Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/pPyp-BPSites_20221109_QE_609_22NO09_AhnSM.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/proteinGroups_20230221_QE_630_23FE22_AhnSM.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/PhosphoSTYHSites_20221228_QE_617_22DE28_AhnSM.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/Me-BP_3_20230221_QE_630_23FE22_AhnSM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/Me-BP_1_20230221_QE_630_23FE22_AhnSM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/Supplementary4_20221228_QE_617_22DE28_AhnSM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/Me-BP_2_20230221_QE_630_23FE22_AhnSM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/Supplementary3_20221109_QE_609_22NO09_AhnSM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/pPyp-BP_2_20230221_QE_630_23FE22_AhnSM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/pPyp-BP_3_20230221_QE_630_23FE22_AhnSM.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053006/pPyp-BP_1_20230221_QE_630_23FE22_AhnSM.rawprimaryOK200jmkee@unist.ac.krSeungmin AhnMass SpectrometryBottom-up proteomicsQexactiveE.coliLc-ms/msCell lysatehttps://www.ebi.ac.uk/pride/archive/projects/PXD053006Cell CultureThe protein pellet was re-dissolved in 200 μL of denaturation buffer (1.6% SDS in DPBS) and quantified using a Pierce BCA protein assay kit (Thermo Scientific). 650 μg of the lysate was then diluted 8-fold (final SDS concentration of 0.2%) with DPBS. This mixture was loaded onto pre-equilibrated Pierce streptavidin agarose beads (100 μL, Thermo Scientific) and incubated for 2 hours on an end-over-end rotator. Afterward, the beads were washed sequentially with 200 μL of 0.2% SDS in DPBS, followed by 2 times with 200 μL DPBS and 2 times with 200 μL DI water. The beads were resuspended in 200 μL of the trypsin digestion buffer (1 M urea, 50 mM Tris, 1 mM CaCl2, pH 8.0), and 5 μg of trypsin/Lys-C Mix (Promega) was added. It was incubated overnight using a thermomixer (37 oC, 400 rpm). The digested solution was collected, and the beads were washed with an additional 100 μL water. The solution was acidified by adding formic acid (final concentration 0.4%). The solution was desalted using a homemade C18 stage-tipliv and dried in a vacuum concentrator for subsequent LC-MS/MS analyses. All proteomic samples were analyzed with a Q Exactive Plus Orbitrap mass spectrometer (Thermo Scientific) in combination with a nanoelectrospray ion source (EASY-Spray). Following drying, samples were resuspended in an aqueous solution of 2% acetonitrile and 0.05 % TFA and subsequently loaded to the LC-MS/MS. Digested peptides were separated using a C18 column (PepMap RSLC C18, 2 μm, 100 Å, 75 μm * 50 cm) with a gradient of acetonitrile (from 4% to 32.5% over 120 minutes) in aqueous 0.1% formic acid at a flow rate of 300 nL/min. MS2 analysis involved precursor ion scanning of MS spectra (m/z 400 ~ 2000) in the Orbitrap at a resolution of 70,000 at m/z 400, with an internal lock mass. The top 15 ions were isolated and fragmented by high-energy collision-induced dissociation (HCD). All the experiments were carried out in triplicates. For individual protein analysis, 400 ng to 1 μg of trypsin-digested proteins were analyzed. For digestion, Pierce Trypsin Protease (MS grade, Thermo Scientific) was used following the in-solution digestion protocol from the manufacturer with a minor modification. For peptide separations, an LC gradient of acetonitrile (from 4% to 23% over 50 minutes) in aqueous 0.1% formic acid was used. Other conditions were identical to the proteomic sample analysis.PrideNot availableRaw files were analyzed using Maxquant with the following parameters. Trypsin/LysC (Maximum of 2 missed cleavage) E. coli K12 database (Uniprot UP000000625, Ver. Apr. 2022) Fixed modification: Carbamidomethylation (Cys) Variable modification: Oxidation (Met) Match-between-run LFQ (label-free quantification) for quantification. For pHis site identification, phosphorylation (Ser, The, Tyr, and His) was searched as variable modification, following the reported parameter.10 For pPyp-BP site identification, PTM change of C(23)H(22)O(6)N(3)P was searched as a variable modification for all amino acid residues. All statistical analysis was processed in the Perseus (Ver. 2.0.10.0) Other data processing was done using R (Ver. 3.6.3) within the RStudio IDE (Ver. 1.2.5042).ProteomicsJung-Min KeeAssociate professor, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST)PARTIALEscherichia Coli40201162 Choi S, Ahn S, Cho KH, Lee SK, Kee JM. Chemoproteomic identification of phosphohistidine acceptors: posttranslational activity regulation of a key glycolytic enzyme. Chem Sci. 2025 10.1039/d5sc01024aasm8430@naver.comDepartment of Chemistry, Ulsan National Institute of Science and Technology (UNIST)South KoreaHistidine phosphorylation, an unconventional and understudied posttranslational modification, often involves phosphohistidine (pHis) "acceptor" proteins, which bind to pHis residues and undergo phosphotransfer from pHis. While the roles of pHis acceptors are well-documented in bacterial cell signalling and metabolism, the presence and functions of additional pHis acceptors remain largely unknown. In this study, we introduce a chemoproteomic strategy leveraging a stable analogue of 3-pHis to identify 13 putative pHis acceptors in <i>Escherichia coli</i>. Among these, we identified phosphofructokinase-1 (PfkA), a central enzyme in glycolysis, as a pHis acceptor phosphorylated at His249 by phosphocarrier protein HPr (PtsH). This phosphorylation, modulated by carbon source availability, inhibited PfkA's kinase activity, while the pHis-specific phosphatase signal inhibitory factor X (SixA) reversed the effect, restoring the kinase function. Our findings reveal a novel regulatory mechanism in which histidine phosphorylation dynamically controls a key glycolytic enzyme, implicating a broader role for pHis in bacterial metabolism.Chemoproteomic identification of phosphohistidine acceptors: posttranslational activity regulation of a key glycolytic enzyme.Choi Solbee S, Ahn Seungmin S, Cho Kyung Hyun KH, Lee Sung Kuk SK, Kee Jung-Min JMCognitive Functions, A, Hydrogen-acceptor, Acceptor, Cognitive Function, Insights, Cognitions, Akzeptor, Functions, Insight., Function, 1-phosphono-L-histidine, Cognitive, Oxidized donor, 1-phosphohistidine, Phosphohistidine, 3-phosphohistidine, accepteurbiochemical pathways, Post Translational Amino Acid Modification, Metabolic Process, posttranslational modification, Phosphatase, Stuart-Prower Factor, Metabolic Concepts, bacterium E3, Embden-Meyerhof, Fructose-6-P 1-Kinase, Anabolism., Embden Meyerhof Parnas Pathway, L-Histidine, Protein Processing, phosphorylation, dmTAF[[II]]230, Phosphatases, Factor 10, Hydrolase, Pfkx, histidine-containing phosphocarrier protein, Roles, Carbon-12, symptoms, Concepts, ATP:D-fructose-6-phosphate 1-phosphotransferase, DL-Histidine, Embden-Meyerhof Pathway, Kinase, Enterococcus coli, Metabolism Concept, Phenomenon, phosphokinase activity, IKKg, KEY, Key, Post-Translational Protein, A, C, posttranslational amino acid modification, Escherichia/Shigella coli, TFIID TAF250, Post-Translational, cel, catabolism, E coli, Pathways, metabolic process resulting in cell growth, Posttranslational Protein Processing, Phosphomonoesterases, E. coli, Eschericia coli, Enteroaggregative Escherichia coli, signaling process, Carbon, Role Concepts, biotransformation, ATP Phosphotransferases, Catabolism, phosphatase, Autoprothrombin III, ATP, single organism signaling, 6-Phosphofructo-1-kinase, GSD7, accepteur, histidina, Blood-coagulation factor X, screening, Post-Translational Modification, dTAF[[II]]230, Pathway, Coagulation, Process, metabolism resulting in cell growth, Modifications, TAF200, anaerobic glycolysis, phosphotransfer, TAFII-250, TAF250/230, Embden-Meyerhof-Parnas, Enteroaggregative E. coli, Embden-Meyerhof-Parnas Pathway, Vitreous, AI131669, DmIKKgamma, alpha-Amino-1H-imidazole-4-propionic acid, TAFII250, Post Translational Modifications, Role Concept, Blood Coagulation Factor X, Diffusely Adherent E. coli, dIKK, Kenny, Role, secretion, HPr protein, Hydrogen-acceptor, Transphosphorylases, Diffusely Adherent Escherichia coli, PTM, Enteroinvasive E. coli, ptsH protein, 6C, Processing, L-isomer, IKK-gamma, signs, CG17603, TAF[[II]], 1-phosphohistidine, Phosphohistidine, Embden-Meyerhof pathway, L isomer, Acceptor, Vitreous Carbon, Phosphohydrolase, glycolysis, DmelCG16910, Amino Acid Modification, Taf250, post-translational modification, SR3-5, Phosphoric, carbonium, Biocatalyst, Posttranslational Modifications, Hydrolases, Embden-Meyerhof Pathways, Posttranslational Modification, TAF230, PFK-A, d230, biological signaling, Akzeptor, Post-Translational Protein Modifications, Processes, Biocatalysts, number, Histidine, Gene, dTAFII250, carbon, Metabolic Processes, Monoester Hydrolase, EfW1, presence, PFK-M, dIKK-gamma, Posttranslational, dmTAF1, Taf230, Metabolism, Escherchia coli, DmIKK-gamma, modified Embden-Meyerhof pathway, Post Translational Modification, Gene Products, Post-Translational Modifications, Enteroinvasive Escherichia coli, dmIKKgamma, IKK[[gamma]], Metabolism Phenomena, Phosphotransferase, Post Translational, TAF250, Modification, study, Taf200, dTAF[[II]]250, cell, Coagulation Factor X, Metabolic Concept, Taf1p, Histidin, Stuart Prower Factor, posttranslational protein modification, Transphosphorylase, Oxidized donor, Phosphoric Monoester Hydrolase, Protein Modifications, Post-Translational Amino Acid Modification, dTAF250, Kohlenstoff, Enzyme, IKK, Posttranslational Amino Acid Modification, Posttranslational Protein, Stuart Factor, TAF, 3-phosphohistidine, Alkalescens-Dispar Group, TAF[[II]]250, Bacterium coli, findings, Embden Meyerhof Pathway, L-isomer Histidine, degradation, ATP-PFK, Proteins, Phosphorylations, Post-Translational Protein Modification, l(3)84Ab, BG:DS00004.13, function, EAggEC, 1-phosphono-L-histidine, PFK1, Pfk4, carbone, Cell, Phosphotransferases, Concept, dTAF230, Metabolic Phenomena, carbono, Metabolism Concepts, IKKgamma, count in organism, Phosphomonoesterase, Bacillus coli, p230, PPP1R122, Protein, Phenomena, TAF[[II]]250/230, TFIID, Factor X, Protein Modification, metabolism, Metabolic Phenomenon, Taf[[II]]250, Phosphofructokinase 1, Phosphoric Monoester, multicellular organism metabolic process, PFKX, TAF[[II]]230, biodegradation, Pfka, Metabolic, Kinases, Dmikkgamma, Post Translational Protein Processing, phosphoric monoester hydrolase activity, TAF[II]250, CG16910, Embden-Meyerhof-Parnas pathway, post-translational amino acid modification, signalling, Carbon 12, Protein Gene Products, Phosphohydrolases, Gene Proteins, 6 Phosphofructokinase, Post Translational Protein Modification, DmelCG17603, Factor Ten, signalling process, Bacterium coli commune, PFKA, Pfk-4, Post-Translational Protein Processing, PFK-1, Fructose-6-phosphate 1-Phosphotransferase, TAF1, Anabolism, 6-PhosphofructokinaseAPR, Bru, posttranslational modification, E 920, anon-WO0172774.86, Raw, Hgfr, PLXN4, PLXN3, anon-WO0172774.87, INSULYSIN, DmelCG14121, leg, Aminosaeure, cleavage, 5730555F13Rik, Amino acid, anon-WO0172774.89, LFQ, Sialic acid-binding Ig-like lectin 1, insulin-degrading neutral proteinase activity, phosphorylation, l(1)AA33, std, srybeta, HGFR, insulin-degrading enzyme activity, skc35, 1, 2, sry-beta, 2-amino-3-mercaptopropanoic acid, Cystein, Enterococcus coli, insulinase activity, Svc, SER, sry alpha, C, posttranslational amino acid modification, DmelCG6127, amino acids, Escherichia/Shigella coli, MUB3_18, cisteina, M, NOXA, 2-amino-3-(4-hydroxyphenyl)propanoic acid, 3-(p-Hydroxyphenyl)alanine, insulin proteinase activity, abeta-degrading protease, insulysin, Krt-12, MUB3.18, Y, SN, E. coli, ser, Bd, c, insulinase, Sectm1, l(1)B2/13.1, RUN, Run, Tyr, HSSEXGENE, Sa, CK-12, RG7MT1, Racemethionine, 6.3, proto-oncogene c-Met, l(1)LB9, XAP-6, IDE, PRO, Aminokarbonsaeure, metalloinsulinase activity, 2-Amino-4-(methylthio)butyric acid, 2-Amino-3-(p-hydroxyphenyl)propionic acid, 9130215G10Rik, CYS, Cys, alpha-amino carboxylic acids, AA33, 1810003C24Rik, Type I keratin Ka12, Methionin, Hmet, (2R)-2-amino-3-sulfanylpropanoic acid, and GLY protein 2, region, OCAIA, HGF receptor, HGF/SF receptor, SHEP3, Amino Acid, Acid, and GLY protein 1, PTM, Amino acids, Srybeta, hCMT1c, c-Met, beta Trypsin, Sheep erythrocyte receptor, L-Cystein, data analysis, CG17957, post-translational modification, CG6127, Sry alpha, Acids, IGFBP3R, Krt1-12, CG1849, 2-amino-4-(methylsulfanyl)butanoic acid, Aminocarbonsaeure, L-Zystein, APOER, sryalpha, alpha-amino acid, CD91, HGF, mel(3)8, smooth ER, F15E12.6, Del(8)44H, RCCP2, F15E12_6, Sryalpha, Siglec-1, scatter factor receptor, E-920, Runt, sry beta, beta-Trypsin, albino, Hcys, posttranslational protein modification, AUTS9, 2-amino-4-(methylthio)butanoic acid, data processing, A2MR, CYSTEINE, CMM8, AI835216, LRP1A, FREE CYSTEINE, site, AI838057, Tyrosin, (2R)-2-amino-3-mercaptopropanoic acid, metionina, CG7938, CD169, CG5517, CG14121, LB5, insulin-specific protease activity, Bacterium coli, l(3)S147910, dIDE, dIde, LRP, DL-Methionine, DmelCG1849, srya, Phosphorylations, alpha-amino acids, Rnt, sryb, lLB5, OCA1A, insulin-glucagon protease activity, tirosina, Bacillus coli, Cytokeratin-12, K12, TGFBR5, Zystein, Data Base, ATCMPG1, MET, Met, ATCMPG2, sry-a, sry-b, 1479/10, Col4a-1, PRSS, DmelCG5517, insulin protease, Keratin-12, 2-amino-3-sulfanylpropanoic acid, tyrosine-protein kinase Met, DmelCG7938, Tripcellim, L-2-Amino-3-mercaptopropionic acid, (R)-2-amino-3-mercaptopropanoic acid, l(1)19Ea, DmelCG14121., Rpw, Amino, 2-Amino-3-mercaptopropionic acid, post-translational amino acid modification, P235, Trypure, sry-alpha, CT18858, Bacterium coli commune, alpha-amino-gamma-methylmercaptobutyric acid, insulin protease activity, E920, Par4, DmelCG17957, 5730455C01Rik, sry, SF receptorRegulations, Activity, Formal Social Control, Dmikkgamma, Biocatalysts, IKK-gamma, Control, Biocatalyst., 1-phosphono-L-histidine, Controls, CG16910, 1-phosphohistidine, Phosphohistidine, dIKK-gamma, Social Controls, Social, IKKgamma, DmIKKgamma, Enzyme, DmelCG16910, IKK, Social Control, dIKK, DmIKK-gamma, Kenny, regulation, dmIKKgamma, IKK[[gamma]], Regulation, General activity, Formal Social Controls, IKKg, KEY, Key, 3-phosphohistidineWater, sodium salt, liquid chromatography tandem mass spectroscopy, ammonium formate, 13C-labeled, cadmium salt, MeCN, PhrB photolyase activity, NCMe, ion, determination, Proteolytic Enzyme, Ass-1, Sepharose C1 4B, TFA, H(2)O, zinc formate, BOUND WATER, lead salt, SDH, CSMF, protein, Basodexan, magnesium formate, pigmented epithelium, oxidane, Strepavidin, zinc salt, heavy chain disease, WATER, HOH, Bca, Esteroproteases, peptide, SDS, Polypeptides, protein polypeptide chains, Hydrolase, ammonium tetraformate, peptido, cobalt(II) formate dihydrate, congenital, AA408052, CH3-C#N, B1, fold, AGM4, 3, Min, NUP96, Proteases, protein aggregate, epithelium, WMS, MIX, DmelCG42273, bca, Sepharose, Divorced, LCMSMS, peptides, E927b, E430039A18Rik, pigmented retina, min, chemical analysis., mAPC, proteins, CaCl2, Divorces, copper, Proteolytic, DNA cyclobutane dipyrimidine photolyase activity, Proteinases, lipomatosis of pancreas, SUPPRESSOR OF AUXIN RESISTANCE 3, AI047805, ASS, NOR-1, C1 4B, lithium salt, column, SOLO DANCERS, D1, sample, dihydridooxygen, TF, Shwachman-Bodian syndrome, stage, Lyw-57, dihydrogen oxide, GPHYSD2, ammonium (2:1) salt, Calcium chloride anhydrous, nickel salt, SGS, LC-MS2, PRE, Proteinase, CHN, cobalt (+2) salt, 4B, Trypsin/K, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, aqua, LC-MS/MS, Dmel_CG7826, AI836084, Th, Thermo Scientific, ur, collisionally activated dissociation, CID, ACMICD, SLP65, Ly-57, H2O, carbamide, sodium (4:1:1) salt, HCD, retinal pigment, magnesium salt, Dm1, Ionen, [4)-3, Sepharose C1, dipyrimidine photolyase (photosensitive), nickel (+2) salt, END, retinal pigment layer, Dmel_CG7835, Hydrogen Oxide, Mnb, MNB, parent ion, MILD1, SLP-65, ammonium salt, BASH, LY57, MS/MS, Xylella fastidiosa str. Temecula1, formate, Shwachman-Diamond type metaphyseal dysplasia, hydrogen hydroxide, cobaltous formate, cromium (+3), Shwachman syndrome, MASS, AW124434, study protocol, beta Trypsin, acqua, nanospray, phr A photolyase activity, H2NC(O)NH2, DNA-photoreactivating enzyme, precursor ion, liquid chromatography-tandem mass spectroscopy, copper salt, Sepharose 4B, ammonium (4:1) salt, Wasser, Polypeptide, methyl cyanide, lead (+2) salt, 1728, Proteolytic Enzymes, F10B6_15, nickel formate dihydrate, lead formate, developmental stage, aluminum salt, cesium salt, Pierce, FBN, Gene, Protease, Carbamide, precursor, photoreactivating enzyme activity, protein-containing complex, thomson, F10B6.15, CG7826, agua, LC-MS-MS, Buffer, formic acid, mass-to-charge ratio, Karbamid, method, potassium salt, polypeptide chain, Ly57, ECTOL1, 14C-labeled, method used in an experiment, Harnstoff, LC-MSMS, Gene Products, CG7835, CG42273, stratum pigmentosa retinae, Peptide Hydrolase, Carmol, Separated, Pro-Mega, MOS3, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, PRECOCIOUS, AI573420, thallium (+1) salt, MS2, iones, beta-Trypsin, rubidium salt, F23A5.3, 4733401P19Rik, eau, calcium formate, methanoic acid, ions, Solution, CD156, Xylella fastidiosa (strain Temecula1 / ATCC 700964), calcium chloride anhydrous, OCTD, Enzyme, lysate, congenital lipomatosis of pancreas, Agarose, peptidos, cromium (+3) salt, 6-An-alpha-L-Galp-(1->3)-beta-D-Galp-(1->]n, F23A5_3, HHT1, sodium formate, PTHB1, BLNK-S, DYRK1, NOR1, Nor1, Edg, nickel formate, 1-(14)C-labeled, 3H-labeled, protein complex, Proteins, strontium formate, potassium formate, Mischung, copper (+2) salt, strontium salt, acetonitrile, buffer, Peptide, CD142, polypeptide, MODIFIER OF SNC1, LC/MS/MS, CAD, native protein, natural protein, carbonyldiamide, C18, water, cupric formate, Protein, chemical analysis, deoxyribonucleic photolyase activity, Peptidases, MFS1, MIXL, Dyrk1, Vacuums, aluminum formate, ACETONITRILE, tandem MS, cyanomethane, WMS2, AU020952, Separation, RPE, lithium formate, PRSS, MINOR, photolyase activity, uree, Separations, CC1, Tripcellim, SWDS, Shwachman-Bodian-Diamond syndrome, ethanenitrile, CGI-97, CD156a, chromic formate, sample population, p. pigmentosa retinae, ME-IV, Protein Gene Products, plan specification, Gene Proteins, Trypure, Peptidase, Ion, concentration, Pancreatic insufficiency and bone marrow dysfunction, SSKS, [CaCl2], calcium salt, liquid chromatography tandem mass spectrometry, Peptid, TEC, deoxyribonucleate pyrimidine dimer lyase (photosensitive), assay, ORW1, [OH2], MinorForms, Regulations, PFK-A, Activity, Biocatalysts, Phosphatase, Histidine, Gene, bacterium E3, Embden-Meyerhof, eubacteria, protein, Fructose-6-P 1-Kinase, Monoester Hydrolase, protein-containing complex, Embden Meyerhof Parnas Pathway, L-Histidine, phosphorylation, PFK-M, dIKK-gamma, Social Controls, protein amino acid phosphorylation, Phosphatases, protein polypeptide chains, Hydrolase, Pfkx, polypeptide chain, Roles, Escherchia coli, DmIKK-gamma, modified Embden-Meyerhof pathway, Gene Products, symptoms, ATP:D-fructose-6-phosphate 1-phosphotransferase, Concepts, DL-Histidine, Embden-Meyerhof Pathway, Enteroinvasive Escherichia coli, Bacteriobiota, Enterococcus coli, dmIKKgamma, IKK[[gamma]], protein aggregate, prevention and control, Formal Social Controls, IKKg, KEY, Key, study, Eubacteria, Escherichia/Shigella coli, enzymes, reference sample, E coli, Pathways, prevention., Histidin, proteins, Phosphomonoesterases, Monera, E. coli, Phosphoric Monoester Hydrolase, Bacteria Woese et al. 2024, Social, Bacteria (ex Cavalier-Smith 1987), Eschericia coli, Enteroaggregative Escherichia coli, preventive measures, Enzyme, Bacteria <bacteria>, IKK, Prokaryotae, Role Concepts, fungi, Procaryotae, phosphatase, 6-Phosphofructo-1-kinase, GSD7, Controlled, 3-phosphohistidine, Alkalescens-Dispar Group, histidina, screening, bacteria, Controlling, Bacterium coli, Pathway, preventive therapy, findings, Embden Meyerhof Pathway, L-isomer Histidine, Formal Social Control, ATP-PFK, protein complex, Proteins, Phosphorylations, anaerobic glycolysis, enzyme activity, EAggEC, 1-phosphono-L-histidine, PFK1, Pfk4, Embden-Meyerhof-Parnas, Enteroaggregative E. coli, Concept, Embden-Meyerhof-Parnas Pathway, IKKgamma, AI131669, DmIKKgamma, Phosphomonoesterase, alpha-Amino-1H-imidazole-4-propionic acid, Role Concept, native protein, natural protein, Social Control, Diffusely Adherent E. coli, Bacillus coli, dIKK, PPP1R122, Protein, Kenny, Role, prokaryotes, Phosphofructokinase 1, Phosphoric Monoester, Diffusely Adherent Escherichia coli, PFKX, Enteroinvasive E. coli, Pfka, Dmikkgamma, prophylaxis, L-isomer, IKK-gamma, Control, signs, phosphoric monoester hydrolase activity, Controls, CG16910, Embden-Meyerhof-Parnas pathway, 1-phosphohistidine, Phosphohistidine, Embden-Meyerhof pathway, L isomer, Protein Gene Products, Phosphohydrolases, Gene Proteins, 6 Phosphofructokinase, Phosphohydrolase, DmelCG16910, glycolysis, Bacterium coli commune, PFKA, control, prokaryote, Pfk-4, Phosphoric, Biocatalyst, PFK-1, Fructose-6-phosphate 1-Phosphotransferase, regulation, Hydrolases, Regulation, General activity, Embden-Meyerhof Pathways, Prokaryota, 6-PhosphofructokinasefalseChemoproteomic identification of a phosphohistidine acceptor: Insights into posttranslational regulation of glycolysisHistidine phosphorylation is an underexplored form of protein phosphorylation. Despite the widespread existence of phosphohistidine (pHis) sites, particularly in bacteria, their phosphorylation regulation and physiological functions remain poorly understood. In this study, we developed a chemoproteomic strategy employing a stable pHis analog to identify pHis-recognizing proteins in Escherichia coli. Our probe successfully labeled known pHis-recognizers and revealed many putative pHis acceptors, including phosphofructokinase-1 (PfkA), a key glycolytic enzyme. We demonstrated that PfkA undergoes histidine phosphorylation at His249 mediated by the phosphocarrier protein PtsH, thereby reducing enzyme activity. This phosphorylation was reversed by the pHis-specific phosphatase SixA, which restored the PfkA activity. Our findings reveal a novel posttranslational regulatory mechanism affecting glycolysis, implicating a broader role of histidine phosphorylation in bacterial metabolic control.2025-05-072024-06-11PXD053006NEWT:330879NEWT:377960NEWT:2042546NEWT:259447NEWT:295546NCBITaxon:2719036NEWT:625351NCBITaxon:1280NEWT:112503NEWT:1129NEWT:309807NEWT:59753NEWT:89184NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:193516NEWT:1073882NEWT:6819NEWT:475174NEWT:111205NEWT:1117NEWT:498257NEWT:10036NEWT:1590NEWT:10034NEWT:661410NEWT:638632NEWT:224326NEWT:376619NCBITaxon:79857NEWT:1096976NEWT:3765NEWT:1589NEWT:135622NEWT:35786NEWT:1580NEWT:399784NEWT:96731NEWT:656064NEWT:1582NEWT:36630NEWT:383379NEWT:418106NCBITaxon:12721NEWT:10029NEWT:913645NEWT:641809NEWT:1392488NEWT:38815NEWT:317447NEWT:4688NEWT:111225NEWT:7719NEWT:868565NEWT:135674NEWT:79329NEWT:30069NEWT:12637NEWT:59729NEWT:2164133NEWT:295105NEWT:108061NEWT:60711NEWT:224308NEWT:3347NEWT:160621NEWT:212790NEWT:110368NEWT:1310161NEWT:77133NEWT:145481NEWT:1310165NEWT:29058NCBITaxon:79824NEWT:410658NEWT:44688NEWT:44689NEWT:498211NEWT:347256NEWT:5518NEWT:398007NEWT:1527468NEWT:498217NEWT:498216NEWT:11320NEWT:246196NEWT:246197NEWT:145458NEWT:44685NEWT:161934NEWT:156587NEWT:1148NEWT:5508NEWT:3329NEWT:419481NEWT:5507NEWT:410661NEWT:1140NCBITaxon:2157NEWT:1143NEWT:1287689NEWT:1094343NEWT:1462472NEWT:1336795NEWT:644042NEWT:1182590NEWT:3712NEWT:3711NEWT:3714NEWT:270643NEWT:2065263NEWT:177437NEWT:10418NEWT:118698NEWT:1616117NEWT:118696NEWT:34865NEWT:52283NEWT:28174NEWT:284812NEWT:8175NEWT:43330NEWT:1603293NEWT:44664NEWT:43346NEWT:3702NEWT:1245466NEWT:3704NEWT:244366NEWT:1246791NEWT:176597NEWT:118694NEWT:2850NEWT:118691NEWT:34871NEWT:33548NEWT:52299NEWT:8165NEWT:96794NEWT:3708NEWT:332648NEWT:44670NEWT:536231NEWT:376219NEWT:219813NEWT:1510NEWT:460519NEWT:1515NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:1348799NEWT:33995NEWT:55784NEWT:1303443NEWT:9483NEWT:485NEWT:56636NEWT:2499525NEWT:2853422NEWT:487NEWT:1679718NEWT:480NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:1185650NEWT:216778NEWT:294607NEWT:1502NEWT:128017NEWT:376686NEWT:237610NEWT:95486NEWT:1883446NEWT:1233435NEWT:118223NEWT:11757NEWT:109760NEWT:29031NEWT:317047NEWT:491NEWT:460523NEWT:235443NEWT:1757312NEWT:85694NEWT:109786NEWT:108458NEWT:5936NEWT:320637NEWT:1078283NEWT:3750NEWT:983964NEWT:11706NEWT:32644NEWT:527796NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:3747NEWT:1116234NEWT:1255228NEWT:410289NEWT:373153NEWT:1640278NEWT:472NEWT:940825NEWT:529507NEWT:1071661NEWT:28128NEWT:470NEWT:5911NEWT:748693NCBITaxon:50557NEWT:39251NEWT:29491NEWT:101841NEWT:446NEWT:153481NEWT:2014887NEWT:33952NEWT:445NEWT:153009NEWT:261756NEWT:166313NEWT:63366NEWT:63367NEWT:215402NEWT:1547NEWT:906914NEWT:9031NEWT:27292NEWT:1050722NEWT:3728NEWT:108931NEWT:1293497NEWT:1055524NEWT:150475NEWT:267872NEWT:381666NEWT:172269NEWT:9534NEWT:5180NEWT:256737NEWT:9541NEWT:8694NEWT:33936NEWT:8692NEWT:2903NEWT:185579NEWT:13076NEWT:207340NEWT:1006581NEWT:8673NEWT:33940NEWT:550NEWT:554NEWT:451516NEWT:552NEWT:1325291NEWT:36185NEWT:1054211NEWT:1225786NEWT:771870NEWT:51338NEWT:575412NEWT:28112NEWT:6493NEWT:10832NEWT:6494NEWT:6491NEWT:507601NEWT:520NEWT:186441NEWT:643680NEWT:214092NCBITaxon:6157NEWT:13095NEWT:162425NEWT:104105NEWT:216257NEWT:9986NEWT:8654NEWT:8658NEWT:1268063NEWT:8657NEWT:9983NEWT:8655NEWT:5147NEWT:28108NEWT:28104NEWT:407821NEWT:568703NCBITaxon:2NEWT:568708NEWT:1613NEWT:986NEWT:52641NEWT:28532NEWT:353152NEWT:298176NEWT:353153NEWT:227377NEWT:1774284NEWT:40674NEWT:1194669NEWT:51329NEWT:443906NEWT:519NEWT:2510939NEWT:7394NEWT:6063NEWT:1328388NEWT:105487NEWT:1548728NEWT:667127NEWT:9557NEWT:377586NEWT:300641NEWT:39655NEWT:9554NEWT:38323NEWT:475932NEWT:256318NEWT:206411NCBITaxon:6191NEWT:229533NEWT:214053NEWT:28550NEWT:80863NEWT:90675NEWT:52639NEWT:52638NEWT:57075NEWT:1666905NEWT:8697NEWT:8695NEWT:884204NEWT:510516NEWT:1045775NEWT:1123869NEWT:9544NEWT:9545NEWT:979NEWT:7370NEWT:83906NEWT:99488NEWT:1134506NEWT:255470NEWT:38783NEWT:6426NEWT:33090NEWT:9935NEWT:287889NEWT:305959NEWT:1028307NEWT:13489NEWT:29829NEWT:92867NEWT:92866NCBITaxon:3055NEWT:51750NEWT:202950NEWT:441772NEWT:1293091NEWT:48106NEWT:202956NEWT:295027NCBITaxon:11320NEWT:632957NEWT:9925NCBITaxon:9606NEWT:90690NEWT:1436183NEWT:300273NEWT:4232NEWT:416348NEWT:11298NEWT:4236NEWT:7742NEWT:196627NEWT:200308NEWT:200302NEWT:870435NEWT:9913NEWT:9915NEWT:105841NEWT:2666255NEWT:999810NCBITaxon:5693NEWT:28995NEWT:2144189NEWT:1392998NEWT:380394NEWT:1150621NEWT:226900NEWT:1266738NEWT:231490NEWT:244704NEWT:7725NEWT:9909NEWT:9902NEWT:430615NEWT:563041NEWT:72664NEWT:36599NEWT:41949NEWT:249161NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1678078NEWT:749906NEWT:418985NEWT:749907NEWT:150847NEWT:29835NEWT:431947NEWT:69014NEWT:142809NEWT:302409NEWT:130821NEWT:27606NEWT:1519788NEWT:59202NEWT:9975NEWT:8643NEWT:1801631NEWT:1159899NEWT:502780NEWT:860688NEWT:13443NEWT:35231NEWT:8644NEWT:1125630NEWT:5141NEWT:46360NCBITaxon:3044782NEWT:202914NEWT:160235NEWT:502779NEWT:297246NEWT:8639NEWT:1928434NEWT:8637NEWT:1114970NEWT:2096NEWT:285272NEWT:192222NEWT:9963NEWT:1617910NEWT:197911NEWT:197912NEWT:637379NEWT:12107NEWT:2785785NEWT:411901NEWT:129872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