Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD051822/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD051822/InGel-ctrl-2-zb-20201214.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD051822/InGel-CD-825-ZB-20201001.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD051822/InGel-CD-2-zb-20201214.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD051822/InGel-ctr-825-ZB-20201001.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD051822/InGel-ctrl-2-zb-20201209.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD051822/InGel-CD-2-zb-20201209.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD051822/Search.zipprimaryOK200bozheng@njmu.edu.cnYueshuai GuoMass SpectrometryShotgun proteomicsRna pulldownLc-ms/msLncrnaCdiptosphttps://www.ebi.ac.uk/pride/archive/projects/PXD051822CDIPTOSP was transcribed in vitro using T7 RNA polymerase and labeled with biotin. The biotinylated RNA was then tethered to streptavidin magnetic beads. Next, the RNA-bound beads were incubated with cellular lysates for 2 hours at 4°C. Magnetic beads were then pelleted and washed three times with 1X wash buffer. The bound protein was eluted by heating the beads at 95 °C for 5 min with 2× SDS loading buffer and then separated by SDS-PAGE. The gel particles were cut into blocks approximately 1 mm3 in size for in-gel digestion. Gel particles were first washed with deionized water, 50% acetonitrile (ACN), and 100% ACN. Next, the proteins were reduced and alkylated for 45 min at room temperature in the dark. The gel particles were then thoroughly washed, dried naturally, and subjected to protein digestion at 37 °C for 12 h. Trifluoroacetic acid (TFA), at a final concentration of 0.1%, was added to stop the digestion, and the supernatants were transferred to fresh tubes. All extracts and supernatants were combined and dried in a SpeedVac. The digested peptides were desalted with StageTip and analyzed using an LTQ Orbitrap Velos mass spectrometer.PrideNot availableThe MaxQuant software (version 1.6.5.0) was used for searching the raw files against UniProt human proteome database. The FDR of identified peptides and proteins was set to 1%. Searches were performed using Trypsin/P enzyme specificity while allowing up to two missed cleavages. Carbamidomethylation of cysteine residues (+57.0215Da) were set as fixed modifications. Variable modifications included oxidation of methionines and acetylation of protein N termini.ProteomicsBo ZhengThe Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital,Suzhou 215002, ChinaPARTIALHomo Sapiens (human)gys_706@163.com42325760 Tong R, Yu X, Xu B, Wu T, Liu M, Shen C, Xu X, Xia F. LncRNA CDIPTOSP-induced destabilization of KLF17 promotes ovarian cancer progression through STAU1-mediated mRNA decay. Am J Transl Res. 2026 18(5):4189-4203 10.62347/nzis8956Nanjing Medical UniversityChinaOvarian cancer (OC) remains one of the most lethal gynecologic malignancies, largely due to its poorly understood pathogenesis, which limits the development of effective early detection and targeted therapy. This study was designed to explore the potential role of the long non-coding RNA CDIPTOSP in OC progression. We observed high expression of CDIPTOSP in OC tissues and cell lines. Knockdown of CDIPTOSP impeded the proliferation and migration of OC cells. Using the RNA pull-down-Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) approach, we found that CDIPTOSP bound staufen double-stranded RNA binding protein 1 (STAU1). In turn, CDIPTOSP-STAU1 interactions were essential for KLF transcription factor 17 (KLF17) mRNA destabilization. Notably, depletion of KLF17 could rescue the tumor-suppressive effects caused by CDIPTOSP knockdown, whereas overexpression of KLF17 abolished the tumor-promoting effects induced by overexpressing CDIPTOSP. In conclusion, our study provided the first evidence of the CDIPTOSP/STAU1/KLF17 axis in the regulation of OC progression.LncRNA CDIPTOSP-induced destabilization of KLF17 promotes ovarian cancer progression through STAU1-mediated mRNA decay.Tong Ruiqing R, Yu Xiangling X, Xu Bingya B, Wu Tiantian T, Liu Meng M, Shen Cong C, Xu Xiangxiang X, Xia Fei FfalseLncRNA CDIPTOSP RNA pulldown-LC-MS/MSTo identify proteins that interact with CDIPTOSP in human ovarian cancer SKOV3 cells, RNA pulldown experiments were performed and subjected to three independently LC-MS/MS analysis and Label-free quantification.2026-06-292024-04-28PXD051822NEWT:6945NEWT:184922NEWT:6703NEWT:3555NEWT:71647NEWT:2NEWT:157546NEWT:35554NEWT:38942NEWT:307972NEWT:32046NEWT:544496NEWT:2102NEWT:2042546NEWT:259447NEWT:45351NEWT:43179NEWT:4513NEWT:180454NEWT:5722NEWT:1247NEWT:1129NEWT:376741NEWT:55153NCBITaxon:10407NEWT:1736309NEWT:309800NEWT:281395NEWT:10360NEWT:1211601NEWT:876138NEWT:47664NEWT:317548NEWT:3654NEWT:237561NEWT:5833NEWT:6928NEWT:10036NEWT:36745NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:272563NEWT:224326NCBITaxon:79857NEWT:1096976NEWT:82688NEWT:95648NEWT:3885NEWT:3888NEWT:5821NEWT:1589NEWT:135622NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:28909NEWT:515849NEWT:3641NEWT:383379NEWT:466585NEWT:10029NEWT:913645NEWT:1000589NEWT:85963NEWT:85962NEWT:143361NEWT:317447NEWT:4688NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:398580NEWT:4442NEWT:4565NEWT:1264690NEWT:515619NEWT:192875NEWT:34305NEWT:59729NCBITaxon:183674NEWT:224308NEWT:84645NEWT:44586NEWT:626528NEWT:3347NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:931281NEWT:4550NEWT:1000561NEWT:294381NEWT:197NEWT:1390363NEWT:77133NEWT:288705NEWT:29176NEWT:145481NCBITaxon:79824NEWT:4787NCBITaxon:4563NEWT:5755NEWT:44689NEWT:3218NEWT:5759NEWT:1736231NEWT:556182NEWT:1270NEWT:374990NEWT:1392NEWT:498217NEWT:156471NEWT:2242NEWT:4784NEWT:11320NEWT:360106NEWT:156476NEWT:286NEWT:391619NEWT:360104NEWT:246196NEWT:287NEWT:246197NEWT:10117NEWT:10239NEWT:10116NEWT:1280NEWT:1735272NEWT:83334NEWT:83332NEWT:44685NEWT:317513NEWT:1148NEWT:580240NEWT:5508NEWT:294128NEWT:11676NEWT:55571NEWT:35500NEWT:1140NEWT:100226NEWT:4530NEWT:1143NEWT:4896NEWT:75058NEWT:13616NEWT:1390NEWT:1094343NEWT:1336795NEWT:296543NEWT:316435NEWT:42157NEWT:1773NEWT:1895NEWT:1182590NEWT:3712NEWT:82380NEWT:105023NEWT:866628NEWT:935293NEWT:64152NEWT:4924NEWT:749200NEWT:375146NEWT:9378NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:263NEWT:230741NEWT:52283NEWT:284812NEWT:8175NCBITaxon:1313NEWT:43330NEWT:1603293NEWT:408169NEWT:44544NEWT:47946NEWT:4911NEWT:645463NEWT:3702NEWT:129249NEWT:1077286NEWT:243277NEWT:990119NEWT:2850NEWT:408172NEWT:227321NEWT:408170NEWT:493760NEWT:106590NEWT:260710NEWT:257313NEWT:3827NEWT:400772NEWT:3708NEWT:128161NEWT:332648NEWT:106592NEWT:536231NEWT:46704NEWT:1436733NEWT:460519NEWT:1187947NEWT:572307NEWT:1432138NEWT:269796NEWT:10312NEWT:1424507NCBITaxon:1773NEWT:54571NEWT:9598NEWT:8030NEWT:9483NCBITaxon:40559NEWT:1639NEWT:188229NEWT:3818NEWT:480NEWT:4909NEWT:67767NEWT:759272NEWT:432359NEWT:46835NEWT:1182263NEWT:109757NEWT:943146NEWT:2711NEWT:300852NEWT:1502NEWT:376686NEWT:95486NEWT:9103NEWT:508771NEWT:1883446NEWT:29159NEWT:253NEWT:10306NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:8022NEWT:145943NCBITaxon:4932NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:29158NEWT:3635NEWT:5811NEWT:235443NEWT:180923NEWT:108458NEWT:272623NEWT:272624NEWT:411483NEWT:884019NEWT:198215NEWT:411490NEWT:983964NEWT:118499NEWT:169963NEWT:32644NEWT:527796NEWT:225117NEWT:746128NEWT:499175NEWT:109779NEWT:43665NEWT:1266464NEWT:476272NEWT:3747NEWT:195051NEWT:367830NEWT:1255228NEWT:178616NEWT:649908NEWT:410289NEWT:373153NEWT:375451NEWT:352472NEWT:357NEWT:1071661NEWT:360094NEWT:470NEWT:41364NEWT:1313NEWT:411469NEWT:84023NEWT:559292NEWT:39491NCBITaxon:5811NEWT:29491NEWT:411464NEWT:411460NEWT:2014887NEWT:2762NEWT:1174673NEWT:1328426NEWT:562NEWT:411470NEWT:33952NEWT:2094720NCBITaxon:2697049NEWT:571256NEWT:40483NEWT:28038NEWT:1663NEWT:1423NEWT:4932NEWT:3603NEWT:2759NEWT:3847NEWT:38293NEWT:1428NEWT:327159NEWT:178876NEWT:1660NEWT:327160NEWT:573NEWT:9031NEWT:1872122NEWT:7091NEWT:108931NEWT:241368NEWT:1055524NEWT:42528NEWT:190802NEWT:9778NEWT:150475NEWT:303NEWT:9417NEWT:7111NEWT:347515NEWT:1216979NEWT:7237NEWT:9534NEWT:5180NEWT:256737NEWT:9541NEWT:115104NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:185579NEWT:941442NEWT:220668NEWT:13076NEWT:1821314NEWT:1249668NEWT:7108NEWT:317NEWT:5286NEWT:7227NEWT:7469NEWT:885318NEWT:9402NEWT:9644NEWT:415540NEWT:550NEWT:675060NEWT:4081NEWT:334542NEWT:554NEWT:27592NEWT:98334NEWT:426428NEWT:451516NEWT:63459NEWT:1276815NEWT:36185NEWT:588858NEWT:9639NCBITaxon:11071NEWT:242231NEWT:7574NEWT:1715256NEWT:197221NEWT:7215NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:2172103NEWT:6494NEWT:7460NEWT:6491NEWT:507601NEWT:643680NCBITaxon:6157NEWT:42752NEWT:746360NEWT:6239NEWT:470150NEWT:162425NEWT:216257NEWT:102169NEWT:543734NEWT:9986NEWT:4054NEWT:73239NEWT:226186NEWT:1268063NEWT:8782NEWT:1263854NEWT:118503NEWT:2059687NEWT:115357NEWT:435590NEWT:1902NEWT:160488NEWT:28104NEWT:1908NEWT:13164NEWT:216129NCBITaxon:2NEWT:985076NEWT:1215323NEWT:2664179NEWT:986NEWT:52641NEWT:7038NEWT:7159NEWT:6192NEWT:28532NCBITaxon:38727NEWT:353152NEWT:2829NEWT:366581NEWT:216599NEWT:216595NEWT:1194669NEWT:51329NEWT:40559NEWT:243230NEWT:8355NEWT:633NEWT:9685NEWT:7029NEWT:1080772NEWT:8479NEWT:1283300NEWT:186990NEWT:632NEWT:6183NEWT:6063NEWT:630NEWT:49240NEWT:334747NEWT:61235NEWT:15368NEWT:6289NEWT:436486NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:725NE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