Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/anti-KRT17_antibody_PeptideGroups.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/IgG_PSMs.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/anti-KRT17_antibody_Proteins.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/parameters.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/IgG_PeptideGroups.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/anti-KRT17_antibody_MSMSSpectrumInfo.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/anti-KRT17_antibody_PSMs.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/ResultStatistics.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/IgG_MSMSSpectrumInfo.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/IgG_Proteins.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/anti-KRT17_antibody.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036433/IgG.rawprimaryOK200liangwf7@mail2.sysu.edu.cnWenfeng liangMass SpectrometryGel-based experimentColon CancerNot availableMouse colon cancer mshttps://www.ebi.ac.uk/pride/archive/projects/PXD036433Gastrointestinal System EpitheliumColonThe gel was chopped into small fragments with a razor blade, destained, and subjected to digestion by modified porcine trypsin (50–100 ng per digestion; Promega).Pridentact peptides were detected in the Orbitrap at a resolution of 70 000. Peptides were selected for MS/MS using NCE setting as 27; ion fragments were detected in the Orbitrap at a resolution of 17 500. A data-dependent procedure that alternated between one MS scan followed by 20 MS/MS scans was applied for the top 20 precursor ions above a threshold ion count of 1E4 in the MS survey scan with 30.0s dynamic exclusion.ProteomicsGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor DiseasesThe Sixth Affiliated Hospital, Sun Yat-sen UniversityPARTIALMus Musculus (mouse)liangwf7@mail2.sysu.edu.cnNot availableHuman proteome projectBiology/disease-driven human proteome project (b/d-hpp)Antibodies (b/d-hpp)The Sixth Affiliated Hospital, Sun Yat‑Sen UniversityChinasmall, Trypure, PRSS, reduced, underdeveloped, DmelCG1106, Pro-Mega., gel, DGS, beta-Trypsin, hypoplasia, Tripcellim, tiny, beta Trypsin, CG1106measuring, colon-38 cell., MC38 cell, determination, colon 38 cell, Proteins, Gene, colon38 cancer cell, B-cell receptor complex, Spectrum Analyses, antibodies, MC38-adenocarcinoma cell, Spectrum Analysis, PC2, Spectroscopy, antibody, BCR complex, MS, chemical analysis, Protein, K17, Mass, Gene Products, Analysis, immunoglobulin complex, Mass Spectrum Analyses, Mass Spectroscopy, Ka17, opsonin activity, Mass Spectrum Analysis, Mass Spectrum, PCHC1, Analyses, membrane bound, Spectrometry, colon38 cell, Protein Gene Products, Gene Proteins, immunoglobulin, B cell receptor accessory molecule complex, mouse colon 38- adenocarcinoma cell, scientific observation, B lymphocyte receptor complex, B cell receptor activity, colon-38 cancer cell, PC, assay, B-lymphocyte receptor complex, Krt1-17Intervention, parent ion, Intervention Strategies, peptides, ion, Procedures, interventionDescription, MS/MS, MS2, iones, Interventional, Methodological, precursor, Procedure, ions, Methodological Study, SCAN, Peptide, SURGICAL AND MEDICAL PROCEDURES, Intervention or Procedure, SCAN., Study, Polypeptides, Techniques, peptido, Methodological Studies, precursor ion, count, Ionen, Ion, Method, Studies, Peptid, peptidos, Polypeptide, Technique, tandem MSopsonin activity, IgG Antibody, PCHC1, IgG, Immunoglobulin GT, IgG(T), Immunoglobulin, Peptidomics, membrane bound, Polyglobin, B-cell receptor complex, colorectal carcinoma cell, antibodies, PC2, antibody, immunoglobulin, Antibody, BCR complex, B cell receptor accessory molecule complex, IgG3, CRC cell., IgG4, IgG1, Gamma Globulin, IgG2, IgG2A, IgG2B, GT, B lymphocyte receptor complex, 7S Gamma Globulin, K17, B cell receptor activity, Allerglobuline, PC, B-lymphocyte receptor complex, Krt1-17, immunoglobulin complex, 7S, Ka17falseTo elucidate the molecular mechanism behind Krt17-mediated regulation of immune system process, we conducted a co-IP assay using the anti-KRT17 antibody coupled with mass spectrometry (MS) analysis to identify the KRT17-interacting proteins in MC38 cellProteomics analysi of bound to anti-KRT17 antibody compared to IgG in colorectal cancer cell.2023-03-092022-08-31PXD036433NEWT:6945NEWT:3555NEWT:2NEWT:157546NEWT:35554NEWT:38942NEWT:307972NEWT:32046NEWT:544496NEWT:2042546NEWT:45351NEWT:43179NEWT:4513NEWT:5722NEWT:376741NEWT:55153NCBITaxon:10407NEWT:1736309NEWT:309800NEWT:1211601NEWT:876138NEWT:3654NEWT:237561NEWT:5833NEWT:6928NEWT:10036NEWT:36745NEWT:1351NEWT:1438992NEWT:2649997NEWT:272563NEWT:224326NCBITaxon:79857NEWT:1096976NEWT:95648NEWT:3885NEWT:3888NEWT:1589NEWT:135622NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:3880NEWT:272559NEWT:3641NEWT:383379NEWT:466585NEWT:10029NEWT:913645NEWT:1000589NEWT:85963NEWT:85962NEWT:317447NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:192875NEWT:34305NEWT:59729NCBITaxon:183674NEWT:224308NEWT:84645NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:1283NEWT:931281NEWT:4550NEWT:1000561NEWT:197NEWT:1390363NCBITaxon:79824NEWT:4787NCBITaxon:4563NEWT:5755NEWT:44689NEWT:3218NEWT:5759NEWT:1736231NEWT:1270NEWT:2242NEWT:4784NEWT:11320NEWT:360106NEWT:286NEWT:287NEWT:10117NEWT:10239NEWT:10116NEWT:1280NEWT:1735272NEWT:83334NEWT:83332NEWT:44685NEWT:317513NEWT:1148NEWT:580240NEWT:294128NEWT:11676NEWT:55571NEWT:100226NEWT:4530NEWT:4896NEWT:75058NEWT:13616NEWT:1390NEWT:1094343NEWT:296543NEWT:1773NEWT:1895NEWT:1182590NEWT:3712NEWT:935293NEWT:64152NEWT:4924NEWT:749200NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:263NEWT:230741NEWT:52283NEWT:284812NCBITaxon:1313NEWT:43330NEWT:1603293NEWT:408169NEWT:44544NEWT:4911NEWT:645463NEWT:3702NEWT:129249NEWT:243277NEWT:990119NEWT:408172NEWT:408170NEWT:493760NEWT:260710NEWT:257313NEWT:400772NEWT:3708NEWT:128161NEWT:332648NEWT:106592NEWT:536231NEWT:460519NEWT:1187947NEWT:1432138NEWT:10312NEWT:1424507NCBITaxon:1773NEWT:9598NEWT:8030NEWT:1639NEWT:188229NEWT:3818NEWT:480NEWT:4909NEWT:67767NEWT:432359NEWT:46835NEWT:1182263NEWT:2711NEWT:376686NEWT:95486NEWT:9103NEWT:29159NEWT:253NEWT:10306NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:8022NEWT:145943NCBITaxon:4932NEWT:595536NEWT:240906NEWT:593117NEWT:3635NEWT:5811NEWT:235443NEWT:272624NEWT:411483NEWT:884019NEWT:198215NEWT:411490NEWT:983964NEWT:169963NEWT:32644NEWT:225117NEWT:499175NEWT:109779NEWT:476272NEWT:3747NEWT:195051NEWT:367830NEWT:1255228NEWT:178616NEWT:410289NEWT:373153NEWT:352472NEWT:357NEWT:360094NEWT:470NEWT:1313NEWT:411469NEWT:84023NEWT:559292NEWT:39491NCBITaxon:5811NEWT:411464NEWT:411460NEWT:2014887NEWT:2762NEWT:1174673NEWT:562NEWT:411470NEWT:33952NEWT:2094720NCBITaxon:2697049NEWT:571256NEWT:28038NEWT:1663NEWT:1423NEWT:4932NEWT:3603NEWT:2759NEWT:3847NEWT:327159NEWT:178876NEWT:327160NEWT:573NEWT:9031NEWT:7091NEWT:108931NEWT:241368NEWT:42528NEWT:190802NEWT:9778NEWT:150475NEWT:303NEWT:9417NEWT:7111NEWT:347515NEWT:1216979NEWT:5180NEWT:256737NEWT:115104NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:185579NEWT:941442NEWT:220668NEWT:13076NEWT:1249668NEWT:7108NEWT:317NEWT:7227NEWT:7469NEWT:885318NEWT:9402NEWT:415540NEWT:550NEWT:675060NEWT:4081NEWT:334542NEWT:554NEWT:98334NEWT:426428NEWT:7574NEWT:1715256NEWT:7215NEWT:575412NEWT:29204NEWT:2172103NEWT:507601NEWT:643680NCBITaxon:6157NEWT:746360NEWT:6239NEWT:470150NEWT:216257NEWT:102169NEWT:9986NEWT:4054NEWT:73239NEWT:226186NEWT:1268063NEWT:8782NEWT:1263854NEWT:435590NEWT:1902NEWT:160488NEWT:28104NEWT:1908NEWT:13164NCBITaxon:2NEWT:985076NEWT:1215323NEWT:52641NEWT:7038NEWT:6192NEWT:28532NCBITaxon:38727NEWT:353152NEWT:2829NEWT:366581NEWT:216599NEWT:216595NEWT:51329NEWT:243230NEWT:8355NEWT:9685NEWT:7029NEWT:1080772NEWT:8479NEWT:1283300NEWT:6183NEWT:6063NEWT:630NEWT:334747NEWT:61235NEWT:15368NEWT:6289NEWT:436486NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:725NEWT:170187NEWT:260707NCBITaxon:6191NEWT:1836NEWT:185431NEWT:29760NEWT:260704NEWT:703612NEWT:260705NEWT:80863NEWT:2697049NEWT:105231NEWT:1216981NEWT:4097NCBITaxon:6073NEWT:884204NEWT:6279NEWT:1123869NEWT:9544NEWT:7370NEWT:83906NEWT:607699NEWT:6282NEWT:208964NEWT:1134506NEWT:575584NEWT:38783NEWT:8727NEWT:4006NEWT:8726NEWT:6426NEWT:6669NEWT:10090NEWT:4120NEWT:51515NEWT:5693NEWT:8724NEWT:51511NEWT:92867NEWT:8723NEWT:5334NEWT:37334NEWT:382352NCBITaxon:10359NEWT:242619NEWT:632957NEWT:34073NEWT:373995NEWT:5689NEWT:544404NEWT:9925NEWT:8839NEWT:4232NEWT:2758385NEWT:37349NEWT:4113NEWT:837NEWT:11298NEWT:171101NEWT:714NEWT:36015NEWT:421932NEWT:196627NEWT:5691NEWT:48479NEWT:627025NEWT:1097677NEWT:61674NEWT:1117957NEWT:9913NEWT:8709NEWT:4100NEWT:1076NEWT:6763NEWT:803NEWT:29722NEWT:380394NEWT:1692259NEWT:180066NEWT:135588NEWT:1843183NEWT:58002NEWT:326423NEWT:36111NEWT:4577NEWT:1416333NEWT:5664NEWT:2157NEWT:1678078NEWT:749906NEWT:418985NEWT:146479NEWT:1911079NEWT:69373NEWT:264203NEWT:1480154NEWT:1274414NEWT:27606NEWT:59202NEWT:9975NEWT:9612NEWT:38865NEWT:51953NEWT:3197NEWT:9615NEWT:10299NEWT:860688NEWT:112273NEWT:36329NEWT:1147787NCBITaxon:3044782NEWT:1440772NEWT:72407NEWT:1355477NEWT:349741NEWT:9605NEWT:9606NEWT:90371NEWT:157295NEWT:641501NEWT:7668NEWT:915099NEWT:74940NEWT:9721NEWT:137221NEWT:9600NEWT:1450511NEWT:1143193NEWT:411901NEWT:9838NEWT:105884NCBITaxon:9615NEWT:9839NEWT:58334NEWT:1193501NEWT:3055NEWT:58331NEWT:6326NEWT:6689NEWT:5476NEWT:299767NEWT:1274432NEWT:1274426NEWT:70448NEWT:9825NEWT:1274423NEWT:393765NEWT:698936NEWT:39946NEWT:9823NEWT:9940NEWT:95602NEWT:521001NEWT:39947NEWT:1274420NEWT:5784580000-0002-4664-8772