Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051703/mqpar.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051703/P14657.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051703/P14655.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051703/P14667.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051703/P14663.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051703/P14661.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051703/P14659.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051703/P14665.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051703/txt.zipprimaryOK200tuula.nyman@medisin.uio.noTuula NymanData-dependent acquisitionMass SpectrometryBottom-up proteomicsAerobic adaptationSurfaceome analysisPropionibacterium freudenreichiiVitamin b12https://www.ebi.ac.uk/pride/archive/projects/PXD051703Preparing the cell samples for proteomics and enzymatic cell-surface shaving. The surfaceome samples were harvested from mid-log (I) growth phases of both aerobic and anaerobic fermentations by centrifugation (4 min, 4 °C, 3320× g). The cells were washed with 0.1 M sodium acetate (pH 5.0, Sigma-Aldrich) and resuspended into 50 mM TEAB (17% sucrose) (triethylammonium bicarbonate buffer, Sigma-Aldrich) after centrifugation (4 min, 4 °C, 3320× g). Enzymatic surface shaving was carried out with 0.05 µg/µL of trypsin (Promega, Madison, WI, USA) for 30 min at 37 °C. Afterward, the protein-cell suspensions were first time centrifuged (4 min, RT, 4000× g) to ensure cell-free supernatant and second time centrifuged (2 min, RT, 16 000× g) through Spin-X® filters to recover the peptides and the enzyme. Flow-troughs were left to incubate for 17 h at 37 °C before the tryptic digestions were terminated with the addition of trifluoroacetic acid (TFA, Sigma-Aldrich) at a final concentration of 0.6%. The peptide concentrations were measured spectrophotometrically with NanoDrop- 1000 (Thermo Fisher Scientific, DE, USA). Samples were stored at - 20 °C. LC-MS/MS identification. The tryptic peptides were concentrated and purified using ZipTips C18 (Merck Millipore) according to the manufacturer’s instructions. An equal amount of each peptide sample was loaded into an Easy-nLC 1000 nano-LC system (Thermo Scientific, Waltham, MA, USA) coupled with a quadrupole Orbitrap mass spectrometer (Q Exactive Plus, ThermoElectron, Bremen, Germany) equipped with a nano-electrospray ion source (EASY- Spray Thermo Scientific, Waltham, MA, USA), and analyzed as previously described by Savijoki et al (1). Ref (1): Savijoki K, Miettinen I, Nyman TA, Kortesoja M, Hanski L, Varmanen P, Fallarero A. 2020. Growth Mode and Physiological State of Cells Prior to Biofilm Formation Affect Immune Evasion and Persistence of Staphylococcus aureus. Microorganisms. 2020 Jan 12;8(1):106. doi: 10.3390/microorganisms8010106.PrideNot availableThe acquired MS raw files, for protein identification and label-free quantification (LFQ), were searched against an in-house database of PFR subsPFR DSM 20271T (CP010341) (18) with MaxQuant (ver. 1.6.1.0) under the following settings: carbamidomethyl (C) was specified as a fixed modification; oxidation of methionine was set as a variable modification; a peptide tolerance was set at 20 ppm in the first search and the main search error of 4.5 ppm was applied. Additionally, trypsin without proline restriction enzyme option was used, allowing two miscleavages; the false discovery rate (FDR) filtering was set to 1 % for both peptide and protein identification, and the minimal number of unique and razor peptides were defined as 1.ProteomicsTuula NymanHead of ProteomicsPARTIALPropionibacterium Freudenreichii Subsp. Freudenreichiituula.nyman@medisin.uio.noNot availableUniversity of OsloNorwayBacterium acidi propionici d, Gene Expression Monitoring, Profiling, Bacterium acidi propionici a, Transcriptome, Transcriptome Profiling, Analyses, mRNA, determination, Transcriptome Profilings, Monitorings, chemical analysis., Gene, Transcriptomics, Differential Displays, Transcript Expression, Transcriptome Analysis, Transcriptome Analyses, Monitoring, Gene Expression Profilings, Gene Expression, Differential Display, Transcript Expression Analysis, Gene Expression Analysis, mRNA Differential Displays, Transcript Expression Analyses, Gene Expression Analyses, Gene Expression Pattern Analysis, Gene Expression Monitorings, Propionicibacterium freudenreichii, Expression Analysis, assay, Analysis, Profilings, Expression Analyses, mRNA Differential DisplayBru, IPP2A2, 2-amino-4-(methylsulfanyl)butanoic acid, anon-WO0172774.86, Raw, anon-WO0172774.87, (-)-(S)-proline, L-alpha-pyrrolidinecarboxylic acid, DmelCG14121, L Isomer, Gene, anon-WO0172774.89, LFQ, protein, protein-containing complex, Prolin, PFR, PHAPII, 5730420M11Rik, peptide, Polypeptides, protein polypeptide chains, L-Isomer Methionine, Methionine, peptido, polypeptide chain, pyrrolidine-2-carboxylic acid, L-Proline, Gene Products, L-Methionine, Del(8)44H, protein aggregate, Pedameth, Svc, SET, Hpro, peptides, TAF-I, M, ipp2a2, P, beta-Trypsin, 2-Pyrrolidinecarboxylic acid, 2pp2a, proteins, (2S)-pyrrolidine-2-carboxylic acid, CG10574, PROLINE, DmelCG4299, (S)-2-pyrrolidinecarboxylic acid, IGAAD, set, 2-amino-4-(methylthio)butanoic acid, 2PP2A, 10^[-6], DmelCG10574, taf-ibeta, ppm, L Proline, dSET, dSet, peptidos, (S)-2-carboxypyrrolidine, metionina, Racemethionine, CG14121, phapii, l(3)S147910, DL-Methionine, protein complex, prolina, DL-Proline, Proteins, 2-Amino-4-(methylthio)butyric acid, igaad, StF-IT-1, L-Isomer, not genetically inherited, Peptide, group, polypeptide, native protein, natural protein, I-2PP2A, Protein, Dm I-2, I2PP2A, proline, Methionin, Hmet, Data Base, Met, 1479/10, (-)-2-pyrrolidinecarboxylic acid, Col4a-1, PRSS, HLA-DR-associated protein II, ensemble, DI-2, L-Prolin, prolinum, I-2Dm, Tripcellim, CG4299, (-)-proline, (S)-pyrrolidine-2-carboxylic acid, beta Trypsin, Protein Gene Products, I-2PP1, Gene Proteins, Trypure, dSET/TAF-Ibeta, 2610030F17Rik, TAF-IBETA, alpha-amino-gamma-methylmercaptobutyric acid, Peptid, TAF-Ibeta, Polypeptide, L-(-)-proline, AA407739, Liquimeth, i2pp2a, L-pyrrolidine-2-carboxylic acid, Peptide.Integral Membrane Proteins, Metabolic Networks, Networks, Materials, postnatal development., Esters, determination, postnatal development, Surface Proteins, ethanoate, (SP-4-2)-, growth and development, Membrane-Associated Proteins, Integral, Propanoate, 13, Partial Pressures, Background, Readability, primary metabolites, BTBD34, oxigeno, vitamin, Cultural, symptoms, Impacts, 26Fe, Environmental Impacts, E948, 8, Microeconomic, IKKg, multicellular organismal biosynthetic process, KEY, Key, imprinted and ancient gene protein, single-organism biosynthetic process, ethnicity, Theory, Utility Theories, reference sample, Household Consumption, ERV-R envelope protein, anabolism, O, 2-hydroxypropanoate metabolism, Surface, Oxygen-16, Economic Policies, Acetic Acid, hypoplasia, Pathways, alpha-hydroxypropionate metabolic process, N24)-, Living Costs, CH3-COO(-), Environmental Impact, Oxygen, SU, [OO], Derivatives, Ferrate(2-), Household Consumptions, Surface Protein, Membrane Associated Proteins, Economic, Theories, TM, Membrane Protein, B61, Envelope polyprotein, HERV-T Env protein, screening, Pathway, Home Economics, Propionate, Indices, Index, Eritron, Hypothesis, Krebs cycle, ACETATE ION, Aptitudes, Partial, Acetic Acid Esters, Metabolic Network, HERV-T_19q13.11 provirus ancestral Env polyprotein, Macroeconomic Factors, Ability, Economics, Transmembrane protein, B 12, 2-hydroxypropanoate metabolic process, DmIKKgamma, Economic Condition, Cultural Background, Protoheme, Household, Iron 56, Cultures, Sauerstoff, Utility Theory, dIKK, Kenny, Genetic Materials, Macroeconomic, Ethanoat, Genetic Material, Cell Membrane Protein, haeme, Disauerstoff, dioxygen, Vitamin, Factors, Cobalamins, Cultural Beliefs, growth pattern, Iron, non-developmental growth, Consumer Price Indices, vitamines, Membrane-Associated, IKK-gamma, 17-tetramethyl-21H, signs, Cell Membrane Proteins, ERV3 envelope protein, ERV-3 envelope protein, Home, Fe, Condition, EDP1, HERV-R_7q21.2 provirus ancestral Env polyprotein, Eisen, DmelCG16910, Easterlin Hypothesis, Heme b, Material, metabolites, Cistron, Acids, lactate metabolism, Propionic Acids, iron, E430016J11Rik, vitaminum, Propanoic, Remittances, Dioxygen, Integral Membrane, Policies, vitamine, Vitamin B12, N23, (7, N22, hem, Utility, secondary metabolites, E-948, Gene, 8O, biosynthesis, Network, vitamina, Ximpact, PFR, dIKK-gamma, hemos, Propanoic Acid, Easterlin, dioxygene, reduced, Azetat, Membrane-Associated Protein, DmIKK-gamma, citric acid cycle, dmIKKgamma, tiny, IKK[[gamma]], Backgrounds, Bacterium acidi propionici d, Cell Membrane, ERV3-1 envelope protein, Bacterium acidi propionici a, hierro, Genetic, formation, 18-dipropanoato(4-)-N21, haem, vitaminas, Microeconomic Factors, Cultural Relativisms, Propionic Acid, Economic Conditions, synthesis, Abilities, IKK, Capital, Acetic Acids, HERV-R envelope protein, Customs, vitamins, liquid, culture, Propionic Acid Derivatives, molecular oxygen, Controlled, Policy, 12-diethenyl-3, small, Cobalamin, Economic Policy, Controlling, Oxygen 16, findings, Conditions, Proteins, CI-B12, fer, ion(1-), Cell Surface, Cell Surface Protein, 23H-porphine-2, function, Factor, Production, Cultural Backgrounds, Living Cost, Surface protein, Cistrons, oxygen, Cell, impact-a, Impact, Consumption, Environmental, development, IKKgamma, Haem, Consumer Price Index, Consumer Price, oxidative TCA cycle, Acetate, Pressure, Cyanocobalamin, Propanoic Acids, chemical analysis, Protein, Membrane Proteins, IMPACT, imprinted and ancient gene protein homolog, oxygene, Propanoates, alpha-hydroxypropionate metabolism, Propanoic Acid Derivatives, OXYGEN MOLECULE, Cell Surface Proteins, Metabolic Pathways, Membrane Associated Protein, O2, ferrum, Acetic, dihydrogen, underdeveloped, Metabolic, Dmikkgamma, postnatal growth, metabolite, Talents, Iron-56, Understanding, Membrane, CG16910, hBACURD2, B12, TCA cycle, Integral Membrane Protein, Pressures, Remittance, Propionic, Biomasses, Talent, Cost of Living, MeCO2 anion, Environments, Relativisms, Metabolic Pathway, Propionicibacterium freudenreichii, assay, acetic acid, Relativism, E 948, Cultural Relativism, growth, Protoheme IX, RWDD5, Ferroprotoporphyrinliquid chromatography tandem mass spectroscopy, DIWS, TFA, postnatal development, passive evasion of immune response of other organism involved in symbiotic interaction, growth and development, jan, protein, Long Term, Mid(H15), l(2)k09905, dmTAF[[II]]230, Trifluoroacetate, peptide, Polypeptides, protein polypeptide chains, ref2p, peptido, B1, Min, Williams-Beuren syndrome chromosomal region 6 protein, protein aggregate, Effect, Staphlococcus pyogenes citreus, DmelCG42273, integumentum commune, ALY|REF, DmelCG8428, Spin, LCMSMS, TFIID TAF250, peptides, cel, Staphilococcus aureus, Moods, min, mAPC, proteins, Micrococcus aureus, Y, AI047805, BEF, SPIN, Immune, BTKAP1, Diws1t, trifluoro-, D1, sample, TF, REF, s, TEAB, Long-Term Effects, GTFII-I, jan A, 6030441I21Rik, LC-MS2, dTAF[[II]]230, external covering of organism, DmelCG10360, LC-MS/MS, TAF200, Dmel_CG7826, Longterm Effect, ref, Evasion, organism surface, TAFII-250, TAF250/230, BAP135, Fermentations, beta-D-fructofuranosyl, Thermo Scientific, doi., Ref2P, TAFII250, ref(2)Po2, mitigation of host immune response by virus, Dm1, BcDNA:AT12574, SRF-Phox1-interacting protein, passive evasion of host immune response, immune evasion, BAP-135, Dmel_CG7835, Acetic acid, H15r/nmr2, Mnb, MNB, l(2R)W5, Acid, table sugar, growth pattern, 1-alpha-D-Glucopyranosyl-2-beta-D-fructofuranoside, non-developmental growth, alpha-D-Glucopyranoside, Trifluoroacetic, AW124434, GtfII-I, CG17603, TAF[[II]], beta Trypsin, l(2)W5, Staphylococcus pyogenes aureus, Taf250, WBS, Cesium, integumentary system, SR3-5, liquid chromatography-tandem mass spectroscopy, Biocatalyst, CG8428, Polypeptide, janus, dermal system, TAF230, Sucrose, d230, Peptidomics, Effects, Biocatalysts, Ref(2)P, beta-D-Fruf-(2<->1)-alpha-D-Glcp, Gene, dTAFII250, Sodium Acetate Trihydrate, protein-containing complex, EfW1, TFII-I, CG7826, LC-MS-MS, DOI, Buffer, Bruton tyrosine kinase-associated protein 135, surface, Immune Evasions, polypeptide chain, dmTAF1, Taf230, Ref(2)p, p62, THOC4, passive immune evasion, LC-MSMS, CG7835, Gene Products, CG42273, Sacharose, IB291, Mood, AA673430, Pro-Mega, TAF250, Cesium Trifluoroacetate, Taf200, anatomical systems, dTAF[[II]]250, Saccharose, Longterm, cell, ALY/REF, los, beta-Trypsin, Taf1p, Sodium Acetate, Long-Term, dTAF250, Enzyme, DmelCG6634, Suspension, CG7933, Evasions, peptidos, Long-Term Effect, AA553322, BTK-associated protein 135, Staphylococus aureus, SUCROSE, TAF, Anhydrous, active evasion of host immune response, PTHB1, ref(2)Pn, WBSCR6, DYRK1, TAF[[II]]250, protein complex, ALY, Affects, Proteins, l(3)84Ab, ref(2)p, BG:DS00004.13, buffer, Cell, Peptide, bnch, dTAF230, CD142, development, l(2)k02511, polypeptide, LC/MS/MS, mitigation by virus of host immune response, H15r, sacarosa, native protein, natural protein, C18, p230, sucrose, Protein, Long Term Effects, TAF[[II]]250/230, TFIID, Dyrk1, 25/11, Taf[[II]]250, AU020952, DmelCG7933, TAF[[II]]230, PRSS, Staphylococcus aureus subsp. anaerobius, suppression by virus of host immune response, body surface, nmr2, CC1, postnatal growth, Tripcellim, CG6634, TAF[II]250, TBX20, extra, beta-D-fructofuranosyl alpha-D-glucopyranoside, sample population, Longterm Effects, ME-IV, Streptococcus aureus, Protein Gene Products, l(2)10403, evasion by virus of host immune response, Trypure, Gene Proteins, mitigation of host immune response, DmelCG17603, Micrococcus pyogenes, Cane sugar, concentration, liquid chromatography tandem mass spectrometry, Peptid, Jan, CG10360, growth, White sugar, TAF1falseAerobic Adaptation and Metabolic Dynamics of Propionibacterium freudenreichii DSM 20271: Insights from Comparative Transcriptomics and Surfaceome AnalysisPropionibacterium freudenreichii (PFR) DSM 20271 is a bacterium known for its ability to thrive in diverse environments and produce vitamin B12. Despite its anaerobic preference, recent studies have elucidated its ability to prosper in the presence of oxygen, prompting a deeper exploration of its physiology under aerobic conditions. Here, we investigated the response of DSM 20271 to aerobic growth by employing comparative transcriptomic and surfaceome analyses alongside metabolite profiling. Cultivation under controlled partial pressure of oxygen (pO2) conditions revealed significant increases in biomass formation and altered metabolite production, notably B12 vitamin, pseudovitamin-B12, propionate and acetate, under aerobic conditions. Transcriptomic analysis identified differential expression of genes involved in lactate metabolism, TCA cycle, and electron transport chain, suggesting metabolic adjustments to aerobic environments. Moreover, surfaceome analysis unveiled growth environment-dependent changes in surface protein abundance, with implications for sensing and adaptation to atmospheric conditions. Supplementation experiments with key compounds highlighted the potential for enhancing aerobic growth, emphasizing the importance of iron and α-ketoglutarate availability. Furthermore, in liquid culture, FeSO4 supplementation led to increased heme production and reduced vitamin B12 production, highlighting the impact of oxygen and iron availability on the metabolic pathways. These findings deepen our understanding of PFR's physiological responses to oxygen availability and offer insights for optimizing its growth in industrial applications.2024-09-092024-04-24PXD051703NEWT:3555NEWT:71647NEWT:35554NEWT:32046NEWT:544496NEWT:2042546NEWT:32049NEWT:1392696NEWT:259447NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:5722NCBITaxon:1280NEWT:1129NEWT:55153NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:10036NEWT:1590NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:1147161NEWT:224326NEWT:1333499NCBITaxon:79857NEWT:1096976NEWT:95648NEWT:1589NEWT:135622NEWT:96731NEWT:383379NEWT:10029NEWT:913645NEWT:44491NEWT:556484NEWT:317447NEWT:4688NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:4442NEWT:192875NEWT:59729NEWT:2164133NEWT:108061NEWT:1316931NEWT:224308NEWT:3347NEWT:511145NEWT:931281NEWT:658457NEWT:77133NEWT:295358NEWT:145481NCBITaxon:79824NCBITaxon:4563NEWT:5755NEWT:44689NEWT:44447NEWT:5759NEWT:1736231NEWT:1392NEWT:498217NEWT:2242NEWT:11320NEWT:286NEWT:391619NEWT:246196NEWT:287NEWT:246197NEWT:633149NEWT:10239NEWT:44685NEWT:161934NEWT:1148NEWT:5508NEWT:5507NEWT:410661NEWT:55571NEWT:35500NEWT:1140NEWT:1143NEWT:4896NEWT:1390NEWT:11557NEWT:1094343NEWT:1336795NEWT:644042NEWT:1773NEWT:1182590NEWT:3712NEWT:82380NEWT:935293NEWT:375146NEWT:118698NEWT:263NEWT:118696NEWT:52283NEWT:284812NEWT:8175NEWT:43330NEWT:1603293NEWT:408169NEWT:44664NEWT:47946NEWT:3702NEWT:243277NEWT:7067NEWT:118694NEWT:2850NEWT:118691NEWT:33548NEWT:274NEWT:408172NEWT:408170NEWT:3708NEWT:332648NEWT:44670NEWT:536231NEWT:376219NEWT:1436733NEWT:460519NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:9483NEWT:1513458NCBITaxon:40559NEWT:84588NEWT:1679718NEWT:480NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:300852NEWT:1502NEWT:376686NEWT:95486NEWT:508771NEWT:1883446NEWT:253NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:93062NCBITaxon:4932NEWT:644223NEWT:235443NEWT:108458NEWT:272623NEWT:272624NEWT:320637NEWT:983964NEWT:118499NEWT:32644NEWT:527796NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:476272NEWT:3747NEWT:195051NEWT:3988NEWT:1255228NEWT:649908NEWT:410289NEWT:373153NEWT:352472NEWT:1071661NEWT:360094NEWT:470NEWT:41364NEWT:1313NEWT:39491NCBITaxon:5811NEWT:29491NEWT:2014887NEWT:33952NEWT:261756NEWT:571256NEWT:40483NEWT:272634NEWT:9031NEWT:1872122NEWT:7091NEWT:141262NEWT:108931NEWT:1055524NEWT:4087NEWT:9778NEWT:306NEWT:150475NEWT:303NEWT:267872NEWT:7111NEWT:347515NEWT:9534NEWT:5180NEWT:256737NEWT:4090NEWT:4092NEWT:9541NEWT:4093NEWT:4096NEWT:185579NEWT:941442NEWT:13076NEWT:7108NEWT:40479NEWT:4076NEWT:317NEWT:1006581NEWT:885318NEWT:39488NEWT:550NEWT:4081NEWT:273068NEWT:554NEWT:98334NEWT:451516NEWT:36185NEWT:1225786NEWT:7574NEWT:1715256NEWT:30640NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:6494NEWT:6491NEWT:507601NEWT:643680NEWT:214092NCBITaxon:6157NEWT:6239NEWT:162425NEWT:216257NEWT:102169NEWT:9986NEWT:4054NEWT:1268063NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:28104NEWT:407821NCBITaxon:2NEWT:985076NEWT:1215323NEWT:986NEWT:52641NEWT:7159NEWT:28532NEWT:353152NEWT:8496NEWT:27448NEWT:1194669NEWT:51329NEWT:243230NEWT:1080772NEWT:519NEWT:8479NEWT:8485NEWT:6063NEWT:49240NEWT:104395NEWT:6289NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:725NEWT:469008NEWT:256318NEWT:9321NEWT:206411NCBITaxon:6191NEWT:596153NEWT:229533NEWT:1836NEWT:80863NEWT:105231NEWT:52638NEWT:57075NEWT:4097NEWT:884204NEWT:9785NEWT:6279NEWT:1123869NEWT:9544NEWT:7370NEWT:83906NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:1134506NEWT:38783NEWT:4006NEWT:6426NCBITaxon:5476NEWT:6669NEWT:9935NEWT:89453NEWT:51515NEWT:588596NEWT:51511NEWT:8845NEWT:92867NEWT:92866NEWT:5334NEWT:51750NEWT:202950NEWT:382352NEWT:413071NEWT:632957NEWT:9925NEWT:89462NEWT:8839NEWT:90690NEWT:4232NEWT:2758385NEWT:11298NEWT:171101NEWT:714NEWT:421932NEWT:312017NEWT:196627NEWT:200308NEWT:200302NEWT:870435NEWT:284593NEWT:100392NEWT:5315NEWT:9913NEWT:265872NEWT:105841NEWT:337330NEWT:2666255NEWT:2285NEWT:999810NCBITaxon:5693NEWT:1392998NEWT:380394NEWT:981087NEWT:180066NEWT:226900NEWT:430615NEWT:563041NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1416333NEWT:1678078NEWT:749906NEWT:418985NEWT:749907NEWT:145263NEWT:694569NEWT:27606NEWT:9739NEWT:59202NEWT:9975NEWT:51953NEWT:3197NEWT:860688NEWT:13443NEWT:36329NEWT:62615NCBITaxon:3044782NEWT:160235NEWT:72407NEWT:349741NEWT:157295NEWT:2096NEWT:192222NEWT:9721NEWT:137221NEWT:9963NEWT:1617910NEWT:565050NEWT:2785785NEWT:411901NEWT:105884NCBITaxon:9615NEWT:58334NEWT:1193501NEWT:58331NEWT:6689NEWT:298315NEWT:70448NEWT:698936NEWT:9940NEWT:521001NEWT:192011NEWT:1813887NEWT:118702NEWT:118700NEWT:224911NEWT:118705NCBITaxon:11552NEWT:6945NEWT:184922NEWT:6703NEWT:2104NEWT:2NEWT:157546NEWT:474186NEWT:38942NEWT:307972NEWT:1496NEWT:2102NEWT:45474NEWT:4513NEWT:1247NEWT:6938NEWT:376741NCBITaxon:10407NEWT:1736309NEWT:4751NEWT:10360NEWT:47664NEWT:317548NEWT:15819NEWT:3654NEWT:237561NEWT:5833NEWT:6928NEWT:1239NEWT:36745NEWT:272563NEWT:82688NEWT:3885NEWT:3888NEWT:5821NEWT:44139NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:28909NEWT:515849NEWT:3641NEWT:466585NEWT:1000589NEWT:85963NEWT:85962NEWT:143361NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:1299NEWT:34305NCBITaxo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