Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Fraction_Info.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91945_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91946_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91521_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91944_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91939_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91522_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91940_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91941_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91938_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91942_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91523_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91524_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91949_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91947_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91948_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Seq91943_DIA_88min_480Ex1.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/fragger_dia.paramsftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Cyto.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Mito.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Pre12K.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/Total_extract.zipftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065908/2024-07-01-decoys-reviewed-contam-UP000000589.fasprimaryOK200philippa@technion.ac.ilOded KleifeldMass SpectrometryData-independent acquisitionBottom-up proteomicsMkrn3DiaGnrh1 precursor proteinhttps://www.ebi.ac.uk/pride/archive/projects/PXD065908Cell CultureHypothalamus CellWT GT1-7 cells and those stably expressing GFP-Mkrn3 (3X 100 mm plates)were lysed and fractionated using Mitochondria separation was performed using the Mitochondria Isolation Kit for Cultured Cells (Thermo, 89874), according to the manufacturer’s instructions. After the initial 3,000g precipitation for the major “heavy” mitochondrial fraction, an additional precipitation at 12,000g was performed on the supernatant to enrich for “light” mitochondria and other organelles such as peroxisomes and lysosomes. The proteins in these two pellets, as well as in the final supernatant, were digested by trypsin and analyzed on Exploris 480 mass spectrometer (Thermo),PrideThe raw files were processed using MSFragger (https://fragpipe.nesvilab.org/docs/tutorial_fragpipe.html) with the DIA_SpecLib_Quant workflow and default parameters. Data were searched against the Mus musculus proteome (UniProt ID: UP000000589), including a decoy database to estimate the false discovery rate (FDR). Identified peptides were filtered for high confidence, top rank, and accurate mass, and only those passing a 1% FDR threshold were retained. Searches were conducted separately for each fraction or sample type.ProteomicsPhilippa MelamedThe Dr. Irving and Jeanette Benveniste Chair in Life Sciences, Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000 ISRAELPARTIALMus Musculus (mouse)okleifeld@technion.ac.ilNot availableTechnionIsraelfalseMkrn3 binds Gnrh1 mRNA to alter subcellular localization of the Gnrh1 precursor protein - DIA cell fractionsLoss-of-function mutations in MKRN3 cause central precocious puberty, and our findings show that MKRN3 regulates GnRH production by binding the Gnrh1 3′UTR and altering subcellular localization of its precursor protein through post-transcriptional mechanisms. This function likely involves interactions with RNA-binding proteins such as IGF2BP2 and may extend to other cell types and neuropeptides.2026-05-142025-07-08PXD065908NEWT:6945NEWT:3555NEWT:241368NEWT:2NEWT:157546NEWT:190802NEWT:35554NEWT:9778NEWT:150475NEWT:9417NEWT:7111NEWT:347515NEWT:1216979NEWT:307972NEWT:32046NEWT:544496NEWT:5180NEWT:256737NEWT:2042546NEWT:115104NEWT:1081927NEWT:1238993NEWT:67825NEWT:185579NEWT:43179NEWT:13076NEWT:1249668NEWT:317NEWT:55153NCBITaxon:10407NEWT:1736309NEWT:7227NEWT:7469NEWT:885318NEWT:1211601NEWT:415540NEWT:4081NEWT:876138NEWT:554NEWT:98334NEWT:426428NEWT:237561NEWT:6928NEWT:10036NEWT:7574NEWT:1351NEWT:7215NEWT:575412NEWT:29204NEWT:272563NEWT:507601NCBITaxon:79857NCBITaxon:6157NEWT:95648NEWT:3885NEWT:746360NEWT:6239NEWT:3888NEWT:1589NEWT:470150NEWT:135622NEWT:216257NEWT:6915NEWT:9986NEWT:101510NEWT:4054NEWT:3880NEWT:272559NEWT:226186NEWT:3641NEWT:383379NEWT:8782NEWT:1263854NEWT:1000589NEWT:435590NEWT:1902NEWT:85962NEWT:160488NEWT:28104NEWT:317447NEWT:7955NCBITaxon:2NEWT:985076NEWT:7959NEWT:2261NEWT:4565NEWT:1264690NEWT:515619NEWT:6192NEWT:28532NCBITaxon:38727NEWT:34305NEWT:59729NCBITaxon:183674NEWT:224308NEWT:626528NEWT:216599NEWT:139927NEWT:4558NEWT:209285NEWT:216595NEWT:243230NEWT:8355NEWT:1283NEWT:931281NEWT:4550NEWT:1000561NEWT:7029NEWT:1283300NEWT:6183NEWT:6063NEWT:334747NEWT:61235NCBITaxon:79824NEWT:4787NCBITaxon:4563NEWT:5755NEWT:3218NEWT:5759NEWT:1736231NEWT:436486NEWT:6287NEWT:2242NEWT:300641NEWT:4784NEWT:727NEWT:9796NEWT:11320NEWT:725NEWT:360106NEWT:260707NEWT:287NEWT:10117NEWT:10239NCBITaxon:6191NEWT:10116NEWT:1280NEWT:1836NEWT:1735272NEWT:83334NEWT:185431NEWT:83332NEWT:29760NEWT:260704NEWT:703612NEWT:260705NEWT:80863NEWT:44685NEWT:2697049NEWT:1148NEWT:11676NEWT:55571NEWT:100226NCBITaxon:6073NEWT:4530NEWT:4896NEWT:6279NEWT:1123869NEWT:7370NEWT:75058NEWT:83906NEWT:607699NEWT:6282NEWT:1094343NEWT:208964NEWT:1134506NEWT:575584NEWT:296543NEWT:1773NEWT:38783NEWT:8727NEWT:1895NEWT:4006NEWT:1182590NEWT:8726NEWT:6669NEWT:10090NEWT:935293NEWT:64152NEWT:749200NEWT:4120NEWT:51515NEWT:5693NEWT:8724NEWT:51511NEWT:92867NEWT:8723NEWT:990346NEWT:5334NEWT:145953NEWT:257309NEWT:100816NEWT:230741NEWT:284812NCBITaxon:10359NCBITaxon:1313NEWT:43330NEWT:242619NEWT:44544NEWT:632957NEWT:373995NEWT:5689NEWT:645463NEWT:544404NEWT:3702NEWT:129249NEWT:9925NEWT:8839NEWT:4232NEWT:990119NEWT:2758385NEWT:4113NEWT:837NEWT:11298NEWT:171101NEWT:714NEWT:421932NEWT:196627NEWT:408172NEWT:5691NEWT:408170NEWT:493760NEWT:260710NEWT:627025NEWT:400772NEWT:1097677NEWT:3708NEWT:128161NEWT:106592NEWT:536231NEWT:61674NEWT:1117957NEWT:9913NEWT:1432138NEWT:10312NEWT:1424507NEWT:4100NEWT:1076NEWT:6763NEWT:803NEWT:8030NEWT:29722NEWT:380394NEWT:1692259NEWT:1639NEWT:188229NEWT:3818NEWT:480NEWT:4909NEWT:180066NEWT:67767NEWT:46835NEWT:135588NEWT:376686NEWT:1843183NEWT:95486NEWT:58002NEWT:9103NEWT:4577NEWT:1416333NEWT:5664NEWT:2157NEWT:146479NEWT:10306NCBITaxon:2759NEWT:1911079NEWT:8022NEWT:145943NCBITaxon:4932NEWT:595536NEWT:3635NEWT:5811NEWT:235443NEWT:1480154NEWT:1274414NEWT:27606NEWT:59202NEWT:9975NEWT:3197NEWT:9615NEWT:10299NEWT:860688NEWT:411483NEWT:884019NEWT:198215NEWT:411490NEWT:169963NEWT:36329NEWT:1147787NCBITaxon:3044782NEWT:72407NEWT:476272NEWT:349741NEWT:9606NEWT:367830NEWT:157295NEWT:641501NEWT:178616NEWT:410289NEWT:373153NEWT:915099NEWT:74940NEWT:9721NEWT:1450511NEWT:360094NEWT:470NEWT:411901NEWT:1313NEWT:411469NEWT:84023NEWT:9838NEWT:105884NCBITaxon:9615NCBITaxon:5811NEWT:58334NEWT:411464NEWT:1193501NEWT:3055NEWT:6326NEWT:6689NEWT:411460NEWT:2762NEWT:5476NEWT:1174673NEWT:562NEWT:411470NEWT:33952NEWT:2094720NEWT:1274432NEWT:1274426NEWT:1423NEWT:4932NEWT:70448NEWT:9825NEWT:1274423NEWT:3603NEWT:698936NEWT:2759NEWT:3847NEWT:39946NEWT:9823NEWT:178876NEWT:9940NEWT:327160NEWT:573NEWT:521001NEWT:9031NEWT:1274420NEWT:7091NEWT:5784580000-0003-3091-7154