Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/TAIR_13112024_DIANN.fastaftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S41_ELechner_col-2-1DIA_6725.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2020S28_ELechner_FBL17-3DIA_6698.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2020S28_ELechner_FBL17-1DIA_6696.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_col2-3DIA_6738.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_FBL17-1-3DIA_6686.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_FBL17-2-3DIA_6692.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/DIANN.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_FBL17-1-1DIA_6683.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S41_ELechner_FBL17-2-1DIA_6677.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S41_ELechner_FBL17-1-1DIA_6676.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_FBL17-2-1DIA_6690.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2020S28_ELechner_FBL17-2DIA_6697.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_col2-1DIA_6736.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_col1-1DIA_6731.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_FBL17-1-2DIA_6705.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_col1-3DIA_6733.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_col1-2DIA_6732.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_col2-2DIA_6737.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S37_ELechner_FBL17-2-2DIA_6691.d.7zftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063507/2024S41_ELechner_col-1-1DIA_6724.d.7zprimaryOK200pascal.genschik@ibmp-cnrs.unistra.frJohana ChicherAffinity purification coupled with mass spectrometry proteomicsMass SpectrometrydiaPASEFNot availableUbiquitinPlantF-boxCell cycleE2f/rbrhttps://www.ebi.ac.uk/pride/archive/projects/PXD063507SeedlingFor immunoprecipitation of FBL17-GFP, 0.33gg of frozen plant material (10 day-old seedlings previously treated in 25µM MLN-4924 for 16 to 24 hours) were ground to a fine powder with mortar and pestle, resuspended in 3 volumes of IP Extraction Buffer (25mM Tris HCl, pH 7.5, 75mM NaCl, 15mM MgCl2, 0.15mM EGTA, 25µM MLN-4924, 60mM p-glycerophosphate, 1mM DTT, 0.1% Igepal CA, 0.1mM Na3VO3, 0.5 mM NaF, and 1 cOmpleteTM Protease Inhibitor Cocktail [Roche], 1pg/mL each leupeptin and aprotinin). For the formaldehyde IPs, 50 mM HCL-free Tris was used in the buffer with a final formaldehyde concentration of 0.375%. An additional step of glycine 200 mM final was added for the quenshing step of the bridging agent. Insoluble material was removed by centrifugation (twice, 15 min, 12 000g, 4°C). Identical amounts of crude extracts were incubated with 50µL anti-GFP magnetic beads (Anti-GFP Microbeads from µMACS GFP Isolation kit; Miltenyi) for 30mn at 8 rpm in the cold room. Immune complexes were washed four times in the crude extract buffer, and purified protein complexes were eluted from the beads in the Elution buffer following the manufacturer’s instructions. Eluted proteins were reduced (5 mM DTT), alkylated (10 mM iodoacetamide) and digested with 300 ng tryspin. Generated peptides were analysed by nanoLC-MS/MS on a reversed phase nanoElute 2 coupled to a TIMS-TOF Pro 2 mass spectrometer (Bruker Daltonik Gmbh) using a data-independent acquisition and parallel accumulation-serial fragmentation (DIA PASEF). Peptides were separated on an IonOpticks Aurora Elite column (25 cm × 75μM, 1.7μm particle size and 120 Å pore size; AUR3–15075C18-CSI) with 45 min gradients.PrideData were searched against the TAIR v10 database using DIANN 1.9 software with a library free approach.ProteomicsPascal GenschikInstitut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, 12, rue du Général Zimmer, 67084 Strasbourg, FrancePARTIALArabidopsis Thaliana (mouse-ear Cress)j.chicher@ibmc-cnrs.unistra.fr41706863 Espanet J, He X, Pan T, Gentric N, Potuschak T, Desvoyes B, Hammann P, Chicher J, Brik R, Lechner E, Latrasse D, Pettkó-Szandtner A, Gutierrez C, Raynaud C, Magyar Z, Benhamed M, Yan S, Noir S, Genschik P. Tuning of the RBR1-E2F/DP transcriptional module by the F-box protein FBL17. Sci Adv. 2026 12(8):eadz2439 10.1126/sciadv.adz2439Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche ScientifiqueFrancefalseRBR1-E2F/DP transcriptional regulation modulated by the F-box protein FBL17In all eukaryotes, F-box proteins of SCF E3 ligase complexes have been well-documented to control the abundance of numerous critical regulatory proteins. In Arabidopsis, one of them, F-BOX-LIKE17 (FBL17), stands out for playing a key role in DNA replication, DNA damage and more recently for the control of cell size. FBL17 null mutants exhibit severe cellular defects leading to lethality, presumably due to impaired degradation of cyclin-dependent kinase inhibitor proteins. However, the molecular mechanisms by how FBL17 operate remain poorly understood. Here we show that FBL17 interacts with different components of the RETINOBLASTOMA-RELATED1/E2F module. In particular, FBL17 directly targets the two transcription factors E2Fa and E2Fb and is involved in their protein turnover. However, it is the transcriptional repressor E2Fc, which is not a substrate of FBL17, that causes fbl17 mutant lethality. Hence, the e2fc mutation broadly suppresses cell elongation and cell proliferation defects observed in fbl17. Moreover, in the fbl17 e2fb double mutant the strong overexpression of DNA damage genes is significantly attenuated through RBR1 recruitment to its target genes. Altogether, our results highlight a key role for FBL17 in modulating the transcriptional control of E2F-target genes ensuring precise control of cell cycle progression and avoiding uncontrolled DNA damage response.2026-02-232025-04-30PXD063507NEWT:6945NEWT:3555NEWT:241368NEWT:2NEWT:157546NEWT:190802NEWT:35554NEWT:150475NEWT:9417NEWT:347515NEWT:1216979NEWT:307972NEWT:32046NEWT:544496NEWT:5180NEWT:256737NEWT:115104NEWT:1081927NEWT:67825NEWT:13076NEWT:1249668NEWT:317NEWT:1736309NEWT:7227NEWT:7469NEWT:885318NEWT:876138NEWT:4081NEWT:554NEWT:98334NEWT:237561NEWT:10036NEWT:7574NEWT:1351NEWT:7215NEWT:272563NEWT:507601NCBITaxon:79857NCBITaxon:6157NEWT:95648NEWT:746360NEWT:6239NEWT:1589NEWT:470150NEWT:135622NEWT:216257NEWT:6915NEWT:9986NEWT:101510NEWT:4054NEWT:3880NEWT:8782NEWT:1000589NEWT:1902NEWT:85962NEWT:160488NEWT:28104NEWT:317447NEWT:7955NCBITaxon:2NEWT:985076NEWT:7959NEWT:2261NEWT:4565NEWT:1264690NEWT:6192NEWT:28532NCBITaxon:38727NEWT:34305NEWT:59729NCBITaxon:183674NEWT:224308NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:216595NEWT:243230NEWT:8355NEWT:931281NEWT:7029NEWT:1283300NEWT:334747NEWT:61235NCBITaxon:79824NCBITaxon:4563NEWT:5755NEWT:3218NEWT:5759NEWT:1736231NEWT:436486NEWT:6287NEWT:2242NEWT:300641NEWT:4784NEWT:727NEWT:9796NEWT:725NEWT:360106NEWT:260707NEWT:287NEWT:10117NEWT:10239NCBITaxon:6191NEWT:10116NEWT:1280NEWT:1836NEWT:1735272NEWT:83334NEWT:83332NEWT:29760NEWT:260704NEWT:703612NEWT:260705NEWT:80863NEWT:44685NEWT:2697049NEWT:1148NEWT:11676NEWT:55571NEWT:100226NCBITaxon:6073NEWT:4530NEWT:4896NEWT:6279NEWT:1123869NEWT:7370NEWT:6282NEWT:1134506NEWT:575584NEWT:1773NEWT:38783NEWT:8727NEWT:1895NEWT:1182590NEWT:8726NEWT:10090NEWT:935293NEWT:749200NEWT:4120NEWT:5693NEWT:8724NEWT:51511NEWT:92867NEWT:8723NEWT:990346NEWT:5334NEWT:145953NEWT:257309NEWT:230741NEWT:284812NCBITaxon:10359NCBITaxon:1313NEWT:43330NEWT:242619NEWT:44544NEWT:373995NEWT:544404NEWT:3702NEWT:129249NEWT:8839NEWT:4232NEWT:990119NEWT:2758385NEWT:4113NEWT:837NEWT:11298NEWT:171101NEWT:196627NEWT:408172NEWT:5691NEWT:408170NEWT:493760NEWT:260710NEWT:627025NEWT:400772NEWT:1097677NEWT:3708NEWT:106592NEWT:1117957NEWT:9913NEWT:1432138NEWT:10312NEWT:4100NEWT:1076NEWT:6763NEWT:803NEWT:8030NEWT:29722NEWT:380394NEWT:1692259NEWT:1639NEWT:188229NEWT:3818NEWT:480NEWT:4909NEWT:180066NEWT:67767NEWT:46835NEWT:135588NEWT:1843183NEWT:95486NEWT:58002NEWT:9103NEWT:4577NEWT:5664NEWT:2157NEWT:146479NEWT:10306NEWT:1911079NEWT:8022NEWT:145943NEWT:3635NEWT:5811NEWT:1480154NEWT:235443NEWT:1274414NEWT:3197NEWT:9615NEWT:10299NEWT:860688NEWT:884019NEWT:169963NEWT:36329NEWT:9606NEWT:367830NEWT:157295NEWT:410289NEWT:373153NEWT:915099NEWT:74940NEWT:1450511NEWT:470NEWT:84023NEWT:9838NCBITaxon:9615NEWT:1193501NEWT:3055NEWT:6326NEWT:6689NEWT:2762NEWT:5476NEWT:1174673NEWT:562NEWT:33952NEWT:1274432NEWT:1274426NEWT:1423NEWT:4932NEWT:70448NEWT:9825NEWT:1274423NEWT:3603NEWT:698936NEWT:2759NEWT:3847NEWT:39946NEWT:9823NEWT:9940NEWT:573NEWT:9031NEWT:1274420NEWT:7091417068630000-0002-4403-7484