Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-59_MS1Quant_Qclassic_LocalNorm_NoImp.xlsxftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-34_MS1Quant_Qclassic_LocalNorm_NoImp.xlsxftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-34_DMSO_2b.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-59_5PhIAA_2b.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-59_DMSO_1a.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-59_DMSO_1c.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-34_5PhIAA_3c.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-34_5PhIAA_3a.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-34_DMSO_2c.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-59_5PhIAA_2a.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-59_DMSO_1b.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-59_5PhIAA_2c.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-34_DMSO_2a.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065157/2022-34_5PhIAA_3b.rawprimary200OKj.c.wolters@umcg.nlJustina Clarinda WoltersMass SpectrometryData-independent acquisitionAcute LeukemiaHumanExploris480https://www.ebi.ac.uk/pride/archive/projects/PXD065157LeukocytePull outs For pull outs nuclear extraction was carried out on 1x107 cells for small scale and 1-2x108 cells for large scale pull outs. Nuclear extract preparation was done as described previously {van den Boom, 2013 #294}. For large scale pull outs, nuclear extracts were precleared by incubating them with pre-equilibrated magnetic agarose beads (Chromotek) at 4°C on a rotating platform. Subsequently, the samples were incubated with pre-equilibrated GFP-Trap, Spot-Trap, or Myc-Trap magnetic agarose beads (Chromotek), for 1h at 4°C on a rotating platform. After incubation, beads were washed six times with wash buffer (TBS, 0.3% IGEPAL CA-630, 1x CLAP, 0.1 mM PMSF) and subsequently boiled for 10 min in 2x Laemmli sample buffer. Proteomics sample preparation Immunoprecipitated samples were loaded on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad) followed by Coomassie Blue staining (Imperial™ Protein Stain; Thermo Scientific), destaining using milliQ-H2O, and excision of the band containing all proteins. The gel band was sliced into small pieces, washed subsequently with 30% and 50% v/v acetonitrile in 100 mM ammonium bicarbonate (dissolved in milliQ-H2O), each incubated at RT for 30 min while mixing (500 rpm) and lastly with 100% acetonitrile for 5 min, before drying the gel pieces at 55°C. The proteins were reduced with 10 mM dithiothreitol (in 100 mM ammonium bicarbonate dissolved in milliQ-H2O, 30 min, 55 °C) and alkylated with 55 mM iodoacetamide (in 100 mM ammonium bicarbonate dissolved in milliQ-H20, 30 min, in the dark at RT). The gel pieces were washed with 100 mM ammonium bicarbonate for 10 minutes followed by a 100% acetonitrile wash for 30 min while mixing (500 rpm) and dried in an oven at 55°C before overnight digestion with using 5 ng/μL trypsin (sequencing grade modified trypsin V5111, Promega; diluted in 100 mM ammonium bicarbonate) at 37 °C. The next day, the peptides were first eluted from the gel pieces using 5% v/v formic acid in milliQ-H2O followed by a second elution using 75% v/v acetonitrile plus 5% v/v formic acid. The combined eluted fractions were dried under vacuum and resuspended in 25 μL 5% v/v formic acid in milliQ-H2O. Discovery-based proteomics analyses Discovery mass spectrometric analyses were performed on a quadrupole orbitrap mass spectrometer equipped with a nano-electrospray ion source (Orbitrap Exploris 480, Thermo Scientific). Chromatographic separation of the peptides was performed by liquid chromatography (LC) on a Evosep system (Evosep One, Evosep) using a nano-LC column (EV1137 Performance column 15 cm x 150 µm, 1.5 µm, Evosep; buffer A: 0.1% v/v formic acid, dissolved in milliQ-H2O, buffer B: 0.1% v/v formic acid, dissolved in acetonitrile). The digests were injected in the LC-MS with 20 µL of the peptide digests and the peptides were separated using the 30SPD workflow (Evosep). The mass spectrometer was operated in positive ion mode and data-independent acquisition mode (DIA) using isolation windows of 16 m/z with a precursor mass range of 400-1000, switching the FAIMS between CV-45V and -60V with three scheduled MS1 scans during each screening of the precursor mass range.PrideLC-MS raw data were processed with Spectronaut (version 16.0.220606) (Biognosys) with a Human SwissProt database (20376 entries) using the standard settings of the directDIA workflow except that quantification was performed on MS1. For the quantification, the Q-value filtering was set to the classic setting with local normalization and no imputation. For downstream processing LFQ values were processed using Perseus 1.5.8.5 and volcano plots were generated. The single DDA measurement added to this manuscript was processed the same as described previously: PMID 31199242ProteomicsDr. Justina Clarinda WoltersDepartment of Pediatrics, UMCG, Groningen, The NetherlandsPARTIALHomo Sapiens (human)j.c.wolters@umcg.nl42273916 Mojallali F, De Meis A, Alkema SG, Atsma TJ, Hogeling SM, Kloosterman R, Ioannou E, Wierenga ATJ, Huls G, Martens JHA, Schuringa JJ, van den Boom V. Sustained PRC1.1 activity preserves gene repression independently of PRC2.2 and restrains leukemic cell differentiation. Nucleic Acids Res. 2026 54(11):gkag529 10.1093/nar/gkag529UMCGNetherlandsLoss-of-function mutations in BCOR, a subunit of the non-canonical Polycomb Repressive Complex 1.1 (PRC1.1), are frequently observed in acute myeloid leukemia (AML) and associate with adverse risk. Paradoxically, leukemic stem cell viability in BCOR wild-type AMLs strongly depends on PRC1.1 activity. Here, we use BCOR and KDM2B degron models to study PRC1.1 dependency in leukemic cells and find that BCOR is a bridging factor tethering the catalytic and chromatin-binding moieties of the PRC1.1 complex. BCOR degradation induces a quick localized loss of H2AK119ub at PRC1.1 target genes, whereas PRC2-induced H3K27me3 remains unaffected. Degron-mediated depletion of BCOR or KDM2B induces a rapid but time-dependent transcriptional induction, whereby late-upregulated genes are more heavily decorated with H3K27me3 compared to early-upregulated genes. Combined PRC1.1 inactivation and PRC2 inhibition further amplifies gene induction, suggesting collaborative yet distinct control over target genes. Strikingly, both JARID2/AEBP2 and SUZ12 knockout cells, devoid of PRC2.2 or PRC2.1/PRC2.2 respectively, retain PRC1.1 loss-induced transcriptional activation, underscoring that PRC1.1 can repress target genes independently of a downstream PRC2.2-canonical PRC1 repressive axis. Finally, combined targeting of PRC1.1 and PRC2 induces differentiation of leukemic cells, emphasizing that co-targeting PRC1.1 and PRC2 represents a promising strategy to improve treatment of AML patients.Sustained PRC1.1 activity preserves gene repression independently of PRC2.2 and restrains leukemic cell differentiation.Mojallali Fatemeh F, De Meis Alessandra A, Alkema Sinne G SG, Atsma Tjerk Jan TJ, Hogeling Shanna M SM, Kloosterman Rianne R, Ioannou Eve E, Wierenga Albertus T J ATJ, Huls Gerwin G, Martens Joost H A JHA, Schuringa Jan Jacob JJ, van den Boom Vincent VfalseSustained PRC1.1 activity preserves gene repression independent of PRC2.2 and suppresses leukemic cell differentiationLoss-of-function mutations in BCOR, subunit of the non-canonical Polycomb Repressive Complex 1.1 (PRC1.1), are frequently observed in acute myeloid leukemia (AML) and associate with adverse risk, but underlying mechanisms driving leukemogenesis remain elusive. Here, we find that BCOR is a bridging factor tethering the catalytic and chromatin-binding moieties of the PRC1.1 complex. Degron-mediated depletion of BCOR or KDM2B induces a rapid but time-dependent transcriptional induction, whereby early-upregulated genes have a distinct epigenetic profile compared to late-upregulated genes that are more heavily decorated with H3K27me3. Combined KDM2B degradation and PRC2 inhibition further amplifies gene induction suggesting distinct yet collaborative control over target genes. Strikingly, both JARID2/AEBP2 and SUZ12 knockout cells, completely devoid of PRC2 functionality, retain PRC1.1-loss induced transcriptional activation, underscoring that PRC1.1 can repress target genes independent of a downstream PRC2.2-canonical PRC1 repressive axis. Finally, combined targeting of PRC1.1 and PRC2 induces differentiation of leukemic cells emphasizing that co-targeting PRC1.1 and PRC2 represents a promising strategy to improve treatment of AML patients2026-06-152025-06-18PXD065157NEWT:3555NEWT:71647NEWT:330879NEWT:35554NCBITaxon:12939NEWT:32046NEWT:544496NEWT:2042546NEWT:32049NEWT:1392696NEWT:259447NEWT:295546NCBITaxon:2719036NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:5722NCBITaxon:1280NEWT:1129NEWT:309807NEWT:10245NEWT:55153NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:193516NEWT:351581NEWT:43186NEWT:428146NEWT:498257NEWT:10036NEWT:1590NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:1147161NEWT:661410NEWT:638632NEWT:263737NEWT:224326NEWT:1333499NCBITaxon:79857NEWT:1096976NEWT:5702NEWT:95648NEWT:1589NEWT:135622NEWT:1349NEWT:35786NEWT:1580NEWT:399784NEWT:96731NEWT:383379NEWT:1401257NEWT:418106NEWT:10029NEWT:913645NEWT:44491NEWT:641809NEWT:27933NEWT:556484NEWT:317447NEWT:4688NEWT:7955NEWT:7719NEWT:7959NEWT:2261NEWT:868565NEWT:31156NEWT:3112NEWT:79329NEWT:4442NEWT:192875NEWT:1214915NEWT:12637NEWT:59729NEWT:2164133NEWT:295105NEWT:108061NEWT:60711NEWT:1316931NEWT:224308NEWT:3347NEWT:511145NEWT:160621NEWT:212790NEWT:931281NEWT:4432NEWT:5762NEWT:5763NEWT:658457NEWT:1310161NEWT:77133NEWT:295358NEWT:145481NCBITaxon:79824NEWT:2246NEWT:2652724NEWT:5757NCBITaxon:4563NEWT:5755NEWT:44689NEWT:44447NEWT:752555NEWT:498211NEWT:5759NEWT:347256NEWT:1736231NEWT:5518NEWT:398007NEWT:1527468NEWT:1392NEWT:498217NEWT:498216NEWT:2242NEWT:11320NEWT:286NEWT:391619NEWT:246196NEWT:287NEWT:246197NEWT:145458NEWT:633149NEWT:1149786NEWT:10239NEWT:68895NEWT:44685NEWT:161934NEWT:4897NEWT:1148NEWT:5744NEWT:5508NEWT:3329NEWT:5507NEWT:410661NEWT:55571NEWT:35500NEWT:1235816NEWT:1140NCBITaxon:2157NEWT:1143NEWT:4896NEWT:1287689NEWT:1390NEWT:11557NEWT:1094343NEWT:1462472NEWT:1336795NEWT:644042NEWT:294NEWT:1773NEWT:1182590NEWT:3712NEWT:82380NEWT:3711NEWT:935293NEWT:375146NEWT:270643NEWT:2065263NEWT:1772NEWT:118698NEWT:1616117NEWT:263NEWT:118696NEWT:52283NEWT:11988NEWT:284812NEWT:8175NEWT:400667NEWT:43330NEWT:1603293NEWT:408169NEWT:44664NEWT:47946NEWT:459758NEWT:3702NEWT:1245466NEWT:243277NEWT:1246791NEWT:7067NEWT:118694NEWT:586722NEWT:2850NEWT:118691NEWT:33548NEWT:274NEWT:7070NCBITaxon:287NEWT:408172NEWT:408170NEWT:96794NEWT:3708NEWT:332648NEWT:44670NEWT:536231NEWT:376219NEWT:219813NEWT:1510NEWT:1436733NEWT:460519NEWT:1247411NEWT:1515NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:9483NEWT:333760NEWT:56636NEWT:1513458NEWT:1233681NCBITaxon:40559NEWT:506599NEWT:2853422NEWT:81077NEWT:84588NEWT:1679718NEWT:480NEWT:65349NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:300852NEWT:1502NEWT:128017NEWT:376686NEWT:95486NEWT:508771NEWT:1883446NEWT:253NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:93062NEWT:34613NCBITaxon:4932NEWT:109760NEWT:943274NEWT:644223NEWT:235443NEWT:108458NEWT:5936NEWT:272623NEWT:272624NEWT:320637NEWT:886559NEWT:55521NEWT:983964NEWT:118499NEWT:11706NEWT:32644NEWT:527796NEWT:1130798NEWT:55529NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:476272NEWT:3747NEWT:195051NEWT:3988NEWT:1116234NEWT:1255228NEWT:649908NEWT:410289NEWT:373153NEWT:3983NEWT:203696NEWT:352472NEWT:1071661NEWT:1202532NEWT:360094NEWT:470NEWT:41364NEWT:5911NEWT:1313NEWT:1218097NCBITaxon:50557NEWT:39251NEWT:39491NEWT:212142NCBITaxon:5811NEWT:29491NEWT:101841NEWT:2014887NEWT:33952NEWT:153009NEWT:261756NEWT:571256NEWT:40483NEWT:63366NEWT:63367NEWT:688333NEWT:215402NCBITaxon:548681NEWT:318586NCBITaxon:5820NEWT:1547NEWT:272634NEWT:198107NEWT:9031NEWT:1872122NEWT:1308NEWT:7091NEWT:1034015NEWT:760568NEWT:141262NEWT:108931NEWT:1055524NEWT:4087NEWT:9778NEWT:306NEWT:150475NEWT:303NEWT:267872NEWT:97477NEWT:7111NEWT:347515NEWT:172269NEWT:9534NEWT:6269NEWT:5180NEWT:256737NEWT:4090NEWT:4092NEWT:9541NEWT:8694NEWT:4093NEWT:4096NEWT:8692NEWT:2903NEWT:185579NEWT:941442NEWT:536088NEWT:13076NEWT:1226408NEWT:7108NEWT:40479NEWT:43989NEWT:4076NEWT:142615NEWT:317NEWT:1006581NEWT:885318NEWT:39488NEWT:550NEWT:4081NEWT:273068NEWT:554NEWT:98334NEWT:451516NEWT:4084NEWT:63577NEWT:36185NEWT:1225786NEWT:7574NEWT:1715256NEWT:30640NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:114155NEWT:6494NEWT:6491NEWT:507601NEWT:520NEWT:186441NEWT:634955NEWT:643680NEWT:214092NCBITaxon:6157NEWT:6239NEWT:13095NEWT:162425NEWT:216257NEWT:102169NEWT:208893NEWT:9986NEWT:4054NEWT:8654NEWT:8658NEWT:1268063NEWT:8657NEWT:8655NEWT:5147NEWT:98351NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:28104NEWT:207559NEWT:407821NCBITaxon:2NEWT:85057NEWT:985076NEWT:568708NEWT:1215323NEWT:986NEWT:52641NEWT:7159NEWT:267671NEWT:28532NEWT:353152NEWT:8496NEWT:7165NEWT:1257118NEWT:27448NEWT:40674NEWT:1194669NEWT:51329NEWT:243230NEWT:443906NEWT:1080772NEWT:519NEWT:8479NEWT:8485NEWT:6063NEWT:49240NEWT:1328388NEWT:104395NEWT:9557NEWT:9315NEWT:6289NEWT:377586NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:9554NEWT:118562NEWT:725NEWT:7137NEWT:469008NEWT:256318NEWT:9321NEWT:206411NCBITaxon:6191NEWT:596153NEWT:229533NEWT:1836NEWT:214053NCBITaxon:10298NEWT:80863NEWT:90675NEWT:105231NEWT:104385NEWT:74649NEWT:52638NEWT:57075NEWT:206672NEWT:4097NEWT:8697NEWT:8695NEWT:884204NEWT:71139NEWT:9785NEWT:6279NEWT:1123869NEWT:9544NEWT:9545NEWT:7370NEWT:9793NEWT:83906NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:1134506NEWT:255470NEWT:27457NEWT:38783NEWT:4006NEWT:6426NCBITaxon:5476NEWT:113208NEWT:33090NEWT:6669NEWT:9935NEWT:89453NEWT:287889NEWT:1114301NEWT:51515NEWT:588596NEWT:51511NEWT:8845NEWT:92867NEWT:92866NEWT:419610NEWT:5334NEWT:51750NEWT:202950NEWT:382352NEWT:413071NCBITaxon:10239NCBITaxon:714NEWT:295027NCBITaxon:11320NEWT:632957NEWT:11051NEWT:9925NEWT:89462NCBITaxon:9606NEWT:452680NEWT:8839NEWT:90690NEWT:1436183NEWT:4232NEWT:2758385NEWT:416348NEWT:11298NEWT:171101NEWT:714NEWT:5322NEWT:421932NEWT:312017NEWT:196627NEWT:200308NEWT:553470NEWT:200302NEWT:870435NEWT:284593NEWT:100392NEWT:655819NEWT:5315NEWT:9913NEWT:265872NEWT:105841NEWT:337330NEWT:2666255NEWT:2285NEWT:2287NEWT:999810NCBITaxon:5693NEWT:28995NEWT:1392998NEWT:380394NEWT:981087NEWT:180066NEWT:226900NEWT:1266738NEWT:231490NEWT:244704NEWT:7725NEWT:430615NEWT:563041NEWT:72664NEWT:174633NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1416333NEWT:35024NEWT:135461NEWT:1678078NEWT:7962NEWT:749906NEWT:418985NEWT:1817808NEWT:749907NEWT:145262NEWT:145263NEWT:431947NEWT:69014NEWT:142809NEWT:694569NEWT:27606NEWT:9739NEWT:59202NEWT:9975NEWT:8643NEWT:51953NEWT:502780NEWT:57267NEWT:3197NEWT:860688NEWT:13443NEWT:8644NEWT:36329NEWT:62615NEWT:5141NEWT:46360NCBITaxon:3044782NEWT:1333550NEWT:160235NEWT:72407NEWT:8639NEWT:1928434NEWT:8637NEWT:349741NEWT:1114970NEWT:409859NEWT:157295NEWT:2096NEWT:509169NEWT:192222NEWT:9721NEWT:137221NEWT:9963NEWT:1617910NEWT:565050NEWT:409860NEWT:2785785NEWT:411901NEWT:8626NEWT:105884NCBITaxon:9615NEWT:8621NEWT:58334NEWT:452659NEWT:1193501NEWT:58331NEWT:6689NEWT:8624NEWT:5116NEWT:8623NEWT:5597NCBITaxon:3077NEWT:298315NEWT:8618NEWT:70448NEWT:9704NEWT:698936NEWT:1009874NEWT:8855NEWT:9940NEWT:3168NEWT:1247190NEWT:521001NEWT:192011NEWT:1813887NEWT:118702NEWT:118700NEWT:2126719NEWT:224911NEWT:118705NCBITaxon:11552NEWT:334249NEWT:6945NEWT:184922NEWT:6703NEWT:2104NEWT:6943NEWT:2NEWT:157546NEWT:35670NEWT:511693NEWT:870726NEWT:474186NEWT:38942NEWT:1493NEWT:307972NEWT:1496NEWT:2102NEWT:3674NEWT:660469NEWT:1365186NEWT:38949NEWT:45474NEWT:1299114NEWT:1590841NEWT:2282334NEWT:4513NEWT:1247NEWT:70667NEWT:869728NEWT:5843NEWT:6938NCBITaxon:39054NCBITaxon:10407NEWT:1736309NEWT:4751NEWT:10360NEWT:47664NEWT:317548NEWT:15819NEWT:3654NEWT:237561NEWT:1085956NEWT:5833NEWT:3659NEWT:6928NEWT:1239NEWT:36745NEWT:499229NEWT:31298NEWT:400727NEWT:1204162NEWT:272563NEWT:11009NEWT:82688NEWT:5823NEWT:3885NEWT:3888NEWT:5821NEWT:44139NEWT:649408NCBITaxon:4895NCBITaxon:4896NEWT:315393NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:216816NEWT:28909NEWT:515849NEWT:3641NEWT:10141NEWT:466585NEWT:1000589NEWT:85963NEWT:85962NEWT:42197NEWT:143361NCBITaxon:5796NEWT:12993NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:1299NEWT:316407NEWT:34305NCBITaxon:183674NEWT:167387NEWT:84645NEWT:44586NEWT:1123294NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:431241NEWT:4550NEWT:1000561NEWT:2133NEWT:4555NEWT:294381NEWT:197NEWT:1390363NEWT:35889NEWT:406072NEWT:144030NEWT:288705NEWT:29176NEWT:4787NEWT:3217NEWT:113549NEWT:4547NEWT:3218NEWT:556182NEWT:1270NEWT:374990NEWT:1432059NEWT:154293NEWT:156471NEWT:4784NEWT:3694NEWT:360106NEWT:156476NEWT:4543NEWT:4785NEWT:210007NEWT:360104NEWT:1857017NEWT:10117NEWT:10359NEWT:10116NEWT:1280NEWT:10358NEWT:83333NEWT:1735272NEWT:83334NEWT:83332NEWT:317513NEWT:580240NEWT:294128NEWT:11676NEWT:100226NEWT:718308NEWT:4530NEWT:176299NEWT:5861NEWT:54126NEWT:75058NEWT:13616NEWT:296543NEWT:173NEWT:316435NEWT:42157NEWT:1895NEWT:1034304NEWT:105023NEWT:866628NEWT:64152NEWT:4924NEWT:749200NEWT:2748NEWT:8049NEWT:33892NEWT:9378NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:203124NEWT:192952NEWT:230741NEWT:8296NEWT:117008NCBITaxon:1313NEWT:167748NEWT:1163394NEWT:44544NEWT:529818NEWT:400540NEWT:4911NEWT:645463NEWT:152480NEWT:129249NEWT:1077286NEWT:943129NEWT:1221594NEWT:990119NEWT:77216NEWT:1111708NEWT:227321NEWT:493760NEWT:281677NEWT:106590NEWT:260710NEWT:257313NEWT:3827NEWT:400772NEWT:128161NEWT:930945NEWT:1162296NEWT:106592NEWT:46704NEWT:482428NEWT:1187947NEWT:269796NEWT:10312NCBITaxon:1773NEWT:54571NEWT:9598NEWT:8030NEWT:1423772NEWT:53232NEWT:1235987NEWT:1639NEWT:188229NEWT:246437NEWT:3818NEWT:4909NEWT:759272NEWT:432359NEWT:1182263NEWT:943146NEWT:2711NEWT:537457NEWT:479249NEWT:9103NEWT:715877NEWT:862238NEWT:29159NEWT:10306NEWT:9595NEWT:8022NEWT:145943NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:29158NEWT:3633NEWT:3635NEWT:5811NEWT:3879NEWT:180923NEWT:1328881NEWT:411483NEWT:76336NEWT:3870NEWT:884019NEWT:198215NEWT:411490NEWT:200298NEWT:169963NEWT:587NEWT:588NEWT:585NEWT:343NCBITaxon:43925NEWT:225117NEWT:746128NEWT:5801NEWT:43665NEWT:212035NEWT:5802NCBITaxon:1653394NEWT:1266464NEWT:1205NEWT:214693NEWT:367830NEWT:178616NEWT:95145NEWT:375451NEWT:357NEWT:9172NEWT:2770979NEWT:2010198NEWT:1277351NEWT:1594786NEWT:457428NEWT:106178NEWT:411469NEWT:84023NEWT:1679NEWT:559292NEWT:569NEWT:411464NEWT:411460NEWT:2762NEWT:1174673NEWT:1328426NEWT:562NEWT:28035NEWT:411470NEWT:2094720NCBITaxon:2697049NCBITaxon:138NEWT:28038NEWT:1663NEWT:62141NEWT:1423NEWT:4932NEWT:3603NEWT:2756NEWT:2759NEWT:3848NEWT:3847NEWT:38293NEWT:1428NEWT:339NEWT:327159NEWT:178876NEWT:1660NEWT:2751NEWT:327160NEWT:573NEWT:571NEWT:12922NEWT:29595NEWT:1306413NEWT:241368NEWT:518766NEWT:42528NEWT:2664373NEWT:190802NEWT:9417NEWT:1216979NEWT:7237NEWT:264483NEWT:509924NEWT:28450NEWT:115104NEWT:1121114NEWT:663NEWT:8570NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:1241935NEWT:220668NEWT:1821314NEWT:1249668NEWT:426430NEWT:9407NEWT:9646NEWT:5286NEWT:7227NEWT:7469NEWT:9402NEWT:9644NEWT:415540NEWT:675060NEWT:334542NEWT:313625NEWT:27592NEWT:426428NEWT:227289NEWT:63459NEWT:1276815NEWT:40324NEWT:588858NEWT:9638NEWT:9639NCBITaxon:11071NEWT:242231NEWT:197221NEWT:7215NEWT:51453NEWT:2172103NCBITaxon:59201NEWT:7460NEWT:504457NEWT:434271NEWT:392227NEWT:747679NEWT:66712NEWT:42752NEWT:103372NEWT:746360NEWT:862751NEWT:89764NEWT:470150NEWT:9627NEWT:543734NEWT:1009714NEWT:73239NEWT:38018NEWT:226186NEWT:9870NEWT:8782NEWT:115357NEWT:435590NEWT:1902NEWT:63436NEWT:1908NEWT:1169740NEWT:13164NEWT:216129NEWT:2664179NEWT:623NEWT:7038NEWT:6192NCBITaxon:1639NCBITaxon:38727NEWT:74790NEWT:2829NEWT:366581NEWT:216599NEWT:405534NEWT:216595NEWT:40559NEWT:51208NEWT:216597NEWT:9689NEWT:8355NEWT:633NEWT:1607839NEWT:9685NEWT:7029NEWT:6182NEWT:1283300NEWT:9694NEWT:186990NEWT:632NEWT:1204551NEWT:6183NEWT:99586NEWT:630NCBITaxon:11019NEWT:334747NEWT:61235NEWT:392021NEWT:343691NEWT:1457551NEWT:15368NEWT:1491466NEWT:436486NEWT:340188NEWT:51655NEWT:170187NEWT:260707NEWT:121845NEWT:8595NEWT:7263NEWT:186763NEWT:185431NEWT:216591NEWT:29760NEWT:260704NEWT:1719NEWT:703612NEWT:260705NEWT:1202347NEWT:692672NEWT:2697049NEWT:1505907NEWT:1216981NEWT:1109443NEWT:1249885NEWT:7004NEWT:1972855NEWT:9669NCBITaxon:6073NEWT:1089448NEWT:1415167NEWT:1329349NEWT:607699NEWT:208963NEWT:208964NEWT:27337NEWT:575584NEWT:185687NEWT:3039NEWT:669202NEWT:2706014NEWT:8727NEWT:8726NEWT:10090NEWT:8729NEWT:4120NEWT:8720NEWT:5693NEWT:2186NEWT:8724NEWT:8723NEWT:283166NEWT:37334NEWT:5697NEWT:6544NCBITaxon:10357NCBITaxon:10114NCBITaxon:10359NEWT:1685931NEWT:1203258NEWT:242619NEWT:49317NEWT:557723NEWT:557722NEWT:160791NEWT:1353008NEWT:34073NEWT:696811NEWT:402042NEWT:373995NEWT:5689NEWT:8715NEWT:839NEWT:544404NEWT:92519NEWT:2173NEWT:943925NEWT:37349NEWT:4113NEWT:837NEWT:640459NEWT:36015NEWT:1232801NEWT:81866NEWT:5691NEWT:48479NEWT:61435NEWT:627025NEWT:1097677NEWT:375939NEWT:61674NEWT:2072580NEWT:669465NEWT:1117957NEWT:6526NEWT:8709NEWT:4100NEWT:1159556NEWT:5671NEWT:1076NEWT:4102NEWT:4101NEWT:6763NEWT:8700NEWT:803NEWT:29722NEWT:1692259NEWT:1486917NEWT:419947NEWT:468911NEWT:13373NEWT:817NEWT:7604NEWT:818NEWT:135588NEWT:1843183NEWT:5661NEWT:157592NEWT:51866NEWT:29719NEWT:58002NEWT:5665NEWT:6754NEWT:1179777NEWT:6997NEWT:452589NEWT:509068NEWT:5666NEWT:4577NEWT:813NEWT:5664NEWT:2157NEWT:146479NEWT:1042986NEWT:1911079NEWT:69373NEWT:1843192NEWT:206189NEWT:427577NEWT:264203NEWT:1480154NEWT:1274414NEWT:9612NEWT:38626NEWT:1286640NEWT:38865NEWT:3077NEWT:9615NEWT:10299NEWT:6100NEWT:713604NEWT:112273NEWT:1147787NEWT:1440772NEWT:1355477NEWT:8759NEWT:9605NEWT:9606NEWT:90371NEWT:641501NEWT:4153NEWT:9601NEWT:278839NEWT:35128NEWT:39966NEWT:7668NEWT:8756NEWT:915099NEWT:74940NEWT:13551NEWT:10041NEWT:9600NEWT:1450511NEWT:1143193NEWT:1122233NEWT:890382NEWT:174934NEWT:179392NEWT:2823525NEWT:115778NEWT:9838NEWT:9839NEWT:546414NEWT:227086NEWT:3055NEWT:11167NEWT:9835NEWT:7650NEWT:4146NEWT:5478NEWT:6326NEWT:7656NEWT:5476NEWT:1450520NEWT:1042311NEWT:1123352NEWT:1882945NEWT:746707NEWT:299767NEWT:235909NEWT:28835NEWT:47311NEWT:1274432NEWT:2053667NEWT:1274426NEWT:104660NEWT:9825NEWT:1274423NEWT:393765NEWT:652611NEWT:39946NEWT:9823NEWT:95602NEWT:8732NEWT:314539NEWT:39947NEWT:718274NEWT:1274420NEWT:578458NEWT:60520422739160000-0003-0066-3720