{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Raw":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC41_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC115_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS002.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC80_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC88_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA3_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC51_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC31_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC70_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC98_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA10_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC105_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC61_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS018.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC22_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC12_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC97_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC71_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC42_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC87_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS006.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC124_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC104_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC13_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC32_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC114_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC62_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC8_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC52_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS009.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC78_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC72_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC86_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC107_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS011.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC82_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA9_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC14_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA5_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC81_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC112_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC108_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC79_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC34_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC122_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA2_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC9_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC89_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC99_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC21_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC6_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC24_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC125_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC64_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC102_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC11_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC96_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC54_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC44_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS015.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC84_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC94_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC121_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC118_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS003.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC101_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC111_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC36_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC46_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC4_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC16_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC56_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC127_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS007.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC117_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC26_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC76_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC43_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC33_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC23_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC66_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS012.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC30_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC73_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC10_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC53_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC7_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC20_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC63_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC40_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC50_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC60_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS016.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA6_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC90_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC39_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC29_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS004.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC19_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC58_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC80_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC68_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC78_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC79_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC91_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC59_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC49_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC81_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC1_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS008.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC69_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC37_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC47_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC17_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC27_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC77_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC67_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS013.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC93_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC83_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC57_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC3_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC74_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC109_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC119_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS017.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS001.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC55_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC5_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC15_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC45_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC25_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC35_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC85_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA8_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC110_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS005.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC65_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC75_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC100_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA1_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC120_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC113_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC82_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC103_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC92_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC123_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC2_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS010.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC95_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC116_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC126_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA4_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/ASyS014.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC106_NE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC28_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC48_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC38_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/HC18_CE.raw.zip","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/RA7_CE.raw.zip"],"Mzid":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_IPMS_RA_and_asys.mzid","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p4.mzid","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p5.mzid","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p3.mzid","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p1.mzid","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p2.mzid"],"Mgf":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p1.DerivedFromSearch47b31f45-dce9-41a8-b103-1ebde849a95c.mgf","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_IPMS_RA_and_asys.DerivedFromSearch82f37e29-5333-4572-ad14-38cf66bc4035.mgf","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p2.DerivedFromSearch5e4eb4e2-1f7c-466f-9f95-016248e13f86.mgf","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p4.DerivedFromSearche79da36b-60d9-428b-9fa9-155b97845a8d.mgf","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p3.DerivedFromSearch12f9449a-5bff-49e5-892b-ca416d9811fa.mgf","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069628/PRIDE_RA_C_p5.DerivedFromSearche8048158-65d7-4b83-984e-7e826cd98b9b.mgf"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"submitter":["Birthe Michiels"],"software":["Not available"],"tissue":["Permanent Cell Line Cell","Cell Culture","Blood Serum"],"sample_protocol":["Immunoprecipitation (IP) was performed with sera at 1/20 dilution and 200 μg HeLa nuclear extract as described in (Vulsteke JB, et al. The TFIID complex is a new autoantibody target in systemic sclerosis. Ann Rheum Dis. 2025 Jun 19:S0003-4967(25)00968-9. doi: 10.1016/j.ard.2025.05.008. PMID: 40541453). For the IP-MS experiment, samples were resuspended in 10µl 0,1% formic acid (FA)/3% acetonitrile (ACN) and transferred to QuanRecovery vials (Waters). Five microliter of each sample was injected via the sample manager equipped with a 5µl sample loop, and separated on the M-class UPLC system (Waters) equipped with a nanoEase m/z Trap Column (300µm x 50mm, HSS T3, 100Å, 5µm, Waters) and a nanoEase m/z analytical column (300µm x 150mm, HSS T3, 100Å, 1.8µm, Waters). First, reverse elute trapping was performed using a gradient of 0,5% buffer B (0,1% FA in ACN) and 99,5% buffer A (0,1% FA in ULC/MS grade water (Biosolve)) for 3 minutes (15µl/min). Afterwards, column flow was set at 4µl/min with a gradient consisting of 5% buffer B for 5 minutes, 5-40% buffer B in 90 min, 40-99% buffer B in 5 min, 99% buffer B for 5 min and finally equilibration at 5% buffer B for 1 min. Total run time was 106 minutes. Column temperature was set to a constant 40°C. Mass spectrometry was performed using a Waters Xevo G3 QTOF equipped with a LockSpray dual electrospray ion source (Waters) in positive mode. The sample sprayer was set at a cone voltage of 30V and a capillary voltage of 2,8kV. The mass spectrometer was operated in MSe sensitivity mode with a typical resolving power of around 25 000. Mass shifts (due to changing temperatures) were corrected using 12pg/µl leucine enkephalin delivered through the reference sprayer of the source. The lock mass channel was sampled every minute. Accurate mass LCMS data was collected in alternating MS mode (6V collision energy) and MSe mode (15-40V ramping). Scan time was set at 0,5s. The detector was set to measure ions from 50 m/z to 2000 m/z."],"repository":["Pride"],"labhead":["Xavier Bossuyt"],"instrument_platform":[""],"labhead_affiliation":["Clinical and Diagnostic Immunology, Department Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium"],"submission_type":["COMPLETE"],"publication":["41963076 De Leeuw J, Michiels B, Derua R, Vulsteke JB, Dillaerts D, Cockx M, Verschueren P, Bossuyt X. Immunoprecipitation-mass spectrometry reveals known and novel (NatC, <i>BCS1L</i>) antinuclear antibodies in rheumatoid arthritis. RMD Open. 2026 12(2):e006642 10.1136/rmdopen-2025-006642"],"submitter_affiliation":["KU Leuven, Clinical and Diagnostic Immunology, Leuven, Belgium"],"labhead_mail":["xavier.bossuyt@uzleuven.be"],"technology_type":["Affinity purification coupled with mass spectrometry proteomics","Mass Spectrometry","Bottom-up proteomics","MSE"],"disease":["Rheumatoid Arthritis"],"submitter_keywords":["Autoantibodies","Rheumatoid arthritis","Anti nuclear antibodies","Mass spectrometry","Ipms"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD069628"],"quantification_method":[""],"modification":[""],"data_protocol":["Mass spectrometry data was compared to the Uniprot Homo sapiens reviewed database using ProteinLynx Global Server software 3.0.3 (PLGS, Waters). Low and elevated energy ion detection thresholds were set at 250 and 50 counts, respectively. Proteins were digested with trypsin and one missed cleavage was accepted. Peptide and fragment mass tolerances were calculated automatically by the software. Carbamidomethylation on cysteine was set as a fixed modification and deamidation of asparagine and glutamine and oxidation of methionine as variable modifications. False discovery rate was set at 4%. Minimally 2 and 5 fragment ions were needed to accept a peptide and protein identification, respectively. In addition, minimally 1 peptide match was needed to accept a protein identification. PLGS generates csv output files with for each protein identification a confidence color score (≥95% confidence = green; 50-95% confidence = yellow; <50% confidence = red), the number of matched peptides, the percentage sequence coverage and the sum of the intensities of the top 3 most intense peptides (Hi-3). The Hi-3 value can be used to relatively quantify identified proteins within one sample."],"omics_type":["Proteomics"],"species":["Homo Sapiens (human)"],"submitter_mail":["birthe.michiels@uzleuven.be"],"submitter_country":["Belgium"],"doi":["10.6019/PXD069628"],"pubmed_abstract":["

Objective

In a fraction of patients with rheumatoid arthritis (RA), antinuclear antibodies (ANA) are present. However, in most ANA-positive samples from patients with RA, no autoantigens can be identified. We aimed to characterise the ANA landscape in RA and to identify known and novel autoantigens using an unbiased immunoprecipitation-mass spectrometry (IP-MS) approach.

Methods

Sera from 51 ANA-positive, DMARD-naïve patients with RA were analysed by IP-MS using HeLa nuclear or cytoplasmic extracts (depending on the ANA staining pattern). Immunoprecipitated proteins were digested and identified using liquid chromatography coupled with MS. The prevalence of new or unfamiliar autoantigens was assessed in 43 healthy individuals and 88 ANA-positive individuals with a systemic rheumatic disease (systemic lupus erythematosus, Sjögren's disease, systemic sclerosis and idiopathic inflammatory myopathy).

Results

Autoantigens were detected in 10 of 51 RA samples (in 2/29 with titre 1/160, in 4/12 with titre 1/320 and in 4/10 with titre ≥1/640). Autoantigens identified included SSA-Ro60, SSB-La, Ku, fibrillarin, components of the mitochondrial multienzyme complex, protein argonaute 1, 2, 3 and T-cell restricted intracellular antigen 1 and TIA-1-related protein. Two novel autoantigens were discovered: the N-terminal acetyltransferase C (NatC) complex and mitochondrial chaperone BCS1. Neither NatC nor BCS1 was detected in healthy individuals or individuals with other systemic rheumatic diseases.

Conclusion

IP-MS revealed a diverse spectrum of autoantigens in patients with ANA-positive RA, including previously unreported targets. These findings expand our knowledge of RA-associated autoimmunity and highlight antibodies to NatC and BCS1 as potential novel biomarkers. Further studies are needed to assess their clinical relevance."],"pubmed_title":["Immunoprecipitation-mass spectrometry reveals known and novel (NatC, <i>BCS1L</i>) antinuclear antibodies in rheumatoid arthritis."],"pubmed_authors":["De Leeuw Jonas J, Michiels Birthe B, Derua Rita R, Vulsteke Jean-Baptiste JB, Dillaerts Doreen D, Cockx Maaike M, Verschueren Patrick P, Bossuyt Xavier X"],"additional_accession":[]},"is_claimable":false,"name":"Novel autoantibodies in rheumatoid arthritis using immunoprecipitation coupled with mass spectrometric analysis.","description":"Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic joint inflammation resulting in progressive cartilage and bone destruction. In two thirds of early RA patients, rheumatoid factor (RF) and/or anti-citrullinated protein antibodies (ACPAs) are present. While these antibodies are well-established markers of RA, antinuclear antibodies (ANAs) are found in 25% - 77% of RA patients, a prevalence that is significantly higher than the prevalence of ANA in the general population1,2. However, in most ANA positive samples from RA patients no specific autoantigen can be identified. In this study, we set out to expand our knowledge on the ANA landscape in RA by applying a recently described 3–5 unbiased immunoprecipitation-mass spectrometry (IP-MS) approach, which allows identification of known and unknown autoantigens. IP-MS was performed on 55 ANA-positive (titer ≤1/160, nuclear or cytoplasmatic staining pattern) samples from newly diagnosed, DMARD-naïve RA patients, selected out of 191 newly diagnosed RA patients included in three differentcohorts. Autoantigens were identified in 10 of the 55 samples. IP-MS identified antibodies to SSA and SSB (#1), Ku (#2), fibrillarin (#3) and the mitochondrial dihydrolipoyllysine-residue acetyltransferase and pyruvate dehydrogenase complex (#4, 7, 10), protein argonaute (AGO) 1, 2 and 3 (#5,6), dihydrolipoamide branched chain transacylase E2 (DBT) (#7), T-cell intracytoplasmic antigen 1(TIA1)/Nucleolysin TIAR (TIAL1) (#8), the N-terminal acetyltransferase C (NatC) complex (#9) and the mitochondrial chaperone protein BCS1 (#10). Anti-SSA and anti-SSB antibodies are most commonly detected in primary Sjögren’s syndrome (pSjS) and systemic lupus erythematosus (SLE), but they can also occur, less frequently, in other connective tissue diseases, including rheumatoid arthritis (RA) and systemic sclerosis (SSc), and only rarely in idiopathic inflammatory myopathies (IIM) or antisynthetase syndromes (ASyS). Anti-Ku antibodies are classically associated with overlap syndromes and may be present in patients with systemic sclerosis, SLE, or IIM. Anti-fibrillarin antibodies exhibit high specificity for systemic sclerosis (SSc), particularly the diffuse cutaneous subtype, and are only infrequently observed in overlap syndromes. Considering the high specificity for anti-fibrillarin antibody positivity with SSc, a careful monitoring during routine follow-up was provided. At present, however, the patient does not manifest any clinical features indicative of SSc. Antibodies to AGO2 (anti-Su) are found in various rheumatic and neurological diseases and in apparently healthy individuals at low prevalence 6,7. Similarly, antibodies to TIA1/TIAL1 have been described in several rheumatic diseases (SLE, SSc and RA) and in healthy individuals8. DBT has previously been identified as an antigenic component of anti-mitochondrial M2 antibodies and is predominantly associated with SSc. The N-terminal acetyltransferase C (NatC) complex was identified in sample (#9) with a cytoplasmic reticular staining. The NatC complex is engaged in N-terminal protein acetylation (a post-translational modification) and regulates protein stability and function. BCS1L was found in a sample together with DLAT, PDHA1 and PDHB, subunits known as representing antimitochondrial antibodies (#10). BCS1 is a mitochondrial chaperone protein and a requisite for respiratory chain complex III assembly. Autoantibodies against mitochondrial proteins have been described in RA9, but BCS1L has not yet been identified as mitochondrial autoantigen in the literature. Antibodies to the NatC complex and to BCS1L were not found in samples from 43 healthy controls (analyzed in 82 runs with cytoplasmatic extract and 127 runs with nuclear extract as protein source for IP). These were not present in samples from 102 inflammatory controls, including individuals with anti-synthetase syndrome (n=14 with 18 separate MS runs), IIM (n=12), pSjS (n=12), SLE (n=36) and SSc (n=28). In conclusion, IP-MS identified autoantigens in 10/55 ANA positive samples. A multitude of autoantigenic targets was revealed, including novel autoantigens. Further studies are needed to explore the potential clinical relevance and pathogenicity of autoantibodies against the NatC complex and BCS1.","dates":{"publication":"2026-04-27","submission":"2025-10-19"},"accession":"PXD069628","cross_references":{"TAXONOMY":["NEWT:330879","NEWT:377960","NEWT:2042546","NEWT:259447","NEWT:295546","NCBITaxon:1280","NEWT:112503","NEWT:1129","NEWT:309807","NEWT:59753","NEWT:89184","NEWT:309800","NEWT:281395","NEWT:1211601","NEWT:876138","NEWT:44271","NEWT:193516","NEWT:1073882","NEWT:6819","NEWT:475174","NEWT:1117","NEWT:498257","NEWT:10036","NEWT:1590","NEWT:10034","NEWT:661410","NEWT:638632","NEWT:224326","NEWT:376619","NCBITaxon:79857","NEWT:1096976","NEWT:3765","NEWT:1589","NEWT:135622","NEWT:35786","NEWT:1580","NEWT:399784","NEWT:96731","NEWT:656064","NEWT:36630","NEWT:383379","NEWT:418106","NCBITaxon:12721","NEWT:10029","NEWT:913645","NEWT:641809","NEWT:1392488","NEWT:38815","NEWT:317447","NEWT:4688","NEWT:7719","NEWT:868565","NEWT:135674","NEWT:79329","NEWT:30069","NEWT:12637","NEWT:59729","NEWT:2164133","NEWT:295105","NEWT:108061","NEWT:60711","NEWT:224308","NEWT:3347","NEWT:160621","NEWT:212790","NEWT:110368","NEWT:1310161","NEWT:77133","NEWT:145481","NEWT:1310165","NEWT:29058","NCBITaxon:79824","NEWT:410658","NEWT:44688","NEWT:44689","NEWT:498211","NEWT:347256","NEWT:5518","NEWT:398007","NEWT:1527468","NEWT:498217","NEWT:498216","NEWT:11320","NEWT:246196","NEWT:246197","NEWT:145458","NEWT:44685","NEWT:161934","NEWT:1148","NEWT:5508","NEWT:3329","NEWT:419481","NEWT:5507","NEWT:410661","NEWT:1140","NCBITaxon:2157","NEWT:1143","NEWT:1287689","NEWT:1094343","NEWT:1462472","NEWT:1336795","NEWT:644042","NEWT:1182590","NEWT:3712","NEWT:3711","NEWT:3714","NEWT:2065263","NEWT:177437","NEWT:10418","NEWT:118698","NEWT:1616117","NEWT:118696","NEWT:34865","NEWT:52283","NEWT:284812","NEWT:8175","NEWT:43330","NEWT:1603293","NEWT:44664","NEWT:43346","NEWT:3702","NEWT:1245466","NEWT:3704","NEWT:244366","NEWT:1246791","NEWT:118694","NEWT:2850","NEWT:118691","NEWT:34871","NEWT:33548","NEWT:52299","NEWT:8165","NEWT:96794","NEWT:3708","NEWT:332648","NEWT:44670","NEWT:536231","NEWT:376219","NEWT:219813","NEWT:1510","NEWT:460519","NEWT:1515","NEWT:572307","NEWT:1432138","NEWT:1424507","NEWT:1194599","NEWT:272844","NEWT:1348799","NEWT:33995","NEWT:55784","NEWT:1303443","NEWT:485","NEWT:56636","NEWT:2853422","NEWT:1679718","NEWT:480","NEWT:67767","NEWT:46835","NEWT:109757","NEWT:582580","NEWT:1185650","NEWT:216778","NEWT:1502","NEWT:128017","NEWT:376686","NEWT:95486","NEWT:1883446","NEWT:1233435","NEWT:109760","NEWT:29031","NEWT:317047","NEWT:491","NEWT:460523","NEWT:235443","NEWT:1757312","NEWT:85694","NEWT:108458","NEWT:5936","NEWT:320637","NEWT:1078283","NEWT:3750","NEWT:983964","NEWT:11706","NEWT:32644","NEWT:527796","NEWT:499175","NEWT:109779","NEWT:3745","NEWT:1715989","NCBITaxon:4751","NEWT:3747","NEWT:1116234","NEWT:1255228","NEWT:410289","NEWT:373153","NEWT:1640278","NEWT:472","NEWT:940825","NEWT:529507","NEWT:1071661","NEWT:470","NEWT:5911","NEWT:748693","NCBITaxon:50557","NEWT:39251","NEWT:29491","NEWT:101841","NEWT:446","NEWT:153481","NEWT:2014887","NEWT:33952","NEWT:445","NEWT:153009","NEWT:261756","NEWT:166313","NEWT:63366","NEWT:63367","NEWT:215402","NEWT:1547","NEWT:906914","NEWT:9031","NEWT:27292","NEWT:1050722","NEWT:108931","NEWT:1293497","NEWT:1055524","NEWT:150475","NEWT:267872","NEWT:381666","NEWT:172269","NEWT:9534","NEWT:5180","NEWT:256737","NEWT:9541","NEWT:8694","NEWT:33936","NEWT:8692","NEWT:2903","NEWT:185579","NEWT:13076","NEWT:1006581","NEWT:8673","NEWT:33940","NEWT:550","NEWT:554","NEWT:451516","NEWT:552","NEWT:1325291","NEWT:36185","NEWT:1054211","NEWT:1225786","NEWT:771870","NEWT:51338","NEWT:575412","NEWT:28112","NEWT:6493","NEWT:10832","NEWT:6494","NEWT:6491","NEWT:507601","NEWT:520","NEWT:186441","NEWT:643680","NEWT:214092","NCBITaxon:6157","NEWT:13095","NEWT:162425","NEWT:104105","NEWT:216257","NEWT:9986","NEWT:8654","NEWT:8658","NEWT:1268063","NEWT:8657","NEWT:9983","NEWT:8655","NEWT:5147","NEWT:28108","NEWT:28104","NEWT:407821","NEWT:568703","NCBITaxon:2","NEWT:568708","NEWT:986","NEWT:52641","NEWT:28532","NEWT:353152","NEWT:298176","NEWT:227377","NEWT:1774284","NEWT:40674","NEWT:1194669","NEWT:443906","NEWT:519","NEWT:2510939","NEWT:7394","NEWT:6063","NEWT:1328388","NEWT:105487","NEWT:1548728","NEWT:667127","NEWT:9557","NEWT:377586","NEWT:300641","NEWT:39655","NEWT:9554","NEWT:475932","NEWT:256318","NEWT:206411","NCBITaxon:6191","NEWT:229533","NEWT:214053","NEWT:28550","NEWT:80863","NEWT:90675","NEWT:52638","NEWT:57075","NEWT:1666905","NEWT:8697","NEWT:8695","NEWT:884204","NEWT:1123869","NEWT:9544","NEWT:9545","NEWT:979","NEWT:7370","NEWT:83906","NEWT:99488","NEWT:1134506","NEWT:255470","NEWT:38783","NEWT:6426","NEWT:33090","NEWT:9935","NEWT:287889","NEWT:305959","NEWT:13489","NEWT:29829","NEWT:92867","NEWT:92866","NCBITaxon:3055","NEWT:51750","NEWT:202950","NEWT:441772","NEWT:48106","NEWT:295027","NCBITaxon:11320","NEWT:632957","NEWT:9925","NCBITaxon:9606","NEWT:90690","NEWT:1436183","NEWT:300273","NEWT:4232","NEWT:416348","NEWT:11298","NEWT:4236","NEWT:7742","NEWT:196627","NEWT:200308","NEWT:200302","NEWT:870435","NEWT:9913","NEWT:9915","NEWT:105841","NEWT:2666255","NEWT:999810","NCBITaxon:5693","NEWT:28995","NEWT:2144189","NEWT:1392998","NEWT:380394","NEWT:226900","NEWT:1266738","NEWT:231490","NEWT:244704","NEWT:7725","NEWT:9909","NEWT:9902","NEWT:430615","NEWT:72664","NEWT:563041","NEWT:36599","NEWT:41949","NEWT:249161","NEWT:326423","NEWT:452467","NEWT:198822","NEWT:36111","NEWT:326424","NEWT:1678078","NEWT:749906","NEWT:418985","NEWT:749907","NEWT:150847","NEWT:29835","NEWT:431947","NEWT:69014","NEWT:142809","NEWT:302409","NEWT:130821","NEWT:27606","NEWT:1519788","NEWT:59202","NEWT:9975","NEWT:8643","NEWT:1801631","NEWT:1159899","NEWT:502780","NEWT:860688","NEWT:13443","NEWT:35231","NEWT:8644","NEWT:1125630","NEWT:5141","NEWT:46360","NCBITaxon:3044782","NEWT:202914","NEWT:160235","NEWT:502779","NEWT:297246","NEWT:8639","NEWT:1928434","NEWT:8637","NEWT:1114970","NEWT:2096","NEWT:285272","NEWT:192222","NEWT:9963","NEWT:1617910","NEWT:637379","NEWT:12107","NEWT:2785785","NEWT:411901","NEWT:1298722","NEWT:8626","NEWT:186801","NEWT:105884","NCBITaxon:9615","NEWT:8621","NEWT:58334","NEWT:58331","NEWT:8624","NEWT:5116","NEWT:8623","NEWT:5597","NEWT:194439","NCBITaxon:12637","NEWT:38727","NEWT:218496","NEWT:8618","NEWT:70448","NEWT:300251","NEWT:9940","NEWT:1247190","NEWT:118702","NEWT:118700","NEWT:2126719","NEWT:118705","NEWT:6945","NEWT:2104","NEWT:2627626","NEWT:6943","NEWT:157546","NEWT:870726","NEWT:91374","NEWT:91373","NEWT:474186","NEWT:54195","NEWT:10136","NEWT:38942","NEWT:4762","NEWT:6941","NEWT:307972","NEWT:2102","NEWT:34","NEWT:38949","NEWT:1299114","NEWT:1590841","NEWT:60876","NEWT:1276473","NEWT:2282334","NEWT:1247","NEWT:70667","NEWT:869728","NEWT:6938","NCBITaxon:39054","NEWT:1736309","NEWT:4751","NEWT:194963","NEWT:15819","NEWT:109461","NEWT:1085956","NEWT:6928","NEWT:1239","NEWT:36745","NEWT:400727","NEWT:272563","NEWT:11009","NEWT:913998","NEWT:68335","NEWT:3885","NEWT:3888","NEWT:649408","NEWT:6915","NEWT:101510","NEWT:3880","NEWT:272559","NEWT:216816","NEWT:153151","NEWT:2681598","NEWT:515849","NEWT:10141","NEWT:95767","NEWT:466585","NEWT:1000589","NEWT:85963","NEWT:85962","NEWT:42197","NEWT:143361","NEWT:5656","NEWT:1299","NEWT:316407","NEWT:1794699","NCBITaxon:183674","NEWT:167387","NEWT:84645","NEWT:1123294","NEWT:6978","NEWT:209285","NEWT:211586","NEWT:747078","NEWT:1282","NEWT:1283","NEWT:1000561","NEWT:2133","NEWT:294381","NEWT:197","NEWT:1390363","NEWT:35889","NEWT:406072","NEWT:29176","NEWT:201319","NEWT:1073569","NEWT:4787","NEWT:751585","NEWT:113549","NEWT:180788","NEWT:159749","NEWT:1270","NEWT:388919","NEWT:130081","NEWT:4784","NEWT:360106","NEWT:4785","NEWT:360104","NEWT:10117","NEWT:401614","NEWT:10116","NEWT:1280","NEWT:83333","NEWT:83334","NEWT:242718","NEWT:83332","NEWT:242715","NEWT:100226","NEWT:1263","NEWT:176299","NEWT:54126","NEWT:75058","NEWT:13616","NEWT:13613","NEWT:296543","NEWT:173","NEWT:316435","NEWT:42157","NEWT:1034304","NEWT:105023","NEWT:866628","NEWT:535026","NEWT:749200","NEWT:1036967","NEWT:266264","NEWT:272943","NEWT:145953","NEWT:257309","NEWT:203124","NEWT:230741","NEWT:8296","NEWT:117008","NEWT:167748","NEWT:1163394","NEWT:645463","NEWT:749696","NEWT:990119","NEWT:77216","NEWT:1111708","NEWT:998086","NEWT:493760","NEWT:93163","NEWT:257313","NEWT:3827","NEWT:400772","NEWT:257310","NEWT:128161","NEWT:930945","NEWT:332521","NEWT:106350","NEWT:269796","NEWT:54571","NEWT:412754","NEWT:9598","NEWT:1423772","NEWT:51056","NEWT:35815","NEWT:53232","NEWT:1235987","NCBITaxon:2509511","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:432359","NEWT:9103","NEWT:715877","NEWT:862238","NEWT:29159","NEWT:1155748","NEWT:9593","NEWT:8260","NEWT:9595","NEWT:2665958","NEWT:246200","NEWT:6087","NEWT:145943","NEWT:595536","NEWT:1134822","NEWT:240906","NEWT:89920","NEWT:29158","NEWT:44744","NEWT:3879","NEWT:1328881","NEWT:411483","NEWT:76336","NEWT:3870","NEWT:884019","NEWT:411490","NEWT:169963","NEWT:587","NEWT:588","NEWT:585","NEWT:746128","NEWT:212035","NCBITaxon:1653394","NEWT:1205","NEWT:214693","NEWT:45609","NEWT:9172","NEWT:2770979","NEWT:112","NEWT:1277351","NEWT:1594786","NEWT:3854","NEWT:411469","NEWT:1679","NEWT:333849","NEWT:214684","NEWT:1758543","NEWT:569","NEWT:411464","NEWT:411460","NEWT:1328426","NEWT:562","NEWT:411470","NEWT:2094720","NCBITaxon:2697049","NEWT:294750","NEWT:1663","NEWT:62141","NEWT:3848","NEWT:3847","NEWT:198466","NEWT:327159","NEWT:1045010","NEWT:178876","NEWT:1660","NEWT:327160","NEWT:573","NEWT:571","NEWT:142479","NEWT:29595","NEWT:241368","NEWT:42528","NEWT:1969471","NEWT:9657","NEWT:150596","NEWT:30540","NEWT:264483","NEWT:439733","NEWT:76777","NEWT:30545","NEWT:76773","NEWT:1121114","NEWT:663","NEWT:1081927","NEWT:1238993","NEWT:67825","NEWT:187878","NEWT:1504802","NEWT:1821314","NEWT:1249668","NEWT:426430","NEWT:37181","NEWT:929439","NEWT:9646","NEWT:5286","NEWT:7469","NEWT:9644","NEWT:426428","NEWT:63459","NEWT:2652157","NEWT:2652158","NEWT:40353","NEWT:40324","NEWT:588858","NEWT:9638","NEWT:9639","NCBITaxon:11071","NEWT:693977","NEWT:242231","NEWT:197221","NEWT:528354","NEWT:51453","NEWT:1279085","NEWT:28231","NEWT:7460","NEWT:504457","NEWT:392227","NEWT:1004024","NEWT:103372","NEWT:862751","NEWT:470150","NEWT:9627","NEWT:1009714","NEWT:73239","NEWT:38018","NEWT:226186","NEWT:381518","NEWT:30532","NEWT:8782","NEWT:115357","NEWT:5270","NEWT:63436","NEWT:63433","NEWT:13164","NEWT:1601067","NEWT:129554","NEWT:40302","NEWT:1249615","NEWT:2664179","NEWT:623","NEWT:7038","NEWT:6192","NCBITaxon:1639","NEWT:3915","NEWT:1581419","NEWT:405534","NEWT:9689","NEWT:8355","NEWT:633","NEWT:9685","NEWT:7029","NEWT:9688","NEWT:6182","NEWT:9694","NEWT:604162","NEWT:186990","NEWT:8364","NEWT:632","NEWT:6183","NEWT:1423800","NEWT:630","NCBITaxon:11019","NEWT:334747","NEWT:3907","NEWT:61235","NEWT:343691","NEWT:1457551","NEWT:15368","NEWT:13180","NEWT:1491466","NEWT:340188","NEWT:170187","NEWT:1755437","NEWT:1042399","NEWT:1719","NEWT:692672","NEWT:2697049","NEWT:1109443","NEWT:267566","NEWT:58042","NEWT:7004","NEWT:9669","NCBITaxon:6073","NEWT:1089448","NEWT:1329349","NEWT:1089444","NEWT:607699","NEWT:6161","NEWT:206542","NEWT:27337","NEWT:575584","NEWT:186536","NEWT:257771","NEWT:185687","NEWT:3039","NEWT:2706014","NEWT:8727","NEWT:8726","NEWT:10090","NEWT:8729","NEWT:8720","NEWT:5693","NEWT:2186","NEWT:8724","NEWT:8723","NEWT:283166","NEWT:8722","NEWT:5697","NEWT:6544","NEWT:218192","NCBITaxon:10114","NEWT:1685931","NEWT:242619","NEWT:557722","NEWT:160791","NEWT:1353008","NEWT:34073","NEWT:402042","NEWT:373995","NEWT:8715","NEWT:5689","NEWT:544404","NEWT:287535","NEWT:2173","NEWT:2175","NEWT:1232801","NEWT:5691","NEWT:61674","NEWT:2072580","NEWT:669465","NEWT:1117957","NEWT:6526","NEWT:8709","NEWT:8707","NEWT:1306274","NEWT:5671","NEWT:13146","NEWT:8700","NEWT:1692259","NEWT:1486917","NEWT:1843183","NEWT:5661","NEWT:157592","NEWT:51866","NEWT:58002","NEWT:13136","NEWT:5660","NEWT:5665","NEWT:1179777","NEWT:6997","NEWT:5666","NEWT:1746091","NEWT:5664","NEWT:2157","NEWT:70125","NEWT:146479","NEWT:1843192","NEWT:206189","NEWT:295139","NEWT:6108","NEWT:1720309","NEWT:9612","NEWT:1286640","NEWT:38865","NEWT:3077","NEWT:9615","NEWT:6100","NEWT:713604","NEWT:3076","NEWT:112273","NEWT:33178","NEWT:1147787","NEWT:1440772","NEWT:8759","NEWT:9605","NEWT:9606","NEWT:27717","NEWT:641501","NEWT:9601","NEWT:8752","NEWT:8756","NEWT:74940","NEWT:13551","NEWT:10041","NEWT:9600","NEWT:1450511","NEWT:439375","NEWT:890382","NEWT:174934","NEWT:179392","NEWT:2823525","NEWT:115778","NEWT:1538186","NEWT:227086","NEWT:3055","NEWT:195883","NEWT:1450520","NEWT:1042311","NEWT:1123352","NEWT:741258","NEWT:82525","NEWT:29920","NEWT:235909","NEWT:47311","NCBITaxon:813","NEWT:127785","NEWT:708248","NEWT:104660","NEWT:652611","NEWT:94993","NEWT:8732","NEWT:763037","NEWT:3555","NEWT:71647","NEWT:260201","NEWT:35554","NCBITaxon:12939","NEWT:32046","NEWT:2222","NEWT:544496","NEWT:59529","NEWT:32049","NEWT:1392696","NEWT:45351","NEWT:445974","NEWT:43179","NEWT:180454","NEWT:5722","NEWT:7901","NEWT:32051","NEWT:10245","NEWT:55153","NEWT:351581","NEWT:43186","NEWT:428146","NEWT:498019","NEWT:1351","NEWT:1438992","NEWT:1352","NEWT:32025","NEWT:2649997","NEWT:1147161","NEWT:43150","NEWT:263737","NEWT:1333499","NEWT:5702","NEWT:95648","NEWT:1349","NEWT:1401257","NEWT:44491","NEWT:27933","NEWT:556484","NEWT:7955","NEWT:7959","NEWT:2261","NEWT:94323","NEWT:31156","NEWT:3112","NEWT:8801","NEWT:4442","NEWT:192875","NEWT:1214916","NEWT:284218","NEWT:1316931","NEWT:511145","NEWT:213401","NEWT:931281","NEWT:4432","NEWT:5762","NEWT:5763","NEWT:118099","NEWT:658457","NEWT:35525","NEWT:295358","NEWT:2246","NEWT:2652724","NEWT:5757","NEWT:68415","NCBITaxon:4563","NEWT:5755","NEWT:44447","NEWT:752555","NEWT:39097","NEWT:347495","NEWT:5759","NEWT:1736231","NEWT:1392","NEWT:35974","NEWT:2242","NEWT:1396","NEWT:286","NEWT:391619","NEWT:287","NEWT:1367269","NEWT:633149","NEWT:1149786","NEWT:10239","NEWT:68895","NEWT:3567","NEWT:4897","NEWT:5744","NEWT:72901","NEWT:213863","NEWT:55571","NEWT:35500","NEWT:2041044","NEWT:3562","NEWT:4896","NEWT:1262161","NEWT:1314781","NEWT:1390","NEWT:11557","NEWT:294","NEWT:1773","NEWT:82380","NEWT:935293","NEWT:375146","NEWT:471876","NEWT:1772","NEWT:263","NEWT:11988","NEWT:400667","NEWT:408169","NEWT:47946","NEWT:1764","NEWT:39087","NEWT:413996","NEWT:243277","NEWT:7067","NEWT:586722","NEWT:243273","NEWT:274","NEWT:7070","NCBITaxon:287","NEWT:408172","NEWT:408170","NEWT:1436733","NEWT:42229","NEWT:1247411","NEWT:9238","NEWT:13708","NEWT:333760","NEWT:34607","NEWT:1513458","NEWT:1233681","NEWT:2212966","NCBITaxon:40559","NEWT:506599","NEWT:81077","NEWT:84588","NEWT:1279007","NEWT:85433","NEWT:65349","NEWT:300852","NEWT:564933","NEWT:508771","NEWT:1121405","NEWT:253","NCBITaxon:2759","NEWT:93061","NEWT:93062","NEWT:1284813","NEWT:34613","NCBITaxon:4932","NEWT:1748","NEWT:108673","NEWT:943274","NEWT:644223","NEWT:1263082","NEWT:272623","NEWT:1288389","NEWT:272624","NEWT:886559","NEWT:55521","NEWT:118499","NEWT:1130798","NEWT:55529","NEWT:256136","NEWT:476272","NEWT:195051","NEWT:3988","NEWT:561007","NEWT:649908","NEWT:147828","NEWT:3983","NEWT:203696","NEWT:352472","NEWT:505235","NEWT:55518","NEWT:97441","NEWT:1154756","NEWT:1202531","NEWT:1202532","NEWT:360094","NEWT:1408223","NEWT:1408224","NEWT:41364","NEWT:1311","NEWT:1313","NEWT:1218097","NEWT:1080348","NEWT:1314","NEWT:39491","NEWT:212142","NCBITaxon:5811","NEWT:1499105","NEWT:192812","NEWT:408188","NEWT:571256","NEWT:40483","NEWT:688333","NCBITaxon:548681","NEWT:318586","NCBITaxon:5820","NEWT:272634","NEWT:198107","NEWT:1979401","NEWT:155892","NEWT:691","NEWT:28384","NEWT:167879","NEWT:1872122","NEWT:1308","NEWT:7091","NEWT:1034015","NEWT:760568","NEWT:141262","NEWT:1813","NEWT:4087","NEWT:9778","NEWT:306","NEWT:303","NEWT:97477","NEWT:7111","NEWT:113287","NEWT:347515","NEWT:6269","NEWT:4090","NEWT:4092","NEWT:4093","NEWT:4096","NEWT:208895","NEWT:941442","NEWT:536088","NEWT:311553","NEWT:1226408","NEWT:43984","NEWT:7108","NEWT:40479","NEWT:43989","NEWT:4076","NEWT:142615","NEWT:317","NEWT:885319","NEWT:293939","NEWT:497515","NEWT:885318","NEWT:39488","NEWT:4081","NEWT:273068","NEWT:1316582","NEWT:227166","NEWT:98334","NEWT:231663","NEWT:4084","NEWT:63577","NEWT:7574","NEWT:1715256","NEWT:30640","NEWT:348824","NEWT:929793","NEWT:1507179","NEWT:29204","NEWT:114155","NEWT:4072","NEWT:1337056","NEWT:634955","NEWT:6239","NEWT:102169","NEWT:208893","NEWT:4054","NEWT:98351","NEWT:1263854","NEWT:118503","NEWT:2059687","NEWT:160488","NEWT:63558","NEWT:207559","NEWT:107401","NEWT:85057","NEWT:985076","NEWT:1215323","NEWT:7159","NEWT:267671","NEWT:7160","NEWT:8496","NEWT:7165","NEWT:1257118","NEWT:1387333","NEWT:8010","NEWT:1315282","NEWT:27448","NEWT:235612","NEWT:243230","NEWT:936053","NEWT:1080772","NEWT:8479","NEWT:8485","NEWT:119856","NEWT:119857","NEWT:49240","NCBITaxon:3707","NEWT:391295","NEWT:1170828","NEWT:93934","NEWT:9315","NEWT:6289","NEWT:6287","NEWT:38569","NEWT:727","NEWT:9796","NEWT:725","NEWT:7137","NEWT:469008","NEWT:9321","NEWT:596153","NEWT:1836","NCBITaxon:10298","NEWT:105231","NEWT:104385","NEWT:74649","NEWT:1037924","NEWT:206672","NEWT:9788","NEWT:4097","NEWT:9305","NEWT:436113","NEWT:71139","NEWT:9785","NEWT:6279","NEWT:1195774","NEWT:9793","NCBITaxon:780","NCBITaxon:1502","NEWT:6282","NEWT:27457","NEWT:4006","NEWT:1872635","NCBITaxon:5476","NEWT:113208","NEWT:6669","NEWT:1215360","NEWT:89453","NEWT:1114301","NEWT:51515","NEWT:13249","NEWT:588596","NEWT:51511","NEWT:8845","NEWT:7998","NEWT:419610","NEWT:5334","NEWT:382352","NEWT:413071","NCBITaxon:10239","NEWT:332097","NCBITaxon:714","NEWT:11051","NEWT:89462","NEWT:452680","NEWT:8839","NEWT:2758385","NEWT:171101","NEWT:714","NEWT:5322","NEWT:421932","NEWT:312017","NEWT:576131","NEWT:553470","NEWT:284593","NEWT:100392","NEWT:655819","NEWT:5315","NEWT:11082","NEWT:265872","NEWT:59463","NEWT:337330","NEWT:2285","NEWT:2287","NEWT:6645","NEWT:251229","NEWT:981087","NEWT:180066","NEWT:46147","NCBITaxon:6312","NEWT:11072","NEWT:174633","NEWT:1416333","NEWT:35024","NEWT:135461","NEWT:7962","NEWT:99287","NEWT:1817808","NEWT:145262","NEWT:145263","NEWT:694569","NEWT:9739","NEWT:111069","NEWT:1379791","NEWT:51953","NEWT:57267","NEWT:3197","NEWT:13686","NEWT:2750454","NEWT:264561","NEWT:1402135","NEWT:36329","NEWT:62615","NEWT:1555636","NEWT:1333550","NEWT:72407","NEWT:352914","NEWT:349741","NEWT:409859","NEWT:157295","NEWT:1263871","NEWT:509169","NEWT:6210","NEWT:9721","NEWT:137221","NEWT:565050","NEWT:409860","NEWT:92683","NEWT:1298968","NEWT:5353","NEWT:1034809","NEWT:452659","NEWT:1193501","NEWT:51930","NEWT:6689","NEWT:194675","NEWT:298315","NEWT:392547","NEWT:46105","NEWT:104782","NEWT:10181","NEWT:9704","NEWT:1049056","NEWT:698936","NEWT:1009874","NEWT:4498","NEWT:8855","NEWT:11034","NEWT:3168","NEWT:10185","NEWT:78168","NEWT:521001","NEWT:192011","NEWT:340912","NEWT:1813887","NEWT:224911","NCBITaxon:11552","NEWT:334249","NEWT:184922","NEWT:6703","NEWT:2","NEWT:35670","NEWT:511693","NEWT:4528","NEWT:35677","NEWT:1493","NEWT:10376","NEWT:1496","NEWT:3674","NEWT:5851","NEWT:660469","NEWT:935568","NEWT:1365186","NEWT:1813019","NEWT:45474","NEWT:48500","NEWT:4513","NEWT:5843","NEWT:1117645","NCBITaxon:10407","NEWT:1000364","NEWT:10360","NEWT:1403536","NEWT:1427524","NEWT:47664","NEWT:260799","NEWT:317548","NEWT:2323","NEWT:3654","NEWT:237561","NEWT:5833","NEWT:3659","NEWT:59620","NEWT:499229","NEWT:31298","NEWT:1204162","NEWT:82688","NEWT:5823","NEWT:5821","NEWT:44139","NCBITaxon:4895","NCBITaxon:4896","NEWT:675512","NEWT:315393","NEWT:3649","NEWT:28903","NEWT:28909","NEWT:3641","NEWT:79698","NCBITaxon:5796","NEWT:12993","NEWT:398580","NEWT:4565","NEWT:1264690","NEWT:515619","NEWT:170721","NEWT:34305","NEWT:77020","NEWT:83558","NEWT:44586","NEWT:626528","NEWT:139927","NEWT:4558","NEWT:5888","NEWT:431241","NEWT:4550","NEWT:4555","NEWT:144030","NEWT:288705","NEWT:392815","NEWT:3217","NEWT:4547","NEWT:3218","NEWT:556182","NEWT:374990","NEWT:1432059","NEWT:154293","NEWT:156471","NEWT:3694","NEWT:156476","NEWT:4543","NEWT:210007","NEWT:1857017","NEWT:10359","NEWT:1358430","NEWT:10358","NEWT:1735272","NEWT:109298","NEWT:317513","NEWT:580240","NEWT:294128","NEWT:11676","NEWT:1917265","NEWT:55210","NEWT:718308","NEWT:4530","NEWT:5861","NEWT:1895","NEWT:2558360","NEWT:64152","NEWT:4924","NEWT:2748","NEWT:8049","NEWT:33892","NEWT:99931","NEWT:9378","NEWT:990346","NEWT:100816","NEWT:192952","NEWT:1640346","NCBITaxon:1313","NEWT:29385","NEWT:44544","NEWT:529818","NEWT:400540","NEWT:382","NEWT:4911","NEWT:152480","NEWT:129249","NEWT:1888","NEWT:1077286","NEWT:943129","NEWT:1221594","NEWT:1524260","NEWT:227321","NEWT:281677","NEWT:106590","NEWT:260710","NEWT:4918","NEWT:1162296","NEWT:106592","NEWT:596645","NEWT:46704","NEWT:42345","NEWT:482428","NEWT:1187947","NEWT:4903","NEWT:10312","NCBITaxon:1773","NEWT:190304","NEWT:1432499","NEWT:8030","NEWT:246437","NEWT:36909","NEWT:4909","NEWT:1279365","NEWT:759272","NEWT:1182263","NEWT:943146","NEWT:646532","NEWT:2711","NEWT:537457","NEWT:479249","NEWT:33889","NEWT:10306","NEWT:8022","NEWT:42353","NEWT:593117","NEWT:3633","NEWT:319761","NEWT:3635","NEWT:5811","NEWT:86662","NEWT:180923","NEWT:1451","NEWT:198215","NEWT:200298","NEWT:12916","NEWT:1400870","NEWT:343","NEWT:84005","NEWT:225117","NEWT:8090","NEWT:5801","NEWT:43665","NEWT:5802","NEWT:4952","NEWT:1266464","NEWT:367830","NEWT:1093790","NEWT:178616","NEWT:95145","NEWT:358","NEWT:375451","NEWT:54788","NEWT:357","NEWT:74381","NEWT:2010198","NEWT:457428","NEWT:84023","NEWT:589904","NEWT:559292","NEWT:2762","NEWT:1174673","NEWT:28035","NEWT:1313166","NCBITaxon:138","NEWT:28038","NEWT:1422","NEWT:1423","NEWT:4932","NEWT:1205903","NEWT:3603","NEWT:2756","NEWT:2759","NEWT:38293","NEWT:1428","NEWT:339","NEWT:2751","NEWT:139077","NEWT:391904","NEWT:12922","NEWT:316277","NEWT:163159","NEWT:1306413","NEWT:2813599","NEWT:518766","NEWT:2664373","NEWT:190802","NEWT:1213463","NEWT:9417","NEWT:1216979","NEWT:7237","NEWT:5059","NEWT:31631","NEWT:509924","NEWT:28450","NEWT:115104","NEWT:5061","NEWT:8570","NEWT:127569","NEWT:5062","NEWT:1408434","NEWT:1241935","NEWT:220668","NEWT:9407","NEWT:7227","NEWT:9402","NEWT:415540","NEWT:336722","NEWT:675060","NEWT:416870","NEWT:334542","NEWT:313625","NEWT:51240","NEWT:27592","NEWT:5052","NEWT:227289","NEWT:7215","NEWT:196139","NEWT:5037","NEWT:651182","NEWT:2172103","NCBITaxon:59201","NEWT:1876254","NEWT:434271","NEWT:1915","NEWT:747679","NEWT:66712","NEWT:42752","NEWT:746360","NEWT:89764","NEWT:543734","NEWT:49390","NEWT:9870","NEWT:256648","NEWT:435590","NEWT:1902","NEWT:208526","NEWT:1908","NEWT:1169740","NEWT:217459","NEWT:216129","NEWT:1245012","NEWT:171250","NEWT:528135","NEWT:1637254","NCBITaxon:38727","NEWT:74790","NEWT:2829","NEWT:126279","NEWT:1428006","NEWT:62335","NEWT:366581","NEWT:757417","NEWT:216599","NEWT:216595","NEWT:40559","NEWT:51208","NEWT:216597","NEWT:1607839","NEWT:1283300","NEWT:1218707","NEWT:1204551","NEWT:99586","NEWT:228577","NEWT:2060328","NEWT:48038","NEWT:392021","NEWT:436486","NEWT:51655","NEWT:1933298","NEWT:260707","NEWT:121845","NEWT:8595","NEWT:7263","NEWT:186763","NEWT:447135","NEWT:185431","NEWT:216591","NEWT:29760","NEWT:260704","NEWT:703612","NEWT:260705","NEWT:546805","NEWT:1505907","NEWT:1216981","NEWT:1249885","NEWT:1415167","NEWT:36036","NEWT:9430","NEWT:71251","NEWT:208963","NEWT:208964","NEWT:181088","NEWT:48498","NEWT:669202","NEWT:6306","NEWT:4120","NEWT:37334","NCBITaxon:10357","NCBITaxon:10358","NCBITaxon:10359","NEWT:1203258","NEWT:49317","NEWT:696811","NEWT:9808","NEWT:839","NEWT:92519","NEWT:943925","NEWT:4111","NEWT:37349","NEWT:4113","NEWT:837","NEWT:640459","NEWT:72568","NEWT:36015","NEWT:51641","NEWT:35168","NEWT:81866","NEWT:1262469","NEWT:277944","NEWT:48479","NEWT:61435","NEWT:627025","NEWT:1097677","NEWT:375939","NEWT:712938","NEWT:3258","NEWT:112018","NEWT:4100","NEWT:1159556","NEWT:1076","NEWT:4102","NEWT:1028688","NEWT:4101","NEWT:6763","NEWT:803","NEWT:29722","NEWT:2847306","NEWT:468911","NEWT:13373","NEWT:7604","NEWT:6756","NEWT:818","NEWT:135588","NEWT:1061","NEWT:29719","NEWT:6754","NEWT:452589","NEWT:509068","NEWT:4577","NEWT:813","NEWT:1365218","NEWT:337677","NEWT:1042986","NEWT:1911079","NEWT:69373","NEWT:427577","NEWT:264203","NEWT:1480154","NEWT:9858","NEWT:9859","NEWT:512623","NEWT:1274414","NEWT:2907833","NEWT:38626","NEWT:10298","NEWT:10299","NEWT:1087439","NEWT:660025","NEWT:61408","NEWT:1355477","NEWT:90371","NEWT:55194","NEWT:2907829","NEWT:4153","NEWT:11137","NEWT:278839","NEWT:35128","NEWT:39966","NEWT:7668","NEWT:2929635","NEWT:58228","NEWT:915099","NEWT:1143193","NEWT:1122233","NEWT:859313","NEWT:59540","NEWT:9838","NEWT:9839","NEWT:546414","NEWT:11167","NEWT:9835","NEWT:7650","NEWT:4146","NEWT:5478","NEWT:6326","NEWT:7656","NEWT:5476","NEWT:5477","NEWT:1882945","NEWT:746707","NEWT:299767","NEWT:28835","NEWT:1206781","NEWT:5480","NEWT:1274432","NEWT:222863","NEWT:2053667","NEWT:859321","NEWT:1274426","NEWT:9825","NEWT:1274423","NEWT:393765","NEWT:104421","NEWT:39946","NEWT:9823","NEWT:95602","NEWT:39947","NEWT:1274420","NEWT:859314","NEWT:578458","NEWT:60520"],"pubmed":["41963076"],"ORCID":["0000-0002-3977-4172"]}}