Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37813_AS_L1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37821_AS_L5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37824_AS_L6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37816_AS_L2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37817_AS_L3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37814_AS_L1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37822_AS_L5.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37823_AS_L6.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37815_AS_L2.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37819_AS_L4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37820_AS_L4.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/220930_37818_AS_L3.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/552_220930_37813_AS_L1.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/561_220930_37822_AS_L5.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/559_220930_37820_AS_L4.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/557_220930_37818_AS_L3.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/553_220930_37814_AS_L1.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/560_220930_37821_AS_L5.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/556_220930_37817_AS_L3.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/562_220930_37823_AS_L6.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/555_220930_37816_AS_L2.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/558_220930_37819_AS_L4.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/563_220930_37824_AS_L6.pep.xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776/554_220930_37815_AS_L2.pep.xmlprimaryOK200acanderson@bwh.harvard.eduYuyang HanMass SpectrometryBottom-up proteomicsNot availableTgfβ1Cns autoimmunityCyp11a1HomeostasisTh17Glucocorticoidhttps://www.ebi.ac.uk/pride/archive/projects/PXD063776T CellSample protocol Phosphopeptide sample preparation Cell pellets were lysed in urea lysis buffer consisting of 9 M urea, 20 mM HEPES pH 8.0, 1 mM sodium orthovanadate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate (reagents from Cell Signaling Technology). The lysates were sonicated at 15 W output for three bursts of 30 s with 1 min rest on ice in between bursts. Then the samples were centrifuged at 20,000xg for 15 min and the protein concentration was assayed in the supernatants using a Pierce 660 nm protein assay. Five hundred micrograms of protein were digested from each sample. Disulfides were reduced with 4 mM DTT at room temperature for 1h and then alkylated with 10 mM IAA for 15 min in the dark. LysC was added at 1:200 (enzyme: substrate) for 2 h at 37 °C, then samples were diluted 4x with 20 mM HEPES pH 8. Samples were digested overnight at room temp with TPCK-treated trypsin at 1:37(enzyme: substrate). After digestion, the samples were acidified with 1% TFA and purified by solid phase extraction on 50 mg C18 Sep Paks. The eluates were dried in a speedvac. Phosphopeptides were enriched using IMAC iron (III)-NTA cartridges (Agilent) with the Agilent Bravo liquid handler following the manufacturer’s protocol. IMAC control peptides (Cell Signaling Technology cat #59524) were spiked into each sample to monitor enrichment reproducibility. Enriched peptides were eluted in 50% acetonitrile (ACN)/ 2.5% NH3, dried, then desalted on C18 tips. TMT labeling, fractionation and LCMS was carried out in a similar manner as previously described 65. Enriched peptides were resuspended in 20uL of 200mM HEPES pH 8.0 and reacted with 100ug of TMTpro 18-plex labels (Thermo Fisher Scientific) in 10uL acetonitrile (ACN). The pooled, labeled sample was desalted on a 50mg C18 Sep-Pak, then fractionated into six fractions using basic reverse-phase HPLC. LCMS analysis One fifth of each peptide fraction was injected on-column in duplicate for LCMS analysis. The LC used a 100 um x 50 cm column packed with 1.8 um C18 beads. Flowing at 280 nL/min, the analytical gradient ramped from 5 to 41% ACN in 150 min with a total run time of 180 min. Peptides were electrosprayed into a Thermo Fusion Lumos Orbitrap mass spectrometer using a MultiNotch SPS-MS3 approach as described previously 66. MS1 scans were collected with Orbitrap (OT) resolving power at 120K, scanning from 350-1400 m/z. Charge states from 2-5 were allowed and dynamic exclusion was set to 120s. The maximum cycle time for data-dependent acquisition was set at 3s. Precursors were isolated with a 0.7 m/z window prior to CID fragmentation at 35% NCE and MS2 detection in the ion trap. The trap scan rate was set to “Turbo” and the AGC target was 200%. MS3 scans also used a 0.7 m/z isolation window followed by HCD fragmentation at 65% NCE. Fragments were detected in the OT at 30K resolving power while scanning from 100-1000m/z, using a maximum injection time of 54ms and an AGC target of 500%.PrideMS data analysis MS raw files were searched against the Swissprot mouse database downloaded 25 May 2022 using the Comet search algorithm 67 implemented in GFY Core software from Harvard. Peptides were required to have at least one tryptic end (cleavage at K or R but not KP or RP) with up to five missed cleavages allowed. The precursor ion tolerance was 20ppm and the fragment mass tolerance was 1.005 Da for the ion trap MS2 spectra and 0.02 Da for OT collected MS3 spectra. Carbamidomethylcysteine and TMT on Lys and the peptide N-termini were static modifications, while oxidized Met was a variable modification. Both peptide and protein identifications were controlled at a 1% FDR cutoff using the target-decoy search approach. Phosphopeptide quantification was calculated from TMT reporter ion signal:noise (S/N) ratios summed across all peptides in each protein. Corrections were applied for isotopic impurities in the TMT reagents as indicated by the manufacturer. Only peptides that had summed S/N ≥180 across all channels and had ≥50% isolation specificity in both the MS2 and MS3 spectra were considered for quantitation. Log-median normalization was applied across all channels to reflect equal sample inputs. Log2-fold changes were used to compare the treatment groups.ProteomicsAna Carrizosa AndersonPARTIALThe Gene Lay Institute of Immunology and Inflammation Harvard Medical School and Brigham and Women's HospitalMus Musculus (mouse)10.1016/J.IMMUNI.2026.05.015yhan8@bwh.harvard.eduBrigham and Women's HospitalUnited Statesfalse A cell-intrinsic glucocorticoid biosynthesis and sensing circuit maintains homeostatic Th17 cell stateT helper 17 (Th17) cells are heterogeneous; homeostatic Th17 (Th17Hom) cells maintain tissue homeostasis and pro-inflammatory Th17 (Th17Inf) cells drive autoimmune inflammation. IL-23 drives Th17Inf, but the signals that maintain Th17Hom remain unclear. Here, we found that differential glucocorticoid (GC) production downstream of CYP11A1, a critical enzyme for steroidogenesis, distinguished Th17Hom cells from Th17Inf cells. Although TCR signaling opened the Cyp11a1 locus, TGFβ1 and IL-6, key cytokines for Th17Hom cell differentiation, maintained and amplified Cyp11a1. Th17Hom cell-derived GC signaled through the glucocorticoid receptor (GR), which was higher in Th17Hom cells compared to Th17Inf cells, thereby forming a circuit that maintained the homeostatic state. Integration of multi-omics data from CYP11A1- and GR-deficient Th17Hom cells revealed a gene network underlying this circuit. TGFβ1, a key node in the network, restored GC sensing to Th17Inf. We thus identify a GC signaling circuit that maintains Th17 homeostasis with implications for treating Th17-mediated autoimmunity.2026-06-122025-05-08PXD063776NEWT:3555NEWT:71647NEWT:35554NEWT:32046NEWT:544496NEWT:2042546NEWT:32049NEWT:1392696NEWT:259447NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:5722NCBITaxon:1280NEWT:1129NEWT:309807NEWT:55153NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:10036NEWT:1590NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:1147161NEWT:224326NEWT:1333499NCBITaxon:79857NEWT:1096976NEWT:95648NEWT:1589NEWT:135622NEWT:1349NEWT:96731NEWT:383379NEWT:10029NEWT:913645NEWT:44491NEWT:556484NEWT:317447NEWT:4688NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:3112NEWT:4442NEWT:192875NEWT:1214915NEWT:12637NEWT:59729NEWT:2164133NEWT:108061NEWT:1316931NEWT:224308NEWT:3347NEWT:511145NEWT:931281NEWT:658457NEWT:1310161NEWT:77133NEWT:295358NEWT:145481NCBITaxon:79824NCBITaxon:4563NEWT:5755NEWT:44689NEWT:44447NEWT:5759NEWT:1736231NEWT:1392NEWT:498217NEWT:2242NEWT:11320NEWT:286NEWT:391619NEWT:246196NEWT:287NEWT:246197NEWT:633149NEWT:10239NEWT:44685NEWT:161934NEWT:1148NEWT:5508NEWT:5507NEWT:410661NEWT:55571NEWT:35500NEWT:1140NCBITaxon:2157NEWT:1143NEWT:4896NEWT:1390NEWT:11557NEWT:1094343NEWT:1336795NEWT:644042NEWT:294NEWT:1773NEWT:1182590NEWT:3712NEWT:82380NEWT:935293NEWT:375146NEWT:118698NEWT:263NEWT:118696NEWT:52283NEWT:284812NEWT:8175NEWT:43330NEWT:1603293NEWT:408169NEWT:44664NEWT:47946NEWT:3702NEWT:243277NEWT:7067NEWT:118694NEWT:2850NEWT:118691NEWT:33548NEWT:274NEWT:408172NEWT:408170NEWT:3708NEWT:332648NEWT:44670NEWT:536231NEWT:376219NEWT:1436733NEWT:460519NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:9483NEWT:1513458NCBITaxon:40559NEWT:84588NEWT:1679718NEWT:480NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:300852NEWT:1502NEWT:376686NEWT:95486NEWT:508771NEWT:1883446NEWT:253NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:93062NCBITaxon:4932NEWT:644223NEWT:235443NEWT:108458NEWT:272623NEWT:272624NEWT:320637NEWT:983964NEWT:118499NEWT:32644NEWT:527796NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:476272NEWT:3747NEWT:195051NEWT:3988NEWT:1255228NEWT:649908NEWT:410289NEWT:373153NEWT:352472NEWT:1071661NEWT:360094NEWT:470NEWT:41364NEWT:1313NEWT:39491NCBITaxon:5811NEWT:29491NEWT:2014887NEWT:33952NEWT:261756NEWT:571256NEWT:40483NEWT:272634NEWT:9031NEWT:1872122NEWT:7091NEWT:141262NEWT:108931NEWT:1055524NEWT:4087NEWT:9778NEWT:306NEWT:150475NEWT:303NEWT:267872NEWT:7111NEWT:347515NEWT:9534NEWT:5180NEWT:256737NEWT:4090NEWT:4092NEWT:9541NEWT:4093NEWT:4096NEWT:185579NEWT:941442NEWT:13076NEWT:7108NEWT:40479NEWT:4076NEWT:317NEWT:1006581NEWT:885318NEWT:39488NEWT:550NEWT:4081NEWT:273068NEWT:554NEWT:98334NEWT:451516NEWT:36185NEWT:1225786NEWT:7574NEWT:1715256NEWT:30640NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:114155NEWT:6494NEWT:6491NEWT:507601NEWT:643680NEWT:214092NCBITaxon:6157NEWT:6239NEWT:162425NEWT:216257NEWT:102169NEWT:9986NEWT:4054NEWT:1268063NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:28104NEWT:407821NCBITaxon:2NEWT:985076NEWT:1215323NEWT:986NEWT:52641NEWT:7159NEWT:28532NEWT:353152NEWT:8496NEWT:27448NEWT:1194669NEWT:51329NEWT:243230NEWT:1080772NEWT:519NEWT:8479NEWT:8485NEWT:6063NEWT:49240NEWT:104395NEWT:6289NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:725NEWT:469008NEWT:256318NEWT:9321NEWT:206411NCBITaxon:6191NEWT:596153NEWT:229533NEWT:1836NEWT:80863NEWT:105231NEWT:52638NEWT:57075NEWT:4097NEWT:884204NEWT:9785NEWT:6279NEWT:1123869NEWT:9544NEWT:7370NEWT:83906NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:1134506NEWT:38783NEWT:4006NEWT:6426NCBITaxon:5476NEWT:6669NEWT:9935NEWT:89453NEWT:287889NEWT:51515NEWT:588596NEWT:51511NEWT:8845NEWT:92867NEWT:92866NEWT:5334NEWT:51750NEWT:202950NEWT:382352NEWT:413071NEWT:632957NEWT:9925NEWT:89462NEWT:8839NEWT:90690NEWT:4232NEWT:2758385NEWT:11298NEWT:171101NEWT:714NEWT:421932NEWT:312017NEWT:196627NEWT:200308NEWT:200302NEWT:870435NEWT:284593NEWT:100392NEWT:5315NEWT:9913NEWT:265872NEWT:105841NEWT:337330NEWT:2666255NEWT:2285NEWT:999810NCBITaxon:5693NEWT:1392998NEWT:380394NEWT:981087NEWT:180066NEWT:226900NEWT:231490NEWT:430615NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1416333NEWT:1678078NEWT:749906NEWT:418985NEWT:1817808NEWT:749907NEWT:145263NEWT:694569NEWT:27606NEWT:9739NEWT:59202NEWT:9975NEWT:51953NEWT:3197NEWT:860688NEWT:13443NEWT:36329NEWT:62615NCBITaxon:3044782NEWT:160235NEWT:72407NEWT:349741NEWT:157295NEWT:2096NEWT:192222NEWT:9721NEWT:137221NEWT:9963NEWT:1617910NEWT:565050NEWT:2785785NEWT:411901NEWT:105884NCBITaxon:9615NEWT:58334NEWT:1193501NEWT:58331NEWT:6689NEWT:298315NEWT:70448NEWT:698936NEWT:9940NEWT:3168NEWT:521001NEWT:192011NEWT:1813887NEWT:118702NEWT:118700NEWT:224911NEWT:118705NCBITaxon:11552NEWT:6945NEWT:184922NEWT:6703NEWT:2104NEWT:2NEWT:157546NEWT:474186NEWT:38942NEWT:307972NEWT:1496NEWT:2102NEWT:45474NEWT:1590841NEWT:4513NEWT:1247NEWT:6938NCBITaxon:10407NEWT:1736309NEWT:4751NEWT:10360NEWT:47664NEWT:317548NEWT:15819NEWT:3654NEWT:237561NEWT:5833NEWT:6928NEWT:1239NEWT:36745NEWT:272563NEWT:82688NEWT:3885NEWT:3888NEWT:5821NEWT:44139NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:28909NEWT:515849NEWT:3641NEWT:466585NEWT:1000589NEWT:85963NEWT:85962NEWT:143361NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:1299NEWT:34305NCBITaxon:183674NEWT:167387NEWT:84645NEWT:44586NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:431241NEWT:4550NEWT:1000561NEWT:2133NEWT:4555NEWT:294381NEWT:197NEWT:1390363NEWT:35889NEWT:288705NEWT:29176NEWT:4787NEWT:3217NEWT:3218NEWT:556182NEWT:1270NEWT:374990NEWT:1432059NEWT:156471NEWT:4784NEWT:360106NEWT:156476NEWT:4543NEWT:4785NEWT:360104NEWT:10117NEWT:10116NEWT:1280NEWT:10358NEWT:1735272NEWT:83334NEWT:83332NEWT:317513NEWT:580240NEWT:294128NEWT:11676NEWT:100226NEWT:4530NEWT:75058NEWT:13616NEWT:296543NEWT:173NEWT:316435NEWT:42157NEWT:1895NEWT:1034304NEWT:105023NEWT:866628NEWT:64152NEWT:4924NEWT:749200NEWT:9378NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:230741NCBITaxon:1313NEWT:44544NEWT:4911NEWT:645463NEWT:129249NEWT:1077286NEWT:943129NEWT:990119NEWT:77216NEWT:1111708NEWT:227321NEWT:493760NEWT:106590NEWT:260710NEWT:257313NEWT:3827NEWT:400772NEWT:128161NEWT:930945NEWT:106592NEWT:46704NEWT:1187947NEWT:269796NEWT:10312NCBITaxon:1773NEWT:54571NEWT:9598NEWT:8030NEWT:1423772NEWT:1639NEWT:188229NEWT:3818NEWT:4909NEWT:759272NEWT:432359NEWT:1182263NEWT:943146NEWT:2711NEWT:537457NEWT:9103NEWT:29159NEWT:10306NEWT:8022NEWT:145943NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:29158NEWT:3633NEWT:3635NEWT:5811NEWT:180923NEWT:1328881NEWT:411483NEWT:76336NEWT:884019NEWT:198215NEWT:411490NEWT:200298NEWT:169963NEWT:225117NEWT:746128NEWT:43665NEWT:1266464NEWT:367830NEWT:178616NEWT:375451NEWT:357NEWT:9172NEWT:106178NEWT:411469NEWT:84023NEWT:1679NEWT:559292NEWT:411464NEWT:411460NEWT:2762NEWT:1174673NEWT:1328426NEWT:562NEWT:411470NEWT:2094720NCBITaxon:2697049NCBITaxon:138NEWT:28038NEWT:1663NEWT:62141NEWT:1423NEWT:4932NEWT:3603NEWT:2759NEWT:3847NEWT:38293NEWT:1428NEWT:327159NEWT:178876NEWT:1660NEWT:327160NEWT:573NEWT:571NEWT:241368NEWT:42528NEWT:2664373NEWT:190802NEWT:9417NEWT:1216979NEWT:7237NEWT:115104NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:220668NEWT:1821314NEWT:1249668NEWT:426430NEWT:9407NEWT:9646NEWT:5286NEWT:7227NEWT:7469NEWT:9402NEWT:9644NEWT:415540NEWT:675060NEWT:334542NEWT:313625NEWT:27592NEWT:426428NEWT:63459NEWT:1276815NEWT:588858NEWT:9638NEWT:9639NCBITaxon:11071NEWT:242231NEWT:197221NEWT:7215NEWT:2172103NCBITaxon:59201NEWT:7460NEWT:434271NEWT:747679NEWT:66712NEWT:42752NEWT:746360NEWT:89764NEWT:470150NEWT:543734NEWT:73239NEWT:38018NEWT:226186NEWT:9870NEWT:8782NEWT:115357NEWT:435590NEWT:1902NEWT:1908NEWT:13164NEWT:216129NEWT:2664179NEWT:623NEWT:7038NEWT:6192NCBITaxon:38727NEWT:2829NEWT:366581NEWT:216599NEWT:216595NEWT:40559NEWT:9689NEWT:8355NEWT:633NEWT:9685NEWT:7029NEWT:1283300NEWT:9694NEWT:186990NEWT:632NEWT:6183NEWT:630NEWT:334747NEWT:61235NEWT:343691NEWT:15368NEWT:1491466NEWT:436486NEWT:51655NEWT:170187NEWT:260707NEWT:8595NEWT:185431NEWT:29760NEWT:260704NEWT:1719NEWT:703612NEWT:260705NEWT:692672NEWT:2697049NEWT:1216981NEWT:7004NEWT:9669NCBITaxon:6073NEWT:1089448NEWT:1415167NEWT:1329349NEWT:607699NEWT:208963NEWT:208964NEWT:27337NEWT:575584NEWT:3039NEWT:8727NEWT:8726NEWT:10090NEWT:4120NEWT:5693NEWT:2186NEWT:8724NEWT:8723NEWT:283166NEWT:37334NCBITaxon:10359NEWT:1203258NEWT:242619NEWT:49317NEWT:160791NEWT:34073NEWT:373995NEWT:5689NEWT:544404NEWT:37349NEWT:4113NEWT:837NEWT:36015NEWT:81866NEWT:5691NEWT:48479NEWT:61435NEWT:627025NEWT:1097677NEWT:375939NEWT:61674NEWT:1117957NEWT:6526NEWT:8709NEWT:4100NEWT:1159556NEWT:5671NEWT:1076NEWT:4102NEWT:4101NEWT:6763NEWT:803NEWT:29722NEWT:1692259NEWT:1486917NEWT:419947NEWT:468911NEWT:817NEWT:7604NEWT:818NEWT:135588NEWT:1843183NEWT:5661NEWT:51866NEWT:29719NEWT:58002NEWT:5665NEWT:6997NEWT:452589NEWT:4577NEWT:5664NEWT:2157NEWT:146479NEWT:1042986NEWT:1911079NEWT:69373NEWT:206189NEWT:264203NEWT:1480154NEWT:1274414NEWT:9612NEWT:38626NEWT:38865NEWT:3077NEWT:9615NEWT:10299NEWT:112273NEWT:1147787NEWT:1440772NEWT:1355477NEWT:9605NEWT:9606NEWT:90371NEWT:641501NEWT:4153NEWT:278839NEWT:7668NEWT:915099NEWT:74940NEWT:13551NEWT:10041NEWT:9600NEWT:1450511NEWT:1143193NEWT:174934NEWT:179392NEWT:2823525NEWT:9838NEWT:9839NEWT:3055NEWT:11167NEWT:4146NEWT:5478NEWT:6326NEWT:5476NEWT:1450520NEWT:1042311NEWT:299767NEWT:28835NEWT:1274432NEWT:2053667NEWT:1274426NEWT:9825NEWT:1274423NEWT:393765NEWT:39946NEWT:9823NEWT:95602NEWT:8732NEWT:314539NEWT:39947NEWT:718274NEWT:1274420NEWT:578458