT:143361NEWT:12993NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:1299NEWT:316407NEWT:34305NCBITaxon:183674NEWT:167387NEWT:84645NEWT:44586NEWT:1123294NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:431241NEWT:4550NEWT:1000561NEWT:2133NEWT:4555NEWT:294381NEWT:197NEWT:1390363NEWT:35889NEWT:288705NEWT:29176NEWT:4787NEWT:3217NEWT:4547NEWT:3218NEWT:556182NEWT:1270NEWT:374990NEWT:1432059NEWT:154293NEWT:156471NEWT:4784NEWT:3694NEWT:360106NEWT:156476NEWT:4543NEWT:4785NEWT:360104NEWT:1857017NEWT:10117NEWT:10359NEWT:10116NEWT:1280NEWT:10358NEWT:1735272NEWT:83334NEWT:83332NEWT:317513NEWT:580240NEWT:294128NEWT:11676NEWT:100226NEWT:718308NEWT:4530NEWT:176299NEWT:54126NEWT:75058NEWT:13616NEWT:296543NEWT:173NEWT:316435NEWT:42157NEWT:1895NEWT:1034304NEWT:105023NEWT:866628NEWT:64152NEWT:4924NEWT:749200NEWT:2748NEWT:8049NEWT:9378NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:203124NEWT:192952NEWT:230741NEWT:8296NEWT:117008NCBITaxon:1313NEWT:167748NEWT:44544NEWT:529818NEWT:400540NEWT:4911NEWT:645463NEWT:152480NEWT:129249NEWT:1077286NEWT:943129NEWT:1221594NEWT:990119NEWT:77216NEWT:1111708NEWT:227321NEWT:493760NEWT:281677NEWT:106590NEWT:260710NEWT:257313NEWT:3827NEWT:400772NEWT:128161NEWT:930945NEWT:1162296NEWT:106592NEWT:46704NEWT:482428NEWT:1187947NEWT:269796NEWT:10312NCBITaxon:1773NEWT:54571NEWT:9598NEWT:8030NEWT:1423772NEWT:53232NEWT:1639NEWT:188229NEWT:246437NEWT:3818NEWT:4909NEWT:759272NEWT:432359NEWT:1182263NEWT:943146NEWT:2711NEWT:537457NEWT:479249NEWT:9103NEWT:715877NEWT:862238NEWT:29159NEWT:10306NEWT:9595NEWT:8022NEWT:145943NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:29158NEWT:3633NEWT:3635NEWT:5811NEWT:3879NEWT:180923NEWT:1328881NEWT:411483NEWT:76336NEWT:3870NEWT:884019NEWT:198215NEWT:411490NEWT:200298NEWT:169963NEWT:587NEWT:588NEWT:585NEWT:343NEWT:225117NEWT:746128NEWT:5801NEWT:43665NEWT:212035NCBITaxon:1653394NEWT:1266464NEWT:1205NEWT:367830NEWT:178616NEWT:375451NEWT:357NEWT:9172NEWT:2770979NEWT:2010198NEWT:1277351NEWT:457428NEWT:106178NEWT:411469NEWT:84023NEWT:1679NEWT:559292NEWT:569NEWT:411464NEWT:411460NEWT:2762NEWT:1174673NEWT:1328426NEWT:562NEWT:28035NEWT:411470NEWT:2094720NCBITaxon:2697049NCBITaxon:138NEWT:28038NEWT:1663NEWT:62141NEWT:1423NEWT:4932NEWT:3603NEWT:2756NEWT:2759NEWT:3848NEWT:3847NEWT:38293NEWT:1428NEWT:339NEWT:327159NEWT:178876NEWT:1660NEWT:2751NEWT:327160NEWT:573NEWT:571NEWT:12922NEWT:241368NEWT:518766NEWT:42528NEWT:2664373NEWT:190802NEWT:9417NEWT:1216979NEWT:7237NEWT:509924NEWT:115104NEWT:1121114NEWT:663NEWT:8570NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:220668NEWT:1821314NEWT:1249668NEWT:426430NEWT:9407NEWT:9646NEWT:5286NEWT:7227NEWT:7469NEWT:9402NEWT:964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