{"database":"Pride","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Tabular":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/pg_matrix_exp_MSPC_6aug25_v3.tsv"],"Wiff":["ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/HL_run3.wiff","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/HL_run1.wiff.scan","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/Healthy_skin_run2.wiff","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/MSPC_KA_run2.wiff","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/HL_run1.wiff","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/MSPC_KA_run1.wiff.scan","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/MSPC_KA_run2.wiff.scan","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/MSPC_KA_run3.wiff.scan","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/HL_run3.wiff.scan","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/Healthy_skin_run1.wiff.scan","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/MSPC_KA_run1.wiff","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/Healthy_skin_run3.wiff","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/HL_run2.wiff.scan","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/Healthy_skin_run3.wiff.scan","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/Healthy_skin_run2.wiff.scan","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/MSPC_KA_run3.wiff","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/Healthy_skin_run1.wiff","ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073302/HL_run2.wiff"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"labhead_mail":["Catalin.Marinescu@independent-research.ro"],"submitter":["Roua Popescu"],"technology_type":["Mass Spectrometry","Bottom-up proteomics","Data-independent acquisition","SWATH MS"],"software":[""],"submitter_keywords":["Human","Lc-ms","Skin","Mspc"],"full_dataset_link":["https://www.ebi.ac.uk/pride/archive/projects/PXD073302"],"tissue":["Keratinocyte","Skin"],"sample_protocol":["Biopsies were collected in cold PBS, then 50 mg from each sample was disrupted in a Mini-Beadbeater (Biospec). Samples were then centrifuged for 20 minutes at 18,000 g and the protein concentration from the supernatant was quantified by Bradford assay. A total of 50 µg of protein containing supernatant was diluted with urea buffer to the final volume of 40 µL, followed by the addition of 25 µL of 100 mM DTT and incubation at room temperature for 60 minutes. The sample was then alkylated with 26.25 µL of 300 mM iodoacetamide and kept in the dark at room temperature for an additional 90 minutes followed by a quenching reaction with 50 µL of DTT 100mM at room temperature for 60 minutes. The reaction mix was finally diluted to 500 µL with 50 mM ammonium bicarbonate, followed by addition of trypsin (1 µg /µL; Trypsin Gold, V528A, Promega) at a 1:50 enzyme-to-substrate ratio, with incubation at 37°C for 16 hours. The reaction was stopped by adding 3 µL of formic acid. Peptides were cleaned up using SPE (MonoSpin C18, GL Sciences), dried at speed-vac and rehydrated to 2 µg/µL using 5% ACN + 0.1% HCOOH. The peptides concentration was measured using NanoDrop 1000 (Thermofisher, USA). From each sample, 5 µg peptides were loaded into Nano Acquity HPLC and separated using an Eksigent 5C18-CL-120 (300 µM ID, 150 mm length) column coupled with an AB SCIEX TRIPLE TOF 5600+ mass spectrometer. Peptide samples (5 µg) were injected using Solvent A (0.1% formic acid) and separated on a gradient ranging from 5% to 90% Solvent B (0.1% formic acid in acetonitrile) over 90 minutes, at a flow rate of 5 µL /min, with the column maintained at 55 °C. Each sample was analysed in triplicate. Mass spectrometric analysis was carried out using electrospray ionization in positive mode, with a spray voltage of 5500 V and a source temperature of 200°C. Data acquisition was performed on a TRIPLE TOF 5600+ system in DIA SWATH-MS mode with 64 variable isolation windows. The MS1 survey scans covered an m/z range of 400–1250, while MS2 spectra were collected in high-sensitivity mode from 100 to 2000 m/z. The accumulation period was set to 0.049 s, and ions were scanned within 55 ms windows in high-sensitivity mode, giving a total cycle time of 3.5 s."],"repository":["Pride"],"quantification_method":["Not available"],"modification":[""],"data_protocol":["Peptides and protein identification was performed using PeakView 2.2, Skyline 22.2.0.255, and DIA-NN 1.9. The raw spectra were searched against the fasta file containing the complete human reference proteome (UniProt, UP000005640, June 2025, 83 587 entries), with a precursor m/z range of 400 to 1250, and trypsin was chosen as the digestion enzyme. The data were searched with MBR enabled and robust LC was employed as the quantification strategy, with a false discovery rate (FDR) set at 0.01. C carbamidomethylation and Ox(M) modifications were considered during the search. Retention time-dependent normalization was applied, and quantitation was performed using the MaxLFQ algorithm in DIA-NN."],"omics_type":["Proteomics"],"labhead":["George Catalin Marinescu"],"instrument_platform":[""],"labhead_affiliation":["Asociatia Independent Research, Bucharest, Romania"],"submission_type":["PARTIAL"],"species":["Homo Sapiens (human)"],"submitter_mail":["roua@independent-research.ro"],"publication":["42123364 Dobre A, Fertig TE, Niculae AM, Cohn AM, Curici A, Andrei RT, Marta DS, Peteu VE, Popescu RG, Marinescu GC, Turcu G, Forsea AM, Ion DA, Gherghiceanu M, Nedelcu RI. Mast Cells Are a Reservoir of NLRP1 in Human Skin. Int J Mol Sci. 2026 27(9):3775 10.3390/ijms27093775"],"submitter_affiliation":["Independent Research Association"],"submitter_country":["Romania"],"pubmed_abstract":["NLRP1 is an inflammasome sensor protein expressed in barrier tissues of humans. Its activation in response to microbes or cellular stress triggers a cascade of molecular events, leading up to IL1β-driven inflammation and pyroptosis. Rare germline mutations of NLRP1 cause its persistent activation, resulting in autoinflammatory syndromes. Multiple self-healing palmoplantar carcinoma (MSPC) is one such syndrome, characterized by the appearance of recurrent keratoacanthomas (KAs) on the palms and soles. Here, we aimed to compare the subcellular localization of mutant NLRP1 in lesions from an MSPC patient to wild-type NLRP1 in non-MSPC-KAs and in skin from healthy donors. Using mass spectrometry, immunohistochemistry and immunoelectron tomography, we found that NLRP1 localized to mast cell granules in all MSPC lesions but also in healthy skin, a novel finding which implicates these cells in NLRP1-associated responses in human skin. Moreover, we found that mast cells expressing the A66V pathogenic variant of NLRP1 overpopulated MSPC-KAs, infiltrated the epidermis and degranulated, a behavior not seen in other lesions from this study. The released granules had the highest NLRP1 protein content and also contained NLRP3 and IL1β, suggesting the coexistence of inflammasome pathways within mast cells. Taken together, our findings propose cutaneous mast cells as a previously unrecognized NLRP1 reservoir in health and disease."],"pubmed_title":["Mast Cells Are a Reservoir of NLRP1 in Human Skin."],"pubmed_authors":["Dobre Alexandra A, Fertig Tudor Emanuel TE, Niculae Andrei Marian AM, Cohn Adelina Maria AM, Curici Antoanela A, Andrei Razvan Theodor RT, Marta Daciana Silvia DS, Peteu Victor Eduard VE, Popescu Roua Gabriela RG, Marinescu George Catalin GC, Turcu Gabriela G, Forsea Ana Maria AM, Ion Daniela Adriana DA, Gherghiceanu Mihaela M, Nedelcu Roxana Ioana RI"],"additional_accession":[]},"is_claimable":false,"name":"Multiple self-healing palmoplantar carcinoma (MSPC) proteomics","description":"Multiple self-healing palmoplantar carcinoma (MSPC) is one such syndrome, characterized by the appearance of recurrent keratoacanthomas (KAs) on the palms and soles. Here, we compare healthy skin, hyperkeratotic inflammatory lesion (HL), and MSPC-associated KA samples.","dates":{"publication":"2026-06-08","submission":"2026-01-21"},"accession":"PXD073302","cross_references":{"TAXONOMY":["NEWT:6945","NEWT:184922","NEWT:6703","NEWT:3555","NEWT:2","NEWT:157546","NEWT:35554","NEWT:38942","NEWT:307972","NEWT:32046","NEWT:544496","NEWT:2102","NEWT:2042546","NEWT:45351","NEWT:43179","NEWT:4513","NEWT:5722","NEWT:1247","NEWT:376741","NEWT:55153","NCBITaxon:10407","NEWT:1736309","NEWT:309800","NEWT:281395","NEWT:10360","NEWT:1211601","NEWT:876138","NEWT:47664","NEWT:3654","NEWT:237561","NEWT:5833","NEWT:6928","NEWT:10036","NEWT:36745","NEWT:498019","NEWT:1351","NEWT:1438992","NEWT:1352","NEWT:2649997","NEWT:272563","NEWT:224326","NCBITaxon:79857","NEWT:1096976","NEWT:82688","NEWT:95648","NEWT:3885","NEWT:3888","NEWT:5821","NEWT:1589","NEWT:135622","NCBITaxon:4896","NEWT:6915","NEWT:3649","NEWT:101510","NEWT:28903","NEWT:3880","NEWT:272559","NEWT:28909","NEWT:515849","NEWT:3641","NEWT:383379","NEWT:466585","NEWT:10029","NEWT:913645","NEWT:1000589","NEWT:85963","NEWT:85962","NEWT:143361","NEWT:317447","NEWT:7955","NEWT:7959","NEWT:2261","NEWT:31156","NEWT:398580","NEWT:4565","NEWT:1264690","NEWT:515619","NEWT:192875","NEWT:34305","NEWT:59729","NCBITaxon:183674","NEWT:224308","NEWT:84645","NEWT:626528","NEWT:3347","NEWT:139927","NEWT:4558","NEWT:209285","NEWT:5888","NEWT:211586","NEWT:747078","NEWT:1283","NEWT:931281","NEWT:4550","NEWT:1000561","NEWT:294381","NEWT:197","NEWT:1390363","NEWT:77133","NEWT:288705","NCBITaxon:79824","NEWT:4787","NCBITaxon:4563","NEWT:5755","NEWT:44689","NEWT:3218","NEWT:5759","NEWT:1736231","NEWT:556182","NEWT:1270","NEWT:374990","NEWT:498217","NEWT:156471","NEWT:2242","NEWT:4784","NEWT:11320","NEWT:360106","NEWT:156476","NEWT:286","NEWT:391619","NEWT:360104","NEWT:287","NEWT:246197","NEWT:10117","NEWT:10239","NEWT:10116","NEWT:1280","NEWT:1735272","NEWT:83334","NEWT:83332","NEWT:44685","NEWT:317513","NEWT:1148","NEWT:580240","NEWT:5508","NEWT:294128","NEWT:11676","NEWT:55571","NEWT:35500","NEWT:1140","NEWT:100226","NEWT:4530","NEWT:1143","NEWT:4896","NEWT:75058","NEWT:13616","NEWT:1390","NEWT:1094343","NEWT:1336795","NEWT:296543","NEWT:316435","NEWT:1773","NEWT:1895","NEWT:1182590","NEWT:3712","NEWT:82380","NEWT:105023","NEWT:866628","NEWT:935293","NEWT:64152","NEWT:4924","NEWT:749200","NEWT:375146","NEWT:990346","NEWT:145953","NEWT:257309","NEWT:100816","NEWT:263","NEWT:230741","NEWT:52283","NEWT:284812","NEWT:8175","NCBITaxon:1313","NEWT:43330","NEWT:1603293","NEWT:408169","NEWT:44544","NEWT:47946","NEWT:4911","NEWT:645463","NEWT:3702","NEWT:129249","NEWT:243277","NEWT:990119","NEWT:2850","NEWT:408172","NEWT:408170","NEWT:493760","NEWT:106590","NEWT:260710","NEWT:257313","NEWT:400772","NEWT:3708","NEWT:128161","NEWT:332648","NEWT:106592","NEWT:536231","NEWT:46704","NEWT:1436733","NEWT:460519","NEWT:1187947","NEWT:1432138","NEWT:269796","NEWT:10312","NEWT:1424507","NCBITaxon:1773","NEWT:9598","NEWT:8030","NEWT:9483","NEWT:1639","NEWT:188229","NEWT:3818","NEWT:480","NEWT:4909","NEWT:67767","NEWT:759272","NEWT:432359","NEWT:46835","NEWT:1182263","NEWT:109757","NEWT:943146","NEWT:2711","NEWT:300852","NEWT:1502","NEWT:376686","NEWT:95486","NEWT:9103","NEWT:1883446","NEWT:29159","NEWT:253","NEWT:10306","NCBITaxon:2759","NEWT:1233435","NEWT:93061","NEWT:8022","NEWT:145943","NCBITaxon:4932","NEWT:595536","NEWT:240906","NEWT:593117","NEWT:89920","NEWT:3635","NEWT:5811","NEWT:235443","NEWT:180923","NEWT:108458","NEWT:272623","NEWT:272624","NEWT:411483","NEWT:884019","NEWT:198215","NEWT:411490","NEWT:983964","NEWT:118499","NEWT:169963","NEWT:32644","NEWT:527796","NEWT:225117","NEWT:499175","NEWT:109779","NEWT:43665","NEWT:1266464","NEWT:476272","NEWT:3747","NEWT:195051","NEWT:367830","NEWT:1255228","NEWT:178616","NEWT:649908","NEWT:410289","NEWT:373153","NEWT:375451","NEWT:352472","NEWT:357","NEWT:1071661","NEWT:360094","NEWT:470","NEWT:41364","NEWT:1313","NEWT:411469","NEWT:84023","NEWT:559292","NEWT:39491","NCBITaxon:5811","NEWT:411464","NEWT:411460","NEWT:2014887","NEWT:2762","NEWT:1174673","NEWT:1328426","NEWT:562","NEWT:411470","NEWT:33952","NEWT:2094720","NCBITaxon:2697049","NEWT:571256","NEWT:28038","NEWT:1663","NEWT:1423","NEWT:4932","NEWT:3603","NEWT:2759","NEWT:3847","NEWT:38293","NEWT:327159","NEWT:178876","NEWT:1660","NEWT:327160","NEWT:573","NEWT:9031","NEWT:1872122","NEWT:7091","NEWT:108931","NEWT:241368","NEWT:42528","NEWT:190802","NEWT:9778","NEWT:150475","NEWT:303","NEWT:9417","NEWT:7111","NEWT:347515","NEWT:1216979","NEWT:7237","NEWT:5180","NEWT:256737","NEWT:9541","NEWT:115104","NEWT:1121114","NEWT:663","NEWT:1081927","NEWT:1238993","NEWT:67825","NEWT:185579","NEWT:941442","NEWT:220668","NEWT:13076","NEWT:1821314","NEWT:1249668","NEWT:7108","NEWT:317","NEWT:5286","NEWT:7227","NEWT:7469","NEWT:885318","NEWT:9402","NEWT:9644","NEWT:415540","NEWT:550","NEWT:675060","NEWT:4081","NEWT:334542","NEWT:554","NEWT:27592","NEWT:98334","NEWT:426428","NEWT:63459","NEWT:1276815","NEWT:36185","NEWT:588858","NEWT:9639","NCBITaxon:11071","NEWT:242231",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