Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063853/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063853/Results.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063853/raw.zipprimaryOK2001419974394@qq.comXinyue LiData-dependent acquisitionMass SpectrometryLc-msIphttps://www.ebi.ac.uk/pride/archive/projects/PXD063853Stem CellCell CultureThe samples were treated with RAP kit and get the RAP-proteins. The protein samples were removed from-80℃ and melted on ice. An appropriate amount of TEAB was added to adjust pH 8.0. 5 μ L of suspension was used for SDS-PAGE testing；For digestion, the protein solution was reduced with 5 mM dithiothreitol for 30 min at 56 °C and alkylated with 11mM iodoacetamide for 15 min at room temperature in darkness. The protein sample was then diluted by adding 100mM TEAB to urea concentration less than 2 M. Finally, trypsin was added at 1:50 trypsin-to-protein mass ratio for the first digestion overnight and 1:100 trypsin-to-protein mass ratio for a second 4 h-digestion. Finally, the peptides were desalted by C18 SPE column.PrideNot availableThe DIA data were processed using Spectronaut (v.18) software.Tandem mass spectra were searched against the Mus musculus 10090 SP _ _ _ _ 20231220.fasta (17191 entries) concatenated with reverse decoy database.Trypsin/P was specified as cleavage enzyme allowing up to 2 missing cleavages. Carbamidomethyl on Cys was specified as fixed modification. Acetylation on protein N-terminal, and oxidation on Met were specified as variable modifications. False discovery rate (FDR) of protein, peptide and PSM was adjusted to < 1%. we used PSORTb software to perform subcellular structure prediction analysis of the above four classes of proteins identified in prokaryotes. Fisher’s exact test was used to analyze the significance of functional enrichment of differentially expressed proteins (using the identified protein as the background).ProteomicsXinyue LiPARTIALshanghai jiaotong universityMus Musculus (mouse)41841563 Li X, Hu X, Wu J. N4-acetylcytidine in LncRNA Gm26917 Promotes Translation in Female Germline Stem Cells by Recruiting Ribosomal Protein mRNA via EEF1A1. Adv Sci (Weinh). 2026 13(30):e20059 10.1002/advs.2025200591419974394@qq.comshanghai jiaotong universityChinaN4-acetylcytidine (ac4C) modification, present in both long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs), regulates cell fate decisions, yet its role in female germline stem cell (FGSC) development remains poorly understood. Here, we demonstrate that ac4C modification depletion (in vitro or in vivo) impairs FGSC maintenance by disrupting the EEF1A1-mediated Gm26917-Rpl10 interaction, leading to attenuated protein synthesis. Reduced ac4C levels suppress FGSC viability and proliferation, dysregulate cell cycle progression, and promote differentiation and apoptosis. Integrated acRIP-seq and RNA in situ conformation sequencing (RIC-seq) analyses demonstrate that diminished ac4C modification in lncRNA Gm26917 compromises its stability and weakens its interaction with Rpl10. Strikingly, the reduction of RPL10 is primarily caused by impaired Gm26917-Rpl10 interaction rather than direct ac4C modification. Gm26917 or Rpl10 knockdown disrupts FGSC fate, while ribosome profiling sequencing (Ribo-seq) reveals that Gm26917 depletion significantly reduces mRNA translation efficiency (TE), a defect rescued by Rpl10 overexpression. Furthermore, EEF1A1 disruption diminishes the Gm26917-Rpl10 interaction and decreases mRNA TE. This work establishes that ac4C modification on lncRNAs governs their spatial interactions with mRNAs, elucidates a novel ac4C-Gm26917-EEF1A1-Rpl10 axis in FGSC maintenance, and provides a potential molecular target for modulating germ cell development and fertility.N4-acetylcytidine in LncRNA Gm26917 Promotes Translation in Female Germline Stem Cells by Recruiting Ribosomal Protein mRNA via EEF1A1.Li Xinyue X, Hu Xiaopeng X, Wu Ji Jfalse4DSmartDIA protein quantification omics of two mouse IP elution samples (no duplication)Based on the Raw files obtained by mass spectrometry detection, 1) Construct a sample-specific protein database according to the source of the samples, and then conduct database search using analysis software; 2) Conduct quality control analysis at the peptide and protein levels based on the results of database search; 3) Conduct quantitative analysis of proteins, including quantitative distribution and repeatability analysis; 4) Perform common functional annotations on the identified proteins, including GO, KEGG, Protein domain, COG/KOG, STRING database annotations, etc. 6) Functional classification statistical analysis of proteins between the two groups was conducted, including GO secondary classification, subcellular localization classification, COG/KOG classification and KEGG pathway classification statistics; 7) The Fisher's exact test method was used to conduct enrichment analysis on the differentially expressed proteins between the two groups, involving the functions of GO, KEGG and Protein domain; 8) When the project has multiple experimental groups, the functional relationships of differentially expressed proteins under different experimental conditions are compared through rich cluster analysis. 9) Through Protein-protein interaction network (PPI) analysis, the key regulatory proteins under specific experimental conditions were screened out.2026-06-152025-05-12PXD063853NEWT:6945NEWT:184922NEWT:6703NEWT:3555NEWT:71647NEWT:2NEWT:157546NEWT:474186NEWT:35554NEWT:38942NEWT:307972NEWT:32046NEWT:1496NEWT:544496NEWT:2102NEWT:2042546NEWT:259447NEWT:45351NEWT:445974NEWT:43179NEWT:4513NEWT:180454NEWT:5722NEWT:1247NEWT:6938NEWT:1129NEWT:376741NEWT:55153NCBITaxon:10407NEWT:1736309NEWT:309800NEWT:281395NEWT:10360NEWT:1211601NEWT:876138NEWT:47664NEWT:317548NEWT:3654NEWT:237561NEWT:5833NEWT:6928NEWT:10036NEWT:36745NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:272563NEWT:1147161NEWT:224326NEWT:1333499NCBITaxon:79857NEWT:1096976NEWT:82688NEWT:95648NEWT:3885NEWT:3888NEWT:5821NEWT:1589NEWT:44139NEWT:135622NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:28909NEWT:515849NEWT:3641NEWT:383379NEWT:466585NEWT:10029NEWT:913645NEWT:1000589NEWT:85963NEWT:85962NEWT:143361NEWT:317447NEWT:4688NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:398580NEWT:4442NEWT:4565NEWT:1264690NEWT:515619NEWT:192875NEWT:34305NEWT:59729NEWT:2164133NCBITaxon:183674NEWT:1316931NEWT:224308NEWT:167387NEWT:84645NEWT:44586NEWT:626528NEWT:3347NEWT:511145NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:431241NEWT:931281NEWT:4550NEWT:1000561NEWT:2133NEWT:294381NEWT:197NEWT:1390363NEWT:77133NEWT:288705NEWT:29176NEWT:145481NCBITaxon:79824NEWT:4787NCBITaxon:4563NEWT:5755NEWT:44689NEWT:3218NEWT:5759NEWT:1736231NEWT:556182NEWT:1270NEWT:374990NEWT:1392NEWT:498217NEWT:156471NEWT:2242NEWT:4784NEWT:11320NEWT:360106NEWT:156476NEWT:286NEWT:391619NEWT:360104NEWT:246196NEWT:287NEWT:246197NEWT:10117NEWT:10239NEWT:10116NEWT:1280NEWT:1735272NEWT:83334NEWT:83332NEWT:44685NEWT:317513NEWT:161934NEWT:1148NEWT:580240NEWT:5508NEWT:294128NEWT:5507NEWT:11676NEWT:55571NEWT:35500NEWT:1140NEWT:100226NEWT:4530NEWT:1143NEWT:4896NEWT:75058NEWT:13616NEWT:1390NEWT:1094343NEWT:1336795NEWT:172NEWT:644042NEWT:296543NEWT:316435NEWT:42157NEWT:1773NEWT:1895NEWT:1182590NEWT:3712NEWT:82380NEWT:1034304NEWT:105023NEWT:866628NEWT:215358NEWT:935293NEWT:64152NEWT:4924NEWT:749200NEWT:375146NEWT:9378NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:263NEWT:230741NEWT:52283NEWT:284812NEWT:8175NCBITaxon:1313NEWT:43330NEWT:1603293NEWT:408169NEWT:44544NEWT:47946NEWT:4911NEWT:645463NEWT:3702NEWT:129249NEWT:1077286NEWT:943129NEWT:243277NEWT:990119NEWT:7067NEWT:2850NEWT:1111708NEWT:408172NEWT:227321NEWT:408170NEWT:493760NEWT:106590NEWT:260710NEWT:257313NEWT:3827NEWT:400772NEWT:3708NEWT:128161NEWT:332648NEWT:930945NEWT:106592NEWT:536231NEWT:46704NEWT:1436733NEWT:460519NEWT:1187947NEWT:572307NEWT:1432138NEWT:269796NEWT:10312NEWT:1424507NCBITaxon:1773NEWT:1194599NEWT:272844NEWT:54571NEWT:9598NEWT:8030NEWT:9483NEWT:1513458NCBITaxon:40559NEWT:1639NEWT:188229NEWT:3818NEWT:480NEWT:4909NEWT:67767NEWT:759272NEWT:432359NEWT:46835NEWT:1182263NEWT:109757NEWT:943146NEWT:2711NEWT:300852NEWT:537457NEWT:1502NEWT:376686NEWT:95486NEWT:9103NEWT:508771NEWT:1883446NEWT:29159NEWT:253NEWT:10306NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:8022NEWT:145943NCBITaxon:4932NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:29158NEWT:644223NEWT:3635NEWT:5811NEWT:235443NEWT:180923NEWT:108458NEWT:272623NEWT:272624NEWT:411483NEWT:884019NEWT:198215NEWT:411490NEWT:983964NEWT:118499NEWT:169963NEWT:32644NEWT:527796NEWT:225117NEWT:746128NEWT:499175NEWT:109779NEWT:43665NEWT:1266464NEWT:1715989NCBITaxon:4751NEWT:476272NEWT:3747NEWT:195051NEWT:3988NEWT:367830NEWT:1255228NEWT:178616NEWT:649908NEWT:410289NEWT:373153NEWT:375451NEWT:352472NEWT:357NEWT:1071661NEWT:360094NEWT:470NEWT:41364NEWT:1313NEWT:411469NEWT:84023NEWT:1679NEWT:559292NEWT:39491NCBITaxon:5811NEWT:29491NEWT:411464NEWT:411460NEWT:2014887NEWT:2762NEWT:1174673NEWT:1328426NEWT:562NEWT:411470NEWT:33952NEWT:2094720NCBITaxon:2697049NEWT:571256NCBITaxon:138NEWT:40483NEWT:28038NEWT:1663NEWT:1423NEWT:4932NEWT:3603NEWT:2759NEWT:3847NEWT:38293NEWT:1428NEWT:327159NEWT:178876NEWT:1660NEWT:327160NEWT:573NEWT:9031NEWT:1872122NEWT:7091NEWT:108931NEWT:241368NEWT:1055524NEWT:42528NEWT:190802NEWT:9778NEWT:150475NEWT:303NEWT:9417NEWT:7111NEWT:347515NEWT:1216979NEWT:7237NEWT:9534NEWT:5180NEWT:256737NEWT:9541NEWT:115104NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:185579NEWT:941442NEWT:220668NEWT:13076NEWT:1821314NEWT:1249668NEWT:7108NEWT:40479NEWT:317NEWT:5286NEWT:7227NEWT:7469NEWT:885318NEWT:9402NEWT:9644NEWT:415540NEWT:550NEWT:675060NEWT:4081NEWT:334542NEWT:273068NEWT:554NEWT:27592NEWT:98334NEWT:426428NEWT:451516NEWT:63459NEWT:1276815NEWT:36185NEWT:588858NEWT:1225786NEWT:9639NCBITaxon:11071NEWT:242231NEWT:7574NEWT:1715256NEWT:197221NEWT:7215NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:2172103NEWT:6494NEWT:7460NEWT:6491NEWT:507601NEWT:434271NEWT:643680NCBITaxon:6157NEWT:42752NEWT:746360NEWT:6239NEWT:89764NEWT:470150NEWT:162425NEWT:216257NEWT:102169NEWT:543734NEWT:9986NEWT:4054NEWT:73239NEWT:38018NEWT:226186NEWT:1268063NEWT:8782NEWT:1263854NEWT:118503NEWT:2059687NEWT:115357NEWT:435590NEWT:1902NEWT:160488NEWT:28104NEWT:1908NEWT:13164NEWT:216129NCBITaxon:2NEWT:98507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