{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Csv":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD060441/F1Co_results.csv","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD060441/protein-peptides.csv","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD060441/F1P_results.csv","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD060441/protein_peptides_Control.csv"],"Raw":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD060441/Ctr_Paulo.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD060441/F1_Paulo.raw"],"Other":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD060441/F1IP_S1-A3_1_1728.d.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD060441/F1Co_S1-A5_1_1730.d.zip"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"labhead_mail":["jischroeder@ucsd.edu"],"submitter":["Majid Ghassemian"],"technology_type":["Data-dependent acquisition","Mass Spectrometry","Bottom-up proteomics"],"software":["Not available"],"submitter_keywords":["Aba","Arabidopsis","Signalling"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD060441"],"tissue":["Leaf"],"sample_protocol":["IP-MS Assay  Total protein extracts from the leaves of His-FIA1decoy-FLAG transgenic  Arabidopsis plants were prepared by homogenizing in liquid nitrogen and  resuspending in a binding buffer, as described (Lee et al., 2018). Briefly, the binding  buffer consisted of 50 mM Tris-HCl (pH 7.5), 150 mM sodium chloride (NaCl), 5 mM  magnesium chloride (MgCl₂), 10% glycerol, 0.1% NP-40, 0.5 mM dithiothreitol (DTT),  with complete ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor  cocktail and 1 mM phenylmethylsulfonyl fluoride (PMSF). Cell debris was removed  by centrifugation, and the supernatant was pre-cleared using IgG-conjugated beads.  Supernatant was then incubated with Ni-NTA beads for 3 hours at 4°C on a tube  rocker followed by extensive washes with binding buffer. The immuno-precipitation of  FIA1 proteins was validated by anti-His immuno-blotting (Supplementary figure 2).  6"],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["The protein sequence information and the post translational modifications was analysed usingPeaks Studio 8.5"],"omics_type":["Proteomics"],"labhead":["Julian Schroeder"],"instrument_platform":[""],"labhead_affiliation":["Cell and Developmental Biology Department, School of Biological Sciences,  University of California, San Diego, La Jolla, CA, USA"],"submission_type":["PARTIAL"],"species":["Arabidopsis Thaliana (mouse-ear Cress)"],"publication":["40439299 Kasera M, Ceciliato PHO, Lopez BNK, Hauser F, Gendron JM, Schroeder JI. Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination. Philos Trans R Soc Lond B Biol Sci. 2025 380(1927):20240233 10.1098/rstb.2024.0233"],"submitter_mail":["mghassem@ucsd.edu"],"submitter_affiliation":["Scientist"],"submitter_country":["United States"],"pubmed_abstract":["Large gene families and the frequent overlapping functions of homologous genes remain a major challenge for functional forward genetic screens in plants. The recent development of homology-based gene silencing using computationally generated artificial microRNAs (amiRNAs) has been demonstrated to be a promising tool for unbiased functional genomics in plants by circumventing redundancies and lethality. In this study, through a forward genetics screen, we isolated an abscisic acid (ABA)-insensitive amiRNA line targeting five previously uncharacterized <i>F-box Insensitive to ABA</i> (<i>FIA</i>) genes. Notably, a triple mutant in the identified <i>FIA</i> genes <i>FIA1</i>, <i>FIA3</i> and <i>FIA4</i> (<i>f1/f3/f4</i>), that are expressed in germinating seeds, exhibited insensitivity in ABA inhibition of seed germination. In contrast, this ABA insensitivity was not observed in a double mutant of two <i>FIA</i> genes <i>FIA1</i> and <i>FIA4</i>. Further investigation of the FIA1 interactome using F-box decoy lines revealed the gibberellin (GA)-responsive GASA1 protein that has been reported to encode a small stress signalling peptide as an interacting partner. We found that ABA promoted the ubiquitination of GASA1 in <i>Arabidopsis</i>, leading to its degradation via the 26S proteasome pathway. Together, our study reveals that ABA represses seed germination through FIA proteins and regulates the FIA interactor, GA-responsive GASA1.This article is part of the theme issue 'Crops under stress: can we mitigate the impacts of climate change on agriculture and launch the 'Resilience Revolution'?'."],"pubmed_title":["Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination."],"pubmed_authors":["Kasera Mritunjay M, Ceciliato Paulo H O PHO, Lopez Bryn N K BNK, Hauser Felix F, Gendron Joshua M JM, Schroeder Julian I JI"],"additional_accession":[]},"is_claimable":false,"name":"Identification of F-Box Proteins in ABA-regulated Seed Germination:  Interaction of GASA1 signaling peptide and ABA-Induced Ubiquitination in  Arabidopsis","description":"Large gene families and the frequent overlapping functions of homologous genes  remain a major challenge for functional genetics in plants. In recent years, the  development of homology-based gene silencing using computationally generated  artificial microRNAs (amiRNAs) has been demonstrated to be a promising tool for  functional genomics in plants. In this study, through a forward genetics screen, we  isolated an abscisic acid-insensitive amiRNA line targeting five previously  uncharacterized F-box Insensitive to ABA (FIA) genes. Notably, a triple mutant in the  identified FIA genes FIA1, FIA3 and FIA4 (f1/f3/f4), that are expressed in  germinating seeds, exhibited ABA insensitivity in the inhibition of seed germination.  In contrast, this ABA insensitivity was not observed in a double mutant of two FIA  genes FIA1 and FIA4 (f1/f4). Further investigation of the FIA1 interactome using F box decoy lines revealed the gibberellin (GA)-responsive GASA1 protein that has  been reported to encode a small stress signaling peptide as an interacting partner.  We found that abscisic acid promoted the ubiquitination of GASA1 in Arabidopsis,  leading to its degradation via the 26S proteasome pathway. Together, our study  reveals that abscisic acid represses seed germination through FIA proteins and  regulates the FIA interactor, GA-responsive 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