{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xlsx":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/P257_07_EV_ex_vivo_experimentalDesignTemplate.xlsx","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/P257_07_plasma_ex_vivo_experimentalDesignTemplate.xlsx","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/P257_09_patient_HC_BCN_BCP_experimentalDesignTemplate.xlsx","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/P257_07_BacB_ex_vivo_experimentalDesignTemplate.xlsx"],"Txt":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/checksum.txt"],"Raw":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_039_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_084_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_019.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_006.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_031.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_050.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_080.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_030.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_033.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_059.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_009.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_089.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_010.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_026.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_063.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_021.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_045_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_055.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_016_R2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_017_R2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_038_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_005.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_072.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_065.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_024.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_048.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_028_R8.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_049.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_012.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_074.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_007.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_004.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_066.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_003.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_014.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_082_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_041.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_037.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_001.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_071.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_051_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_022_R2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_021.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_018.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_001.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_008.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_016.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_047_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_057_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_011.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_078.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_018.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_061.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_035.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_012.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_062.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_081_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_025.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_032.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_005.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_075.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_011.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_008.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_076_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_007.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_014_R2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_090.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_013_R3.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_058_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_003.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_064.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_088_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_022.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_020.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_056.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_006.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_013.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_023.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_079.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_077_R3.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_029.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_036.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_020.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_040.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_067.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_054.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_004.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_086_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_015.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_002.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_015_R2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_046_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_077.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_042.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_085.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_034.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_073_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_027.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_069.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_043_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_053_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_002.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_052.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_009.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_019.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_083_x.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_068_R2.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_087.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_060.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_834_P257_07_FRH_010.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_891_P257_09_FRH_017.raw","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/BBM_829_P257_07_FRH_044.raw"],"Fasta":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/uniprot_proteome_UP000005640_homo_sapiens_download_20200717_SwissProt_20353_noDup.fasta","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/SA_all_2024_07_18.fasta"],"Other":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/P257_09_MQSearch_patient_CH_BCN_BCP.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/P257_07_MQSearch_BacBs_Ex_Vivo.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/P257_07_MQSearch_EVs_Ex_Vivo.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067303/P257_07_MQSearch_plasma_Ex_Vivo.zip"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"labhead_mail":["tina.ludwig@tum.de"],"submitter":["Christina Ludwig"],"technology_type":["Data-dependent acquisition","Mass Spectrometry","Bottom-up proteomics"],"software":[""],"submitter_keywords":[""],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD067303"],"tissue":["Blood Plasma"],"sample_protocol":["Protein preparation for proteomics EV–Miltenyi bead complexes, bMV–BacB complexes, and total plasma or patient serum proteins were lysed in 1× RIPA buffer (Abcam plc, Cambridge, United Kingdom; ab156034) supplemented with 1× ProteaseArrest™ Protease Inhibitor Cocktail (G-BIOSCIENCES, St. Louis, MO, United States), and stored at −80 °C until further use. Protein samples were then boiled at 70 °C for 10 minutes and sonicated on ice for 5 minutes. Following sonication, lysates were centrifuged at 10,000 × g at 4 °C for 30 minutes. Protein concentrations were determined using the bicinchoninic acid (BCA) assay (Thermo Fisher Scientific Inc., Waltham, United States). A minimum of 5 μg of protein from each sample was mixed with 1× Laemmli Sample Buffer (Bio-Rad Laboratories, Hercules, United States) containing 2-mercaptoethanol (Merck KGaA, Darmstadt, Germany) in a final volume of 35 μl. Prepared samples were then submitted to the proteomics core facility at the Bavarian Center for Biomolecular Mass Spectrometry for analysis.  Mass spectrometry-based proteomics In accordance with standard procedures, in-gel trypsin digestion was performed on all EV–Miltenyi bead complexes, bMV–BacB complexes, and total plasma or patient serum samples. Briefly, the samples were separated on a Nu-PAGE™ 4–12% Bis-Tris protein gel (Thermo Fisher Scientific Inc., Waltham, United States) for about 1 cm. Subsequently, the accumulated and not size-separated single protein band per sample was cut out, reduced (50 mM dithiothreitol), alkylated (55 mM chloroacetamide), and digested overnight with trypsin (Trypsin Gold, mass spectrometry grade, Promega). The dry peptide samples were resuspended in 25 μl buffer A (2% acetonitrile, 0.1% formic acid in HPLC grade water), of which 5 μl were injected per mass spectrometry (MS) measurement."],"repository":["Pride"],"quantification_method":[""],"modification":[""],"data_protocol":["LC-MS/MS data acquisition of all samples was carried out on a Dionex Ultimate 3000 RSLCnano system coupled to an Orbitrap Fusion LUMOS mass spectrometer (ThermoFisher Scientific, Bremen). Injected peptides were delivered to a trap column (ReproSil-pur C18-AQ, 5 μm, Dr. Maisch, 20 mm × 75 μm, self-packed) at a flow rate of 5 μL/min in 0.1% formic acid in HPLC grade water. After 10 minutes of loading, peptides were transferred to an analytical column (ReproSil Gold C18-AQ, 3 μm, Dr. Maisch, 450 mm × 75 μm, self-packed) for one minute, and then separated using a 50 min gradient from 4% to 32% of solvent B (0.1% FA, 5% DMSO in acetonitrile) in solvent A (0.1% FA, 5% DMSO in HPLC grade water) at 300 nL/min flow rate. The Orbitrap Fusion LUMOS mass spectrometer was operated in data-dependent acquisition (DDA) and positive ionization mode. MS1 spectra (360–1300 m/z) were recorded at a resolution of 60k using a normalized automatic gain control (AGC) target value of 100% and a maximum injection time (maxIT) of 50 msec. A cycle time of 2 sec was set. Only precursors with charge states 2 to 6 were selected, and dynamic exclusion of 30 sec was enabled. Peptide fragmentation was performed using higher energy collision-induced dissociation (HCD) and a normalized collision energy (NCE) of 30%. The precursor isolation window width was set to 1.3 m/z. MS2 Resolution was 15.000 with a normalized automatic gain control (AGC) target value of 150% and maximum injection time (maxIT) of 22 msec.  Label-Free Quantitative Proteomics and Integrated Statistical Analysis for Host–Pathogen Profiling in Sepsis Peptide identification and quantification were performed using MaxQuant software (version 1.6.3.4) with its integrated Andromeda search engine. MS/MS spectra were searched against  the Uniprot-derived protein databases from Homo sapiens (Taxon ID: 9606, UP000005640, downloaded July 2020) and Staphylococcus aureus (Taxon ID: 1280, downloaded July2024), supplemented with common contaminants using MaxQuant’s built-in contaminant database. Trypsin/P was specified as a proteolytic enzyme, and carbamidomethylated cysteine was set as a fixed modification. Oxidation of methionine and acetylation at the protein N-terminus were defined as variable modifications. Results were adjusted to a 1% false discovery rate on peptide spectrum match level and protein level employing a target-decoy approach using reversed protein sequences. LFQ intensities were further analyzed using Perseus version v2.0.10.0. We used the built-in filter functions of Perseus to filter out reverse, only identified by site and contaminant proteins and log10 transformed all protein intensities. To identify proteins reproducibly detected across sample types including EV–Miltenyi bead complexes, bMV–BacB complexes, total plasma, and patient serum—proteins were retained if they had valid values in at least 50% of biological replicates within any one group, using the \"Filter rows based on valid values\" function in Perseus. Preprocessed data were subsequently analyzed in RStudio (version: 2025.05.0+496) with R (version: 4.5.1). Differential expression analysis was conducted using two-sided t-tests for multiple group comparisons. Proteins were considered significantly regulated if they exhibited p < 0.05 and a fold change greater than 2 or less than 0.5. Volcano plots were generated using ggplot2, and significant proteins were annotated using a combined human and S. aureus protein database. Protein-level functional enrichment analysis was performed using the clusterProfiler (version: 4.14.6), ReactomePA (version: 1.50.0), and org.Hs.eg.db (version: 3.20.0) packages to identify enriched Gene Ontology (GO) terms, KEGG pathways, and Reactome pathways. Enrichment significance was evaluated using Fisher’s exact test. Results were visualized via pathway–protein heatmaps, filtered using sepsis-related biological keywords. To assess global expression trends and group separation, sparse Partial Least Squares Discriminant Analysis (sPLS-DA) was performed using the mixOmics package (version: 6.30.0). Component-based plots with group-specific clustering and confidence ellipses were generated in RStudio (version 2025.05.0+496) with R (version 4.5.1). Finally, Venn diagrams were constructed using the Venn Diagram package to visualize the overlap and uniqueness of differentially expressed proteins across clinical conditions."],"omics_type":["Proteomics"],"labhead":["Christina Ludwig"],"instrument_platform":[""],"labhead_affiliation":["Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) Technical University of Munich (TUM) Gregor-Mendel-Str. 4 85354 Freising Germany"],"submission_type":["PARTIAL"],"species":["Homo Sapiens (human)","Staphylococcus Aureus"],"publication":["Not available"],"submitter_mail":["tina.ludwig@tum.de"],"submitter_affiliation":["TU Munich"],"submitter_country":["Germany"],"additional_accession":[]},"is_claimable":false,"name":"Extracellular Vesicle Proteomics in Staphylococcus aureus-Treated Blood and Sepsis Reveals Coordinated Acute Phase, Neutrophil, and Exocytosis Responses","description":"Sepsis accounts for nearly 20% of global mortality, with antibiotic resistance worsening clinical outcomes. Rapid antibiotic administration and accurate pathogen identification remain crucial. It is well now known that extracellular vesicles (EVs) from human cells and bacterial membrane vesicles (bMVs) play a central role in the interaction between host and pathogen and represent promising biomarkers for early infections. This study investigated how antibiotic exposure alters EV responses in Staphylococcus aureus (SA) spiked blood and compared these findings with EV proteome profiles from bacteremia patients.In an in vitro model, whole blood from healthy donors was spiked with SA at a multiplicity of infection (MOI) of 0.001, treated with clinically relevant concentrations of piperacillin–tazobactam, vancomycin, or moxifloxacin, and plasma was subsequently isolated for EV analysis. EVs were isolated using the Miltenyi Pan EV Kit and analyzed by bead-based flow cytometry and high-resolution LC–MS/MS. In parallel, serum EVs from healthy controls (n = 6) and bacteremia patients (n = 12; 6 blood culture–positive and 6 culture-negative) were analyzed using the same workflow.Flow cytometry revealed increased levels of CMO⁺ CD45⁺ PanEV⁺ SA⁺ vesicles in SA-spiked samples, particularly following low-dose piperacillin–tazobactam and high-dose vancomycin treatment, despite minimal changes in vesicle size and total particle counts. Proteomic analysis of plasma EVs showed significant alterations in protein composition, including increased abundance of the SA-derived ribosomal protein rplU and host defense–associated proteins. Functional enrichment highlighted pathways related to neutrophil degranulation, vesicle-mediated transport, and antibacterial responses. In patient samples, serum EVs were enriched in acute-phase and immune-related proteins, including SERPINA1, SERPINA3, CRP, and SAA2, along with canonical EV markers such as CD81 and syntenin-1, irrespective of blood culture status.Antibiotic exposure and SA infection are associated with measurable changes in the human EV proteome, characterized by enrichment of immune and host defense–related proteins despite stable vesicle numbers. Similar EV-associated protein patterns were observed in both blood culture–positive and –negative patient samples, reflecting shared features of the systemic host response to infection and highlighting the potential of EV profiling to capture infection-associated biological signals.","dates":{"publication":"2026-05-18","submission":"2025-08-13"},"accession":"PXD067303","cross_references":{"TAXONOMY":["NEWT:330879","NEWT:377960","NEWT:1131","NEWT:2042546","NEWT:259447","NEWT:295546","NCBITaxon:2719036","NEWT:1334565","NEWT:625351","NEWT:258594","NCBITaxon:1280","NEWT:112503","NEWT:1129","NEWT:309807","NEWT:59753","NEWT:89184","NEWT:309800","NEWT:281395","NEWT:1211601","NEWT:876138","NEWT:44271","NEWT:193516","NEWT:1073882","NEWT:6819","NEWT:475174","NEWT:428386","NEWT:1117","NEWT:498257","NEWT:10036","NEWT:32264","NEWT:1590","NEWT:10034","NEWT:33114","NEWT:1596","NEWT:661410","NEWT:638632","NEWT:224326","NEWT:376619","NCBITaxon:79857","NEWT:1096976","NEWT:3765","NEWT:3767","NEWT:1589","NEWT:135622","NEWT:672127","NEWT:35786","NEWT:1580","NEWT:399784","NEWT:96731","NEWT:656064","NEWT:1582","NEWT:1584","NEWT:36630","NEWT:1585","NEWT:383379","NEWT:418106","NCBITaxon:12721","NEWT:10029","NEWT:913645","NEWT:641809","NEWT:1392488","NEWT:38815","NEWT:317447","NEWT:4688","NEWT:7719","NEWT:5530","NEWT:868565","NEWT:135674","NEWT:79329","NEWT:30069","NEWT:12637","NEWT:59729","NEWT:2164133","NEWT:295105","NEWT:108061","NEWT:60711","NEWT:224308","NEWT:3347","NEWT:49928","NEWT:160621","NEWT:212790","NEWT:110368","NEWT:1310161","NEWT:77133","NEWT:145481","NEWT:1310165","NEWT:29058","NCBITaxon:79824","NEWT:410658","NEWT:5516","NEWT:44688","NEWT:44689","NEWT:498211","NEWT:347256","NEWT:5518","NEWT:398007","NEWT:1527468","NEWT:498217","NEWT:498216","NEWT:11320","NEWT:246196","NEWT:246197","NEWT:145458","NEWT:1310173","NEWT:44685","NEWT:161934","NEWT:1148","NEWT:5508","NEWT:3329","NEWT:419481","NEWT:5507","NEWT:410661","NEWT:1140","NCBITaxon:2157","NEWT:2066563","NEWT:1143","NEWT:1287689","NEWT:1094343","NEWT:1462472","NEWT:1336795","NEWT:644042","NEWT:150808","NEWT:1182590","NEWT:3712","NEWT:3711","NEWT:3714","NCBITaxon:37162","NEWT:270643","NEWT:2065263","NEWT:177437","NEWT:10891","NEWT:10418","NEWT:118698","NEWT:1616117","NEWT:118696","NEWT:34865","NEWT:52283","NEWT:28174","NEWT:284812","NEWT:8175","NEWT:43330","NEWT:1603293","NEWT:44664","NEWT:43346","NEWT:3702","NEWT:1245466","NEWT:3704","NEWT:244366","NEWT:1246791","NEWT:176597","NEWT:118694","NEWT:2850","NEWT:118691","NEWT:34871","NEWT:33548","NEWT:52299","NEWT:8165","NEWT:96794","NEWT:3708","NEWT:332648","NEWT:44670","NEWT:926571","NEWT:536231","NEWT:376219","NEWT:219813","NEWT:1510","NEWT:460519","NEWT:1515","NEWT:572307","NEWT:1432138","NEWT:1424507","NEWT:1194599","NEWT:272844","NEWT:1348799","NEWT:33995","NEWT:55784","NEWT:1303443","NEWT:9483","NEWT:485","NEWT:56636","NEWT:2499525","NEWT:2853422","NEWT:487","NEWT:1538553","NEWT:1679718","NEWT:480","NEWT:67767","NEWT:46835","NEWT:109757","NEWT:582580","NEWT:1185650","NEWT:216778","NEWT:1502","NEWT:128017","NEWT:376686","NEWT:237610","NEWT:95486","EFO:0001352","NEWT:119562","NEWT:1883446","NEWT:1233435","NEWT:118223","NEWT:11757","NEWT:987059","NEWT:109760","NEWT:29031","NEWT:317047","NEWT:1509","NEWT:491","NEWT:460523","NEWT:45954","NEWT:235443","NEWT:1579","NEWT:1757312","NEWT:85694","NEWT:109786","NEWT:108458","NEWT:5936","NEWT:320637","NEWT:1078283","NEWT:3750","NEWT:983964","NEWT:11706","NEWT:32644","NEWT:527796","NEWT:499175","NEWT:109779","NEWT:3745","NEWT:1715989","NCBITaxon:4751","NEWT:3747","NEWT:1116234","NEWT:1255228","NEWT:410289","NEWT:191701","NEWT:373153","NEWT:1640278","NEWT:472","NEWT:940825","NEWT:29459","NEWT:529507","NEWT:1071661","NEWT:28128","NEWT:470","NEWT:5911","NEWT:748693","NCBITaxon:50557","NEWT:39251","NEWT:29491","NEWT:101841","NEWT:446","NEWT:153481","NEWT:2014887","NEWT:33952","NEWT:11723","NEWT:445","NEWT:153009","NEWT:261756","NEWT:166313","NEWT:63366","NEWT:63367","NEWT:215402","NEWT:1547","NEWT:1290454","NEWT:906914","NEWT:28141","NEWT:9031","NEWT:27292","NEWT:1050722","NEWT:3728","NEWT:108931","NEWT:1293497","NEWT:1055524","NEWT:548","NEWT:150475","NEWT:267872","NEWT:381666","NEWT:172269","NEWT:9534","NEWT:2126348","NEWT:5180","NEWT:256737","NEWT:9541","NEWT:8694","NEWT:33936","NEWT:8692","NEWT:2903","NEWT:185579","NEWT:13076","NEWT:207340","NEWT:1006581","NEWT:8673","NEWT:33940","NEWT:550","NEWT:33941","NEWT:554","NEWT:451516","NEWT:552","NEWT:1325291","NEWT:42410","NEWT:36185","NEWT:1054211","NEWT:1225786","NEWT:771870","NEWT:51338","NEWT:9995","NEWT:575412","NEWT:28112","NEWT:49012","NEWT:6493","NEWT:10832","NEWT:6494","NEWT:6491","NEWT:507601","NEWT:520","NEWT:186441","NEWT:643680","NEWT:214092","NCBITaxon:6157","NEWT:13095","NEWT:162425","NEWT:104105","NEWT:216257","NEWT:9986","NEWT:8654","NEWT:8658","NEWT:1268063","NEWT:8657","NEWT:9983","NEWT:8655","NEWT:5147","NEWT:28108","NEWT:28104","NEWT:407821","NEWT:568703","NCBITaxon:2","NEWT:568708","NEWT:1613","NEWT:986","NEWT:52641","NEWT:28532","NEWT:353152","NEWT:298176","NEWT:353153","NEWT:227377","NEWT:1774284","NEWT:40674","NEWT:1194669","NEWT:74426","NEWT:443906","NEWT:13037","NEWT:519","NEWT:2510939","NEWT:7394","NEWT:6063","NEWT:1328388","NEWT:105487","NEWT:1548728","NEWT:667127","NEWT:9557","NEWT:377586","NEWT:300641","NEWT:39655","NEWT:9554","NEWT:38323","NEWT:475932","NEWT:256318","NEWT:206411","NCBITaxon:6191","NEWT:229533","NEWT:214053","NEWT:28550","NEWT:80863","NEWT:90675","NEWT:52639","NEWT:52638","NEWT:57075","NEWT:1666905","NEWT:8697","NEWT:861109","NEWT:8695","NEWT:884204","NEWT:1045775","NEWT:1123869","NEWT:9544","NEWT:9545","NEWT:979","NEWT:7370","NEWT:83906","NEWT:99488","NEWT:1134506","NEWT:255470","NEWT:38783","NEWT:6426","NEWT:33090","NEWT:9935","NEWT:287889","NEWT:305959","NEWT:1028307","NEWT:13489","NEWT:29829","NEWT:92867","NEWT:92866","NCBITaxon:3055","NEWT:51750","NEWT:202950","NEWT:441772","NEWT:1293091","NEWT:48106","NEWT:202956","NEWT:295027","NCBITaxon:11320","NEWT:632957","NEWT:9925","NCBITaxon:9606","NEWT:90690","NEWT:1436183","NEWT:300273","NEWT:4232","NEWT:416348","NEWT:11298","NEWT:4236","NEWT:7742","NEWT:196627","NEWT:1076878","NEWT:200308","NEWT:200302","NEWT:870435","NEWT:58370","NEWT:9913","NEWT:9915","NEWT:105841","NEWT:2666255","NEWT:999810","NCBITaxon:5693","NEWT:28995","NEWT:2144189","NEWT:1392998","NEWT:380394","NEWT:1150621","NEWT:226900","NEWT:1266738","NEWT:231490","NEWT:244704","NEWT:7725","NEWT:9909","NEWT:9902","NEWT:430615","NEWT:72664","NEWT:563041","NEWT:36599","NEWT:41949","NEWT:249161","NEWT:326423","NEWT:452467","NEWT:198822","NEWT:36111","NEWT:326424","NEWT:1678078","NEWT:749906","NEWT:418985","NEWT:749907","NEWT:150847","NEWT:2023717","NEWT:29835","NEWT:431947","NEWT:69014","NEWT:142809","NEWT:1095183","NEWT:302409","NEWT:130821","NEWT:27606","NEWT:1519788","NEWT:59202","NEWT:9975","NEWT:8643","NEWT:1801631","NEWT:1159899","NEWT:502780","NEWT:860688","NEWT:13443","NEWT:35231","NEWT:1774208","NEWT:8644","NEWT:1125630","NEWT:5141","NEWT:46360","NCBITaxon:3044782","NEWT:202914","NEWT:160235","NEWT:502779","NEWT:297246","NEWT:103690","NEWT:8639","NEWT:1928434","NEWT:8637","NEWT:1114970","NEWT:2096","NEWT:285272","NEWT:192222","NEWT:9963","NEWT:1617910","NEWT:197911","NEWT:197912","NEWT:637379","NEWT:12107","NEWT:2785785","NEWT:411901","NEWT:1298722","NEWT:8626","NEWT:186801","NEWT:105884","NCBITaxon:9615","NEWT:8621","NEWT:58334","NEWT:58331","NEWT:6446","NEWT:33038","NEWT:8624","NEWT:5116","NEWT:6447","NEWT:8623","NEWT:5597","NCBITaxon:3077","NEWT:194439","NCBITaxon:12637","NEWT:38727","NEWT:218496","NEWT:8618","NEWT:70448","NEWT:300251","NEWT:9940","NEWT:13451","NEWT:1247190","NEWT:118702","NEWT:118700","NEWT:2126719","NEWT:118705","NEWT:6945","NEWT:2104","NEWT:2627626","NEWT:6943","NEWT:157546","NEWT:870726","NEWT:91374","NEWT:91373","NEWT:1414448","NEWT:474186","NEWT:54195","NEWT:10136","NEWT:38942","NEWT:4762","NEWT:6941","NEWT:307972","NEWT:2102","NEWT:34","NEWT:38949","NEWT:1299114","NEWT:1590841","NEWT:60876","NEWT:1276473","NEWT:2282334","NEWT:1247","NEWT:70667","NEWT:869728","NEWT:6938","NCBITaxon:39054","NEWT:1736309","NEWT:4751","NEWT:36774","NEWT:194963","NEWT:860918","NEWT:77298","NEWT:15819","NEWT:109461","NEWT:1085956","NEWT:6928","NEWT:1239","NEWT:36745","NEWT:400727","NEWT:272560","NEWT:272563","NEWT:11009","NEWT:913998","NEWT:68335","NEWT:3885","NEWT:3888","NEWT:649408","NEWT:6915","NEWT:101510","NEWT:3880","NEWT:272559","NEWT:216816","NEWT:153151","NEWT:2681598","NEWT:1220","NEWT:515849","NEWT:10141","NEWT:95767","NEWT:466585","NEWT:1000589","NEWT:85963","NEWT:29","NEWT:85962","NEWT:42197","NEWT:6918","NEWT:681196","NEWT:143361","NEWT:1223501","NEWT:5656","NEWT:1292","NEWT:1295","NEWT:1299","NEWT:424718","NEWT:316407","NEWT:1794699","NCBITaxon:183674","NEWT:167387","NEWT:84645","NEWT:1123294","NEWT:6978","NEWT:209285","NEWT:211586","NEWT:747078","NEWT:1282","NEWT:1283","NEWT:1000561","NEWT:253836","NEWT:2133","NEWT:6973","NEWT:176279","NEWT:294381","NEWT:197","NEWT:1390363","NEWT:35889","NEWT:406072","NEWT:351660","NEWT:29176","NEWT:201319","NEWT:1073569","NEWT:4787","NEWT:751585","NEWT:113549","NEWT:46503","NEWT:180788","NEWT:159749","NEWT:1270","NEWT:388919","NEWT:130081","NEWT:4784","NEWT:360106","NEWT:4785","NEWT:360104","NEWT:10117","NEWT:401614","NEWT:10116","NEWT:1280","NEWT:83333","NEWT:83334","NEWT:242718","NEWT:83332","NEWT:161","NEWT:242715","NEWT:2951803","NEWT:100226","NEWT:1263","NEWT:176299","NEWT:1654360","NEWT:54126","NEWT:75058","NEWT:13616","NEWT:13613","NEWT:296543","NEWT:173","NEWT:316435","NEWT:42157","NEWT:165179","NEWT:1034304","NEWT:105023","NEWT:866628","NEWT:535026","NEWT:749200","NEWT:1036967","NEWT:266264","NEWT:272942","NEWT:272943","NEWT:1882682","NEWT:145953","NEWT:257309","NEWT:33657","NEWT:203124","NEWT:230741","NEWT:8296","NEWT:117008","NEWT:167748","NEWT:1163394","NEWT:645463","NEWT:749696","NEWT:320549","NEWT:990119","NEWT:77216","NEWT:1111708","NEWT:11855","NEWT:998086","NEWT:228410","NEWT:493760","NEWT:93163","NEWT:257313","NEWT:3827","NEWT:400772","NEWT:257310","NEWT:128161","NEWT:930945","NEWT:332521","NEWT:106350","NEWT:29170","NEWT:269796","NEWT:54571","NEWT:412754","NEWT:9598","NEWT:52389","NEWT:2665964","NEWT:1423772","NEWT:51056","NEWT:35815","NEWT:53232","NEWT:1235987","NEWT:632138","NCBITaxon:2509511","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:432359","NEWT:9103","NEWT:715877","NEWT:862238","NEWT:29159","NEWT:1155748","NEWT:9593","NEWT:8260","NEWT:9595","NEWT:2665958","NEWT:246200","NEWT:6087","NEWT:145943","NEWT:595536","NEWT:1134822","NEWT:240906","NEWT:89920","NEWT:3809","NEWT:29158","NEWT:44744","NEWT:3879","NEWT:1328881","NEWT:411483","NEWT:76336","NEWT:3870","NEWT:884019","NEWT:411490","NEWT:169963","NEWT:587","NEWT:588","NEWT:585","NCBITaxon:43925","NEWT:746128","NEWT:212035","NCBITaxon:1653394","NEWT:1205","NEWT:214693","NEWT:1207","NEWT:45609","NEWT:239935","NEWT:1680","NEWT:280148","NEWT:9172","NEWT:2770979","NEWT:112","NEWT:423477","NEWT:1277351","NEWT:1594786","NEWT:40366","NEWT:3854","NEWT:214687","NEWT:411469","NEWT:1679","NEWT:333849","NEWT:214684","NEWT:1758543","NEWT:569","NEWT:411464","NEWT:411460","NEWT:1328426","NEWT:562","NEWT:411470","NEWT:10515","NEWT:2094720","NCBITaxon:2697049","NEWT:294750","NEWT:1663","NEWT:214675","NEWT:62141","NEWT:65651","NEWT:3848","NEWT:3847","NEWT:198466","NEWT:327159","NEWT:1045010","NEWT:178876","NEWT:1660","NEWT:327160","NEWT:573","NEWT:1131757","NEWT:571","NEWT:142479","NEWT:29595","NEWT:241368","NEWT:42528","NEWT:1969471","NEWT:9657","NEWT:150596","NEWT:30540","NEWT:264483","NEWT:439733","NEWT:76777","NEWT:30545","NEWT:76773","NEWT:1121114","NEWT:663","NEWT:1081927","NEWT:1238993","NEWT:67825","NEWT:187878","NEWT:1504802","NEWT:1821314","NEWT:103351","NEWT:40354","NEWT:1249668","NEWT:426430","NEWT:37181","NEWT:929439","NEWT:9646","NEWT:5286","NEWT:7469","NEWT:9643","NEWT:9644","NEWT:426428","NEWT:63459","NEWT:2652157","NEWT:2652158","NEWT:40353","NEWT:40324","NEWT:588858","NEWT:9638","NEWT:9639","NCBITaxon:11071","NEWT:693977","NEWT:242231","NEWT:197221","NEWT:528354","NEWT:51453","NEWT:1279085","NEWT:28231","NEWT:7460","NEWT:504457","NEWT:392227","NEWT:1920175","NEWT:1004024","NEWT:103372","NEWT:862751","NEWT:470150","NEWT:9627","NEWT:1009714","NEWT:73239","NEWT:38018","NEWT:226186","NEWT:381518","NEWT:659","NEWT:30532","NEWT:8782","NEWT:30538","NEWT:115357","NEWT:5270","NEWT:63436","NEWT:63433","NEWT:13164","NEWT:1601067","NEWT:129554","NEWT:40302","NEWT:1249615","NEWT:343232","NEWT:343237","NEWT:2664179","NEWT:623","NEWT:217219","NEWT:7038","NEWT:6192","NCBITaxon:1639","NEWT:3915","NEWT:1581419","NEWT:405534","NEWT:9689","NEWT:8355","NEWT:633","NEWT:9685","NEWT:1218948","NEWT:7029","NEWT:9688","NEWT:6182","NEWT:9694","NEWT:604162","NEWT:1046723","NEWT:186990","NEWT:8364","NEWT:6185","NEWT:632","NEWT:6183","NEWT:1423800","NEWT:630","NCBITaxon:11019","NEWT:334747","NEWT:3907","NEWT:3906","NEWT:27315","NEWT:61235","NEWT:343691","NEWT:1457551","NEWT:15368","NEWT:76717","NEWT:13180","NEWT:1491466","NEWT:340188","NEWT:170187","NEWT:1755437","NEWT:1042399","NEWT:9681","NEWT:1718","NEWT:1719","NEWT:692672","NEWT:2697049","NEWT:568813","NEWT:1109443","NEWT:267566","NEWT:58042","NEWT:7004","NEWT:9669","NCBITaxon:6073","NEWT:1089448","NEWT:1329349","NEWT:1089444","NEWT:607699","NEWT:6161","NEWT:206542","NEWT:27337","NEWT:575584","NEWT:186536","NEWT:257771","NEWT:185687","NEWT:3039","NEWT:2706014","NEWT:8727","NEWT:8726","NEWT:8725","NEWT:10090","NEWT:8729","NEWT:8720","NEWT:5693","NEWT:2186","NEWT:8724","NEWT:8723","NEWT:283166","NEWT:439334","NEWT:8722","NEWT:5697","NEWT:6544","NEWT:218192","NCBITaxon:10114","NEWT:1685931","NEWT:242619","NEWT:557722","NEWT:160791","NEWT:1353008","NEWT:34073","NEWT:402042","NEWT:373995","NEWT:5689","NEWT:8715","NEWT:544404","NEWT:287535","NEWT:2173","NEWT:2175","NEWT:1232801","NEWT:5691","NEWT:61674","NEWT:2072580","NEWT:669465","NEWT:1117957","NEWT:6526","NEWT:8709","NEWT:1064539","NEWT:8707","NEWT:1306274","NEWT:5671","NEWT:13146","NEWT:6523","NEWT:8700","NEWT:1692259","NEWT:1486917","NEWT:1843183","NEWT:5661","NEWT:157592","NEWT:3482","NEWT:52714","NEWT:51866","NEWT:58002","NEWT:13136","NEWT:5660","NEWT:5665","NEWT:1179777","NEWT:6997","NEWT:5666","NEWT:1746091","NEWT:5664","NEWT:2157","NEWT:70125","NEWT:146479","NEWT:905079","NEWT:1843192","NEWT:206189","NEWT:2160","NEWT:295139","NEWT:27779","NEWT:181214","NEWT:6108","NEWT:1720309","NEWT:9612","NEWT:1286640","NEWT:38865","NEWT:3077","NEWT:9615","NEWT:6100","NEWT:713604","NEWT:3076","NEWT:112273","NEWT:13563","NEWT:33178","NEWT:1147787","NEWT:39717","NEWT:62977","NEWT:2562269","NEWT:1440772","NEWT:8759","NEWT:9605","NEWT:9606","NEWT:27717","NEWT:641501","NEWT:9601","NEWT:8752","NEWT:34038","NEWT:9604","NEWT:8756","NEWT:74940","NEWT:13551","NEWT:10041","NEWT:9600","NEWT:1450511","NEWT:439375","NEWT:1857323","NEWT:890382","NEWT:174934","NEWT:179392","NEWT:2823525","NEWT:115778","NEWT:1538186","NEWT:82996","NEWT:227086","NEWT:3055","NEWT:195883","NEWT:1450520","NEWT:1042311","NEWT:1123352","NEWT:741258","NEWT:82525","NEWT:29920","NEWT:235909","NEWT:47311","NCBITaxon:813","NEWT:127785","NEWT:708248","NEWT:47314","NEWT:104660","NEWT:652611","NEWT:94993","NEWT:8732","NEWT:763037","NEWT:1250232","NEWT:1052588","NEWT:1377","NEWT:3555","NEWT:71647","NEWT:260201","NEWT:35554","NCBITaxon:12939","NEWT:935692","NEWT:1322343","NEWT:32046","NEWT:2222","NEWT:59523","NEWT:544496","NEWT:59529","NEWT:32049","NEWT:1392696","NEWT:762376","NEWT:45351","NEWT:445974","NEWT:43179","NEWT:180454","NEWT:3544","NEWT:5722","NEWT:7901","NEWT:32051","NEWT:10245","NEWT:55153","NEWT:10243","NEWT:351581","NEWT:43186","NEWT:70779","NEWT:428146","NEWT:498019","NEWT:1351","NEWT:1438992","NEWT:1352","NEWT:32025","NEWT:2649997","NEWT:1147161","NEWT:43150","NEWT:69781","NEWT:85831","NEWT:263737","NEWT:1333499","NEWT:5702","NEWT:95648","NEWT:1349","NEWT:100787","NEWT:1401257","NEWT:44491","NEWT:27933","NEWT:556484","NEWT:94328","NEWT:7955","NEWT:7959","NEWT:645159","NEWT:2261","NEWT:94323","NEWT:31156","NEWT:3112","NEWT:8801","NEWT:4442","NEWT:192875","NEWT:1214916","NEWT:284218","NEWT:1316931","NEWT:45796","NEWT:511145","NEWT:213401","NEWT:931281","NEWT:4432","NEWT:5762","NEWT:5763","NEWT:118099","NEWT:32019","NEWT:658457","NEWT:420247","NEWT:35525","NEWT:295358","NEWT:2246","NEWT:2652724","NEWT:5757","NEWT:68415","NCBITaxon:4563","NEWT:5755","NEWT:44447","NEWT:752555","NEWT:39097","NEWT:347495","NEWT:5759","NEWT:1736231","NEWT:1392","NEWT:35974","NEWT:2242","NEWT:1396","NEWT:286","NEWT:391619","NEWT:470921","NEWT:287","NEWT:1367269","NEWT:633149","NEWT:1149786","NEWT:10239","NEWT:68895","NEWT:3567","NEWT:68888","NEWT:4897","NEWT:5744","NEWT:72901","NEWT:213863","NEWT:55571","NEWT:35500","NEWT:1235816","NEWT:2041044","NEWT:3562","NEWT:4896","NEWT:5740","NEWT:1262161","NEWT:343904","NEWT:1314781","NEWT:1390","NEWT:11557","NEWT:294","NEWT:1773","NEWT:82380","NEWT:935293","NEWT:375146","NEWT:471876","NEWT:1772","NEWT:263","NEWT:11988","NEWT:400667","NEWT:408169","NEWT:36809","NEWT:47946","NEWT:105135","NEWT:1764","NEWT:39087","NEWT:109974","NEWT:413996","NEWT:243277","NEWT:7067","NEWT:586722","NEWT:243273","NEWT:1986198","NEWT:274","NEWT:7070","NCBITaxon:287","NEWT:408172","NEWT:408170","NEWT:283734","NEWT:1436733","NEWT:42229","NEWT:1247411","NEWT:9238","NEWT:13708","NEWT:333760","NEWT:34607","NEWT:1513458","NEWT:1233681","NEWT:2212966","NCBITaxon:40559","NEWT:506599","NEWT:81077","NEWT:84588","NEWT:1279007","NEWT:85433","NEWT:65349","NEWT:300852","NEWT:564933","NEWT:508771","NEWT:1121405","NEWT:253","NCBITaxon:2759","NEWT:93061","NEWT:93062","NEWT:1284813","NEWT:34613","NEWT:7054","NCBITaxon:4932","NEWT:1748","NEWT:108673","NEWT:2764168","NEWT:943274","NEWT:1488406","NEWT:644223","NEWT:1263082","NEWT:272623","NEWT:1288389","NEWT:272624","NEWT:886559","NEWT:55521","NEWT:118499","NEWT:1130798","NEWT:55529","NEWT:256136","NEWT:476272","NEWT:195051","NEWT:3988","NEWT:561007","NEWT:649908","NEWT:1001135","NEWT:147828","NEWT:3981","NEWT:3983","NEWT:203696","NEWT:223283","NEWT:352472","NEWT:235","NEWT:505235","NEWT:55518","NEWT:97441","NEWT:1154756","NEWT:1202531","NEWT:1202532","NEWT:360094","NEWT:1408223","NEWT:1408224","NEWT:41364","NEWT:1311","NEWT:1313","NEWT:1218097","NEWT:1080348","NEWT:1314","NEWT:39491","NEWT:212142","NCBITaxon:5811","NEWT:3972","NEWT:1499105","NEWT:686","NEWT:192812","NEWT:408188","NEWT:571256","NEWT:40483","NEWT:688333","NEWT:1119529","NEWT:1303","NCBITaxon:548681","NEWT:318586","NCBITaxon:5820","NEWT:272634","NEWT:198107","NEWT:1979401","NEWT:9267","NEWT:155892","NEWT:691","NEWT:28384","NEWT:167879","NEWT:1872122","NEWT:1308","NEWT:7091","NEWT:1034015","NEWT:760568","NEWT:141262","NEWT:1737068","NEWT:1813","NEWT:4087","NEWT:9778","NEWT:306","NEWT:505693","NEWT:303","NEWT:97477","NEWT:7111","NEWT:113287","NEWT:347515","NEWT:435258","NEWT:6269","NEWT:4090","NEWT:4092","NEWT:4093","NEWT:786","NEWT:4096","NEWT:208895","NEWT:941442","NEWT:536088","NEWT:311553","NEWT:1226408","NEWT:43984","NEWT:7108","NEWT:40479","NEWT:43989","NEWT:4076","NEWT:142615","NEWT:317","NEWT:885319","NEWT:293939","NEWT:497515","NEWT:885318","NEWT:39488","NEWT:4081","NEWT:273068","NEWT:1316582","NEWT:227166","NEWT:98334","NEWT:231663","NEWT:4084","NEWT:63577","NEWT:7574","NEWT:1715256","NEWT:30640","NEWT:348824","NEWT:929793","NEWT:1507179","NEWT:29204","NEWT:114155","NEWT:4072","NEWT:1337056","NEWT:4073","NEWT:634955","NEWT:6239","NEWT:102169","NEWT:208893","NEWT:4054","NEWT:301301","NEWT:98351","NEWT:1263854","NEWT:118503","NEWT:2059687","NEWT:160488","NEWT:63558","NEWT:61376","NEWT:207559","NEWT:107401","NEWT:85057","NEWT:985076","NEWT:1215323","NCBITaxon:565995","NEWT:7159","NEWT:267671","NEWT:72491","NEWT:7160","NEWT:8496","NEWT:7165","NEWT:1257118","NEWT:1387333","NEWT:8010","NEWT:1315282","NEWT:7162","NEWT:1859","NEWT:27448","NEWT:2708","NEWT:38111","NEWT:235612","NEWT:243232","NEWT:243230","NEWT:936053","NEWT:1080772","NEWT:8479","NEWT:8485","NEWT:119856","NEWT:72000","NEWT:119857","NEWT:49240","NEWT:161398","NEWT:2893330","NCBITaxon:3707","NEWT:391295","NEWT:1170828","NEWT:104395","NEWT:93934","NEWT:9315","NEWT:6289","NEWT:38568","NEWT:6287","NEWT:38569","NEWT:727","NEWT:9796","NEWT:118562","NEWT:725","NEWT:7137","NEWT:469008","NEWT:9321","NEWT:596153","NEWT:1836","NCBITaxon:10298","NEWT:1652998","NEWT:105231","NEWT:104385","NEWT:74649","NEWT:1037924","NEWT:206672","NEWT:9788","NEWT:4097","NEWT:9305","NEWT:436113","NEWT:71139","NEWT:9785","NEWT:6279","NEWT:1195774","NEWT:9793","NCBITaxon:780","NCBITaxon:1502","NEWT:6282","NEWT:27457","NEWT:4006","NEWT:1872635","NCBITaxon:5476","NEWT:113208","NEWT:6669","NEWT:1215360","NEWT:89453","NEWT:1114301","NEWT:51515","NEWT:13249","NEWT:588596","NEWT:51511","NEWT:8845","NEWT:7998","NEWT:419610","NEWT:5334","NEWT:382352","NEWT:413071","NCBITaxon:10239","NEWT:332097","NCBITaxon:714","NEWT:1076620","NEWT:11051","NEWT:89462","NEWT:452680","NEWT:8839","NEWT:4472","NEWT:2758385","NEWT:171101","NEWT:714","NEWT:5322","NEWT:568996","NEWT:421932","NEWT:312017","NEWT:576131","NEWT:553470","NCBITaxon:10243","NEWT:284593","NEWT:100392","NEWT:655819","NEWT:5315","NEWT:11082","NEWT:265872","NEWT:59463","NEWT:337330","NEWT:2285","NEWT:2287","NEWT:6645","NEWT:251229","NEWT:981087","NEWT:180066","NEWT:46147","NCBITaxon:6312","NEWT:11072","NEWT:174633","NEWT:52830","NEWT:1416333","NEWT:35024","NEWT:135461","NEWT:7962","NEWT:99287","NEWT:1817808","NEWT:145262","NEWT:145263","NEWT:296587","NEWT:694569","NEWT:9739","NEWT:111069","NEWT:1379791","NEWT:51953","NEWT:57267","NEWT:3197","NEWT:13686","NEWT:2750454","NEWT:264561","NEWT:1402135","NEWT:36329","NEWT:62615","NEWT:1555636","NEWT:1333550","NEWT:646099","NEWT:72407","NEWT:352914","NEWT:349741","NEWT:409859","NEWT:157295","NEWT:1263871","NEWT:509169","NEWT:6210","NEWT:9721","NEWT:137221","NEWT:565050","NEWT:409860","NEWT:92683","NEWT:1298968","NEWT:5353","NEWT:1034809","NEWT:452659","NEWT:1193501","NEWT:51930","NEWT:1193502","NEWT:6689","NEWT:194675","NEWT:298315","NEWT:392547","NEWT:46105","NEWT:104782","NEWT:10181","NEWT:9704","NEWT:1049056","NEWT:698936","NEWT:13216","NEWT:1009874","NEWT:4498","NEWT:8855","NEWT:11034","NEWT:3168","NEWT:10185","NEWT:78168","NEWT:521001","NEWT:192011","NEWT:340912","NEWT:1813887","NEWT:224911","NEWT:1090946","NCBITaxon:11552","NEWT:267608","NEWT:334249","NEWT:184922","NEWT:6703","NEWT:2","NEWT:35670","NEWT:511693","NEWT:5855","NEWT:4528","NEWT:35677","NEWT:136841","NEWT:1493","NEWT:10376","NEWT:1496","NEWT:3674","NEWT:5851","NEWT:660469","NEWT:935568","NEWT:1365186","NEWT:1813019","NEWT:45474","NEWT:48500","NEWT:4513","NEWT:5843","NEWT:110205","NEWT:1117645","NEWT:310915","NCBITaxon:10407","NEWT:1000364","NEWT:10360","NEWT:1403536","NEWT:1427524","NEWT:47664","NEWT:260799","NEWT:317548","NEWT:106198","NEWT:2323","NEWT:3654","NEWT:237561","NEWT:5833","NEWT:70415","NEWT:3659","NEWT:59620","NEWT:499229","NEWT:31298","NEWT:1204162","NEWT:1532734","NEWT:82688","NEWT:5823","NEWT:5821","NEWT:44139","NCBITaxon:4895","NEWT:570417","NCBITaxon:4896","NEWT:675512","NEWT:315393","NEWT:3649","NEWT:28903","NEWT:910263","NEWT:28909","NEWT:3641","NEWT:79698","NCBITaxon:5796","NEWT:12993","NEWT:398580","NEWT:4565","NEWT:1264690","NEWT:515619","NEWT:170721","NEWT:34305","NEWT:77020","NEWT:1908323","NEWT:83558","NEWT:44586","NEWT:626528","NEWT:139927","NEWT:4558","NEWT:5888","NEWT:431241","NEWT:4550","NEWT:4555","NEWT:443226","NEWT:144036","NEWT:144030","NEWT:288705","NEWT:392815","NEWT:3217","NEWT:4547","NEWT:3218","NEWT:556182","NEWT:374990","NEWT:36941","NEWT:1432059","NEWT:154293","NEWT:156471","NEWT:3694","NEWT:156476","NEWT:4543","NEWT:210007","NEWT:1857017","NEWT:10359","NEWT:1358430","NEWT:10358","NEWT:1381693","NEWT:1735272","NEWT:109298","NEWT:317513","NEWT:707678","NEWT:580240","NEWT:294128","NEWT:11676","NEWT:1917265","NEWT:55210","NEWT:718308","NEWT:4530","NEWT:5861","NEWT:44577","NEWT:1895","NEWT:2558360","NEWT:64152","NEWT:4924","NEWT:2748","NEWT:8049","NEWT:33892","NEWT:99931","NEWT:9378","NEWT:990346","NEWT:100816","NEWT:192952","NEWT:1640346","NCBITaxon:1313","NEWT:29385","NEWT:44544","NEWT:529818","NEWT:400540","NEWT:382","NEWT:4911","NEWT:152480","NEWT:129249","NEWT:1888","NEWT:1077286","NEWT:943129","NEWT:131567","NEWT:1221594","NEWT:10760","NEWT:1524260","NEWT:227321","NEWT:192944","NEWT:492670","NEWT:281677","NEWT:106590","NEWT:260710","NEWT:4918","NEWT:1162296","NEWT:106592","NEWT:596645","NEWT:46704","NEWT:42345","NEWT:482428","NEWT:1187947","NEWT:4903","NEWT:10312","NCBITaxon:1773","NEWT:323848","NEWT:190304","NEWT:1432499","NEWT:8030","NEWT:246432","NEWT:246437","NEWT:36909","NEWT:4909","NEWT:1279365","NEWT:759272","NEWT:1182263","NEWT:943146","NEWT:646532","NEWT:2711","NEWT:537457","NEWT:479249","NEWT:435623","NEWT:33889","NEWT:10306","NEWT:12967","NEWT:8022","NEWT:42353","NEWT:593117","NEWT:3633","NEWT:319761","NEWT:3635","NEWT:5811","NEWT:86662","NEWT:180923","NEWT:2050920","NEWT:1451","NEWT:198215","NEWT:200298","NEWT:12916","NEWT:1400870","NEWT:343","NEWT:84005","NEWT:225117","NEWT:34708","NEWT:8090","NEWT:537012","NEWT:5801","NEWT:43665","NEWT:5802","NEWT:4952","NEWT:1266464","NEWT:367830","NEWT:1093790","NEWT:178616","NEWT:95145","NEWT:358","NEWT:375451","NEWT:54788","NEWT:357","NEWT:74381","NEWT:2010198","NCBITaxon:186538","NEWT:457428","NEWT:106178","NEWT:316275","NEWT:84023","NEWT:589904","NEWT:559292","NEWT:316273","NEWT:2762","NEWT:1174673","NEWT:28035","NEWT:1313166","NEWT:690307","NCBITaxon:138","NEWT:28038","NEWT:1422","NEWT:84030","NEWT:1423","NEWT:4932","NEWT:1205903","NEWT:3603","NEWT:2756","NEWT:2759","NEWT:38293","NEWT:1428","NEWT:339","NEWT:2751","NEWT:330","NEWT:139077","NEWT:391904","NEWT:12922","NEWT:316277","NEWT:163159","NEWT:1306413","NEWT:2813599","NEWT:518766","NEWT:2664373","NEWT:190802","NEWT:1213463","NEWT:7234","NEWT:9417","NEWT:1216979","NEWT:9895","NEWT:7237","NEWT:5059","NEWT:31631","NEWT:509924","NEWT:28450","NEWT:115104","NEWT:5061","NEWT:7240","NEWT:8570","NEWT:127569","NEWT:5062","NEWT:1408434","NEWT:1241935","NEWT:220668","NEWT:9407","NEWT:7227","NEWT:193079","NEWT:9402","NEWT:415540","NEWT:336722","NEWT:675060","NEWT:416870","NEWT:334542","NEWT:313625","NEWT:51240","NEWT:27592","NEWT:5052","NEWT:227289","NEWT:1985738","NEWT:7215","NEWT:196139","NEWT:9874","NEWT:5037","NEWT:651182","NEWT:2172103","NCBITaxon:59201","NEWT:7220","NEWT:1876254","NEWT:434271","NEWT:1915","NEWT:747679","NEWT:66712","NEWT:42752","NEWT:746360","NEWT:89764","NEWT:543734","NEWT:49390","NEWT:9870","NEWT:256648","NEWT:435590","NEWT:1902","NEWT:208526","NEWT:1908","NEWT:1169740","NEWT:217459","NEWT:216129","NEWT:1245012","NEWT:171250","NEWT:528135","NEWT:1637254","NCBITaxon:38727","NEWT:176946","NEWT:74790","NEWT:254472","NEWT:2829","NEWT:126279","NEWT:1228997","NEWT:1428006","NEWT:62335","NEWT:366581","NEWT:757417","NEWT:216599","NEWT:216595","NEWT:40559","NEWT:51208","NEWT:216597","NEWT:42736","NEWT:1607839","NEWT:1283300","NEWT:1218707","NEWT:1204551","NEWT:99586","NEWT:228577","NEWT:2060328","NEWT:48038","NEWT:392021","NEWT:436486","NEWT:51655","NEWT:1933298","NEWT:260707","NEWT:121845","NEWT:8595","NEWT:7263","NEWT:280754","NEWT:186763","NEWT:447135","NEWT:185431","NEWT:216591","NEWT:29760","NEWT:260704","NEWT:703612","NEWT:260705","NEWT:1202347","NEWT:1295743","NEWT:546805","NEWT:1505907","NEWT:1216981","NEWT:1249885","NEWT:1972855","NEWT:853","NEWT:7244","NEWT:1415167","NEWT:36036","NCBITaxon:662","NEWT:9430","NEWT:851","NEWT:71251","NEWT:208963","NEWT:208964","NEWT:181088","NEWT:48498","NEWT:669202","NEWT:1193806","NEWT:6306","NEWT:1250006","NEWT:4120","NEWT:820","NEWT:821","NEWT:37334","NCBITaxon:10357","NEWT:2040765","NCBITaxon:10358","NCBITaxon:10359","NEWT:1203258","NEWT:49317","NEWT:1899318","NEWT:696811","NEWT:9808","NEWT:839","NEWT:9803","NEWT:1097670","NEWT:92519","NEWT:943925","NEWT:4111","NEWT:37349","NEWT:4113","NEWT:837","NEWT:640459","NEWT:72568","NEWT:36015","NEWT:51641","NEWT:35168","NEWT:81866","NEWT:334147","NEWT:1262469","NEWT:555778","NEWT:277944","NEWT:48479","NEWT:61435","NEWT:627025","NEWT:1097677","NEWT:375939","NEWT:712938","NEWT:3258","NEWT:112018","NEWT:1906951","NEWT:101364","NEWT:52945","NEWT:4100","NEWT:1159556","NEWT:1076","NEWT:4102","NEWT:1028688","NEWT:4101","NEWT:6763","NEWT:803","NEWT:29722","NEWT:37319","NEWT:419947","NEWT:2847306","NEWT:184545","NEWT:468911","NEWT:13373","NEWT:266834","NEWT:817","NEWT:7604","NEWT:6756","NEWT:818","NEWT:135588","NEWT:1061","NEWT:4571","NEWT:29719","NEWT:6754","NEWT:452589","NEWT:509068","NEWT:2794344","NEWT:4577","NEWT:813","NEWT:1365218","NEWT:337677","NEWT:2086510","NEWT:1042986","NEWT:1911079","NEWT:69373","NEWT:427577","NEWT:264203","NEWT:1480154","NEWT:9858","NEWT:9859","NEWT:512623","NEWT:1274414","NEWT:2907833","NEWT:38626","NEWT:10298","NEWT:10299","NEWT:1087439","NEWT:9852","NEWT:9860","NEWT:660025","NEWT:61408","NEWT:1355477","NEWT:90371","NEWT:55194","NEWT:2907829","NEWT:4153","NEWT:11137","NEWT:278839","NEWT:59552","NEWT:35128","NEWT:39966","NEWT:7668","NEWT:2929635","NEWT:58228","NEWT:915099","NEWT:1143193","NEWT:681376","NEWT:687914","NEWT:1122233","NEWT:859313","NEWT:59540","NEWT:9838","NEWT:9839","NEWT:546414","NEWT:11167","NEWT:9835","NEWT:7650","NEWT:4146","NEWT:5478","NEWT:6326","NEWT:7656","NEWT:5476","NEWT:5477","NEWT:1882945","NEWT:746707","NEWT:299767","NEWT:28835","NEWT:1206781","NEWT:5480","NEWT:1274432","NEWT:222863","NEWT:2053667","NEWT:859321","NEWT:1274426","NEWT:9825","NEWT:1274423","NEWT:393765","NEWT:104421","NEWT:39946","NEWT:9823","NEWT:95602","NEWT:6313","NEWT:314539","NEWT:39947","NEWT:1274420","NEWT:859314","NEWT:578458","NEWT:60520"],"ORCID":["0000-0002-6131-7322"]}}