Prideapplication/xmlftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRMquantificationresult.xlsxftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/checksum.txtftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_WT176.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BM78.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_WT180.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BD51.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BM79.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AD97.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_BEDP_102725HYP2-0927_6k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CDEL35.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_WT177.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AD99.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AD98.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CDEL36.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BD50.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AD100.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_WT178.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CDEL37.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AM46.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_WT2_091926HYP2-0812_Q6500-8500_12k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BM80.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_WT179.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AM47.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CMUT35.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CDEL38.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BD53.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BD52.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AD96.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_HAPD_112525HAPD-1112_Q6500-8500_12k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_PDEA_112525PDEA-1112_Q6500-8500_12k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CDEL39.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_WT2_091926HYP2-0812_6k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CMUT36.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CMUT40.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_AHBE_102725HYP2-1016_6k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_WT1_091826HYP2-0804_Q6500-8500_12k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AM36.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PDE6Nterminal_DDA_inclu.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CMUT37.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_WT1_091826HYP2-0804_6k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BM76.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AM37.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CMUT38.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_AM33.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_HAPD_112525HAPD-1112_6k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_BEDP_102725HYP2-0927_Q6500-8500_12k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_CMUT39.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_AHBE_102725HYP2-1016_Q6500-8500_12k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BM77.rawftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/nativeMS_PDEA_112525PDEA-1112_6k.RAWftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073183/PRM_BD49.rawprimaryOK200kpalczew@uci.eduFangyuan GaoSRM/MRMData-dependent acquisitionMass SpectrometryTop-down proteomicsBottom-up proteomicsPhosphodiesterase 6Outer segmentRod photoreceptorCgmpVisual transductionhttps://www.ebi.ac.uk/pride/archive/projects/PXD073183Photoreceptor CellRetinaTargeted MS-based protein quantification Mouse eyecups were harvested and resuspended in a buffer containing 8 M urea, 0.1 M Tris-HCl (pH 8.5), and a protease inhibitor cocktail (cOmplete™ Protease Inhibitor Cocktail, Roche, Basel, Switzerland). The suspension was sonicated on ice for 4 min, followed by centrifugation at 12,000 x g for 10 min at 4 oC. The supernatant was collected, enriched with SIL peptides, and digested with trypsin (Sequencing-grade modified trypsin, Promega), according to the filter-aided sample-preparation (FASP) protocol (77). The tryptic digests were recovered in the flow-through after spin-filtration, and an additional wash with water. The combined peptide samples were vacuum-dried and dissolved in 20 μl of 0.1% formic acid (FA) in water. For parallel reaction monitoring (PRM), peptides were separated over a 25-min gradient on an Acquity UPLC® BEH C18 column (1.7 μm, 2.1 mm × 100 mm, Waters Corporation). MS2 scan parameters were set to select the m/z ratio of the endogenous and SIL versions of the targeted peptides with defined elution time windows. MS1 scans were acquired at an m/z range of 300–1000, mass resolution of 70,000, automatic gain control (AGC) target of 1e6, and maximum ion injection time of 50 ms. The PRM scans were acquired at a resolution of 17,500, an AGC target value of 1e5, a maximum ion injection time of 50 ms, an isolation window of 2.0 m/z, and the HCD energy was 25%. Native MS PDE6 was extracted with a hypotonic solution from retinas obtained from 24 h dark-adapted mice. The ROS pellet was then resuspended in 50 of hypotonic buffer containing 2 mM HEPES, pH 7.4, 1 mM DTT, 0.1 mM EDTA and centrifuged immediately for 15 min at 30,000 x g at 4 C with the supernatant stored as the “first hypotonic wash.” PDE6 was then extracted from the pellet by a second hypotonic wash with 75 l of hypotonic buffer, incubated on ice for 2 h with occasional mixing, before centrifugation for 30 min (30,000 x g at 4 oC). The protein complexes in the PDE6 extracts were analyzed by native MS as previously described by Liu et al.(80). In short, native MS analyses were performed using a Q Exactive UHMR mass spectrometer (Thermo Fisher Scientific). Spray voltages were optimized for each ionization source: 4.0–4.3 kV for the HESI source and 2.0–2.8 kV for the EASY-Spray source. The capillary temperature was maintained between 275 and 300 °C. Ion transfer target m/z and detector optimization modes were set to "high m/z" and "low m/z", respectively. In-source trapping voltage was optimized between −50 and −250 V depending on the analyte characteristics. Data were acquired at resolution settings ranging from 6000 to 12,000 (at m/z 400) with transient averaging enabled. MS analysis of the N-terminal part of PDE6 subunits The PDE6 fraction was diluted with 50 mM NH4HCO3 and then free trypsin was added to the protein solution at a ratio of 1:50 and incubated at 37 °C overnight. The obtained solution was acidified with 0.5% acetic acid. Then, the peptide mixture was subjected to C18 solid-phase extraction (The Nest Group, Inc., Southborough, MA, USA) for desalting and subsequently vacuum-dried. Proteomics data were acquired via LC-MS/MS using an UltiMate 3000 UHPLC (Thermo Fisher Scientific) coupled in-line with an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) using an ESI nanospray source. Mobile phase B comprised 0.1% FA in acetonitrile. The total flow rate was 300 nL nL min-1, and peptides were separated over a 57 min gradient from 4% to 25% buffer B (total run time 90 min per sample) on an Acclaim PepMap RSLC column (50 cm x 75 μm, Thermo Fisher Scientific). Survey (MS) scans were acquired in the Orbitrap (FT, Thermo Fisher Scientific) with an automated gain control (AGC) target of 8E5, a maximum injection time of 50 ms, and a dynamic exclusion of 30 s across the scan range of 375–1800 m/z. MS/MS spectra were acquired in data-dependent acquisition mode at top speed for 3 s per cycle, with an inclusion list of peptides corresponding to the N-terminal part of PDE6A (MGEVTAEEVEK, FLDSNIGFAK, QYYNFHYR, VISDLLGAK) and the N-terminal part of PDE6B (LSEEQVR, LDGNPTFAHQYFGK, and AGACEDGWLADCGSLR).The AGC target was set to 1E4 with a maximum injection time of 35 ms. Ions were subjected to stepped-energy higher-energy collision dissociation (seHCD) fragmentation at a normalized collision energy (NCE) of 20 ± 5%.PrideNot availableTargeted MS-based protein quantification Proteotypic peptides for targeted analysis were selected from DDA LC-MS/MS data files using MaxQuant (version 1.5.2.8) and the Uniprot mouse database (updated on May 21st, 2018). The criteria for peptide identification were mass tolerances of 20 ppm for precursor ions and 0.5 Da for MS/MS fragmentation, two missed cleavages, cysteine carbamidomethylation as the static modification, oxidation of methionine as the variable modification, and a 1% false discovery rate (FDR). The Skyline pipeline was used for processing the data for PRM quantification (78). Peak picking and peak boundaries were carried out by the software algorithm and manually adjusted based on the overlapping precursor and product peaks. native MS The m/z mass spectra were processed with a Bayesian deconvolution algorithm using UniDec software (81) to obtain the distribution of true protein masses. MS analysis of the N-terminal part of PDE6 subunits The raw LC-MS/MS data files were analyzed using MaxQuant (Max Planck Institute of Biochemistry, Martinsried, Germany, version 2.5.2.0), with the spectra searched against the UniProt mouse database (updated on Oct. 20th, 2025). For identification of the peptides, the mass tolerances were 20 ppm for initial precursor ions and 0.5 Da for fragment ions. Two missed cleavages in tryptic digests were allowed. Cys residues were set as a static modification, while oxidation of Met residue was set as a variable modification. Filtering for the peptide identification was set at a 1% false discovery rate (FDR)ProteomicsKrzysztof PalczewskiPARTIAL1Brunason Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, Gavin Herbert Eye Institute, University of California, Irvine, Irvine, CA, 92697, USA; 2Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA, 92697, USA; 3Department of Chemistry, University of California, Irvine, Irvine, CA, 92697, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697, USAMus Musculus (mouse)10.1073/PNAS.2604088123fangyuag@hs.uci.eduUniversity of California, IrvineUnited StatesfalseFunctional Asymmetry and Essential Structural Roles of PDE6α and PDE6β Subunits in Rod Photoreceptor IntegrityTetrameric rod phosphodiesterase 6 (PDE6) critically regulates cyclic GMP (cGMP) levels in rod photoreceptors comprised of catalytic core α/β heterodimer and two small inhibitory γ subunits. While the rod PDE6 catalytic core is a heterodimer, the cone isoform is an α'/α' homodimer. This structural difference suggests unique roles for each rod subunit. Due to the lack of heterologous expression systems, we leveraged novel transgenic mouse models to examine the functional contributions of the N-terminal Pt motifs and the individual catalytic subunits in vivo. We generated four independent mouse lines: N-terminal deletions (PDE6αΔ2-48AA and PDE6βΔ2-46AA) and active-site mutations (PDE6αH599A and PDE6βH597A) designed to selectively abolish catalytic activity. Native mass spectrometric analysis of heterozygous mice confirmed that only wild-type enzyme was produced. While homozygous mutations in all lines caused complete photoreceptor degeneration, analysis of heterozygous lines revealed a disproportionately greater impact of PDE6β mutations on protein stability, enzymatic activity, and visual function compared to PDE6α. These findings establish that both active subunits are essential for rod integrity but uncover a significant functional asymmetry within the PDE6αβ heterodimer. Our results demonstrate that the PDE6β subunit plays a more dominant role in maintaining the structural and functional pool of the PDE6 enzyme.2026-05-252026-01-17PXD073183NEWT:3555NEWT:71647NEWT:35554NEWT:32046NEWT:544496NEWT:2042546NEWT:32049NEWT:1392696NEWT:259447NEWT:45351NEWT:445974NEWT:43179NEWT:180454NEWT:5722NCBITaxon:1280NEWT:1129NEWT:309807NEWT:55153NEWT:309800NEWT:281395NEWT:1211601NEWT:876138NEWT:44271NEWT:10036NEWT:1590NEWT:498019NEWT:1351NEWT:1438992NEWT:1352NEWT:2649997NEWT:1147161NEWT:263737NEWT:224326NEWT:1333499NCBITaxon:79857NEWT:1096976NEWT:95648NEWT:1589NEWT:135622NEWT:1349NEWT:96731NEWT:383379NEWT:10029NEWT:913645NEWT:44491NEWT:556484NEWT:317447NEWT:4688NEWT:7955NEWT:7959NEWT:2261NEWT:31156NEWT:3112NEWT:4442NEWT:192875NEWT:1214915NEWT:12637NEWT:59729NEWT:2164133NEWT:108061NEWT:1316931NEWT:224308NEWT:3347NEWT:511145NEWT:931281NEWT:658457NEWT:1310161NEWT:77133NEWT:295358NEWT:145481NCBITaxon:79824NCBITaxon:4563NEWT:5755NEWT:44689NEWT:44447NEWT:5759NEWT:1736231NEWT:5518NEWT:1392NEWT:498217NEWT:2242NEWT:11320NEWT:286NEWT:391619NEWT:246196NEWT:287NEWT:246197NEWT:633149NEWT:10239NEWT:44685NEWT:161934NEWT:1148NEWT:5508NEWT:5507NEWT:410661NEWT:55571NEWT:35500NEWT:1140NCBITaxon:2157NEWT:1143NEWT:4896NEWT:1390NEWT:11557NEWT:1094343NEWT:1336795NEWT:644042NEWT:294NEWT:1773NEWT:1182590NEWT:3712NEWT:82380NEWT:935293NEWT:375146NEWT:118698NEWT:263NEWT:118696NEWT:52283NEWT:284812NEWT:8175NEWT:43330NEWT:1603293NEWT:408169NEWT:44664NEWT:47946NEWT:3702NEWT:243277NEWT:7067NEWT:118694NEWT:2850NEWT:118691NEWT:33548NEWT:274NEWT:408172NEWT:408170NEWT:96794NEWT:3708NEWT:332648NEWT:44670NEWT:536231NEWT:376219NEWT:1436733NEWT:460519NEWT:1247411NEWT:572307NEWT:1432138NEWT:1424507NEWT:1194599NEWT:272844NEWT:9483NEWT:333760NEWT:1513458NCBITaxon:40559NEWT:506599NEWT:84588NEWT:1679718NEWT:480NEWT:67767NEWT:46835NEWT:109757NEWT:582580NEWT:300852NEWT:1502NEWT:376686NEWT:95486NEWT:508771NEWT:1883446NEWT:253NCBITaxon:2759NEWT:1233435NEWT:93061NEWT:93062NCBITaxon:4932NEWT:644223NEWT:235443NEWT:108458NEWT:272623NEWT:272624NEWT:320637NEWT:983964NEWT:118499NEWT:32644NEWT:527796NEWT:499175NEWT:109779NEWT:3745NEWT:1715989NCBITaxon:4751NEWT:476272NEWT:3747NEWT:195051NEWT:3988NEWT:1255228NEWT:649908NEWT:410289NEWT:373153NEWT:352472NEWT:1071661NEWT:360094NEWT:470NEWT:41364NEWT:1313NEWT:39491NCBITaxon:5811NEWT:29491NEWT:2014887NEWT:33952NEWT:261756NEWT:571256NEWT:40483NEWT:272634NEWT:9031NEWT:1872122NEWT:7091NEWT:141262NEWT:108931NEWT:1055524NEWT:4087NEWT:9778NEWT:306NEWT:150475NEWT:303NEWT:267872NEWT:7111NEWT:347515NEWT:9534NEWT:5180NEWT:256737NEWT:4090NEWT:4092NEWT:9541NEWT:8694NEWT:4093NEWT:4096NEWT:8692NEWT:185579NEWT:941442NEWT:13076NEWT:1226408NEWT:7108NEWT:40479NEWT:4076NEWT:317NEWT:1006581NEWT:885318NEWT:39488NEWT:550NEWT:4081NEWT:273068NEWT:554NEWT:98334NEWT:451516NEWT:4084NEWT:36185NEWT:1225786NEWT:7574NEWT:1715256NEWT:30640NEWT:575412NEWT:929793NEWT:29204NEWT:28112NEWT:114155NEWT:6494NEWT:6491NEWT:507601NEWT:186441NEWT:643680NEWT:214092NCBITaxon:6157NEWT:6239NEWT:162425NEWT:216257NEWT:102169NEWT:9986NEWT:4054NEWT:8654NEWT:8658NEWT:1268063NEWT:8655NEWT:1263854NEWT:118503NEWT:2059687NEWT:160488NEWT:28104NEWT:407821NCBITaxon:2NEWT:985076NEWT:1215323NEWT:986NEWT:52641NEWT:7159NEWT:28532NEWT:353152NEWT:8496NEWT:27448NEWT:40674NEWT:1194669NEWT:243230NEWT:1080772NEWT:519NEWT:8479NEWT:8485NEWT:6063NEWT:49240NEWT:104395NEWT:6289NEWT:6287NEWT:300641NEWT:727NEWT:9796NEWT:725NEWT:469008NEWT:256318NEWT:9321NEWT:206411NCBITaxon:6191NEWT:596153NEWT:229533NEWT:1836NEWT:80863NEWT:105231NEWT:52638NEWT:57075NEWT:4097NEWT:8697NEWT:8695NEWT:884204NEWT:9785NEWT:6279NEWT:1123869NEWT:9544NEWT:7370NEWT:83906NCBITaxon:780NCBITaxon:1502NEWT:6282NEWT:1134506NEWT:38783NEWT:4006NEWT:6426NCBITaxon:5476NEWT:6669NEWT:9935NEWT:89453NEWT:287889NEWT:51515NEWT:588596NEWT:51511NEWT:8845NEWT:92867NEWT:92866NEWT:5334NEWT:51750NEWT:202950NEWT:382352NEWT:413071NCBITaxon:714NEWT:632957NEWT:9925NEWT:89462NCBITaxon:9606NEWT:8839NEWT:90690NEWT:4232NEWT:2758385NEWT:11298NEWT:171101NEWT:714NEWT:421932NEWT:312017NEWT:196627NEWT:200308NEWT:200302NEWT:870435NEWT:284593NEWT:100392NEWT:5315NEWT:9913NEWT:265872NEWT:105841NEWT:337330NEWT:2666255NEWT:2285NEWT:999810NCBITaxon:5693NEWT:1392998NEWT:380394NEWT:981087NEWT:180066NEWT:226900NEWT:231490NEWT:430615NEWT:326423NEWT:452467NEWT:198822NEWT:36111NEWT:326424NEWT:1416333NEWT:1678078NEWT:749906NEWT:418985NEWT:1817808NEWT:749907NEWT:145263NEWT:694569NEWT:27606NEWT:9739NEWT:59202NEWT:9975NEWT:8643NEWT:51953NEWT:3197NEWT:860688NEWT:13443NEWT:8644NEWT:36329NEWT:62615NCBITaxon:3044782NEWT:160235NEWT:72407NEWT:8639NEWT:349741NEWT:409859NEWT:157295NEWT:2096NEWT:192222NEWT:9721NEWT:137221NEWT:9963NEWT:1617910NEWT:565050NEWT:409860NEWT:2785785NEWT:411901NEWT:8626NEWT:105884NCBITaxon:9615NEWT:8621NEWT:58334NEWT:1193501NEWT:58331NEWT:6689NEWT:8624NEWT:5116NEWT:8623NEWT:298315NEWT:8618NEWT:70448NEWT:698936NEWT:9940NEWT:3168NEWT:521001NEWT:192011NEWT:1813887NEWT:118702NEWT:118700NEWT:224911NEWT:118705NCBITaxon:11552NEWT:6945NEWT:184922NEWT:6703NEWT:2104NEWT:2NEWT:157546NEWT:474186NEWT:38942NEWT:1493NEWT:307972NEWT:1496NEWT:2102NEWT:660469NEWT:45474NEWT:1590841NEWT:4513NEWT:1247NEWT:70667NEWT:6938NCBITaxon:10407NEWT:1736309NEWT:4751NEWT:10360NEWT:47664NEWT:317548NEWT:15819NEWT:3654NEWT:237561NEWT:5833NEWT:6928NEWT:1239NEWT:36745NEWT:272563NEWT:82688NEWT:3885NEWT:3888NEWT:5821NEWT:44139NCBITaxon:4896NEWT:6915NEWT:3649NEWT:101510NEWT:28903NEWT:3880NEWT:272559NEWT:28909NEWT:515849NEWT:3641NEWT:466585NEWT:1000589NEWT:85963NEWT:85962NEWT:143361NEWT:12993NEWT:398580NEWT:4565NEWT:1264690NEWT:515619NEWT:1299NEWT:34305NCBITaxon:183674NEWT:167387NEWT:84645NEWT:44586NEWT:626528NEWT:139927NEWT:4558NEWT:209285NEWT:5888NEWT:211586NEWT:747078NEWT:1282NEWT:1283NEWT:431241NEWT:4550NEWT:1000561NEWT:2133NEWT:4555NEWT:294381NEWT:197NEWT:1390363NEWT:35889NEWT:288705NEWT:29176NEWT:4787NEWT:3217NEWT:4547NEWT:3218NEWT:556182NEWT:1270NEWT:374990NEWT:1432059NEWT:156471NEWT:4784NEWT:360106NEWT:156476NEWT:4543NEWT:4785NEWT:360104NEWT:10117NEWT:10116NEWT:1280NEWT:10358NEWT:1735272NEWT:83334NEWT:83332NEWT:317513NEWT:580240NEWT:294128NEWT:11676NEWT:100226NEWT:4530NEWT:75058NEWT:13616NEWT:296543NEWT:173NEWT:316435NEWT:42157NEWT:1895NEWT:1034304NEWT:105023NEWT:866628NEWT:64152NEWT:4924NEWT:749200NEWT:9378NEWT:990346NEWT:145953NEWT:257309NEWT:100816NEWT:230741NCBITaxon:1313NEWT:44544NEWT:4911NEWT:645463NEWT:129249NEWT:1077286NEWT:943129NEWT:990119NEWT:77216NEWT:1111708NEWT:227321NEWT:493760NEWT:106590NEWT:260710NEWT:257313NEWT:3827NEWT:400772NEWT:128161NEWT:930945NEWT:106592NEWT:46704NEWT:1187947NEWT:269796NEWT:10312NCBITaxon:1773NEWT:54571NEWT:9598NEWT:8030NEWT:1423772NEWT:1639NEWT:188229NEWT:3818NEWT:4909NEWT:759272NEWT:432359NEWT:1182263NEWT:943146NEWT:2711NEWT:537457NEWT:9103NEWT:715877NEWT:862238NEWT:29159NEWT:10306NEWT:8022NEWT:145943NEWT:595536NEWT:240906NEWT:593117NEWT:89920NEWT:29158NEWT:3633NEWT:3635NEWT:5811NEWT:180923NEWT:1328881NEWT:411483NEWT:76336NEWT:884019NEWT:198215NEWT:411490NEWT:200298NEWT:169963NEWT:225117NEWT:746128NEWT:43665NEWT:1266464NEWT:367830NEWT:178616NEWT:375451NEWT:357NEWT:9172NEWT:106178NEWT:411469NEWT:84023NEWT:1679NEWT:559292NEWT:411464NEWT:411460NEWT:2762NEWT:1174673NEWT:1328426NEWT:562NEWT:411470NEWT:2094720NCBITaxon:2697049NCBITaxon:138NEWT:28038NEWT:1663NEWT:62141NEWT:1423NEWT:4932NEWT:3603NEWT:2759NEWT:3847NEWT:38293NEWT:1428NEWT:327159NEWT:178876NEWT:1660NEWT:327160NEWT:573NEWT:571NEWT:241368NEWT:42528NEWT:2664373NEWT:190802NEWT:9417NEWT:1216979NEWT:7237NEWT:115104NEWT:1121114NEWT:663NEWT:1081927NEWT:1238993NEWT:67825NEWT:187878NEWT:220668NEWT:1821314NEWT:1249668NEWT:426430NEWT:9407NEWT:9646NEWT:5286NEWT:7227NEWT:7469NEWT:9402NEWT:9644NEWT:415540NEWT:675060NEWT:334542NEWT:313625NEWT:27592NEWT:426428NEWT:63459NEWT:1276815NEWT:588858NEWT:9638NEWT:9639NCBITaxon:11071NEWT:242231NEWT:197221NEWT:7215NEWT:2172103NCBITaxon:59201NEWT:7460NEWT:504457NEWT:434271NEWT:747679NEWT:66712NEWT:42752NEWT:746360NEWT:89764NEWT:470150NEWT:543734NEWT:73239NEWT:38018NEWT:226186NEWT:9870NEWT:8782NEWT:115357NEWT:435590NEWT:1902NEWT:1908NEWT:13164NEWT:216129NEWT:2664179NEWT:623NEWT:7038NEWT:6192NCBITaxon:38727NEWT:2829NEWT:366581NEWT:216599NEWT:216595NEWT:40559NEWT:9689NEWT:8355NEWT:633NEWT:9685NEWT:7029NEWT:1283300NEWT:9694NEWT:186990NEWT:632NEWT:6183NEWT:99586NEWT:630NEWT:334747NEWT:61235NEWT:343691NEWT:15368NEWT:1491466NEWT:436486NEWT:51655NEWT:170187NEWT:260707NEWT:8595NEWT:186763NEWT:185431NEWT:29760NEWT:260704NEWT:1719NEWT:703612NEWT:260705NEWT:692672NEWT:2697049NEWT:1216981NEWT:1109443NEWT:7004NEWT:9669NCBITaxon:6073NEWT:1089448NEWT:1415167NEWT:1329349NEWT:607699NEWT:208963NEWT:208964NEWT:27337NEWT:575584NEWT:3039NEWT:8727NEWT:8726NEWT:10090NEWT:4120NEWT:5693NEWT:2186NEWT:8724NEWT:8723NEWT:283166NEWT:37334NCBITaxon:10359NEWT:1203258NEWT:242619NEWT:49317NEWT:160791NEWT:34073NEWT:696811NEWT:373995NEWT:5689NEWT:544404NEWT:37349NEWT:4113NEWT:837NEWT:36015NEWT:81866NEWT:5691NEWT:48479NEWT:61435NEWT:627025NEWT:1097677NEWT:375939NEWT:61674NEWT:1117957NEWT:6526NEWT:8709NEWT:4100NEWT:1159556NEWT:5671NEWT:1076NEWT:4102NEWT:4101NEWT:6763NEWT:8700NEWT:803NEWT:29722NEWT:1692259NEWT:1486917NEWT:468911NEWT:817NEWT:7604NEWT:818NEWT:135588NEWT:1843183NEWT:5661NEWT:51866NEWT:29719NEWT:58002NEWT:5665NEWT:6997NEWT:452589NEWT:4577NEWT:813NEWT:5664NEWT:2157NEWT:146479NEWT:1042986NEWT:1911079NEWT:69373NEWT:206189NEWT:264203NEWT:1480154NEWT:1274414NEWT:9612NEWT:38626NEWT:38865NEWT:3077NEWT:9615NEWT:10299NEWT:6100NEWT:112273NEWT:1147787NEWT:1440772NEWT:1355477NEWT:9605NEWT:9606NEWT:90371NEWT:641501NEWT:4153NEWT:278839NEWT:7668NEWT:915099NEWT:74940NEWT:13551NEWT:10041NEWT:9600NEWT:1450511NEWT:1143193NEWT:174934NEWT:179392NEWT:2823525NEWT:9838NEWT:9839NEWT:546414NEWT:3055NEWT:11167NEWT:4146NEWT:5478NEWT:6326NEWT:5476NEWT:1450520NEWT:1042311NEWT:299767NEWT:28835NEWT:1274432NEWT:2053667NEWT:1274426NEWT:9825NEWT:1274423NEWT:393765NEWT:39946NEWT:9823NEWT:95602NEWT:8732NEWT:314539NEWT:39947NEWT:1274420NEWT:578458