Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20190429_QE25CM114_pl19038_KBellofatto_01.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20210408_QEHF25CM216_pl21052_KB_04.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20210408_QEHF25CM218_pl21052_KB_05.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20210408_QEHF25CM214_pl21052_KB_03.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20190429_QE25CM116_pl19038_KBellofatto_02.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20210408_QEHF25CM216_pl21052_KB_04__F013783_.mzid_20210408_QEHF25CM216_pl21052_KB_04__F013783_.MGFftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20210408_QEHF25CM216_pl21052_KB_04__F013783_.mzidftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20190429_QE25CM114_pl19038_KBellofatto_01__F010288_.mzid_20190429_QE25CM114_pl19038_KBellofatto_01__F010288_.MGFftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20210408_QEHF25CM214_pl21052_KB_03__F013782_.mzid_20210408_QEHF25CM214_pl21052_KB_03__F013782_.MGFftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20190429_QE25CM114_pl19038_KBellofatto_01__F010288_.mzidftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20210408_QEHF25CM218_pl21052_KB_05__F013784_.mzidftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20210408_QEHF25CM214_pl21052_KB_03__F013782_.mzidftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20190429_QE25CM116_pl19038_KBellofatto_02__F010289_.mzid_20190429_QE25CM116_pl19038_KBellofatto_02__F010289_.MGFftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20190429_QE25CM116_pl19038_KBellofatto_02__F010289_.mzidftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063772/20210408_QEHF25CM218_pl21052_KB_05__F013784_.mzid_20210408_QEHF25CM218_pl21052_KB_05__F013784_.MGFprimaryOK200ekaterine.berishvili@unige.chFanny LebretonData-dependent acquisitionMass SpectrometryHumanBioartificial pancreashttps://www.ebi.ac.uk/pride/archive/projects/PXD063772PlacentaSamples were homogenized in RIPA buffer (25ug / 100 uL). They were stored at -20°C. The Mass Spectrometry was performed by the Proteomics Core Facility, Faculty of Medicine, University of Geneva, Switzerland. Sample preparation: 200 μl (50 ug) of each protein sample was centrifuged and the RIPA fraction was discarded. Proteins pellets were resuspended in 100 ul of thiourea/urea lysis buffer (ammonium bicarbonate 50 mM, 5.0M urea, 2.0M thiourea, 50mM DTT, 0.1% SDS) for 3 h at room temperature, followed by overnight acetone precipitation. The mixture was centrifuged 10 min. at 15000 rpm and pellets were washed three times with acetone. Pellets were re-suspended in 100 μl of 8M urea in 50 mM ammonium bicarbonate (AB). 2 μl of Dithioerythritol (DTE) 50 mM in distilled water were added and the reduction was carried out at 37°C for 1h. Alkylation was performed by adding 2 μl of iodoacetamide (400 mM in distilled water) during 1 hour at room temperature in the dark. Urea concentration was reduced to 1M by addition of 700 μl of AB and overnight digestion was performed at 37 °C with 5-10 μL of freshly prepared trypsin (Promega) at 0.1 μg/μl in AB. Supernatants were collected and completely dried under speed-vacuum. Samples were then desalted with a C18 microspin column (Harvard Apparatus, Holliston, MA, USA) according to manufacturer’s instructions, completely dried under speed-vacuum and stored at -20°C. ESI-LC-MSMS: Sample was diluted in 30 μl of loading buffer (5% CH3CN, 0.1% FA) and 1 μl was injected on column. LC-ESI-MS/MS was performed on a Q-Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) equipped with an Easy nLC 1000 liquid chromatography system (Thermo Fisher Scientific). Peptides were trapped on a Acclaim pepmap100, C18, 3μm, 75μm x 20mm nano trap-column (Thermo Fisher Scientific) and separated on a 75 μm x 250 mm, C18, 2μm, 100 Å Easy-Spray column (Thermo Fisher Scientific). The analytical separation was run for 90 min using a gradient of H2O/0.1 % Formic acid (solvent A) and CH3CN/0.1 % Formic acid (solvent B). The gradient was run as follows: 0-5 min 95 % A and 5 % B, then to 65 % A and 35 % B for 60 min, then to 10 % A and 90 % B for 10 min and finally stay 15 min at 10 % A and 90 % B. Flow rate was of 250 nL/min for a total run time of 90 min. For MS survey scans, the resolution was set to 70’000 and the ion population was set to 3 × 106 with an m/z window from 400 to 2000. For data dependant analysis, up to fifteen precursor ions were isolated and fragmented by higher-energy collisional dissociation HCD at 27% NCE. For MS/MS detection, the resolution was set to 17’500, the ion population was set to 1 × 105 with an isolation width of 1.6 m/z units.PrideDatabase search: Peak lists (MGF file format) were generated from raw data using the MS Convert conversion tool from ProteoWizard. The peaklist files were searched against the Human Reference Proteome database (Uniprot, 2018-06, 21044 entries) combined with an in-house database of common contaminant using Mascot (Matrix Science, London, UK; version 2.5.1). Trypsin was selected as the enzyme, with one potential missed cleavage. Precursor ion tolerance was set to 10 ppm and fragment ion tolerance to 0.02 Da. Carbamidomethyl of cysteine was specified as fixed modification. Deamidation of asparagine and glutamine as well as oxidation of methionine were specified as variable modifications. Scaffold (version Scaffold_5.3.3, Proteome Software Inc., Portland, OR) was used to validate MS/MS based peptide and protein identifications. Peptide identifications were accepted if they could be established at greater than 95,0% probability. Peptide Probabilities from Mascot (samples 20190429_QE25CM114_pl19038_KBellofatto_01 (F010288) and 20190429_QE25CM116_pl19038_KBellofatto_02 (F010289)) were assigned by the Peptide Prophet algorithm (Keller, A et al Anal. Chem. 2002;74(20):5383-92) with Scaffold delta-mass correction. Peptide Probabilities from Mascot (samples 20210408_QEHF25CM214_pl21052_KB_03 (F013782), 20210408_QEHF25CM216_pl21052_KB_04 (F013783) and 20210408_QEHF25CM218_pl21052_KB_05 (F013784)) were assigned by the Percolator posterior error probability calculation (Käll, L et al, Bioinformatics, 24(16):i42-i48, Aug 2008). Protein identifications were accepted if they could be established at greater than 95,0% probability and contained at least 2 identified peptides. Protein probabilities were assigned by the Protein Prophet algorithm (Nesvizhskii, Al et al Anal. Chem. 2003;75(17):4646-58). Proteins that contained similar peptides and could not be differentiated based on MS/MS analysis alone were grouped to satisfy the principles of parsimony.ProteomicsEkaterine BerishviliLaboratory of Tissue Engineering and Organ Regeneration, Department of Surgery, University of Geneva, Geneva, SwitzerlandCOMPLETEHomo Sapiens (human)fanny.lebreton@unige.chNot availableHopitaux Universitaires de GenèveSwitzerland10.6019/PXD063772falseBioengineering of the implantable vascularized endocrine constructs for insulin delivery suitable for clinical upscalingBeta cell replacement therapy for type 1 diabetes is hindered by poor graft survival and suboptimal function, largely due to inadequate vascularization and lack of supportive microenvironment. To address these challenges, we developed a clinically scalable, extracellular matrix (ECM)–mimetic hydrogel, termed Amniogel, derived from human amniotic membrane via streamlined, clinically compliant process. Co-encapsulation of pancreatic islets with blood outgrowth endothelial cells (BOECs) within Amniogel facilitated the formation of prevascularized endocrine constructs (VECs).2026-04-242025-05-08PXD063772NEWT:3555NEWT:71647NEWT:35554NEWT:32046NEWT:544496NEWT:2042546NEWT:32049NEWT:1392696NEWT:259447NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:5722NCBITaxon:1280NEWT:1129NEWT:309807NEWT:55153NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:10036NEWT:1590NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:1147161NEWT:224326NEWT:1333499NCBITaxon:79857NEWT:1096976NEWT:95648NEWT:1589NEWT:135622NEWT:1349NEWT:96731NEWT:383379NEWT:10029NEWT:913645NEWT:44491NEWT:556484NEWT:317447NEWT:4688NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:3112NEWT:4442NEWT:192875NEWT:12637NEWT:59729NEWT:2164133NEWT:108061NEWT:1316931NEWT:224308NEWT:3347NEWT:511145NEWT:931281NEWT:658457NEWT:1310161NEWT:77133NEWT:295358NEWT:145481NCBITaxon:79824NCBITaxon:4563NEWT:5755NEWT:44689NEWT:44447NEWT:5759NEWT:1736231NEWT:1392NEWT:498217NEWT:2242NEWT:11320NEWT:286NEWT:391619NEWT:246196NEWT:287NEWT:246197NEWT:633149NEWT:10239NEWT:44685NEWT:161934NEWT:1148NEWT:5508NEWT:5507NEWT:410661NEWT:55571NEWT:35500NEWT:1140NCBITaxon:2157NEWT:1143NEWT:4896NEWT:1390NEWT:11557NEWT:1094343NEWT:1336795NEWT:644042NEWT:294NEWT:1773NEWT:1182590NEWT:3712NEWT:82380NEWT:935293NEWT:375146NEWT:118698NEWT:263NEWT:118696NEWT:52283NEWT:284812NEWT:8175NEWT:43330NEWT:1603293NEWT:408169NEWT:44664NEWT:47946NEWT:3702NEWT:243277NEWT:7067NEWT:118694NEWT:2850NEWT:118691NEWT:33548NEWT:274NEWT:408172NEWT:408170NEWT:3708NEWT:332648NEWT:44670NEWT:536231NEWT:376219NEWT:1436733NEWT:460519NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:1513458NCBITaxon:40559NEWT:84588NEWT:1679718NEWT:480NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:300852NEWT:1502NEWT:376686NEWT:95486NEWT:508771NEWT:1883446NEWT:253NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:93062NCBITaxon:4932NEWT:644223NEWT:235443NEWT:108458NEWT:272623NEWT:272624NEWT:320637NEWT:983964NEWT:118499NEWT:32644NEWT:527796NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:476272NEWT:3747NEWT:195051NEWT:3988NEWT:1255228NEWT:649908NEWT:410289NEWT:373153NEWT:352472NEWT:1071661NEWT:360094NEWT:470NEWT:41364NEWT:1313NEWT:39491NCBITaxon:5811NEWT:29491NEWT:2014887NEWT:33952NEWT:261756NEWT:571256NEWT:40483NEWT:272634NEWT:9031NEWT:1872122NEWT:7091NEWT:141262NEWT:108931NEWT:1055524NEWT:4087NEWT:9778NEWT:306NEWT:150475NEWT:303NEWT:267872NEWT:7111NEWT:347515NEWT:9534NEWT:5180NEWT:256737NEWT:4090NEWT:4092NEWT:9541NEWT:4093NEWT:4096NEWT:185579NEWT:941442NEWT:13076NEWT:7108NEWT:40479NEWT:4076NEWT:317NEWT:1006581NEWT:885318NEWT:39488NEWT:550NEWT:4081NEWT:273068NEWT:554NEWT:98334NEWT:451516NEWT:36185NEWT:1225786NEWT:7574NEWT:1715256NEWT:30640NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:114155NEWT:6494NEWT:6491NEWT:507601NEWT:643680NEWT:214092NCBITaxon:6157NEWT:6239NEWT:162425NEWT:216257NEWT:102169NEWT:9986NEWT:4054NEWT:1268063NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:28104NEWT:407821NCBITaxon:2NEWT:985076NEWT:1215323NEWT:986NEWT:52641NEWT:7159NEWT:28532NEWT:353152NEWT:8496NEWT:27448NEWT:1194669NEWT:243230NEWT:1080772NEWT:519NEWT:8479NEWT:8485NEWT:6063NEWT:49240NEWT:6289NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:725NEWT:469008NEWT:256318NEWT:9321NEWT:206411NCBITaxon:6191NEWT:596153NEWT:229533NEWT:1836NEWT:80863NEWT:105231NEWT:52638NEWT:57075NEWT:4097NEWT:884204NEWT:9785NEWT:6279NEWT:1123869NEWT:9544NEWT:7370NEWT:83906NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:1134506NEWT:38783NEWT:4006NEWT:6426NCBITaxon:5476NEWT:6669NEWT:9935NEWT:89453NEWT:287889NEWT:51515NEWT:588596NEWT:51511NEWT:8845NEWT:92867NEWT:92866NEWT:5334NEWT:51750NEWT:202950NEWT:382352NEWT:413071NEWT:632957NEWT:9925NEWT:89462NEWT:8839NEWT:90690NEWT:4232NEWT:2758385NEWT:11298NEWT:171101NEWT:714NEWT:421932NEWT:312017NEWT:196627NEWT:200308NEWT:200302NEWT:870435NEWT:284593NEWT:100392NEWT:5315NEWT:9913NEWT:265872NEWT:105841NEWT:337330NEWT:2666255NEWT:2285NEWT:999810NCBITaxon:5693NEWT:1392998NEWT:380394NEWT:981087NEWT:180066NEWT:226900NEWT:231490NEWT:430615NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1416333NEWT:1678078NEWT:749906NEWT:418985NEWT:1817808NEWT:749907NEWT:145263NEWT:694569NEWT:27606NEWT:9739NEWT:59202NEWT:9975NEWT:51953NEWT:3197NEWT:860688NEWT:13443NEWT:36329NEWT:62615NCBITaxon:3044782NEWT:160235NEWT:72407NEWT:349741NEWT:157295NEWT:2096NEWT:192222NEWT:9721NEWT:137221NEWT:9963NEWT:1617910NEWT:565050NEWT:2785785NEWT:411901NEWT:105884NCBITaxon:9615NEWT:58334NEWT:1193501NEWT:58331NEWT:6689NEWT:298315NEWT:70448NEWT:698936NEWT:9940NEWT:3168NEWT:521001NEWT:192011NEWT:1813887NEWT:118702NEWT:118700NEWT:224911NEWT:118705NCBITaxon:11552NEWT:6945NEWT:184922NEWT:6703NEWT:2104NEWT:2NEWT:157546NEWT:474186NEWT:38942NEWT:307972NEWT:1496NEWT:2102NEWT:45474NEWT:1590841NEWT:4513NEWT:1247NEWT:6938NCBITaxon:10407NEWT:1736309NEWT:4751NEWT:10360NEWT:47664NEWT:317548NEWT:15819NEWT:3654NEWT:237561NEWT:5833NEWT:6928NEWT:1239NEWT:36745NEWT:272563NEWT:82688NEWT:3885NEWT:3888NEWT:5821NEWT:44139NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:28909NEWT:515849NEWT:3641NEWT:466585NEWT:1000589NEWT:85963NEWT:85962NEWT:143361NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:1299NEWT:34305NCBITaxon:183674NEWT:167387NEWT:84645NEWT:44586NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:431241NEWT:4550NEWT:1000561NEWT:2133NEWT:4555NEWT:294381NEWT:197NEWT:1390363NEWT:35889NEWT:288705NEWT:29176NEWT:4787NEWT:3217NEWT:3218NEWT:556182NEWT:1270NEWT:374990NEWT:1432059NEWT:156471NEWT:4784NEWT:360106NEWT:156476NEWT:4543NEWT:4785NEWT:360104NEWT:10117NEWT:10116NEWT:1280NEWT:10358NEWT:1735272NEWT:83334NEWT:83332NEWT:317513NEWT:580240NEWT:294128NEWT:11676NEWT:100226NEWT:4530NEWT:75058NEWT:13616NEWT:296543NEWT:173NEWT:316435NEWT:42157NEWT:1895NEWT:1034304NEWT:105023NEWT:866628NEWT:64152NEWT:4924NEWT:749200NEWT:9378NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:230741NCBITaxon:1313NEWT:44544NEWT:4911NEWT:645463NEWT:129249NEWT:1077286NEWT:943129NEWT:990119NEWT:77216NEWT:1111708NEWT:227321NEWT:493760NEWT:106590NEWT:260710NEWT:257313NEWT:3827NEWT:400772NEWT:128161NEWT:930945NEWT:106592NEWT:46704NEWT:1187947NEWT:269796NEWT:10312NCBITaxon:1773NEWT:54571NEWT:9598NEWT:8030NEWT:1423772NEWT:1639NEWT:188229NEWT:3818NEWT:4909NEWT:759272NEWT:432359NEWT:1182263NEWT:943146NEWT:2711NEWT:537457NEWT:9103NEWT:29159NEWT:10306NEWT:8022NEWT:145943NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:29158NEWT:3633NEWT:3635NEWT:5811NEWT:180923NEWT:1328881NEWT:411483NEWT:76336NEWT:884019NEWT:198215NEWT:411490NEWT:200298NEWT:169963NEWT:225117NEWT:746128NEWT:43665NEWT:1266464NEWT:367830NEWT:178616NEWT:375451NEWT:357NEWT:9172NEWT:411469NEWT:84023NEWT:1679NEWT:559292NEWT:411464NEWT:411460NEWT:2762NEWT:1174673NEWT:1328426NEWT:562NEWT:411470NEWT:2094720NCBITaxon:2697049NCBITaxon:138NEWT:28038NEWT:1663NEWT:62141NEWT:1423NEWT:4932NEWT:3603NEWT:2759NEWT:3847NEWT:38293NEWT:1428NEWT:327159NEWT:178876NEWT:1660NEWT:327160NEWT:573NEWT:571NEWT:241368NEWT:42528NEWT:2664373NEWT:190802NEWT:9417NEWT:1216979NEWT:7237NEWT:115104NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:220668NEWT:1821314NEWT:1249668NEWT:426430NEWT:9407NEWT:9646NEWT:5286NEWT:7227NEWT:7469NEWT:9402NEWT:9644NEWT:415540NEWT:675060NEWT:334542NEWT:313625NEWT:27592NEWT:426428NEWT:63459NEWT:588858NEWT:9638NEWT:9639NCBITaxon:11071NEWT:242231NEWT:197221NEWT:7215NEWT:2172103NCBITaxon:59201NEWT:7460NEWT:434271NEWT:747679NEWT:66712NEWT:42752NEWT:746360NEWT:89764NEWT:470150NEWT:543734NEWT:73239NEWT:38018NEWT:226186NEWT:9870NEWT:8782NEWT:115357NEWT:435590NEWT:1902NEWT:1908NEWT:13164NEWT:216129NEWT:2664179NEWT:623NEWT:7038NEWT:6192NCBITaxon:38727NEWT:2829NEWT:366581NEWT:216599NEWT:216595NEWT:40559NEWT:9689NEWT:8355NEWT:633NEWT:9685NEWT:7029NEWT:1283300NEWT:9694NEWT:186990NEWT:632NEWT:6183NEWT:630NEWT:334747NEWT:61235NEWT:343691NEWT:15368NEWT:1491466NEWT:436486NEWT:51655NEWT:170187NEWT:260707NEWT:8595NEWT:185431NEWT:29760NEWT:260704NEWT:1719NEWT:703612NEWT:260705NEWT:692672NEWT:2697049NEWT:1216981NEWT:7004NEWT:9669NCBITaxon:6073NEWT:1089448NEWT:1415167NEWT:1329349NEWT:607699NEWT:208963NEWT:208964NEWT:27337NEWT:575584NEWT:3039NEWT:8727NEWT:8726NEWT:10090NEWT:4120NEWT:5693NEWT:2186NEWT:8724NEWT:8723NEWT:283166NEWT:37334NCBITaxon:10359NEWT:1203258NEWT:242619NEWT:49317NEWT:160791NEWT:34073NEWT:373995NEWT:544404NEWT:37349NEWT:4113NEWT:837NEWT:36015NEWT:81866NEWT:5691NEWT:48479NEWT:61435NEWT:627025NEWT:1097677NEWT:375939NEWT:61674NEWT:1117957NEWT:6526NEWT:8709NEWT:4100NEWT:1159556NEWT:5671NEWT:1076NEWT:4102NEWT:4101NEWT:6763NEWT:803NEWT:29722NEWT:1692259NEWT:1486917NEWT:468911NEWT:7604NEWT:818NEWT:135588NEWT:1843183NEWT:5661NEWT:51866NEWT:29719NEWT:58002NEWT:5665NEWT:6997NEWT:452589NEWT:4577NEWT:5664NEWT:2157NEWT:146479NEWT:1042986NEWT:1911079NEWT:69373NEWT:206189NEWT:264203NEWT:1480154NEWT:1274414NEWT:9612NEWT:38626NEWT:38865NEWT:3077NEWT:9615NEWT:10299NEWT:112273NEWT:1147787NEWT:1440772NEWT:1355477NEWT:9605NEWT:9606NEWT:90371NEWT:641501NEWT:4153NEWT:278839NEWT:7668NEWT:915099NEWT:74940NEWT:13551NEWT:10041NEWT:9600NEWT:1450511NEWT:1143193NEWT:174934NEWT:179392NEWT:2823525NEWT:9838NEWT:9839NEWT:3055NEWT:11167NEWT:4146NEWT:5478NEWT:6326NEWT:5476NEWT:1450520NEWT:1042311NEWT:299767NEWT:28835NEWT:1274432NEWT:2053667NEWT:1274426NEWT:9825NEWT:1274423NEWT:393765NEWT:39946NEWT:9823NEWT:95602NEWT:8732NEWT:39947NEWT:1274420NEWT:5784580000-0002-3175-0205